LOCUS       NZ_QOIM01000060         9736 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Streptomyces reniochalinae strain LHW50302
            NODE_35_length_9736_cov_16.3354_ID_69, whole genome shotgun
            sequence.
ACCESSION   NZ_QOIM01000060
VERSION     NZ_QOIM01000060.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces reniochalinae
  ORGANISM  Streptomyces reniochalinae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Li,L.
  TITLE     Streptomyces reniochalinae sp. nov. and Streptomyces diacarnus sp.
            nov. from marine sponges
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   Li,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2018) Department of Pharmacy, Ren Ji Hospital
            School of Medicine, Shanghai Jiao Tong University, Pujian, Pudong,
            Shanghai 200127, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-FEB-2016
            Assembly Method                   :: A5-misseq v. MARCH-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003323735.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 01:54:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,751
            CDSs (total)                      :: 6,669
            Genes (coding)                    :: 6,475
            CDSs (with protein)               :: 6,475
            Genes (RNA)                       :: 82
            rRNAs                             :: 6, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 4, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 66 of 194
            Pseudo Genes (incomplete)         :: 154 of 194
            Pseudo Genes (internal stop)      :: 16 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:03:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9736
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QOIM01000060.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..9736
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NI-siderophore"
                     /region_number="1"
                     /rules="IucA_IucC"
                     /tool="antismash"
     rRNA            <1..81
                     /db_xref="RFAM:RF02541"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /locus_tag="DQ392_RS33820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /old_locus_tag="DQ392_33800"
                     /product="23S ribosomal RNA"
     gene            <1..81
                     /locus_tag="DQ392_RS33820"
                     /old_locus_tag="DQ392_33800"
     source          1..9736
                     /collected_by="Lei Li"
                     /collection_date="2015-04-30"
                     /country="China: Xisha"
                     /culture_collection="CCTCC:AA 2018013"
                     /culture_collection="DSM:106194"
                     /db_xref="taxon:2250578"
                     /identified_by="Lei Li"
                     /isolation_source="marine sponge"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces reniochalinae"
                     /strain="LHW50302"
                     /submitter_seqid="NODE_35_length_9736_cov_16.3354_ID_69"
                     /type_material="type strain of Streptomyces reniochalinae"
     cand_cluster    1..9736
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="IucA_IucC"
                     /kind="single"
                     /product="NI-siderophore"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9736
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2242:4120](-)"
                     /cutoff="20000"
                     /detection_rule="IucA_IucC"
                     /neighbourhood="14000"
                     /product="NI-siderophore"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(2243..4120)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="IucA_IucC"
                     /neighbourhood="14000"
                     /product="NI-siderophore"
                     /protocluster_number="1"
     rRNA            202..318
                     /db_xref="RFAM:RF00001"
                     /gene="rrf"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /locus_tag="DQ392_RS33825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /old_locus_tag="DQ392_33805"
                     /product="5S ribosomal RNA"
     gene            202..318
                     /gene="rrf"
                     /locus_tag="DQ392_RS33825"
                     /old_locus_tag="DQ392_33805"
     misc_feature    451..466
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     gene            1336..2109
                     /locus_tag="DQ392_RS34915"
     CDS             1336..2109
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123538.1"
                     /locus_tag="DQ392_RS34915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_220182693.1"
                     /transl_table=11
                     /translation="MPIDEDVTGEELDKDVKQELKSLPKTLAEDVARNLVMVARLLDED
                     PERAYAYAKVALRLASRVPSVREAAGFAAYAVEKYAEALAEFRAARRMTGLQHLWPVMA
                     DCERGLGRPEKALAMAGEPEVRQLDKAGQVEMRLVAAGARRDMGQADAAVVTLQSPELA
                     STSVQPWTARLRYAYADALLEVGREDEAREWFAKALEADQSGVTDASDRLAELDGVEFI
                     DALDGDEDDQDDVDEPAGGAAGGTGRSDEGQVCEG"
     gene            complement(2243..4120)
                     /locus_tag="DQ392_RS33835"
                     /old_locus_tag="DQ392_33815"
     CDS             complement(2243..4120)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NI-siderophore: IucA_IucC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468084.1"
                     /locus_tag="DQ392_RS33835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_33815"
                     /product="IucA/IucC family siderophore biosynthesis
                     protein"
                     /protein_id="WP_114019530.1"
                     /sec_met_domain="IucA_IucC (E-value: 3.9e-76, bitscore:
                     244.7, seeds: 168, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLPTTPQPTPPHAAISHLTPERWARATRLLVRKALAEFTHERLL
                     TPTPLDEAEQEAAQEPAHGTAPVDTLRGDGARRYAVRSDDGATEYRFTARRFALDHWQV
                     EADSISRHHDGTELPLDALAFITELRTSLGLGDDILPVYLEEISSTLASTAYKLAAPPT
                     TAAELAAAGFQDIESGMTEGHPCFIANNGRLGFDIAEYRAYAPETGSPVRLIWLAARRD
                     RTTFTACADLTYDKLVLSELSGATLRRFSAALTEQGLDMDDYHLVPVHPWQWWNKLSVT
                     FAAEVAQRHLVPLGHGDDEHLAQQSIRTFFNATQPNKHYVKTALSVLNMGFLRGLSASY
                     MEATPAINDWLADLVAGDETLRATGLGVLRERAAVGYHPAPYEAAAPKGSPYLKMLAAL
                     WRESPMPLLRDGEKPATMASLLHTDRDGNSLAAALIHGSGLEPTEWLRSYLTAYLTPLL
                     HCFYAYDLVFMPHGENVILVLDERGVPRRALFKDLAEEIAVMSEDAELPPEVERVRADV
                     PEDMKILSLFTDVFDCFFRFLAPVLDTAGVIDEDTFWATVADVAKEYQKSTPHLAERHA
                     RYDLFADEFALSCLNRLQLRNNQQMVDLQDPAGALQLTGTLKNPLAAHR"
     gene            complement(4117..4698)
                     /locus_tag="DQ392_RS33840"
                     /old_locus_tag="DQ392_33820"
     CDS             complement(4117..4698)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1203: putative siderophore biosynthesis protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019354086.1"
                     /locus_tag="DQ392_RS33840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_33820"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_114019531.1"
                     /transl_table=11
                     /translation="MTTADATVFTRTDAELGVFALRPVDPLTDAELLHTWVTHPKAAFY
                     LLQDATLPDIEREFMGIAASPHHDAFLGHHNGEPAFLMERYDPRHIELAGLYPHADGDV
                     GMHFLCAPTEEPVHGFTRAVITTVMAMLFSDPGTHRVVVEPDVRNTAVHALNEAVGFRV
                     ETTLTKPEKEALLSFCTREQFQAATGVTPA"
     gene            complement(4695..6038)
                     /locus_tag="DQ392_RS33845"
                     /old_locus_tag="DQ392_33825"
     CDS             complement(4695..6038)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1080: lysine/ornithine N-monooxygenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537037.1"
                     /locus_tag="DQ392_RS33845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_33825"
                     /product="lysine N(6)-hydroxylase/L-ornithine
                     N(5)-oxygenase family protein"
                     /protein_id="WP_114019532.1"
                     /transl_table=11
                     /translation="MTHDAPVHDFVAIGLGPFNLGLACLTEPIPDLNGVFLDDKPEFTW
                     HPGMMLEDSTLQTPFMSDLVTLADPTSPFSFLNYLKESGRLYSFYIRESFYPLRAEFSD
                     YCHWAADRLADGTVRFGHRVVHIAHDESDGLYTVHAEHLPTGERRTFRAPKLILGTGTP
                     PHLPEPCQDLGGDLIHNSRYLEAKEALQKKRSITLVGSGQSAAEIYYDLLQSIDDHGYS
                     LNWLTRSPRFFPLEYTKLTLEMTSPEYVDYFHSLPPDTRDSLVASQKNLYKGINSELID
                     AIFDLLYQKNRVRSCPTRLMTNTALTDASWDADAGAYTLGLRQEEQKRDFSLTTEGLIL
                     ATGYRYRRPAFLDAIADRIEWDPRGRFAVRRDYSVDTAGQEIYVQNAELHTHGFVAPDL
                     GMAAYRNSSIIRALLGREVYPIERSIAFQEFAAPLADGTPATDAEAAS"
     gene            complement(6073..7587)
                     /locus_tag="DQ392_RS33850"
                     /old_locus_tag="DQ392_33830"
     CDS             complement(6073..7587)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1180: Decarboxylase, pyridoxal-dependent"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468081.1"
                     /locus_tag="DQ392_RS33850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_33830"
                     /product="aspartate aminotransferase family protein"
                     /protein_id="WP_114019533.1"
                     /transl_table=11
                     /translation="MSSRSHLLNDRTAEHYRRSLHDGVERVAEKLAGAGQPFTGVTPAE
                     LAPTVSAVDLEQPLGTAREALDELERVYLRDAVYFHHPRYLAHLNCPVVIPAVLGEAVL
                     TAINSSLDTWDQSAGGTLIERRLVEWTAHRIGFGRAADGIFTSGGSQSNLQALLLAREE
                     TCRLVRKNSETSLSTPQILPKLRILTSECSHFSIEKSAVLLGLGPEAVIPVACDERRRM
                     RADALAEQLARCEREGHTVMAVVATAGTTDFGSIDPLPDIADLCAATGVWLHVDAAYGC
                     GLLTSRRRRALLTGIERADSVTVDYHKSFFQPVSSSAVLVRDKDTLRHVTYHADYLNPT
                     HSVEKGLPNQVDKSIQTTRRFDALKLWMTLRVMGADAVGELFDEVVDRAADAWELLDAD
                     DRFEVVVTPELSTLVFRYVPAGGPDVSGTGQALTDQANLHARDALFASGEAIVAGTVVD
                     GRHHLKFTLLNPETSLADIATVLDLIAGHAARYLDAHTPALSAAQA"
     misc_feature    7635..7653
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: medium, score: 29.62"
                     /tool="antismash"
     misc_feature    complement(7640..7658)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: medium, score: 27.6"
                     /tool="antismash"
     gene            complement(7796..9100)
                     /locus_tag="DQ392_RS33855"
                     /old_locus_tag="DQ392_33835"
     CDS             complement(7796..9100)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607799.1"
                     /locus_tag="DQ392_RS33855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_33835"
                     /product="DUF1015 domain-containing protein"
                     /protein_id="WP_114019534.1"
                     /transl_table=11
                     /translation="MTARGLRLAPFRGLRYAPEKVGSLAAVTSPPYDVVVRPDGLRQLE
                     TADPYNIVRLILPQAADPEVRHRQAAETLRRWQADGVLVRDPSPALYVYEQREGELLQR
                     GLIGALRLTPPEDGIVLPHEDVIDEVVADRAALMRQMAANPEPLLLSYRGSGSRTGATA
                     VIERATRRPPLVATTTEDGFSHRLWAVTDPAELAAVDSDLSVRRALIADGHHRWATYLR
                     LREERGRRDGSSPWDHGLVLLVDTARYPLQVRAIHRVLPRLPLEKALDLLSRAGEGFGE
                     RGAFRVRRLRVPTPAKALGALDEAAGNAFLLSAGDGVFHLLEGPAPRLLERTVPAGRPA
                     AWRELDATVLHATLLDALWQVPDSPADIGYLHHTEAALSQAAACGGTAVLMRPVAEERV
                     RALAEQGVTMPRKSTSFGPKPATGLVLRTLADAPH"
     gene            9297..>9736
                     /locus_tag="DQ392_RS33860"
                     /old_locus_tag="DQ392_33840"
                     /pseudo=""
     CDS             9297..>9736
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002341.1"
                     /locus_tag="DQ392_RS33860"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_33840"
                     /product="HAD family hydrolase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MHASPYGRAAATRPQPSERPLHECYDTALLDLDGVVYAGGEALPH
                     AVGSLAAARKSGVRLAYVTNNAARTPQAVAAHLTQLGVAAGPDEIVTSAQAVARLVAEQ
                     LPEGARVLAVGAEGLRVALRERGLTVVESADDEPAAVAQGYG"
ORIGIN
        1 atgacggggt tgataggccg gatctggaag cccagtaatg ggtggaggtg accggtacta
       61 ataggccgag ggcttgtcca tagaggctcg cgtccactgt gtgggttctg agacaacaac
      121 cgcacttcta agtgaagagt gtgcttgttc gctttgagac tccgggacct ggatcgaaga
      181 tccgggcgtc ccgatcagag tttcggtggt catagcgtga gggaaacgcc cggttacatt
      241 ccgaacccgg aagctaagcc tttcagcgcc gatggtactg catgcgggag cgtgtgggag
      301 agtaggacgc cgccgaacaa tctttatggc ccttggcctc cagcgtgcat gcgctggagg
      361 tcaagggcct tttttgtttc ctctccgcat tcccggagag cgttgttcca ctgcctgcgc
      421 gaggatcgtg tccaggcacc gcagacagga gtcacgtcga tgtcccccag ctcctccgac
      481 gaccgcgacc gtccagaacg ccggccccgg cagtccggtt cagggccgcg ccgcgatgac
      541 gcctcccgct cgggtggtcc gcgcggtccg cggcgtgagg acggcggccg cggcggccgc
      601 agcggtggtt tccgccccgg cggtggcttc cgctcgggcg accgggacgg gcgcgatacg
      661 cgcggcggac ggggcgaccg cgatcggcct cagcgctccg gcggtggctt ctcccgtgat
      721 gaccgtcctc gtgacgaccg tcgccgtgat gaccgtccgc gcggcggacg ggacgagcgg
      781 ccgggccagg ggccccggcg cgacggtgac cgcgaccgcg gcttctcccg tcgcgacgac
      841 cgcgatggcg gttcgttccg tcgcgatgac cgtgaccgtg gctcgtatcg ccgtgacgac
      901 cgcgaccgcg atcagagccg tgaccgtggc tcgtaccgtc gcgacgaccg cgaccgtgac
      961 cgtggctcgt accgccgtga cgaccgcgac cgtggctcgt tccgtcgcga cgaccgcgac
     1021 cgtggttcgt tccgtcggga cgaccgcgac agggggcgta gccgtgacgg cgaccgtgat
     1081 cgcgacaggg gtcggccgca gagtgggccg ggggctcggc gggacgaggg gcgggaccgt
     1141 gggcgtcggc cgtacggggg ccagggcggc ggccgtgacg gccgggacga gcgtcggggt
     1201 ggtttccggc gggacgacga ccggtcgggc ggattccggc gcggtggcgg cgatcgtggg
     1261 cggccgtacg ggcgggacgg gcgggacgac cgggagcgtg gtcgtgaccg gcaggaccgt
     1321 gagccgctgc ggcggctgcc gatcgacgag gacgtcaccg gcgaggagct ggacaaggac
     1381 gtcaagcagg agttgaagag cctgccgaag acgctggcgg aggacgtcgc gcggaacctg
     1441 gtgatggtgg cgcggctgct ggacgaggac cccgagcggg cgtacgcgta cgcgaaggtg
     1501 gcgctgcggc tggcgtcgag ggtgccgtcg gtgcgtgagg ctgcgggctt cgccgcgtac
     1561 gcggtggaga agtacgcgga ggcgttggcg gagttccggg ccgcgcgccg gatgaccggg
     1621 ttgcagcatc tgtggccggt gatggcggac tgcgagcgcg ggctgggccg tcctgagaag
     1681 gcgttggcca tggcgggtga gcccgaggtg cggcagctcg acaaggcggg gcaggtcgag
     1741 atgcggctcg tcgcggcggg ggcccggcgt gacatgggcc aggcggacgc cgctgtggtg
     1801 acgttgcaga gcccggagct ggcgtccacg tccgtgcagc cgtggacggc gcggctgcgg
     1861 tacgcgtacg cggacgcgtt gctcgaggtc ggtcgtgagg acgaggcgcg cgagtggttc
     1921 gccaaggcat tggaggccga tcagagcggg gtgacggacg cctcggaccg gctcgcggag
     1981 ctggacggtg tggagttcat cgacgctctg gacggggacg aggacgacca ggacgacgtg
     2041 gacgagcccg ccggaggcgc cgcaggcggg accgggcgga gcgacgaggg gcaggtctgc
     2101 gagggctgac cccgggccac tcggctcccg gagaccctgg cgcggtgtgc tgccgcgcga
     2161 cgagagcgag tacggagggc gggccccgtt cggggcccgc cctccgtact cgtgcgcgct
     2221 cgtactcgtt acgtgctcgt cctcacctgt gcgcggccag cgggttcttc agggtgccgg
     2281 tgagctggag cgcgccggca gggtcctgga ggtcgaccat ctgctggttg ttgcgcagct
     2341 ggaggcggtt gaggcaggag agggcgaact cgtccgcgaa gaggtcgtat cgcgcgtggc
     2401 gctcggccag gtgcggggtg gacttctggt actccttggc gacgtcggcg acggtggccc
     2461 agaaggtgtc ctcgtcgatg accccggccg tgtccaggac gggtgcgagg aagcggaaga
     2521 agcagtcgaa gacgtccgtg aagagggaga ggatcttcat gtcctcgggg acgtccgcgc
     2581 ggacgcgctc gacctcgggg gggagttcgg cgtcctcgct catgacggcg atctcctcgg
     2641 ccaggtcctt gaagagggcc cggcggggga cgccccgctc gtccaggacg aggatgacgt
     2701 tctcgccgtg cggcatgaac accaggtcgt aggcgtagaa gcagtgcagc agcggggtga
     2761 ggtaggcggt caggtaggag cgcagccact cggtgggttc gagccccgag ccgtggatga
     2821 gggcggcggc cagggagttc ccgtcgcggt cggtgtgcag gagcgaggcc atggtggcgg
     2881 gcttctcgcc gtcacgcagg aggggcatgg ggctctcgcg ccacagcgcg gcgagcatct
     2941 tcaggtaggg ggagcccttg ggggcggctg cctcgtaggg ggcggggtgg tagccgacgg
     3001 cggcgcgttc gcgcaggacg ccgagaccgg tggcgcggag cgtctcgtcg ccggccacga
     3061 ggtcggccag ccagtcgttg atggccgggg tcgcttccat gtaggaggcg gacaggccgc
     3121 gcaggaagcc catgttgagg acggagagcg cggtcttcac gtagtgcttg ttgggctggg
     3181 tggcgttgaa gaaggtgcgg atggactgct gggcgaggtg ctcgtcgtcg ccgtggccga
     3241 gcggtaccag gtggcgctgg gcgacctcgg cggcgaaggt gaccgacagt ttgttccacc
     3301 actgccaggg gtggacgggc acgaggtggt agtcgtccat gtccaggccc tgttcggtca
     3361 gggcggcgga gaagcggcgg agggtggcgc cggagagctc ggagaggacg agtttgtcgt
     3421 aggtgaggtc ggcgcaggcg gtgaaggtgg tgcggtcgcg gcgtgcggcg agccagatga
     3481 gccggacggg ggagccggtc tcgggggcgt aggcgcggta ttcggcgatg tcgaaaccga
     3541 ggcggccgtt gttggcgatg aagcaggggt ggccctcggt catgccggac tcgatgtcct
     3601 ggaagccggc ggcggcgagc tcggccgcgg tggtgggggg cgccgcgagt ttgtaggcgg
     3661 tgctggccag ggtcgagctg atctcctcca ggtagacggg gaggatgtcg tcgccgaggc
     3721 cgagtgaggt ccgcagttcg gtgatgaagg cgagcgcgtc gagggggagt tccgtgccgt
     3781 cgtggtggcg gctgatgctg tcggcttcga cctgccagtg gtcgagggcg aagcggcggg
     3841 cggtgaagcg gtactcggtg gcgccgtcgt cggagcggac ggcgtagcgg cgtgcgccgt
     3901 ccccccggag cgtgtcgaca ggcgcggtcc cgtgtgcggg ttcctgtgca gcttcctgtt
     3961 ccgcctcgtc cagcggggtg ggggtcagca gccgctcgtg ggtgaactcg gcgagggcct
     4021 tgcgcaccag gagtcgggtg gcgcgggccc agcgttcggg tgtgaggtgg cttatggccg
     4081 cgtgcggtgg ggtgggctgc ggggtggtgg ggaggttcat gcgggggtca ctcctgtggc
     4141 ggcctggaac tgctcgcggg tgcagaagct gaggagggct tccttctccg gcttggtgag
     4201 ggtggtctcg acgcggaagc cgaccgcctc gttgagggcg tgcacggcgg tgttgcgcac
     4261 gtcgggttcg acgacgacgc ggtgggtgcc ggggtcggag aagagcatcg ccatgacggt
     4321 ggtgatgacg gcacgggtga agccgtgcac gggctcttcg gtgggggcgc acaggaagtg
     4381 catgccgacg tcgccgtcgg cgtgcgggta gaggccggcg agttcgatgt ggcgcgggtc
     4441 gtagcgttcc atcaggaagg cgggttcgcc gttgtggtgg ccgaggaagg cgtcgtggtg
     4501 gggcgaggcc gcgatgccca tgaactcgcg ttcgatgtcc gggagtgtcg cgtcctggag
     4561 caggtagaac gccgccttgg ggtgggtgac ccaggtgtgc agcagctccg cgtcggtgag
     4621 cgggtcgacg gggcggaggg cgaagacgcc cagttccgcg tcggtgcggg tgaagacggt
     4681 ggcgtcggcg gtggtcacga ggcggcctcc gcgtcggtgg cgggtgtgcc gtcggccagg
     4741 ggggcggcga actcctggaa ggcgatggac cgctcgatcg ggtacacctc gcggccgagc
     4801 agcgcgcgga tgatgctgga gttgcggtag gcggccatgc ccaggtcggg ggcgacgaag
     4861 ccgtgggtgt gcagttccgc gttctgtacg tagatctcct gcccggcggt gtcgacgctg
     4921 tagtcgcggc gtacggcgaa gcgtccgcgc gggtcccact cgatgcggtc ggcgatcgcg
     4981 tcgaggaagg cggggcgccg gtagcggtag ccggtggcca ggatcaggcc ctcggtggtg
     5041 agggagaagt cgcgcttctg ctcctcctgg cgcagtccga gggtgtaggc gccggcgtcc
     5101 gcatcccagg aggcgtcggt cagcgcggtg ttggtcatca gccgggtcgg gcaggagcgg
     5161 acgcggttct tctggtagag caggtcgaag atggcgtcga tcagctcgga gttgatgccc
     5221 ttgtagaggt tcttctgcga ggcgacgaga ctgtcgcggg tgtcgggcgg gagggagtgg
     5281 aagtagtcca cgtactccgg cgaggtcatc tccagtgtca gtttggtgta ttcgaggggg
     5341 aagaagcggg gggagcgggt cagccagttg agcgagtagc cgtggtcgtc gatgctttgg
     5401 aggaggtcgt agtagatctc ggcggcgctc tggccgctgc ccaccagggt gatgctccgc
     5461 ttcttctgga gggcttcctt ggcttccagg tagcgggagt tgtggatcag gtcgccgccg
     5521 aggtcctggc agggttcggg caggtggggc ggtgtgccgg tgcccaggat gagcttcggt
     5581 gcgcggaagg tgcggcgctc gcccgtgggc aggtgctcgg cgtggacggt gtagagcccg
     5641 tcgctctcgt cgtgtgcgat gtggacgacg cggtggccga agcgcacggt gccgtccgcg
     5701 agccggtcgg cggcccagtg gcagtagtcg ctgaactcgg cgcggagcgg gtagaagctc
     5761 tcgcgtatgt agaaggagta gagccgtccc gattccttga ggtagttgag gaaggagaac
     5821 ggtgaggtcg ggtccgcgag agtgaccagg tcggacatga agggggtctg gagggtgctg
     5881 tcctccagca tcatgccggg gtgccaggtg aattccggct tgtcgtcgag gaagacgccg
     5941 ttcaggtccg gtatcggctc ggtcaggcag gcgaggccca ggttgaaggg gcccagtccg
     6001 atggcgacga agtcgtgtac gggggcgtcg tgggtcacgt cggggctccg gggtgtgggg
     6061 aggttgcggc ggtcaggcct gggcggcgct gagggcgggg gtgtgggcgt cgaggtaccg
     6121 ggcggcgtgg ccggcgatca ggtcgaggac ggtggcgatg tcggccagtg aggtctcggg
     6181 gttgagcagg gtgaacttga ggtggtggcg gccgtcgacc acggtgccgg cgacgatggc
     6241 ctcgcccgag gcgaacaggg cgtcgcgggc gtgcaggttg gcctggtcgg tgagggcctg
     6301 gccggtgccg gagacgtccg ggccgcccgc ggggacgtag cggaagacga gggtggacag
     6361 ctcgggggtg acgacgacct cgaacctgtc gtcggcgtcg agcagttccc aggcgtcggc
     6421 ggcgcggtcg accacctcgt cgaagagttc gcccacggcg tcggcgccca tgacgcgcag
     6481 ggtcatccac agtttgagcg cgtcgaagcg gcgcgtggtc tgtatcgatt tgtcgacctg
     6541 gttgggcagg cccttctcga cgctgtgggt ggggttgagg tagtcggcgt ggtaggtgac
     6601 gtggcgcagg gtgtccttgt ccctgaccag gacggcgctg gaggagaccg gctggaagaa
     6661 cgacttgtgg tagtcgaccg tcaccgagtc ggcgcgctcg atgcccgtca gcagcgcgcg
     6721 gcggcgccgc gaggtgagca gcccgcagcc gtaggcggcg tccacgtgca gccagacgcc
     6781 cgtcgccgcg cacaggtcag cgatgtccgg cagcgggtcg atgctgccga agtcggtggt
     6841 gccggccgtg gcgacgacgg ccatcaccgt gtgtccctcg cgctcgcagc gcgccagttg
     6901 ctccgcgagc gcgtcggcgc gcatccggcg ccgctcgtcg caggccaccg ggatgacggc
     6961 ttcggggccc aggccgagca ggaccgccga cttctcgatg ctgaagtggc tgcactcgga
     7021 ggtgaggatg cgcagcttcg gcaggatctg cggggtgctc agcgacgtct cggagttctt
     7081 gcgcaccagc cggcaggtct cctcgcgggc cagcagcagc gcctggaggt tggactggga
     7141 gccgccgctg gtgaagatgc cgtccgccgc ccgcccgaag ccgatccggt gcgccgtcca
     7201 ctcgacgagg cggcgctcga tcagggtgcc gcccgcgctc tggtcccagg tgtccagcga
     7261 ggagttgatc gcggtgagga ccgcttcgcc gaggacggcc ggtatcacca cggggcagtt
     7321 gaggtgcgcg aggtagcggg ggtggtggaa gtagacggcg tcgcgcaggt acacccgctc
     7381 cagctcgtcc agcgcctcgc gggccgtacc caggggttgc tccagatcga cggcggagac
     7441 ggtgggggcg agctccgccg gggtgacgcc ggtgaagggt tgtccggcgc cggcgagttt
     7501 ctcggcgacg cgctcgacac cgtcgtgcag ggagcgcctg tagtgctccg ctgtcctgtc
     7561 gttgagcagg tgggacctgc tgctcatgaa gtgtcctcct gacacggacg tggcgcaccg
     7621 cgcggctcgc gcggtcaggt aaggctagcc taaccgggcc ggtcagccgg tgtgcaaggg
     7681 ggactcctcg gtcagccgtg cgccgatcgg gcagggcgga attgcgcggc aatgcccgca
     7741 tgggggaggg gagttggacg cctcggcaga cgggtggccg aggcggcgcc gctggtcagt
     7801 gcggtgcgtc ggccagagtg cgcagcacga gtccggtggc cggtttcggg ccgaacgaag
     7861 tggacttacg cggcatcgtg acgccctgct ccgccagtgc ccgcaccctc tcctcggcaa
     7921 ccggccgcat cagcacggcc gtgccgccgc acgccgccgc ctggctgagc gcggcctcgg
     7981 tgtggtgcag ataaccgatg tccgccgggg agtccgggac ctgccagagg gcgtccagca
     8041 gcgtcgcgtg caggaccgtc gcgtccagct cccgccacgc cgcgggccgc cccgccggaa
     8101 ccgtccgctc cagcagccgg ggggccggcc cctcgagcag atggaagacg ccgtcgcccg
     8161 cgctcagcaa gaaggcgttg cccgcggcct cgtccagcgc gcccagcgcc tttgcgggcg
     8221 tcgggacccg gagccggcgc acccggaagg cgccccgctc cccgaatccc tcccccgcac
     8281 gggagagcag gtcgagcgcc ttttccagcg gcagcctggg cagcacccgg tgaatggccc
     8341 gcacctggag cggatagcgc gccgtgtcga ccagcagcac caggccgtgg tcccaggggg
     8401 aggacccgtc ccgccgcccc cgttcctcgc gcagccgcag atacgtcgcc cagcggtgat
     8461 ggccgtccgc gatcagtgct cgccgcaccg acaggtcgga gtcgaccgcc gcgagctcgg
     8521 ccgggtccgt gaccgcccac agccggtgcg agaaaccgtc ctcggtcgtc gtcgccacca
     8581 gcggcgggcg ccgcgtcgcc cgctcgatga cggccgtggc accggtgcgg gagccgctgc
     8641 cccggtagga gagcagcagc ggctcggggt tggcggccat ctggcgcatc agcgcggcac
     8701 ggtcggcgac gacctcgtct atcacgtcct cgtgcggcag cacgatgccg tcctcgggcg
     8761 gggtgagccg cagcgcgccg atgagtccgc gctgcaacag ctcgccctcg cgctgctcgt
     8821 acacgtacag cgcgggagac ggatcgcgga ccagcacgcc gtcggcctgc cagcggcgca
     8881 gcgtctcggc cgcctgacgg tgccgcacct cggggtccgc ggcctgggga aggatgagcc
     8941 gcacgatgtt gtacgggtcg gccgtctcca gctgccgcag cccgtcgggc ctgaccacca
     9001 cgtcgtacgg cggggaggtc acggcagcga ggctgccgac cttctccggc gcgtagcgca
     9061 gtccccggaa cggagcgagt cgaaggccac gggcggtcat ccgtgcatcg tatgacggtg
     9121 cacggtggcg ccgggagtgg ggcacagggg gcgcgcggtg gcggggtccg gtgggacgtg
     9181 ggagaggatt ccatacggcg tccgagccgt gcgggcccgg cgcctgcgcc tcccggtccc
     9241 gcggcgaagg cgcccggagc accacgagaa agagcaccac gagaaagtga gcacccatgc
     9301 acgccagccc gtacggacga gccgccgcga cccgtccgca gcccagcgag cgccccctgc
     9361 acgagtgcta cgacaccgcg ctgctcgacc tggacggagt ggtctacgcc gggggagagg
     9421 cgctgccgca cgccgtcggc tcactggccg ccgcgcggaa gagcggcgtg cgcctcgcct
     9481 acgtcaccaa caacgcggcc cgcaccccgc aggccgtcgc cgcgcacctg acccagctgg
     9541 gcgtggcggc aggaccggac gagatcgtca cctcggccca ggcggtcgcc cggctcgtcg
     9601 ccgaacagct gcccgagggc gcgcgcgtgc tcgccgtcgg cgccgagggg ctgcgggtcg
     9661 cccttcgaga gcgcggcctg accgtcgtcg agtcggccga cgacgagccc gccgccgtgg
     9721 cgcagggcta cggcgg
//
