LOCUS       NZ_QOIM01000051         4596 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Streptomyces reniochalinae strain LHW50302
            NODE_43_length_4596_cov_16.6179_ID_85, whole genome shotgun
            sequence.
ACCESSION   NZ_QOIM01000051
VERSION     NZ_QOIM01000051.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces reniochalinae
  ORGANISM  Streptomyces reniochalinae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Li,L.
  TITLE     Streptomyces reniochalinae sp. nov. and Streptomyces diacarnus sp.
            nov. from marine sponges
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Li,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2018) Department of Pharmacy, Ren Ji Hospital
            School of Medicine, Shanghai Jiao Tong University, Pujian, Pudong,
            Shanghai 200127, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-FEB-2016
            Assembly Method                   :: A5-misseq v. MARCH-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003323735.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 01:54:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,751
            CDSs (total)                      :: 6,669
            Genes (coding)                    :: 6,475
            CDSs (with protein)               :: 6,475
            Genes (RNA)                       :: 82
            rRNAs                             :: 6, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 4, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 66 of 194
            Pseudo Genes (incomplete)         :: 154 of 194
            Pseudo Genes (internal stop)      :: 16 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:03:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4596
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QOIM01000051.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..4552)
                     /locus_tag="DQ392_RS31845"
                     /old_locus_tag="DQ392_31825"
     CDS             complement(<1..4552)
                     /NRPS_PKS="Domain: PKS_DH (162-319). E-value: 6.7e-53.
                     Score: 171.4. Matches aSDomain:
                     nrpspksdomains_DQ392_RS31845_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (622-801). E-value: 4.1e-52.
                     Score: 168.7. Matches aSDomain:
                     nrpspksdomains_DQ392_RS31845_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (906-979). E-value: 6.9e-32. Score:
                     101.6. Matches aSDomain:
                     nrpspksdomains_DQ392_RS31845_ACP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (999-1414). E-value:
                     4.3e-181. Score: 594.4. Matches aSDomain:
                     nrpspksdomains_DQ392_RS31845_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /locus_tag="DQ392_RS31845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_31825"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_220182682.1"
                     /sec_met_domain="PKS_AT (E-value: 6.3e-27, bitscore: 80.8,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 3e-58, bitscore:
                     182.8, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.2e-16, bitscore:
                     44.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 3.2e-229, bitscore:
                     747.0, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 2.1e-93, bitscore:
                     298.6, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDPMLEEFAAVAATLSYHEPVLPIVSTVTGQLVVEGQLTDPGYWV
                     DQVRRAVRFADGVTAADASVFVEIGPDGVLAGLAQQSLDEAAVVVPAVRKDRGEAQAFI
                     EALGRLHTTGVPVDWTSHFGDVATHIDLPTYAFQRERYWLQSGAARGDVSAVGLTTIDH
                     PLVGAVVSTPDTGGMTITGRISLATHPWLADHEVLGTVLVPGTGLLELALQAGHEAGLP
                     EVRELTLQAPMVIPDDSALQLRIAVGDGTLTIHSRDEHDVAGAWTLHAEGVLAADTSTP
                     DVGLTEWPPVGAVSVDVSGVYEGLLGRGYVYGPVFQGLRAVWERGDEVFAEVELPEDGV
                     VDAGRFGLHPAVFDAAMHALLVVGGGDAETVLPFVWSGVRLHAAGASRLRVRISRVSGA
                     MAFADAAGAPVLTVESLVSRPVSAEQLGARNEALFRVEWARCAVAGGSLDGVVAVGAVP
                     GVEGVAECADLAEVGALEEVPSWVVSECCGEVGSGDVVGAVRRSVGGVLDLVQEFLGGV
                     RFAESRLVVVTRGAVAVGSDDEVVPSVAPVWGLVRAAQAEHPGRVVLVDVDGGVESGAV
                     LAGVLASGEPEVAVRAGEVLVPRLVRAEVPASVDEAVGEGPVVGAEGTVLVTGGTGGLG
                     ALASRSLVVEHGVRRLVLASRRGMEAPGAGELVAELEGLGARVEVVACDVADRDAVERL
                     VGSVPAEFPLRGVVHVAGVGDAGLVESLTREGVEGVLGAKVDGAWFLHEVTCELDLAFF
                     VLFSSAGGLVLPAGQGGYAAANVFLDGLAAYRRGLGLVGTSLAFGLWEADTGLTRLLDA
                     GDMERMRRQGLPALSVEEGLAAFDAGLTSSEALLVPLRVDVPALRGRADVPALLRGLAP
                     PRARRAARAAVGGGEESVFGRQLSGLDAGERARVMLELVRGKVAAVLGHTSAVAVEPER
                     AFQDLGFDSLTAVELRNELNAATGLRLPATLVFDYPNAQAVVEYLDEQLGGASASDSSV
                     VAVRAVDDEPIAIVGMACRYPGGVSSPDDLWRLVEDEVDAVSEFPTDRGWDLDRLYDPE
                     PGVPGKSYTRAGSFLYEAGDFDPGFFGISPREALGMDPQQRLLLETSWEAFERAGVDPR
                     SRRGSRTGVFAGLMYHDYGLGIPGATSTGSLLSGRVSYTFGFEGPAVTVDTACSSSLVA
                     LHLAVQALRSGECDLALAGGATVMSTPGTFVEFSRQRGLSPDGRCKAFASSADGTGWGE
                     GVGVLLVERLSDARRNGHPVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRQALANAAL
                     SPSDIDAVEAHGTGTTLGDPIEAQALIATYGQDRPAGRPLWLGSVKSNFGHTQAAAGVA
                     GVIKMVMAMRRGVLPRSLHVDEPSSHVDWSAGAVELLTEARQWTTSDRPRRAGISSFGI
                     SGTNAHVVIEGAAEAPAETSTADVPDLPFVPWLVSGASAEALSAQAARLREWCAGEPDL
                     DPRAVGSALVTGRASLDHRAVVVGAGRVELLAGLEKLAGGEVWPGVVTGSGSGGMV"
     source          1..4596
                     /collected_by="Lei Li"
                     /collection_date="2015-04-30"
                     /country="China: Xisha"
                     /culture_collection="CCTCC:AA 2018013"
                     /culture_collection="DSM:106194"
                     /db_xref="taxon:2250578"
                     /identified_by="Lei Li"
                     /isolation_source="marine sponge"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces reniochalinae"
                     /strain="LHW50302"
                     /submitter_seqid="NODE_43_length_4596_cov_16.6179_ID_85"
                     /type_material="type strain of Streptomyces reniochalinae"
     region          1..4596
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..4596
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4596
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:4552](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..4552)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     aSDomain        complement(311..1555)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DQ392_RS31845_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="4.30E-181"
                     /label="DQ392_RS31845_PKS_KS.1"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="1414"
                     /protein_start="999"
                     /score="594.4"
                     /tool="antismash"
                     /translation="IAIVGMACRYPGGVSSPDDLWRLVEDEVDAVSEFPTDRGWDLDRL
                     YDPEPGVPGKSYTRAGSFLYEAGDFDPGFFGISPREALGMDPQQRLLLETSWEAFERAG
                     VDPRSRRGSRTGVFAGLMYHDYGLGIPGATSTGSLLSGRVSYTFGFEGPAVTVDTACSS
                     SLVALHLAVQALRSGECDLALAGGATVMSTPGTFVEFSRQRGLSPDGRCKAFASSADGT
                     GWGEGVGVLLVERLSDARRNGHPVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRQALA
                     NAALSPSDIDAVEAHGTGTTLGDPIEAQALIATYGQDRPAGRPLWLGSVKSNFGHTQAA
                     AGVAGVIKMVMAMRRGVLPRSLHVDEPSSHVDWSAGAVELLTEARQWTTSDRPRRAGIS
                     SFGISGTNAHVVIEGA"
     CDS_motif       complement(530..574)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS31845_0010"
                     /evalue="2.00E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="1341"
                     /protein_start="1326"
                     /score="10.8"
                     /tool="antismash"
                     /translation="GSVKSNFGHTQAAAG"
     CDS_motif       complement(635..679)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS31845_0009"
                     /evalue="3.90E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="1306"
                     /protein_start="1291"
                     /score="12.6"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       complement(1055..1102)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS31845_0008"
                     /evalue="7.30E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="1166"
                     /protein_start="1150"
                     /score="21.0"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     aSDomain        complement(1616..1834)
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DQ392_RS31845_ACP.1"
                     /evalue="6.90E-32"
                     /label="DQ392_RS31845_ACP.1"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="979"
                     /protein_start="906"
                     /score="101.6"
                     /tool="antismash"
                     /translation="LELVRGKVAAVLGHTSAVAVEPERAFQDLGFDSLTAVELRNELNA
                     ATGLRLPATLVFDYPNAQAVVEYLDEQL"
     aSModule        complement(1616..4066)
                     /domains="nrpspksdomains_DQ392_RS31845_PKS_DH.1"
                     /domains="nrpspksdomains_DQ392_RS31845_PKS_KR.1"
                     /domains="nrpspksdomains_DQ392_RS31845_ACP.1"
                     /incomplete
                     /locus_tags="DQ392_RS31845"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       complement(1781..1813)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS31845_0007"
                     /evalue="9.90E+01"
                     /label="PKSI-KS_m6"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="924"
                     /protein_start="913"
                     /score="-1.9"
                     /tool="antismash"
                     /translation="VAAVLGHTSAV"
     aSDomain        complement(2150..2686)
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N found: True"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DQ392_RS31845_PKS_KR.1"
                     /evalue="4.10E-52"
                     /label="DQ392_RS31845_PKS_KR.1"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="801"
                     /protein_start="622"
                     /score="168.7"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="GTVLVTGGTGGLGALASRSLVVEHGVRRLVLASRRGMEAPGAGEL
                     VAELEGLGARVEVVACDVADRDAVERLVGSVPAEFPLRGVVHVAGVGDAGLVESLTREG
                     VEGVLGAKVDGAWFLHEVTCELDLAFFVLFSSAGGLVLPAGQGGYAAANVFLDGLAAYR
                     RGLGLVGTSLAFGLWE"
     CDS_motif       complement(2195..2305)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS31845_0006"
                     /evalue="1.30E-15"
                     /label="PKSI-KR_m4"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="786"
                     /protein_start="749"
                     /score="49.9"
                     /tool="antismash"
                     /translation="LAFFVLFSSAGGLVLPAGQGGYAAANVFLDGLAAYRR"
     CDS_motif       complement(2327..2356)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS31845_0005"
                     /evalue="6.40E-02"
                     /label="PKSI-KR_m3"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="742"
                     /protein_start="732"
                     /score="7.1"
                     /tool="antismash"
                     /translation="AKVDGAWFLH"
     CDS_motif       complement(2582..2683)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS31845_0004"
                     /evalue="8.00E-08"
                     /label="PKSI-KR_m1"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="657"
                     /protein_start="623"
                     /score="24.9"
                     /tool="antismash"
                     /translation="TVLVTGGTGGLGALASRSLVVEHGVRRLVLASRR"
     aSDomain        complement(3596..4066)
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DQ392_RS31845_PKS_DH.1"
                     /evalue="6.70E-53"
                     /label="DQ392_RS31845_PKS_DH.1"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="319"
                     /protein_start="162"
                     /score="171.4"
                     /tool="antismash"
                     /translation="HPLVGAVVSTPDTGGMTITGRISLATHPWLADHEVLGTVLVPGTG
                     LLELALQAGHEAGLPEVRELTLQAPMVIPDDSALQLRIAVGDGTLTIHSRDEHDVAGAW
                     TLHAEGVLAADTSTPDVGLTEWPPVGAVSVDVSGVYEGLLGRGYVYGPVFQGL"
     CDS_motif       complement(3746..3769)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS31845_0003"
                     /evalue="2.70E+00"
                     /label="PKSI-KR_m3"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="269"
                     /protein_start="261"
                     /score="2.3"
                     /tool="antismash"
                     /translation="VAGAWTLH"
     CDS_motif       complement(3905..3982)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS31845_0002"
                     /evalue="1.00E-09"
                     /label="PKSI-DH_m2"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="216"
                     /protein_start="190"
                     /score="30.8"
                     /tool="antismash"
                     /translation="WLADHEVLGTVLVPGTGLLELALQAG"
     CDS_motif       complement(4391..4426)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS31845_0001"
                     /evalue="2.20E-03"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="DQ392_RS31845"
                     /protein_end="54"
                     /protein_start="42"
                     /score="11.5"
                     /tool="antismash"
                     /translation="YWVDQVRRAVRF"
ORIGIN
        1 cgaccatccc gccggaaccc gaacccgtga ccacaccagg ccagacctca cccccggcca
       61 gcttctccaa ccccgccaac aactccaccc gacccgcacc caccaccacc gcacgatggt
      121 cgagactcgc ccgcccggtc acgagcgcac tgcccaccgc gcgcggatcc agatcgggct
      181 caccggcgca ccactcgcgc aggcgcgcgg cctgcgcgct cagtgcctcg gcgctcgcgc
      241 cggacaccag ccacggcacg aagggaaggt cgggcacgtc tgcggtggac gtctcggcgg
      301 gcgcttcagc ggccccctcg atgaccacgt gcgcgttggt gccactgatg ccgaacgagg
      361 agatgccggc cctgcgcggc cggtcgctgg tggtccactg gcgtgcttcg gtgagcagct
      421 cgacggcccc ggccgaccag tccacatgcg agctcggctc atcgacatgc aggctccggg
      481 gcagcacacc ccgccgcatc gccatcacca tcttgatcac acccgccaca ccggcggccg
      541 cttgcgtgtg accgaagttc gacttgaccg agcccagcca cagcggccgg ccggcgggcc
      601 ggtcctggcc gtacgtggcg atgagggcct gcgcctcgat ggggtcgccc agcgtcgttc
      661 ccgtgccgtg cgcctccacc gcgtcgatgt ccgagggaga caaggcggcg ttggcgagcg
      721 cctgccggat gacccgctgc tgggaggggc cgttgggtgc ggtcaggccg ttgctggcac
      781 cgtcctggtt gacggcagag ccccgcacca cggcaaggac cgggtgcccg ttccgccgcg
      841 cgtccgaaag ccgctccacc agcagaacac ccacgccctc gccccagccg gtgccgtccg
      901 ccgacgacgc gaaggccttg caccgcccgt cgggtgacag accccgctgg cgggagaact
      961 ccacgaacgt gccgggggtc gacatcacgg tggcgccgcc ggccagcgcg agatcacact
     1021 caccgctacg caatgcctgt accgcgagat gcagggcaac cagcgacgac gagcacgccg
     1081 tgtccaccgt caccgcgggg ccctcgaacc cgaacgtgta ggaaacccgc ccagaaagca
     1141 gacttcccgt actcgtcgcc ccaggaattc ccaggccgta gtcgtggtac atcagcccgg
     1201 cgaacacacc ggtccggctg ccacgacgcg aacgcgggtc gacaccagca cgctcgaacg
     1261 cctcccacga cgtctccaac aacagacgct gctgcgggtc catccccaga gcctcacggg
     1321 gagagatccc gaagaacccg gggtcgaaat cgccggcctc atacaggaaa ctgccggcgc
     1381 gcgtgtagct cttacccggc actcccggct ccgggtcgta cagccggtca agatcccaac
     1441 cccgatcggt cggaaactcg gagaccgcgt ccacctcgtc ctcgaccagg cgccacaaat
     1501 cgtcaggcga ggacactcca ccggggtagc ggcaggccat gcccacgatg gcgatcggct
     1561 cgtcgtccac tgctcggaca gcgaccaccg agctgtccga tgccgacgcg ccaccgagtt
     1621 gctcgtccag gtactcgaca acggcctggg cattcgggta gtcgaacacc agcgtcgcgg
     1681 ggagccggag gccggtcgcc gcgttcagct cgttacgcag ctcgaccgcg gtcagcgagt
     1741 cgaagcccag gtcctggaac gccctctccg gctcgaccgc caccgccgag gtgtgcccaa
     1801 gaacagccgc caccttcccc cgcaccaact ccaacatcac acgggcacgc tcaccggcat
     1861 ccagccccga cagctgacgc ccgaacaccg actcctcacc accaccgaca gcagcacgag
     1921 ccgcccgacg ggcccgcggc ggcgccaggc cccgcagaag cgccggcaca tccgcccggc
     1981 cacgcaacgc cggaacatcc acccgcaacg gcaccaacag ggcctcggac gaagtcagcc
     2041 cggcatcgaa cgccgccaac ccctcctcaa cagacaacgc cggcaacccc tgacgacgca
     2101 tccgctccat atcccccgca tccaacaacc gcgtcaaccc cgtatccgcc tcccacaacc
     2161 cgaacgccaa cgacgtcccc accaaaccga gcccacggcg ataagccgcc aacccgtcca
     2221 agaacacatt cgccgccgcg tacccaccct ggcccgccgg caacaccaac ccacccgccg
     2281 acgagaacaa cacaaagaac gccagatcca actcacacgt cacctcgtgc aagaaccacg
     2341 ccccatccac cttcgccccc aacacacctt ccacaccctc ccgagtcagc gactcgacca
     2401 accccgcatc ccccaccccc gccacatgca ccacgccccg caacggaaac tccgccggca
     2461 ccgaccccac cagccgctcc acagcatccc ggtccgccac atcacacgcc accacctcaa
     2521 cccgagcccc caacccctcc agctcggcaa ccaactcccc cgcccccgga gcctccatcc
     2581 cccgacggct cgccaacacc agacgccgca caccatgctc aaccaccaaa gagcgcgacg
     2641 ccaacgcacc cagaccaccc gtaccacccg tcaccaacac cgttccctcc gcgcccacca
     2701 ccgggccctc acccacagcc tcatccaccg acgccggcac ctcagcccgc accagacgag
     2761 gcaccaacac ctcacccgcc cggaccgcca cctccggctc accggacgcc aacactcccg
     2821 ccaacaccgc acccgactcc acaccaccgt ccacatccac cagcacgacc cgccccggat
     2881 gctccgcctg cgccgcacgc accaagcccc acaccggcgc cacagacggc accacctcat
     2941 cgtccgaccc caccgcgaca gcaccccgcg tcaccaccac cagacgagac tccgcgaacc
     3001 gcacaccacc caaaaactcc tgcaccagat ccaacacgcc ccccaccgac cgccgcaccg
     3061 cacccaccac gtctcccgaa cccacttcac cgcagcactc cgacaccacc cacgaaggaa
     3121 cctcctccaa cgcccccacc tcagcaaggt ccgcacactc cgcgacaccc tccaccccgg
     3181 gcaccgcgcc gaccgccaca accccgtcca acgacccacc agcaaccgca caccgcgccc
     3241 actccacccg gaacaacgcc tcattccggg cacccaactg ctccgccgac accggccgcg
     3301 acaccaacga ctccaccgtc aacaccgggg cacccgcagc atctgcgaac gccatcgcac
     3361 cactcacccg cgaaatccgc acccgcaacc gcgaagcacc cgcggcatgc aaccgcacac
     3421 cggaccacac gaacggcaac accgtctccg cgtccccacc acccacgacc aacaacgcat
     3481 gcatcgccgc gtcgaacaca gcaggatgca aaccaaaccg acccgcatca acaaccccgt
     3541 cctcaggcaa ctccacctca gcaaaaacct catccccccg ctcccacacc gcacgcaacc
     3601 cctgaaacac cgggccatac acatagcccc gacccaacaa accctcatac acacccgaca
     3661 catcaacaga cacagcacca accggcggcc attccgtgag gccgacgtca ggagttgatg
     3721 tgtcggcggc cagcacgccc tccgcgtgca gcgtccatgc gccggccacg tcgtgttcgt
     3781 cgcggctgtg gatggtcagc gtcccgtcgc cgacggcgat ccgcagctgc aacgcgctgt
     3841 cgtccggtat caccatgggt gcctgaagtg tcagttcccg cacctcgggg agccctgcct
     3901 cgtgtccggc ctgcaacgcc agctccagca gacccgtccc gggtaccagc acggtgccca
     3961 gcacctcgtg gtcggccagc cacggatgcg tggccagaga gatccgcccg gtgatcgtca
     4021 tcccacctgt gtcgggggtc gacaccactg ctccgaccag cgggtgatcg atggtggtca
     4081 acccgacggc cgacacgtca ccgcgagccg ccccggactg gagccagtac cgttcgcgct
     4141 ggaaggcgta ggtcggcaga tcgatgtgtg tcgccacgtc tccgaagtgg ctggtccagt
     4201 cgacgggcac acctgtggtg tgcagccggc cgagcgcttc gatgaacgcc tgggcctcgc
     4261 cgcggtcctt gcgcacggcc ggcacgacca ccgccgcctc gtccagactc tgctgagcca
     4321 gccccgccaa aacaccatca ggcccgatct cgacgaagac cgacgcgtcc gcggcggtga
     4381 caccatcggc gaaccgcaca gcccggcgca cctgatcgac ccagtaaccc gggtcggtga
     4441 gctgcccctc gacaaccaac tgccccgtga cagtggagac gataggcaac acaggctcgt
     4501 gataactgag cgtcgcggcc acagccgcga actcctccaa catcggatcc atcaacggcg
     4561 agtggaacgc atggctgacc cgcaaccgcc gagccc
//
