LOCUS       NZ_QOIM01000040        11198 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Streptomyces reniochalinae strain LHW50302
            NODE_3_length_463606_cov_17.2509_ID_5, whole genome shotgun
            sequence.
ACCESSION   NZ_QOIM01000040
VERSION     NZ_QOIM01000040.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces reniochalinae
  ORGANISM  Streptomyces reniochalinae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 463606)
  AUTHORS   Li,L.
  TITLE     Streptomyces reniochalinae sp. nov. and Streptomyces diacarnus sp.
            nov. from marine sponges
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 463606)
  AUTHORS   Li,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2018) Department of Pharmacy, Ren Ji Hospital
            School of Medicine, Shanghai Jiao Tong University, Pujian, Pudong,
            Shanghai 200127, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-FEB-2016
            Assembly Method                   :: A5-misseq v. MARCH-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003323735.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 01:54:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,751
            CDSs (total)                      :: 6,669
            Genes (coding)                    :: 6,475
            CDSs (with protein)               :: 6,475
            Genes (RNA)                       :: 82
            rRNAs                             :: 6, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 4, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 66 of 194
            Pseudo Genes (incomplete)         :: 154 of 194
            Pseudo Genes (internal stop)      :: 16 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:03:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 164680
            Orig. end                         :: 175878
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QOIM01000040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11198
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11198
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11198
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6198](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6198)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(219..234)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.17"
                     /tool="antismash"
     misc_feature    complement(815..829)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     misc_feature    complement(992..1004)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.65"
                     /tool="antismash"
     gene            999..2459
                     /gene="hemG"
                     /locus_tag="DQ392_RS22565"
                     /old_locus_tag="DQ392_22545"
     CDS             999..2459
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547462.1"
                     /locus_tag="DQ392_RS22565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_22545"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_114017518.1"
                     /sec_met_domain="DAO (E-value: 8.7e-09, bitscore: 29.9,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHVLVIGGGISGLAAAHQLLADGARVTVLEAGAVLGGKLRAGSIA
                     GAAAVDLGAESLLARRPEAVELARAVGLGERLAPPSVAGGALWTRGAVRPMPKGHVMGV
                     PGDPAAVEGVVSPEGLARIEEDERLPRTEVGEDVSVGDYVAGRLGREVVDRLVEPLLGG
                     VYAGDAYRISLRAAVPQLYEAARDERSLLAGVRALQRRAAAAPARTGPVFMGIEGGVGR
                     LPGAVADACTAKGADIRRSTPVRELRRTAGEDGARDWIAVTDHATVRGDAVVVAVPAPA
                     AARLLGPHAPAAAAGLDQVAYASMALVTLAFRRADCGGLPDGSGFLVPPVDGRAIKAAT
                     FSTRKWGWVAEQDPGLFVLRTSLGRHGEERVLERDDAELVELSLADLRAATGLRARPVD
                     TLVTRWRGGLPQYPVGHLERVSQIKDAVARVPGLAVAGAVYEGVGIPACVAAGRRAARH
                     LVQHPANPADPTVPQAGETGEREQRREP"
     gene            2456..3172
                     /gene="hemQ"
                     /locus_tag="DQ392_RS22570"
                     /old_locus_tag="DQ392_22550"
     CDS             2456..3172
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358855.1"
                     /locus_tag="DQ392_RS22570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_22550"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_114017519.1"
                     /transl_table=11
                     /translation="MTDAQPKAPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRAGYAD
                     EVEELFGQLAAEDVVVRGTYDVSGLRADADVMIWWHSEESDALQDAYNRFRRTRLGRAL
                     EPVWSNMALHRPAEFNKSHIPAFLADEQPRDYVSVYPFVRSYEWYLLPDEDRRRMLADH
                     GKMARGFPDVRANTVPAFSLGDYEWVLAFEADELHRIVDLMRHLRGSEARRHVREEIPF
                     YTGRRKPVAELVAGLA"
     gene            complement(3187..4788)
                     /locus_tag="DQ392_RS22575"
                     /old_locus_tag="DQ392_22555"
     CDS             complement(3187..4788)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (101-476). E-value:
                     5.6e-21. Score: 67.2. Matches aSDomain:
                     nrpspksdomains_DQ392_RS22575_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358856.1"
                     /locus_tag="DQ392_RS22575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_22555"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_245981774.1"
                     /sec_met_domain="PF00561 (E-value: 5.2e-26, bitscore: 86.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAVMTATVAALAVAVAPAAPVTAAPAGDVTEPAGVADAAAKAA
                     RAGVEWGTCPKAEHLPDPVECGTVEVPVDYRDPDGERIRLTVSRARATGTPHKRLGPLV
                     YNPGGPGGSGMSFPLYPSLGGLWKRLNERYDFIGYAPRGVGRSGPLSCQAPQEFTKGPN
                     PSPRHPSAAFKEKMKEKAAAYARGCATAQPERLPHYTTANNARDLDVVRAALGQRKLSY
                     LGVSYGTYIGSVYATLFPGHVGRLVLDSVVNPSSDSVWYRANLEQNRAFERRWGDWKSW
                     VARHRDTYHLGATAEEVQRSFDAAMAAVRRKPAGGTVGQKELVAAYLNTGYADALWAPY
                     ARALAAYTAGDDKPLVSLAAPEEAAAKGEENGNAVYNAVECQDAPWPRDWDRWDLDNTA
                     SAAVAPFNTWENAWMNLPCAYWRTRPSHPVEVGTGPGELPKVLLVAATRDAATPYQGAL
                     ETRRRLAGSSLVTEQGAGNHGVSGGNACVDAHLERYLFEGRTPGFHAECAARPEPEPGG
                     RHRAGTKPASNTRAAR"
     aSDomain        complement(3361..4485)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DQ392_RS22575_Abhydrolase_1.1"
                     /evalue="5.60E-21"
                     /label="DQ392_RS22575_Abhydrolase_1.1"
                     /locus_tag="DQ392_RS22575"
                     /protein_end="476"
                     /protein_start="101"
                     /score="67.2"
                     /tool="antismash"
                     /translation="PLVYNPGGPGGSGMSFPLYPSLGGLWKRLNERYDFIGYAPRGVGR
                     SGPLSCQAPQEFTKGPNPSPRHPSAAFKEKMKEKAAAYARGCATAQPERLPHYTTANNA
                     RDLDVVRAALGQRKLSYLGVSYGTYIGSVYATLFPGHVGRLVLDSVVNPSSDSVWYRAN
                     LEQNRAFERRWGDWKSWVARHRDTYHLGATAEEVQRSFDAAMAAVRRKPAGGTVGQKEL
                     VAAYLNTGYADALWAPYARALAAYTAGDDKPLVSLAAPEEAAAKGEENGNAVYNAVECQ
                     DAPWPRDWDRWDLDNTASAAVAPFNTWENAWMNLPCAYWRTRPSHPVEVGTGPGELPKV
                     LLVAATRDAATPYQGALETRRRLAGSSLVTEQGAG"
     gene            complement(5001..6198)
                     /locus_tag="DQ392_RS22580"
                     /old_locus_tag="DQ392_22560"
                     /pseudo=""
     CDS             complement(5001..6198)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675658.1"
                     /locus_tag="DQ392_RS22580"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="DQ392_22560"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 3.3e-94, bitscore: 309.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDRLGTGIGWRPEIAEGVEALPDIDWVEVVAENICPGHLPDSLRR
                     LRARGTVIVPHGVALGLGGAERPDPGRLAALAERAEALGAPLVSEHIAFVRAGGAGTGL
                     PPTEAGHLLPVPRTRDALDVLCENVRIAQDSLPVPLAVENIAALFGWPGEEMSEGAFLS
                     ELVERTGVRLLIDVANLHTNHVNRGEDPCAALDRLPWEALAYVHVAGGVERDGVWHDSH
                     GHPVTRPVLEVLEQLCARAVPPGVLLERDENFPPVARLAAELDAIRAVVEAARV"
     gene            complement(6330..6488)
                     /locus_tag="DQ392_RS34550"
     CDS             complement(6330..6488)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DQ392_RS34550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_181856538.1"
                     /transl_table=11
                     /translation="MAAPAARQAAAFGHTWPLHIVKRVAALVVALAARDGRKERGSAQE
                     RDAAKER"
     gene            complement(6691..7341)
                     /locus_tag="DQ392_RS22585"
                     /old_locus_tag="DQ392_22565"
     CDS             complement(6691..7341)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643872.1"
                     /locus_tag="DQ392_RS22585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_22565"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_245981838.1"
                     /transl_table=11
                     /translation="MPLVVRIERADPPARTDALQTAARAVLALLSDPRTVEETGAWHAR
                     VRQWEERQIRKVVRRARGAEWRRTEPLGGLTVSGHRAQVRVFPPIPLDGWPKELARLQV
                     SGTDLAEPGPLPTPDAGTPVLWLNPGLEMSAGKAMAQAGHGAQLAWWRLEPAARAAWRA
                     SGFPLAVRPARDTAHWHDLTTAGLPLVQDAGFTEVAPGSCTVVAEHPASRTAD"
     gene            7477..8421
                     /locus_tag="DQ392_RS22590"
                     /old_locus_tag="DQ392_22570"
     CDS             7477..8421
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972874.1"
                     /locus_tag="DQ392_RS22590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_22570"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_114017521.1"
                     /transl_table=11
                     /translation="MSWAVTLDDVGRRYRAGGRWVLRGVDLEVRAGTLLRVEGGNGSGK
                     STLLRLLAGVEAPSAGRIRGLPGTRGYVPERFPAALPLTAAAYVTHLGRVRGLRGRAAA
                     RESTRWLERLGAGPYAGTRMRELSKGSSQKVAVAQALVGRPALLVLDEAWTGLDQSARA
                     VLDEVVRERVAEGGAVVFVEHDPARLAAAPDAVVRIAAGGLAERAERGPRARAGTEPRP
                     RTPRLRIEARGTPGAGAPAGLPRGARCRVTPEGVLSLTVPAAASDAALRTLLAAHPGWH
                     VRSVREEDAAREEDAVQERGGPVGPAREEAGPG"
     gene            8418..9095
                     /locus_tag="DQ392_RS22595"
                     /old_locus_tag="DQ392_22575"
     CDS             8418..9095
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016470504.1"
                     /locus_tag="DQ392_RS22595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_22575"
                     /product="ABC transporter"
                     /protein_id="WP_114017522.1"
                     /transl_table=11
                     /translation="MRETLALTRYQLALLVRSQRWLAPLLLYAGLLAVGVRAGDPVLDS
                     LGFAAAALLPTAVWLVRVCVTAEPAAARHCAAAATGPARAHLAGVVAGAAAAAVPGGAA
                     VGLVTAVSSRWSTDGGGAIPVASAVVAGVCGVLTCVAVGTAVGVLCNRPLVGARGYAVA
                     MSLLGTVVALVAGASPARAAVAALSRASAQGSVDVPWVELLCALAVAGLAVCAACAVVG
                     RRP"
     gene            complement(9138..10538)
                     /locus_tag="DQ392_RS22600"
                     /old_locus_tag="DQ392_22580"
     CDS             complement(9138..10538)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602440.1"
                     /locus_tag="DQ392_RS22600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_22580"
                     /product="alkaline phosphatase PhoX"
                     /protein_id="WP_114017523.1"
                     /transl_table=11
                     /translation="MPTTRRLALARTGAVGAAVAFSGALSELFTGTAAAQATGGSAGYG
                     PLVPDPDGLLDLPEGFRYKVLSAEGDRLRSGEGKVPSNCDGMSAFPARPRHGKGHGHGK
                     GHTWLVRNHENRKDSRVPVPTVERLTYDPEGEGGCTVLELDGDNDVLTERVGIAGTSTN
                     CAGGPSLWDTWLTCEETEFKAGEEGYTKDHGFIFEVGFDNRRPTSVEPLKDMGRFQHEA
                     VAFDPHNGVVYETEDAFDAPFGLFYRFLPRKPRGGYGSLHAGGTLQAMRVPGVADLSSV
                     TETGTTFENIEWVEVPDPVAKETPIRFQDFGKGGITHAQKLEGCYWGGSCVYFVSSYAR
                     SSEGSQRDHFGQIWRYDPRRRRLTLVILFGPDTDVQLPGEEPDNICLAPSGGLMVCEDG
                     AGAQHVFGLTRRGTVYPMARGAQNIGTPEEPEWGEFAGVTFAPDGRTMYVNCYTPGTTF
                     AVTGPWRH"
     misc_feature    complement(10617..10631)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.47"
                     /tool="antismash"
     misc_feature    complement(10705..10722)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.59"
                     /tool="antismash"
     misc_feature    10729..10744
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.41"
                     /tool="antismash"
ORIGIN
        1 ccaggtcggt cagcagcccg cccgacgtgc ccaggggcag ctcggcggcg cgcgccagct
       61 ccgtcgccgg ccgtaccccc aggccgagca cgacgacgtc cgccggatac tcggtctcct
      121 cggtcgccac cccgcgggcc cgcccgtcct cgtcggtgag gatctcggtg acggcggctt
      181 cgagcaccgt ctcgatgccc agggagcaca tggcctcgtg gaccagccgg cccatgtcgg
      241 ggtccagggt ggacatgggc tgctcggcct tttcgaggac cgtcacctcg tagccgcgct
      301 ggacgaacgc ctcggccatc tcgacgccga tgtagcccgc gccgacgacg accgctcgcc
      361 gcgcgccgtc accccccgag ccgccgcccc gctcgatgcg ctgttccagt gcgtcgagca
      421 gctggcgtcc gtcgtccagc gtctgcaccc cgtgcacgcc ttcggcgtcg atgccgggca
      481 gcggtgggcg tgtcggcacg gcgccggtgg ccagcaccag gtggtcgtag ccctcccagc
      541 gctccgtgcc ggcctcctcg cgcacccgca cccgcgcccc cgcgaggtcc agctcgaccg
      601 cctcggtgcg catgcgcagg tcgatgccgc gtgagcggtg ctcctcgggg gagcgggcga
      661 tcagcgcgtc ccgctcctgg acgtcgccgc cgacccagta ggggatgccg caggccgagt
      721 acgaggagaa ggggccgcgc tcgaacgcca cgacctccag atcctccggg ctccgtagcc
      781 tgcgggcctg cgacgcggcc gacatgcccg tcgcatcgcc accgatgacc accatccgct
      841 ccgccgccat caccgagctc ctctcgccgt tccgcccgtg cctgccgtgc tgtcccactt
      901 ctacgccggc ttccgcgccc gctgtgccct cccacttctt ctgacccact accgggcccc
      961 acgttatgca ccaatcggca ctctgcgaga cttgcgatat gcacgtactt gtcatcggag
     1021 gtggcatctc gggcctggcc gccgcgcacc aactcctcgc ggacggtgcg cgggtgaccg
     1081 tgctggaggc cggggccgtg ctcggcggca agctgcgtgc cggaagcatc gcaggcgccg
     1141 ccgccgtgga cctgggcgcc gagtccctgc tggcccgccg ccccgaggcc gtggagctgg
     1201 cccgtgcggt cgggctcggc gagcggctgg cgccgccgtc cgtcgcgggc ggcgccctgt
     1261 ggacgcgcgg cgccgtgcgc cccatgccca agggccacgt gatgggcgtg ccgggtgacc
     1321 cggcggccgt ggagggcgtg gtctcgcccg aggggctcgc ccgtatcgag gaggacgagc
     1381 ggctgccgcg taccgaggtc ggcgaggacg tgtctgtggg ggactacgtg gccgggcggc
     1441 tcggccgcga ggtcgtcgac cggctcgtgg aacccctgct gggcggcgtc tacgccggtg
     1501 acgcgtaccg catctccctg cgcgccgcgg ttccgcagct gtacgaggcg gctcgcgacg
     1561 agcgctccct gctggcaggg gtgcgcgccc tccagcggcg ggccgccgcc gcgcccgcgc
     1621 gcacggggcc cgtcttcatg ggtatcgaag gcggcgtcgg ccggctgccc ggggccgtgg
     1681 ccgacgcctg cacggccaag ggcgccgaca tccggcgcag tacaccggtg cgggagctgc
     1741 gccggacggc gggtgaggac ggcgcccggg actggatcgc ggtcaccgac cacgcgacgg
     1801 tgcgcggcga cgcggtcgtg gtggccgtgc ccgcgcccgc ggcggcccgg ctgctgggtc
     1861 cgcacgcgcc ggctgccgcc gccggtctcg accaggtcgc gtacgcctcg atggcgctgg
     1921 tcaccctggc cttccgccgg gccgactgcg gcggtctgcc ggacggcagc ggcttcctgg
     1981 tgccgccggt ggacgggcgc gcgatcaagg ccgccacctt ctccacccgc aagtggggtt
     2041 gggtcgccga gcaggacccc ggcctgttcg tcctgcgcac ctcgctcggc cgccacggtg
     2101 aggagcgggt gctcgagagg gacgacgcgg aactggtcga gctgtccctg gcggacctgc
     2161 gcgccgccac cgggctgcgg gcccgccccg tcgacacgct ggtcacccgc tggcgcggcg
     2221 gcctgccgca gtatccggtg ggccatctgg agcgggtgtc gcagatcaag gacgccgtcg
     2281 cccgggtccc cgggctggcc gtcgccggcg cggtctacga gggcgtgggc atccccgcct
     2341 gtgtcgccgc cggccgccgc gcggcccggc acctcgtgca gcaccccgcc aaccccgccg
     2401 acccgacggt cccccaggca ggggagaccg gcgaacgaga gcagagaaga gagccatgac
     2461 cgacgcacag cccaaggccc ccaacgcggg caagaaggcc aaggacctca acgaggtcat
     2521 ccgctacacc ctctggtccg tcttcaagct gcgcgacgtg ctgcccgagg accgtgccgg
     2581 gtacgcggac gaggtggagg agctgttcgg tcagctcgcc gccgaggacg tcgtggtccg
     2641 cggcacctac gacgtctccg ggctgcgcgc cgacgccgac gtgatgatct ggtggcactc
     2701 ggaggagtcg gacgccctcc aggacgccta caaccgcttc cgccgcacca ggctgggccg
     2761 cgcgctggag cccgtctggt cgaacatggc gctgcaccgt cccgccgagt tcaacaagtc
     2821 gcacatcccg gccttcctcg ccgacgagca gccgcgcgac tacgtgagcg tctacccgtt
     2881 cgtgcgctcc tacgagtggt acctgctgcc cgacgaggac cgccgccgga tgctcgccga
     2941 ccacggcaag atggcgcgcg gcttccccga cgtgcgggcc aacaccgttc cggcgttctc
     3001 gctgggcgac tacgagtggg tcctcgcctt cgaggcggac gaactgcacc gcatcgtcga
     3061 cctcatgcgg cacctgcgcg gctccgaggc gcggcggcac gtccgcgagg agatcccctt
     3121 ctacaccggc cgccgcaagc cggtcgccga gctggtggcc gggctggcgt aggcctcacg
     3181 gcccgctcag cgggcggccc gggtgttgga ggccggcttg gtccccgccc ggtgccgccc
     3241 gccgggctcc ggctcggggc gggctgcgca ctcggcgtgg aacccgggag tcctgccctc
     3301 gaacaggtac cgctccaggt gcgcgtcgac acacgcgttg ccgccgctca cgccgtggtt
     3361 ccccgctccc tgctcggtca ccagcgagga ccccgccagc ctgcgccggg tctccagcgc
     3421 cccctggtac ggggtggccg cgtcccgggt cgcggccacc agcagcacct tcggcagctc
     3481 gccgggcccg gtacccacct ccacggggtg cgagggccgg gttctccagt aggcgcacgg
     3541 caggttcatc caggcgttct cccaggtgtt gaacggggcg acggccgccg aggcggtgtt
     3601 gtccaggtcc cagcggtccc agtcgcgcgg ccagggcgcg tcctggcact cgaccgcgtt
     3661 gtagacggcg tttccgttct cctcgccctt cgccgccgcc tcctcgggcg ccgccaggct
     3721 caccagcggc ttgtcgtccc cggccgtgta cgcggccagt gcccgtgcgt acggtgccca
     3781 cagggcgtcc gcgtatccgg tgttcagata cgcggccacc agctccttct gccccaccgt
     3841 cccgcccgcg ggcttccgcc gcaccgccgc catggccgcg tcgaaggacc gctggacctc
     3901 ctcggccgtc gcccccaggt ggtaggtgtc ccggtgccgc gccacccagc tcttccagtc
     3961 gccccagcgg cgctcgaagg cgcggttctg ctccaggttg gcccggtacc acacgctgtc
     4021 ggacgagggg ttgaccacgc tgtccagcac cagcctgccg acgtgcccgg ggaagagcgt
     4081 cgcgtacacc gagccgatgt aggtcccgta cgagacaccc aggtaactca gcttccgctg
     4141 cccgagcgcg gcgcgcacga cgtccaggtc cctggcgttg ttcgccgtcg tgtagtgcgg
     4201 cagcctctcg ggctgcgccg tggcgcagcc ccgggcgtag gcggccgcct tctccttcat
     4261 cttctccttg aacgcggccg aggggtgccg cggtgacggg ttcgggccct tggtgaactc
     4321 ctggggggcc tggcacgaca aggggccgga acgcccgacg ccgcgcgggg cgtacccgat
     4381 gaagtcgtac cgttcgttca gccgcttcca caggccgccg aggctcgggt agagagggaa
     4441 gctcattccg cttccgccgg gcccgccggg gttgtagacg agtggcccca gccgcttgtg
     4501 cggcgtgccc gtggcccgcg ccctgctgac ggtcagccgg atccgctcac cgtccgggtc
     4561 gcggtagtcg accgggacct cgaccgtccc gcactcgacg gggtcgggca ggtgctccgc
     4621 tttcgggcac gtgccccact cgacccctgc ccgagccgcc ttcgccgcgg cgtcggcgac
     4681 ccccgccggc tccgtcacgt ccccggcggg cgccgccgtc accggtgcgg cgggggccac
     4741 cgccacggcc agcgccgcca ccgtcgccgt catcaccgct gcgcgcactg tgggctccct
     4801 ggcgctggtc gcttgtcgct ccgtcagccg tgccgtgggg caccgctgtt catatcggat
     4861 gctcagccgc tcatacgctc atgtaaagca acgtccccgg gtgtcgccca cgacccctcg
     4921 tacggcccct cgtgcggccc ttgcgtcgta ccgcgccgcg gcgaccgcca cggccccgcc
     4981 ctgaggacac aggcggggcg tcaccgcggg gcgggcccca gccggtggcg cagccaccgc
     5041 ttcagggtcc tgcgctcgcg gcgggcgagc cgtccgcgct ccagcgcgaa gcccgcgaag
     5101 tcgagcgcgt cccaccggtg gttgtcccgc agcggacacc gcgccgcgta cgcgtggaac
     5161 agccgcctga acacgtccgt gcccaggatc tcgggcagct cgggcgccac ctcggcgacg
     5221 atgtcggcac gcttggcccg cagcgcccgc gcctggagct gaagccgctc cgggtcgaac
     5281 cccgccggcg cgggcgcccc cgccaccagg caggcgagca gcccggcctg cgcccgcccc
     5341 agccgctcgc gcgcaccgtc cgtgtgctca gacacgcgcg gcctccacga ccgcgcggat
     5401 cgcgtccagc tcggccgcca accgcgccac gggagggaag ttctcgtcgc gctccagcag
     5461 gacgccgggc ggcaccgccc gggcgcacag ctgttccagc acctccagta ccgggcgggt
     5521 caccgggtgc ccgtggctgt cgtgccagac gccgtcccgc tccacgcctc ccgccacgtg
     5581 cacgtacgcg agtgcttccc agggcagccg gtccagcgcc gcgcacgggt cctccccgcg
     5641 gttgacgtgg ttggtgtgca ggttcgccac gtcgatcagc agccgcaccc cggtgcgctc
     5701 gaccagttcg gacaggaagg cgccctcgct catctcctcg ccgggccagc cgaacagtgc
     5761 ggcgatgttc tccacggcca gcggcaccgg caggctgtcc tgcgcgatgc gcacgttctc
     5821 gcacagtacg tccagcgcgt cccgtgtccg gggaaccggc agcagatgcc cggcctccgt
     5881 cggcggcagg cccgtgccgg cgccgccggc gcggacgaag gcgatgtgct cgctgaccag
     5941 gggagcgccg agcgcctcgg cgcgctcggc gagggcggcg aggcgtcccg ggtcggggcg
     6001 ctcggctccc ccgaggccga gcgccactcc gtgcggcacg atcaccgtgc cgcgcgcccg
     6061 cagccggcgc agggagtccg gcagatgccc tgggcagatg ttctccgcga cgacctcgac
     6121 ccagtcgatg tccggcagcg cctcgacgcc ctcggcgatc tcgggccgcc agccgatccc
     6181 ggtccccagc ctgtccatcc gccgctcctc cccgtggccg cgagtgaact cctgctctgc
     6241 cggcgcccaa cccaaccccc gcagggcctc cttcagagcg aaacttgagg ttcccgacac
     6301 cccgacgtcc ggacgccccg cccccgagct caacgctcct tggcggcatc gcgctcctgg
     6361 gcggatccgc gctccttccg gccgtcgcgt gcggcgaggg cgaccacgag cgcggccacg
     6421 cgcttgacga tgtggagcgg ccaggtgtgt ccgaaggcag cggcctgtcg ggcagccggc
     6481 gcagccacag ttcgcgctcg tgtccgctgc ccgacggcag gtccgcctgg tcgagcagcg
     6541 tcagcacttg ttcgacggcg cgcggcagcc cgcaggtgtg tcatctcccg caccccgatc
     6601 cccctcgcgc gccccccggc gcgcggcgcg ttgcgcccgg tgaggaggat gccaccaacc
     6661 gtggccgcct gtcaggggta cgacaggacg tcagtccgcc gtgcgcgagg cgggatgctc
     6721 cgccacgacg gtgcacgagc cgggcgccac ctcggtgaac ccggcgtcct ggacgagcgg
     6781 gagccccgcg gtggtcaggt cgtgccagtg agcggtgtcg cgtgcagggc gcaccgcgag
     6841 ggggaagccg gacgcgcgcc aggcagcccg cgccgcgggc tcgagccgcc accaggcgag
     6901 ctgcgcgccg tggcccgcct gcgccatcgc cttgccggcg gacatctcca gaccggggtt
     6961 cagccacagc acgggcgtgc cggcgtccgg ggtgggcagt ggcccgggct ccgccaggtc
     7021 ggtgccggag acctggagcc gcgccagctc cttgggccag ccgtccagcg ggatcggagg
     7081 gaagacgcgc acctgggcac ggtggccgct cacggtgagc ccgcccagcg gttccgtgcg
     7141 ccgccactcg gcgccccgcg cccgccgcac caccttgcgg atctgccgct cctcccactg
     7201 ccgcacccgc gcgtgccacg ccccggtctc ctccaccgtg cgcggatcgg acagcagcgc
     7261 caggaccgcg cgcgccgccg tctggagcgc gtccgtgcgc gccggcgggt ccgcccgctc
     7321 gatgcggacg acgaggggca ggacgtagcg gtcgggctcg gcggcgggct gctcgcggga
     7381 ggtggcgggc tccggggccc cggccgcggg ctccggggag gctgaagagg tcgaaacggt
     7441 caccgggtca gtctgccagc ggctagcgtt gcccccatga gctgggctgt cacgctggac
     7501 gacgtggggc gcaggtaccg ggcgggcgga agatgggtcc tgcgcggcgt cgatctggag
     7561 gtgcgcgcgg gcaccctgct gcgcgtcgaa ggcggcaacg gcagcgggaa gtccaccctg
     7621 ctccggctgc tcgccggggt cgaggccccc agcgcgggcc ggatacgggg actgcccggg
     7681 acacgcggat acgtccccga acgcttcccc gccgccctgc cgctgaccgc tgccgcctac
     7741 gtcacccacc tcgggcgcgt gcgtgggctg cgcgggcgtg cggccgcccg ggagagcacg
     7801 cgctggctgg agcgcctggg cgcgggcccg tatgccggca cacgcatgcg ggagctgtcc
     7861 aagggcagca gccagaaggt agccgtggcc caggccctcg tcggccgccc tgccctgctg
     7921 gtgctcgacg aggcgtggac cgggctcgac cagtcggccc gcgcggtcct cgacgaggtg
     7981 gtgcgggagc gggtcgcgga gggcggcgcg gtggtcttcg tggagcacga ccccgctcgg
     8041 ttggcggcgg ctcccgacgc ggtcgtgcgg atcgctgcgg gcggcctggc ggagagggcg
     8101 gagcgcggcc cgcgcgctcg cgcgggtacc gaaccgcgcc cccgaacgcc ccggttgcgc
     8161 atcgaggcgc gggggacgcc gggtgccggt gcgcctgccg ggctgccgcg cggtgcccgc
     8221 tgccgggtga cgcctgaggg cgtgctgtct ttgaccgtgc ccgccgccgc ctcggacgcc
     8281 gccctgcgca ccctgctggc cgcgcacccg ggctggcacg tgcgctccgt acgggaggag
     8341 gacgccgcgc gggaggagga cgccgtccag gagcggggcg gtcccgtcgg ccccgcgcgg
     8401 gaggaggcgg ggccgggatg agggagacac tcgcactgac gcgctaccaa ctcgccctcc
     8461 tggtgcgctc ccagcgctgg ctcgcgccgc tgctgctgta cgcggggctc ctggcggtcg
     8521 gtgtacgcgc gggtgatccg gtgctggact ccctcgggtt cgctgccgcg gcgctgctgc
     8581 ccaccgcggt gtggctggtg cgcgtctgcg tgacggccga gccggccgcg gcccggcact
     8641 gcgccgcggc tgccacggga ccggcccgcg cccacctcgc gggtgtggtg gccggtgcgg
     8701 cggcggccgc cgtgccggga ggtgcggcgg tggggctcgt caccgcggtg agcagccggt
     8761 ggagcacgga cggcgggggc gccatccccg tcgcgtccgc cgtggtggcc ggcgtgtgcg
     8821 gcgtcctcac ctgcgtggcg gtcggaacgg ctgtgggggt gctgtgcaac cggccgctcg
     8881 tcggcgcgcg cggctacgcg gtggccatgt ccctgctggg gacggtcgtg gccctcgtgg
     8941 cgggcgcctc gccggcgcgt gcggcggtcg ccgcgctgtc ccgcgcctcg gcgcaaggaa
     9001 gcgtcgacgt gccgtgggtg gagctgctgt gcgcgctggc ggtggcgggt ctcgccgtgt
     9061 gcgccgcctg cgccgtggtg ggccgccgcc cgtgagagcc cgcccccggg tggaccttcc
     9121 ccgggggcgg gacggtgtca gtgccgccag ggcccggtca ccgcgaaggt ggtgccgggt
     9181 gtgtagcagt tgacgtacat ggtgcgcccg tcgggggcga aggtgacgcc cgcgaactcg
     9241 ccccactccg gttcctcggg ggtgccgatg ttctgggcgc cgcgtgccat cgggtagacg
     9301 gtgccgcgcc gggtgagccc gaagacgtgc tgggcgccgg cgccgtcctc gcacaccatc
     9361 aggcccccgc tgggcgcgag gcagatgttg tcgggctcct cgcccggcag ctgcacgtcg
     9421 gtgtcggggc cgaagaggat gacgagggtg aggcggcggc gccgcgggtc gtaccgccag
     9481 atctgcccga agtggtcgcg ctgcgacccc tcggagctgc gcgcgtagct ggagacgaag
     9541 tagacgcagg agccgcccca gtagcagccc tccagcttct gcgcgtgggt gatgccgccc
     9601 ttgccgaagt cctggaagcg gatgggggtt tccttggcca ccgggtcggg aacctcgacc
     9661 cactcgatgt tctcgaaggt ggtgccggtc tcggtgacgg aggacaggtc ggccacgccg
     9721 ggcacgcgca tggcctgaag ggtgccgccc gcgtgcagcg agccgtagcc gccccggggc
     9781 ttgcggggca ggaagcggta gaacaggccg aagggcgcgt cgaaggcgtc ctccgtctcg
     9841 tagacgacgc cgttgtgcgg gtcgaaggcg acggcctcgt gctggaagcg gcccatgtcc
     9901 ttcagcggct cgaccgaggt ggggcggcgg ttgtcgaagc cgacctcgaa gatgaagccg
     9961 tggtccttgg tgtagccctc ctcgcccgcc ttgaactcgg tctcctcgca ggtgagccag
    10021 gtgtcccaca ggctggggcc gcccgcgcag ttggtcgagg tgcccgcgat gccgacgcgt
    10081 tcggtcagca cgtcgttgtc gccgtccagt tcgaggacgg tgcagccgcc ctcgccctcg
    10141 gggtcgtagg tcagcctttc gacggtgggt acggggacgc gcgagtcctt gcggttctcg
    10201 tggttgcgga cgagccaggt gtggcccttg ccgtgtccgt gtcccttgcc gtgcctgggg
    10261 cgcgcgggga aggcggacat gccgtcgcag ttgctgggca ccttgccctc accggagcgg
    10321 agccggtcgc cctcggcgga caggaccttg tagcggaagc cctcgggcag gtcgagcagt
    10381 ccgtcggggt cggggacgag cgggccgtaa cccgcgctcc cgccggtggc ctgggccgcg
    10441 gcggtccctg tgaacaattc cgagagcgcg ccgctgaagg cgacggccgc cccaacggct
    10501 ccggttcttg ccagcgctag acgtcgtgtc gtgggcatcg ggcactctct cctgttggcg
    10561 gacaggtgac ccgagtgtgt gtaccacgca cgccgacatc cccacaccgc tcgggacgca
    10621 actgattgtc acgcgtccgc gagcgccttg ccgtcccggg cgagtgcggt cagccgggat
    10681 atcgccctga agtacttctt gcggtatccg ccctggagca tgtcctcgct gaacagctgg
    10741 tcgaacggca ccccggcggc gacgacgggc acttcgcggt cgtagagccg gtcggcgagc
    10801 accaccaggc gcagggccgt cgactggtcg ggcaccgggg tcacgtccgt gaggcagacc
    10861 gcgccgacgc ccttgaccag ctcgccgtac cggctggggt ggacccgcga gaggtggccg
    10921 agcagggcgg ggaaggcgtc cagggaggcg ccggcggtcc gctgggcggc gcgggtgacc
    10981 tggtcgtcgg agtacggctc cggcgcctcg ggcagcccgc ggtggcggta gtcctcgccg
    11041 tcgatgcgca gggtgcggaa gtgcgcggac aggccctgga tctcccggag gaagtcggcg
    11101 gccgcgaacc gcccttcgcc gagcttgccg ggcagggtgt tcgacgtggc ggccagcgcc
    11161 acgccgttct ccacgagcct cgccagcagg ctggagac
//
