LOCUS       NZ_QOIM01000038        10405 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Streptomyces reniochalinae strain LHW50302
            NODE_2_length_500062_cov_17.1742_ID_3, whole genome shotgun
            sequence.
ACCESSION   NZ_QOIM01000038
VERSION     NZ_QOIM01000038.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces reniochalinae
  ORGANISM  Streptomyces reniochalinae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 500062)
  AUTHORS   Li,L.
  TITLE     Streptomyces reniochalinae sp. nov. and Streptomyces diacarnus sp.
            nov. from marine sponges
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 500062)
  AUTHORS   Li,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2018) Department of Pharmacy, Ren Ji Hospital
            School of Medicine, Shanghai Jiao Tong University, Pujian, Pudong,
            Shanghai 200127, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-FEB-2016
            Assembly Method                   :: A5-misseq v. MARCH-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003323735.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 01:54:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,751
            CDSs (total)                      :: 6,669
            Genes (coding)                    :: 6,475
            CDSs (with protein)               :: 6,475
            Genes (RNA)                       :: 82
            rRNAs                             :: 6, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 4, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 66 of 194
            Pseudo Genes (incomplete)         :: 154 of 194
            Pseudo Genes (internal stop)      :: 16 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:03:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 57782
            Orig. end                         :: 68187
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QOIM01000038.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    258..272
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     misc_feature    493..507
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: strong, score: 21.15"
                     /tool="antismash"
     gene            complement(1409..2509)
                     /locus_tag="DQ392_RS19785"
                     /old_locus_tag="DQ392_19760"
     CDS             complement(1409..2509)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468153.1"
                     /locus_tag="DQ392_RS19785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_19760"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_114016984.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 2e-20, bitscore:
                     68.0, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSERASLHIKGRVLVGPEDVREQMWVVDGRLTYERPPVAPGATEP
                     TTLNGWVLPGLVDAHCHVGLDAHGPVDDSTSEKQALTDREAGALLLRDAGSPSDTRWID
                     HRDDLPRIIRAGRHIARTRRYIRNYAHEIEPGDLAAYVRREARRGDGWVKIVGDWIDRE
                     EGDLTACWPRWAIEEAIEAAHQEGARVTAHCFAEDSLRDLVEAGIDCVEHATGLTEETV
                     PLFAERGVAIVPTLVNIATFPALADGGEQKFPRWSAHMRQLHARRYETVRAAWDAGIPV
                     FTGTDAGGSLAHGLVGQEVAELVRAGIPARDALAAATWTARDWLGRAGLTEGAPADFVV
                     YDTDPRADVRVLDTPRHVVLRGRVVR"
     misc_feature    2821..2836
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(2928..2943)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.08"
                     /tool="antismash"
     misc_feature    complement(3032..3046)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.04"
                     /tool="antismash"
     gene            3044..3559
                     /gene="ectA"
                     /locus_tag="DQ392_RS19790"
                     /old_locus_tag="DQ392_19765"
     CDS             3044..3559
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358302.1"
                     /locus_tag="DQ392_RS19790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_19765"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_114016985.1"
                     /transl_table=11
                     /translation="MPEGLKLDTPRVDDGAAIWRIARDSKTLDLNSSYSYLLWCRDFAA
                     TSVVARDGAGEPVGFITGYVRPEAPRTLLIWQVAVDEAARGRGLAAAMLDGLTLRAAEE
                     CGIDSIETTVTPDNTPSNRLFTSYAQRHSAAVAKDVLFDAALFPEDGHEPEVGYRIGPV
                     AFPGASKR"
     gene            3654..4922
                     /gene="ectB"
                     /locus_tag="DQ392_RS19795"
                     /old_locus_tag="DQ392_19770"
     CDS             3654..4922
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-365). E-value: 1e-75.
                     Score: 246.8. Matches aSDomain:
                     nrpspksdomains_DQ392_RS19795_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="DQ392_RS19795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_19770"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_114016986.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.6e-80, bitscore:
                     264.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTIFDRAQGAHMYDEDGHTY
                     LDFFAGAGTLNYGHNNPVLKRALLDYLERDGVVHGLDMSTSAKRSFLETFQEVILQPRG
                     LDHKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDDYLNGTTEDFVWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLKALSELCERHDMLLIVDDIQMGCGRTGAFFSFEEAGIKPDIITLSKSIGGYGMPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTATAALQTYWADGQMEKQTLSRGEQVEAALK
                     AIAEEHAGEVADHRGRGLVWGLEFHNKSRANAVARRAFELGLLIETSGPQSEVVKLLPS
                     LTITPEELDEGLRILARAVRETV"
     aSDomain        3741..4748
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DQ392_RS19795_Aminotran_3.1"
                     /evalue="1.00E-75"
                     /label="DQ392_RS19795_Aminotran_3.1"
                     /locus_tag="DQ392_RS19795"
                     /protein_end="365"
                     /protein_start="29"
                     /score="246.8"
                     /tool="antismash"
                     /translation="DRAQGAHMYDEDGHTYLDFFAGAGTLNYGHNNPVLKRALLDYLER
                     DGVVHGLDMSTSAKRSFLETFQEVILQPRGLDHKVMFPGPTGTNAVEAALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDDYLNGTTEDFVWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARAEWLKALSELCERHDMLLIVDDIQMGCGRT
                     GAFFSFEEAGIKPDIITLSKSIGGYGMPLALTLFKPELDIWEPGEHNGTFRGNNPAFVT
                     ATAALQTYWADGQMEKQTLSRGEQVEAALKAIAEEHAGEVADHRGRGLVWGLEFH"
     gene            5001..5405
                     /locus_tag="DQ392_RS19800"
                     /old_locus_tag="DQ392_19775"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141824.1"
                     /locus_tag="DQ392_RS19800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_19775"
                     /product="ectoine synthase"
                     /protein_id="WP_114016987.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.6e-55, bitscore:
                     179.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTDRHIKSQSGTWESKRIVLAKEKVGFSLHETILY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKHWISPGTMYLLNGHEKHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPDES"
     gene            5542..6441
                     /gene="thpD"
                     /locus_tag="DQ392_RS19805"
                     /old_locus_tag="DQ392_19780"
     CDS             5542..6441
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="DQ392_RS19805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_19780"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_114016988.1"
                     /sec_met_domain="PhyH (E-value: 1.5e-40, bitscore: 134.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGATEVATPRMDPVVWSEPGTPGPIEATDLA
                     GFERDGFLAIDQLITSDEVETLRAELERLTSDSEIRADERSIVEPKSSEIRTVFEVHKI
                     SEVFAQLVRDPRIVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHAEDGLA
                     NMRTVSVSIALTENYDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGIPSDES
                     LTKFADAHGIKLFTGKAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENEAVEPFA
                     APVRRPEFIGARDFTPVK"
     gene            complement(6556..7620)
                     /locus_tag="DQ392_RS19810"
                     /old_locus_tag="DQ392_19785"
     CDS             complement(6556..7620)
                     /NRPS_PKS="Domain: Aminotran_5 (15-348). E-value: 1.7e-22.
                     Score: 71.8. Matches aSDomain:
                     nrpspksdomains_DQ392_RS19810_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468158.1"
                     /locus_tag="DQ392_RS19810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_19785"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_114017298.1"
                     /sec_met_domain="Aminotran_5 (E-value: 5.6e-17, bitscore:
                     56.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDERLLAAEFAPEVCYLNTASHGLLPARTVAAVAQAATRMAAGHI
                     DQAAYYAEADAARGAFARLVGTTPDRVAAGSAVSTHVGLIAASLPAGARVLAPENEFSS
                     VVNPFTVREDVQLRTVPLEALADAVDDTTTLVALSAVQSVDGRIADLPAVREAARAAGA
                     RVMLDVTQAAGWLPLSADDYDYVVCGAYKWLLCPRGTSFLVVSRQAQEAGALRPLHAGW
                     VAGASPWDDCYGPVARLAPHARAYEQPVPFLSYVGAARSLALIEELGGPEAIGAHDRAL
                     AARFRAGVAELDGGYAPVPGDSAVVAVPGLGHAAERLAAEGVSLSVREGNLRAGFHLYN
                     TGDDVDRLLGLLKG"
     aSDomain        complement(6577..7575)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DQ392_RS19810_Aminotran_5.1"
                     /evalue="1.70E-22"
                     /label="DQ392_RS19810_Aminotran_5.1"
                     /locus_tag="DQ392_RS19810"
                     /protein_end="348"
                     /protein_start="15"
                     /score="71.8"
                     /tool="antismash"
                     /translation="YLNTASHGLLPARTVAAVAQAATRMAAGHIDQAAYYAEADAARGA
                     FARLVGTTPDRVAAGSAVSTHVGLIAASLPAGARVLAPENEFSSVVNPFTVREDVQLRT
                     VPLEALADAVDDTTTLVALSAVQSVDGRIADLPAVREAARAAGARVMLDVTQAAGWLPL
                     SADDYDYVVCGAYKWLLCPRGTSFLVVSRQAQEAGALRPLHAGWVAGASPWDDCYGPVA
                     RLAPHARAYEQPVPFLSYVGAARSLALIEELGGPEAIGAHDRALAARFRAGVAELDGGY
                     APVPGDSAVVAVPGLGHAAERLAAEGVSLSVREGNLRAGFHLYNTGDDVDRL"
     gene            complement(7694..9229)
                     /locus_tag="DQ392_RS19815"
                     /old_locus_tag="DQ392_19790"
     CDS             complement(7694..9229)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263415.1"
                     /locus_tag="DQ392_RS19815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_19790"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_245981685.1"
                     /sec_met_domain="Aldedh (E-value: 6.1e-45, bitscore: 148.3,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGAVPVWSVDPRTGNQRERVAEEATAADVDAAVRAARDALPSLA
                     DRGARAAFLRTAAAHLEADGERIVATADAESALGAPRLTGELARTTGQLRAFADEVEDG
                     DHLGVVIDHADPSRTPPQPDLRRYKLPLGVVAVYAASNFPLAFSVPGGDTASALAAGCP
                     VVVKAHPDHPATSQLCAAALRSAAVEAGLAPDVVSVVHGFDAGVELVRHPLVSAAGFTG
                     SVRGGRALFDAASSRPVPIPFHGELGSLNPVVLTPSAVAERAEQLGQGLGGSVTLGTGQ
                     FCVKPGLVLVPEGPDGDRTLDALAATLRDTAPGHLLDARMRDAFLDGVRARAALAKVSA
                     PVEAGAGAEGTGRTVRPGVLTLAARDLDEGHALLWEECFGPVTVAVRYGDESDIEAVLA
                     RVTGSLTATVHLGTEEAAQEGGTAAALLARLTALAGRVVVNGWPTGVAVAPAQHHGGPY
                     PATTSTSTSVGATAVERWLRPVAYQDTPEALLPRELRDGNPLGLPRRVDGTRE"
     misc_feature    complement(9377..9395)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.99"
                     /tool="antismash"
     gene            9409..10179
                     /locus_tag="DQ392_RS19820"
                     /old_locus_tag="DQ392_19795"
     CDS             9409..10179
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761328.1"
                     /locus_tag="DQ392_RS19820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_19795"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_114016990.1"
                     /transl_table=11
                     /translation="MSATEAGSAQVKSAVRTVQLLEFFAGRPGMHTLAGVQEALGYPKS
                     SLYMLLRTLVDLGWVEADATGTRYGIGVRALLVGTSYLDGDEVVAAAKPTLDRLAAQTT
                     ETIHLARLDGTDVVYLATRASQHHLRPFSRAGRRLPAHSTSLGKALLATHTDAEVRTLL
                     PARLARVTDNTLTDPEELLEELRLVREQGYAIDREENTLGLRCFGIAVPYRSPARDAIS
                     CSVPVARLTPAHEQAVKSALFDARDQLMQAARTL"
ORIGIN
        1 tgggggtgcc gcactgggcc gcgtaggcga cggcccggcc ttcgtgcaac ccgaacccgc
       61 acacgacccc cgggtccacg cccccgcagc ggtcgcccct gatctccgta cggctgctga
      121 agtaggcgtc gagatacgcg ggggcccggg ggcgctcggc ggcgcgggcc cgggcgacgg
      181 ccgccgggcc gctgccgggc ggtccgccgg gccgtcccgc gtcggggccc gcttggggct
      241 ccgacgcggt gccgcgcggg ccgaccggct ccggtgtgtc cgggcggggg cgcgcgccgg
      301 ctcgctcctc gcccgcgggg ggagcggccc gcgtcccgcg cgggggcggt gccggcaggg
      361 cgtacggcac cggcgcgggc tccggaggcg aggtacccgc tccccggggg ccggcggagt
      421 caccgggccg ggcgccgccg cccgtgagca gggtgagcca gtgcgcgagg acgggggcga
      481 gtccggcttc ggggacgatg tggtccaggt gcccggccgc gtgctggccc tccacggtgt
      541 acgcggcagg gtcggcgtcg cgggggcgta cgcgtgatcc ggcgaatgcg gcctgtgcac
      601 cgggcaggcc gaggaccacg tcggcgcccg cggcgagggt cgcccaccct ccgccggtcg
      661 tcgggtcgcg gacgacggcc agctgcggca gcccggcggc gcgggtcagc gcggtctgcc
      721 gggccacgcg ctggagctgg acgagcgcgc gcatgccctc ctgcatgcgg ctgccgccgg
      781 tggcgacgag ggtgacgacg ggcagcctca gctgccgggc ccggcggtgg gcggcctcga
      841 cgcggtcgcc ggtgcgctgc ccgagcgagc cgccgaggta gccgaactcg aagcacacga
      901 ggacggtttc ggtgccgccg atcctgcccg tgccgcacac cacggactcg tcctccccgg
      961 tgcgttcgcg cgcccgggcc ctcgcccggt cgtacccggg ccaccccagc ggcccgtcca
     1021 cgggctcccg ggccgccgac tgtgcgtccg tcgactcccc gttcgtcggc tggagagccg
     1081 acggctcatc gccgaccggc ccttcgtcca ccggctcccc ggcggcccgc cacgcggcct
     1141 ccccgccctg gccccggccg cctcccgcgg tggcgcaggc aggcgtcggc gcgggtccga
     1201 actcgcgaaa gccgtccgtc aaccggtcga gcagcgctcg cgcgccggtc tgggaggggc
     1261 cgcggcgctc gggggcgtgc gtgtcgcgcg gcgcgccggc gtcgccgccc tcgtccggtg
     1321 cgctgtcacc gggtgccatg gggctcatgc gtccagcgtg cggcaccggg tgccgcttgt
     1381 cagcacggca cctcgccgag ggcgtgcctc agcgcaccac ccgacccctg agcaccacgt
     1441 ggcgcggcgt gtccaggacg cggacgtccg cgcgcggatc ggtgtcgtag acgacgaagt
     1501 cggcgggggc cccctcggtc agcccggccc tgcccagcca gtcgcgggcc gtccaggtgg
     1561 cggcggccag cgcgtcgcgg gcggggatac cggccctgac gagttcggcg acctcctggc
     1621 cgacgagccc gtgtgcgagg gagccgcccg cgtcggttcc ggtgaagacg gggatgccgg
     1681 cgtcccaggc ggcgcgcacg gtctcgtagc ggcgggcgtg cagctgacgc atgtgggccg
     1741 accagcgggg gaacttctgc tccccgccgt cggcgagggc gggaaaggtg gcgatgttga
     1801 cgagggtggg cacgatggcg acgccgcgct cggcgaagag cgggacggtc tcctcggtga
     1861 gcccggtggc gtgctcgacg cagtcgatgc ccgcctccac caggtcgcgc agcgagtcct
     1921 cggcgaagca gtgtgcggtg acgcgggcgc cctcctggtg ggccgcttcg atggcctcct
     1981 cgatggccca gcgcggccag caggcggtga ggtcgccctc ttccctgtcg atccagtcgc
     2041 cgacgatctt gacccagccg tccccgcgcc gggcctcgcg gcgtacgtag gcggccaggt
     2101 cgccgggttc gatctcgtgc gcgtagttgc ggatgtagcg gcgggtgcgg gcgatgtgcc
     2161 gcccggcgcg gatgatgcgg ggcaggtcgt cgcggtggtc gatccagcgc gtgtcggagg
     2221 gcgagccggc gtcgcggagc agcagcgcgc cggcctcgcg gtcggtgagc gcctgcttct
     2281 cgctggtgga gtcgtcgacg gggccgtgcg cgtcgagccc gacgtggcag tgggcgtcca
     2341 cgagcccggg cagtacccag ccgttcaggg tggtcggctc ggtggcaccg ggggccaccg
     2401 gtggccgttc gtaggtgagg cgcccgtcca ccacccacat ctgctctcgt acgtcctcgg
     2461 gcccgaccag tacccgcccc ttgatgtgca gactcgcgcg ctcgctcatg gcggcactgt
     2521 acgagtggcc ggtacgcggg tgtccagggt gcccagggcg gccggtccgg tgcggattcc
     2581 ccgcgcggcg cgcggctcat cgggaacgga agggggccgg ggagaagccg ggaagggacc
     2641 acggagcgac atcgcggaaa acggagacag gtgcttgctc cccggtttac gatgcaatac
     2701 cggcgtcttt tcgcgtacag gttcccgtct tcttctgccc gctttccccg gtctcaaacg
     2761 caaagatttc gctggtgccg aagcacgtat tccgggccac tctccctacc gttatcgcac
     2821 tgtgacgtac tccacaggtc ggctgctata tcccgacata gcgcaccaaa aagggggcgt
     2881 gaacggcgag gcagcgcgcc cgcacgcccg cgcgcgataa catgaagcac ccccctcggg
     2941 taacccctga cccccgtgca ctttcggatt ctgctcggta aattgaattc gcatgaccgc
     3001 cgcacaagca gaccatgcac gtgcccacag agactttctg gaaatgccgg agggactgaa
     3061 gctcgacaca ccgcgtgtgg acgacggagc cgcgatctgg cgcatcgccc gcgactcgaa
     3121 gaccctcgat ctcaactcct cctacagcta cctgctgtgg tgccgcgact tcgccgccac
     3181 ctcggtcgtc gcccgcgacg gagcgggtga accggtcggc ttcatcaccg gctacgtccg
     3241 gcccgaggcg ccgcgcaccc tgctgatctg gcaggtggcc gtcgacgagg cagcccgcgg
     3301 ccgcgggctc gcggccgcga tgctggacgg actgacactc cgtgccgccg aggagtgcgg
     3361 catcgacagc atcgagacca cggtcacccc cgacaacacc ccctccaacc ggctgttcac
     3421 ctcctacgcg cagcgccact cggcagcggt cgccaaggac gtgctgttcg acgccgccct
     3481 cttccccgag gacggccacg agcccgaggt gggctaccgg atcggtccgg tcgcgtttcc
     3541 cggcgcatcg aagcgctgac gtatacggcc aggccagagc gggtaggccc gtcgccggcc
     3601 cgccctaccg acagactttc cttcccccgc gactcactcc ccaggagcaa gctgtgacca
     3661 tcacccagcc ggacctcagc gtcttcgaaa ccgtggagtc ggaggtgcgc agctactgcc
     3721 gtggctggcc gaccatcttc gaccgggcgc agggcgcgca catgtacgac gaggacggcc
     3781 acacctacct cgacttcttc gccggagcgg gcaccctcaa ctacggccac aacaacccgg
     3841 ttctcaaacg ggcccttctg gactacctgg agcgcgacgg cgtcgtgcac gggctggaca
     3901 tgtccacctc ggccaagcgc tccttcctgg agaccttcca ggaggtgatc ctccagccgc
     3961 gcggcctcga ccacaaggtg atgttccccg gcccgacggg caccaacgcc gtggaagcgg
     4021 cgctgaagct ggcccgcaag gtcaagggcc gcgagtcgat cgtctcgttc accaacgcct
     4081 tccacggaat gtcgctcggc tcgctggccg tgaccggcaa cgcgttcaag cgcgcgggcg
     4141 ccggtatccc gctggtgcac ggcacaccga tgccgttcga cgactacctc aacggcacca
     4201 ccgaggactt cgtctggttc gagcggctgc tggaggacca gggctcgggg ctgaacaagc
     4261 cggccgccgt gatcgtggag accgtgcagg gcgagggcgg catcaacgtc gcccgcgccg
     4321 agtggctcaa ggcgctctcc gagctgtgcg agcggcacga catgctgctg atcgtcgacg
     4381 acatccagat gggctgcggc cgcaccggcg ccttcttctc gttcgaggag gcgggcatca
     4441 agccggacat catcacgctg tcgaagtcca tcggcgggta cgggatgccg ctggcgctca
     4501 ccctgttcaa gccggagctg gacatctggg agccgggcga gcacaacggc accttccgcg
     4561 gcaacaaccc cgccttcgtc accgcgaccg ccgcgctaca gacctactgg gccgacggac
     4621 agatggagaa gcagacgctc tcgcgcggtg agcaggtcga ggcggcgctc aaggccatcg
     4681 ccgaggaaca cgccggtgag gtcgccgacc accgcggccg cggcctggtg tggggcctgg
     4741 agttccacaa caagagccgg gcgaacgccg tggcccgtcg cgccttcgag ctggggctgc
     4801 tcatcgagac ctcgggcccg cagagcgagg tcgtcaagct gctgccctca ctgaccatca
     4861 cccccgagga actggacgag gggctgcgca tcctcgcgcg agccgtacgc gagacggtct
     4921 gaccccccgg acgcggcccg tcccggcaca accgggacgg gcctcttccg tgatacacac
     4981 agagaaaggc accatcagac gtgatagtcc gttcattcaa ggacatcgag aacaccgacc
     5041 gtcacatcaa gtcacagtcg ggcacttggg agagcaagcg catcgtcctg gcgaaggaaa
     5101 aggtgggctt ctccctgcac gagacgatcc tctacgcggg gacggagacg tcgatgtggt
     5161 acgccaacca catcgaggcc gtgctctgcg tcgagggcga ggcggaactg accaacgacg
     5221 agaccggcga gaagcactgg atctctcccg gcacgatgta cctgctcaac gggcacgaga
     5281 agcacacgat gcgcccgaag accgacttcc gctgcgtgtg cgtcttcaac ccgccggtca
     5341 ccggccgtga ggaccacgac gagaacggcg tctacccgct gctcaccgag ccggacgaga
     5401 gctgagcacc aagctcccgc cgcgaaaagg ggaccgttcg ccggtccacg tcggccggac
     5461 ggcctcccgg caccacgggg aggccgtccg gaccgtttcg cctgagaaca gggccgtacg
     5521 agaggagagg aaggcaccac catgaccacc gcacccgagc gcaccgccga cctgtacccg
     5581 acccgtggag ccaccgaggt ggccactccg cggatggacc ccgtcgtgtg gtccgaaccg
     5641 ggtactccgg ggcccatcga ggccacggac ctggcgggct tcgagcggga cgggttcctc
     5701 gccatcgacc agctcatcac ctccgacgag gtggagacgc tgcgcgcgga actggaacgg
     5761 ctcacctcgg attcggagat ccgcgcggac gaacgctcga tcgtcgagcc gaagtcgtcg
     5821 gagatccgga ccgtgttcga ggtgcacaag atcagcgagg tcttcgccca gttggtgcgg
     5881 gacccgcgca tcgtgggcag ggcacggcag atcctgggct cggacgtcta cgtccaccag
     5941 tcccggatca acgtcaagcc gggcttcggg gccacgggct tctactggca ctccgacttc
     6001 gagacctggc acgcggagga cggtctggcg aacatgcgga ccgtgtcggt ctcgatcgca
     6061 ctgacggaga actacgacac caacggcggc ttgatgatca tgccggggtc gcacaagacc
     6121 ttcctcggct gcgcgggcga gacgccgaag gacaactaca agaagtcgct gcagatgcag
     6181 gacgcgggga ttccgtccga cgagtccctg accaagttcg ccgacgcgca cggcatcaaa
     6241 ctgttcaccg gcaaggccgg ctcggcgacc tggttcgact gcaactgcat gcacggaagc
     6301 ggtgacaaca tcactcccta cccgcggagc aacgtgttca tcgtcttcaa cagcgtcgag
     6361 aacgaggcgg tggagccctt cgccgcgccc gtccgccgcc ccgagttcat cggggcccgg
     6421 gacttcacgc ccgtgaagtg acccggcagg cggcccgctg acgtcggggc gggacagtgc
     6481 acggtcccgc cccttcgccg agcccccgca cggggcggcg ctccccggcc caccaccggt
     6541 gcgccggtgc cgcgctcagc ccttcagcag ccccagcagc cggtccacgt cgtccccggt
     6601 gttgtacagg tggaagccgg cgcgcagatt gccctcacgg accgagagtg acaccccctc
     6661 ggccgccagc cgctccgcgg catgaccgag ccccggcacg gcgacgaccg ccgagtcccc
     6721 cggcaccggt gcgtacccgc cgtccaactc ggcgacccct gcgcggaacc gcgccgcgag
     6781 cgcccggtcg tgcgcgccga tggcctcggg cccgccgagc tcctcgatca gcgccagcga
     6841 ccgcgcggcc cccacgtacg acaggaacgg caccggctgt tcgtacgccc gcgcgtgcgg
     6901 cgcgagccgg gcgacgggcc cgtagcagtc gtcccacggc gaggcccccg cgacccatcc
     6961 ggcgtgcagc ggccgcagcg cccccgcctc ctgcgcctgg cgtgagacga ccaggaacga
     7021 ggtgccccgc gggcagagca gccacttgta ggcgccgcac accacgtagt cgtagtcgtc
     7081 ggccgacagt ggcagccagc ccgccgcctg cgtcacgtcc agcatcacgc gggcaccggc
     7141 ggcgcgtgcg gcctccctga ccgccggcag atcggcgatc cttccgtcga ccgactgcac
     7201 cgcgctcaac gccaccagcg tcgtcgtgtc gtccaccgcg tcggccagcg cctcgagcgg
     7261 aacggtccgc aactggacgt cctcacggac ggtgaagggg ttcacgacgg aggagaactc
     7321 gttctcgggc gcgaggaccc gcgcgccggc agggagcgag gccgctatca gcccgacgtg
     7381 cgtggagacc gcgctgcccg ccgccacgcg gtcgggcgtc gtgcccacga ggcgcgcgaa
     7441 cgccccgcgc gccgcgtccg cctcggcgta gtacgcggcc tggtcgatgt ggcccgcagc
     7501 catacgcgtg gcggcttgtg cgaccgccgc aaccgtgcgg gcggggagca gaccgtggga
     7561 ggcggtgttg aggtagcaga cctcgggcgc gaactcggcg gcaagcaggc gttcatccat
     7621 ccgcccagtc tcgccgggcg cggaggccgg gtccaccggg ttgtttcccc ggcttcccgg
     7681 cccgcccgct gcctcactcc cgggtgccgt ccacccgccg cggcagtccc agcgggttcc
     7741 cgtcccgcag ttcccgtggc agcagcgctt caggggtgtc ctggtaggcg acgggccgca
     7801 gccagcgttc gacggcggtg gcgcccacgg aggtggaggt ggaggtcgtg gccgggtagg
     7861 gccctccgtg gtgctgggcg ggcgcgacgg ccaccccggt gggccacccg ttcaccacca
     7921 cccgccccgc gagggcggtg agccgggcga gcagggcggc ggcggtgccc ccttcctgcg
     7981 cggcctcctc ggtgccgagg tgcacggtgg cggtgagcga cccggtcacc cgggcgagca
     8041 cggcctcgat gtccgactcg tcgccgtagc gcacggcgac ggtcacgggc ccgaagcact
     8101 cctcccacag cagtgcgtgc ccctcgtcca ggtcgcgtgc ggcgagggtg agcacgccgg
     8161 gccgcacggt gcgtcccgtc ccctccgcgc ccgctccggc ctcgacagga gccgacacct
     8221 tggcgagggc agcccgtgcg cggacaccgt cgaggaaggc gtcccgcatc cgcgcgtcga
     8281 gcagatgtcc gggcgcggta tcgcgcagcg tcgcggcgag cgcgtcgagc gtccggtcgc
     8341 cgtcggggcc ttcggggacg aggacgagtc cgggcttgac gcagaactgt ccggtgccca
     8401 gtgtcacgga gccgccgagc ccctggccga gctgttcggc gcgttcggcg acggcggagg
     8461 gggtgaggac gacggggttg agggagccga gttcgccgtg gaaggggatg ggcacggggc
     8521 gggaggacgc ggcgtcgaag agggcgcgtc cgccgcgtac cgatccggtg aacccggcgg
     8581 cggagaccaa cgggtggcgc accagttcga cgccggcgtc gaatccgtgc accaccgaga
     8641 cgacgtccgg cgcgagaccc gcctcgaccg cggccgaacg cagcgcggcg gcgcacagtt
     8701 gagaggtcgc ggggtggtcg gggtgcgcct tgacgacgac ggggcagccg gccgcgaggg
     8761 cgctggcggt gtcgcctccg gggacggaga aggcgagcgg gaagttggag gcggcgtaga
     8821 cggcgacgac cccgagcggc agcttgtagc ggcgcaggtc gggctgcggc ggggtgcgcg
     8881 agggatcggc gtggtcgatg acgacgccga ggtggtcgcc gtcctcgacc tcgtcggcga
     8941 aggcgcgcag ctgccccgtg gtgcgggcga gttcgccggt gagccggggc gcgccgagcg
     9001 cgctctcggc gtcggcggtc gccacgatgc gctcgccgtc ggcctccagg tgcgcggccg
     9061 cggtccgcag gaaggccgcc cgcgcgccgc ggtcggcgag cgagggcagc gcgtcgcgtg
     9121 ccgcccgcac ggcggcgtct acgtcggccg cggtggcctc ctccgcgacg cgctcccgct
     9181 ggttcccggt gcgggggtcg acgctccaga ccggtactgc tcctgccact gcggccacct
     9241 gtgtcccttc gcgttccgcg gatgcgcccg tgccgtcgtc cgttccgccg gaccggtccc
     9301 gcggatccgc gctcagacag gccatcttgt tcggtattct gaacaacgtc cctgtaggtg
     9361 aatacccttc gggactgtag gtccgcctga actaaggggt caaggtcgat gtccgctacc
     9421 gaggcgggga gcgcgcaggt caagtcagcc gtccggacgg tgcaactgct ggagttcttc
     9481 gccggccgcc cgggcatgca cacgctcgcc ggcgtccagg aagcactcgg ctaccccaag
     9541 tcgagcctct acatgctgct gcgcacactc gtcgacctcg gctgggtgga ggccgacgcg
     9601 acgggcaccc gctacgggat cggcgtccgc gccctgctgg tgggcacctc ctacctggac
     9661 ggcgacgagg tggtggcggc cgcgaaaccc accctcgaca ggctcgccgc acagaccacc
     9721 gagaccatcc acctggcccg gctggacggc accgacgtcg tctacctcgc gaccagggcc
     9781 tcgcagcacc acctgcgccc cttctcccgg gccggacggc ggctccccgc gcactccacc
     9841 tcgctcggca aggcgctgct ggccacccac acggacgcgg aggtgcgcac gctgctgccc
     9901 gcacgcctag cgcgcgtcac cgacaacacc ctcaccgacc cggaagaact gctggaggag
     9961 ctgcggctgg tgcgcgagca gggctacgcg atcgaccggg aggagaacac cctgggcctg
    10021 cgctgtttcg gcatcgccgt gccctaccgc tcccccgccc gcgacgcgat cagctgctct
    10081 gttccggtgg cccggctgac accggcgcac gaacaggcgg tgaagagcgc gctgttcgac
    10141 gcgcgggatc aactgatgca ggccgcccgc acgctctgac ggctatggct acggctacga
    10201 ctgccgctac cgcccgtacg aggtgacggc tgcgtccgca cacctgcggc ccgcggggag
    10261 gacggcggct ccgccgtgcg gcggagccgg ttcgtcgtcc agcaggacgt tcccccggca
    10321 ccggccagga acagtgaagg gcatggagag agccagaacc gtcctcgtcg cactgaccgt
    10381 cgccgcgtgc ctgccgtacc tcacc
//
