LOCUS       NZ_KZ845884            10216 bp    DNA     linear   CON 11-APR-2024
DEFINITION  Enterococcus faecalis strain 262EA1 aekev-supercont1.4, whole genome
            shotgun sequence.
ACCESSION   NZ_KZ845884
VERSION     NZ_KZ845884.1
KEYWORDS    WGS; RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49509)
  AUTHORS   Earl,A.M., Van Tyne,D., Lebreton,F., Saavedra,J.T., Gilmore,M.S.,
            Manson Mcguire,A., Clock,S., Crupain,M., Rangan,U., Young,S.,
            Abouelleil,A., Cao,P., Chapman,S.B., Griggs,A., Priest,M., Shea,T.,
            Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genomics Platform, The Broad Institute Genome
            Sequencing Center for Infectious Disease
  TITLE     The Genome Sequence of Enterococcus faecalis 262EA1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 49509)
  AUTHORS   Earl,A.M., Van Tyne,D., Lebreton,F., Saavedra,J.T., Gilmore,M.S.,
            Manson Mcguire,A., Clock,S., Crupain,M., Rangan,U., Young,S.,
            Abouelleil,A., Cao,P., Chapman,S.B., Griggs,A., Priest,M., Shea,T.,
            Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genomics Platform, The Broad Institute Genome
            Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUN-2015) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R48559
            Genome Coverage                   :: 131.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003319555.1-RS_2024_04_11
            Annotation Date                   :: 04/11/2024 13:29:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,976
            CDSs (total)                      :: 2,907
            Genes (coding)                    :: 2,847
            CDSs (with protein)               :: 2,847
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 5, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 4, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 25 of 60
            Pseudo Genes (internal stop)      :: 25 of 60
            Pseudo Genes (multiple problems)  :: 25 of 60
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:38:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 35630
            Orig. end                         :: 45846
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ845884.1.
            Please be aware that the annotation is done automatically with
            little or no manual curation.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(195..977)
                     /locus_tag="EB41_RS15515"
                     /old_locus_tag="EB41_02936"
     CDS             complement(195..977)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1157: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369746.1"
                     /locus_tag="EB41_RS15515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02936"
                     /product="ParA family protein"
                     /protein_id="WP_010709173.1"
                     /transl_table=11
                     /translation="MARIISVANQKGGVSKTTTSINLGAALHFTFNKRVLVIDMDPQGN
                     ATDNFGFDIDGTNIPTIYEVLKDEASITESILNYKGIEVIPADIALSSAEREFTQVGSE
                     HRLKRVLQPIEENYDYIIIDCPPSLGILTVNAFTVSDEIIIPVEAAYFSLKGLVKLNET
                     IETVKEYTNENLIIRGVLFTKYNERFNISKEMNKTANQISDALHVNIFDTYIRRTITVD
                     EAQAAGSDLISFNKTSTAEDDYIQFTKEFLEKVGDLNE"
     gene            complement(1202..1492)
                     /locus_tag="EB41_RS15520"
                     /old_locus_tag="EB41_02937"
     CDS             complement(1202..1492)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632434.1"
                     /locus_tag="EB41_RS15520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02937"
                     /product="hypothetical protein"
                     /protein_id="WP_010709172.1"
                     /transl_table=11
                     /translation="MPRKTFVYPHPINTYIIKQLGITVEEFCELHAFSQGTVSSWITRN
                     KKIETLPISFIYSLSLSASKTMDQVYSDLLKLQDDYLLHLERHRRTKKIIE"
     gene            complement(1743..1829)
                     /locus_tag="EB41_RS15525"
     CDS             complement(1743..1829)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /locus_tag="EB41_RS15525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196942.1"
                     /transl_table=11
                     /translation="MFEKLISLVIAPLLVGLVLLLVDRWLDD"
     misc_feature    1784..1798
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.18"
                     /tool="antismash"
     gene            complement(1916..2125)
                     /locus_tag="EB41_RS16145"
     CDS             complement(1916..2125)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622435.1"
                     /locus_tag="EB41_RS16145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_033623988.1"
                     /transl_table=11
                     /translation="MLLLMNTTVTEIFMKTYDYQGSVIKEDNKIASIAYVQCVCGCLAS
                     RVSSNSDKYKCSWCKRTYTLRSVK"
     gene            complement(2073..2591)
                     /locus_tag="EB41_RS15535"
                     /old_locus_tag="EB41_02938"
     CDS             complement(2073..2591)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622434.1"
                     /locus_tag="EB41_RS15535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02938"
                     /product="hypothetical protein"
                     /protein_id="WP_010785310.1"
                     /transl_table=11
                     /translation="MVRITKKEFKPYNDYVDRPFELKWPTAFPLGELQAAIKETNDYHS
                     REIERLPQQSQGQIEHLLSRSIKQNKVLEIQLNSLDEYERVKPSIRGVFRGMAEFDVVL
                     IGDQQIDFYDIRHIQIHDFYKWSENEPEKPIDNPFSEVEFTGLTEEENSAIDAFIDEYY
                     SDGDFYEDV"
     misc_feature    2622..2635
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 24.56"
                     /tool="antismash"
     misc_feature    complement(2978..2987)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            complement(3053..3324)
                     /locus_tag="EB41_RS15545"
                     /old_locus_tag="EB41_02939"
                     /pseudo=""
     CDS             complement(3053..3324)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632800.1"
                     /locus_tag="EB41_RS15545"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02939"
                     /product="bacteriocin immunity protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDIKKKEALEKIIQLTNNALANPQIYSDKNLNNLLLRIRKEALSG
                     EVFYDLKKNYNQQLVDLR"
     gene            complement(3349..3633)
                     /locus_tag="EB41_RS15550"
                     /old_locus_tag="EB41_02940"
     CDS             complement(3349..3633)
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339190.1"
                     /locus_tag="EB41_RS15550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02940"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_010709169.1"
                     /transl_table=11
                     /translation="MDKQQELLDLLSKAYNDPKINEYEGLKDKLFECAKRLTTNETNIG
                     EVCYKLSTINSEYLARYHYEMPKSIIELQKFVTKEGQKYRGWASIGIWS"
     gene            complement(3747..4550)
                     /locus_tag="EB41_RS15555"
                     /old_locus_tag="EB41_02941"
     CDS             complement(3747..4550)
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /locus_tag="EB41_RS15555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02941"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_010709168.1"
                     /sec_met_domain="Abi (E-value: 9.9e-16, bitscore: 50.1,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQIFLQRYDNKEMKKSIFFKIIMVVVIYVVFLISNIGIEYIPIED
                     PSILKSVLQFGFNCILAWISYLLVRTFKLTNDRLLTSKITNIWLAILGIILVLFLLIVI
                     NIPKNTEDIFSGGLNLLVNSFFVAIAAAVFEEFLVRLLAFSSFLEIFKKNKYSLVFASI
                     ASSFLFGVLHISNLTTQSFEATMQQIFYATVLGLCFSVIRIRFNGLKYVIFLHFLIDFQ
                     PTIIDKGTAASSWEQIFLVFIPIAIVSIICLIQLNKDNKNLNYLF"
     gene            complement(4659..5000)
                     /locus_tag="EB41_RS15560"
                     /old_locus_tag="EB41_02942"
     CDS             complement(4659..5000)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367792.1"
                     /locus_tag="EB41_RS15560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02942"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_010709167.1"
                     /transl_table=11
                     /translation="MIDIEETKKIIHELYNSLMKRDKTKAILDITDVLLQVYKKIDSEK
                     YPEILINKMVNYIYIVGFDNKIHFLGNDEKLLIELGDISKKAGINSKYKANFTDKSQFY
                     SYSEKVPIR"
     gene            complement(5001..5216)
                     /locus_tag="EB41_RS15565"
                     /old_locus_tag="EB41_02943"
     CDS             complement(5001..5216)
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_II"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367791.1"
                     /locus_tag="EB41_RS15565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02943"
                     /product="leucocin A/sakacin P family class II bacteriocin"
                     /protein_id="WP_010709166.1"
                     /sec_met_domain="Bacteriocin_II (E-value: 1e-18, bitscore:
                     58.9, seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKLVKGLVICGMIGIGFTALGTNVEAATYYGNGVYCNKQKCWV
                     NWGQAWSEGVKRWGDNLFGSFSGGRI"
     misc_feature    5414..5430
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.98"
                     /tool="antismash"
     gene            5479..5631
                     /locus_tag="EB41_RS16335"
     CDS             5479..5631
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014725.4"
                     /locus_tag="EB41_RS16335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="preprotein translocase subunit YajC"
                     /protein_id="WP_341350519.1"
                     /transl_table=11
                     /translation="MLIMLLVIVAMYFYLFRTQKKQQKERQDFFKQFTTRRRCCDYWRV
                     TWSNF"
     gene            5561..5719
                     /locus_tag="EB41_RS16250"
     CDS             5561..5719
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014725.4"
                     /locus_tag="EB41_RS16250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="preprotein translocase subunit YajC"
                     /protein_id="WP_231433685.1"
                     /transl_table=11
                     /translation="MFLNNLQPGDAVVTIGGLHGVISEISSDKKKVTLDCEGAFFDFDQ
                     QSIRAKK"
     gene            complement(5742..5942)
                     /locus_tag="EB41_RS15575"
                     /old_locus_tag="EB41_02944"
     CDS             complement(5742..5942)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632412.1"
                     /locus_tag="EB41_RS15575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02944"
                     /product="hypothetical protein"
                     /protein_id="WP_010709164.1"
                     /transl_table=11
                     /translation="MAKKSKDLIDIGSFVHCQSVMGQTLKGKVEVINQYTVIVFDGEFR
                     HVVTKKQLKELGYSLPNKRKK"
     gene            6560..6877
                     /locus_tag="EB41_RS15580"
                     /old_locus_tag="EB41_02945"
     CDS             6560..6877
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616746.1"
                     /locus_tag="EB41_RS15580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02945"
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="WP_002394802.1"
                     /transl_table=11
                     /translation="MLITTSEKLYGVEYETLDVVFGVTTHSKNIVKNIGAGLKNLVGGE
                     IKAYTEMQQEARKIAMDRLKEEAKNIGADAIIAMRFDSGSISNDMQSVVAYGTAVKFKN
                     Q"
     gene            6913..7581
                     /locus_tag="EB41_RS15585"
                     /old_locus_tag="EB41_02946"
     CDS             6913..7581
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397511.1"
                     /locus_tag="EB41_RS15585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02946"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_010709163.1"
                     /sec_met_domain="Abi (E-value: 1.5e-21, bitscore: 68.7,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYVKRIFSIFILFIISQIGITAFGVIKQLSLDAGEKNLNISSTL
                     VTVILVVINILFLIYLAKRMNIIKTNHPFLTKKNILIILVATLIDRGIAIGGTLLLNMQ
                     GHTTTANDSTLIKLFSGVSPVLVVLLLGIAAPIMEEIVFRGGIIGYLVENNALLAILIS
                     SFLFGIIHGPTNFISFGMYFFMGIILSVSYYKTKDLRVSISIHFLNNLFPAIAIAYGLI
                     "
     gene            7698..7952
                     /locus_tag="EB41_RS15590"
                     /old_locus_tag="EB41_02947"
     CDS             7698..7952
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632522.1"
                     /locus_tag="EB41_RS15590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02947"
                     /product="hypothetical protein"
                     /protein_id="WP_010709162.1"
                     /transl_table=11
                     /translation="MKKYLSTFILFFGVVANLLVLTPQLYIHSQQTVVGLILGIIGFIL
                     AIFNYKKGYKTYQKIAFILGGIINIYPVLYFTFLFFALG"
     gene            complement(8058..8813)
                     /gene="istB"
                     /locus_tag="EB41_RS15595"
                     /old_locus_tag="EB41_02948"
     CDS             complement(8058..8813)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="istB"
                     /gene_functions="other (smcogs) SMCOG1151: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /locus_tag="EB41_RS15595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02948"
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="WP_010709161.1"
                     /transl_table=11
                     /translation="MSNYYQLLNKLEDLNLKHTREILPEYLDTIVQENISFVEALNHLM
                     TEEINARSERQKENRLRKANLPFKKTINDFDFGFQPKINRTEILDLCTLRFMDTNNNIL
                     FIGNSGVGKTHLAIAITLEAITKGYSCHFALSNELVNRLNRANQRGNLDRLLKKYASYD
                     LLIIDEIGYLPFTPDGAKLFFQLINLRYEKKSTIITTNIPLSQWAETFQDKKLTNALID
                     RLVHHAKIIQITGKSYRIKDYKEKKLSYK"
     gene            complement(8826..10052)
                     /gene="istA"
                     /locus_tag="EB41_RS15600"
                     /old_locus_tag="EB41_02949"
     CDS             complement(8826..10052)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="istA"
                     /gene_functions="other (smcogs) SMCOG1213: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331415.1"
                     /locus_tag="EB41_RS15600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EB41_02949"
                     /product="IS21 family transposase"
                     /protein_id="WP_010709160.1"
                     /transl_table=11
                     /translation="MRKDIYEGVLPFIMTHTKPNYAALAKQYNCDYRTVKRYYEAGISN
                     KLPLLAQKNKNKKTLVSDFEDIIADKLALGCSSSAIYHFISKRGYRGSARTLRRYCRNI
                     RADKIQKATIRVETTPGLSAQVDWKEDLKLVNKFGEVISCNIFLYVLGYSRIKYLELTF
                     DRSQTTLFRCLTNAFQYTGGVPTEIWFDNMKTVVDRSRSQFSKVVFNERFRQYAKEAGF
                     LPIACRAFRPQTKGKVEALARTIERIRVFNYEFESTHELERIIQTIMDSLNTEEISQAI
                     HECPSDRWSYEKEQLSSFDANQLKIYSSVEKQIKRKVSNEAMVQWNQNKYSVPVNYIGK
                     ILTLRVKEDKLYIYDDSTLIRTHTITHQLLNYHRNDYIEILRSDVFKHLEDEELERFVD
                     ENLQAYDEL"
ORIGIN
        1 taattcgaat actatcagca cttcttttac tgttaccttg ttgtttaatt aatttttcaa
       61 tatttttttt atttaggaca atgttttctt gtgtagtttt tagttcttta ttattagaag
      121 tagttgaggt agataaattt gaatcttctt ttcctgcacc aagaagacgt tcagcagctg
      181 atttccctct attattactc atttaaatct ccaactttct ctaaaaattc ttttgtaaac
      241 tggatataat cgtcttcagc agttgaagtc ttattaaagc tgattaagtc agaacctgct
      301 gcttgagctt catcaacagt gatagtacgt ctaatgtaag tatcaaaaat gtttacgtga
      361 agcgcgtcgg aaatttgatt tgctgtttta ttcatttctt ttgaaatatt aaatctttca
      421 ttatattttg tgaaaagaac tcctcgtatg attaagttct cattggtgta ttctttaaca
      481 gtctcgattg tttcattcaa tttaactaat ccttttagtg aaaaatatgc cgcttctaca
      541 ggaataataa tttcatcaga gacagtaaac gcatttactg ttaatatacc caagctagga
      601 gggcaatcaa taataatata atcatagttt tcttcaattg gttgaagaac tcttttaagt
      661 ctatgttcac taccaacttg agtaaattca cgctctgctg aagaaagagc aatatcagca
      721 ggtattacct cgattccttt gtaatttaaa atagattcag taatcgaagc ttcatctttt
      781 aatacttcat aaattgttgg aatattcgta ccatcaatat caaatccaaa attatcagta
      841 gcatttcctt gtgggtccat gtcaattaca agaactcttt tgttaaaagt aaaatgtaat
      901 gccgctccaa ggttaatgct agtcgttgtc tttgaaacac cgcctttttg gtttgcgact
      961 gaaattattc gtgccataat aatgtcctcc ttgtaatgtt atgctattat tatatcacga
     1021 tattgtgata tttcaatatt ataatagcat aaatttttaa tattacaata ttgtgtgggc
     1081 gtgtattcat gattttacaa tattgtagta tcgttatatt gtaatgacac aatattattt
     1141 taatgtacgg atgttccagt gacccctaaa atagtaaaaa ataatttatt gtaaaggagt
     1201 gttattcaat aattttttta gtacgtctgt gacgttctaa gtgtagcaaa taatcgtctt
     1261 gcagttttag aaggtctgaa tagacctgat ccatagtctt gctggctgat aaagaaagag
     1321 aataaataaa tgagatagga agagtttcaa tttttttgtt gcgagtaatc caagaagaaa
     1381 cagttccttg agagaaagcg tgtaattcac aaaattcttc aacagtaatc cctagttgct
     1441 tgataatgta tgtgttgata gggtgtggat acacaaaggt ttttctaggc atggtgggta
     1501 attttccttt ctcattttat taatgcgttt gcagtaatat tttatcaaac tctaagcaat
     1561 aagggaagga cattccagct tctttttggt ttgacctcct taaataatat gttatactat
     1621 aggtgcgaag cgaaattaat cgaacatgaa acaccaaaaa tccctcctag tgcaatagga
     1681 gggatttttt tgcgctgttg cgactcagcg ttgggattag aagcaactgc tttggttatc
     1741 tactaatcgt ctaaccatcg atcaacaagt aagagtacaa gacccactaa aagtggggcg
     1801 ataaccagcg aaattaattt ctcgaacatg aaaacacccc ctcacggggc agttgcctca
     1861 ataagtatag cataactttt aactccacct gcacagtagt taaatcttca gtaatttatt
     1921 taactgacct aagagtgtac gtccgcttac accaggaaca tttatactta tcagaattac
     1981 tcgacactct gcttgccaaa cagccacaca cacattggac ataagcaata ctagctattt
     2041 tgttatcttc tttgatcact gatccttgat aatcatacgt cttcataaaa atctccgtca
     2101 ctgtagtatt catcaataaa agcatcgatg gcgctgtttt cttcttctgt taatccagta
     2161 aactctactt ctgaaaaagg attgtctatc ggtttttcgg gttcgttttc cgaccattta
     2221 taaaaatcat gtatttgtat atgcctaatg tcgtaaaaat cgatttgctg atctccaatg
     2281 agaacgacat cgaattcagc catgccacga aatacaccac gtatagaagg ttttacacgc
     2341 tcatattcat ctaaggaatt aagctgaatt tctagaacct tattctgttt gattgaacga
     2401 cttagcaagt gttctatttg accctgtgac tgctggggta aacgttcaat ttctcttgaa
     2461 tgataatcat tcgtctcttt gattgctgct tgtaattccc ctaaaggaaa ggctgttggc
     2521 cacttcaatt caaagggtct atcgacgtaa tcattgtatg gtttgaattc ttttttcgtt
     2581 attcttacca ttttgaacac tctccttact tgaacattat acgaacaaac gttcttataa
     2641 tcaagtgcta gatttattgt atacctgaaa acttttagtt tggttggact gaacgaagag
     2701 aggggtacca aactaaaacg tcgaaggcca gttgagcaaa ttgaaactaa ttattcgctt
     2761 tttctgattt ggccatgttc ctggtataaa aaatcattcg tatttaaata gaaatttttt
     2821 tattatttcc acctgtagat caccatttaa tcatgtaaaa gcgaacgtta aaacatcttc
     2881 attgcataac taccaatcaa tatttgtgaa aatggcttag aattgctctc tcaaattaac
     2941 cgtatagtta aattcaacct atattttaag attctagtca aaaaacaact aaaaaactca
     3001 ctgctctaat caacagtgag tttttgtgta atacatgcta gcttataatt aattaaaatc
     3061 ctgaccatcc tttaggtgtt tgtatcaaag ttaataattc taacagttca ctaggcgttt
     3121 ggaaattatt tcttaacgta aatccactaa ttgttggttg taattctttt ttaaatcata
     3181 gaatacttct cctgatagag cttcttttct tattcttaat aaaagattat ttaagttttt
     3241 atctgaataa atctgtggat tcgctaatgc attatttgtt aattgaataa tcttttccaa
     3301 cgcttctttt ttcttgatgt ccataaactc tcctaaaaaa acgattgttt aactccaaat
     3361 accgatagac gcccagcctc tatatttttg gccctcttta gtcacaaatt tttgcaattc
     3421 aataattgat ttaggcattt cataatgata tcttgctaaa tactcactat taattgtact
     3481 taatttataa cagacttcac cgatatttgt ttcattagtt gtcaatcttt tggcacattc
     3541 aaataactta tcttttaacc cctcatattc atttatttta ggatcattat acgctttact
     3601 aagtaaatct agtaattctt gttgtttatc cattttaaac taatccttcc tatctaggag
     3661 cctagggctc atgctacacc caaccatttt ttcaatttcc cttgaagctt tcagcttttc
     3721 ctatctttta tacaactttg tcctttttaa aacaaataat tcaaattctt attatcttta
     3781 tttaattgaa ttaagcaaat aatagacaca atcgcaattg gtataaatac taaaaatatt
     3841 tgttcccaag acgatgccgc agttcctttg tcaattattg ttggctgaaa atcgattaaa
     3901 aaatgcaaga aaataacata ttttaatcca ttaaatcgaa ttcggattac agagaagcat
     3961 aagcctaaaa ctgttgcata aaaaatttgc tgcatggttg cttcaaaaga ttgcgttgtt
     4021 aaattagaaa tgtgcaatac tccaaataaa aaggaagacg ctattgaagc aaaaactaaa
     4081 gaatacttat tttttttaaa tatttctaaa aatgatgaaa atgctaataa tctaactaaa
     4141 aattcttcaa atacagctgc tgcaatagct acaaaaaaag aatttactaa taaatttagt
     4201 cctccactga agatatcttc agtatttttt ggaatattta ttactatcaa taaaaacagc
     4261 actagaatta ttcctaatat cgctagccag atatttgtaa ttttactagt taacaatcta
     4321 tcattagtta acttaaacgt tcttactaat aaatatgaaa tccacgctaa aatacagtta
     4381 aaaccaaatt gtaaaacgga ttttaaaata gacggatctt ctataggtat atattctata
     4441 cctatattac taattaaaaa aactacatat ataactacta ccataataat tttaaaaaat
     4501 atcgatttct tcatctcttt attatcatac ctttgtaaaa aaatttgcat acctataaat
     4561 tttcatccac aactaaattc caatatcaga gtaaataact acaaagttgt ctaggacaac
     4621 aagattttgt aattatttgg ttgaacgcag aaccattact acctaatagg tactttttca
     4681 gaataactat agaattgaga cttgtctgtg aaattagctt tatatttact attaattcca
     4741 gcctttttag atatatctcc caattcaatt aataactttt catcatttcc taaaaaatga
     4801 attttattgt caaatccaac aatatagata taattcacca ttttatttat taatatttct
     4861 ggatattttt cactatctat ttttttatag acttgcagta aaacatctgt aatatctaat
     4921 atagctttgg tcttatcacg cttcattaaa ctattatata gttcatgaat tatttttttt
     4981 gtttcctcaa tatcaatcat ttaaatcctt cctccactaa aactaccaaa caaattatct
     5041 ccccaacgtt taactccttc tgaccaagct tgtccccaat ttacccaaca tttttgctta
     5101 ttacaataca caccatttcc gtaatacgta gcagcttcta catttgttcc taatgctgta
     5161 aaaccaatcc caatcatgcc acaaataact aagcctttaa ctaatttctt tttcatgaat
     5221 gttcacctcg aaaaataaaa attagaaaac gtcttctata tagaaattat agaactattg
     5281 gtatagtttt gtaaatgtta aaacaaaaga atttctctaa catttttgta agttggtatt
     5341 tatcaatttc aattgttttt tacctgatta ttgtaccatt tcattcgaaa caaatattgc
     5401 catcaatact ttctttttat aaaataacct tgatgtctat atataaattt ttcaaattaa
     5461 caaggagaaa tgaaaccgat gcttatcatg ctgctagtta ttgttgctat gtatttttat
     5521 ctgtttcgta cacaaaaaaa gcaacaaaaa gaacgacaag atttttttaa acaatttaca
     5581 accaggagac gctgttgtga ctattggagg gttacatgga gtaatttctg aaatatcttc
     5641 agataaaaag aaggtaactc tcgactgtga aggagcgttt ttcgactttg atcaacaatc
     5701 tatacgagcg aaaaaataag cagaaaggaa tccctctctg cttatttttt tcttttattt
     5761 ggcaaagaat agcctaattc tttcaactgt tttttcgtga ccacatgacg aaattctcca
     5821 tcaaaaacaa tcactgtata ttgatttatc acttcaactt ttcctttgag cgtttgtccc
     5881 atgacagatt gacaatgcac aaaggatccg atatctatta aatctttact ttttttggcc
     5941 aaaacacaac ctccgaaaat aatgattaaa accacctagc aagtaggtgg tatggaagaa
     6001 atagtttctg aagaacaatt tcaacgtaac agtctataaa aattttatcg tatttacttt
     6061 cccatttcaa tcttaacttc tctaaaaaat cttgatacac tcattcatga cttttattta
     6121 actaagagga aatcatgaat atactctttt taagtattta ccttttttat gtaacctcta
     6181 tcttggtagg aatttatcaa tggctaaaaa agaaataaag aagttataaa aaatttcact
     6241 ctataagaat aaaggcaata accacttaaa atcgagggtt aaattcatga cactattact
     6301 attggcgaag tggttattac ctaaatttat tttatcaaaa attcaataaa tttaaatact
     6361 tttcatctcc ttagaatgcc tatattaatt ctcttctata aaaaaacaac agttatattt
     6421 attgataaga tcaatattta tgatatctaa tcaattttag tcagttactt taagttataa
     6481 aataacaata ttgtaagttt tttttctctc tttcatatgc tatatttata atataaaatt
     6541 ttgaaagggg ttttttaata tgctcataac aacttcagaa aaattatatg gtgtagaata
     6601 cgaaacactt gatgtagtat ttggtgtaac aacacattct aaaaatattg taaaaaatat
     6661 tggcgcagga ttaaaaaatt tagttggcgg agaaatcaaa gcgtatacag aaatgcaaca
     6721 agaagcacga aaaatagcaa tggaccgttt aaaagaagaa gcaaaaaata ttggggccga
     6781 cgctattatt gctatgcgtt tcgactctgg atctattagt aatgacatgc agtcagttgt
     6841 agcatatggt acagctgtta aatttaagaa tcaatagaaa aaacgaacat tggttttaat
     6901 aggagtgatt atatgaacta tgtaaaaaga atattttcaa ttttcatatt atttataatt
     6961 agtcaaattg gtatcactgc ttttggagta ataaaacaat tatctttaga tgcaggagaa
     7021 aaaaacttaa atatttcctc gacacttgtt acagtcatac ttgttgttat taatattctt
     7081 tttttaatct acttagctaa aagaatgaac attataaaaa caaatcatcc atttcttact
     7141 aaaaaaaata ttttgattat tttagtagca acattaatag atcgaggcat agctattggt
     7201 ggaacattgt tattaaatat gcaaggacac acaactacag caaatgattc gactcttatt
     7261 aaattattta gtggagtatc tcctgtttta gtggttttat tgttaggaat tgctgcacca
     7321 attatggaag aaattgtatt tagaggaggt atcataggat atcttgtaga aaataatgcc
     7381 ctattagcaa tattaatcag ttctttttta tttgggatta ttcatggccc tacaaatttt
     7441 atcagctttg gaatgtattt cttcatgggg attatccttt cagtttctta ctacaaaaca
     7501 aaagacttaa gagtaagcat atccatacac tttttaaaca acttattccc tgcaattgct
     7561 atagcttatg gtcttatata ataagctaac aaaagattaa caagttttga aaatatgtaa
     7621 tatttactaa tgctttttta tcatatttta taggtataaa tctttatact cttaactaat
     7681 cttaaggagg aaactatatg aaaaaatatc tatctacatt cattctcttt ttcggagtag
     7741 tagcaaacct tttagtactg acaccacaac tatatattca ttctcaacaa acggtagtag
     7801 gactaatttt aggaattatt ggttttattt tagctatttt caattataaa aaaggatata
     7861 aaacttacca aaaaattgct tttatcttag gtggtattat caacatttat ccagttttat
     7921 attttacatt tctatttttt gcacttggtt aattttactt ctgtcaatgg ccaagttaaa
     7981 atgtaggttt ttggccattt agaaatgtcg ttttttggcc accaaaaatg taggtttttg
     8041 gcccataaat aacaaggtta cttatacgac aacttttttt ctttataatc tttaattcga
     8101 taagactttc cagtaatttg aataatctta gcgtggtgca ctaatcgatc gattagtgca
     8161 tttgttaatt ttttatcttg aaaagtttca gcccattgag acaaaggaat attcgttgta
     8221 ataatggttg attttttttc atagcgtaga ttaattaatt gaaagaacaa ctttgccccg
     8281 tcaggggtaa aaggcaaata accaatttca tcaataatta gtaaatcgta agaagcatat
     8341 ttttttaaca atcgatctaa atttccacgt tgatttgcac gatttagtcg attgactaat
     8401 tcattgctta aagcaaaatg gcaagaataa cctttggtta tagcttctag tgtaatggca
     8461 atagctaagt gagttttccc cactccgcta tttccaataa acaatatatt attatttgta
     8521 tccataaagc gtaaggtaca caaatctaaa atctctgttc gattaatctt tggttgaaat
     8581 ccaaaatcaa agtcattgat tgttttttta aacggtaaat tcgcctttct taaacgattt
     8641 tctttttggc gttcacttcg tgcgtttatt tcttccgtca ttaaatgatt taacgcttct
     8701 acaaaactaa tattttcttg aacaatggta tcgagatatt ctggtaaaat ttctctcgta
     8761 tgttttaaat ttaaatcttc taatttgttt agtaattgat agtaattaga cataatgatt
     8821 cacctctata attcgtcata cgcttgtaaa ttttcatcaa caaagcgttc taattcttcg
     8881 tcttctaaat gtttaaacac atcagaacgt agaatctcta tatagtcgtt tcgatgataa
     8941 ttaagcaatt gatgagtaat tgtatgtgtg cgaataagag tggagtcatc gtagatataa
     9001 agtttatctt cttttacacg aagtgtaaga attttaccga tgtaatttac tggcacagaa
     9061 tatttatttt gattccattg aaccatcgct tcgttagata cttttcgttt aatttgtttt
     9121 tcaacagaag aatagatttt aagttgattc gcatcaaaag aagatagttg ttctttttca
     9181 tatgaccaac ggtcactagg acattcatga atcgcttgag aaatttcttc agtatttaat
     9241 gaatccatga ttgtttgaat aattctctct aattcatgag tggattcaaa ttcataatta
     9301 aatacacgaa ttcgttcgat tgttcgtgca agagcttcaa ctttaccttt tgtttgaggc
     9361 cgaaaagcac gacaagcaat tggaagaaat cccgcttctt tcgcatattg tcgaaatcgc
     9421 tcgttaaaaa caactttaga aaattgactt cgactacggt caacaacagt tttcatatta
     9481 tcaaaccata tttccgtagg aacacctccg gtgtattgaa aggcattagt taagcaacga
     9541 aatagagtag tttgagaacg atcaaaagtt agttctaagt acttaattct ggaatatcct
     9601 aaaacgtaaa gaaagatatt acaagaaata acttcgccga atttattgac taattttaga
     9661 tcttctttcc aatcaacttg agcagataaa ccaggggttg tttccacacg aatcgttgct
     9721 ttttgtattt tatctgctcg gatatttcga caatatcttc ttagtgttcg tgcacttcca
     9781 cgatagcctc gtttagaaat aaaatgataa atggcagaag aagaacaacc taaagctaat
     9841 ttgtcagcga taatatcttc aaaatcacta accaaagttt ttttgttttt attcttttga
     9901 gcaagtaaag gcaatttatt gcttattcca gcttcataat aacgttttac tgtacgataa
     9961 tcgcaattgt attgtttagc tagagcagca taattaggtt ttgtatgagt cataatgaaa
    10021 ggtaacactc cttcataaat atcttttctc aatacgcttt cctccttgaa taaaataacg
    10081 caaggaaagc gtaccagatg acgcataaac ctacattttt atttggccat ttttctacat
    10141 tttataatat aatgtagtat tctttagaat ctaacaactt aaaaaatccc acaagaattc
    10201 atttgataac tttttt
//
