GCF_003315125.1 - 16 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_QNRN01000001.1 (Bacillus sp. DB-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	terpene-precursor	1	20,308

NZ_QNRN01000002.1 (Bacillus sp. DB-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 2.1	terpene	434,660	456,513

NZ_QNRN01000004.1 (Bacillus sp. DB-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 4.1	RiPP-like	97,715	107,987
Region 4.2	betalactone	197,289	222,527	Low	fengycin	NRPS:Type I
Region 4.3	RiPP-like	324,068	334,379

NZ_QNRN01000006.1 (Bacillus sp. DB-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 6.1	lassopeptide	258,987	282,966	High	paeninodin	ribosomal:unmodified

NZ_QNRN01000009.1 (Bacillus sp. DB-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 9.1	NRPS	1	62,544

NZ_QNRN01000010.1 (Bacillus sp. DB-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 10.1	NRPS,lanthipeptide-class-ii	1	31,935	High	thuricin	ribosomal:RiPP
Region 10.2	lanthipeptide-class-i	84,620	109,019
Region 10.3	RiPP-like	208,201	219,439

NZ_QNRN01000012.1 (Bacillus sp. DB-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 12.1	azole-containing-RiPP	146,048	179,772

NZ_QNRN01000013.1 (Bacillus sp. DB-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 13.1	NRP-metallophore,NRPS	143,826	195,586	High	bacillibactin	NRPS:Type I

NZ_QNRN01000015.1 (Bacillus sp. DB-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 15.1	NRPS-like	77,694	121,275

NZ_QNRN01000018.1 (Bacillus sp. DB-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 18.1	NI-siderophore	47,408	79,125	High	petrobactin	other:other

NZ_QNRN01000030.1 (Bacillus sp. DB-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 30.1	azole-containing-RiPP	1	21,375

NZ_QNRN01000031.1 (Bacillus sp. DB-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 31.1	cyclic-lactone-autoinducer	1	14,383

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	terpene-precursor	1	20,308
Region 2.1	terpene	434,660	456,513
Region 4.1	RiPP-like	97,715	107,987
Region 4.2	betalactone	197,289	222,527	Low	fengycin	NRPS:Type I
Region 4.3	RiPP-like	324,068	334,379
Region 6.1	lassopeptide	258,987	282,966	High	paeninodin	ribosomal:unmodified
Region 9.1	NRPS	1	62,544
Region 10.1	NRPS,lanthipeptide-class-ii	1	31,935	High	thuricin	ribosomal:RiPP
Region 10.2	lanthipeptide-class-i	84,620	109,019
Region 10.3	RiPP-like	208,201	219,439
Region 12.1	azole-containing-RiPP	146,048	179,772
Region 13.1	NRP-metallophore,NRPS	143,826	195,586	High	bacillibactin	NRPS:Type I
Region 15.1	NRPS-like	77,694	121,275
Region 18.1	NI-siderophore	47,408	79,125	High	petrobactin	other:other
Region 30.1	azole-containing-RiPP	1	21,375
Region 31.1	cyclic-lactone-autoinducer	1	14,383

No secondary metabolite regions were found in these records:: NZ_QNRN01000003.1 (Bacillus sp. DB-2); NZ_QNRN01000005.1 (Bacillus sp. DB-2); NZ_QNRN01000007.1 (Bacillus sp. DB-2); NZ_QNRN01000008.1 (Bacillus sp. DB-2); NZ_QNRN01000011.1 (Bacillus sp. DB-2); NZ_QNRN01000014.1 (Bacillus sp. DB-2); NZ_QNRN01000016.1 (Bacillus sp. DB-2); NZ_QNRN01000017.1 (Bacillus sp. DB-2); NZ_QNRN01000019.1 (Bacillus sp. DB-2); NZ_QNRN01000020.1 (Bacillus sp. DB-2); NZ_QNRN01000021.1 (Bacillus sp. DB-2); NZ_QNRN01000022.1 (Bacillus sp. DB-2); NZ_QNRN01000023.1 (Bacillus sp. DB-2); NZ_QNRN01000024.1 (Bacillus sp. DB-2); NZ_QNRN01000025.1 (Bacillus sp. DB-2); NZ_QNRN01000026.1 (Bacillus sp. DB-2); NZ_QNRN01000027.1 (Bacillus sp. DB-2); NZ_QNRN01000028.1 (Bacillus sp. DB-2); NZ_QNRN01000029.1 (Bacillus sp. DB-2); NZ_QNRN01000032.1 (Bacillus sp. DB-2); NZ_QNRN01000033.1 (Bacillus sp. DB-2); NZ_QNRN01000034.1 (Bacillus sp. DB-2); NZ_QNRN01000035.1 (Bacillus sp. DB-2); NZ_QNRN01000036.1 (Bacillus sp. DB-2); NZ_QNRN01000037.1 (Bacillus sp. DB-2); NZ_QNRN01000038.1 (Bacillus sp. DB-2); NZ_QNRN01000039.1 (Bacillus sp. DB-2); NZ_QNRN01000040.1 (Bacillus sp. DB-2); NZ_QNRN01000041.1 (Bacillus sp. DB-2); NZ_QNRN01000042.1 (Bacillus sp. DB-2); NZ_QNRN01000043.1 (Bacillus sp. DB-2); NZ_QNRN01000044.1 (Bacillus sp. DB-2)

Compact view

NZ_QNRN01000001.1 - Region 1 - terpene-precursor

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Location: 1 - 20,308 nt. (total: 20,308 nt) Show pHMM detection rules used

Region on contig edge.

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: carboxylase

DET63_RS00085: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

Ligases: synthase synthetase

DET63_RS00050: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

DET63_RS00055: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0002219.2	0.39	terpene	(-)-(R)-nephthenol	Stereum hirsutum FP-91666 SS1
BGC0000665.5	0.38	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0002284.3	0.38	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000639.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000638.3	0.37	terpene	carotenoid	Pantoea ananatis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0002219.2	0.39	terpene	(-)-(R)-nephthenol	Stereum hirsutum FP-91666 SS1
BGC0000665.5	0.38	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0002284.3	0.38	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000639.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000638.3	0.37	terpene	carotenoid	Pantoea ananatis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

DET63_RS00055: PT_FPPS_like

Domain 1 (33 - 296): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: IPP, DMAPP → FPP

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.49 out of 27.78

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.53 out of 25.88

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.28 out of 26.33

Confidence: medium

Additional weak hits (1):

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 25.40 out of 33.14

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
PsrA	Oleic acid responsive regulator

NZ_QNRN01000002.1 - Region 1 - terpene

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Location: 434,660 - 456,513 nt. (total: 21,854 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

DET63_RS05185: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

DET63_RS05190: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Lyases: lyase

DET63_RS05160: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000888.5	0.49	other (other)	bacilysin	Bacillus sp. CS93
BGC0001928.2	0.46	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000231.5	0.42	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001197.5	0.40	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0002493.3	0.40	NRPS	parabactin	Paracoccus denitrificans PD1222
BGC0001605.3	0.40	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0002218.2	0.39	terpene	(-)-Cyatha-3,12-diene	Dentipellis sp. KUC8613
BGC0002161.2	0.39	PKS	scytalone, T3HN	Pestalotiopsis fici W106-1
BGC0002138.2	0.38	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670
BGC0000234.5	0.37	PKS	jadomycin	Streptomyces venezuelae ATCC 10712

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000888.5	0.49	other (other)	bacilysin	Bacillus sp. CS93
BGC0001928.2	0.46	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000231.5	0.42	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001197.5	0.40	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0002493.3	0.40	NRPS	parabactin	Paracoccus denitrificans PD1222
BGC0001605.3	0.40	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0002218.2	0.39	terpene	(-)-Cyatha-3,12-diene	Dentipellis sp. KUC8613
BGC0002161.2	0.39	PKS	scytalone, T3HN	Pestalotiopsis fici W106-1
BGC0002138.2	0.38	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670
BGC0000234.5	0.37	PKS	jadomycin	Streptomyces venezuelae ATCC 10712

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	hopanoid		C30	hopanoid skeleton	unknown
triterpene	polypodane		C30	polypodane skeleton	unknown

Genes with terpene domains:

DET63_RS05200: T2TS

Domain 1 (4 - 614): Type 2 terpene synthase

Subtype: Squalene substrate

Reactions:: squalene → hopanoid; squalene → polypodane

Transcription Factor Binding Sites

Binding sites found:

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 21.97 out of 25.31

Confidence: strong

Additional weak hits (2):

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.31 out of 23.43

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 26.88 out of 31.73

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor

NZ_QNRN01000004.1 - Region 1 - RiPP-like

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Location: 97,715 - 107,987 nt. (total: 10,273 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

DET63_RS08325: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Similar gene clusters

Analysis type:

Reference	RiPP-like	Similarity score	Type	Compound(s)	Organism
BGC0001994.4		0.35	other (other)	β-ethynylserine	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0002373.3		0.19	NRPS	bonnevillamide D, bonnevillamide E	Streptomyces sp.

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0001994.4		0.35	other (other)	β-ethynylserine	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0002373.3		0.19	NRPS	bonnevillamide D, bonnevillamide E	Streptomyces sp.

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (2):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 26.71 out of 30.97

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.88 out of 30.98

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_QNRN01000004.1 - Region 2 - betalactone

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Location: 197,289 - 222,527 nt. (total: 25,239 nt) Show pHMM detection rules used

betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

DET63_RS08870: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: acetyltransferase carboxylase transferase

DET63_RS08815: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

DET63_RS08845: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

DET63_RS08865: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

Lyases: hydratase

DET63_RS08850: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

DET63_RS08840: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

DET63_RS08855: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002284.3	0.55	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000131.5	0.46	PKS	pyrrolomycin	Streptomyces sp. UC 11065
BGC0000706.3	0.46	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0000913.3	0.44	other (other)	MK-8	Enterobacter cloacae
BGC0000234.5	0.43	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002000.4	0.43	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0002034.3	0.43	other (other)	perquinoline A, perquinoline B, perquinoline C	Streptomyces sp. IB2014 016-6
BGC0001103.5	0.42	NRPS, PKS	mycosubtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0001226.4	0.42	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000623.3	0.42	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002284.3	0.55	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000131.5	0.46	PKS	pyrrolomycin	Streptomyces sp. UC 11065
BGC0000706.3	0.46	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0000913.3	0.44	other (other)	MK-8	Enterobacter cloacae
BGC0000234.5	0.43	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002000.4	0.43	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0002034.3	0.43	other (other)	perquinoline A, perquinoline B, perquinoline C	Streptomyces sp. IB2014 016-6
BGC0001103.5	0.42	NRPS, PKS	mycosubtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0001226.4	0.42	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000623.3	0.42	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 26.74 out of 28.14

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 15.94 out of 18.46

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.54 out of 18.46

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.90 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.23 out of 26.06

Confidence: medium

Additional weak hits (3):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.89 out of 32.74

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.04 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.02 out of 24.78

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MogR	Repressor of flagellar motility genes
PerR	Peroxide regulator
RpoN	Regulator of nitrogen assimilation and virulence

NZ_QNRN01000004.1 - Region 3 - RiPP-like

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Location: 324,068 - 334,379 nt. (total: 10,312 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase

DET63_RS09370: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000852.5	0.48	other (other)	ectoine	Sporosarcina pasteurii
BGC0000859.3	0.48	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000856.3	0.47	other (other)	ectoine	Methylophaga thalassica
BGC0000855.3	0.47	other (other)	ectoine	Methylomicrobium kenyense
BGC0000858.3	0.47	other (other)	ectoine	Methylobacter marinus
BGC0000857.3	0.47	other (other)	ectoine	Methylophaga alcalica
BGC0000860.3	0.46	other (other)	ectoine	Methylarcula marina
BGC0000853.3	0.42	other (other)	ectoine	Streptomyces anulatus
BGC0002052.3	0.41	other (ectoine)	ectoine	Streptomyces sp.
BGC0000854.3	0.41	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000852.5	0.48	other (other)	ectoine	Sporosarcina pasteurii
BGC0000859.3	0.48	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000856.3	0.47	other (other)	ectoine	Methylophaga thalassica
BGC0000855.3	0.47	other (other)	ectoine	Methylomicrobium kenyense
BGC0000858.3	0.47	other (other)	ectoine	Methylobacter marinus
BGC0000857.3	0.47	other (other)	ectoine	Methylophaga alcalica
BGC0000860.3	0.46	other (other)	ectoine	Methylarcula marina
BGC0000853.3	0.42	other (other)	ectoine	Streptomyces anulatus
BGC0002052.3	0.41	other (ectoine)	ectoine	Streptomyces sp.
BGC0000854.3	0.41	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.86 out of 26.06

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.36 out of 20.97

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.80 out of 29.72

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.52 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.52 out of 26.06

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 16.53 out of 18.16

Confidence: strong

Additional weak hits (1):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 20.13 out of 30.22

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CcpA	Catabolite control protein A
MogR	Repressor of flagellar motility genes
NtrC	Nitrogen regulatory protein C

NZ_QNRN01000006.1 - Region 1 - lassopeptide

Download region GenBank file

Download region SVG

Location: 258,987 - 282,966 nt. (total: 23,980 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase glycosyltransferase

DET63_RS12755: sugar transferase

smcogs: SMCOG1146: sugar transferase

DET63_RS12765: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Hydrolases: esterase hydrolase

DET63_RS12770: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

DET63_RS12835: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

smcogs: SMCOG1283: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

Ligases: synthase

DET63_RS12730: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001356.4	0.68	ribosomal	paeninodin	Paenibacillus dendritiformis C454
BGC0001634.3	0.52	ribosomal	keywimysin	Streptomyces sp. NRRL F-5702
BGC0000571.3	0.50	ribosomal	burhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002825.2	0.49	ribosomal	nocapeptin A	Nocardia terpenica IFM 0406
BGC0001410.3	0.47	saccharide	polysaccharide A	Bacteroides fragilis
BGC0000800.5	0.46	saccharide	xanthan	Xanthomonas campestris
BGC0000741.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000744.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000745.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000868.5	0.45	other (other)	teichuronic acid	Bacillus subtilis subsp. subtilis str. 168

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001356.4	0.68	ribosomal	paeninodin	Paenibacillus dendritiformis C454
BGC0001634.3	0.52	ribosomal	keywimysin	Streptomyces sp. NRRL F-5702
BGC0000571.3	0.50	ribosomal	burhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002825.2	0.49	ribosomal	nocapeptin A	Nocardia terpenica IFM 0406
BGC0001410.3	0.47	saccharide	polysaccharide A	Bacteroides fragilis
BGC0000800.5	0.46	saccharide	xanthan	Xanthomonas campestris
BGC0000741.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000744.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000745.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000868.5	0.45	other (other)	teichuronic acid	Bacillus subtilis subsp. subtilis str. 168

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.41 out of 27.78

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.25 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.30 out of 26.06

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 22.42 out of 24.78

Confidence: strong

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 28.44 out of 33.14

Confidence: medium

Additional weak hits (4):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 27.28 out of 39.62

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 25.83 out of 39.62

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 24.35 out of 33.73

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 19.47 out of 27.18

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

No core peptides found.

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 16 - 96; Score: 83.0

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
LexA variant 2	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes
ZuR variant 2	Zinc-responsive repressor

NZ_QNRN01000009.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 62,544 nt. (total: 62,544 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: PPTase methyltransferase

DET63_RS16295: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

DET63_RS16340: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: esterase hydrolase

DET63_RS16310: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

DET63_RS16345: thioesterase

smcogs: SMCOG1004: thioesterase

DET63_RS16430: isochorismatase

smcogs: SMCOG1027: isochorismatase

Ligases: ligase

DET63_RS16325: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

DET63_RS16330: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

DET63_RS16335: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001133.4	0.73	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001758.4	0.73	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.73	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001133.4	0.73	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001758.4	0.73	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.73	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.97 out of 27.78

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.05 out of 20.97

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.55 out of 19.26

Confidence: medium

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 24.39 out of 32.93

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.52 out of 26.06

Confidence: medium

Additional weak hits (6):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.06 out of 32.74

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 25.75

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 24.62 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 24.75 out of 36.54

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.00 out of 30.98

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 62544:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Thr) + (X - Ala) + (Ser - Leu - D-Thr - Glu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

DET63_RS16325: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (457..866): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LSTHFDFSVWEGNQIFGGEINMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

DET63_RS16330: X - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (466..870): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YWMTFDVSVWEWQFFSGGETNFYGPTEATIYVNA
Nearest Stachelhaus code(s):: Glu DVWHFGRINK (71% 8Å match); Gln DAWQFGLIDK (65% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1527..1919): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: MWATFDLSVFEVNTNLAGEYNLYGPSEDTTYSTY
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

DET63_RS16335: Ser - Leu - Thr - Glu

Search NORINE for peptide: strict or relaxed

AMP-binding (496..901): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFTSGEINLYGPTEATVDVTY
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1559..1946): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: MWHAFDSAVQEPFAITGGDINQYGPSENTVVTTD
Nearest Stachelhaus code(s):: Pro DVQFIAQVVK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2602..3008): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LSTHFDFSVWEGNQIFGGEINMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4124..4524): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: LGLAFDSSVKQMDILVGGEYNVYGPTECTVDATC
Nearest Stachelhaus code(s):: Glu DAKDLGVVDK (71% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
LexA variant 3	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes

NZ_QNRN01000010.1 - Region 1 - NRPS,lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 1 - 31,935 nt. (total: 31,935 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase decarboxylase

DET63_RS17565: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

DET63_RS17580: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Ligases: ligase

DET63_RS17530: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000626.3	1.50	ribosomal	thuricin	Bacillus thuringiensis
BGC0001787.3	0.44	ribosomal	suicin 3908	Streptococcus suis
BGC0001210.5	0.39	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000498.5	0.37	ribosomal	bovicin HJ50	Streptococcus equinus
BGC0000539.5	0.37	ribosomal	nukacin ISK-1	Staphylococcus warneri
BGC0000561.5	0.37	ribosomal	thermophilin 1277	Streptococcus thermophilus
BGC0000554.4	0.37	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001579.4	0.36	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000521.3	0.35	ribosomal	lacticin 481	Lactococcus lactis subsp. lactis
BGC0002135.2	0.34	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000626.3	0.91	ribosomal	thuricin	Bacillus thuringiensis
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.64	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.64	NRPS	AbT1	Aureobasidium pullulans
BGC0002286.3	0.63	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for DET63_RS17565

DET63_RS17570

Leader:

METEKYLQVVEDEEIEQLVGG

Core:

AGPGWVETLTKDCPWNVPVACVTIMGQRICKKCY

Core with dehydrations:

AGPGWVEDhbLDhbKDCysPWNVPVACysVDhbIMGQRICysKKCysY

antiSMASH-DB 4.0 matches for DET63_RS17570

100.0%

NZ_JH792312.1and 1 other:

NZ_QNRN01000010.1 Lanthipeptides: DET63_RS17570_lanthipeptide

Lanthipeptides: IIG_RS24665_lanthipeptide

1

AGPGWVETLTKDCPWNVPVACVTIMGQRICKKCY

34

AGPGWVETLTKDCPWNVPVACVTIMGQRICKKCY

1

AGPGWVETLTKDCPWNVPVACVTIMGQRICKKCY

34

97.1%

NZ_CP036100.1and 1 other:

NZ_CP072062.1 Lanthipeptides: J5V93_RS29280_lanthipeptide

Lanthipeptides: EXW47_RS29185_lanthipeptide

1

AGPGWVETLTKDCPWNVPVACVTIMGQRICKKCY

34

AGPGWVET+TKDCPWNVPVACVTIMGQRICKKCY

1

AGPGWVETVTKDCPWNVPVACVTIMGQRICKKCY

34

91.2%

NZ_CM000748.1and 1 other:

NZ_JAIPUK010000011.1 Lanthipeptides: K9P71_RS26815_lanthipeptide

Lanthipeptides: BTHUR0003_RS30910_lanthipeptide

2

GPGWVETLTKDCPWNVPVACVTIMGQRICKKC

33

GPGWVETLTKDCPWN+PVACVTIMGQRICKKC

2

GPGWVETLTKDCPWNMPVACVTIMGQRICKKC

33

with 42 more sequence matches

Only the first 10 are displayed here.

88.2%

NZ_KB976276.1:

Lanthipeptides: IIC_RS09105_lanthipeptide

1

AGPGWVETLTKDCPWNVPVACVTIMGQRICKKCY

34

AGPGWVETLTKDCPW PVACV IMGQ+ICKKCY

1

AGPGWVETLTKDCPWKQPVACVIIMGQKICKKCY

34

85.3%

NZ_CM000731.1and 2 others:

NZ_CP043834.1 Lanthipeptides: F0362_RS30375_lanthipeptideNZ_NVOB01000050.1 Lanthipeptides: CON62_RS29235_lanthipeptide

Lanthipeptides: BCERE0020_RS28875_lanthipeptide

1

AGPGWVETLTKDCPWNVPVACVTIMGQRICKKCY

34

AGPGWVETLTKDCPW P ACV IMGQ+ICKKCY

1

AGPGWVETLTKDCPWKQPAACVIIMGQKICKKCY

34

85.3%

NZ_CP036053.1and 7 others:

NZ_JAOPRA010000024.1 Lanthipeptides: OCE16_RS27090_lanthipeptideNZ_KB976667.1 Lanthipeptides: IG5_RS06165_lanthipeptideNZ_LDKD02000003.1 Lanthipeptides: ABH17_RS27060_lanthipeptideNZ_NUAO01000019.1 Lanthipeptides: CN544_RS19345_lanthipeptideNZ_NVFH01000027.1 Lanthipeptides: COL40_RS24675_lanthipeptideNZ_NVHY01000021.1 Lanthipeptides: COM05_RS26660_lanthipeptideNZ_NVOV01000033.1 Lanthipeptides: CON78_RS26470_lanthipeptide

Lanthipeptides: EXW64_RS27135_lanthipeptide

1

AGPGWVETLTKDCPWNVPVACVTIMGQRICKKCY

34

AGPGWVETLTKDCPW P ACV IMGQ+ICKKCY

1

AGPGWVETLTKDCPWKQPGACVIIMGQKICKKCY

34

73.5%

NZ_KB976165.1:

Lanthipeptides: ICC_RS24850_lanthipeptide

2

GPGWVETLTKDCPWNVPVACVTIMGQRICKKC

33

GPGWVETLTKDCP P AC+TI GQ ICKKC

2

GPGWVETLTKDCPGYKPYACITIAGQTICKKC

33

70.6%

NZ_CM000758.1and 1 other:

NZ_MOOT01000057.1 Lanthipeptides: BK765_RS20265_lanthipeptide

Lanthipeptides: BTHUR0013_RS15850_lanthipeptide

2

GPGWVETLTKDCPWNVPVACVTIMGQRICKKC

33

GPGW+ TLTKDCP N P AC+TI GQ ICKKC

2

GPGWIATLTKDCPANKPYACITIAGQTICKKC

33

58.8%

NZ_FNYB01000007.1and 1 other:

NZ_KB976303.1 Lanthipeptides: IGY_RS28100_lanthipeptide

Lanthipeptides: BMZ34_RS15340_lanthipeptide

2

GPGWVETLTKDCPWNVPVACVTIMGQRICKKCY

34

GPG++ETLTKDCP P CV I G CK+CY

2

GPGYIETLTKDCPRVTPQICVPINGIWTCKRCY

34

50.0%

NC_017621.1and 16 others:

NZ_CP039462.1 Lanthipeptides: E8M06_RS05390_lanthipeptideNZ_JABTZM010000032.1 Lanthipeptides: HRQ47_RS09675_lanthipeptideNZ_JAEVFQ010000010.1 Lanthipeptides: JNO64_RS06375_lanthipeptideNZ_JAFFHD010000003.1 Lanthipeptides: JN532_RS02075_lanthipeptideNZ_JAGFVG010000007.1 Lanthipeptides: J5581_RS04225_lanthipeptideNZ_JAIJZO010000001.1 Lanthipeptides: K5Q34_RS00965_lanthipeptideNZ_JAIMDS010000024.1 Lanthipeptides: K6V32_RS10650_lanthipeptideNZ_JAJUWV010000001.1 Lanthipeptides: LZF89_RS05505_lanthipeptideNZ_JAMDIS010000017.1 Lanthipeptides: M5P45_RS08180_lanthipeptideNZ_JAMYIU010000001.1 Lanthipeptides: NKE44_RS01780_lanthipeptideNZ_JAMYIX010000001.1 Lanthipeptides: NKE54_RS02675_lanthipeptideNZ_JAMYIY010000001.1 Lanthipeptides: NKE64_RS02785_lanthipeptideNZ_JAMYJP010000014.1 Lanthipeptides: NKE68_RS07350_lanthipeptideNZ_LR738722.1 Lanthipeptides: STREPSUIS_RS03955_lanthipeptideNZ_VZMP01000023.1 Lanthipeptides: F6P50_RS09295_lanthipeptideNZ_VZNS01000007.1 Lanthipeptides: F6P79_RS05700_lanthipeptide

Lanthipeptides: SSUD12_RS06540_lanthipeptide

1

AGPGWVETLTKDCPWNVPVACVTIMGQRICKKC

33

AG G+V+TLTKDCP + CV I CK C

1

AGSGFVKTLTKDCPGFLSNVCVNIGFISGCKNC

33

50.0%

NZ_AQQB01000068.1and 6 others:

NZ_BCEY01000024.1 Lanthipeptides: GOZ53_RS05100_lanthipeptideNZ_BCEZ01000067.1 Lanthipeptides: GOZ55_RS11230_lanthipeptideNZ_BCFC01000053.1 Lanthipeptides: GOZ35_RS07920_lanthipeptideNZ_BCFG01000017.1 Lanthipeptides: GOZ50_RS03220_lanthipeptideNZ_BCFI01000009.1 Lanthipeptides: GOZ54_RS02495_lanthipeptideNZ_CP019557.1 Lanthipeptides: BZG42_RS05725_lanthipeptide

Lanthipeptides: K738_RS01000000110290_lanthipeptide

1

AGPGWVETLTKDCPWNVPVACVTIMGQRICKKC

33

AG G V+TLTKDCP + CV I CK C

1

AGSGLVKTLTKDCPGFLSNICVNIGFISGCKNC

33

50.0%

NZ_CP040409.1and 4 others:

NZ_CP040414.1 Lanthipeptides: FE331_RS04020_lanthipeptideNZ_CP040415.1 Lanthipeptides: allorf_0721024_0721203_lanthipeptideNZ_CP040417.1 Lanthipeptides: FE328_RS00745_lanthipeptideNZ_CP040424.1 Lanthipeptides: FE321_RS03445_lanthipeptide

Lanthipeptides: FE336_RS03450_lanthipeptide

2

GPGWVETLTKDCPWNVPVACVTIMGQRICKKC

33

GPG ++TLTKDCP V CV + CKKC

2

GPGLLKTLTKDCPGTVSDVCVNLAVVSTCKKC

33

41.2%

NZ_CP040409.1and 4 others:

NZ_CP040414.1 Lanthipeptides: FE331_RS04030_lanthipeptideNZ_CP040415.1 Lanthipeptides: FE330_RS03440_lanthipeptideNZ_CP040417.1 Lanthipeptides: FE328_RS00755_lanthipeptideNZ_CP040424.1 Lanthipeptides: FE321_RS03455_lanthipeptide

Lanthipeptides: FE336_RS03460_lanthipeptide

1

AGPGWVETLTKDCPWNVPVAC

21

GPG V TLTKDCP VP C

1

VGPGLVRTLTKDCPNVVPKVC

21

MIBiG 4.0 matches for DET63_RS17570

20.6%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

4

GWVETLTKDC

13

GW TLTK+C

17

GWC-TLTKEC

25

DET63_RS17575

Leader:

KEPEKDILPYIEKHNLGAVIRG

Core:

PLKMGILTGKFNHETQFPGDDLRKDCPKENWFKDKLK

Core with dehydrations:

PLKMGILDhbGKFNHEDhbQFPGDDLRKDCysPKENWFKDKLK

antiSMASH-DB 4.0 matches for DET63_RS17575

100.0%

NZ_QNRN01000010.1:

Lanthipeptides: DET63_RS17575_lanthipeptide

1

PLKMGILTGKFNHETQFPGDDLRKDCPKENWFKDKLK

37

PLKMGILTGKFNHETQFPGDDLRKDCPKENWFKDKLK

1

PLKMGILTGKFNHETQFPGDDLRKDCPKENWFKDKLK

37

24.3%

NZ_JADEWH010000004.1:

Lanthipeptides: allorf_393272_393412_lanthipeptide

13

HETQFPGD-DLRKDCPKE

29

H +FP D DLR +C K

11

HFRKFPIDQDLRNNCQKS

28

21.6%

NZ_CP073819.1:

Lanthipeptides: KFV12_RS00360_lanthipeptide

18

PGDDLRKDCPKENWFKDKL

36

PG K+CPK NW +

9

PGT-CNKECPKTNWLCSNV

26

with 6 more sequence matches

20.0%

NZ_CP077726.1:

Lanthipeptides: KTC92_RS18445_lanthipeptide

5

GILTGKFNHETQFPGDDLRKDCPKE

29

GI+ +HE QF D +++ KE

16

GIIFVDCSHENQFE-DRVKRSSSKE

39

18.9%

NZ_LT745762.1:

Lanthipeptides: allorf_071320_071490_lanthipeptide

4

MGILTGKFNHETQF

17

+GIL GK + QF

2

IGILVGKSVAKAQF

15

16.2%

NZ_NGMO01000003.1and 3 others:

NZ_NGMO01000003.1 Lanthipeptides: allorf_391705_391890_lanthipeptideNZ_NGMO01000003.1 Lanthipeptides: allorf_392307_392492_lanthipeptideNZ_NGMO01000003.1 Lanthipeptides: allorf_391705_391890_lanthipeptide

Lanthipeptides: allorf_392307_392492_lanthipeptide

18

PGDDLRKDCPKENWFKDKL

36

PG +CP+ NW +

5

PGT-CNSNCPQTNWLCSNV

22

15.9%

NZ_CP021646.1and 1 other:

NZ_CP031759.1 Lanthipeptides: D0N37_RS18595_lanthipeptide

Lanthipeptides: B1L02_RS18305_lanthipeptide

12

NHETQFPGDDLRKDCPKENWFKDKL

36

N Q G D++ + P + F D+

1

NMTPQIAGGDVQANSPAGSIFTDRY

25

15.9%

NZ_CP024625.1and 3 others:

NZ_CP047238.1 Lanthipeptides: GSF04_RS22995_lanthipeptideNZ_CP068023.1 Lanthipeptides: JJQ94_RS23815_lanthipeptideNZ_CP081860.1 Lanthipeptides: K5642_RS18070_lanthipeptide

Lanthipeptides: CTT31_RS17865_lanthipeptide

12

NHETQFPGDDLRKDCPKENWFKDK

35

N Q G D++ + P + F D+

1

NMTPQIAGGDVQANSPAGSLFTDR

24

15.6%

NZ_CP077726.1:

Lanthipeptides: KTC92_RS18445_lanthipeptide

25

DCPKENWFKDKLK

37

DC EN F+D++K

21

DCSHENQFEDRVK

33

DET63_RS17600

Leader:

MNYERVAKNLINLRNEKSREEVARA

Core:

VGISVSTLQMYENGQRIPRDNIKIKLANFYGVTVQIIFFDSEQHEVC

Core with dehydrations:

VGIDhaVDhaDhbLQMYENGQRIPRDNIKIKLANFYGVDhbVQIIFFDDhaEQHEVCys

antiSMASH-DB 4.0 matches for DET63_RS17600

100.0%

NZ_QNRN01000010.1:

Lanthipeptides: DET63_RS17600_lanthipeptide

1

VGISVSTLQMYENGQRIPRDNIKIKLANFYGVTVQIIFFDSEQHEVC

47

VGISVSTLQMYENGQRIPRDNIKIKLANFYGVTVQIIFFDSEQHEVC

1

VGISVSTLQMYENGQRIPRDNIKIKLANFYGVTVQIIFFDSEQHEVC

47

19.1%

NZ_JH600294.1:

Lanthipeptides: allorf_051692_051835_lanthipeptide

3

ISVSTLQMYENGQRIPRDNIKIK

25

+S LQ ++N R+P D I +K

8

LSKHHLQCFDNISRMPSDRIHVK

30

18.4%

NZ_SBIS02000007.1:

Lanthipeptides: EPL00_RS13710_lanthipeptide

1

VGISVSTLQMYENGQRIPRDNIKIKLANFYGVTVQIIF

38

VG++ T+ E + P + KLA+ + ++ +F

1

VGVTRQTINAIERERYDPSIELAFKLADHFDCRIEDLF

38

allorf_022679_022864

Leader:

MKWELLVSLGVTLGG

Core:

FNTRCPTLGCSLGTVDILRKNRMAWFSHSIFFLKPHISSYQRTSYS

Core with dehydrations:

FNDhbRCysPDhbLGCysDhaLGDhbVDILRKNRMAWFDhaHDhaIFFLKPHIDhaDhaYQRDhbDhaYDha

antiSMASH-DB 4.0 matches for allorf_022679_022864

92.0%

NZ_CP036100.1and 2 others:

NZ_CP072062.1 Lanthipeptides: allorf_377462_377647_lanthipeptideNZ_QNRN01000010.1 Lanthipeptides: allorf_022679_022864_lanthipeptide

Lanthipeptides: allorf_397095_397280_lanthipeptide

1

FNTRCPTLGCSLGTVDILRKNRMAWFSHSIFFLKPHISSYQRTSYS

46

FNTRCPTLGCSLGTVDILRKNRMAWFSHSIFFLKPHISSYQRTSYS

5

FNTRCPTLGCSLGTVDILRKNRMAWFSHSIFFLKPHISSYQRTSYS

50

88.0%

NZ_JH792312.1:

Lanthipeptides: allorf_249460_249645_lanthipeptide

1

FNTRCPTLGCSLGTVDILRKNRMAWFSHSIFFLKPHISSYQRTSYS

46

FNTRCPT GCSLGTVDILR+NRMAWFSHSIFFLKPHISSYQRTSYS

5

FNTRCPTRGCSLGTVDILRRNRMAWFSHSIFFLKPHISSYQRTSYS

50

19.6%

NC_012489.1:

Lanthipeptides: allorf_4588962_4589087_lanthipeptide

5

CPTLGCSLGTVDILRKNRMA

24

C GC GT LR R+A

5

CIGCGCVEGTQRALRHERLA

24

with 3 more sequence matches

13.0%

NZ_BROP01000004.1:

Lanthipeptides: OPC11_RS12075_lanthipeptide

16

DILRKNRMAWFSHSI

30

D+L M WFS +

15

DLLGPAEMCWFSCGL

29

13.0%

NZ_CP102975.1and 1 other:

NZ_FMOE01000002.1 Lanthipeptides: BJT27_RS07810_lanthipeptide

Lanthipeptides: NW953_RS00175_lanthipeptide

2

NTRCPTLGCS

11

+CPTL CS

16

TAQCPTLACS

25

13.0%

NZ_JAMTCO010000001.1:

Lanthipeptides: allorf_486573_486749_lanthipeptide

4

RCPTLGCSLGTV

15

RCP+ CS+ T

20

RCPSDRCSVATA

31

MIBiG 4.0 matches for allorf_022679_022864

8.7%

BGC0000563.4 (AEA03263.1):

Lanthipeptide: venezuelin

1

FNTRCPTLGCS

11

NT C + C+

12

LNTVCIGISCA

22

allorf_027261_027326

Leader:

MQIHSYYLYIMIKIFFS

Core:

CKIS

Core with dehydrations:

CysKIDha

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.21 out of 30.25

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.71 out of 26.33

Confidence: medium

Additional weak hits (4):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 23.52 out of 33.73

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 22.23 out of 33.73

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 18.44 out of 30.11

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Core peptide predictions

DET63_RS17570 - Class II

Cleavage pHMM score: -0.80

RODEO score: 0

Monoisotopic mass: 3711.8 Da

Molecular weight: 3714.4 Da

Number of bridges: 3

DET63_RS17575 - Class II

Cleavage pHMM score: -6.70

RODEO score: 0

Monoisotopic mass: 4337.2 Da

Molecular weight: 4340.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4358.0 Da N = 1

DET63_RS17600 - Class II

Cleavage pHMM score: -6.20

RODEO score: 0

Monoisotopic mass: 5308.7 Da

Molecular weight: 5312.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 5330.0 Da N = 1; 5348.1 Da N = 2; 5366.1 Da N = 3; 5384.1 Da N = 4

allorf_022679_022864 - Class II

Cleavage pHMM score: -4.70

RODEO score: 0

Monoisotopic mass: 5166.7 Da

Molecular weight: 5169.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 5187.9 Da N = 1; 5206.0 Da N = 2; 5224.0 Da N = 3; 5242.0 Da N = 4; 5260.0 Da N = 5; 5278.0 Da N = 6; 5296.1 Da N = 7; 5314.1 Da N = 8; 5332.1 Da N = 9

allorf_027261_027326 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 431.2 Da

Molecular weight: 431.5 Da

Number of bridges: 1

Predicted core structure(s)

For candidate cluster 1, location 0 - 31935:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Leu - Tyr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

DET63_RS17530: Leu - Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (477..877): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSLAFDASIMEPLLFVGGELNAYGPTEATVCTTI
Nearest Stachelhaus code(s):: Leu DAMLVGAVCK (71% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1538..1944): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: LWRYFDSSVSESDIIMGGEVNEYGPTEATVSAMY
Nearest Stachelhaus code(s):: Lys DAEDIGTVSK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
ColR	Phenol-responsive regulator

NZ_QNRN01000010.1 - Region 2 - lanthipeptide-class-i

Download region GenBank file

Download region SVG

Location: 84,620 - 109,019 nt. (total: 24,400 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: protease

DET63_RS17950: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase dehydratase

DET63_RS17940: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

DET63_RS17945: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.74	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0000560.5	0.69	ribosomal	subtilomycin	Bacillus subtilis
BGC0000507.5	0.60	ribosomal	epicidin	Staphylococcus epidermidis
BGC0000543.5	0.59	ribosomal	pep5	Staphylococcus epidermidis
BGC0000540.5	0.57	ribosomal	paenibacillin	Paenibacillus polymyxa OSY-DF
BGC0000510.3	0.55	ribosomal	epilancin K7	Staphylococcus epidermidis
BGC0001289.5	0.52	ribosomal	penisin	Paenibacillus ehimensis
BGC0000509.4	0.49	ribosomal	epilancin 15x	Staphylococcus epidermidis
BGC0002323.2	0.48	ribosomal	nisin J	Staphylococcus capitis
BGC0000541.5	0.48	ribosomal	paenicidin A	Paenibacillus polymyxa

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.74	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0000560.5	0.69	ribosomal	subtilomycin	Bacillus subtilis
BGC0000507.5	0.60	ribosomal	epicidin	Staphylococcus epidermidis
BGC0000543.5	0.59	ribosomal	pep5	Staphylococcus epidermidis
BGC0000540.5	0.57	ribosomal	paenibacillin	Paenibacillus polymyxa OSY-DF
BGC0000510.3	0.55	ribosomal	epilancin K7	Staphylococcus epidermidis
BGC0001289.5	0.52	ribosomal	penisin	Paenibacillus ehimensis
BGC0000509.4	0.49	ribosomal	epilancin 15x	Staphylococcus epidermidis
BGC0002323.2	0.48	ribosomal	nisin J	Staphylococcus capitis
BGC0000541.5	0.48	ribosomal	paenicidin A	Paenibacillus polymyxa

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for DET63_RS17945

DET63_RS17955

Leader:

MNKELFDLDINKKMETPTEMTAQ

Core:

TWGTVVKVSKAICKTGTCICTTSCSNCK

Core with dehydrations:

DhbWGDhbVVKVDhaKAICysKDhbGDhbCysICysDhbDhbDhaCysDhaNCysK

antiSMASH-DB 4.0 matches for DET63_RS17955

100.0%

NZ_QNRN01000010.1:

Lanthipeptides: DET63_RS17955_lanthipeptide

1

TWGTVVKVSKAICKTGTCICTTSCSNCK

28

TWGTVVKVSKAICKTGTCICTTSCSNCK

1

TWGTVVKVSKAICKTGTCICTTSCSNCK

28

89.3%

NZ_MOOT01000054.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17680_lanthipeptide

Lanthipeptides: BK765_RS17685_lanthipeptide

1

TWGTVVKVSKAICKTGTCICTTSCSNCK

28

TW T+VKVSKA+CKTGTCICTTSCSNCK

1

TWTTIVKVSKAVCKTGTCICTTSCSNCK

28

82.1%

NZ_CP036100.1and 1 other:

NZ_CP072062.1 Lanthipeptides: J5V93_RS29680_lanthipeptide

Lanthipeptides: EXW47_RS29590_lanthipeptide

1

TWGTVVKVSKAICKTGTCICTTSCSNCK

28

TWG+++KVS+A+CKTGTCICTTSCSNCK

1

TWGSILKVSRAVCKTGTCICTTSCSNCK

28

with 17 more sequence matches

Only the first 10 are displayed here.

82.1%

NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17670_lanthipeptide

Lanthipeptides: ICC_RS25585_lanthipeptide

3

GTVVKVSKAICKTGTCICTTSCSNCK

28

GTVVKVSKAICKTGTCI T SC+NCK

1

GTVVKVSKAICKTGTCIGTISCTNCK

26

78.6%

NZ_CP036100.1and 5 others:

NZ_CP036100.1 Lanthipeptides: EXW47_RS29575_lanthipeptideNZ_CP036100.1 Lanthipeptides: EXW47_RS29570_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29670_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29665_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29660_lanthipeptide

Lanthipeptides: EXW47_RS29580_lanthipeptide

4

TVVKVSKAICKTGTCICTTSCSNCK

28

TV+K SKA CKTGTCICTTSCSNCK

2

TVIKFSKAFCKTGTCICTTSCSNCK

26

71.4%

NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17675_lanthipeptide

Lanthipeptides: ICC_RS37095_lanthipeptide

1

TWGTVVKVSKAICKTGTCICTTSCSNCK

28

TW +++KVS+A CK+GTC+CTTSCSNCK

1

TWTSILKVSRAPCKSGTCLCTTSCSNCK

28

66.7%

NZ_CP023711.1and 11 others:

NZ_CP034141.1 Lanthipeptides: EGM68_RS25880_lanthipeptideNZ_CP040829.1 Lanthipeptides: FGY93_RS26165_lanthipeptideNZ_CP049598.1 Lanthipeptides: G7L40_RS03920_lanthipeptideNZ_CP071875.1 Lanthipeptides: J4I02_RS05145_lanthipeptideNZ_CP086373.1 Lanthipeptides: LMH85_RS04795_lanthipeptideNZ_CP092831.1 Lanthipeptides: MLD56_RS04795_lanthipeptideNZ_CP102865.1 Lanthipeptides: NWS42_RS17805_lanthipeptideNZ_JAFJXZ010000012.1 Lanthipeptides: JYU28_RS03620_lanthipeptideNZ_JAKVDC010000009.1 Lanthipeptides: MKN04_RS18550_lanthipeptideNZ_JWJJ01000002.1 Lanthipeptides: U707_RS25810_lanthipeptideNZ_POVT01000017.1 Lanthipeptides: C1T20_RS25545_lanthipeptide

Lanthipeptides: CPY53_RS19275_lanthipeptide

4

TVVKVSKAICKTGTCICTTSCSNCK

28

T +KVSKA+CKT TCICT SCSNCK

6

TTIKVSKAVCKTLTCICTGSCSNCK

30

60.7%

NZ_KB976165.1:

Lanthipeptides: ICC_RS25590_lanthipeptide

3

GTVVKVSKAICKTGTCICTTSCSNCK

28

T + VS+++CKT TCICT SCSNCK

1

PTTILVSRSVCKTLTCICTISCSNCK

26

60.7%

NZ_MOOT01000054.1:

Lanthipeptides: BK765_RS17665_lanthipeptide

3

GTVVKVSKAICKTGTCICTTSCSNCK

28

T + VS+++CKT TCICT SCSNCK

1

PTTIFVSRSVCKTLTCICTISCSNCK

26

57.1%

NZ_QNRN01000010.1:

Lanthipeptides: DET63_RS17960_lanthipeptide

6

VKVSKAICKTGTCICTTSCSNCK

28

+ +S+ ICKT TC+CTTSCSNCK

3

ILISRTICKTLTCVCTTSCSNCK

25

50.0%

NZ_BDDH01000005.1and 2 others:

NZ_CP073265.1 Lanthipeptides: KCX77_RS01160_lanthipeptideNZ_JALAUD010000002.1 Lanthipeptides: MOE28_RS05435_lanthipeptide

Lanthipeptides: A7P71_RS15855_lanthipeptide

1

TWGTVVKV---SKAICKTGTCICTT-SCSNCK

28

TW T+ K S C+T TC C+ SCSNCK

1

TWATIGKTIVQSVKKCRTFTCGCSLGSCSNCK

32

46.9%

NC_014976.1and 6 others:

NZ_CP029461.1 Lanthipeptides: DJ572_RS00745_lanthipeptideNZ_CP031675.1 Lanthipeptides: DYS67_RS00910_lanthipeptideNZ_CP045824.1 Lanthipeptides: GII78_RS01205_lanthipeptideNZ_CP046591.1 Lanthipeptides: GO004_RS12840_lanthipeptideNZ_CP049924.1 Lanthipeptides: G4G26_RS01205_lanthipeptideNZ_CP095736.1 Lanthipeptides: MX663_RS01330_lanthipeptide

Lanthipeptides: BSN5_RS12800_lanthipeptide

1

TWGTVVKV---SKAICKTGTCICTT-SCSNCK

28

TW T+ K S C+T TC C+ SCSNC

1

TWATIGKTIVQSVKKCRTFTCGCSLGSCSNCN

32

36.7%

NZ_JAELVP010000007.1and 1 other:

NZ_LT963438.1 Lanthipeptides: DG016_RS11145_lanthipeptide

Lanthipeptides: JF558_RS03770_lanthipeptide

1

TWGTVVKVSKAICKTGTCICTTSC--SNCK

28

+ GT +K +K C T + T SC S+C+

1

SLGTAIKATKNACPKVTRLVTVSCQKSDCQ

30

DET63_RS17960

Leader:

MNKELFDLDINKKMETSTEMTAQTV

Core:

PTILISRTICKTLTCVCTTSCSNCK

Core with dehydrations:

PDhbILIDhaRDhbICysKDhbLDhbCysVCysDhbDhbDhaCysDhaNCysK

antiSMASH-DB 4.0 matches for DET63_RS17960

100.0%

NZ_QNRN01000010.1:

Lanthipeptides: DET63_RS17960_lanthipeptide

1

PTILISRTICKTLTCVCTTSCSNCK

25

PTILISRTICKTLTCVCTTSCSNCK

1

PTILISRTICKTLTCVCTTSCSNCK

25

73.1%

NZ_KB976165.1:

Lanthipeptides: ICC_RS25590_lanthipeptide

2

TILISRTICKTLTCVCTTSCSNCK

25

TIL+SR++CKTLTC+CT SCSNCK

3

TILVSRSVCKTLTCICTISCSNCK

26

69.2%

NZ_MOOT01000054.1:

Lanthipeptides: BK765_RS17665_lanthipeptide

2

TILISRTICKTLTCVCTTSCSNCK

25

TI +SR++CKTLTC+CT SCSNCK

3

TIFVSRSVCKTLTCICTISCSNCK

26

with 13 more sequence matches

Only the first 10 are displayed here.

61.5%

NZ_CP036100.1and 5 others:

NZ_CP036100.1 Lanthipeptides: EXW47_RS29575_lanthipeptideNZ_CP036100.1 Lanthipeptides: EXW47_RS29570_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29670_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29665_lanthipeptideNZ_CP072062.1 Lanthipeptides: J5V93_RS29660_lanthipeptide

Lanthipeptides: EXW47_RS29580_lanthipeptide

3

ILISRTICKTLTCVCTTSCSNCK

25

I S+ CKT TC+CTTSCSNCK

4

IKFSKAFCKTGTCICTTSCSNCK

26

57.1%

NZ_CP036100.1and 1 other:

NZ_CP072062.1 Lanthipeptides: J5V93_RS29680_lanthipeptide

Lanthipeptides: EXW47_RS29590_lanthipeptide

5

ISRTICKTLTCVCTTSCSNCK

25

+SR +CKT TC+CTTSCSNCK

8

VSRAVCKTGTCICTTSCSNCK

28

57.1%

NZ_QNRN01000010.1:

Lanthipeptides: DET63_RS17955_lanthipeptide

3

ILISRTICKTLTCVCTTSCSNCK

25

+ +S+ ICKT TC+CTTSCSNCK

6

VKVSKAICKTGTCICTTSCSNCK

28

56.7%

NZ_CP023711.1and 11 others:

NZ_CP034141.1 Lanthipeptides: EGM68_RS25880_lanthipeptideNZ_CP040829.1 Lanthipeptides: FGY93_RS26165_lanthipeptideNZ_CP049598.1 Lanthipeptides: G7L40_RS03920_lanthipeptideNZ_CP071875.1 Lanthipeptides: J4I02_RS05145_lanthipeptideNZ_CP086373.1 Lanthipeptides: LMH85_RS04795_lanthipeptideNZ_CP092831.1 Lanthipeptides: MLD56_RS04795_lanthipeptideNZ_CP102865.1 Lanthipeptides: NWS42_RS17805_lanthipeptideNZ_JAFJXZ010000012.1 Lanthipeptides: JYU28_RS03620_lanthipeptideNZ_JAKVDC010000009.1 Lanthipeptides: MKN04_RS18550_lanthipeptideNZ_JWJJ01000002.1 Lanthipeptides: U707_RS25810_lanthipeptideNZ_POVT01000017.1 Lanthipeptides: C1T20_RS25545_lanthipeptide

Lanthipeptides: CPY53_RS19275_lanthipeptide

2

TILISRTICKTLTCVCTTSCSNCK

25

TI +S+ +CKTLTC+CT SCSNCK

7

TIKVSKAVCKTLTCICTGSCSNCK

30

53.6%

NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17675_lanthipeptide

Lanthipeptides: ICC_RS37095_lanthipeptide

3

ILISRTICKTLTCVCTTSCSNCK

25

+ +SR CK+ TC+CTTSCSNCK

6

LKVSRAPCKSGTCLCTTSCSNCK

28

53.6%

NZ_MOOT01000054.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17680_lanthipeptide

Lanthipeptides: BK765_RS17685_lanthipeptide

3

ILISRTICKTLTCVCTTSCSNCK

25

+ +S+ +CKT TC+CTTSCSNCK

6

VKVSKAVCKTGTCICTTSCSNCK

28

50.0%

NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17670_lanthipeptide

Lanthipeptides: ICC_RS25585_lanthipeptide

3

ILISRTICKTLTCVCTTSCSNCK

25

+ +S+ ICKT TC+ T SC+NCK

4

VKVSKAICKTGTCIGTISCTNCK

26

43.8%

NZ_BDDH01000005.1and 2 others:

NZ_CP073265.1 Lanthipeptides: KCX77_RS01160_lanthipeptideNZ_JALAUD010000002.1 Lanthipeptides: MOE28_RS05435_lanthipeptide

Lanthipeptides: A7P71_RS15855_lanthipeptide

1

PTILISRTICKTLTCVCT-TSCSNCK

25

TI+ S C+T TC C+ SCSNCK

7

KTIVQSVKKCRTFTCGCSLGSCSNCK

32

40.6%

NC_014976.1and 6 others:

NZ_CP029461.1 Lanthipeptides: DJ572_RS00745_lanthipeptideNZ_CP031675.1 Lanthipeptides: DYS67_RS00910_lanthipeptideNZ_CP045824.1 Lanthipeptides: GII78_RS01205_lanthipeptideNZ_CP046591.1 Lanthipeptides: GO004_RS12840_lanthipeptideNZ_CP049924.1 Lanthipeptides: G4G26_RS01205_lanthipeptideNZ_CP095736.1 Lanthipeptides: MX663_RS01330_lanthipeptide

Lanthipeptides: BSN5_RS12800_lanthipeptide

1

PTILISRTICKTLTCVCT-TSCSNC

24

TI+ S C+T TC C+ SCSNC

7

KTIVQSVKKCRTFTCGCSLGSCSNC

31

26.7%

NZ_FNJN01000003.1and 1 other:

NZ_FNJN01000003.1 Lanthipeptides: BLS25_RS16855_lanthipeptide

Lanthipeptides: BLS25_RS16855_lanthipeptide

1

PTILISRTICKTL

13

P I +SR ICKT+

3

PVIRLSRIICKTI

15

MIBiG 4.0 matches for DET63_RS17960

21.4%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

12

TLTCVCTTSCS

22

TLT C SC+

18

TLTVECMPSCN

28

20.0%

BGC0001155.5 (AGY49587.1):

Thiopeptide: GE2270

14

TCVCTTSCS

22

CVC CS

3

NCVCGFCCS

11

16.7%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

12

TLTCVCTTSCS

22

TLT C +C+

20

TLTKECQPNCN

30

with 1 more sequence matches

12.0%

BGC0000563.4 (AEA03263.1):

Lanthipeptide: venezuelin

7

RTICKTLTC

15

T+C ++C

13

NTVCIGISC

21

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 23.70 out of 29.25

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 25.84 out of 29.38

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.50 out of 20.97

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.49 out of 24.78

Confidence: medium

Additional weak hits (5):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 24.68 out of 32.74

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 25.92 out of 31.73

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.90 out of 25.88

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 24.41 out of 30.97

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 23.27 out of 29.65

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

DET63_RS17955 - Class I

Cleavage pHMM score: 10.10

RODEO score: 26

Monoisotopic mass: 2759.4 Da

Molecular weight: 2761.3 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 2779.3 Da N = 1; 2797.3 Da N = 2; 2815.4 Da N = 3; 2833.4 Da N = 4

DET63_RS17960 - Class I

Cleavage pHMM score: 12.40

RODEO score: 26

Monoisotopic mass: 2526.3 Da

Molecular weight: 2528.1 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 2546.1 Da N = 1; 2564.1 Da N = 2; 2582.1 Da N = 3; 2600.2 Da N = 4

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 146 - 238; Score: 55.3

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
FuR variant 1	Ferric Uptake Regulator
LexA variant 2	Repressor of DNA damage response
SypG	Regulator of biofilm formation and host colonization

NZ_QNRN01000010.1 - Region 3 - RiPP-like

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Location: 208,201 - 219,439 nt. (total: 11,239 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001157.6	0.30	ribosomal	bottromycin A2	Streptomyces scabiei 87.22
BGC0000468.5	0.30	ribosomal	bottromycin A2	Streptomyces bottropensis DSM40262
BGC0000470.6	0.30	ribosomal	bottromycin D	Streptomyces sp. WMMB272
BGC0000469.6	0.29	ribosomal	bottromycin A2	Streptomyces sp. BC16019
BGC0001418.4	0.27	NRPS, PKS	myxochromide D	Hyalangium minutum
BGC0001419.4	0.27	NRPS, PKS	myxochromide D	Hyalangium minutum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001157.6	0.30	ribosomal	bottromycin A2	Streptomyces scabiei 87.22
BGC0000468.5	0.30	ribosomal	bottromycin A2	Streptomyces bottropensis DSM40262
BGC0000470.6	0.30	ribosomal	bottromycin D	Streptomyces sp. WMMB272
BGC0000469.6	0.29	ribosomal	bottromycin A2	Streptomyces sp. BC16019
BGC0001418.4	0.27	NRPS, PKS	myxochromide D	Hyalangium minutum
BGC0001419.4	0.27	NRPS, PKS	myxochromide D	Hyalangium minutum

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 22.96 out of 29.25

Confidence: medium

Additional weak hits (1):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 25.50 out of 39.62

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
SypG	Regulator of biofilm formation and host colonization

NZ_QNRN01000012.1 - Region 1 - azole-containing-RiPP

Download region GenBank file

Download region SVG

Location: 146,048 - 179,772 nt. (total: 33,725 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

DET63_RS20745: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Hydrolases: hydrolase phosphatase

DET63_RS20795: inositol monophosphatase

smcogs: SMCOG1207: inositol monophosphatase

DET63_RS20800: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001174.4	0.47	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0000607.5	0.45	ribosomal	micrococcin P1	Macrococcus caseolyticus
BGC0001173.5	0.45	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000569.5	0.44	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0001146.5	0.43	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0000566.5	0.43	ribosomal	streptolysin S	Streptococcus pyogenes M1 GAS
BGC0001535.3	0.43	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0000564.5	0.43	ribosomal	clostridiolysin S	Clostridium botulinum A str. ATCC 3502
BGC0001170.5	0.42	ribosomal	clostridiolysin S	Clostridium sporogenes ATCC 15579
BGC0002700.2	0.42	ribosomal	bacinapeptin	Bacillus nakamurai

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001174.4	0.47	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0000607.5	0.45	ribosomal	micrococcin P1	Macrococcus caseolyticus
BGC0001173.5	0.45	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000569.5	0.44	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0001146.5	0.43	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0000566.5	0.43	ribosomal	streptolysin S	Streptococcus pyogenes M1 GAS
BGC0001535.3	0.43	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0000564.5	0.43	ribosomal	clostridiolysin S	Clostridium botulinum A str. ATCC 3502
BGC0001170.5	0.42	ribosomal	clostridiolysin S	Clostridium sporogenes ATCC 15579
BGC0002700.2	0.42	ribosomal	bacinapeptin	Bacillus nakamurai

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.57 out of 18.57

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.74 out of 26.06

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 22.91 out of 24.78

Confidence: strong

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.68 out of 24.78

Confidence: medium

Additional weak hits (2):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 30.06 out of 39.62

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 20.85 out of 30.22

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

RREFinder

TFBS Finder

Azole RiPPs

RRE predictions

: RRE type: Heterocycloanthracin_C_RRE; Location: 0 - 100; Score: 181.4; RRE type: Thiopeptide_F_RRE; Location: 9 - 89; Score: 41.5

TFBS definitions

Regulator	Description
LexA variant 2	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes
ToxT	Regulator of MSHA biosynthesis

Core peptide predictions

No core peptides found.

NZ_QNRN01000013.1 - Region 1 - NRP-metallophore,NRPS

Download region GenBank file

Download region SVG

Location: 143,826 - 195,586 nt. (total: 51,761 nt) Show pHMM detection rules used

Region on contig edge.

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxygenase

DET63_RS21750: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

DET63_RS21755: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

DET63_RS21760: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

DET63_RS21810: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

DET63_RS21870: Antibiotic biosynthesis monooxygenase

smcogs: SMCOG1258: Antibiotic biosynthesis monooxygenase

DET63_RS21920: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

Transferases: PPTase

DET63_RS21845: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: hydrolase

DET63_RS21825: isochorismatase

smcogs: SMCOG1027: isochorismatase

Lyases: hydratase

DET63_RS21770: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Isomerases: isomerase

DET63_RS21815: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

DET63_RS21820: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

DET63_RS21830: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

DET63_RS21885: Pseudouridine synthase

smcogs: SMCOG1154: Pseudouridine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001185.5	1.05	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0000309.5	0.92	NRPS	bacillibactin	Bacillus subtilis subsp. subtilis str. 168
BGC0000401.5	0.88	NRPS	paenibactin	Paenibacillus elgii B69
BGC0002075.3	0.86	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000343.5	0.85	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001132.4	0.77	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.74	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002695.2	0.73	NRPS	bacillibactin, bacillibactin E, bacillibactin F	Bacillus sp. WMMC1349

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001185.5	0.80	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0000309.5	0.76	NRPS	bacillibactin	Bacillus subtilis subsp. subtilis str. 168
BGC0000401.5	0.75	NRPS	paenibactin	Paenibacillus elgii B69
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000343.5	0.74	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002695.2	0.69	NRPS	bacillibactin, bacillibactin E, bacillibactin F	Bacillus sp. WMMC1349

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 24.83 out of 27.78

Confidence: strong

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.00 out of 21.55

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.57 out of 25.31

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.16 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.72 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.71 out of 27.78

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.32 out of 27.78

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 25.11 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 25.40 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.10 out of 29.38

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 25.19 out of 30.25

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.91 out of 28.19

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.17 out of 24.78

Confidence: medium

Additional weak hits (12):

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.16 out of 27.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.73 out of 30.25

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 24.85 out of 36.54

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.92 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 25.52 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.17 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.24 out of 32.74

Confidence: weak

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 15.72 out of 18.46

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.93 out of 32.74

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.53 out of 30.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.52 out of 30.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.54 out of 30.25

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 143825 - 195586:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

DET63_RS21820: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SISLYPMSSPGGALQVGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz PMPAQGVVNK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

DET63_RS21830: Gly - Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (465..865): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FAMTFDISALELQALVGGETNLYGPTETTIWSTF
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1525..1938): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LATHFDFSVWEGNQVFGGEINMYGITETTVHVSY
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ArcA	Repressor of carbon oxidation
CatR	H2O2-responsive repressor
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ColR	Phenol-responsive regulator
FuR variant 1	Ferric Uptake Regulator
LexA variant 2	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes
ZuR variant 1	Zinc-responsive repressor

NZ_QNRN01000015.1 - Region 1 - NRPS-like

Download region GenBank file

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Location: 77,694 - 121,275 nt. (total: 43,582 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase

DET63_RS23420: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Transferases: phosphorylase phosphotransferase

DET63_RS23385: thiamine-phosphate pyrophosphorylase

smcogs: SMCOG1280: thiamine-phosphate pyrophosphorylase

DET63_RS23410: Glucose-specific phosphotransferase enzyme IIA

smcogs: SMCOG1145: Glucose-specific phosphotransferase enzyme IIA

Hydrolases: glucosidase

DET63_RS23415: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

Ligases: ligase

DET63_RS23370: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.60	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.60	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.60	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.60	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.60	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.60	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.60	NRPS	epichloenin A	Epichloe festucae

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 24.83 out of 27.78

Confidence: strong

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 22.91 out of 25.88

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.55 out of 25.88

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.96 out of 20.97

Confidence: medium

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 26.68 out of 32.74

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 25.99 out of 29.38

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.81 out of 20.97

Confidence: medium

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.69 out of 21.55

Confidence: strong

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 22.93 out of 27.55

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 25.16 out of 28.19

Confidence: strong

Additional weak hits (3):

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.13 out of 25.88

Confidence: weak

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.11 out of 20.47

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 24.78 out of 31.73

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 77693 - 121275:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

DET63_RS23370: X

AMP-binding (224..621): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LWMPFDASVWEAALAIGGEHNVYGPTECTVVTTT
Nearest Stachelhaus code(s):: Leu DAWYLGNVVK (71% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ArcA	Repressor of carbon oxidation
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
FuR variant 1	Ferric Uptake Regulator
FuR variant 2	Ferric Uptake Regulator
GnfM	Regulator of nitrogen-fixation genes
PsrA	Oleic acid responsive regulator
ZuR variant 1	Zinc-responsive repressor

NZ_QNRN01000018.1 - Region 1 - NI-siderophore

Download region GenBank file

Download region SVG

Location: 47,408 - 79,125 nt. (total: 31,718 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase

DET63_RS25735: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: deacetylase

DET63_RS25670: polysaccharide deacetylase

smcogs: SMCOG1235: polysaccharide deacetylase

Ligases: ligase synthase

DET63_RS25645: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

DET63_RS25700: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000942.4	0.87	other (other)	petrobactin	Bacillus anthracis str. Ames
BGC0002469.2	0.54	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0000295.5	0.53	NRPS	acinetoferrin	Acinetobacter haemolyticus
BGC0000941.3	0.50	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002692.2	0.50	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0001531.5	0.49	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002683.2	0.49	NRPS	schizokinen	Nostoc sp. PCC 7120 = FACHB-418
BGC0002471.3	0.49	other (other)	baumannoferrin A, baumannoferrin B	Acinetobacter baumannii AYE
BGC0002694.2	0.48	other (other)	roseobactin	Phaeobacter inhibens 2.10
BGC0002633.2	0.48	other (other)	schizokinen	Leptolyngbya sp. PCC 7376

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000942.4	0.87	other (other)	petrobactin	Bacillus anthracis str. Ames
BGC0002469.2	0.54	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0000295.5	0.53	NRPS	acinetoferrin	Acinetobacter haemolyticus
BGC0000941.3	0.50	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002692.2	0.50	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0001531.5	0.49	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002683.2	0.49	NRPS	schizokinen	Nostoc sp. PCC 7120 = FACHB-418
BGC0002471.3	0.49	other (other)	baumannoferrin A, baumannoferrin B	Acinetobacter baumannii AYE
BGC0002694.2	0.48	other (other)	roseobactin	Phaeobacter inhibens 2.10
BGC0002633.2	0.48	other (other)	schizokinen	Leptolyngbya sp. PCC 7376

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 26.61 out of 29.38

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.55 out of 19.26

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.55 out of 19.26

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 21.06 out of 25.88

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.18 out of 28.19

Confidence: medium

Additional weak hits (2):

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 16.35 out of 20.47

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.12 out of 29.38

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
FuR variant 1	Ferric Uptake Regulator
PsrA	Oleic acid responsive regulator
ZuR variant 1	Zinc-responsive repressor

NZ_QNRN01000030.1 - Region 1 - azole-containing-RiPP

Download region GenBank file

Download region SVG

Location: 1 - 21,375 nt. (total: 21,375 nt) Show pHMM detection rules used

Region on contig edge.

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

Tailoring

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KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: dehydratase

DET63_RS29490: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001174.4	0.54	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0002311.3	0.49	ribosomal	cutimycin	Cutibacterium acnes KPA171202
BGC0001171.5	0.45	ribosomal	listeriolysin S	Listeria monocytogenes serotype 4b str. F2365
BGC0000607.5	0.45	ribosomal	micrococcin P1	Macrococcus caseolyticus
BGC0000569.5	0.45	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0001640.3	0.43	ribosomal	kocurin	Kocuria rosea
BGC0002013.4	0.42	ribosomal	curacozole	Streptomyces curacoi
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0000605.5	0.40	ribosomal	GE37468	Streptomyces sp. ATCC 55365
BGC0000612.4	0.38	ribosomal	thiocillin I	Bacillus cereus ATCC 14579

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001174.4	0.54	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0002311.3	0.49	ribosomal	cutimycin	Cutibacterium acnes KPA171202
BGC0001171.5	0.45	ribosomal	listeriolysin S	Listeria monocytogenes serotype 4b str. F2365
BGC0000607.5	0.45	ribosomal	micrococcin P1	Macrococcus caseolyticus
BGC0000569.5	0.45	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0001640.3	0.43	ribosomal	kocurin	Kocuria rosea
BGC0002013.4	0.42	ribosomal	curacozole	Streptomyces curacoi
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0000605.5	0.40	ribosomal	GE37468	Streptomyces sp. ATCC 55365
BGC0000612.4	0.38	ribosomal	thiocillin I	Bacillus cereus ATCC 14579

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.00 out of 27.78

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.08 out of 23.79

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.69 out of 24.78

Confidence: medium

Additional weak hits (2):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.73 out of 30.25

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 21.69 out of 30.11

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

TFBS Finder

Azole RiPPs

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
IolR	Regulator of the inositol catabolism
LexA variant 2	Repressor of DNA damage response

Core peptide predictions

No core peptides found.

NZ_QNRN01000031.1 - Region 1 - cyclic-lactone-autoinducer

Download region GenBank file

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Location: 1 - 14,383 nt. (total: 14,383 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: formyltransferase

DET63_RS29605: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

Hydrolases: hydrolase

DET63_RS29615: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002579.2	0.45	ribosomal	carnobacteriocin XY	Carnobacterium maltaromaticum
BGC0000540.5	0.41	ribosomal	paenibacillin	Paenibacillus polymyxa OSY-DF
BGC0002667.2	0.37	ribosomal	estericin A	Clostridium estertheticum
BGC0002669.2	0.36	ribosomal	TVA-YJ-2	Streptomyces sp. NRRL S-87
BGC0000494.5	0.34	ribosomal	uberolysin	Streptococcus uberis
BGC0000624.3	0.30	ribosomal	salivaricin CRL1328 α peptide, salivaricin CRL1328 β peptide	Lactobacillus salivarius
BGC0002282.2	0.29	other (other)	formycin A	Streptomyces kaniharaensis
BGC0002040.3	0.28	other (other)	coformycin, formycin A	Streptomyces kaniharaensis
BGC0002146.2	0.28	NRPS	FR901483	Cladobotryum sp.
BGC0002039.3	0.26	other (other)	coformycin, formycin A	Nocardia interforma ATCC 21072

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002579.2	0.45	ribosomal	carnobacteriocin XY	Carnobacterium maltaromaticum
BGC0000540.5	0.41	ribosomal	paenibacillin	Paenibacillus polymyxa OSY-DF
BGC0002667.2	0.37	ribosomal	estericin A	Clostridium estertheticum
BGC0002669.2	0.36	ribosomal	TVA-YJ-2	Streptomyces sp. NRRL S-87
BGC0000494.5	0.34	ribosomal	uberolysin	Streptococcus uberis
BGC0000624.3	0.30	ribosomal	salivaricin CRL1328 α peptide, salivaricin CRL1328 β peptide	Lactobacillus salivarius
BGC0002282.2	0.29	other (other)	formycin A	Streptomyces kaniharaensis
BGC0002040.3	0.28	other (other)	coformycin, formycin A	Streptomyces kaniharaensis
BGC0002146.2	0.28	NRPS	FR901483	Cladobotryum sp.
BGC0002039.3	0.26	other (other)	coformycin, formycin A	Nocardia interforma ATCC 21072

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.98 out of 28.19

Confidence: medium

TFBS Finder

TFBS definitions

Regulator	Description
ZuR variant 1	Zinc-responsive repressor