LOCUS       NZ_CP015163            10393 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Amycolatopsis albispora strain WP1 chromosome, complete genome.
ACCESSION   NZ_CP015163
VERSION     NZ_CP015163.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis albispora
  ORGANISM  Amycolatopsis albispora
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9121443)
  AUTHORS   Wang,H., Chen,S. and Wu,Q.
  TITLE     Complete genome sequence and analysis of deep-sea sediment isolate,
            Amycolatopsis sp. WP1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9121443)
  AUTHORS   Wang,H., Chen,S. and Wu,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2016) Zhejiang University of Technology, College
            of Pharmaceutical Science, Chaowang Road No.18, Hangzhou 310014,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera assembler v. unspecified
            Genome Coverage                   :: 138.17X
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 04:57:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,537
            CDSs (total)                      :: 8,472
            Genes (coding)                    :: 8,394
            CDSs (with protein)               :: 8,394
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 26 of 78
            Pseudo Genes (incomplete)         :: 54 of 78
            Pseudo Genes (internal stop)      :: 6 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:20:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5638944
            Orig. end                         :: 5649337
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015163.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="24"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5393](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5393)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(963..3764)
                     /locus_tag="A4R43_RS26560"
                     /old_locus_tag="A4R43_26265"
     CDS             complement(963..3764)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416991.1"
                     /locus_tag="A4R43_RS26560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_26265"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_236808294.1"
                     /transl_table=11
                     /translation="MSEGDLGSHQAWFQLLGPVQLLAGSRPVPVGGPGVRGLLALLALH
                     ANKVVPLDDIIDALWGHDPPATARTIVHGNVSHLRRVLRSIQGDSGDGAQIHTRPPGYQ
                     LTVDPSQIDVHRARALLEYATAAGAERRAAVLAEALALWKGPVLSGVPDSVHAPELEDL
                     RLAVHGARVDADLELGRHAELISELTTMVRENPLAERTIGQLMRALYHAGRRADALAVY
                     RRAARHVVGTLGIDPGAELRELHDRILRDDLADPSSPKGPPPRLVRVNPAQLPAAVPRF
                     AGRADELAWLDGLVEEARQGASMVGVVTGAPGVGKSALAISWAHRRAADFPDGVLFATL
                     RGFDPEHPPLAPGEVVTQFLLGLGVRPAELPEKPHERIALYRSLLADATVLVVLDDARS
                     AEQVRPLLPPAPGSVAVVTSRARLDGLVVASAARLRALESMPPADAVRLIEELAGPGDY
                     GKLAELCAYLPLALRIAGARLVVSPQWTVEDLVAELADERTRLAALDVAGVDVAETSVR
                     AALDVSHRGLPEPVGALFRLLGAFTGPSIGPHLAAALGGTGVAEARRRLRTLAAYHLLT
                     EVGRDVFAPHDLVRLYLRGLAETELAATEREAVLRRSVRYYQAAADRARRRLLRIVDSL
                     DFAAALPEAELPEFADFDDALDWFALEWPNLLETCEAAYRAGRYEDAWQLARVVHTYRV
                     VRPLRDEWTGLVELGLAAAKASGNPLAHCWMLISRCAIALTFGIEGGGLTDAEHALALA
                     AEVGDKRLLVSANIHVGAALSSSGRYDEAIDRLRQAIEETERTGDPALRGQALNNCAEA
                     EKLSGRYAEAIEHQLGSLAIDRELGDDSYAVVSLNNLAELHLGTGELGEAERYARQAIE
                     LTTSRGFVLQEGVSRAALGRVLRARGDRVQAREQLELAEERFRQAGAPWPAELGAELAA
                     VAE"
     misc_feature    4043..4063
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 21.9"
                     /tool="antismash"
     misc_feature    4050..4065
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.5"
                     /tool="antismash"
     misc_feature    complement(4052..4065)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 18.35"
                     /tool="antismash"
     misc_feature    complement(4052..4066)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.45"
                     /tool="antismash"
     misc_feature    4053..4066
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.58"
                     /tool="antismash"
     misc_feature    complement(4053..4068)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.73"
                     /tool="antismash"
     gene            complement(4061..4966)
                     /gene="thpD"
                     /locus_tag="A4R43_RS26565"
                     /old_locus_tag="A4R43_26270"
     CDS             complement(4061..4966)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="A4R43_RS26565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_26270"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_113694799.1"
                     /sec_met_domain="PhyH (E-value: 3.8e-35, bitscore: 121.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLMDTRIGDAYPTRLPAADAGAIDRLDPTVWGGEADGPIDAATL
                     ASHDAKGYSVVEGLLSPAEVQTYWQELVRLSGDEELKADERVVTEKSSGEVRSIFEVHR
                     ISDLIAELVRDPRVLDRARQILGSEVYVHQSRVNYMPGFRGNGFYWHSDFETWHAEDGM
                     PVPRAVSCSIALTDNYPFNGGLMVMPGSQRTFVQCAGRTPEDNYKSSLKEQKIGVPSED
                     EIAKLAAEHGIEQFTGAAGSALWFDSNIMHGSGNNITPYPRSNIFLVFNSVDNAVVEPF
                     AAEKRRPEFIAARDFTPVRR"
     gene            complement(5001..5393)
                     /locus_tag="A4R43_RS26570"
                     /old_locus_tag="A4R43_26275"
     CDS             complement(5001..5393)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084077.1"
                     /locus_tag="A4R43_RS26570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_26275"
                     /product="ectoine synthase"
                     /protein_id="WP_113694800.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.7e-53, bitscore:
                     178.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTLDEITDTDADIKTPNWRSKRIILAKEKVGFSVHETTLYAG
                     TVNDFWYANHIEAVFVYEGEGELVNKATGERHQLKPGSLYLLNDHDKHQVLPKTDMKTV
                     CVFNPPVTGREVHDENGVYPLVTEES"
     gene            complement(5414..6667)
                     /gene="ectB"
                     /locus_tag="A4R43_RS26575"
                     /old_locus_tag="A4R43_26280"
     CDS             complement(5414..6667)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 4.1e-73.
                     Score: 238.3. Matches aSDomain:
                     nrpspksdomains_A4R43_RS26575_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230319.1"
                     /locus_tag="A4R43_RS26575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_26280"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_113697896.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.3e-74, bitscore:
                     247.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIFSKLESEVRSYSRGWPVVFDRAQGSMLYDENGKGYLDFFAGA
                     GALNYGHNNPALKQALIDYIARDGVTHALDMFTVAKRDFLETLQEKILGPRELDYKVVF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHA
                     TPMPYDKYFDGAYPDFLYFERLLEDSGSGLNEPAAVIVEALQGEGGINAARLEWLKGLS
                     DLCERHNILLILDDVQMGCGRTGPFFSFEDAGIKPDIVCLSKSIGGYGLPLALTLIKPE
                     LDVWEPGEHNGTFRGINPAFVTATEALRVYWSDDKLEQSTRAKGERIGSVFDEIVQAHP
                     EANLVAKGRGLARGIEFASGELAGSVCAEAFDRGLLMETSGPDGEVMKLLPALTINDAE
                     LEQGLEIIGESVRAVLTK"
     aSDomain        complement(5594..6604)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R43_RS26575_Aminotran_3.1"
                     /evalue="4.10E-73"
                     /label="A4R43_RS26575_Aminotran_3.1"
                     /locus_tag="A4R43_RS26575"
                     /protein_end="358"
                     /protein_start="21"
                     /score="238.3"
                     /tool="antismash"
                     /translation="FDRAQGSMLYDENGKGYLDFFAGAGALNYGHNNPALKQALIDYIA
                     RDGVTHALDMFTVAKRDFLETLQEKILGPRELDYKVVFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHATPMPYDKYFDGAYPDFLYFER
                     LLEDSGSGLNEPAAVIVEALQGEGGINAARLEWLKGLSDLCERHNILLILDDVQMGCGR
                     TGPFFSFEDAGIKPDIVCLSKSIGGYGLPLALTLIKPELDVWEPGEHNGTFRGINPAFV
                     TATEALRVYWSDDKLEQSTRAKGERIGSVFDEIVQAHPEANLVAKGRGLARGIEFA"
     gene            complement(6722..7222)
                     /gene="ectA"
                     /locus_tag="A4R43_RS26580"
                     /old_locus_tag="A4R43_26285"
     CDS             complement(6722..7222)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669882.1"
                     /locus_tag="A4R43_RS26580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_26285"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_113694801.1"
                     /transl_table=11
                     /translation="MSGKHLIESPTKADGAELWRIARDSQKLDLNSPYAYLLWCRDFAD
                     TSVVARSGDAAVGFVIGYRKPSDPETALVWQVAVDASQRGKGLAGALLDALFDRLVGQG
                     VRYLETTITPDNQASIKLFESFAKRWEADLESAELFSSDDFPEDNVVHEPEDLYRIGPL
                     THR"
     misc_feature    7325..7338
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.26"
                     /tool="antismash"
     misc_feature    complement(7413..7433)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.32"
                     /tool="antismash"
     gene            complement(7503..8039)
                     /locus_tag="A4R43_RS26585"
                     /old_locus_tag="A4R43_26290"
     CDS             complement(7503..8039)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638401.1"
                     /locus_tag="A4R43_RS26585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_26290"
                     /product="DinB family protein"
                     /protein_id="WP_162788603.1"
                     /transl_table=11
                     /translation="MSNSSSKEMADPVERAWPETRADDELTLLWGFLDFLRATAVNKVA
                     GLSRAEAAGTPFPASPEMNALGVIRHLTAVERWWLTIVGGGSELPDLWGDTGDHDFLFR
                     LGKEHSPAELVAAYQEEWRISRAALAGLSADDPVRRPGEDKTVRWVLTHLVQETARHVG
                     HLDVLREFADGQVGE"
     misc_feature    complement(8021..8036)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.63"
                     /tool="antismash"
     gene            complement(8052..9287)
                     /locus_tag="A4R43_RS26590"
                     /old_locus_tag="A4R43_26295"
     CDS             complement(8052..9287)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084084.1"
                     /locus_tag="A4R43_RS26590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_26295"
                     /product="DUF445 domain-containing protein"
                     /protein_id="WP_113694803.1"
                     /transl_table=11
                     /translation="MSGIIDDFARNWPLYCSIPLIAALIGYTTKLVAIRMMFQPVEFVG
                     IKPFLGWQGVVPKRAARMAGIACDTMTQQLIKPGDVLNRLDPERIAKEIEKPLLAGVEE
                     IVRVVAAEYQPGLWESLPTRVQRMVITRVQAEAPRMVASIMDGVKQDVDSVFDLKDMVV
                     TSLVKDKRLLNRIFQEAGDKEFKFIARSGIVFGGLIGVIQMGAWILFKAPLIMPLFGLF
                     TGWFTDWLALKMIFRPQQPKRYLGLFEWQGLFLKRRAEVSEAYGALIAKEIITPHNVIE
                     AVLRGPASDKVLALVQRQVDAELAKHTSLARPLVVMAVGSKRYQAMKLRISELIMARLP
                     ETMTYVEDYAEDAMDIRNLLVTKMKQLSPAEFEGLLRPAFQQDEWILIATGALLGFAVG
                     EAQVLLLEHFAA"
     gene            complement(9325..10101)
                     /locus_tag="A4R43_RS26595"
                     /old_locus_tag="A4R43_26300"
     CDS             complement(9325..10101)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230325.1"
                     /locus_tag="A4R43_RS26595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_26300"
                     /product="Abi-alpha family protein"
                     /protein_id="WP_236808295.1"
                     /transl_table=11
                     /translation="MGAPENFAERAGKLASIAARAGYSLGKRLPGADAAERGLRSLERL
                     ALTELRRRLEEVDDPYLAALSAASAMNVGGNGRHVQPLNGEIRGTVVVMPSSESVEPLR
                     AAMAELLNRSIGFGRERAREYLYAIILRQLTPDEARILSALSDGAPFPLIDVTERNGLG
                     GAGRVVLRNASTVGKAAGVTLLDQVPAYVTRLIGLGLAEPDEEVPELETQYEILLTDDA
                     VRAAENSVKRVKFVRRTVHISRLGKEFWAACDPARS"
     misc_feature    10135..10149
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.76"
                     /tool="antismash"
ORIGIN
        1 gtgaggacgt gctgcgcagc atgaccaccg agcaggcgcg ggtgctgcgg agcagcttgc
       61 gcgagtcgcg cgaggaggtc gggtacgaga cggtgtcggg caggctcgac ggcgtgcgca
      121 cccggcggcg gatcttctac ctggaacgcg aatcgggcgg cacggtgcag ggcgcggtgg
      181 cgccggacct gctggacgag atcaagctga acctggatcg gccggtgacc gcgcggctgc
      241 gcgtggtccg caccaccacg atcgacggcc gccggggccg accggtctac gaactgctcg
      301 agatcacccc ggaaaccagc ctcttctgac gcggaccctc cgctcgcgcg ccgcggccag
      361 aacacgaatg ggcttatggg gcggatttcg ccccgcgccg aggcggcgtc gaaggtcgcc
      421 cgcagggtgt gcgaaagcag cggcagcgcg tcctgttcgt gcgcggcgcc cgcctcgccg
      481 ggcgtgccag gggttacctc gcggatgagc agttcgacca gcccgccgtc gacctccatc
      541 ccggcgccgc gcgcgggctc gacgatcgcc gcccgcagtt cgggttcggt catcgggccc
      601 accacaaccc gaaagccaca ttcgtgtcgc ggctggcgcc ccgaaagcca cattcgtgtt
      661 caccggcccg ggcagatcac tggtgtgcgc gaccggccgg cgctccccct cggcgcgccg
      721 gccggccggt ggtgcgccgg accctccccc tggcccggtc cggcgcggtg gcttccgcgc
      781 gcgtcccacc gcgggacgga agccgcggtg accccggctt tcggccctcc ccggccgccg
      841 gacgatcacc gcgatttgaa cactacgcgt gagcgccgcc accctgctaa gagttcacta
      901 agtagatatt tcacctgtcc gccaaaatct tccgctcgat ggacatggct ggtgacttac
      961 cgctattcgg ccactgcggc gagttccgca cccagttccg ccggccacgg cgcgcccgcc
     1021 tgccggaacc gttcttcggc gagttcgagc tgctcacggg cctgcacccg gtccccgcga
     1081 gcccgcagca cccgccccag cgcggcccgc gaaacgccct cctgcaagac gaatccacgg
     1141 ctggtggtca gctctatcgc ctgccgcgca tatcgttccg cctcgcccaa ttccccggtt
     1201 ccgaggtgca gctccgccag gttgttcagc gataccacag cgtaactgtc gtcccccagc
     1261 tcacggtcga tcgcgagcga gccgagctgg tgctcgatcg cctccgcgta ccggccggac
     1321 agcttctccg cctccgcgca gttgttcagc gcctgcccgc gcaacgccgg gtcgccggtc
     1381 cgctcggtct cctcgatcgc ctgccgcagc cggtcgatcg cctcgtcgta ccggccggag
     1441 ctgctcagcg ccgcgcccac gtggatgttc gccgacacca gcagccgctt gtccccgacc
     1501 tcggcggcca acgccagcgc gtgctcggcg tcggtcagcc cgccgccctc gatgccgaag
     1561 gtgagcgcga tggcgcaccg cgaaatgagc atccagcagt gcgcgagcgg gttcccggac
     1621 gccttcgccg cggccaggcc cagctcgacc aaccccgtcc actcgtcacg caacggccgc
     1681 accacccgat aggtgtggac cacgcgcgcg agctgccagg cgtcctcgta gcgcccggcc
     1741 cggtacgcgg cttcgcaggt ttcgagcagg ttcggccatt ccagcgcgaa ccagtcgagc
     1801 gcgtcgtcga agtcggcgaa ttcgggcagc tccgcttcgg gcagcgcggc ggcgaagtcg
     1861 agcgagtcca cgatgcgcag cagccgccgc cgcgcccggt cggccgcggc ctggtagtac
     1921 cgcaccgacc gccgcagcac cgcctcgcgt tcggtggcgg ccagctcggt ttccgcgagc
     1981 ccgcgcaggt acagccggac caggtcgtgt ggtgcgaaca cgtcccggcc gacctcggtg
     2041 agcaggtggt aggcggccag cgtgcgcagt cggcggcggg cctcggcgac cccggtgccc
     2101 ccgagcgcgg cggccaggtg cggcccgatc gacggcccgg tgaacgcgcc cagcaaccgg
     2161 aacaacgcgc cgaccggctc cggcagcccg cggtgcgaca cgtccagcgc ggcccgcacg
     2221 ctggtctccg cgacgtccac cccggccacg tcgagcgcgg ccagccgggt gcgctcgtcg
     2281 gccagctcag ccaccaggtc ctcgacggtc cactgtggac tcaccaccag ccgggcaccg
     2341 gcgatgcgca gcgcgagcgg caggtacgcg cacagctcgg ccagcttgcc gtagtccccc
     2401 ggcccggcca gttcctcgat caaccgcacc gcgtcggccg gtggcatgga ttccagcgcc
     2461 cggaggcgtg ccgcgctggc gaccacgagc ccgtcgagcc gcgcgcggct ggtcaccacg
     2521 gcgaccgagc ccggcgcggg cggcagcagc gggcggacct gctcggccga gcgcgcgtcg
     2581 tcgagcacca ccagcacggt ggcgtcggcc agcagcgagc ggtacaacgc gatgcgctcg
     2641 tgtggcttct ccggcagttc ggcgggccgc acgccgagcc cgagcaggaa ctgggtgacc
     2701 acctcacccg gcgccagcgg cgggtgctcc gggtcgaacc cgcgcagcgt cgcgaacaac
     2761 acgccgtccg ggaagtccgc cgctctccgg tgcgcccacg agatcgccag cgcgctcttg
     2821 ccgacgccgg gcgcgccggt gaccacgccg accatgctcg cgccctgccg ggcttcctcg
     2881 accaggccgt ccagccaggc cagctcgtcg gcgcgcccgg cgaagcgggg caccgcggcg
     2941 ggcagctgcg ccgggttgac ccgcaccagc cgcggtggcg gccccttcgg cgacgacggg
     3001 tcggcgaggt cgtcacgcag gatgcggtcg tgcagctccc gcagttcggc gccgggatcg
     3061 atgccgagcg tgccgaccac gtgccgggcc gcgcggcggt acaccgcgag cgcgtcggcg
     3121 cggcgtccgg cgtggtagag cgcacgcatc agctggccga tcgtgcgctc cgccaacggg
     3181 ttctcgcgca ccatggtggt cagctcggag atcagctcgg cgtgcctgcc gagttcgagg
     3241 tcggcgtcca cgcgggcgcc gtgcaccgcg agccgcaggt cctccagttc cggcgcgtgc
     3301 accgaatcgg ggacgccgga cagcaccggc cccttccaca acgcgagcgc ctcggcgagc
     3361 acggcggctc ggcgctcggc cccggccgcg gtggcgtact cgagcagcgc ccgcgcgcgg
     3421 tgcacgtcga tctgcgacgg gtcgacggtc agctggtagc cgggcgggcg ggtgtggatc
     3481 tgcgcgccgt cgccgctgtc gccctggatg gaccgcagca cgcggcggag gtgcgagacg
     3541 ttgccgtgca cgatggtgcg cgcggtggcc ggtggatcgt gcccccagag cgcgtcgatg
     3601 atgtcgtcca gtggaacaac tttgttggcg tgcaaggcca gcagcgccag cagcccccgc
     3661 acccccggcc cgccgaccgg caccggccgc gagcccgcca gcagctggac cgggccgagc
     3721 agctggaacc aggcctggtg ggaccccaaa tccccttcgc tcactacgcc tcccctgcgt
     3781 cgtggacgag caggagaaga cgttactcgc aaacccggca accggcgtgt ggagttgggc
     3841 atgcgcggcg ccggtggaac aacccccgga cacaacaccg ccctgcacca tcagtcccga
     3901 tctgatggtg cagggcggtg ttgccgactg ccacggccgg cgcgcgggcg gtctcacccc
     3961 gagcaaggag taaccgccgc gtcacttcga ccgtgacgga gacaacgtaa caggccacga
     4021 agggttagcg aagagccacc ccttcgcggc ctgataacga ttatcgccgg accggggtga
     4081 agtcgcgggc cgcgatgaac tccggccgtc gcttctccgc ggcgaacggt tccaccaccg
     4141 cgttgtccac gctgttgaac accaggaaga tgttcgagcg cgggtacggg gtgatgttgt
     4201 tgccggagcc gtgcatgatg ttcgagtcga accacagcgc cgagccggcg gcaccggtga
     4261 actgctcgat gccgtgctcc gcggcgagct tggcgatctc gtcctccgac ggcacgccga
     4321 tcttctgctc cttgagcgag gacttgtagt tgtcctccgg cgtccgcccg gcgcactgca
     4381 cgaacgtccg ctgcgaaccc ggcatcacca tcagcccgcc gttgaacggg tagttgtcgg
     4441 tgagcgcgat ggagcagctg accgccctcg gcaccggcat gccgtcctcg gcgtgccagg
     4501 tctcgaagtc ggagtgccag tagaaaccgt tgccgcggaa cccgggcatg tagttgaccc
     4561 ggctctgatg gacgtacacc tcggacccga ggatctgccg cgcccggtcg agcacgcgcg
     4621 gatcccggac cagctcggcg atcaggtcgc tgatccggtg cacctcgaag atcgagcgga
     4681 cctcgccgct gctcttctcg gtcaccactc gctcgtcggc cttcagttcc tcgtccccgg
     4741 acagccggac gagttcctgc cagtaggtct gcacctcggc cggggagagc aggccctcca
     4801 ccaccgagta gcccttggcg tcgtgcgaag ccaaggtggc cgcatcgatc ggaccgtccg
     4861 cctcaccgcc ccacacggtg gggtcgaggc ggtcgatcgc cccggcgtcg gccgccggga
     4921 gtcgggtcgg gtaggcgtcc ccgatccggg tgtccatcag agtcaaactg ctcgcctcct
     4981 cgtgatcgtg ctcttttcgc tcaggactct tcggtcacca gcgggtacac gccgttctcg
     5041 tcgtggacct cgcgtccggt gaccggcggg ttgaagacgc agaccgtctt catgtcggtc
     5101 ttcggcagca cctggtgctt gtcgtggtcg ttgagcaggt acagcgaacc gggcttgagc
     5161 tggtggcgct cgccggtggc cttgttgacc agctcgccct cgccctcgta gacgaacacg
     5221 gcctcgatgt ggttggcgta ccagaagtcg ttgaccgtgc ccgcgtagag cgtggtctcg
     5281 tgcaccgaga agccgacctt ctccttcgcc aggatgatgc gcttgctgcg ccagttcggg
     5341 gtcttgatgt cggcgtcggt gtcggtgatc tcgtcgaggg tgcggacgat caaagtcgtg
     5401 tctccttagc ttcctacttg gtcagcacgg cgcgaacgga ctcgccgatg atctccaggc
     5461 cctgctccag ctcggcgtcg ttgatggtca gcgcggggag cagcttcatc acttcaccgt
     5521 cgggcccgga ggtctccatc agcagaccac gatcgaaggc ctccgcgcac accgagccgg
     5581 ccagctcgcc gctggcgaac tcgatgccgc gggccagtcc acggcccttg gccaccaggt
     5641 tcgcctcggg gtgggcttgc acgatctcgt cgaacaccga gccgatgcgc tcgcccttcg
     5701 cgcgggtgga ctgctcgagc ttgtcgtcgc tccagtacac gcgcagcgct tcggtggcgg
     5761 tgacgaaggc cgggttgatg ccgcggaagg tgccgttgtg ctcgcccggc tcccacacgt
     5821 ccagctccgg cttgatcagc gtgagcgcca gcggcaggcc gtacccgccg atcgacttcg
     5881 acaggcagac gatgtccggc ttgatgccgg cgtcctcgaa gctgaagaac gggccggtgc
     5941 ggccgcagcc catctgcacg tcgtcgagga tgagcaggat gttgtgccgt tcgcacaggt
     6001 cggacaggcc cttgagccac tccagcctgg ccgcgttgat gccgccttcg ccctgcagcg
     6061 cctcgacgat caccgcggcg ggctcgttca ggccgctgcc ggagtcctcg agcagccgct
     6121 cgaagtacag gaagtcgggg taggcgccgt cgaagtactt gtcgtacggc atcggggtgg
     6181 cgtgcaccag cggcaccccg gcgccgccgc gcttcatcga gttgccggtg accgacagcg
     6241 cgcccagcgt catgccgtgg aaggcgttgg tgaagttgat gaccgactcc ttgccggtca
     6301 ccttgcgcgc cagcttgagc gcggcttcga cggcgttcgc gccaccgggg ccggggaaga
     6361 ccaccttgta gtccagctcg cgcggcccga ggatcttctc ctggagcgtc tccaggaagt
     6421 cgcgcttggc cacggtgaac atgtccagcg cgtgggtcac gccgtcgcgc gcgatgtagt
     6481 cgatcagcgc ctgcttgagc gccgggttgt tgtggccgta gttcagcgcg cccgcgccgg
     6541 cgaagaagtc gagatagccc ttgccgttct cgtcgtagag catgctgccc tgcgcgcggt
     6601 cgaagaccac cggccagccg cggctgtaac tccgcacctc ggattcgagt ttcgagaaga
     6661 tttccatgtt catcgccagt tccgtccgtc cctgtggagt gatcccgttg cggtgtgcga
     6721 attatctgtg cgtcaacggt ccgattcgat agagatcctc gggttcgtgc acaacgttgt
     6781 cttccgggaa atcgtcggaa ctgaacagtt cagcgctttc cagatcggct tcccagcgct
     6841 tcgcgaagga ctcgaagagc ttgatggacg cctggttgtc cggggtgatg gtggtctcca
     6901 gatagcggac gccttggccg accaagcggt cgaacaaggc gtccagcaaa gctccggcca
     6961 gcccttttcc gcgttgtgag gcgtcgacgg cgacctgcca gaccagcgcc gtctcgggat
     7021 cggacggctt gcggtagccg atcacgaagc cgaccgccgc gtcaccggat cgtgcgacca
     7081 ccgaggtgtc ggcaaaatcg cggcaccaca gcagataggc gtacggcgag ttcagatcga
     7141 gcttctggga atcgcgcgcg attcgccaga gttcggcgcc atccgccttg gtcggggatt
     7201 cgatcaagtg ctttccggac atgtcaaacg aacttaacag aatcgcaaag ccgactcagc
     7261 ccggacgaac tagtgcaact ggcctaccag cgcagacaca gaaaaccctg tgactatggc
     7321 aacagtaacg taggtttgcc gcaatcggtg gactacaatg ccgcagccga ccgccaccgc
     7381 ttcgtgacga agcggtttca cttcggacac ggtagtggcc acgctcacga gatgcgactc
     7441 aaagagcacg tttggcctaa gcatacgctg ggtactccgg tacttttccc agcgggagcc
     7501 acctattcgc cgacctggcc gtcggcgaac tcacgcagca cgtcgaggtg cccgacgtgc
     7561 ctcgcggtct cctgcaccag gtgggtgagc acccatcgaa cggtcttgtc ctcgcccggc
     7621 cgtcgcaccg ggtcatcggc cgacaacccg gccagggcgg cacgcgagat ccgccattcc
     7681 tcctggtagg ccgccaccag ctcggccggc gagtgctcct tgccgaggcg gaagaggaag
     7741 tcgtggtcgc cggtgtcgcc ccagagatcg ggcagctcgc tgccgccgcc gacgatggtc
     7801 agccaccacc gctcgaccgc ggtcaggtgc cggatcaccc cgagcgcgtt catctccggc
     7861 gacgccggga acggcgtgcc cgcggcctcg gcccggctca gcccggccac cttgttgacc
     7921 gccgtggccc gcaggaagtc gaggaagccc cagagcaggg tcagctcgtc gtcggcccgg
     7981 gtttccggcc acgcccgctc gaccggatcc gccatttcct tcgaactcga gttcgacacg
     8041 cggaaaaggt atcaggcggc gaagtgttcc agcagcagga cctgcgcctc acccacggcg
     8101 aagccgagca gcgcgccggt ggcgatcagg atccattcgt cctgctggaa ggcgggccgg
     8161 agcaggccct cgaactcggc gggcgacagc tgtttcatct tggtcaccag caggttccgg
     8221 atgtccatcg cgtcttcggc gtagtcctcg acgtaggtca tcgtctccgg cagccgcgcc
     8281 atgatcagct cggagatccg cagcttcatc gcctggtagc gcttgctgcc caccgccatc
     8341 accaccagcg gcctggccag gctggtgtgc ttggccagct cggcgtccac ctggcgctgg
     8401 accagcgcga gcaccttgtc cgacgcgggc ccgcgcagca ccgcctcgat cacgttgtgc
     8461 ggggtgatga tctccttggc gatcagcgcg ccgtaggcct cggacacctc ggcgcggcgc
     8521 ttgaggaaca ggccctgcca ctcgaacagc ccgaggtagc gcttcggctg ctgcggccgg
     8581 aagatcatct tcagcgccag ccagtcggtg aaccagccgg tgaacaggcc gaacagcggc
     8641 atgatcagcg gcgccttgaa caggatccag gcgcccatct ggatcacccc gatcagcccg
     8701 ccgaagacga tgccggagcg ggcgatgaac ttgaactcct tgtcgcccgc ctcctggaag
     8761 atccggttca gcagccgctt gtccttgacc aggctggtga ccaccatgtc cttgaggtcg
     8821 aagacgctgt ccacgtcctg cttgaccccg tccatgatcg aggcgaccat gcgcggcgcc
     8881 tccgcctgca cccgcgtgat caccattcgc tgcacgcgcg tcggcagcga ttcccacagc
     8941 ccgggctggt attcggcggc caccacccgc acgatctcct cgaccccggc cagcagcggc
     9001 ttctcgatct ccttggcgat gcgctccggg tcgagccggt tgagcacgtc acccggcttg
     9061 atcagctgct gggtcatcgt gtcgcaggcg atgccagcca tccgcgcggc ccgcttcggc
     9121 accaccccct gccagcccag gaatggtttg atgccaacga attccaccgg ctggaacatc
     9181 atccggatgg ccaccagctt ggtggtgtag ccgatcagcg cggcgatcag cgggatggag
     9241 cagtagagcg gccagttccg cgcgaagtcg tcgatgatgc cgctcatggc tcatgatcac
     9301 acgggggtcc acctgggaca atgatcagct tcgcgcgggg tcgcaggccg cccagaattc
     9361 cttgcccaac cgcgagatgt gcacggtccg gcgaacgaac ttcacccgtt tgaccgagtt
     9421 ctccgcggcc cggaccgcgt cgtcggtgag caggatttcg tactgggtct cgagttccgg
     9481 cacttcctcg tccggttcgg ccaggccgag accgatcagc cgggtgacgt aggcgggcac
     9541 ctggtcgagc agggtcaccc cggccgcctt gcccacggtg gacgcgttgc gcaacaccac
     9601 ccggcccgcg ccgccgagcc cgttgcgctc ggtgacgtcg atcagcggga acggcgcccc
     9661 gtcggacaac gcggagagaa tgcgtgcttc gtccggcgtg agctggcgca ggatgatcgc
     9721 gtacaggtat tcgcgggcgc gctcgcggcc gaatccgatg gatcggttca gcagctcggc
     9781 catcgccgcg cgcagcggct cgacgctctc cgaggacggc atcaccacca cggtgccgcg
     9841 gatctcgccg ttcaacggct ggacgtgccg cccgttgccg ccgacgttca tcgccgacgc
     9901 ggcgctcagc gcggccaggt acgggtcgtc gacctcttcg aggcggcgcc gcagctcggt
     9961 cagcgcgagc cgttccagcg agcgcaggcc gcgctcggcc gcgtccgcgc cgggcagccg
    10021 cttgccgagc gagtaacccg cgcgggcggc gatgctggcc agcttgcccg cccgctcggc
    10081 gaagttctcc ggagctccca cggcgccact cccctccttg acgcccggaa tactactttt
    10141 cagtaggttc gctgggccga acggacgtcc gttgcctcca tcacaccacg agccaggcct
    10201 tcgcggcggc caggccctcg agcgtgatct ggtggccgcc cgcgtgccgg tgctcggtga
    10261 cctcggccga ccgttcccgc aggaagtcca cgtactgctc ggtcgaggcc agcggcgcca
    10321 tcgggtcgcg ctcgccgttg gacaggaaaa cgcgggtgcg ccgcagctcg cgctccggcg
    10381 gctccggcac cgg
//
