LOCUS       NZ_CP015163            10801 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Amycolatopsis albispora strain WP1 chromosome, complete genome.
ACCESSION   NZ_CP015163
VERSION     NZ_CP015163.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis albispora
  ORGANISM  Amycolatopsis albispora
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9121443)
  AUTHORS   Wang,H., Chen,S. and Wu,Q.
  TITLE     Complete genome sequence and analysis of deep-sea sediment isolate,
            Amycolatopsis sp. WP1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9121443)
  AUTHORS   Wang,H., Chen,S. and Wu,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2016) Zhejiang University of Technology, College
            of Pharmaceutical Science, Chaowang Road No.18, Hangzhou 310014,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera assembler v. unspecified
            Genome Coverage                   :: 138.17X
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 04:57:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,537
            CDSs (total)                      :: 8,472
            Genes (coding)                    :: 8,394
            CDSs (with protein)               :: 8,394
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 26 of 78
            Pseudo Genes (incomplete)         :: 54 of 78
            Pseudo Genes (internal stop)      :: 6 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:20:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2985477
            Orig. end                         :: 2996278
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015163.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10801
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="17"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10801
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10801
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5801](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5801
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(864..879)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.97"
                     /tool="antismash"
     gene            1360..1677
                     /locus_tag="A4R43_RS13890"
                     /old_locus_tag="A4R43_13775"
     CDS             1360..1677
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457580.1"
                     /locus_tag="A4R43_RS13890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_13775"
                     /product="hypothetical protein"
                     /protein_id="WP_113692721.1"
                     /transl_table=11
                     /translation="MAAAKNSTGRSDDEPNPRFHEDLVGLDPADPEAQEFARHLDRIER
                     CGPAFTVEASLDGVAEFADSSRRAGGLRWWTAVAVVCLILIGVIVASWDIVLRTLAWLA
                     E"
     gene            1697..2119
                     /gene="msrB"
                     /locus_tag="A4R43_RS13895"
                     /old_locus_tag="A4R43_13780"
     CDS             1697..2119
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671916.1"
                     /locus_tag="A4R43_RS13895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_13780"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_113692722.1"
                     /transl_table=11
                     /translation="MKPVVGATPRVVKSEQEWREQLGPEEYAVLREAGTERPFTGEYTD
                     TKTTGVYECRACGAELFRSDTKFESHCGWPSFYDPADSDAVLLREDRTMGMVRIEVLCA
                     SCHSHLGHVFEGEGYDTPTDQRYCINSISLKLVPKP"
     gene            2191..3093
                     /locus_tag="A4R43_RS13900"
                     /old_locus_tag="A4R43_13785"
     CDS             2191..3093
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="A4R43_RS13900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_13785"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_113692723.1"
                     /transl_table=11
                     /translation="MADPWGLSGPVFLIIYACCLAVPTVVLASGLVRLRSGTAGAGQGP
                     LDAVQAAFLRGGPRRAAETVLAGLIERGLLRADSSGTLRHTGGAGANPLEQQVLASTSG
                     AGGRTPLTLVTGLGRSGAMQALRADLVAKGLLVDARRRRAVCFTALGLYGLVLLLGLFR
                     IAVGVAGQFPVGYLTLMVVAALAGLITTSTLLVRGGDQPTQEGRRTHTATFPGAPGKVA
                     AHGLSSYPDHKIRKALRYSMALPRRRRSNSSCGSYAGGAMVSGTGCASAGSGCSSSSSS
                     GGGSSCGGGGGCGGGGGGG"
     misc_feature    complement(3096..3111)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.17"
                     /tool="antismash"
     gene            3097..4017
                     /locus_tag="A4R43_RS13905"
                     /old_locus_tag="A4R43_13790"
     CDS             3097..4017
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="A4R43_RS13905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_13790"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_113692724.1"
                     /transl_table=11
                     /translation="MDETWGLTGSGFLGLYLALLVVPGLLGALVTLAVRQLRLHGSAPD
                     DSPLPTDYHVAYLARGPLRAVETAIAALVDRGSLRAESGGRVTSVGGEPEDDLERVIFE
                     TGYLGTTTNEAAVAALKSPELTEIRRDLELRRLIEPELPTHVIWLGVTVLYGLVTLFGL
                     VRWFAVVTTSTPIGFLSFLIFVAAGAAFAALAPARRRPRLLKTELGKQVLRASRQNLTG
                     VTREVALNGGLSAYPDPEVAPVLELKFNRSRRRYPGTSSAGVIAGTLLAGAFGHGVPGD
                     SGAGDGGGCGSGCGGGCGGGGGGGS"
     gene            4097..4999
                     /locus_tag="A4R43_RS13910"
                     /old_locus_tag="A4R43_13795"
     CDS             4097..4999
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="A4R43_RS13910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_13795"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_113692725.1"
                     /transl_table=11
                     /translation="MKDPWGISGPGFIVGYLCLLAAPLVIALVGRIVLAFTGKRAATAY
                     VPGNVYEVAFLAGGHDRVVETAVASLLERGVFRADSTGRVSAAGAVPSDPVEQAVYRVV
                     ARRGTTRMPDLRRELRSAAVLRGMAKHLTDAGLIRPIGGRVVLRRMVFSLYLLVLGIGV
                     VRLISGIALDRPVGVLIFLLIIAAVATFIASRVAKAAPMVRPTAQGSVQLASARKAQSQ
                     RHSTRSGGPHRSSAAVPILAGAAGAVALGGMMMYPDEELRTALAAPPAGGSSGGDGGGS
                     SCSGGSSGCGGGCGGGCGG"
     gene            5001..5801
                     /locus_tag="A4R43_RS13915"
                     /old_locus_tag="A4R43_13800"
     CDS             5001..5801
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645126.1"
                     /locus_tag="A4R43_RS13915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_13800"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_113697578.1"
                     /sec_met_domain="DUF692 (E-value: 3.8e-83, bitscore: 277.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRFGVGIGWRPELDLTVPRLPGVDWVEVVAENLQPGALPATLTA
                     LRDRGMPVLPHAVSLSLGGAEELDTRRVEHLAELTEALGAPVASDHVCFVRAGGLDAGH
                     LIPLPRTREALDVLVDNVKLAQSILGVPFALENIAALLEWPDPELSEARFLAELTDRTG
                     CLLIVDVANLYANARNLGSDPVSFLDEIPLDRLAYVHVAGGEEHHGIYHDTHAHAVPPP
                     VLDLLGELRSRVDPPGVLLERDDAYPTDAELAAELAAIRAVIEP"
     gene            6255..6602
                     /locus_tag="A4R43_RS13920"
                     /old_locus_tag="A4R43_13805"
     CDS             6255..6602
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004562080.1"
                     /locus_tag="A4R43_RS13920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_13805"
                     /product="hypothetical protein"
                     /protein_id="WP_113692726.1"
                     /transl_table=11
                     /translation="MSREHLAAQQADLLRALLADGTPPPGFDTDRVAVEVRALRAKRRR
                     VVAYLRPDLAQELDDRFTPLFDEYAAAHPREEGVRMRQDAENFANWLTAKGELKPPRWW
                     RRLPGRAIRAR"
     misc_feature    6573..6575
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6573..7907)
                     /locus_tag="A4R43_RS13925"
                     /old_locus_tag="A4R43_13810"
     CDS             complement(6573..7907)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1189: L-carnitine dehydratase/bile acid-inducible"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075645.1"
                     /locus_tag="A4R43_RS13925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_13810"
                     /product="CoA transferase"
                     /protein_id="WP_236809208.1"
                     /transl_table=11
                     /translation="MEEELAAVWRQVSGESLPPGAVAVGGEESVLPGPFRVAAAAAASV
                     GVATLAAGELLRVRGIEPPPVAVDLRHAVASFHSERHLRVDGQVPGELWAPLTGDYLAT
                     DGWVRLHCNYPRHVAAVCWGLGTAATREAVTEAVAKRRAREVQEAVIAAGGAAALLRTR
                     QDWLNHPQGEAVAGQPLVELRQTGDALPKRLFESDRPLGGVRVLELTHVIAGPVAGRVL
                     AAHGADVLHIGAAHLPRIDPLWMDTGLGKRSAFTDLTQDAGRARLRKLLARADVFVQSF
                     RPGALARWGFSPEELAYEFPGLITLDLSAYGWHGPWRNRRGFDSLVQLATGIAAHAGLD
                     HPRPLPAQALDHATGWLAAATVMTAVRRAATDGGGWRGQVALAHTAEWLESLGRREITD
                     TGYQVDDLLATLPSAYGELTYVPVPGANPQWTEGTRLPGSDRPSW"
     misc_feature    7896..7912
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.64"
                     /tool="antismash"
     gene            complement(7917..8561)
                     /locus_tag="A4R43_RS13930"
                     /old_locus_tag="A4R43_13815"
     CDS             complement(7917..8561)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A4R43_RS13930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A4R43_13815"
                     /product="hypothetical protein"
                     /protein_id="WP_113692727.1"
                     /transl_table=11
                     /translation="MSFPEINRKQKQLRHDVDDVYELLDDTNKRVDVIDKRIDGIDTRV
                     DAIDTKVASVLEVQHDHGAQLSRMQATLEAHTEALAHHETRFDTIDTRLNRIDTRLDGL
                     DTRLGEYDARFGRIETRLDGHDIEFENIKVRLDAQSLMLSGQAAKIDSQGAMLDRQGAM
                     LDSQAAKLDSQGAKIDSQGVKLDSHGAKLDSQGEQLRQIIELLSGQRPESN"
     misc_feature    complement(8542..8555)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
     gene            complement(8612..9304)
                     /gene="hemQ"
                     /locus_tag="A4R43_RS13935"
                     /old_locus_tag="A4R43_13820"
     CDS             complement(8612..9304)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="A4R43_RS13935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_13820"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_113692728.1"
                     /transl_table=11
                     /translation="MARLNYNELNDTIRYTAWSVFRAEPGRLPEDRGPAARETAEFLDG
                     LESKGVVVRGVYDVAGLRADADYLIWWHAEEIEQVQAAYTGFRRTPLGRASVPVWSQVA
                     LHRPAEFNRSHIPAFLAGEEPRKYVCVYPFVRSYEWYLLPDEERRKMLADHGKEARDYP
                     DVRANTVASFALGDYEWILAFEADELHRIVDLMRHLRGTEARLHVREEIPFYTGTRVPP
                     AELIQNLP"
     gene            complement(9292..10716)
                     /gene="hemG"
                     /locus_tag="A4R43_RS13940"
                     /old_locus_tag="A4R43_13825"
     CDS             complement(9292..10716)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422378.1"
                     /locus_tag="A4R43_RS13940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_13825"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_113692729.1"
                     /transl_table=11
                     /translation="MVTRIAVVGGGVSGFAAAYRLRRLLGAGAELTVFESTGVIGGKLR
                     TGTLAGRPFDLGAEAFLARRPEAVALARELGLDVVHPTGARATVRAGAQARQLPPGTVM
                     GVPASADAVAGVLSPDGLAAVAAEAALPPVVLPEGDVPLGRLLRSRFGDELVDRLVDPL
                     LGGVYAGGADGLGLRATMPGLAGALDRGAATLTEAAASLVPASPSGAPVFGTLSSGLGT
                     LIDRLAAASGAEVRTGTTVRELHRLPAGWRLVVGAAAPAHAPEESTVDFDAVLLAVPAP
                     AARRLLDGVAPAASSAFGQVELASMAVIGLALPPGTELPDASGVLVGASERRADGRPFA
                     VKAFTFSARKWAHHTGDDGVVLLRGSVGRFREPGALHADDDELVRLVLDDLAELTGVRA
                     EPVDTVVTRWGGGLPQYGAGHTELVASIDRAVAGLPGLEVAGATLRGVGIPACVATADA
                     AAARLAEFAKMDAWRG"
     misc_feature    10710..10724
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.18"
                     /tool="antismash"
ORIGIN
        1 agcgcgtgcc ggacaggcca ggccgctcgc cgcggcgctc ggcgagcgcg gggtcgccgc
       61 cgtcgcggtg acctgggtgg acaccagcgg catcacccgc gtcaaggcgg tcccggtgcc
      121 gcggctcgag cacgccgccg cctggggcat cggcgcgtcg ccggtgttcg acgccttcct
      181 ggtcgacgat tccgcggtga ccggccggtt cgccggcggc ccggcgggtg acctgcggct
      241 gcaccccgac ctggaccggc tgaccgtgct ggccgcacaa ccgggctggg cctgggcgcc
      301 cgccgaccgc taccagcagg acggcacacc acacccgggc gattcgcggc tcctcgcgcg
      361 ccaggccgtc gcccggctcg ccgaactcgg cctgaccgcg cggctggcct tcgaggtcga
      421 atggtgcgtg tccctcggtg acggtgacga gttcgtcccg gcgagtccgg gcccggccta
      481 cggcatgtcc aggatcgccg acctgtccgg ttacctgcac gaactgctgg aggcgctgag
      541 cgcgcagggc gtggccgtcg agcagttgca ccccgaatac gccgccggtc agttcgaggt
      601 gtcggtggcg gcaacggacc cggtgggcgc ggcggacctg ctggtactgg tgcgcgagac
      661 cattcgcgcg gtcggccggc gacacggcct gcgcaccacc ttcgcgccga aggtggtggc
      721 gggcggggtc ggcaacggcg ggcacgtgca cctgagcctg tggcgtgacg gcaccaacct
      781 gatggccgac ggcgacggct ggcacgggct caccgagctg gccgaggcct tcgcgggcgg
      841 catcctggac cggctgcccg gcctgctcgc catcggcgcg ccgtcggtgg ccagctacct
      901 gcggctggtg ccgtcgcact gggccggtcc gttccgctgc tggggctggg agaaccgcga
      961 agccgcgctg cgcctggttt ccggggagtc cggccacggc cagcgcgcgg cgaacctgga
     1021 ggtcaaggcg ttcgacgggg ccgcgaaccc gtacctggtg gtggccgcgc tgctcgccgc
     1081 gggctccacc ggcatcgccg agcaggcggc gctgcccgag ccgatccagg tcgacccggc
     1141 caccctgccc gaggacaccg tgcaacgcct gccggacaac ctcagcgcgg cggtcaccgc
     1201 cttcgaagcg gacccggcgc tgcgcaaggc gctcggcgag gaactggtgg acaccgtgat
     1261 caccgtgcgt cgcggcgagg tcgccttgtt cggcggcctc tccgccgaag aagtcgtggc
     1321 gcgcacccga tggcgctact gacgcgatac cgtgaagcca tggccgctgc caagaacagc
     1381 accggcaggt cggacgacga gccgaacccc cggttccacg aagacctcgt cgggctggac
     1441 ccggcggatc cggaagccca ggagttcgcc cgccatctcg accggatcga gcgctgcggg
     1501 cccgccttca ccgtggaggc ctcgctggac ggggtggccg agttcgccga ctccagccgc
     1561 cgcgccggtg gtttgcgctg gtggacggcg gtggccgtgg tgtgcctcat cctgatcggc
     1621 gtgatcgtcg cctcgtggga catcgtgctg cggacgctgg cctggctcgc cgagtaacgt
     1681 gggtggtgtg aaaggtatga agcccgttgt cggcgcgact ccccgcgtgg tgaagtccga
     1741 gcaggagtgg cgggagcagc tcggccccga ggagtacgcg gtgctgcgtg aggcaggcac
     1801 cgaacgcccg ttcaccggcg agtacaccga caccaagacc accggcgtct acgagtgccg
     1861 cgcctgcggc gccgagttgt tccgcagcga caccaagttc gaaagccact gcggctggcc
     1921 gtcgttctac gaccccgccg attccgacgc ggtgctgctg cgtgaggacc gcaccatggg
     1981 catggtccgc atcgaggtgc tgtgcgcgtc gtgccacagc cacctcgggc acgtgttcga
     2041 gggcgagggc tacgacacgc cgaccgacca gcggtactgc atcaactcga tctcgctgaa
     2101 actggtgccc aagccgtaac ccgttccggg gacagccgcc tcccccgccc gataccgtga
     2161 cgcgcacgct tcgggtggga ggggcaccga atggctgatc cgtgggggct gtccggcccg
     2221 gttttcctga tcatctacgc ctgctgcctg gccgtaccga ccgtggtgct ggcctccggg
     2281 ctggtgcggc tccgctcggg caccgccggc gcggggcagg gcccgctcga cgcggtccag
     2341 gccgcgttcc tgcgcggcgg cccgcgacgc gcggccgaga cggtgctggc cgggctgatc
     2401 gagcgcggcc tgctgcgggc ggattcctcg ggcaccctgc gccacaccgg cggcgccggc
     2461 gcgaacccgc tggaacagca ggtgctggcg agcacgagcg gcgcgggcgg tcgcacgcca
     2521 ctgacgctgg tcaccgggct cggccgctcc ggtgccatgc aggcgctgcg ggccgacctg
     2581 gtcgccaagg gactgctggt cgacgcccgg cggcggcgtg cggtgtgctt caccgcgctc
     2641 gggctgtacg gcctcgtgct gctgctcggc ctgttccgga tcgcggtcgg agtcgccggg
     2701 cagttcccgg tcggctacct gacgctgatg gtggtcgccg cgctggccgg cctgatcacc
     2761 acgtcgacgc tgctggtgcg cggcggtgac cagccgacgc aggaaggcag gcggacgcac
     2821 acggccacct tccccggagc gccgggaaag gtggccgctc acggcctttc gtcgtacccg
     2881 gaccacaaga tccgcaaggc actgcggtac agcatggccc tgccccggcg gcggcggtcg
     2941 aattcttcgt gcggaagcta tgccggtggt gccatggtga gtggtacggg ttgcgcgtcg
     3001 gccggcagcg gctgctcgtc gtcgtcctcg tcgggtggtg gcagcagttg cggaggcggc
     3061 ggcggttgcg gtggtggtgg cggaggaggc tgagcgatgg acgagacctg gggcctgacc
     3121 ggatccggtt tcctcgggct gtacctggcc ctcttggtgg ttccggggct gctcggcgcg
     3181 ctggtcacgc tggccgtccg gcagctgcgg ctgcacggct ccgcaccgga cgattcgccg
     3241 ttgccgaccg actaccacgt cgcctacctg gcccgcggtc cgctgcgtgc ggtcgaaacg
     3301 gccatcgccg ccctggtcga ccgcggcagc ctgcgggcgg aaagcggcgg gcgcgtcacc
     3361 tcggtcggcg gtgagccgga ggacgacctc gagcgcgtca tcttcgagac cggttatctc
     3421 ggcacgacca cgaacgaagc cgcggtcgcc gcgctgaagt caccggagct gaccgagatc
     3481 cggcgtgatc tcgaactccg ccggctgatc gagccggagt tgcccacgca cgtgatctgg
     3541 ctcggggtca ccgtgctcta cggcctggtc acgctgttcg gcctggtgcg ctggttcgcg
     3601 gtggtcacca cgagcacacc gatcggtttc ctcagcttcc tgatcttcgt cgcggccggt
     3661 gccgccttcg ccgcgctggc cccggcccgc cggcggccgc ggttgctcaa aaccgagctg
     3721 ggcaagcagg tgctgcgcgc gtcacggcag aacctgaccg gcgtgacgcg tgaggtcgcg
     3781 ctgaacggcg gcctgtcagc ctatccggat cccgaggtcg cgccggtgct ggagctgaag
     3841 ttcaaccgct cgcgtcgacg ctacccgggg accagcagcg ccggggtgat cgccggaacg
     3901 ctgctcgcgg gcgcgttcgg gcacggggtg cccggcgaca gcggcgccgg ggacggcggt
     3961 ggctgcggtt ccggctgcgg tggcggttgt ggtggtggcg gaggcggcgg cagctgatgc
     4021 cggtggaatt acggccagaa ctgtcggtgc actgacctag gctccggaca ggttcgattc
     4081 ggtgggaggc cgagtgatga aagacccatg gggcatctcg ggtccggggt tcatcgtggg
     4141 gtacctgtgc ctgctggccg caccactggt gatcgcgctc gtcggccgca tcgtgctcgc
     4201 tttcaccggg aagcgggcgg ccaccgcgta cgtgcccggc aacgtgtacg aggtggcgtt
     4261 cctggccggt gggcacgacc gggtcgtcga gaccgccgtg gcgtcgctgc tcgagcgcgg
     4321 ggtgttccgc gcggacagca cggggcgggt gagcgccgcc ggtgcggtgc cgtccgatcc
     4381 ggtggagcag gcggtgtacc gggtggtcgc gcgccgcggc accacgcgga tgcccgacct
     4441 gcgccgcgaa ctgcgctcgg cggcggtgct tcgcggcatg gccaagcatc tcaccgacgc
     4501 cgggctgatc cggcccatcg gcgggcgcgt ggtcctgcgg cggatggtgt tctcgctgta
     4561 cctgctggtg ctgggcatcg gcgtggtgcg gctgatcagc ggtatcgcgc tggaccggcc
     4621 ggtgggtgtg ctgatcttcc tgctgatcat cgccgcggtg gccacgttca tcgcctcacg
     4681 ggtggccaag gcggcgccca tggtgcggcc gaccgcgcag ggctcggtgc agctggcgag
     4741 cgcgcggaag gcgcagtcgc agcggcattc cacgcggtcg ggcggcccgc accggtcgtc
     4801 cgcggcggtg ccgatcctcg ccggtgccgc gggcgcggtc gcgctgggcg gcatgatgat
     4861 gtatccggac gaggaactgc ggacggcgct ggccgctcct cccgcgggcg gttcgtccgg
     4921 tggggacggt ggcggctcgt cctgcagcgg tggttcgtcc gggtgcggtg gtggctgcgg
     4981 cgggggttgt ggtggctgag ctgccccggt tcggcgtcgg gatcggctgg cggccggagc
     5041 tggacctgac ggtgccgcgc ctgcccgggg tcgactgggt ggaggtggtc gcggagaacc
     5101 tccagcccgg cgcgctcccg gcgacgctga ccgcgctgcg cgatcgcggc atgccggtgc
     5161 tgccgcacgc ggtgtcgctt tcgctgggtg gcgccgagga actggacacc cggcgggtcg
     5221 agcacctggc cgagctgacc gaggcgctgg gggcgccggt ggcgagcgac cacgtgtgct
     5281 tcgtgcgcgc gggcgggctg gacgcgggtc acctgatccc gttgccgcgt acgcgcgagg
     5341 cgctggacgt gctggtggac aacgtgaagc tggcgcagtc gatcctcggg gtgccgttcg
     5401 cgctggagaa catcgcggcg ctgctggagt ggcccgatcc ggagctcagc gaggcgcggt
     5461 tcctggccga gctgaccgat cgcaccggct gcctgctgat cgtggacgtg gcgaacctgt
     5521 acgccaacgc ccggaacctg ggcagcgacc ccgtgtcctt tttggacgaa atccccctgg
     5581 accggctcgc ctacgtacac gtggccggcg gcgaagaaca ccacggcatc taccacgaca
     5641 cacacgccca cgccgtgcca ccaccggtac tggacctact cggcgagcta cggtccagag
     5701 tggacccacc cggcgtcctc ctagaacgcg atgacgccta ccccaccgat gccgaactgg
     5761 ccgcggaact ggccgccatc cgcgccgtaa tcgagccatg agccggaaac agcggtccgc
     5821 ccgctatacg caccacgcac cacgcacccg acggtaccca ccccaccagc cgcaacccac
     5881 acagcggtca agccatgagc cgggaccagc gggccacccg ctacgcgcac cacacgccga
     5941 cgccaccccg ccaaccggct tcaacgcgcg ccaggcaaac gtcaagccac aatcgcaagc
     6001 ggcccaccgg catgccactg aaacacaaca aaacccacgc caccaacgcc gacctaaccg
     6061 caacccacac ggcgatcagc ccatgaaccg agcactaacc gcaatccgca cggtggccga
     6121 gccatgagcc agcaacagcg ggcagcccac tacgcgcacc acgcgcccac ggcaccccac
     6181 cccacccacc gcaatccaca cggcgatcag cccatgaacc gagcactaac cgcaatccgc
     6241 acggtggtcg agccatgagc cgcgaacacc tggcggcgca gcaagcggac ctactgcgtg
     6301 cactactcgc cgacggcact cccccacccg gcttcgacac cgaccgggtg gccgtcgagg
     6361 tgcgggcgct gcgcgccaag cgccgccggg tcgtcgccta cctgcggccc gacctggcgc
     6421 aggagctgga cgacaggttc acgcccctgt tcgacgagta cgcggccgcg cacccccgtg
     6481 aagaaggcgt ccggatgcgg caggacgcgg aaaacttcgc gaactggctc accgcgaagg
     6541 gcgagctgaa gccaccgcgc tggtggcggc ggttaccagg acgggcgatc cgagcccggt
     6601 agccgggtac cttccgtcca ctgtggattc gcaccgggca ccgggacgta ggtcagttcg
     6661 ccataggcgc tgggcagcgt cgccagcagg tcgtccacct ggtagccggt gtcggtgatt
     6721 tcgcggcgcc ccagggattc cagccattcc gcggtgtgcg cgagcgccac ctgcccacgc
     6781 cagccgccgc catcggtggc ggcgcggcgc accgcggtca tcaccgtcgc cgcggcgagc
     6841 cagccggtcg cgtggtcgag cgcctgcgcg ggcagcgggc ggggatgatc aagcccggcg
     6901 tgcgcggcga tgccggtggc cagctgcacc aggctgtcga agccgcgccg gttgcgccac
     6961 gggccgtgcc agccgtacgc ggacaggtcg agcgtgatca ggccagggaa ctcgtaggcc
     7021 agttcctccg gcgagaagcc ccagcgggcc agcgcgccgg gacggaacga ctgcacgaac
     7081 acgtccgccc tggccagcag cttgcgcagc cgcgcacgac cggcgtcctg cgtgagatcg
     7141 gtgaaggcag aacgcttccc gagcccggtg tccatccaca gtggatcgat ccgcggcagg
     7201 tgggcggcgc cgatgtgcag cacgtcggcg ccgtgcgcgg cgagcactcg ccccgccacc
     7261 ggcccggcga tgacgtgggt cagttcgagc acgcggaccc cgccgagcgg gcggtccgac
     7321 tcgaacagcc gcttcggcag cgcgtcgccg gtctggcgca gctcgaccag cggctgtccc
     7381 gcgacggctt cgccctgcgg gtggttcagc cagtcctggc gggtgcgcag caacgcggcc
     7441 gccccaccgg ccgcgatcac cgcctcctgc acctcgcggg cgcggcgctt ggcgaccgcc
     7501 tcggtgaccg cctcgcgggt agcggcggtg ccgagccccc agcagacggc ggcgacgtgg
     7561 cgggggtagt tgcagtgcag ccggacccac ccgtcggtgg cgaggtagtc gccggtcaac
     7621 ggcgcccaca gctcgccggg gacctggccg tccacccgga ggtggcgctc actgtggaac
     7681 gaagccaccg catgccgcag gtcaaccgcg accggcggcg gctcgatccc ccgcacccga
     7741 agcaactcac ccgcggcgag cgtcgcgacc ccgacactgg ccgccgcggc ggccgccacc
     7801 cggaacggcc cgggcagcac ggactcctcg cccccgaccg ccaccgcccc cggcggcagg
     7861 ctttccccgc tgacctgccg ccacaccgcg gcgagttctt cttcgatcac gttccctcag
     7921 ttgctctcgg gccgctgccc gctcaacagc tcgatgatct gcctcaactg ctcgccctgg
     7981 ctgtcgagct tggctccgtg actgtcgagc ttgacccctt ggctatcgat cttggcacct
     8041 tggctgtcga gcttggccgc ctggctgtcg agcatggcgc cttgcctatc gagcatggcc
     8101 ccttgactgt cgatcttggc cgcctgaccg ctgagcatca gcgactgggc gtccaggcga
     8161 accttgatgt tctcgaactc gatgtcatgc ccgtccagcc gggtttcgat ccggccgaac
     8221 cgagcgtcgt actcgccgag tcgggtatcc aagccatcca gccgggtgtc gattcggttc
     8281 aggcgggtgt cgatggtgtc gaaccgggtc tcgtggtggg cgagcgcctc ggtgtgtgct
     8341 tccagcgtgg cctgcatccg gctcagctga gcaccgtggt catgctgcac ttcgaggacc
     8401 gacgccacct tcgtgtcgat ggcgtcgacc ctcgtgtcga tgccatcgat ccgcttgtcg
     8461 atgacgtcga ctctcttgtt cgtgtcgtcg aggagttcgt acacgtcgtc gacgtcgtgg
     8521 cgcagttgct tctgcttccg gttgatctcg gggaaagaca tgtgggccac cttaccgccg
     8581 tcgttgccaa gtggcctatg ccaccccgtc gctacggcag gttctggatg agctccgccg
     8641 gcggcacccg cgtgccggtg tagaagggga tctcctcccg cacgtgcagc cgcgcctccg
     8701 tgccgcgcag gtgccgcatc aggtcgacga tccggtgcag ctcgtccgcc tcgaaggcca
     8761 ggatccattc gtagtcaccc agcgcgaacg aagcgaccgt gttcgctcgc acgtccgggt
     8821 agtcccgcgc ctccttgccg tggtcggcga gcatcttgcg acgctcctcg tccggcagca
     8881 ggtaccactc gtacgaccgc acgaacgggt acacgcacac gtacttccgc ggctcctccc
     8941 ccgccaggaa cgccgggatg tggctgcggt tgaactccgc gggccggtgc agcgcgacct
     9001 ggctccacac cggcaccgaa gcccgcccga gcggggtccg ccggaacccg gtgtaggcgg
     9061 cctgcacctg ctcgatctcc tcggcgtgcc accagatcag gtagtccgcg tcggcgcgga
     9121 ggccggcgac gtcgtacacc ccgcgcacca ccacgccctt gctctcgaga ccgtcgagga
     9181 actcggcggt ctcgcgcgcg gcggggccgc ggtcctcggg cagccggccc ggttcggccc
     9241 ggaacaccga ccaggcggtg tagcgaatgg tgtcgttgag ctcgttgtag ttcagccgcg
     9301 ccatgcgtcc atcttcgcaa actccgccag cctcgcggcg gcggcgtccg cggtggcgac
     9361 gcaggcggga atgccgacac cacgcagcgt ggcccccgcc acttccaggc cgggcagccc
     9421 ggccacggca cggtcgatcg acgcgaccag ctcggtgtgc ccggcgccgt actgcggcag
     9481 cccgccaccc cagcgggtga ccacggtgtc caccggttcg gcccgcaccc cggtcagttc
     9541 ggcgaggtcg tcgagcacca gccgcaccag ttcgtcgtcg tcggcgtgca gcgcgcccgg
     9601 ctcgcggaac cggccgaccg acccgcgcag cagcaccacg ccgtcgtcgc cggtgtgatg
     9661 cgcccacttc ctggccgaga aggtgaacgc cttgaccgcg aacggcctgc cgtccgcgcg
     9721 ccgctccgac gccccgacca gcactcccga cgcgtccggc agctcggtgc ccggcggcag
     9781 cgccagcccg atcaccgcca tcgacgccag ttcgacctgc ccgaacgccg acgacgccgc
     9841 gggcgcgacg ccgtcgagca gccgccgggc ggccggggcc ggcaccgcca gcagcaccgc
     9901 gtcgaagtcc acagtggact cttccggcgc gtgggcgggg gcggcggcac cgacgaccag
     9961 gcgccagccg gcgggcaggc gatgcagttc acgaaccgtc gtcccggtgc ggacctcggc
    10021 gcccgacgcc gcggcgagcc ggtcgatcag ggtgccgagt ccggagctga gcgtgccgaa
    10081 caccggcgcg ccgctcggcg atgcgggcac cagcgacgcg gcggcttcgg tcagtgtggc
    10141 cgctccccgg tcgagcgcac cggccaggcc cggcatggtc gcgcgcagtc cgagcccgtc
    10201 cgctcccccg gcgtagaccc cgccgagcag cgggtcgacc aggcggtcca ccaactcgtc
    10261 accgaagcgc gaccgcagca gcctgcccag gggcacgtca ccctcgggca acacgaccgg
    10321 cggcaacgcg gcttcggcgg ccaccgccgc cagcccgtcc ggagacagca ccccggccac
    10381 cgcgtcggcg ctcgcgggca cgcccatcac cgtgcccggc ggcagctgcc tcgcctgcgc
    10441 gcccgcgcgc acggtcgccc gtgccccggt cgggtggacc acgtcgagcc ccagttcccg
    10501 cgccagcgcc accgcttcgg gacggcgggc gagaaaggct tcggcgccga ggtcgaacgg
    10561 ccgtcccgcc agcgtgccgg tgcgcagctt gccgccgatc accccggtcg actcgaacac
    10621 ggtcagctcg gccccggcac cgagcaggcg gcgcagccgg taggccgcag cgaacccgga
    10681 gacgccaccg ccgaccacgg cgatcctggt gaccatcggt tacagcgagt gcacgagttc
    10741 gaccacccgg gtgatcactt cggggtcggt gtcgggcagc acgccgtgtc ccaggttgaa
    10801 g
//
