LOCUS       NZ_CP015163            12776 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Amycolatopsis albispora strain WP1 chromosome, complete genome.
ACCESSION   NZ_CP015163
VERSION     NZ_CP015163.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis albispora
  ORGANISM  Amycolatopsis albispora
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9121443)
  AUTHORS   Wang,H., Chen,S. and Wu,Q.
  TITLE     Complete genome sequence and analysis of deep-sea sediment isolate,
            Amycolatopsis sp. WP1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9121443)
  AUTHORS   Wang,H., Chen,S. and Wu,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2016) Zhejiang University of Technology, College
            of Pharmaceutical Science, Chaowang Road No.18, Hangzhou 310014,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera assembler v. unspecified
            Genome Coverage                   :: 138.17X
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 04:57:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,537
            CDSs (total)                      :: 8,472
            Genes (coding)                    :: 8,394
            CDSs (with protein)               :: 8,394
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 26 of 78
            Pseudo Genes (incomplete)         :: 54 of 78
            Pseudo Genes (internal stop)      :: 6 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:20:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1476143
            Orig. end                         :: 1488919
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015163.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12776
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="8"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12776
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12776
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7776](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7776
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            288..2210
                     /locus_tag="A4R43_RS07140"
                     /old_locus_tag="A4R43_07055"
     CDS             288..2210
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1226: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422207.1"
                     /locus_tag="A4R43_RS07140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_07055"
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="WP_113691592.1"
                     /transl_table=11
                     /translation="MTYTFDPLVPRPIDLPTTVDGPLADLDEAKIFSGPADPADRPAWR
                     ERLREWREDARARHAYTGTAYERPGAAWAARCYTVAQVWLWDELLYSFEEGRFTPARFL
                     ADARKRFGGLDAVVLWHAYPVIGLDDRNQWDYYRDVPGLTELIGALHVAGLHVFVDYNP
                     WDVGTRRGRDDLTELAELVADFGADGVFLDTLKKAEPEFVERLEQARPGIVLEGESKLA
                     VERIEDHACSWAQFFADSPVPGVLRAHWYERRHMQHHIRRWHRDHSEELQSAWLNGVGV
                     MVWEVVFGVWVGWSARDAATVRRMTTVQRAVSSLLLDGEWTPLAELAPEAEAAGIYASR
                     WELDGVTLWTVVNRGDQDYSGPVVAADSLFPGAVPARGIGAFVRVEPGTPEAEWLPGLR
                     ETLRGLPHDPDASFPHRLAVRVRPQRSTGTPEPDAVVVPAGPYVLTVRYRARETGMYQG
                     APYVDEWKPLPPRLHDARTLQRDGELANDVAVALTEVTNEQFAAFLAATGYRPAQPHRF
                     LAHWGNGESPGAPVTYVDLDDARAYCAWRGGRLPTEDEWQLAGDRLHRGEPAVWNWTES
                     EHSDGRTRFVLLKGGSDYRADGADWYVEGGRHAPDHSVKLLLPGLGQARSATVGFRCAW
                     DITAS"
     gene            complement(2197..3060)
                     /locus_tag="A4R43_RS07145"
                     /old_locus_tag="A4R43_07060"
     CDS             complement(2197..3060)
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672921.1"
                     /locus_tag="A4R43_RS07145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_07060"
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="WP_113691593.1"
                     /transl_table=11
                     /translation="MIKNVTVLGLGPMGRAMARTFLRDGYRVTVWNRTASKADALVAEG
                     AHRPATVREAVAASELVVLSLTDYDAMYTLLEPATDTLPGRVLVNLSSDTPEQARAAAK
                     WAAGHGAAHLTGGVQASPPQIGQPGAYTFYSGPREVFDAHREALSVLTETDYRGEDPGL
                     AAAYYQLTMDLFWTTTLGWLHSLALANAHGISADDFLPHAASLLGGLPEFLTFYSPRID
                     AGEYAGDAERLTMAAASVDHVVRTAEDAGVDTTLPEALRAAVRRGIAAGHGENSATSLV
                     EVFRKP"
     misc_feature    3147..3161
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.27"
                     /tool="antismash"
     misc_feature    complement(3177..3191)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.27"
                     /tool="antismash"
     gene            complement(3275..4252)
                     /locus_tag="A4R43_RS07150"
                     /old_locus_tag="A4R43_07065"
     CDS             complement(3275..4252)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1251: luciferase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673185.1"
                     /locus_tag="A4R43_RS07150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_07065"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_113697398.1"
                     /transl_table=11
                     /translation="MRLSVLDTSPIVQGATPSEALHNTVDLAKLADSLGFHRYWVPEHH
                     GMRGVASSAPAVVVGHLANATRRLRVGAGGVLLPNHAPIVVAEQFGTLAAFHPGRIDLG
                     IGRAPGGAKPAVAAVRPDRTVADFERQLAELRSFLHPAEDAPVKAIPVLGGNVPDLWLL
                     GSSAASAELAGRLGLAYAFAHHLNPGEAARSADAYREHFRPSEHAESPALLVSLSVIAA
                     ETDERAEWLAGSTRLKVLSRIRGTRIQLPSPEDAAAYPYTADDRAEIAIRSRSVLSGGP
                     ETVAAGLREVREATGTGEFMVTTPVYHHADRRRSYELLAALDHI"
     gene            4367..4939
                     /locus_tag="A4R43_RS07155"
                     /old_locus_tag="A4R43_07070"
     CDS             4367..4939
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559316.1"
                     /locus_tag="A4R43_RS07155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_07070"
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_113691594.1"
                     /transl_table=11
                     /translation="METSDQSVRDLLAANIKRARADRGLSLSELSRRSSIGKATLSQLE
                     SGAGNPTIETVFSLSRALELPISDLLEGGRGEMTVVRGDEQEVLAGEAVELRLLRRIED
                     GGLIVEVYDQRVRGTARQRSLGHVGTEHTIVQAGKLRVEVGDSAAELGPGDSVAFDASI
                     PHSYQAIGGEVRSVLLLQYRGTGPHVC"
     gene            5001..6152
                     /locus_tag="A4R43_RS07160"
                     /old_locus_tag="A4R43_07075"
     CDS             5001..6152
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225094.1"
                     /locus_tag="A4R43_RS07160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_07075"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_113691595.1"
                     /sec_met_domain="DAO (E-value: 5.2e-59, bitscore: 199.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYGTRAVVIGAGIVGAACARELSAEGFRVTVVDRGHPGGATTAHG
                     EGNVLVSDKGPGPELELARLSRRLWPEILDGLPGTDAVEWDAKGGIVVATTEAGARALT
                     GFADGQRNAGILAEPLDAAALAEAEPRLTREVTAAIHYPEDAQVQPVAAATALLAASGA
                     TLRTGCNVTGPLTDSGCLNGIRTTAGDLPADLVVNAAGPWAGEVAGVLGAPIDVRPRRG
                     EVLVTTPMPPTVFHKVYDADYVGAVVSDAAGLRTSAVVESTKAGTILLGSSRRQVGFDG
                     RLHIPALSAIAAKALRLFPGLAGVPVMRAYGGFRPYVPDHLPVIGADPRLPGLWHATGH
                     EGAGIGLSAGTGRVLRDLVAGRKPEIEVGPFRVDRPAVLGGAR"
     gene            6149..6418
                     /locus_tag="A4R43_RS07165"
                     /old_locus_tag="A4R43_07080"
     CDS             6149..6418
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225095.1"
                     /locus_tag="A4R43_RS07165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_07080"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_113691596.1"
                     /sec_met_domain="Fer2_4 (E-value: 2.8e-23, bitscore: 80.7,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPRPIRLTIDGEPVSGMDGQTIAGVLLASGRKAWRTDPAGQPRG
                     VFCGIGVCFDCVVTVNALRDVRACRRRAREGDDVRTQSRVDGER"
     gene            6415..7776
                     /locus_tag="A4R43_RS07170"
                     /old_locus_tag="A4R43_07085"
     CDS             6415..7776
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225096.1"
                     /locus_tag="A4R43_RS07170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_07085"
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="WP_113691597.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.4e-25, bitscore:
                     89.1, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 2.7e-07, bitscore:
                     29.8, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKHVVVIGAGPAGMAAAAAALRAGAVVTVLDSAEEPGGQYHRRPL
                     GARYRAAKAFDRVLGHPRCTWRADTSVWALERRDGRAPLVHALRGVADGADREAVCLDP
                     DALVLATGAHDRVLPFPGWTLPGVFTAGAAQALAKGERVAVGQHVVVSGTGPFLLPVAA
                     SLLQVGARVEAVLEANQPRTILRGWAQDLSELVPHTGKAARLARYAAGQLRHRVPYRMG
                     QAVVEARGDGRVEEVVVARLRADWSIVPGTERTLTADAVCVGHGFSAQLELPVAAGCEL
                     AGDTVRVDGDQRTTQPGVFAAGEITGIAGQPSAVDSGTVAGWLAAGGAPAEIAGALRRR
                     DRGRAFGRRLAEAHPIGDGWAGWLRPDTVICRCEAVDHACLTRALADPVTAGARSVKLS
                     TRAGLGPCQGRICGPAVAALTTAAGAEAAGAHHRPIAQPIRLGELANQRTEENR"
     gene            7773..8690
                     /locus_tag="A4R43_RS07175"
                     /old_locus_tag="A4R43_07090"
     CDS             7773..8690
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658768.1"
                     /locus_tag="A4R43_RS07175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_07090"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_113691598.1"
                     /transl_table=11
                     /translation="MSTKADLGGVVVATALPYREDAQAPAGLAVDYDRYAEHCRWLIDN
                     GCHGIGPNGSLGEYSSLTDEERRRVAQTAIEAVGNDGKVVVGVHGVGSHQAKHWAELAA
                     EDGAHGVLCLPPTMYRANRGEVVAHFEAVASVGLPVMVYNNPHDTKVDLTPDLLGELAG
                     IEHITAVKEFSGDVRRVLEIKEHAPDLAVISGADDVALEMLLMGSTGWFAGFPNVFPAE
                     SVELYELAVRGELTRARARYEPLVAAFRWDSRTEFVQAIKLGMDLAGRYGGPCRPPRGP
                     LVPAHDERVRADLARAMDALARRS"
     gene            8690..9691
                     /locus_tag="A4R43_RS07180"
                     /old_locus_tag="A4R43_07095"
     CDS             8690..9691
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646747.1"
                     /locus_tag="A4R43_RS07180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_07095"
                     /product="proline racemase family protein"
                     /protein_id="WP_113691599.1"
                     /transl_table=11
                     /translation="MRSIRTLTAVDSHTEGMPTRVITGGVAPIPGDTMAARRRHFIDHL
                     DDLRRFLVDEPRGHPAMSGAILQPPARPDADWGVLYVEVSGVLPMCGHGTIGVATVLVE
                     TGMVPVTEPETVVRLDTPAGLVHARVEVRDGVAQRVTLRNVDSFAVELDAVADVPGIGE
                     VRYDLAYGGNFYAIVDLASIGLPFDRARKNDILDAGLRIMAAVNERNRPRHPVDEHIGG
                     CKHVQFLAPGSDARHSRNAMAIHPGWFDRSPCGTGTSARMAQLHARGELPPHTEFVNES
                     FIGTRFTGRLLGTTEVGGVAAVVPEFSGRAWITGTANYLLDPRDPFPTGFVL"
     gene            complement(9643..10263)
                     /locus_tag="A4R43_RS07185"
                     /old_locus_tag="A4R43_07100"
     CDS             complement(9643..10263)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /locus_tag="A4R43_RS07185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R43_07100"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_113691600.1"
                     /transl_table=11
                     /translation="MSTVRPPRQDRAAATRARVIEAAYACLLEGGYAATTVGAVQQRAG
                     VARGTLLHHFPTRAALMAAVVDDIADRRVQVLDADPPAEGGWDGVVDLVWRDLQSPAFA
                     AALELWVASRTDPELRAALAPVQRRVFDSARRAIITHVGDRDPRVPTLAQFTIDLLTGT
                     AMSRVVDPDADVAVVVERWKRTLATLTGQSTNPVGNGSRGSSR"
     misc_feature    complement(12542..12557)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.25"
                     /tool="antismash"
     misc_feature    complement(12635..12649)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.37"
                     /tool="antismash"
ORIGIN
        1 gaccgggtgc ccgcggcggt ggtcacgctg ggcggtgagg gctgcctggt gctggaacgc
       61 ggccgcgaat ccttgcgcat tcccggaatc cgggtggagg cggtggacac cacgggcgcc
      121 ggtgacacgt tctgcggagt gctcgccgcc gcgctggccc ggcgctcggg cctcgccgag
      181 gcagcgcggc tggcctgcgt ggccgcggcg ctttcggtca cccgccgggg tgcgcaggcc
      241 gccgtcccgt cggcagccga agtcgccgcg gccggaggaa aggcaccatg acctacacgt
      301 tcgacccgct cgtgccccgg ccgatcgacc tcccgaccac tgtggacggt ccgctcgccg
      361 acctggacga ggcgaagatc ttcagcggac ccgccgatcc ggccgaccgc ccggcctggc
      421 gcgagcggct ccgggagtgg cgcgaggacg cgcgggcgcg gcacgcgtac acgggcacgg
      481 cctacgaacg gcccggcgcg gcctgggccg cgcgctgcta caccgtcgcg caggtgtggt
      541 tgtgggacga actgctgtat tcgttcgagg aaggccggtt cactccggcg cgctttctcg
      601 ccgacgcgcg gaagcgcttc ggcggcctgg acgcggtggt gctgtggcac gcctatccgg
      661 tgatcggcct ggacgaccgc aaccagtggg actactaccg cgacgtgccg ggcctgaccg
      721 agctgatcgg cgcgctgcac gtggcgggac tgcacgtgtt cgtcgactac aacccctggg
      781 acgtcggcac gcggcgcggt cgtgacgacc tcaccgaact cgccgagctg gtggccgact
      841 tcggggccga cggcgttttc ctcgacacgc tcaagaaggc cgaaccggag ttcgtcgaac
      901 ggctcgagca ggcgcgtccc ggcatcgtgc tggagggcga gtcgaagctg gccgtcgagc
      961 gcatcgaaga ccacgcctgc tcgtgggcgc agttcttcgc cgattcgccg gtgccggggg
     1021 tgctgcgcgc gcactggtac gaacggcgtc acatgcagca ccacatccgc cgctggcacc
     1081 gcgaccactc cgaggaactg cagtcggcgt ggctcaacgg ggtcggcgtg atggtgtggg
     1141 aggtggtgtt cggcgtgtgg gtcgggtggt ccgcgcgtga cgccgcgacc gtgcggcgga
     1201 tgaccaccgt gcagcgggcc gtgagttcgc tgctgctcga cggcgagtgg acgccgctcg
     1261 cggaactcgc gccggaggcg gaggccgccg ggatctacgc gtcccggtgg gaactcgacg
     1321 gcgttacctt gtggacggtc gtcaatcgcg gcgaccagga ctattcgggg cccgtggtgg
     1381 cggcggattc actgttcccc ggtgctgttc cggcgcgggg gatcggcgcg ttcgtccgcg
     1441 tcgagcctgg gacgccggag gcggaatggc tgcccggctt gcgggaaacc ctgcgcggcc
     1501 tgccgcacga ccccgacgcc agcttcccgc accggctcgc ggtccgcgtc cgtccacaac
     1561 ggagcacggg tacgcccgag ccggatgcgg tcgtcgtgcc cgccgggccc tatgtgctca
     1621 ccgtgcggta ccgcgccagg gaaaccggga tgtaccaggg tgcgccctat gtggacgagt
     1681 ggaagccgct gccgccccgg ctgcacgacg cgcggacgct gcagcgggac ggcgagctgg
     1741 ccaacgacgt ggccgtcgcg ctgaccgagg tgaccaacga gcagttcgcc gccttcctgg
     1801 ccgcgaccgg ctaccgtccc gcgcaaccac accgtttcct ggcacactgg ggcaacggcg
     1861 aatcgcctgg cgcgcccgtg acctacgtcg atctcgacga cgcacgtgcc tactgcgcct
     1921 ggcggggcgg caggctgccg accgaggacg aatggcagct cgccggggat cggctgcacc
     1981 ggggtgagcc cgcggtgtgg aactggaccg aaagcgagca ctcggacggc cgcacccggt
     2041 ttgtcctgct caagggcggc agcgactacc gggcggacgg cgccgactgg tacgtcgagg
     2101 gcgggcggca cgcgccggac cacagcgtga aactgctgct gccgggcctg ggccaggcgc
     2161 ggagcgcgac cgtcggcttc cgttgtgcct gggacatcac ggcttcctga acacctcgac
     2221 caggctcgtc gcgctgttct cgccgtgacc ggccgcgatg ccccggcgga ccgcggcgcg
     2281 gagggcttcg ggcagcgtgg tgtcgacacc ggcgtcctcg gcggtccgga cgacgtggtc
     2341 cacactggcc gccgccatgg tcagtcgttc ggcgtcgccc gcgtattcgc cggcgtcgat
     2401 gcgcggtgag tagaaggtga ggaactccgg gagcccgccg agcagggacg cggcgtgcgg
     2461 caggaagtca tcggcggaaa tgccgtgcgc gttcgccagg gcgagactgt gcagccagcc
     2521 cagcgtggtg gtccagaaca ggtccatggt gagctggtag taggcggcgg ccagcccggg
     2581 atcctcgccg cggtagtcgg tttcggtgag caccgacagg gcttcgcgat gggcgtcgaa
     2641 gacctcgcgg ggtccactgt agaaggtgta ggcccccggc tgcccgatct gcgggggcga
     2701 ggcctggacg ccaccggtga gatgcgcggc accgtgcccg gccgcccact tcgcggcggc
     2761 gcgggcctgc tcgggtgtgt cggaactgag gttgaccagg acccggccgg gcagcgtgtc
     2821 ggtggccggt tcgaggaggg tgtacatggc gtcgtagtcg gtcaggctca gcacgacgag
     2881 ttcgctggcg gcgaccgctt cgcgcacggt ggcggggcgg tgggcgcctt cggccaccag
     2941 ggcgtcggcc ttgctcgcgg tgcggttcca cacggtgacc cggtagccgt cgcggaggaa
     3001 ggtgcgtgcc atcgcccgcc ccatcggccc gagcccgagg accgtcacgt tcttgatcat
     3061 gtccccatcc tccgcccgct cgcggtggcg cggaagtacc gacatttttg tgcgtgcttc
     3121 ccgccgcccc gcgcggccgc gcgctatgaa tgtggctttc atagcgtctg gcgcaatgaa
     3181 agccacattc atagcgttct caccgtggtg cgttggagag cgagggccag tgcgggaccg
     3241 gagcggcgtc gggcggccgc gtagacgtag ccggtcagat gtggtcgagc gcggcgagca
     3301 gttcgtacga ccggcggcgg tccgcgtggt ggtacaccgg cgtggtcacc atgaattcgc
     3361 cggttccggt ggcctcgcgc acttcccgga gtcccgcggc cacggtttcc gggccgccgc
     3421 tgagcacact tcgggagcgg atcgcgatct cggcgcggtc gtccgccgtg taggggtagg
     3481 ccgccgcgtc ttccgggctc ggcagctgga tgcgggtgcc gcgaatccgg ctgagcacct
     3541 tcaaccgcgt ggagcccgcc agccactccg cccgctcgtc ggtttcggcg gcgatcacgg
     3601 acaggctgac cagcagcgcc ggcgactcgg catgctccga aggacggaag tgctcgcggt
     3661 aggcgtccgc ggaacgggcc gcttcgccgg ggttgaggtg gtgggcgaag gcgtaggcca
     3721 ggccgagcct gcccgccagt tcggcgctgg ccgccgagga accgagcagc cacaggtccg
     3781 gcacattgcc gccgagcacc ggaatcgcct tcaccggggc gtcttcggcc gggtgcagga
     3841 aggaccgcag ttcggccagc tgccgctcga agtccgccac cgtccgatct ggacggaccg
     3901 ccgccacggc cggcttcgca ccgcccggcg cgcggccgat gccgagatcg atgcgcccgg
     3961 ggtggaacgc ggccagcgtg ccgaactgct cggccaccac gatcggcgcg tggttgggca
     4021 gcagcacccc gcccgcgccg acgcgcagcc gccgggtggc gttggcgagg tggccgacca
     4081 ccaccgcggg cgccgagctg gcgacaccgc gcatcccgtg gtgctcgggt acccagtagc
     4141 ggtggaaccc cagcgagtcg gccagtttcg cgagatcgac cgtgttgtgc agggcctccg
     4201 acggcgtcgc gccctgcacg atcggcgagg tgtccagcac cgacaaccgc acgacggccc
     4261 cctccctttt ggcgcccctc agcacaccgt agtccacggc gggcggacgc tatagtgaac
     4321 ggcctaccgt gcgagcgtac gatttaccga acgaaaggcg gcggcagtgg agacctccga
     4381 ccagtcggtg cgagacctgc tggcggcgaa catcaagcgg gccagggcgg accgcgggtt
     4441 gtcgctgtcg gagctgtcgc gccggtcgtc gatcggcaag gcgacgctgt cgcagctcga
     4501 atcgggcgcc gggaacccga ccatcgagac ggtgttcagc ctctcgcgcg cgctggagct
     4561 gccgatctcg gacctgctcg aaggcgggcg cggcgagatg accgtcgtgc gcggcgacga
     4621 gcaggaggtg ctcgccgggg aggccgtgga acttcgcctg ctgcggcgga tcgaggacgg
     4681 cgggctgatc gtcgaggtgt acgaccagcg tgtgcgcggc acggcgcggc agcggtcgct
     4741 cgggcacgtg ggcaccgagc acaccatcgt gcaggcgggc aaactgcgcg tcgaggtcgg
     4801 tgacagcgcg gcggaactgg ggccgggcga ttcggtcgct ttcgacgcgt cgatcccgca
     4861 cagctaccag gcgatcggcg gcgaggtgcg ctcggtcctc ctgctgcagt accgcggcac
     4921 cgggccgcac gtgtgttgac aggcgcgcca ccggcggcct agcttcgaca gcgttcgggt
     4981 cagcgaacgg tggaggttcg gtgtacggaa cgcgggcggt ggtgatcggc gccgggatcg
     5041 tcggcgccgc ctgtgcccgc gagttgtccg ccgaaggctt ccgcgtgacc gtggtcgatc
     5101 gcgggcaccc cggcggcgcg accaccgcac acggcgaggg caacgtgctg gtctccgaca
     5161 agggccccgg ccctgaactg gaactggcca ggttgtcgcg ccggctgtgg ccggaaatcc
     5221 tggacgggct gccaggtacc gacgccgtcg agtgggacgc caagggcgga atcgtcgtcg
     5281 ccaccacgga agccggggct cgcgcgctga ccgggttcgc cgatggccag cggaacgccg
     5341 gcatccttgc cgaaccgctc gacgcggccg cgctcgccga agccgaaccg cggctgaccc
     5401 gcgaagtcac cgcggcgatc cactacccgg aagacgctca ggtgcagccc gtcgccgccg
     5461 ccaccgcact gctcgccgcc tcgggcgcca ccctgcgcac cggctgtaac gtgaccggac
     5521 cgctcaccga ttccgggtgc ctcaacggaa tccgcaccac ggccggtgac ctgcccgccg
     5581 acctggtggt gaacgccgcc gggccgtggg cgggagaggt ggccggtgtg ctcggcgcgc
     5641 cgatcgacgt ccggccgcgc cggggcgagg tgctggtgac cacgccgatg ccgccgaccg
     5701 tgttccacaa ggtctacgac gcggactacg tgggcgcggt cgtcagcgat gcggccgggc
     5761 tgcggacctc cgccgtggtc gagtcgacca aggcgggcac catcctgctc ggttcctccc
     5821 ggcgccaggt cggcttcgac ggccgcctgc acatcccggc gctctccgcg atcgccgcca
     5881 aggcgctgcg gttgttcccg ggactcgcgg gcgtgccggt gatgcgcgcg tacggcggtt
     5941 tccggcccta cgtaccggac cacctgccgg tgatcggcgc cgatccgcgg ctgccagggc
     6001 tgtggcacgc gaccgggcac gaaggtgccg ggatcggcct gtccgcaggc accggccgcg
     6061 tgctgcgcga cctggttgcc gggcggaaac cggaaatcga ggtcggaccg ttccgggtgg
     6121 accgcccggc ggtgctggga ggtgcccgat gagcccgcgg ccgatccggc tgaccatcga
     6181 cggcgaaccg gtgtccggaa tggacggtca gaccatcgcc ggggtgttgc tggcgtcggg
     6241 gcggaaagcg tggcgcaccg atccggccgg gcagccgcgc ggggtcttct gcggcatcgg
     6301 cgtctgcttc gactgcgtgg tgaccgtcaa cgcgctgcgc gacgtgcgag cctgccgacg
     6361 ccgtgcccgc gaaggcgacg acgtgcgcac gcagtccaga gtggatggtg agcggtgaag
     6421 cacgtggtgg tgatcggtgc cggtccggcc ggaatggcgg cggccgcggc ggcgttgcga
     6481 gccggggccg tggtgacggt gctcgattcg gccgaggagc ccgggggcca gtatcaccgc
     6541 aggccgctgg gcgcacggta ccgggcggcg aaggcgttcg accgggtgct cggccatccg
     6601 cgctgcacgt ggcgagcgga cacctcggtg tgggcgctgg aacgccgcga cggccgggcg
     6661 ccactggtgc acgcgttgcg cggggtcgcc gacggcgcgg accgggaagc cgtgtgcctc
     6721 gacccggatg ccctggtgct ggccaccggg gcacatgacc gggtgctgcc gttccccggc
     6781 tggacgctgc ccggggtgtt caccgcgggg gcggcgcagg cgctggcgaa gggcgagcgc
     6841 gtggccgtcg gccagcacgt cgtggtctcg ggtaccggcc cgttcctgct cccggtggcc
     6901 gcgtcactgc ttcaagtcgg agcccgcgtc gaagcggtac tggaagccaa ccagccacga
     6961 acgatcctcc ggggatgggc ccaagacctc agcgaactgg tgccgcacac cgggaaggcg
     7021 gcgcggctcg cccggtacgc ggccggtcag ctccggcacc gcgtgcccta ccggatgggg
     7081 caggcggtgg tcgaagcccg cggcgacggc cgcgtggagg aggtcgtggt ggcgcggctg
     7141 cgggcggact ggtcgatcgt gcccggaacc gaacgcacgc tgaccgcgga cgcggtgtgt
     7201 gtcggccacg ggttcagcgc gcagctcgaa ctccccgtgg ccgccgggtg cgagctggcc
     7261 ggtgacaccg tgcgcgtgga cggcgatcag cggaccaccc agcccggggt gttcgccgcc
     7321 ggggagatca ccgggatcgc cggtcagccg tccgctgtgg acagtggaac ggtggcgggc
     7381 tggcttgccg ccgggggcgc gcccgccgag atcgccggtg cattgcggcg gcgggatcgc
     7441 ggccgcgcct tcgggcgacg gctcgccgaa gcccatccga tcggtgacgg ttgggcgggc
     7501 tggctgcgac cggacacggt gatctgccgc tgcgaagccg tcgaccacgc gtgcctgacc
     7561 cgcgcgctcg ccgacccggt gaccgccggc gcgcgctcgg tgaagctgtc cacccgcgcg
     7621 gggctcggcc cctgccaggg ccggatctgc gggcccgccg tcgccgcgct caccaccgcg
     7681 gccggtgccg aagcggccgg tgcgcaccac cggccgatcg cccagcccat ccggctgggc
     7741 gagctggcga accagcgaac agaggagaac cgttgagcac caaggccgat ctgggcggcg
     7801 ttgtggtggc gaccgcgctg ccgtaccggg aagacgcaca agcaccagcc ggactcgcgg
     7861 tggactacga ccgctacgcc gagcactgcc gctggctgat cgacaacggc tgccacggca
     7921 tcgggccgaa cggctcgctc ggcgagtatt cctcgctcac cgacgaagaa cgccgccgcg
     7981 tggcgcagac cgccatcgag gccgtcggca atgacggcaa ggtggtggtc ggcgtgcacg
     8041 gtgtcggctc gcaccaggcg aagcactggg ccgaactcgc cgccgaggac ggcgcacacg
     8101 gggtgctgtg cctgccgccg acgatgtacc gggccaaccg aggcgaggtc gtggcgcact
     8161 tcgaagcggt ggcttcggtg ggcctgccgg tgatggtcta caacaacccg cacgacacca
     8221 aggtcgatct cacccccgac ctgctcggcg aactggctgg catcgagcac atcaccgcgg
     8281 tcaaggagtt ctccggagac gtgcggcggg tgctggagat caaggagcac gcgccggacc
     8341 tggcggtgat cagcggggcc gacgacgtcg cgctggagat gctgctgatg ggctccaccg
     8401 gctggttcgc cgggttcccg aacgtgttcc ccgccgagtc ggtcgagctg tacgaactgg
     8461 ccgtccgcgg cgagctgacg cgcgcccgcg cgcggtacga accgctggtg gccgcgttcc
     8521 gctgggactc gcgcaccgag ttcgtccagg cgatcaagct gggcatggac ctggccggcc
     8581 ggtacggcgg cccgtgccgg ccaccacgcg ggccgctggt cccggcacac gacgaacggg
     8641 tgcgggccga cctggcgcgc gcgatggacg cgctggcgag gcggtcctga tgcgctcgat
     8701 ccggacgctg accgcggtcg actcgcacac cgagggcatg ccgacccggg tgatcaccgg
     8761 gggagtggcg ccgatcccgg gtgacaccat ggccgcgcgc cgccgccact tcatcgacca
     8821 cctcgacgac ctccggcggt tcctggtcga cgagccacgc gggcacccgg cgatgagcgg
     8881 ggcgatcctg cagccgccgg cccggcccga cgccgactgg ggcgtgctct acgtcgaggt
     8941 cagcggggtg ctgccgatgt gcgggcacgg caccatcggg gtggccaccg tgctggtgga
     9001 gaccggcatg gtgccggtga ccgagccgga aaccgtggtg cggctggaca ccccggccgg
     9061 gctggtgcac gccagggtcg aggtgcgcga cggcgtggcc cagcgcgtga cgctgcgcaa
     9121 cgtcgactcg ttcgccgtcg aactcgacgc ggtcgccgac gtgccgggca tcggcgaggt
     9181 ccggtacgac ctggcctacg gcggcaactt ctacgccatc gtggacctgg cgagcatcgg
     9241 cctgccgttc gaccgggcac gcaagaacga cattctcgac gccgggctgc gcatcatggc
     9301 cgcggtcaac gagcggaacc ggccgcggca cccggtagac gagcacatcg gcggctgcaa
     9361 gcacgtgcag ttcctcgccc ccggctcgga cgcgcggcac tcacgcaacg ccatggccat
     9421 ccaccccggc tggttcgacc ggtcgccgtg cggcaccggc acgtcggcca ggatggcgca
     9481 gctgcacgcc cgcggtgagc tgccgccgca caccgagttc gtcaacgaat cgttcatcgg
     9541 caccaggttc accgggcggc tgctcggcac caccgaggtc ggcggggtgg cggcggtggt
     9601 gcccgagttc tccggccgcg cctggatcac cggcaccgcg aactacctgc tcgacccgcg
     9661 cgacccgttc cccaccgggt tcgtgctctg accggtcagc gtggccagcg tccgcttcca
     9721 ccgctcgacc accaccgcga cgtccgcgtc cggatcgacc acgcggctca tcgcggtgcc
     9781 ggtgagcagg tcgatggtga actgcgcgag cgtcgggacg cggggatcgc ggtcaccgac
     9841 atgggtgatg atggcgcgtc gcgcggagtc gaacacgcgc cgctgcaccg gggcgagcgc
     9901 ggcccgcagc tcgggatcgg tgcgcgaggc gacccacagc tcgagcgccg cggcgaacgc
     9961 cgggctctgc aggtcgcgcc agaccaggtc gaccacgccg tcccagccgc cttccgccgg
    10021 cgggtcggcg tcgagcacct ggacgcggcg gtccgcgatg tcgtcgacca ccgcggccat
    10081 cagcgccgcg cgggtgggga agtggtgcag cagcgtgccg cgggccacgc ccgcccgctg
    10141 ctgcacggcg ccgaccgtgg tggccgcgta gccgccttcc agcaggcagg cgtacgccgc
    10201 ttcgatcacc ctggcgcggg tggccgccgc gcggtcctgc cgtggtgggc gcactgtgga
    10261 caccgggcca ctgtaatggc acgatcgccg aacagtctga ccggactggt ttgcggaagg
    10321 tgagcgatga gcaggtggcg taccgccagg acgctggccg gcgcgctggt gcgtggcgcg
    10381 ttcgcgcgct cgcgcggtgc ggccccggcc ggtctgccgg aggtgcccgc gcccgccgga
    10441 atcgacggtt acgcgcgcca cgtgaccgcg cggggtgcgg tcgccgccga acccgccgcg
    10501 gtgaccgcgc tcgcccgcga cctcgaccgc acccacgagt ggctgaccct gcacgcggcc
    10561 tggcggggtg aacggccgga gcggatcgag ccgggcgcgg agttcgtgca gcagatccgg
    10621 ctgatggaca tccccgcgca ggcgaggtgg gtggtcgaac gggcggacga cgacgggttc
    10681 gcgttgcgcg ggaccggtcc gatgggcatc acgctgggcc tgtggtgcac ggtccgcccg
    10741 gcagcggacg gctcggtggt tcggctggac gccgcgctgg acgggtcacc ggtgcgtggt
    10801 cccgtcggtt cgacggccgt gcgcagtgtc gagcaggcac tgaccgactc actggccgcg
    10861 ctcgcggggc tcgtgggcga cgccccggtg cggcggctgt ccgtgccgag cgagccgatt
    10921 caccatgagc ggaccggaaa gctgctcgat ccgcacaccc ccgtggtggt cggtgtcggg
    10981 caggtggtca accggacgcc ggacctgtcc gcaccacgcg agccggtcga tctggccgtc
    11041 gaagccttgc gcgcggccgc cgccgactcg ggcgcggagg tggatctgct ggcgcaggcc
    11101 gatctggtct acgcggtgcc gagtgcttcg tggagttatc ccgaccaggc gggcgcggtc
    11161 gccgggctgg tcggtgcttc gcgcgcgacc accgtgcaga ccagcgccca gggcggcgac
    11221 ggcggacagc tcgcggtcaa cgacgccgcc caggagatcg tcgacggccg cgcggacatc
    11281 gccgtcatct cgggtgccga ggccggtgcc acgatggccg cgctgaccgg tgaaccccgc
    11341 tggacccggc agcccgcgtc ggccgcgccc caccggaaga tcgggctgga ccggcccgcc
    11401 aacaacgagg ccgagacggc cgtgggactc ggcgcgccga tcttcgtcta cccgctgctg
    11461 gagtcggcgc tgcggggtcg cgagggcacc gcgcccggcg agcaccgcgc ccgcatcgcc
    11521 gcgctctggg cgcggatgtc gcaggtggcc gccgaaaacc cgtacgcgtg ggaccggacc
    11581 gcgcgcacgg cggaggagat cgcgacgccg ggtgagcgca accggtgggt cagcgacccg
    11641 tacaccaagc tcatgtgcgc caatctgcag gtcgatctcg cggccgcggt gatcgtgacg
    11701 agcgcggcgg ccgcgcgggc ggcgggcatt ccgcaggacc gctgggtttt tgtgcacgcc
    11761 ggggcatcgg ccaccgagga gtggtttgtc tccgaacgcg cttcgctcgc cgaatccccg
    11821 gccatcgcgg ccgccggggc cgcggcgctc gggcacgcgg gcgtcaaacc ggccgacctt
    11881 ggcccggtgg acctctactc gtgcttcccg tccgccgttc agctggcggc caacgcgctc
    11941 ggcctgccgt gggacgaccc ggcacgcccg ctgaccgtca ccggcgggct cacctcggcg
    12001 ggcgggccgg gcaacaacta cggaacgcac gcggtggcgt cgatcgtgcc gcggctgcgg
    12061 gccgcgccgg acgaggtcgg gctcaccacg tcgctgggct ggtacgcgac caagcacgcg
    12121 gtcggcgtct actccgcgac gccgccggac cgggcgttcg cacacctgcg accggcgttc
    12181 ccccggccgg ccacccggcc cgcactgacc gaatgggacg gtgccggggt ggtcgaagcg
    12241 gcaacgctgg tgcaccggcg tgacggcgaa cccgaagcga tgctgctgag cgtgctcacc
    12301 gacgacggcg cgcgtgtcct gctgcggcgc gaggaaccgg aactgcacgg cgtcgatctt
    12361 gtgcgccgcc gagtccggct ggctgacggc cggatcgagg tgctcgacga ctcgcgccac
    12421 gaactgccac cgccgccacc cgcgcccgtg cgcaccgaac gccgcggcac cgggatctcg
    12481 gtgatcacgc tcgaccggcc cgccgtccgc aacgcggtga accggcgcct ggcgctcgcg
    12541 ctggaacagg cggtgaacga cgccgaagcc gatccggccg tccgcgtgct ggtgctgacc
    12601 ggtgccgggg gcacgttctg cgctggcatg gacctggccg aggcgaaccg cggtgccgtg
    12661 ccggtcaccg accgccgcgg cccgctcggg ctgaccgccg agccacccgc caagccgacc
    12721 atcgccgccg tcgaaggcgc ggcgctcgcg ggcgggttcg agctggcact gtgcgc
//
