LOCUS       NZ_LWAI01000043         3741 bp    DNA     linear   CON 07-APR-2024
DEFINITION  Priestia endophytica strain 3618_1C 3618_1C_contig_47, whole genome
            shotgun sequence.
ACCESSION   NZ_LWAI01000043
VERSION     NZ_LWAI01000043.1
KEYWORDS    WGS; RefSeq.
SOURCE      Priestia endophytica
  ORGANISM  Priestia endophytica
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Lekota,K.E., Mafofo,J., Rees,J., Muchadeyi,F.C., Madoroba,E. and Van
            Heerden,H.
  TITLE     Comparison of Bacillus endophyticus and B. anthracis characteristics
            using whole genome sequence analysis and microbiological analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Lekota,K.E., Mafofo,J., Rees,J., Muchadeyi,F.C., Madoroba,E. and Van
            Heerden,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2016) Biotechnology Platform, Agricultural
            Research Council, 100 Soutpan Road, Pretoria, Gauteng 0110, South
            Africa
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAY-2015
            Assembly Method                   :: CLC Genomics workbench v. 7.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 330.0x
            Sequencing Technology             :: Illumina HiScan SQ
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003269975.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 05:04:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,413
            CDSs (total)                      :: 5,355
            Genes (coding)                    :: 5,223
            CDSs (with protein)               :: 5,223
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 44 of 132
            Pseudo Genes (incomplete)         :: 88 of 132
            Pseudo Genes (internal stop)      :: 43 of 132
            Pseudo Genes (multiple problems)  :: 35 of 132
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:25:48
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3741
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LWAI01000043.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..3737)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (195-263). E-value: 2.8e-26. Score:
                     83.5. Matches aSDomain: nrpspksdomains_A4U60_RS18055_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (273-577). E-value:
                     5.2e-142. Score: 466.1. Matches aSDomain:
                     nrpspksdomains_A4U60_RS18055_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (586-732). E-value: 4.6e-37.
                     Score: 119.2. Matches aSDomain:
                     nrpspksdomains_A4U60_RS18055_TIGR01720.1"
                     /NRPS_PKS="Domain: Condensation_DCL (748-1047). E-value:
                     3.9e-113. Score: 369.5. Matches aSDomain:
                     nrpspksdomains_A4U60_RS18055_Condensation_DCL.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255753.1"
                     /locus_tag="A4U60_RS18055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4U60_17895"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_142674266.1"
                     /sec_met_domain="AMP-binding (E-value: 3.7e-46, bitscore:
                     143.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.5e-17, bitscore:
                     49.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.3e-74, bitscore:
                     235.7, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIGRPLPNVQVYVLDRNLLPVPIGVTGELYIGGAGLAKGYLNRP
                     ELTSERFIRHPFKEGERLYRTGDLVRYLPDGNLDYLGRMDNQVKIRGFRIELGEIEATL
                     QEDFLVKEAVVMVRENQPGDKRLVAYVVGEGSKEEWREYLKTKMPNHMVPSYFVEMEEL
                     PLTINGKIDRKSLPIPDYQGTQEGYVAPRNEREQILSVIWERVLGSKRIGIHDNFFEIG
                     GDSILSIQIVSRVKQAGLQLTPKQIFEHQTIAELAQVVKEEQGVQAEQGIVTGDMILTP
                     IQQSFFAQNHSNPHHWNQSMFFRTKERLDIVSLEKSVRNLLLHHDALRLRYECLPDGAW
                     KQWNEGIEEQSTLTVISLDEVPQTDWYQVVQKEINIAQESLNLHAGPLMRMVYFDEGEK
                     KTGRLFWSIHHLAVDGVSWRILLEDLQTAYTQAIQEQKIQLPMKSTSFKAWSEKLHHYA
                     ESRISQEVLDYWEQQSKQEVVMPPVDVTVNDSINEVTEEITVVLDEKETRTLLQEVLST
                     HRAQINEVLLAALVQATAVWTGQPILTVDLEGHGREEIIEDVDLSRTVGWFTSIYPVHL
                     NITGADTPIAALKAVKEQVRKIPNKGVDYGILRYLNATMCEQLGSQHTPFISFNYLGQF
                     DQMFSDDAMFVPENGFKRLDHASGSKRSHLIDVIGIVTDGKLQFTWVYNVGQFAKSTIQ
                     SIAENLLHQLSRLIQSSDGESALTVSDFAMANLNQVGLTKVLSKMNRGKNYPITDLYPL
                     SPLQEGMIFHTLHDQEDEHVAPYIVQLSFMIRGELDIPTFEQAWKLVIQQHEIFRSAFV
                     WDEIEEPLQVVYDNIPFKVNEADWRTLTAEEKEAKRKVFLALDRKQAFLFDKAPLMRVT
                     VIQEGEEEYRVVWTHHHILLDGWSLPLVFTELLTVYQKRMNGETVNLPKSLPYKKYIQW
                     LREQNQEQAEQFWREKLKGFTAPTLLGLESKEQEKGYTEKVTHLSEEQTQALQSWAKRN
                     KLTLSTIIQGAWAYLMSRYSGENDIVFGVTSSGRPTEIIDVEHIVGPFITTSPTRIQLI
                     DDTKVGDWLQKIQEEELERRQYEYASLTEIQGWSEVPRGTPLFNSLYVFENYPVKKESS
                     GNLEIGELEGVEQTHYPLGLAVVPGIQLSLKLMYDRSKFNRLTIERMLVHLRQVLKQMI
                     ENVDQTLSELVYVTETEQRQLLEEWNDNAISYPRESVIHKLFEDQVNRTPDKVAVVDEN
                     QQLTYRELDEKANQLAHYLQK"
     source          1..3741
                     /collection_date="2009-05-01"
                     /country="South Africa: Kimberly"
                     /db_xref="taxon:135735"
                     /host="Tragelaphus strepsiceros"
                     /mol_type="genomic DNA"
                     /organism="Priestia endophytica"
                     /strain="3618_1C"
                     /submitter_seqid="3618_1C_contig_47"
     gene            complement(<1..>3741)
                     /locus_tag="A4U60_RS18055"
                     /old_locus_tag="A4U60_17895"
     region          1..3741
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..3741
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..3741
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:3737](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..3737)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(433..555)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4U60_RS18055_0012"
                     /evalue="3.00E-22"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="1101"
                     /protein_start="1060"
                     /score="71.1"
                     /tool="antismash"
                     /translation="QEEELERRQYEYASLTEIQGWSEVPRGTPLFNSLYVFENYP"
     aSDomain        complement(595..1491)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4U60_RS18055_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="3.90E-113"
                     /label="A4U60_RS18055_Condensation_DCL.1"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="1047"
                     /protein_start="748"
                     /score="369.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHILLDG"
                     /tool="antismash"
                     /translation="DLYPLSPLQEGMIFHTLHDQEDEHVAPYIVQLSFMIRGELDIPTF
                     EQAWKLVIQQHEIFRSAFVWDEIEEPLQVVYDNIPFKVNEADWRTLTAEEKEAKRKVFL
                     ALDRKQAFLFDKAPLMRVTVIQEGEEEYRVVWTHHHILLDGWSLPLVFTELLTVYQKRM
                     NGETVNLPKSLPYKKYIQWLREQNQEQAEQFWREKLKGFTAPTLLGLESKEQEKGYTEK
                     VTHLSEEQTQALQSWAKRNKLTLSTIIQGAWAYLMSRYSGENDIVFGVTSSGRPTEIID
                     VEHIVGPFITTSPTRIQL"
     CDS_motif       complement(604..699)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4U60_RS18055_0011"
                     /evalue="1.20E-14"
                     /label="C5_DCL_263-294"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="1044"
                     /protein_start="1012"
                     /score="46.6"
                     /tool="antismash"
                     /translation="DIVFGVTSSGRPTEIIDVEHIVGPFITTSPTR"
     CDS_motif       complement(931..969)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4U60_RS18055_0010"
                     /evalue="4.70E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="935"
                     /protein_start="922"
                     /score="9.8"
                     /tool="antismash"
                     /translation="PYKKYIQWLREQN"
     CDS_motif       complement(1015..1080)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4U60_RS18055_0009"
                     /evalue="1.40E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="907"
                     /protein_start="885"
                     /score="36.7"
                     /tool="antismash"
                     /translation="HHHILLDGWSLPLVFTELLTVY"
     CDS_motif       complement(1297..1413)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4U60_RS18055_0008"
                     /evalue="1.20E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="813"
                     /protein_start="774"
                     /score="46.5"
                     /tool="antismash"
                     /translation="PYIVQLSFMIRGELDIPTFEQAWKLVIQQHEIFRSAFVW"
     CDS_motif       complement(1447..1485)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4U60_RS18055_0007"
                     /evalue="2.70E-05"
                     /label="C1_dual_004-017"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="763"
                     /protein_start="750"
                     /score="16.8"
                     /tool="antismash"
                     /translation="YPLSPLQEGMIFH"
     aSDomain        complement(1540..1977)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4U60_RS18055_TIGR01720.1"
                     /evalue="4.60E-37"
                     /label="A4U60_RS18055_TIGR01720.1"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="732"
                     /protein_start="586"
                     /score="119.2"
                     /tool="antismash"
                     /translation="ALKAVKEQVRKIPNKGVDYGILRYLNATMCEQLGSQHTPFISFNY
                     LGQFDQMFSDDAMFVPENGFKRLDHASGSKRSHLIDVIGIVTDGKLQFTWVYNVGQFAK
                     STIQSIAENLLHQLSRLIQSSDGESALTVSDFAMANLNQVGL"
     aSModule        complement(1540..3150)
                     /domains="nrpspksdomains_A4U60_RS18055_PCP.1"
                     /domains="nrpspksdomains_A4U60_RS18055_Epimerization.1"
                     /domains="nrpspksdomains_A4U60_RS18055_TIGR01720.1"
                     /incomplete
                     /locus_tags="A4U60_RS18055"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(1903..1965)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4U60_RS18055_0006"
                     /evalue="9.70E-11"
                     /label="NRPS-E6"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="611"
                     /protein_start="590"
                     /score="33.8"
                     /tool="antismash"
                     /translation="VKEQVRKIPNKGVDYGILRYL"
     aSDomain        complement(2005..2916)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4U60_RS18055_Epimerization.1"
                     /evalue="5.20E-142"
                     /label="A4U60_RS18055_Epimerization.1"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="577"
                     /protein_start="273"
                     /score="466.1"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLAVDG"
                     /tool="antismash"
                     /translation="TGDMILTPIQQSFFAQNHSNPHHWNQSMFFRTKERLDIVSLEKSV
                     RNLLLHHDALRLRYECLPDGAWKQWNEGIEEQSTLTVISLDEVPQTDWYQVVQKEINIA
                     QESLNLHAGPLMRMVYFDEGEKKTGRLFWSIHHLAVDGVSWRILLEDLQTAYTQAIQEQ
                     KIQLPMKSTSFKAWSEKLHHYAESRISQEVLDYWEQQSKQEVVMPPVDVTVNDSINEVT
                     EEITVVLDEKETRTLLQEVLSTHRAQINEVLLAALVQATAVWTGQPILTVDLEGHGREE
                     IIEDVDLSRTVGWFTSIYPVHLN"
     CDS_motif       complement(2008..2058)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4U60_RS18055_0005"
                     /evalue="1.00E-09"
                     /label="NRPS-E5"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="576"
                     /protein_start="559"
                     /score="30.2"
                     /tool="antismash"
                     /translation="DLSRTVGWFTSIYPVHL"
     CDS_motif       complement(2461..2526)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4U60_RS18055_0004"
                     /evalue="3.90E-11"
                     /label="NRPS-E2"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="425"
                     /protein_start="403"
                     /score="35.1"
                     /tool="antismash"
                     /translation="LFWSIHHLAVDGVSWRILLEDL"
     CDS_motif       complement(2740..2814)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4U60_RS18055_0003"
                     /evalue="3.40E-03"
                     /label="C2_LCL_024-062"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="332"
                     /protein_start="307"
                     /score="9.8"
                     /tool="antismash"
                     /translation="RLDIVSLEKSVRNLLLHHDALRLRY"
     aSDomain        complement(2947..3150)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4U60_RS18055_PCP.1"
                     /evalue="2.80E-26"
                     /label="A4U60_RS18055_PCP.1"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="263"
                     /protein_start="195"
                     /score="83.5"
                     /tool="antismash"
                     /translation="EQILSVIWERVLGSKRIGIHDNFFEIGGDSILSIQIVSRVKQAGL
                     QLTPKQIFEHQTIAELAQVVKEE"
     CDS_motif       complement(3433..3498)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4U60_RS18055_0002"
                     /evalue="6.10E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="101"
                     /protein_start="79"
                     /score="40.5"
                     /tool="antismash"
                     /translation="YLGRMDNQVKIRGFRIELGEIE"
     CDS_motif       complement(3580..3669)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4U60_RS18055_0001"
                     /evalue="1.80E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="A4U60_RS18055"
                     /protein_end="52"
                     /protein_start="22"
                     /score="58.6"
                     /tool="antismash"
                     /translation="PIGVTGELYIGGAGLAKGYLNRPELTSERF"
ORIGIN
        1 atttctgaag ataatgggcc aactggtttg ctttttcatc caactctcga tatgtcaact
       61 gctgattttc atccaccact gccaccttat caggtgtacg attcacttga tcttcaaaca
      121 atttatgaat cacactctca cgtggatatg atatagcatt atcattccat tcttctaata
      181 gttgtctctg ttctgtttcc gtaacataca ctagctccga cagagtttga tcaacgtttt
      241 caatcatttg ctttaacact tggcgtaaat gcaccaacat ccgttcaatg gttaatctat
      301 taaatttact acgatcatac attaacttta acgataactg gattcccgga acaacagcca
      361 atcctaatgg atagtgggtt tgctctactc cttccaactc gccaatttct agatttcctg
      421 aagactcctt tttcactgga tagttttcaa acacatacaa actattgaac agcggtgttc
      481 ctctaggaac ttcactccat ccctgaatct ctgtcaaaga agcatactca tattgtcttc
      541 tttctagctc ttcctcttgt atcttttgca accaatcccc tacttttgta tcatctatca
      601 attggattcg cgtcggtgat gtggtaataa aaggacctac gatgtgttca acatcgataa
      661 tctccgtagg gcgccctgag ctagttacac caaagacaat gtcattttct ccactatacc
      721 ggctcatcag atacgcccac gcaccttgaa ttatcgtact caaagtgagc ttattgcgct
      781 tcgcccagct ttgtaaagct tgggtctgct cttctgataa atgagtaacc ttctctgtgt
      841 aacctttttc ttgctcttta ctctctaagc ccagcagggt tggcgctgta aaacctttaa
      901 gtttctctct ccagaattgt tccgcctgct cctgattctg ttctcttagc cattggatat
      961 atttcttgta tggcaatgac ttaggtaaat tcacagtttc tccattcatt ctcttctgat
     1021 acactgtaag caattcggta aagaccagtg gtaggctcca tccatccaac agaatatggt
     1081 gatgtgtcca aacaacccga tattcttcct caccctcttg aatgacagtc acccgcatca
     1141 atggcgcttt atcaaaaaga aaagcttgtt ttcgatccaa tgccaaaaag acttttcttt
     1201 tcgcctcttt ttcttcagca gtcagcgtac gccaatctgc ttcattcacc ttaaatggaa
     1261 tgttgtcata caccacttgt aaaggctctt caatttcatc ccaaacaaat gctgaacgaa
     1321 aaatctcatg ctgctgaatc accaatttcc acgcctgctc gaaggtagga atatccaact
     1381 ctcctctaat catgaaactt aattgcacta tataaggcgc tacatgttca tcctcttggt
     1441 catgcagtgt atgaaaaatc ataccttcct gcaaaggcga tagtggatac aaatcggtaa
     1501 tcggatagtt ctttccacga ttcatcttgg atagtacttt tgtcaaacct acttggttaa
     1561 gattagccat cgcaaaatcc gaaacggtca gtgcagattc tccatccgaa gattgaatca
     1621 atctgctcag ttgatggagc agattctctg caatactttg aatagttgat ttcgcaaatt
     1681 gtccaacatt gtacacccaa gtgaactgaa gttttccatc cgttacaatc ccaattacat
     1741 cgattaaatg cgatcgcttg gacccagaag catgatctaa gcgtttaaat ccgttctctg
     1801 gaacaaacat tgcatcgtca gaaaacattt ggtcaaattg tccaagatag ttaaaactaa
     1861 tgaacggtgt atgttgagac cccaattgct cacacattgt tgcgttcaag taccgcaaaa
     1921 tcccatagtc aacaccctta ttaggaattt tgcgcacttg ttctttaact gcttttaatg
     1981 ctgcaattgg cgtgtcggca ccggtaatat ttaagtgaac aggataaata cttgtaaacc
     2041 atcccacggt tctcgatagg tcaacgtctt caataatctc ttctcgacca tgcccctcta
     2101 gatccacggt cagtatcggc tgtcctgtcc atactgccgt agcttgtacc aatgctgcta
     2161 atagtacttc attaatttga gcacgatgag tgcttaaaac ctcttgcaat aacgtacggg
     2221 tctctttctc atctagtacc acggtaattt cctcagttac ttcgtttatt gaatcattaa
     2281 cagtaacatc cactggtggc atcactactt cttgtttgga ctgctgctcc cagtaatcaa
     2341 gcacttcttg tgatattcta gattcagcgt aatgatgtaa tttctccgac catgctttaa
     2401 aggatgtact cttcataggt aattgaattt tctgttcctg aattgcttga gtatatgcgg
     2461 tttgcagatc ttccaataaa atccgccaag aaacaccatc tactgctaag tgatgaattg
     2521 accaaaataa tcgtccagtc ttcttctcac cttcatcaaa atataccatt ctcattaacg
     2581 gaccggcatg taaattcaaa ctttcttgtg caatattaat ctctttttga accacttgat
     2641 accaatctgt ttgcggcact tcatctaatg aaattacagt cagcgtcgat tgttcttcta
     2701 ttccttcatt ccactgtttc caagctccat caggtaaaca ttcatatctt aaacgcaggg
     2761 catcatgatg aagcagaagg ttacgcactg acttctccag tgatactatg tccagtcgct
     2821 cttttgttct aaagaacatg gattgattcc aatgatgtgg gttagaatga ttttgtgcaa
     2881 aaaagctttg ctgaataggt gtaagaatca tatctccagt tacaattccc tgttcggctt
     2941 gtaccccctg ttcttctttt actacttgcg ccagctcggc aatcgtttgg tgttcaaata
     3001 tttgttttgg agtcaactgt aaacctgctt gcttcacacg cgagacaatc tgaatgctga
     3061 gaatagagtc tccaccgatt tcaaaaaagt tgtcatgaat gccaatacgt tttgaaccca
     3121 gcacacgttc ccaaattaca gaaagtattt gttcacgttc atttcttgga gccacgtaac
     3181 cttcttgtgt cccttgataa tctggtatag gcaaagattt ccgatcaatc tttccattta
     3241 tggtcaatgg taactcttcc atttcgacaa agtaggaagg aaccatatgg tttggcattt
     3301 ttgttttgag atactcgcgc cattcttcct tactcccttc tcccactaca taagctacca
     3361 gcctcttatc tcccggctgg ttttccctaa ccatcacaac tgcttctttt accaagaagt
     3421 cctcttgtaa ggtagcctca atttctccaa gttcaatccg aaatcctcta attttcactt
     3481 gattatccat tcttcctaga taatctaaat taccatcagg aagatatctc acaagatctc
     3541 ccgtccgata taatctctct ccttctttga atggatgacg aataaatcgt tcggacgtta
     3601 actctgggcg attcaagtat cccttcgcta aacctgcccc accaatatag agctcacccg
     3661 ttacaccaat tggaacaggt aacaaatttc tatccaatac atagacttga acgttcggta
     3721 acggacgccc aatggttacc c
//
