LOCUS       NZ_QLTT01000019        10819 bp    DNA     linear   CON 17-JAN-2024
DEFINITION  Lentzea atacamensis strain DSM 45479 Ga0215649_119, whole genome
            shotgun sequence.
ACCESSION   NZ_QLTT01000019
VERSION     NZ_QLTT01000019.1
KEYWORDS    WGS; RefSeq.
SOURCE      Lentzea atacamensis
  ORGANISM  Lentzea atacamensis
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Lentzea.
REFERENCE   1  (bases 1 to 69157)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing
            the most valuable type-strain genomes for metagenomic binning,
            comparative biology and taxonomic classification
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 69157)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2018) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Expected Final Version            :: No
            Genome Coverage                   :: 111.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/17/2024 11:04:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,181
            CDSs (total)                      :: 9,101
            Genes (coding)                    :: 8,972
            CDSs (with protein)               :: 8,972
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 6 (5S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 27 of 129
            Pseudo Genes (incomplete)         :: 107 of 129
            Pseudo Genes (internal stop)      :: 5 of 129
            Pseudo Genes (multiple problems)  :: 10 of 129
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:43:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 34603
            Orig. end                         :: 45422
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QLTT01000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10819
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10819
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10819
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5819](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5819)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(246..257)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     gene            656..1348
                     /gene="hemQ"
                     /locus_tag="C8D87_RS44440"
                     /old_locus_tag="C8D87_11939"
     CDS             656..1348
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="C8D87_RS44440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D87_11939"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_109642599.1"
                     /transl_table=11
                     /translation="MARLNYNELNDTIRYTMWSVFRVEPGKLPEDRSAAASETQNYLDA
                     LEGKGVVVRGVYDVAGLRADADFMIWWHAEEIEQVQAAYTGFRRTPLGKVSTPVWSQVA
                     LHRPAEFNRSHIPAFLANEEPRKYVCVYPFVRSYEWYLLPEEDRRKMLADHGKEARDFP
                     DVRANTVASFALGDYEWMLAFEADELHRIVDLMRHLRGTEARLHVREEIPFYTGTRVPA
                     AELVTNLP"
     gene            complement(1398..4472)
                     /locus_tag="C8D87_RS44445"
                     /old_locus_tag="C8D87_11940"
     CDS             complement(1398..4472)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /locus_tag="C8D87_RS44445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D87_11940"
                     /product="S8 family serine peptidase"
                     /protein_id="WP_112232655.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.8e-62, bitscore:
                     203.4, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRGNVKVLGAAFAALALTAGTATAASAAPQPTGPQRAVDEVTLVT
                     GDKVLLGQAQQVVAIQPGEGRKGMTFKTHRGADGHTMVVPTDAFGPLAAGRLDRRLFDV
                     TTLLEFGYRDRMPLIVDGQARLGARSSADWAALRAEAASGDKIWLDGIRKPSLDRSVAQ
                     IGAPAAHQRGITGKGVKVAIVDTGVDENHPDLKGQQIAEKNFTSDPDTTDRVGHGTHVG
                     ATVASAGSQYGGVAPGAQLLDAKVCVEFGCQESWILDGMRWAAEQGAQVVNMSLGGGDT
                     PEIDPLEQAVNDLTAKYGTLFVIAAGNSGGPGTVSSPSTAEAALSVGAVDRDDKLAYFS
                     SRGPRTGDSGLKPEVTAPGVEIVAAKAGSTEHVSASGTSMATPHVAGAAALLKQQHPEW
                     TPAQLKSALATTAKPAPGVSPFDQGAGRIDVAKAINQVLTADVVAVNLGLHEWPHGDDK
                     PETRSITYSNPTDADITLDVKITSGAPQGMFSLSASMVTVPAKGTAAVKVTGDTRVGTQ
                     DAAYTGEVVATSGTHTVRTLVGVEREVESYTLTIDMIGRDGQDPVDALTFVSGLDIPTS
                     KVVFGGGDQKIRLPKGTYVVDSSISSPNGMDMLFHPTVKVTGDLAIKQDARLAQPLSVT
                     VPEPTGDFVVGELSYRRAYGDRVLQAGLIAFGGFDGISVAQVGGPLSPKELASMATLHA
                     NSAAEANVYYRLSYPVAGSLPNGLTRAPGKKDLARVEATIGGLRPDSRALKGEFPTSVH
                     GVGGGSALYQVPAGSKAVDYVTTEDLRWQSIVSITHANGSREEAVSGFRTYEAGKTYQE
                     RENQPVFSPALPESPYPYAQRNGTQLFIGVPMLSDASSGQGGSTVDSFRAALYRNGVLI
                     GEGTGGNFQNVPLPEGDFRAEASLDRSSQLEFSTKVSAAWTFRSKGTPGVTDTIPLSVV
                     RFAPRLDDNGAVPAGTLQRVGLVTQSQGDTGKVRITGVQYSHDDGKSWRNAGVAGNSAL
                     IYHPAGAKWTSLKATAKDAKGNTVEVTVIHAYKVS"
     misc_feature    4437..4446
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    4534..4548
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.16"
                     /tool="antismash"
     gene            complement(4637..5008)
                     /locus_tag="C8D87_RS44450"
                     /old_locus_tag="C8D87_11941"
     CDS             complement(4637..5008)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671914.1"
                     /locus_tag="C8D87_RS44450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D87_11941"
                     /product="hypothetical protein"
                     /protein_id="WP_233442581.1"
                     /transl_table=11
                     /translation="MAEIIDRNDPSAARERLAAAQAELLNALLANGPAPAGFDEQRLDV
                     ERRALLSKRRGIVRMLGPAVADELGDRFRPLFDAYALENPRRAGSRARADAAAFAEWCR
                     AAGELSAKRKARWKFWQSN"
     gene            complement(5001..5819)
                     /locus_tag="C8D87_RS44455"
                     /old_locus_tag="C8D87_11942"
     CDS             complement(5001..5819)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671915.1"
                     /locus_tag="C8D87_RS44455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D87_11942"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_112232657.1"
                     /sec_met_domain="DUF692 (E-value: 3.7e-86, bitscore: 280.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGIADLGVGIGWRSEIDLTVERLPGVDFVEVVAENLHIGHLPES
                     VTLLLERGVPVLPHAVSLSLGGADPVDLGRVEHLGKVAEAVGAPMVSDHVCFVRAGGLD
                     SGHLMPVPRTRDALDVLVANVELAQRNLPVPLALENVAALLEWPDGELSEGQFLAELVE
                     RTDCWLLVDVANLYANARNIGTDPERFLDEIPLERLAYVHVAGGVDHGDVYHDTHAHAV
                     LPEVLGVLDELCSRVKPPGVLLERDDNYPSDTEIASELAAIRAVVDKHRG"
     gene            complement(5819..6601)
                     /locus_tag="C8D87_RS44460"
                     /old_locus_tag="C8D87_11943"
     CDS             complement(5819..6601)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="C8D87_RS44460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D87_11943"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_170166822.1"
                     /transl_table=11
                     /translation="MTTQGTQLSAEQVGYLTAGPGRAAETALARLIDAGLVRVSRDGLV
                     SAVHQNNHGATTAIEARILSYARTPVRFDTIVRSAAHSTEMSMLHQHLLDRKLLQRPRR
                     RNEGWWFSLVIAVVLVLLGFVALEFLLGVPIAVLFFLWQRGRKAITGAGREALLRVTAT
                     DRVHAVAVYGFCGRVNRRPVADLFELSQSIVRMLPLKQNRKRRSSSDGGGSGSSCSSCG
                     SGCGSSSCSSSSGSSCSSSSSSSCGGSSCGGGGGGGGD"
     misc_feature    complement(6607..6621)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.17"
                     /tool="antismash"
     gene            complement(6625..7455)
                     /locus_tag="C8D87_RS44465"
                     /old_locus_tag="C8D87_11944"
     CDS             complement(6625..7455)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="C8D87_RS44465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D87_11944"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_146772105.1"
                     /transl_table=11
                     /translation="MAKSSTPQANWAAEEIGFLAGGPGRAAEAALARLMDGGLVRFSRE
                     GLVTAVHQNGHGATSPLEASILAGLSGGGRPIGLVVGPAAHSQEMGALHRNLVEKALVH
                     RQWGKTSGGRVALRVLLVLLGIAFLAAALFVDLNLLAVTLVAWFVAFLLRDKKMLTPIG
                     RGILGHAQRNPRSRVDAVALHGLRGVVGRRRVADLYGLPPGILYPPPVHHHGKQKSHSD
                     SGGCGGVHEHHNCASSSSCSSSSCSSSSCSSSSSSCSSSSCGSSSSSSCSSSSS"
     gene            complement(7548..8420)
                     /locus_tag="C8D87_RS44470"
                     /old_locus_tag="C8D87_11945"
     CDS             complement(7548..8420)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="C8D87_RS44470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D87_11945"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_109642609.1"
                     /transl_table=11
                     /translation="MQRPWGLSGPEFLQLYWIGLALAVLVAIIVRVRVRAGHSDQPVRS
                     LDMDELAYLAGGPKRVVETSIARLLTAGELRTSRRGTVQVTTTTSSLNPVDRAVITDSQ
                     RYTNRTVGLMIPSVSKDGVVIGIGRRLEEMGLVVHPDVAKSALRKGSIALWVLLAIGVA
                     RWVNGVAIDAPVGWLTLQLVITGALIFLLTRRGKHVRTSKGNRVLNEARTTSSGRAATS
                     DEALYAGAAGLVLFGGLAVYPDLAVRSSLLAASGGGYTGYTGCSAGSSSCSSGGGGGGC
                     GGGCGGGGS"
     gene            complement(8671..9093)
                     /gene="msrB"
                     /locus_tag="C8D87_RS44475"
                     /old_locus_tag="C8D87_11946"
     CDS             complement(8671..9093)
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422371.1"
                     /locus_tag="C8D87_RS44475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D87_11946"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_109642611.1"
                     /transl_table=11
                     /translation="MEPVVGATPKVVRSEEEWRAQLNPAEYAVLRQAGTERPWTGEYTE
                     TKTEGVYECRACGAELFRSDTKFDSHCGWPSFFSPLAGDAVLLREDRTLGMTRVEVLCA
                     TCHSHLGHVFEGEGYPTPTDQRYCINSVSLKLVPSE"
     misc_feature    complement(9092..9108)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 19.68"
                     /tool="antismash"
     gene            complement(9190..9480)
                     /locus_tag="C8D87_RS44480"
                     /old_locus_tag="C8D87_11947"
     CDS             complement(9190..9480)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786714.1"
                     /locus_tag="C8D87_RS44480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D87_11947"
                     /product="hypothetical protein"
                     /protein_id="WP_112232666.1"
                     /transl_table=11
                     /translation="MGDSKEENEKFQEELIGLDPQDPETQAFAEHLDRMKRQRPGYTVE
                     GYLEGVQDFADSANRTTGHQRLVAVIVVGMILLGVILTIWFALSDIITIIF"
     gene            complement(9505..10458)
                     /locus_tag="C8D87_RS44485"
                     /old_locus_tag="C8D87_11948"
     CDS             complement(9505..10458)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099309.1"
                     /locus_tag="C8D87_RS44485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D87_11948"
                     /product="asparaginase"
                     /protein_id="WP_112232668.1"
                     /transl_table=11
                     /translation="MGHEVVAEVWRGDFLESVHHGTVVATDVAGIPALAVGRPGDVAFP
                     RSSNKPLQALAMLRAGLGLDGELLALACASHSGEDFHVEGALRILASAGLGEDALQCTP
                     DLPLGEEARARHLARGLAAAPKYMNCSGKHAAMLATCVANGWSTDDYLDPTHPLQVATR
                     ETIEDVAGEPVAQVGVDGCGAPIFAISLTGLARAFGRLASAPEGTLEHRVAVAMNAYPQ
                     WVGGTGRDVTVLMDALPGVIAKDGAEGVYAVGLPSGEGVALKIADGSSRARAVVVIAVL
                     KRLGVDVSKVEDLATVPVLGHGRAVGAVKPSLALAG"
     misc_feature    10494..10509
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.49"
                     /tool="antismash"
ORIGIN
        1 cggcggcgac gcccgagtac ctggagtgcg acggcgtcgt gctcgcggtc ccggctccgt
       61 cggcgcgcaa actgctgtcc gagatcgcgc cgcaggtgtc cggtgcgtac ggcaaggtcg
      121 aggtcgcctc gatggccgtc gttgcgctgg cactgccgcc gggcaccgaa ctgccggacc
      181 gctccggcat cctgatcgcg gagggggagc ggtacagcga ccgcatcacc ccgttcaccg
      241 ccaaggcgtt cacgttctcc agccgcaagt ggtcgcactt cggcgagccc gtgctggtgc
      301 gcggctcggt cgggcgccgc ggcgagacgg cgctcctgca gcgcagtgac gcggacctcg
      361 tgaaggccgt gcgcaacgac ctgtacgagc tcaccgggat caaggccgag cccatcgacc
      421 acgtggtgca gcgctggggc ggtggcctgc cgcagtacgg cgtcgggcac gtcgacctcg
      481 tcgagaccat cgagaagggc atcggcgacg tacccggcct cgcggtggcc ggcgccacgc
      541 tgcacggcgt cggcgtaccg gcgtgcatcg ccaccgccga cgcggccgcc gcccgtgtcg
      601 cagcacacct gctgggccgc gttcgccgtt gacagggccg tgccaagatg gacgtatggc
      661 gcgcctgaac tacaacgagc tcaacgacac catccgctac acgatgtggt cggtgttccg
      721 cgtcgagccg gggaagctgc cggaagaccg cagcgccgcc gcctccgaga cgcagaacta
      781 cctcgacgcg ttggaaggca agggcgtcgt cgtccgcggt gtctacgacg tggccggtct
      841 gcgtgccgac gcggacttca tgatctggtg gcacgccgag gagatcgagc aggtgcaggc
      901 cgcctacacg ggcttccgcc gcaccccgct cggcaaggtc tccacgccgg tctggtcgca
      961 ggtcgcgctg caccgccccg ccgagttcaa ccgcagccac atccccgcgt tcctcgccaa
     1021 cgaggagccg cgcaagtacg tctgcgtcta cccgttcgtg cgctcctacg agtggtacct
     1081 gctgccggag gaggaccgcc gcaagatgct cgcggaccac ggcaaggaag cccgcgactt
     1141 ccctgacgtg cgcgcgaaca ccgtcgcgtc gttcgcgctg ggcgactacg agtggatgct
     1201 cgctttcgag gccgacgagc tgcaccgcat cgtcgacctg atgcggcacc tgcgcggcac
     1261 cgaggcgcgg ctgcacgtgc gtgaggagat ccccttctac acgggcaccc gcgtcccggc
     1321 cgccgagctg gtcacgaacc tgccgtgacg agaaagggcc gcccctgaac cggcggcggc
     1381 cctctccgtt cagcgcacta gctcaccttg taggcgtgga tcacggtcac ctcgaccgtg
     1441 ttgcccttgg cgtccttcgc cgtggccttc agcgaggtcc acttggcgcc ggccgggtgg
     1501 tagatcaacg cgctgttgcc ggccacaccc gcgttgcgcc acgacttgcc gtcgtcgtgc
     1561 gagtactgga cgccggtgat gcgcaccttg cccgtgtcgc cctgggactg ggtgaccaga
     1621 ccgactcgct ggagcgtgcc cgcgggcacc gcgccgttgt cgtcgagcct cggcgcgaac
     1681 cgcacgaccg agagcgggat ggtgtcggtc acgcccggcg tgcccttgga cctgaacgtc
     1741 cacgccgcgg acaccttggt gctgaactcg agctgcgagc tccggtcgag gctcgcctcc
     1801 gcgcggaagt ctccctccgg gagcggcacg ttctggaagt tgcccccggt gccttcaccg
     1861 atcagcacac cgttgcggta cagcgccgcc cggaaactgt ccacagtgga gccgccctgg
     1921 ccgctgctcg cgtcgctcag catcggcacg ccgatgaaca gctgcgtgcc gttgcgctgg
     1981 gcgtacgggt acggggactc gggcaatgcc ggagagaaca ccggctggtt ctcccgctcc
     2041 tggtaggtct tgcccgcctc gtacgtgcgg aacccggaga cggcctcctc ccggctgccg
     2101 ttcgcgtggg tgatgctcac gatcgactgc cagcgcaggt cctcggtcgt cacgtagtcg
     2161 accgccttgc tgcccgccgg cacctggtag agcgccgaac cgccgcccac gccgtgcacc
     2221 gacgtcggga actcgccctt gagcgcccgg ctgtccggcc tgagaccgcc gatggtggcc
     2281 tcgacgcgtg ccaggtcctt cttgcccggc gcgcgcgtca ggccgttggg caggctgccg
     2341 gcgaccgggt aggacagccg gtagtacacg ttggcctcgg ccgcggagtt cgcgtgcagc
     2401 gtcgccatcg acgccagctc cttcggcgag agcgggccgc cgacctgcgc caccgagatg
     2461 ccgtcgaagc caccgaacgc gatcagccct gcctgcagga ccctgtcgcc gtacgcgcgc
     2521 cggtacgaga gctcaccgac gacgaagtcc ccggtgggtt ccggcaccgt caccgacagc
     2581 ggctgcgcga ggcgggcgtc ctgcttgatg gccaggtcgc cggtcacctt caccgtcgga
     2641 tggaacagca tgtccatgcc gttcggcgag ctgatcgagg agtcgaccac gtacgtgccc
     2701 ttgggcaggc ggatcttctg gtcgccgcca ccgaagacca ccttggacgt cgggatgtcc
     2761 aggccgctga cgaacgtcag cgcgtccacg gggtcctgcc cgtcgcggcc gatcatgtcg
     2821 atcgtgaggg tgtagctctc cacctcgcgc tcgacaccga cgagcgtgcg gacggtgtgc
     2881 gtgccggacg tggcgacgac ctcgccggtg tacgcggcgt cctgcgtgcc gacgcgggtg
     2941 tcgccggtga ccttgacggc cgccgtgccc ttggcgggaa cggtgaccat cgaggcgctc
     3001 agcgagaaca tgccctgcgg cgcaccgctg gtgatcttca cgtcgagcgt gatgtcggcg
     3061 tcggtcgggt tgctgtaggt gatcgacctg gtctccggct tgtcgtcacc gtgcggccac
     3121 tcgtgcaggc cgaggttgac cgcgacgacg tccgcggtca gcacctggtt gatcgccttg
     3181 gccacgtcga tgcgaccggc gccctggtcg aacgggctca cgcccggtgc cggcttggcc
     3241 gtggtggcca gcgccgactt gagctgcgcg ggcgtccact ccgggtgctg ctgcttcagc
     3301 aacgccgcgg caccggccac gtgcggagtc gccatggacg tgccgctcgc cgagacgtgc
     3361 tcggtggagc ccgccttggc ggcgacgatc tcgacgcccg gtgcggtcac ctcgggcttg
     3421 agcccgctgt caccggtgcg agggcccctg ctggagaagt aggcgagctt gtcgtcgcgg
     3481 tcgacggcgc cgaccgacag cgcggcctcg gccgtgctgg gtgagctcac cgtgcccggc
     3541 ccgcccgagt tgcccgccgc gatcacgaac aacgtgccgt acttggcggt gaggtcgttg
     3601 accgcctgct ccagcgggtc gatctccggg gtgtcgcctc ccccgaggct catgttgacg
     3661 acctgggcgc cctgctcggc ggcccagcgc atgccgtcga ggatccacga ctcctggcag
     3721 ccgaactcga cgcagacctt ggcgtcgagc agctgggcgc ccggtgcgac gccgccgtac
     3781 tgcgacccgg cgctcgcgac ggtcgcgccg acgtgcgtgc cgtggccgac cctgtcggtg
     3841 gtgtccgggt cgctggtgaa gttcttctcc gcgatctgct gacccttgag gtccgggtgg
     3901 ttctcgtcca cgccggtgtc gacgatcgcg accttgacgc ccttgccggt gatgccgcgc
     3961 tggtgcgcgg ccggcgcgcc gatctgcgcg acgctgcggt cgaggctcgg cttgcggatg
     4021 ccgtcgagcc agatcttgtc accggaggcg gcctccgccc gcagagcggc ccagtcggcc
     4081 gaggaccgcg ctccaagtct cgcctgaccg tccacgatca acggcatccg gtcccggtag
     4141 ccgaactcca gcagcgtggt gacgtcgaag agtctgcggt cgagccggcc cgccgccagg
     4201 ggcccgaacg cgtccgtcgg cacgaccatc gtgtgcccgt cggcaccgcg gtgggtcttg
     4261 aaggtcatcc ccttgcggcc ctcgccgggc tgaatcgcga cgacctgctg tgcctgtccc
     4321 agaaggacct tgtctccggt gacgagggtg acctcgtcga ccgcccgttg tggaccggtg
     4381 ggttgcggtg cggccgacgc tgcggtggcc gttcccgcgg tcagtgcgag cgccgcgaag
     4441 gcggccccca gcactttcac atttccgcgc attcgaatcc cctcatggtt cgaaatgctg
     4501 cgcgctgtgg tccgcgcagg ccggaaggcc ttctgcgcaa tgtgaaaatg gccccccgac
     4561 cctttcgaac gtatcgacgg tggcgggggt cacacagggc tgaacgggcg attcttgagg
     4621 gagttctgtt cgcccgtcag ttcgactgcc agaacttcca gcgcgccttg cgtttggctg
     4681 acagttctcc tgcggcacgg caccattcgg cgaacgccgc cgcgtcggcg cgggcacgcg
     4741 agcccgcgcg acgagggttt tccagggcgt acgcgtcgaa gagcggccgg aaccggtcgc
     4801 cgagctcgtc ggcgacagcg ggccccagca tccgcacgat gccgcggcgt ttggacagca
     4861 acgcgcggcg ttcgacgtcc agtcgttgtt cgtcgaatcc cgccggagcc ggtccgttcg
     4921 cgagcaatgc atttagcagc tcggcctgtg cggcggcaag cctttcgcgt gcggccgaag
     4981 gatcattccg gtcgatgatt tcagccacgg tgtttgtcca cgacggcgcg gatcgcggcc
     5041 agttcggacg cgatttcggt gtcggacggg tagttgtcgt cgcgttccag cagaacgccg
     5101 ggtggtttca cgcgcgagca cagttcgtcg aggacgccga gcacttccgg cagcaccgcg
     5161 tgggcgtggg tgtcgtggta gacgtcgccg tggtcgacgc cgccggccac gtgcacgtaa
     5221 gccaggcgct ccagcggaat ctcgtcgagg aaccgctccg ggtccgtgcc gatgttgcgc
     5281 gcgttcgcgt acaggttcgc gacgtcgacg agcagccagc agtccgtccg ttcgacgagc
     5341 tcggcgagga actggccctc ggacagctcg ccgtccggcc actccagcag cgcggcgacg
     5401 ttctccagcg cgagcggcac cggcaggttc cgctgcgcca gctcgacgtt cgcgacgagc
     5461 acgtcgagcg cgtccctggt gcgcggcacg ggcatcaggt ggccggagtc gagcccgccc
     5521 gcgcggacga agcagacgtg gtcgctcacc atcggcgcgc cgacggcttc cgcgaccttg
     5581 ccgaggtgct cgacgcgacc gaggtcgacc gggtccgcgc caccgagcga cagcgacacg
     5641 gcgtggggga gcaccggaac tccgcgctcc agcagcagcg tcaccgactc cggcaggtgt
     5701 ccgatgtgga ggttctccgc gaccacctcg acgaagtcca cgccgggcag ccgttccacg
     5761 gtcaggtcga tctccgaacg ccagccgatt ccgacgccca ggtccgcgat gcccgccatc
     5821 agtcgcctcc tcctccgcca ccgccgccgc agctgctgcc accacagctg ctgctgctcg
     5881 acgacgagca gctgctgccg ctgctcgatg aacaactact gctaccacac ccgctgccgc
     5941 agctgctgca actcgacccg ctgccgccac cgtccgaaga ggaccgccgc ttgcggttct
     6001 gcttcagcgg cagcatcctg acgatgctct gggagagctc gaagagatcg gcgaccgggc
     6061 ggcggttcac cctgccgcag aagccgtaga ccgccacggc gtgcacccgg tcggtcgcgg
     6121 tcacccgcag gagggcctcc ctgcccgccc cggtgatggc cttgcgcccg cgctgccaca
     6181 ggaagaacag cacggcgatc gggacgccga gcaggaactc gagcgccacg aagccgagca
     6241 ggaccagcac gaccgcgatc acgagcgaga accaccagcc ctcgttgcgg cgacgcgggc
     6301 gttgcaggag cttccggtcg agcaggtgct ggtgcagcat cgacatctcg gtgctgtgcg
     6361 cggccgaccg cacgatcgtg tcaaaccgga cgggtgtgcg cgcgtagctc aggattcgcg
     6421 cctcgatcgc cgtggtcgcg ccgtggttgt tctggtgcac ggccgagacg aggccgtcgc
     6481 gcgacacccg aacgagaccg gcgtcgatca gccgggcgag cgcggtctcg gcggcacgcc
     6541 cgggacccgc cgtcaagtaa ccgacctgtt ccgcggaaag ctgcgtcccc tgtgttgtca
     6601 ccggtgtgac catgagtttc cccctcaact gctggaactg ctgcagctgc tactactact
     6661 gctaccgcaa ctcgaactgc tgcaactgga actgctgctc gaacagctgc tgctgctgca
     6721 actgctgctg gagcaggaag acgacgacgc gcagttgtgg tgctcgtgaa cgccgccgca
     6781 cccgccactg tccgaatgcg acttctgctt tccgtggtgg tgcaccggtg gcgggtacag
     6841 gattcctggc ggcaagccgt acaggtcggc gaccctccgg cggccgacca caccacgcag
     6901 tccgtgcaac gccacggcat ccacccggct gcgcgggttt cgctgtgcgt gccccaggat
     6961 gcccctgccg atcggcgtga gcatcttctt gtcgcgcagg aggaacgcca cgaaccacgc
     7021 cacgagcgtg acggccagga ggttcaggtc gacgaagagc gccgccgcga ggaacgcgat
     7081 gccgagcagg accagcagga cccgcaacgc caccctgccg ccgctcgtct tgccccactg
     7141 gcggtggacc agcgccttct cgaccagatt gcggtgcagt gcacccatct cctggctgtg
     7201 cgccgccggc cccaccacca ggccgatcgg gcgacccccg ccggacagcc cggcgaggat
     7261 ggacgcctcg agcgggctcg tcgcgccgtg accgttctgg tggacggcgg tcacgagtcc
     7321 ctcacgggag aaccgcacga gaccgccgtc catcagccgg gcgagagcgg cctccgccgc
     7381 cctgcccggt ccccccgcca ggaacccgat ctcctccgcg gcccagttcg cctgcggtgt
     7441 cgaagacttg gccatcagca ctcccctcac tggtgcccgt gcatcttcga cgtgagatct
     7501 acccccgggt tcctggtgtt acctgattca gaccggacag cgggaaatca gctgccgccc
     7561 ccgccgcagc cgcccccgca gccgccacct ccgccaccgc tcgaacacga tgaagaaccc
     7621 gccgaacacc cggtgtagcc ggtgtagccg cccccggaag cggcgagcaa cgaactgcgc
     7681 accgccagat cggggtacac cgccagaccg ccgaacagaa ccagcccggc cgcgcccgcg
     7741 tacagggcct cgtcggacgt ggcggcacgg ccgctgctcg tggtgcgtgc ctcgttcagc
     7801 acgcggttcc ccttggacgt gcggacgtgc ttgccgcgcc gcgtgaggag gaagatgagc
     7861 gcaccggtga tgacgagctg caacgtcagc cagccgacgg gcgcgtcgat cgcgacgccg
     7921 ttgacccagc gggccacacc gatcgcgagc agcacccaca gcgcgatgga tcccttgcgc
     7981 agcgcgctct tcgcgacgtc cgggtggacc accaggccca tttcctcgag cctgcggccg
     8041 atcccgatga ccacgccgtc cttggagacg gaggggatca tcagaccgac ggtgcggttg
     8101 gtgtagcgct ggctgtcggt gatgacggcg cggtccaccg ggttcaagga gctcgtggtg
     8161 gtcgtgacct gcaccgtgcc acgccgtgag gtgcgaagct cacccgcggt gagcagccgc
     8221 gcgatggacg tttcgaccac gcgcttcgga ccgcccgcga gataggcgag ctcgtccatg
     8281 tcgagggagc gcacgggctg gtcgctgtga cccgcccgca cgcgtacccg aacgatgatc
     8341 gcgaccagca ccgccagtgc cagcccgatc cagtagagct gaaggaactc aggtccggac
     8401 aacccccaag gcctctgcat gcgacccacc ccctgactgc cccgaaagtg cttcggtggc
     8461 gtccatggtg tggtccgggc cacagccccg caatgctagt tgactaagcc ttagggttgt
     8521 ccgtcttttg tcaccagttc gtcgctctga ccagctctca gctggacgaa ccgccgtcga
     8581 ctccgctgga gccttcaccg tcggtaccga agaagatcat tgcgcctccc aggtcggccc
     8641 ggcccgatcg accggactcg gctcagtctc ctattcggac ggaacgagtt tcaaggagac
     8701 ggagttgatg cagtaccgct gatccgtcgg cgtgggatac ccctcacctt cgaaaacatg
     8761 cccgaggtgg ctgtggcagg tggcgcagag cacttcgacg cgggtcatgc cgagggtgcg
     8821 gtcctcgcgc aggagcaccg cgtctcccgc cagcggcgag aagaacgacg gccagccgca
     8881 gtgcgagtcg aacttggtgt cgctgcggaa cagctcggct ccgcacgcac ggcactcgta
     8941 gacgccctcg gtcttggtct cggtgtactc gccggtccac gggcgctcgg tgcctgcctg
     9001 gcgcagcacc gcgtactcgg cggggttcag ctgggcgcgc cattcctctt cggaacgcac
     9061 gactttgggg gtggcgccga caacaggttc catgtccctt tataacgcgg gcacggtcct
     9121 cgggcttccc ggcgaccgcc gcgtgcaccc ctgccgtaag accttgttga gaagtcgtct
     9181 tcgtttggct cagaagatga tggtgatgat gtcgctcaac gcgaaccaga tcgtcaggat
     9241 gacgccgagc aggatcatcc cgacgacgat gacggccacc agccgctggt gcccggtcgt
     9301 gcggttcgcc gagtcggcga agtcctgcac gccctccagg tacccctcga cggtgtagcc
     9361 gggtcgctgc cgcttcatcc ggtcgaggtg ctcggcgaac gcctgcgtct cggggtcttg
     9421 ggggtcgagg ccgatcagct cctcctggaa cttctcgttc tcctccttcg aatcgcccat
     9481 aacgctcaag ggtagtgcca ggaatcaacc ggccagggca agtgagggct tgacggctcc
     9541 gacggcgcgt ccgtggccga ggacgggcac cgtggcgagg tcctcgacct tgctcacgtc
     9601 caccccgagc cgcttgagca ccgcgatcac gacgaccgcg cgggcgcgcg acgagccgtc
     9661 ggcgatcttc agggcgacac cctcgccgct cggcaggcca acggcgtaca caccctcggc
     9721 gccgtccttc gcgatgacgc cgggcagggc gtccatcagc accgtgacgt cacggccggt
     9781 gccgccgacc cactgcgggt aggcgttcat cgcgacggcg acgcggtgtt ccagggtgcc
     9841 ttccggggca gaagccaggc ggccgaacgc gcgcgccagg ccggtgaggc tgatggcgaa
     9901 gatcggggca ccgcagccgt cgacgccgac ctgggcgacg ggctcgccgg ccacgtcttc
     9961 gatcgtctcg cgagtggcga cctgcagcgg gtgcgtcgga tccaggtagt cgtccgtcga
    10021 ccagccgttg gcgacgcacg tggcgagcat ggcggcgtgc ttgcccgagc agttcatgta
    10081 cttcggggcg gcggcgagac cgcgggcgag gtgccgggcg cgggcctcct cgccgagcgg
    10141 gaggtcgggg gtgcactgca acgcgtcctc gcccaggccg gccgaggcca ggatccgcag
    10201 cgcgccctcg acgtggaagt cctcgcccga gtgcgaggcg caggccaggg cgagcagctc
    10261 gccgtcgagg ccgaggccgg cgcggagcat cgcgagggcc tggagcggct tgttcgacga
    10321 gcgcgggaac gcgacgtcgc cgggacgtcc gacagccagc gcggggatgc cggcgacgtc
    10381 ggtggcgacg acggtgccgt ggtgcacgga ctccaggaag tcgccccgcc agacctctgc
    10441 cacaacctcg tgtcccatgt cgcccctccc gtgtgcccga gaaactgcca actgtcgaca
    10501 gttgacaacg cggctaatgt aaccaggaga cggcctgtgc cgtccagagc aggagacgaa
    10561 cgtgacacct ccgctgcacc tcagtctggc cgggcaggcc gtggacgtcc tgcgcgagca
    10621 ggtgctcacc ggggagatcc cgccgggctc acgggtcaac gaggtcgagg tggcgcagaa
    10681 gctcgggatc agccgcgggc cgttgcgcga ggcgatccgg cacctggcgt ccgaagggct
    10741 gctcaggctg gtgccgcacc ggggcgcgtt cgtgcccgcg gcggacgccg acgaggtcaa
    10801 ggcgttgttc gagctgcgc
//
