LOCUS       NZ_MCIF01000002        21044 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Thermogemmatispora tikiterensis strain T81 A4R35_scaffold00002,
            whole genome shotgun sequence.
ACCESSION   NZ_MCIF01000002
VERSION     NZ_MCIF01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Thermogemmatispora tikiterensis
  ORGANISM  Thermogemmatispora tikiterensis
            Bacteria; Bacillati; Chloroflexota; Ktedonobacteria;
            Thermogemmatisporales; Thermogemmatisporaceae; Thermogemmatispora.
REFERENCE   1  (bases 1 to 5961682)
  AUTHORS   Tomazini,A., Lal,S., Stott,M., Henrissat,B., Polikarpov,I.,
            Sparling,R. and Levin,D.B.
  TITLE     Analysis of Carbohydrate Active Enzymes in Thermogemmatispora T81
            Reveals Carbohydrate Degradation Ability
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5961682)
  AUTHORS   Tomazini,A., Lal,S., Stott,M., Henrissat,B., Polikarpov,I.,
            Sparling,R. and Levin,D.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2016) Faculty of Agricultural and Food Sciences,
            University of Manitoba, 66 Chancellor's Circle, Winnipeg, Manitoba
            R3T 5V6, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-MAY-2011
            Assembly Method                   :: Newbler v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 25.9x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003268475.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 03:19:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,660
            CDSs (total)                      :: 4,607
            Genes (coding)                    :: 4,541
            CDSs (with protein)               :: 4,541
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 8 of 66
            Pseudo Genes (incomplete)         :: 56 of 66
            Pseudo Genes (internal stop)      :: 6 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            CRISPR Arrays                     :: 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:11:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5573850
            Orig. end                         :: 5594894
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MCIF01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="12"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..21044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21044
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11044](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11044
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    202..218
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 22.43"
                     /tool="antismash"
     misc_feature    226..239
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 18.03"
                     /tool="antismash"
     gene            316..4299
                     /locus_tag="A4R35_RS22255"
                     /old_locus_tag="A4R35_22100"
     CDS             316..4299
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1272: TPR
                     repeat-containing protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF024180.5"
                     /locus_tag="A4R35_RS22255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_22100"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_112433375.1"
                     /transl_table=11
                     /translation="MGETLNILFRTRAEGGFSVEVRDSWSGKSVNGDFIPPFTPRSLTT
                     LLKRLDRQEIDGQGQREIGERLFAALCGRQPGTSGRASARPAYAREQAEAVGGLLHTVI
                     QRTLRRRGTVALTLSFTPGCDEFIPYPWELLHNGDYFLVATGVFTLTRILLRPDEPGGC
                     ELPVHPPLRILYIGAAPPDGEPLETERSYDALRRGLARLVESGQLILDRLEPCTFSELV
                     RYLNAYGGAGILDDSEVQTPCYVVHFDGHGDYGRVCPREECGAFNEPDERRCHACGASL
                     ARVSAQTYLCFCDEQGKRCYIDTHSLRELLVTSDVRLVVLAACETARLEREHSRQRQVA
                     LDSSLATALVTAQIPAVVAMPFSLQDNLSPVFIYHFYEALASGRTLEEALSRARHALLS
                     PHHNGWFIPVLYRHASPGCEEPVPLLAQEEETEEHELALTNFPIPTCFVGREREIKDLT
                     QLITAAVQAEEELSGLRRQQPRSNLRQIVLTGPVGIGKSALAFEVARRNRDKFPGTGGL
                     IGITLQGGKTFAEAVVEMAHAFPGLANRLMLTPDLRQRARHLLNALHARTDRELPCLLL
                     IDGFEEVTDPTESKLWLRFLGELPERVVVLLTSRSHPATLGLQEGAAWRWYEYPIGKMT
                     DQDLLRLFSELAAQSGLDKRIHLDDPVQQEILREICTLLDGYPLGAELIFGAARSIGGK
                     VFTPEAATRSLEEIRDELRETPLAGIWAALEVAYLRLSPAARLLLSYLSAFRLPFSTNQ
                     IIMLVAPHSSERADNPSSAPQVPPAESLAEQGRDVAEGETCSPGTIVPGELAANWRSAR
                     DELVRASFMGFDGRVYSIHPQVRNFALSYLPAEERRRVHRVVAAYYLGLPHLSPEEWFA
                     AFEHLVGAEELGEAVRTAVRASWALCGRGYAFQLLAILRRAGSYAVRLEDKAGEGQIQC
                     CLGAILRQLGNYTEAVACLKRSLQLYQEQNQQEEAAWSFYELAMLACDEGNLSQAADYA
                     EQALASFEQGGNPRGRAWASLVQGVILRRSGRYREALSRSRLALTELKINGDQEGVAWA
                     SLECSRIWLALANYSEALTACEEAQRLFASLGLRTGSAWTLVQQAQLALEHARLDEAEQ
                     ASALALEIFQEQSMRPGEGLALCVRGEVHRALGDWGAARSFYEKALTIFTSLGNRVDQA
                     QVLTALGSLVLAEGALHEAGEYFEQALSLAQEQEARPLTAQALRGLGDTARLLRAFSEA
                     RARYEEALALATELGTSLEISAIHVGLGELAWAQDQRAQAQKHWAQALLLDQRLEHPAR
                     RALQQRLESLLSEDGDQTEALTGTATATLIALEERTDAASEAS"
     gene            complement(4452..6551)
                     /locus_tag="A4R35_RS22260"
                     /old_locus_tag="A4R35_22105"
     CDS             complement(4452..6551)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_4"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013146.5"
                     /locus_tag="A4R35_RS22260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_22105"
                     /product="hypothetical protein"
                     /protein_id="WP_112433377.1"
                     /sec_met_domain="Glycos_transf_4 (E-value: 1.2e-23,
                     bitscore: 82.0, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGLPAIPDAYLVPVILILTFVVSALLSLIFCGIARLLFPKFRSGE
                     HKPGHHRSDLRSDLPASGREIKTIELPLVGGPSFTLAMLITGLVAGYLLFHTGEQWTLL
                     LIGLGATLGFMLIGLVDDWHKVYSKEGLSERAKFSSLFVLSVAAGACYFFLTPKGPESY
                     SPYIGLPIIGHLICPNASPEMSACPLHPGELAYYAWLVFLVLMTGVTGSVTSLAVDFSD
                     GLDGLAGGLVFSAALAFGIVTTGIIDRPEGQVVEVLSLLCAGSTLGYLPWNWPSAWAAR
                     GRRSSAKRLAQIYMGDSGALALGGLLAMIAIFDRQETLLFLVGAAFVLEGLSALSARLI
                     TRFYRRQLHILRFASTREFIPHTEFPRPFLATPLHHHFDLLGWDRRRLVYGAWALGACF
                     AVLGVMVGLAPETWQRYLGRILVLLLAWIVWSSGSWTRRYFVGKHPPERSRRRRLALYY
                     GFPYRIMGIPLYHLVEVIEASEDVIETPAEELALWQRMTIFDARAMLGLYCYRAGYYPA
                     ALAQWTRIPAGNRALRPAIARLLEELDSRLALEKQETQPIKREELLRHQEPPLITGNLG
                     PADVPNPHEPVSGTEAEAVQESWPLPVTKTVEETAERQSTSGGDRLPPEALPRDTRLEA
                     SHNGHSAPDARHLAATTGMGEREPTSTGSSQAARTRGRSHSPLSWLQRFLHRGTRSSHP
                     SQQQL"
     misc_feature    complement(6639..6655)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.88"
                     /tool="antismash"
     gene            complement(6773..8344)
                     /locus_tag="A4R35_RS22265"
                     /old_locus_tag="A4R35_22110"
     CDS             complement(6773..8344)
                     /EC_number="6.3.2.13"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016874 - ligase activity [Evidence IEA]"
                     /GO_process="GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /GO_process="GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /locus_tag="A4R35_RS22265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_22110"
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="WP_112433379.1"
                     /transl_table=11
                     /translation="MILKTLLAALPSPPRAIYPGGSLAETAAVEQLTISQLAYDSRRVE
                     PGTLFIAVPGVHTDGRRFLAEAAARGAVAALGESLEGFAPSPLPLPYIEVAEVRSALAD
                     LAAAFYGYPARQLWTIGVTGTDGKTTTCNLICTILETAGLATGLMTTANFKIKGEEWEN
                     TTRQSTLEALEIQALLRRMLDAGVTHAVIEATSHGLELARVRGCEFDVGVVTNITSEHL
                     DFHGTVENYRRAKARLFEMLDPDRRKALPTRPAAVLNRDDSSFAFLRPFCRAPILDYGI
                     DTPASFQATELELGAAGTRFAVLFPHESQPVPMATRLIGRFNVSNCLAAIAVTYHLGLS
                     PTQIAEGLARARSVNGRMEHITAGQPFSVIVDYAHTPDSLQKVLTTLRPLTPGRLLLVF
                     GSAGERDVQKRPAMGTVAAQMTDFFVITDEDPREEDRLKILREIAEGAIAAGKQEGRDF
                     LCIADRRMAIATALATARPGDTVLLAGKGHEQSIIVGREKLPWNERQVALEELQRLGYD
                     SSTSTL"
     misc_feature    complement(8370..8385)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 19.49"
                     /tool="antismash"
     gene            complement(8396..9406)
                     /locus_tag="A4R35_RS22270"
                     /old_locus_tag="A4R35_22115"
     CDS             complement(8396..9406)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478503.1"
                     /locus_tag="A4R35_RS22270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_22115"
                     /product="queuosine salvage family protein"
                     /protein_id="WP_112433381.1"
                     /transl_table=11
                     /translation="MLPAVEIDPLGVLSSTRGVVEEGEHVWMNSEQIQALSERWPELIG
                     PAGVSASGLAAGYDRYHFFDGSERSVNWILLLDALNFCFWPEKDQPRWGITYQGELLNG
                     YWAEAAALTRAVEEGRPIWDAAYLRDMSREELALIFRSEQEQQIPLFEERLTHVHEVGR
                     VLLERFDGQFSQAVAQAAGSAVRLVQLLVEAFPSFRDVARYRQREVRFFKRAQICVADL
                     HALFRGQNWGAFYDLDQLTIFADYKLPQVLRHFGVLEYHPTLAKRIDQQEPLPAGSEEE
                     IEIRAATVWACELLRQALAARGLPMKAIEIDQQLWLLSQRIPDMRPYHRTRTIYY"
     gene            9724..9924
                     /locus_tag="A4R35_RS22275"
                     /old_locus_tag="A4R35_22120"
     CDS             9724..9924
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A4R35_RS22275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A4R35_22120"
                     /product="hypothetical protein"
                     /protein_id="WP_112433383.1"
                     /transl_table=11
                     /translation="MEEQQLCKLVHRLYHDESFRQACFNDLESVAASERMEPLVLDVLK
                     KLIPHLALGTSLGPPMWWWHP"
     gene            10001..11044
                     /locus_tag="A4R35_RS22280"
                     /old_locus_tag="A4R35_22125"
     CDS             10001..11044
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012568.5"
                     /locus_tag="A4R35_RS22280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_22125"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_189362187.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.2e-20, bitscore:
                     71.7, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSADGEARAREEVFGEARAFLGGRLERLLAEVPAPLREDVALALR
                     QEGKLLAWRAASSAEAQVGRLSEPLAGRWPLLTFLIARSLGPAVALDYAAGAALAVECL
                     VCAIDIFDDLVDDDQTPLLQSVGEARAINAAAALLALAQQAILTVAPGARAPAAEPLAL
                     LGELQAASLACLAGQQRDLLAEAQPLSAYGREDCLALAAAKAGSLMRLACRLGALVAGA
                     SPPLCELFSHLGELLGIAGQLDNDCHDLHASLAPPPALSQPKSDLRRGKKTLPLVLAAS
                     TGCSPEELFALQHGAPVGSALSARLAEAITAAWSICLLYRERARLCLQAIEACQPLPPE
                     LHQLLGL"
     gene            11078..14002
                     /locus_tag="A4R35_RS22285"
                     /old_locus_tag="A4R35_22130"
     CDS             11078..14002
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014567.5"
                     /locus_tag="A4R35_RS22285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_22130"
                     /product="ATP-binding protein"
                     /protein_id="WP_112433387.1"
                     /transl_table=11
                     /translation="MTRTTRSFPRVGRLVAILVLALAYVVLLGSDAWVFFPHSVYLPYA
                     LLQFGVSALAALLFLAVGTLVWLYAYRRGVALLLLCFCACMMVTFLVQTGAAAGDLLLS
                     VMGGVSSALSLLLFTTLLLLFPQNMLAPPPLAASSVQACRSFGRLLRLLLLSLLAPAYL
                     GLVVLLSLLVTCHLAFYDWGTSPRLAWLNFVDYTYYLLTLTGILTSIANLYRRTATLRE
                     QQQRRLFMGAVILAFAPFLLLNVLPWWLHIPLRVDPRFSTLPLALLPPLLGYSILRYQI
                     LVLDRYIRRVVAWIVGLVCLVMLAYAVAVLALILPLPDLPARTLLTVALMAVLAPLVWW
                     LARILTERLFFSELCHYRRLLETPHPLESEGATLDEVAQLMSAAVVNVFETREVCLFVL
                     DDESGYYRLCPALSYAEPGDEARLRLLRWLLPMEPEEALGDDGWLTLTPPLVERLTQAR
                     FPLRLSELQQSFGRSPIGLGRYLQVTVADQELDPLLAPVRAQGRLIGVLVLGERGDHQP
                     YAGPDFEAIELMLARFTPLLETARLSARASRHAAMLNALYSVNALPVSAIETIEDVAVN
                     YAEVAARAGSAGAEVWLYDEQGACLRRVAYLPGGPPLSAAQQLSSLQEDDWRPWFYEGS
                     DMGPEPARDHALSPHIPPCLPQTPSFPFAWLPLRRAHHRLGVLVLTYARAHVFSCEEKH
                     MLEMFSSQCATMLENAKITLELRAAYEQQKELDRLKDQFIMTASHELRTPLTAVLGYIE
                     LLKEYHMHLSPEVREDFIARAHRGCDELVLLVSNIMDASRVHMDAERLKLEAVELRKSV
                     QHVVDILDALSTRERHSLRVHVPVGIYVLADELRLRQVLLNLLSNALKYSPPGTDVDVF
                     CRIEERWVTISVRDYGLGVPREAQKHLFERFVRLDRDMNSPVRGAGLGLYISEQLVQAM
                     GGRIWVESSGVPGEGSTFSFTLQHVTAPRPRREAPRSVRREMAC"
     gene            14266..14541
                     /locus_tag="A4R35_RS22290"
                     /old_locus_tag="A4R35_22135"
     CDS             14266..14541
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013231.5"
                     /locus_tag="A4R35_RS22290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_22135"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_189362189.1"
                     /transl_table=11
                     /translation="MNALRRAAASTITARQREVLTYLAQRDHWSVGEVAALLGVSSAAA
                     TKVLARLERKGLITRTLNALDRRCADITVTRAALALLQAEATDENP"
     gene            complement(14522..15313)
                     /locus_tag="A4R35_RS22295"
                     /old_locus_tag="A4R35_22140"
     CDS             complement(14522..15313)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017604674.1"
                     /locus_tag="A4R35_RS22295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_22140"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_112433391.1"
                     /transl_table=11
                     /translation="MSEQPITSVNTPVTTGSDAAGKRPALPREDFTAGVLAQLDSLYRT
                     ALRMTNNPQEAEDLVQETMLKAFRFAHTYHPGTNLRAWLFRILNTTAINHYRRQSTHPA
                     TTSLPEGEEFYLYNRIKDLAGNEVTAGAEEEVLQRYLDEDVYNALMELPPNFRMPVILA
                     DIEGLSYKEIAEALQIPIGTVMSRISRARRQLQKSLWRYAQERGYLKAARTPNAAEDNG
                     TSTRASRANAADAAAAASDPASAPQSSPESAQADQATDSHP"
     misc_feature    complement(15284..15298)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     gene            16105..16407
                     /gene="rsrA"
                     /locus_tag="A4R35_RS22305"
                     /old_locus_tag="A4R35_22150"
     CDS             16105..16407
                     /GO_function="GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /GO_process="GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rsrA"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /locus_tag="A4R35_RS22305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_22150"
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="WP_112433395.1"
                     /transl_table=11
                     /translation="MNCVEAIARLHLYIDRELSAEEVITVQQHLARCSQCESRFRFDER
                     LRRLIHERCTIERAPARLREAVMRIAQTPPGEPLELDPELEMELRADIALEEFVE"
     misc_feature    complement(16130..16145)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 19.33"
                     /tool="antismash"
     gene            complement(16798..17520)
                     /locus_tag="A4R35_RS22310"
                     /old_locus_tag="A4R35_22155"
     CDS             complement(16798..17520)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014713.5"
                     /locus_tag="A4R35_RS22310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_22155"
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="WP_112433397.1"
                     /transl_table=11
                     /translation="MGSQVSVGIAFLAGLASFLSPCVLPLVPIYLAQLVGESVYQATRG
                     GGSLTARLTTFLHAALFVLGFTCAFVALGATASTLGSFLRANQLLLREIGGVLLVIIGL
                     HLTGLFQIPLLSRQKRFEFRPARPGPLASFVIGLVFAIGWTPCVGLILGSILGLAANAA
                     TLREGVLLLLSYSLGLGLPFLLLGLGLNQVSAALKLLKPHLGKIEVATGIVMALAGLAI
                     FFNLLPALNHYFNWGLSL"
     gene            complement(17687..18952)
                     /locus_tag="A4R35_RS22315"
                     /old_locus_tag="A4R35_22160"
     CDS             complement(17687..18952)
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012298.5"
                     /locus_tag="A4R35_RS22315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_22160"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_189362191.1"
                     /sec_met_domain="Pkinase (E-value: 7.1e-55, bitscore:
                     184.2, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNELAGQSLGGYLLEEEIGRGSMGMVYRGKQFALGRDVAIKILPH
                     ALAKDTSYVARFIREARIIAGLNHPNIVQIYDAGEQKGVLYFVMEYVQGPTLGSLLRLD
                     GQIPQHLAAEYAAQIADALDCAYSERNVIHRDIKPENLMLDRWGKIKVMDFGLARAPGL
                     QRITVARTLVGSIYYASPEQIWGQPLDNRSDIYALGVVLYEMVTGQRPFNGRTLPEVTQ
                     AITSGQLRPPSWLNPQLAPGLEQIILKALARDRTLRYEQARQMAEELRALNLRVEEPRA
                     PTSGGPLSPLPPTNPRAFAVQPPRRPRLAEQRPSFAEWRAQRSATQFPPELPHGRPGPA
                     EPAGLGEWHSSPGYASLLEQLPQQGPAPGRGASETELVPPDDPSEKAGLWQHLQRLLGR
                     SGKGDQEPGPAMPSGDGYTRSG"
     gene            complement(19649..20602)
                     /locus_tag="A4R35_RS22320"
                     /old_locus_tag="A4R35_22165"
     CDS             complement(19649..20602)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909080.1"
                     /locus_tag="A4R35_RS22320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_22165"
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="WP_223258449.1"
                     /transl_table=11
                     /translation="MERKVMFDADKPTEPERELIPEPEPEPEPEPEQDEDADLTSLAQS
                     DSLRLYLREISRIPLLTPAREAELAERAEQGDSDARNQLIMANLRLVVSIAKKYVGQGL
                     SLEDLIGEGNIGLIRAVTKFDYKKGFRFSTYATWWIKQAITRAILEGTRVVRLPVYIME
                     EVMRVKRIMRQLYQEMGREPSPESIGERLGMSPERVNELLIWAEKVFSLDAPLSDEEEN
                     TLGDIIEDARERGPMEVTNQQLLREEIRKVLNQLTTRERQVIELRFGLIDDHDHTLEEV
                     GRKLKVTRERVRQIEERAIRKLRHPQASRYLKDYLD"
ORIGIN
        1 gcgatgggac aggcgaaagg tgggggctaa actgtccccg gcctgtccct gcgtgggggc
       61 ctgaagagca tgagcgagag gcgagaaaac gggtggccgc aacctgcgcc tatccttgcc
      121 aggcgcgggc tggtgcctgc tacagggcgt ctcaggtgca accctggtgc aatcgatttc
      181 gcccctcata tactgctttc tgtgctatac tttaacattg aagcagagaa cttttataat
      241 ggctgaagtc tttctccgaa aaagtccgcg tagcaaagcg cgctgccatt atggaagaga
      301 agagtgaccg cacagatggg tgagacacta aacatactct ttcgcacccg cgctgagggc
      361 ggcttttccg tggaggtccg agatagctgg tccgggaaga gtgtcaatgg cgacttcatt
      421 cccccgttta ctccgcgctc cctcaccacg ctgctcaagc gtctcgatcg tcaagagatt
      481 gatggtcagg gtcaacgcga aattggcgaa cggctctttg cggccctctg tggcaggcaa
      541 cctggtacgt cagggcgggc gagcgctcgc cccgcttatg ctcgtgagca ggcggaagcc
      601 gtcggcggcc tgctgcacac cgtgattcag cgcaccctgc gccgtcgtgg tacggtggcg
      661 ctcacgctga gctttacccc cggttgcgat gaattcattc cttatccctg ggagctgttg
      721 cacaacgggg actatttcct ggtggccact ggcgtcttta cgctgacccg catcctgctg
      781 cgtcccgatg agccgggagg ctgcgaactg cctgtgcatc cacctttgcg catcctctac
      841 atcggggcgg cccctcctga cggcgagccg ctggagaccg aacgcagcta cgatgccttg
      901 cgccgtggtc tggcgcgtct cgtcgagagt ggccaattga ttcttgaccg tctagagccg
      961 tgcaccttta gcgagctggt gcgttatctc aatgcctatg gcggcgccgg catcctggac
     1021 gatagcgaag tgcagactcc ctgctacgtt gtccattttg acggccacgg cgactatggg
     1081 cgagtctgtc cacgcgaaga atgcggagcc ttcaacgagc ctgacgagcg acgctgtcac
     1141 gcctgcgggg cctcgctggc gcgtgtcagc gcccagacct acctctgttt ctgcgatgag
     1201 caagggaagc gctgctacat cgatactcac tctctgcgtg aactgctcgt taccagcgat
     1261 gtgcgtctgg tagtgttggc agcctgcgaa acggcgcgtt tggaacgcga gcactcccgt
     1321 cagcggcagg tagccctgga cagctcgctg gccacggccc ttgtgacggc ccagatcccg
     1381 gcggttgtgg ccatgccctt ctcgctccag gacaacctca gcccggtctt tatctaccat
     1441 ttctacgagg ccctggccag tgggcgcacc cttgaggagg ccctttcccg ggcccgccat
     1501 gcgttgctct ctccccacca caatggctgg tttatccccg tcctctatcg gcatgccagc
     1561 cctggctgtg aggagccagt ccctctgctg gcgcaggagg aagagactga agagcatgag
     1621 ctagccctga ccaacttccc catcccgacc tgctttgtag ggcgcgagcg cgagattaaa
     1681 gatctgacgc agcttattac cgcagcggtc caggccgagg aggagctttc cggtctgcgc
     1741 aggcagcagc cgcgttcgaa tctgcgtcag attgtgctga ctggtccggt cggcatcggc
     1801 aaaagtgccc tggcttttga agtggcgcgg cgtaaccgcg acaaattccc gggcacgggg
     1861 ggtctcattg gcattacgct gcagggagga aagaccttcg ctgaggccgt cgtcgaaatg
     1921 gctcatgcct tccccggtct ggccaatcgc ttgatgttga ccccagatct gcgtcagcgg
     1981 gcgcgccatc tgctcaatgc cttacatgcg cgcactgatc gtgaactgcc ctgcctgctg
     2041 ctcatcgatg gctttgagga agtgaccgat ccgactgagt caaagctctg gctgcgcttc
     2101 cttggcgagc tgcctgagcg ggttgtggta cttttgacct cgcgctctca tccggccacg
     2161 cttggtctgc aggaaggggc ggcctggcgc tggtatgagt atcctatcgg caagatgact
     2221 gaccaggatc tgctccgcct ctttagcgag ctggcggccc agagcggtct agacaagcgc
     2281 attcatctgg acgatccagt ccaacaagag attctgcgcg agatctgtac gctcctggat
     2341 ggctatcctc tgggggcgga gctgatcttc ggcgcagccc gctccatcgg cggcaaagtc
     2401 tttacgcctg aagctgccac ccgctcgctc gaagagatcc gtgatgagct gcgtgagaca
     2461 ccactggcgg gaatctgggc cgccctggag gttgcctatc tgcgtctctc gcctgcggcg
     2521 cgactgctcc tctcgtatct ctcggccttt cgcctcccct tcagcaccaa tcagatcatc
     2581 atgctggtgg ctccccatag ctcagagaga gccgacaatc cctcctcggc cccccaggtc
     2641 ccgcctgccg agtcgttggc cgagcaaggg agggatgtgg ccgaaggaga gacttgcagc
     2701 cccggcacta tcgttcccgg ggagctggcc gccaactggc gatcggctcg cgatgagttg
     2761 gtgcgcgcct catttatggg tttcgatgga cgtgtttaca gcattcaccc gcaggtacgc
     2821 aactttgccc tctcctacct gccagccgag gaacgccgcc gcgtgcatcg ggtggtggcc
     2881 gcctattacc tggggttgcc ccatctcagt cccgaggaat ggtttgccgc ctttgagcac
     2941 ctggtagggg ctgaggagct gggagaagcg gtacgcacag cagtgcgggc ctcctgggcc
     3001 ttgtgtgggc gcggctacgc tttccagttg ctggcgatcc tgcgtcgcgc aggctcctat
     3061 gccgtgcgcc tcgaagataa agcgggcgag ggccagattc agtgttgcct gggagctatt
     3121 ctccgtcagc tcggcaacta tactgaagcc gtggcctgtc tgaagcgcag tctgcagctg
     3181 tatcaggagc aaaatcagca ggaagaggct gcctggtcct tctacgagct ggccatgctg
     3241 gcctgcgacg aaggcaacct tagccaggca gccgactatg cagagcaagc gctggcgagc
     3301 ttcgagcaag gaggcaatcc tagagggcgg gcctgggcct ccctggttca aggcgtaatc
     3361 ctgcgccgct ccggtcgcta ccgggaagcc ctttcccgct ctcgcctggc cctgacagag
     3421 ttgaagatca atggcgacca ggagggagtt gcctgggcct cgctggagtg cagtcgcatc
     3481 tggctggccc tcgccaacta tagcgaggct ctgactgcct gcgaagaagc acaacgcctc
     3541 tttgcctctt tgggactgcg gacagggagc gcctggacgc tggtgcagca ggcccagctc
     3601 gctctggagc atgcgcgtct cgatgaagcc gagcaagcca gcgcactggc cctggagatc
     3661 ttccaggagc aaagtatgcg tccgggggaa ggattggccc tctgcgtccg gggggaagta
     3721 caccgggccc tcggcgattg gggagcggct cgcagctttt acgagaaagc gctgaccatc
     3781 ttcaccagcc tgggcaatcg cgttgaccag gcgcaggttc tcaccgctct gggtagcctg
     3841 gtccttgctg agggcgctct tcacgaagcc ggcgaatatt tcgagcaagc cctctccctg
     3901 gctcaggaac aagaggcgcg tccgctcaca gcccaggcat tgcgtggtct gggcgatacg
     3961 gcgcgcttgt taagggcttt cagcgaggcc cgagcccgct acgaggaggc gctggcactg
     4021 gcgaccgagc taggaacctc gctggaaatc agcgctattc acgtgggact gggtgagctg
     4081 gcctgggctc aggatcagcg cgcccaggcg cagaaacact gggctcaggc cctgctgctg
     4141 gatcagcgcc ttgagcatcc ggcccggcgt gcccttcagc agcgccttga aagcctcctc
     4201 tctgaggacg gagatcagac agaagcgctg actggtacag ccacagcaac cttgatcgcc
     4261 ctcgaagagc gaactgacgc cgcctcggag gcctcctgag caaaagaaga cagaaccagc
     4321 ccatcaagtg cgctcgaaca gcccatcgcc gagatccagc aagagcctgg gcctggggcg
     4381 ccattcctcg gcgatgggta gagccagcct gaatgagcac ggcagaacca gactgcttca
     4441 gtctggttct gctagagctg ttgctgcgaa ggatgactgg agcgtgtgcc gcggtgcaag
     4501 aaacgctgta gccagctcaa gggagagtgc gagcgtcccc gtgttcgcgc agcctggctg
     4561 ctccctgtcg acgtcggttc ccgctctccc atgcctgtcg tcgcggccag gtgtcgtgca
     4621 tctggtgcac tgtggccatt gtgagaggct tccaggcgag tgtcgcgcgg gagtgcctcc
     4681 ggcggcagcc tgtcaccacc cgaggtcgac tggcgctccg cggtctcctc aacggtcttg
     4741 gtcactggca aaggccagga ctcctgcact gcctccgcct ccgtcccact caccggctcg
     4801 tgtggattgg ggacatccgc cgggccaaga ttccccgtaa tcagcggtgg ctcctgatgt
     4861 ctgagcagct cctcccgctt aatcggctgc gtctcctgct tctccagggc caggcgacta
     4921 tccagctcct ccagcaggcg agcaatagct gggcgcaagg cccggttgcc agctggaatc
     4981 ctggtccatt gcgccagggc cgctggataa tagccggcgc ggtagcaata gagaccaagc
     5041 atagcccgag catcgaagat ggtcattcgt tgccagagcg ccaactcctc agccggtgtc
     5101 tcgatgacat cctcactggc ctcgatcacc tccaccaggt gatagagcgg aatacccata
     5161 atgcgatagg ggaagccata gtagagcgcc agacgtcggc ggcgcgaacg ctcggggggg
     5221 tgcttcccaa caaagtagcg ccgggtccac gagccactgg accagacgat ccaggccagg
     5281 agcaggacca gaatacggcc cagatagcgc tgccaggttt cgggggctag accaaccatc
     5341 accccgagga ctgcaaagca agcgcccaag gcccaggccc catagaccag gcggcgccgg
     5401 tcccagccca gcagatcgaa atggtggtgc agcggagtcg ccaggaaagg ccgggggaac
     5461 tctgtatggg gaatgaactc gcgtgtactc gcgaagcgca gtatatgcag ctgtcggcgg
     5521 taaaagcgcg taatcagacg tgccgaaaga gctgacaggc cctccagaac gaaagcggcg
     5581 cccaccagga agagcagtgt ctcctgacga tcgaaaatgg cgatcatcgc cagtaagccg
     5641 cccagagcca gcgctccact atcgcccata tagatctgag ccaggcgttt agcgctgcta
     5701 cgccgtccgc gcgcggccca ggctgacggc cagttccagg gaagatagcc gagtgtactg
     5761 ccggcgcaga gcagcgaaag cacctcgacc acctggccct cgggacggtc aataataccc
     5821 gtggtgacga tgccaaaggc cagcgctgca ctgaagacca ggccgccggc cagcccatcg
     5881 agtccatcgc taaaatccac cgccagcgag gtcacactgc cagtcacgcc cgtcatcagt
     5941 accagaaaca ccagccaggc atagtaagcc agctcgcctg gatgcagagg gcaggcgctc
     6001 atctcggggc tggcgtttgg acagatcaaa tggccgatga tagggagtcc aatatacgga
     6061 ctgtaggact caggcccctt tggcgtcaga aagaaatagc atgcgccggc ggccaccgac
     6121 agcacaaaca agctactgaa cttcgcgcgt tccgacaggc cctccttgct atagaccttg
     6181 tgccagtcat cgaccaggcc gatcagcata aaaccgagcg ttgcgcccag gccgatcagc
     6241 aggagcgtcc actgctcgcc agtatggaag agcagatagc cagcaaccag ccccgtgatc
     6301 aacatggcca gtgtaaacga aggcccgccg accagaggca gctcaatagt tttaatctcg
     6361 cgtccgctgg cgggcaggtc cgagcgcagg tcggaacggt ggtggccagg tttatgctcg
     6421 ccgctgcgga actttggaaa gagcagtctg gcgataccgc agaaaatcag gctgagcagt
     6481 gccgagacca cgaacgtcaa aatcagaata accggcacca gataggcatc aggaatagct
     6541 ggtagtccca tacgatttac tcccccgggg cgaaaagagg cccggacacc atgctggcta
     6601 gtcttggctc aagcgaggcc tgctgatctc tctccacccg catgattttt ctgcagtaat
     6661 tagctctcta cctggtaaaa gaagtagaaa gtaaaaacat accatattca atggactggc
     6721 ccattgatag caaacacaaa cgctcttcgc gactggaaaa ggcgaaaagc ccctagagag
     6781 tcgaggtact gctgtcatag ccaagccgtt gcagctcttc gagagccacc tggcgctcat
     6841 tccagggcaa cttctcgcgg ccaacgataa tgctctgttc atggccctta ccggccagca
     6901 gtaccgtatc gccggggcga gcggtggcca gcgctgtggc gatcgccatg cggcggtcgg
     6961 cgatacacag aaaatcgcgg ccctcctgct ttcctgcagc tattgctcct tcggcgatct
     7021 ctcgcaggat tttcaggcga tcttcttccc gtggatcttc atcagtaata acgaagaaat
     7081 ctgtcatttg ggcggccacg gtgcccatcg ctgggcgctt ttggacatcg cgctcgccgg
     7141 cggagccgaa gaccagcagc aagcggcctg gagtcagggg gcggagcgtt gtcaagacct
     7201 tctgcaggct atcgggcgta tgggcgtagt ccacgatcac agagaagggc tggccagcgg
     7261 tgatatgctc catgcgtccg ttcacgctac gagcgcgggc caggccctcg gcgatctgcg
     7321 tcgggctcag ccccagatgg taggtcaccg cgatagcggc caggcaattg ctcacattaa
     7381 agcggcctat cagacgcgtt gccataggaa caggctggga ctcatgtgga aagagcacag
     7441 caaagcgtgt accggcagcc cccagctcca actccgtcgc ctggaaacta gcgggcgtat
     7501 cgatgccgta gtccaggatt ggggcgcggc agaagggccg caagaaagca aagctactat
     7561 cgtcgcggtt gagcactgct gccgggcggg ttggcagcgc cttccggcga tctggatcga
     7621 gcatctcgaa gaggcgcgcc ttggcccggc gatagttctc gacagtgccg tggaaatcca
     7681 gatgctccga agtaatattg gtcaccacgc ccacatcgaa ctcgcagccg cgtacgcgag
     7741 ccagctccag cccatgggag gtagcctcga tcacagcgtg ggtgacgccg gcatcgagca
     7801 tgcgtcggag cagagcctga atctccaggg cctccagcgt actctggcga gtggtattct
     7861 cccactcttc tcccttgatt ttaaaattgg ccgtcgtcat caagccggtg gccaggccgg
     7921 cggtctccag aattgtacag atgagattac aggttgttgt cttgccatcg gtgccagtga
     7981 cgccgatggt ccagagctgg cgagccggat aaccatagaa ggcggcggcc aaatcggcga
     8041 gagcgctgcg cacctcggcg acctcgatat agggcaaagg cagaggagag ggagcgaagc
     8101 cctccagaga ctcgccgagg gcggccaccg caccgcgagc ggcggcctcg gccagaaagc
     8161 ggcggccatc ggtatggaca ccgggcacag caataaagag cgtgcctggc tcaacgcggc
     8221 gcgagtcata ggccagctga gagatggtca gctgctccac ggcagccgtc tcagctagag
     8281 agccaccggg gtagatagcc cgcggtggcg atggcaaggc ggccaacagc gtcttcagaa
     8341 tcatcttact ccagggataa acagactctg tgtagacctg taattaacgg ttcttctagt
     8401 aatagatagt acgagtccgg tgataaggtc gcatatcggg tatgcgctga ctgagcagcc
     8461 agagctgctg atcgatctcg atcgccttca tgggcagacc gcgggcggcc aatgcctggc
     8521 gaagcagctc gcaggcccag accgtggcgg cccggatttc gatctcttcc tcgctaccgg
     8581 cagggagagg ctcctgctgg tcgatccgct tggccagggt gggatgatac tccagcaccc
     8641 cgaaatggcg cagcacctgg gggagcttgt agtcagcgaa gatcgtcagc tggtcgagat
     8701 cgtagaaggc gccccagttc tggccgcgga agagcgcatg aagatcggcg acgcagatct
     8761 gggcacgctt aaagaagcgc acctcgcgct gacgatagcg ggctacatcg cggaaggatg
     8821 ggaaagcctc gacgagcagc tgcaccagac gcacagcact gcccgcagcc tgagcaacag
     8881 cctgagagaa ttgcccatcg aagcgctcca aaagaacgcg cccgacttcg tgaacgtggg
     8941 tcaggcgctc ctcaaaaagg gggatctgct gctcctgctc gctgcggaag atcagagcca
     9001 gctcctcacg gctcatatca cgtaagtaag cggcatccca tatgggtcga ccctcttcta
     9061 ccgcgcgtgt cagggcggcg gcctctgccc agtagccgtt gaggagctcc ccctggtagg
     9121 tgatacccca gcgcggctga tctttctcgg gccagaagca gaagttgagc gcatcaagca
     9181 agagaatcca gttaacgctg cgctcgctac cgtcgaagaa atggtagcgg tcatagccgg
     9241 ctgccagccc ggaggcgctg actcctgccg ggccgatcag ctccggccaa cgctcgctca
     9301 gagcctggat ctgctcgctg ttcatccaga catgctcacc ttcctctacc acgccgcggg
     9361 tgctgctgag cacccctagt gggtctatct cgacagccgg gagcattgct tggtccccgc
     9421 tttggttcca actgtgcatc ttctttcgct tcgggttgtg gggccctcgc tcaagcgtca
     9481 gattctgggt gcgatctctt cccgattcta gcggcaaggc ccccgttcgt caattgcgtt
     9541 gggaccttat actgccgctt cgcgcctgcc ctccctcctc gcccagttaa tagatacatc
     9601 taaatatata tattctatgc tcttgcattt cttttagata tgtggtatgc tctcacccag
     9661 agcatgcaaa ggcatgctct catgggaagt ctctctagag tgaagagagg gaaggagtgt
     9721 actgtggaag aacagcagct atgtaagctc gtccatcgcc tctatcacga tgaaagtttc
     9781 cgccaggcgt gtttcaacga tctggagagc gttgccgcct ccgagcgtat ggagcctctg
     9841 gtgctggatg tgctcaagaa gctgatcccg catcttgctc ttgggacgtc gctgggtcct
     9901 cccatgtggt ggtggcatcc gtgatgggtg agcgagtgac gggaggagga tgtggaggga
     9961 ggcggggaga gctgtgaggg agcgaggtga gtcgggcaac atgagcgcag acggggaggc
    10021 gagggccagg gaagaggtct ttggggaggc gcgggctttc ctcggcgggc gcctggagcg
    10081 tctgctggct gaggtacctg cgccgttgcg cgaggatgta gctctggctc tgcgtcagga
    10141 gggtaagctg ctcgcctggc gggcggcgtc aagcgctgaa gctcaggtgg ggagactgag
    10201 cgagccgctg gcgggcaggt ggcccttgct gactttcttg attgcgcgtt ctctgggtcc
    10261 tgccgtcgcc ctggattatg ccgctggggc tgctttggcg gtggagtgtt tggtctgcgc
    10321 cattgatatc tttgacgatc tggtcgatga tgatcagaca ccgttgctgc agtctgtggg
    10381 cgaggcacgt gccatcaatg cggctgccgc cttgttggcc ctggctcagc aagccattct
    10441 gacggtcgct cctggggcta gggcacccgc cgccgagccg ctggcgctct tgggagagct
    10501 gcaggcggcg agcctggcct gccttgctgg tcagcagcgc gacctgctcg ctgaggcgca
    10561 gccgctcagc gcttatggtc gtgaggactg tctggcgctg gccgcggcga aggccggctc
    10621 tctcatgcgc ctggcctgcc gtctcggggc cctggttgct ggcgcctctc ctcccctctg
    10681 cgaactgttc tcccatctgg gagagctgct gggaatcgcc ggccagctcg acaacgattg
    10741 tcacgatctg catgcctcgc tggcgccccc cccggcgctc tcccagccaa agtccgatct
    10801 ccgtcgcggg aaaaagacgc tcccgctggt gcttgctgcc tccaccggct gctctcccga
    10861 agagctgttc gccttgcagc acggtgctcc tgtaggtagc gctctctccg ctcgccttgc
    10921 agaggcgatc actgccgcct ggagcatctg cctgctctat cgcgaacgcg ctcgcctctg
    10981 cctgcaggct attgaggcgt gccagcctct tccgcccgaa ttgcatcaac tgctcggatt
    11041 atgattgaga ggatctcagg tgttcaagag ggagtgtatg acgaggacga cgcgctcgtt
    11101 tccgagggtc gggcgtctgg tggcgatttt ggtgctggct ctagcctatg ttgtgctcct
    11161 ggggagtgac gcctgggtct ttttcccgca cagtgtctat ctaccgtacg ctttgctcca
    11221 gttcggtgtc tcagctcttg ctgccctcct ctttctagcg gttgggaccc tggtctggct
    11281 ctatgcttat cgccgtgggg tggcgttgct cttgctctgc ttctgcgcct gcatgatggt
    11341 gactttcctg gtgcaaaccg gcgctgccgc gggtgatctg ctgctctcgg tcatgggagg
    11401 agtcagcagt gccctctcgc tgctcctctt cactactctg cttctcctct tcccacagaa
    11461 tatgctggct cctccgcctt tggcggcctc ctctgtccag gcctgtcgct ccttcgggcg
    11521 tctgttacgt ttgcttttgc tctcgttgct ggctcctgcc tatcttggtc tggtggtcct
    11581 cttgagcttg ctcgtcacct gccatctggc tttctatgat tgggggacca gtccacgcct
    11641 ggcctggctc aattttgttg actacactta ctacctcttg accctgaccg gcattctgac
    11701 ctcgatcgcc aatctctatc gacgcacggc caccctgcgt gagcagcagc agcgccgtct
    11761 ctttatgggg gctgtgatcc tggcttttgc tccctttctg ctcctgaatg ttctgccctg
    11821 gtggttgcat atccccctcc gtgtcgatcc tcgctttagc acccttcccc tggctctcct
    11881 gccgccgcta ctgggctatt cgatcttacg ctatcagatc ctggtgctgg atcgctatat
    11941 tcgccgcgtc gtggcctgga ttgtgggcct ggtctgcctg gtgatgctgg cctatgcggt
    12001 ggcggttttg gctttgattc ttcccctgcc ggatctgccg gcgcgcacgc tgttgacggt
    12061 ggcgctgatg gccgtcctgg cgccgcttgt ctggtggctg gcccgtatcc tgacggagcg
    12121 gctcttcttt agcgagttgt gccattatcg acgcttgctg gagacccctc acccgttgga
    12181 gagcgaaggt gcgaccctcg atgaggtggc gcagttgatg agcgcggcgg tggtcaatgt
    12241 ctttgagacc cgtgaggtct gtctctttgt gctcgatgat gagagcggct actatcgcct
    12301 ctgcccagcc ctttcctacg ccgagccggg cgatgaggcg cgcctgcgcc tgctgcgctg
    12361 gctgttgcca atggagccgg aggaggccct cggcgatgat ggctggctga ccctgactcc
    12421 gccgctggtt gagcgcttga ctcaagctcg cttcccgctg cgtctcagcg agctgcagca
    12481 gtcctttgga cgtagtccca ttggtctggg gcgctacctt caagtcactg ttgctgatca
    12541 ggagctggac cccttgctgg cccctgtgcg ggcccagggg cgcctgatcg gggtcctggt
    12601 gctgggcgag cgcggcgatc atcagcccta tgccggcccc gatttcgaag ccattgagct
    12661 gatgctggct cgctttacgc ctctgttgga gacagcccgc ctctcggcgc gcgccagccg
    12721 ccatgcagct atgctcaatg cgctctatag tgtcaatgcc ttgccagtga gtgccatcga
    12781 aacaatcgag gatgtggcag tcaactatgc agaagtggcc gcgcgcgctg gctcggcggg
    12841 ggctgaggtc tggctctacg atgagcaggg ggcctgtctg cgccgcgtcg cctacctgcc
    12901 cggggggcca ccgctctcag ccgcccagca gcttagctcg cttcaagagg atgactggcg
    12961 cccctggttc tatgagggga gcgatatggg accagagcca gcccgcgacc acgcgctctc
    13021 tcctcatatt cctccctgcc tgcctcaaac gccctccttc cctttcgcct ggctccctct
    13081 cagacgcgct catcaccgtc tcggtgttct ggtcctgacc tatgcccgcg ctcatgtctt
    13141 ctcttgcgag gagaaacata tgctggagat gttctcttcg caatgcgcga caatgctgga
    13201 aaatgccaaa atcactttgg agctgcgcgc tgcttatgag cagcagaagg aactcgatcg
    13261 cctcaaggac cagttcatta tgacggcctc ccatgagctg cgcactcctc tgacggctgt
    13321 gctcggttat atcgaattgc tgaaagagta tcatatgcat ctttcgccag aggtgcgaga
    13381 ggacttcatc gcccgtgctc atcgtggctg cgatgaactg gtgctgttgg tcagcaacat
    13441 tatggatgcg agccgcgtgc atatggatgc ggagcgcctg aagctagagg ccgtcgagct
    13501 gcgcaagtct gtccagcatg tcgttgatat tctggatgcc ctcagcacgc gtgagcggca
    13561 ttccttgcgt gtccatgtgc cagtagggat ctatgtgctg gccgatgagc tgcgcctgcg
    13621 ccaggtcctc ctcaatctgc tcagtaatgc cctcaagtat tctccacccg gcacagatgt
    13681 cgatgtcttt tgtcggatcg aagagcgctg ggtaactatt tcggtgcgcg actatgggct
    13741 gggggtaccg cgcgaggcgc agaagcatct ttttgagcgt tttgtgcgcc ttgatcgcga
    13801 tatgaatagt ccagtgcgcg gcgccgggct gggactctac atcagcgaac agctcgtgca
    13861 ggcgatgggc gggcgaattt gggtggagag tagcggtgtg ccgggcgagg ggagtacctt
    13921 ttctttcact ctgcagcatg tcactgcgcc tcggcccagg cgtgaggcac cgcgcagcgt
    13981 acgtcgcgag atggcctgtt gaactgccac gctgcactga gcgaagggaa agctggctcg
    14041 ggtcaagagg ccggcgcggc cggcggccag ggaacaacag agcaggcagc agcgggggca
    14101 gcgaccgggt tgagcgggag agaggggcag cgtgctaact acaagctgta cctgaatagc
    14161 tccgccgcag cgtctcttat gaggaaagag cttgtgggta ttccccactc gcctgttgcc
    14221 ctgacgtgga gtcttgcttc tactcgtaga gggagcgcta tcctcatgaa cgcactacgg
    14281 cgagcagctg ctagcaccat caccgcgcgt cagcgagagg tgctgaccta tctggctcag
    14341 cgagatcact ggtcagtagg cgaggtagct gccctactgg gggtcagttc ggcagcggct
    14401 accaaagtcc tggcccgtct ggagcgcaag ggcctgatca cgcgcacctt gaatgctctc
    14461 gaccggcgct gcgcagacat cacggttact cgcgcggcgc tcgcgttatt gcaggcggag
    14521 gctacggatg agaatccgta gcctggtctg cttgggctga ctctggactt gactgaggag
    14581 ctgacgctgg gtcggacgct gccgctgctg cgtccgcagc gttggcgcgg ctggctctgg
    14641 tcgaggtgcc gttatcctca gcagcgttgg gtgtcctggc cgctttgaga tagccgcgct
    14701 cctgggcata gcgccagagc gatttctgca gctggcgacg tgcccgtgag atgcggctca
    14761 tgaccgtacc gatagggatc tgcagcgcct cggcgatctc cttgtaagag agtccctcga
    14821 tgtctgccag aatgaccggc attcggaaat taggcggcag ctccatcagc gcattgtaga
    14881 cgtcctcatc aagatagcgc tgaagtacct cctcctcagc cccggcagtg acttcgttgc
    14941 cagccagatc cttgatacga ttgtacaggt aaaactcttc tccctcgggc agcgaggtgg
    15001 tcgccggatg ggttgactgg cggcggtagt ggttgatggc cgtggtgttc aaaatgcgaa
    15061 acagccaggc ccgcaggttg gtgccgggat ggtaggtgtg agcaaagcga aaagctttaa
    15121 gcatggtctc ctgtaccaga tcctcggcct cctggggatt gttggtcatc cgtagcgccg
    15181 tgcgatagag gctgtcaagc tgggccagga cccctgccgt gaagtcctct cgcgggaggg
    15241 ctggcctctt cccagctgca tcgcttccgg tagtcactgg agtattgaca gaagtgatag
    15301 gctgttcaga catgcaaatc tccctctatc ctctctcaac cggacgacca ctgttgcgtt
    15361 gtctcgcggc tgcgatggag tctgcaatag ggacaggggc aggatcagtc cgtaccagcc
    15421 gggccgatcg ctgctgtggc tgtctcctgg gagtctagga ggctgctccg tgcagcggta
    15481 gggcgtatca tcggtgactg tattcattgt ggtctcagct ctgcagaggc tcttttccga
    15541 gcaaaacctg cctttcgcgt acattctcct gcacggaacc gcagtgtgcg gtacctcacc
    15601 agacaatagg ctgtttctag tatagctgat agatcgctgc tcaaactagt cccttctgat
    15661 cgagcaagat ggcgggagcg agtcggctca tggctgactg cgggctaccg atgtttcttt
    15721 actcgataca cagctcaggt cctctttttc gttctctgac ccgctttgcc tggacccgct
    15781 ttgcctggac ccgctttgcc tggctcttcc ttgttcggcg gctggacgct acaatagcag
    15841 ccagctcgct ctcttcaacc cagcattttt ctcggcttcc tggggacaac ataccagaaa
    15901 agaagagact ggtcaaccag gtagctgctg gttgcaaggt ggaacagcct gatatactga
    15961 gcgagcctgt gctcaagtgg gacctgccag gggaaaagcg aggaaacact tccccgggga
    16021 atgaaaatca cctcgccctc tgttaaaatg aacagataag aaaaccagcc tcgatacttg
    16081 aggagagaga gcgagtgaac aaccatgaat tgcgttgaag cgatagctcg tctccatctg
    16141 tatatcgacc gggaactcag cgccgaggaa gttatcacgg tccagcagca tctggctcgc
    16201 tgctcgcagt gtgagagccg ttttcgcttt gatgagcgtc ttagaaggct gattcatgaa
    16261 cgctgtacga ttgagcgcgc ccctgctcgt ctacgcgagg cagtgatgcg cattgctcag
    16321 acgccccccg gtgagccact ggagcttgat cccgagcttg agatggagct gagagcggat
    16381 atcgctctgg aagagtttgt cgagtgagct ggctggctgg ttggttcggt tcgctggcgc
    16441 acaggcgagc cagaccatga tcctcacaga cagtggcagt gccctgttcc cttgtctttc
    16501 ctgctgacct ttttgtctgc ctgcctgtca gtgtttcgat cttatgtctt ctccgcgtgt
    16561 gtgaccagtg ctcgcagtaa ggggcctggc cctgacgcag gaaagcaagc cggcgcggag
    16621 acatccccag tctctgtttc tgtcctgagt gtccagaagg aggcagttgt ctcctcaccg
    16681 gcttcgtttc ttcctcaagc aaggaatgtg gagcgagcca ggcagcttct gtgagccttg
    16741 gtgagagtga ccttgctgtt tccagatgcg gttggcgttc tcccactcag gcgtggctca
    16801 gaggctcagg ccccaattga aatagtggtt gagcgcaggc agcaggttga aaaagatcgc
    16861 cagcccggcc agggccataa cgattcctgt ggccacctcg atcttcccca ggtgtggctt
    16921 gaggagtttg agggctgcac tgacctggtt gaggcccaag ccgagcagga ggaagggtag
    16981 gcccagaccc agcgagtagc tgagcaacaa cagaacgcct tcgcgcagcg tggcggcgtt
    17041 ggcggccagg ccgagaatgc tgccgagaat cagcccgacg cagggagtcc agccgatggc
    17101 gaaaaccagc ccgatcacga acgaggccag cgggccaggg cgagccgggc ggaactcaaa
    17161 gcgtttctgg cgcgagagca gcggaatctg aaacagaccc gtaagatgaa gaccgatgat
    17221 aacgagtaga acgccgccga tctcacgcag caggagctga ttggctcgca ggaaggagcc
    17281 gagcgtgctt gctgtggctc caagagcgac aaaggcacag gtgaagccca gcacgaagag
    17341 cgcagcatgc aggaaggtcg tcaaacgtgc tgttaggcta ccacctccgc gggtcgcttg
    17401 atagacactc tcgcccacta actgcgccag gtagattgga accaggggca ggacgcaagg
    17461 cgagagaaag gatgcaagcc ctgccagaaa agcgatgccc acgctcactt gcgatcccat
    17521 agatgcgtct tctcctcaag caacaaagcg ctggattcgg acagggtccg atgggtgatc
    17581 gtttaacgat cgctcgtagt atagcgaaga ttggccagcc ctgcaagatg acctctgcct
    17641 ggtggcggga aggcaagcct gctggtgcag ccagcccatc tagccgctag ccgctgcggg
    17701 tgtagccatc gccagagggc atcgccggac ctggctcctg atctcctttg cctgagcgcc
    17761 cgagcaaacg ctgaaggtgc tgccagagac cggccttttc tgacggatcg tctgggggca
    17821 ccagctccgt ctcactcgct cctctgcctg gtgctggtcc ctgctggggg agctgttcca
    17881 acagggaggc gtagccagga ctactatgcc attctcccag cccggctggc tctgccggac
    17941 ctggccgacc atgaggaagc tcgggcggaa actgcgtggc tgagcgctgg gcccgccact
    18001 cggcgaacga gggtcgctgc tcggccaggc gcggtcgacg cggcggctgc acggcgaagg
    18061 cccgtggatt ggtcggaggt aaaggggaga gtggtccccc gcttgtgggc gcccgaggct
    18121 cctcgactct cagattcagg gcgcgcagct cctcggccat ctgccgtgcc tgctcgtagc
    18181 gcagcgtccg atcgcgggcc agcgccttca gaataatctg ctccaggccc ggggccagct
    18241 gaggattcag ccaggacggc gggcgcagct ggccgcttgt gatggcctgc gtcacctctg
    18301 gcagggtgcg cccgttgaac ggacgttgcc cggtcaccat ctcgtagaga accacgccga
    18361 gggcataaat atcgctgcgg ttatcgagcg gctgacccca gatctgctct ggggaggcgt
    18421 agtagatact gccgaccagc gtccgggcca ccgtaatgcg ttgcagcccc ggcgcgcgcg
    18481 ccaacccgaa atccattacc ttgatcttcc cccagcggtc cagcatcaag ttttccggct
    18541 tgatgtcgcg atggatcaca ttgcgctcgc tgtaggcgca atcgagcgcg tcggcaatct
    18601 gggccgcata ctcggcggcc agatgctggg ggatttggcc atccaggcgc agcaggctac
    18661 ccagcgtcgg cccctggaca tactccatca caaagtagag cacgcctttt tgctcaccgg
    18721 catcgtagat ctgaacgatg ttggggtggt tcagcccggc gataatgcgg gcctcgcgga
    18781 taaagcgcgc aacgtaagat gtgtctttgg ccagggcatg aggcagaatc ttaatggcga
    18841 catcgcggcc aagcgcgaat tgtttgccgc ggtacaccat ccccatcgaa ccacgcccta
    18901 tttcttcttc caacaggtag ccacccagac tctgtcctgc caattcattc atgtgcgtga
    18961 acgcactggc tccccgcttg tgcgacgctg tgcaaagtag ggagcccagt gccccctcca
    19021 tttctgcgtt cctgcaaagg cccctcatct tctcgctgat tgcctgcctg gtgaccccag
    19081 cttgcaagga ctgatccggc tggcctgctg ccaggccgcc aggtgctctg tcaggagaga
    19141 aggggcctgt ctcatcagcc cagcgagaga aaagcgcttg ccagctagct gacctgacca
    19201 gccgggcgct cttccctgct ttctccataa taacatggca gcggtgccga cgtaattatt
    19261 tacgttcttc gacggggggg catctacaga gaggcgggaa tttgccccaa aagtcatgtc
    19321 ctcactgttt ggttgtgtaa cataagaatt gtttctattc acctatcttt cttcttgtct
    19381 caggggagac ctggcgacga gggagcgagg gcgcagtgag gagggcgcgg aggcaggaca
    19441 ggcagccatc gtgctcatgc agcaggggcg cttggccccc tcctgtcctg ccctggcctg
    19501 ttctgctcgc ggttcgcttc gcggggagaa gggggcgagg ctggccgctg gcggtcggcg
    19561 cggagccgat ctgtctgtcg gagactggcc tcgctgcgag ccttgtcctg acctggggcc
    19621 tgtggcgatc cagccagcgc cgtcgcgctc agtcaaggta gtccttcaag taacggctgg
    19681 cctggggatg gcgcagcttg cggatcgctc gctcctcgat ctggcgcacg cgctcgcgag
    19741 tgactttcag cttgcgaccc acctcttcaa gcgtgtggtc gtggtcgtca atgaggccga
    19801 agcggagctc gatcacctgg cgctcgcggg tcgtgagctg gttcagcacc ttgcgaatct
    19861 cttcacgcag cagctgctgg ttggtgacct ccattggccc ccgctcgcga gcatcctcga
    19921 tgatatcgcc cagcgtgttc tcctcctcgt cggagagagg cgcatccagc gagaagacct
    19981 tttctgccca gatcagaagc tcgttgacgc gctctggact catgccgagc cgctccccga
    20041 tactctcggg tgaaggctcc cttcccatct cctggtagag ctggcgcatg atgcgcttga
    20101 cgcgcatgac ttcctccatg atgtagactg gaaggcgaac cacgcgcgtc ccctccagaa
    20161 ttgctcgcgt aatcgcctgc ttaatccacc aggtggcgta cgttgaaaaa cggaagccct
    20221 tcttgtagtc gaacttggtg acagcgcgaa taaggccgat attgccctcg ccgatcaggt
    20281 cctccaggga gagcccctgg cctacgtact tcttagcgat gctgaccacc agacggaggt
    20341 tggccataat gagctggttg cgcgcatcgc tatctccctg ttcagcccgc tcggccagtt
    20401 cggcctcgcg cgctggggtc agcaggggga tgcgagagat ctcacggagg tagaggcgca
    20461 ggctatcgct ctgcgctagc gaggtcaggt ccgcatcctc atcctgctct ggctccggct
    20521 ccggttccgg ctccggctct ggtatgagtt cgcgttctgg ctcggtcggc ttatcggcgt
    20581 cgaacatcac ttttcgttcc aagtacctgc tcttgtctcc ccacccgggg cagggagcga
    20641 tataatcctc tcttgtggtc ggcctgctgg atcgccgcaa gagtgacaga cagtcccttc
    20701 ctccaggaaa ggaagtaaac gtgccgcaac tctatctact ctgttcttat tgtacgaacc
    20761 ctcaccgcat ctggtgaacc atctgcctgc tccgccttct ttcccaggct accaccttgt
    20821 ccactcgctc ctggagccac gcgctggctc tcttgccccc tcagcccggc cccggagcag
    20881 gctccctttc ttgccagatc ggttgagcat ggcctcattg taccatactg gcacacgatt
    20941 gcaagccgga cccttgcttg cgacctctgt ctatttataa catcgctccg tggagcgtta
    21001 ttctctccag tatagcacga gggccacgca gatgaaatgc cctt
//
