LOCUS       NZ_MCIF01000002        25566 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Thermogemmatispora tikiterensis strain T81 A4R35_scaffold00002,
            whole genome shotgun sequence.
ACCESSION   NZ_MCIF01000002
VERSION     NZ_MCIF01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Thermogemmatispora tikiterensis
  ORGANISM  Thermogemmatispora tikiterensis
            Bacteria; Bacillati; Chloroflexota; Ktedonobacteria;
            Thermogemmatisporales; Thermogemmatisporaceae; Thermogemmatispora.
REFERENCE   1  (bases 1 to 5961682)
  AUTHORS   Tomazini,A., Lal,S., Stott,M., Henrissat,B., Polikarpov,I.,
            Sparling,R. and Levin,D.B.
  TITLE     Analysis of Carbohydrate Active Enzymes in Thermogemmatispora T81
            Reveals Carbohydrate Degradation Ability
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5961682)
  AUTHORS   Tomazini,A., Lal,S., Stott,M., Henrissat,B., Polikarpov,I.,
            Sparling,R. and Levin,D.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2016) Faculty of Agricultural and Food Sciences,
            University of Manitoba, 66 Chancellor's Circle, Winnipeg, Manitoba
            R3T 5V6, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-MAY-2011
            Assembly Method                   :: Newbler v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 25.9x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003268475.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 03:19:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,660
            CDSs (total)                      :: 4,607
            Genes (coding)                    :: 4,541
            CDSs (with protein)               :: 4,541
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 8 of 66
            Pseudo Genes (incomplete)         :: 56 of 66
            Pseudo Genes (internal stop)      :: 6 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            CRISPR Arrays                     :: 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:11:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4538461
            Orig. end                         :: 4564027
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MCIF01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..25566
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="betalactone"
                     /region_number="9"
                     /rules="(HMGL-like and AMP-binding)"
                     /tool="antismash"
     cand_cluster    1..25566
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(HMGL-like and AMP-binding)"
                     /kind="single"
                     /product="betalactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..25566
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:15566](-)"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..15566)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
     gene            complement(423..890)
                     /locus_tag="A4R35_RS24370"
                     /old_locus_tag="A4R35_18095"
     CDS             complement(423..890)
                     /GO_function="GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017999.5"
                     /locus_tag="A4R35_RS24370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18095"
                     /product="CoxG family protein"
                     /protein_id="WP_151728762.1"
                     /transl_table=11
                     /translation="MEITGSQQIKAPRQRVFDALFDPQVLKESIPGCESAEYVENPDGR
                     TGRELKLVISPNVPGLKGPYTVYVRTGEVVAPERVVLLSEPYNALGRIKASCTITLAED
                     GAATTNLSYAAEATLEGKIAATPEIVMKNTLKLALDQFFKNFEKQVGAITA"
     misc_feature    complement(1177..1186)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: medium, score: 16.35"
                     /tool="antismash"
     misc_feature    1259..1272
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 19.91"
                     /tool="antismash"
     misc_feature    1440..1453
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.19"
                     /tool="antismash"
     misc_feature    1441..1454
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.05"
                     /tool="antismash"
     misc_feature    complement(1442..1455)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.07"
                     /tool="antismash"
     gene            1468..3174
                     /locus_tag="A4R35_RS18205"
                     /old_locus_tag="A4R35_18100"
     CDS             1468..3174
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A4R35_RS18205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A4R35_18100"
                     /product="hypothetical protein"
                     /protein_id="WP_112431833.1"
                     /transl_table=11
                     /translation="MIIILLSSLLRFSLILLGWPSLDSDEGTMGLMALHIAYQGAHPLV
                     FYGQDYLAPIDAYLAAFFFLIWGPSVVTLRLSVLLLFCFFLIQIYLLTSLVYNKPLALC
                     TLLIVGAGPGEVVFRELEAAGGSPDVLFFCSCLLLVTCKIVLSRQQRLAVQEQAGLWDG
                     LALGGWGLLAGVSIWSDPLVLPFVGMAFLLLLESYRRQLKLALLLILLAGLLVGLAPEI
                     IYKVTVPPSPTRLALLGPGYHEPAYPPVTASSGAGATGTGMAIQASPMLQIAGALLVSL
                     PLATGGNAICTLGSGQAWPPRGDPQTQLCTVLHGAWSLGYLVLWFLAAGLALKAWWPWR
                     RRDPNPESDERSCALEHARQGARLMVLGGAGGTLFLYCLFPPAALTPWPSSRYLVGLII
                     SIPAVLFPLWCLACSSSQRLRCGLPLPVARLGSMVAVVMAIATWWLGSGQLLAQAPGVQ
                     VLNKEQQALINFLLKRGATRIYTDYWTCDRIAFQSTERIICSVLDAGLQPGLNRYPPYS
                     SLVQAAPALPCYVFPVGSPQDLRLQQRIARGYRYSRLTYMNYAIYEPLLYI"
     misc_feature    complement(1469..1482)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 18.42"
                     /tool="antismash"
     gene            complement(3402..5714)
                     /locus_tag="A4R35_RS18210"
                     /old_locus_tag="A4R35_18105"
     CDS             complement(3402..5714)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019334.5"
                     /locus_tag="A4R35_RS18210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18105"
                     /product="WD40 repeat domain-containing serine/threonine
                     protein kinase"
                     /protein_id="WP_112431835.1"
                     /sec_met_domain="Pkinase (E-value: 2.5e-44, bitscore:
                     149.7, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTLIGSVIGGYTIKQFIGSGGMGTVYLAEDPMIGQQVAIKVVRA
                     EAGDFPEAEKALERFKQEARAVASLDHLHILPLYRYGEEETELGRRAYMVMQYRPEGSL
                     WDWLRRRAGMASQQSLSQVPPLPPGLPSAWPLTLSEAEDYLQQAASALQYAHDRGIVHR
                     DIKPANFLLRIEAGPRVHLLLSDFGLAKFFSFSSATSHIFGTPLYMAPEQFGGEARPES
                     DQYALAVMLYLLLTGRTPFEGDPAQLMHQHLHREPPPLRSFNPALPAELEPVFARALAK
                     SPQERYPSVGAFAAAFAHAAHSRPSPLQPLFGLPTKGEPAGDTETASTMLLREPPDSAL
                     PATRPAAEKIEQLKLPRGGQPLTLGGSPGAAPPSLSLQQRQEAASAPTVFAPSALPATS
                     PDFGQLSPPLLAQPQPLPGTAPGQRVQPPLRPPQPPARPEPGRGGKMSRRSALALMAAG
                     VVGVGAVGTGLYFFLNRRPAQALAVLQGHQDSVTSLAWSPDGTHLVSGSRDQTARLWLA
                     SEARALLTYSQQATPILAVAWSTDGTLLASGGENHSVQVWTTSGALQQTFNDLGAPVSA
                     LTWTERRDVLLAGTLGNGGHELLLSGMTTRSLLKARIRALALAPGGETLAVALDSGLVT
                     TLSLSSRRLLQSYYRHRGAALSVAWSPDGTQLASGGADHQVVIWDSASGVVQERLSHPA
                     AVNGLAWDPTTPGRLATACSDERVRVWTLGSNGSAARATAYSGHHGSVTAVAWSQQGLA
                     SASTDHTIILWRLTGSS"
     gene            complement(6054..6920)
                     /locus_tag="A4R35_RS18215"
                     /old_locus_tag="A4R35_18110"
     CDS             complement(6054..6920)
                     /GO_function="GO:0031267 - small GTPase binding [Evidence
                     IEA]"
                     /GO_process="GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016764.5"
                     /locus_tag="A4R35_RS18215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18110"
                     /product="Yip1 family protein"
                     /protein_id="WP_112431837.1"
                     /transl_table=11
                     /translation="MSWDPNQGGQVPQQPTPPPNPYETPASPTPPPANPYGPPVNPYGQ
                     PPVNPYMPPTYYGGQPGPYPPVMATPLPLEQALRELPRQYMRVISHPSAETFAQELGKA
                     SWDIIWVQLLGLSVLYAIITFFTRLASSALLASILPSAYRALASSASVAGSLLSSLISL
                     ILLVGSFFILQGLIYLLARAFKGQGSFLQQCYSSMLIYGPLLTLFIICSIIGIIPFVSI
                     LGLIVEIIALIYALVLLVFATMGSQRLQGGPASAAVLIPTGGLLLLACCGATILAAMIA
                     ASLNSTY"
     gene            7264..8067
                     /locus_tag="A4R35_RS18220"
                     /old_locus_tag="A4R35_18115"
     CDS             7264..8067
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426295.1"
                     /locus_tag="A4R35_RS18220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18115"
                     /product="COG4280 domain-containing protein"
                     /protein_id="WP_112431840.1"
                     /transl_table=11
                     /translation="MAWAILTAAFLASAVEFVEAFTIVLVVGLTINWRSSLVGTVAAAA
                     TLALLVGALGLALVYWIPLALLRLVIGILLILFGLKWLKKAILRYSGLKPLHDEEAIFE
                     EQMATLRARGEQPRPRLEPFGVALSYKTVLLEGLEVAFIVISFGSSAITPSCSASCALG
                     TAALGASAAGLLVVLVGLLLQAPLKRVPENTLKFLVGIMLTTFGTFWSGEGLGINWPLE
                     DAFLLVLAAFYLLCSALLVLWLKDQARRRVAPGFSSASLPERGGR"
     gene            8067..8264
                     /locus_tag="A4R35_RS18225"
                     /old_locus_tag="A4R35_18120"
     CDS             8067..8264
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A4R35_RS18225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A4R35_18120"
                     /product="hypothetical protein"
                     /protein_id="WP_112431842.1"
                     /transl_table=11
                     /translation="MLPRLLRPLRALYQFLVGDPLILSGVVLLFVLLLLLPVGLAPLHA
                     VLLVVALPVILAASLWRELH"
     gene            complement(8398..9819)
                     /locus_tag="A4R35_RS18230"
                     /old_locus_tag="A4R35_18125"
     CDS             complement(8398..9819)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056264.1"
                     /locus_tag="A4R35_RS18230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18125"
                     /product="selenium-binding family protein"
                     /protein_id="WP_112431844.1"
                     /transl_table=11
                     /translation="MSAGEHHEHAHHPAEHEHHEHHEHNGAACGPGYASPEEATRGPRE
                     KVLYTIALHVGTGVEAPDYLATVDVDPDSPTYSQVIHRTVMPYVGDELHHFGWNACSSC
                     HGDAHKSRRFLVIPGNRSSRIYIVDAQDERAPRLHKVIEPEEIKAKTNLSAPHTVHCLP
                     DGHVMISMLGDGEGNGPGGFLLLDEEFRIAGRWEQKADGMRYNYDFWYQPRHNVMVSSE
                     WGAPKTYFDGFNLEDVQAGKYGRQIHFWDWQRHEIVQSVDLGDKGLIPLEVRFHHNPDS
                     THGFVGAALSSVIWHWERANGSWQVQPVIEVPSVELEGWPFPVPSLITDLLVSMDDRYL
                     YFSNWLHGDIRQYDISDPAHPRLTGQVWCGGLLGKAQPVKGHELVGGPQMLQLSLDGRR
                     LYVTNSLYSSWDNQFYPELARRGSYLLQIDCDPEHGGMQINENFYVDFGQEPAGPARAH
                     EMRYPGGDCTSDIWI"
     misc_feature    9931..9945
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 20.11"
                     /tool="antismash"
     gene            complement(10001..11176)
                     /locus_tag="A4R35_RS18235"
                     /old_locus_tag="A4R35_18130"
     CDS             complement(10001..11176)
                     /EC_number="2.3.3.13"
                     /GO_function="GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271: 2-isopropylmalate synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012885.5"
                     /locus_tag="A4R35_RS18235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18130"
                     /product="2-isopropylmalate synthase"
                     /protein_id="WP_112431846.1"
                     /sec_met_domain="HMGL-like (E-value: 1.1e-84, bitscore:
                     282.2, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTQPQRPQPRIVKFYETTLRDGEQTPGVNYFPEEKLEIARALAD
                     MGFDTLDVGFPVSSPSEFEAVRLIASQVENAEICAIARAVRGDIEAAWEAVKVAAHPVI
                     EPFISVSPIHRQVKLGKSRQEVLEMARTAVAYARTLTDTVDFALEDTTRTEHDFILEVC
                     EAILKEGVRFVTICDTVGYALPWEFGQLIADLKREFPTIRISAHCHNDLGLAVANALEG
                     LRNGAERVDTSFNGLGERAGNAATEEVVMAIRTRSADLGLDVRVDTTKIIPTSRLIAKY
                     SGMQPQWTKAIVGQNAFSHGGGIHQDGVLKDARTYEIMTPESIGLSPTERRIRMGKLSG
                     RAALAAQMRELGYTLEQEQLNRAFEMAKLLLGKKRELEELDLRYIAETAMK"
     misc_feature    11191..11201
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 19.14"
                     /tool="antismash"
     misc_feature    11356..11374
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 20.19"
                     /tool="antismash"
     gene            complement(11532..12530)
                     /locus_tag="A4R35_RS18240"
                     /old_locus_tag="A4R35_18135"
     CDS             complement(11532..12530)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914257.1"
                     /locus_tag="A4R35_RS18240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18135"
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="WP_223258392.1"
                     /sec_met_domain="Peptidase_S9 (E-value: 1.2e-05, bitscore:
                     22.9, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKRTKTIVLIVLALACLMSATLYSLRGPLGLRRSVASPLAIQSH
                     RDEQQEPLTSRRLTPAPTASASLLSPALPGLNYLGFTAHIFSQGGNSLVYYLYVPQPLN
                     PQQRYPLVLLLHGGGERSLPYYTAAQNEQLLLNQSYVRVWSSDYQGPANPHIQQRWPCF
                     VLVPQMKQGQQWVNVPINGGSYHQPAQPSIPLQLTKALLDRVLQAYANNIDRTRLYVTG
                     LSNGGYGTWDAIERWPDLFAAAAPIAGAGDPSLAARIKDLPIWAFHGSADPVVPVSGSR
                     DMIAALRAVGGHPRYTEFAGAGHGVWGYVYSLENSPLRVSDFFPWLFSQHR"
     gene            complement(12457..13947)
                     /locus_tag="A4R35_RS18245"
                     /old_locus_tag="A4R35_18140"
     CDS             complement(12457..13947)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013884.5"
                     /locus_tag="A4R35_RS18245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18140"
                     /product="acyltransferase"
                     /protein_id="WP_112431848.1"
                     /transl_table=11
                     /translation="MSQTSISLSSSSSSARRPSDGHSSSSPAGSGKRPHVYELDPLRAC
                     TALSVIAVHVLAATAFLNGSGVGYAIQNALLVSFHFTREIFMFVTAFALVYVYNGRRFP
                     VGQFWKKRSLGVIFPYVFWSLVYVVVNTHPSSWSTFLKTALFDVATGNASYQLYYILLT
                     IQFYVIFPLFMGLLKRWDRHPWLLLLGSFVLQVLLFYVDYHTVQRSGNAFWHTVAVYQD
                     RFVLMYQFYFVLGGLAALHFEQVRRFVLRHARLIMGLFALTLLALWVHFAQQISVYHES
                     MGYATSVLQPSMVFYSVAVIGLALWLCARWARGLAGLPSQEINKRLSVRLWSLCSDASF
                     GVYLIHVLILNQLLGRLLPLLPGSWPVALRVVLLWCAVAALSLAVSSALMYTPWLSRLV
                     GRAGPRPHARAAGGHQSQREESKAEALPDAGRRQPAPAAKATIALVPAVSQTGPTDTAG
                     VSAPQDLRSGALARYEQAHKNHSLDSAGPGLSDERHAL"
     gene            complement(13950..15566)
                     /locus_tag="A4R35_RS18250"
                     /old_locus_tag="A4R35_18145"
     CDS             complement(13950..15566)
                     /NRPS_PKS="Domain: AMP-binding (31-428). E-value: 3.9e-64.
                     Score: 208.6. Matches aSDomain:
                     nrpspksdomains_A4R35_RS18250_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012711.5"
                     /locus_tag="A4R35_RS18250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18145"
                     /product="AMP-binding protein"
                     /protein_id="WP_112431850.1"
                     /sec_met_domain="AMP-binding (E-value: 1.9e-69, bitscore:
                     232.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTISLPPTRNIGPTPLSRTVLTPGRLLASLQHGARQTPNKVLLI
                     EAESGKELSYSQALAGLALLRQLFGPPPQRIMLALPGSLLSGLIWLTALSSGYDLIPVP
                     PAASADEARRLLARQHPSLLCAADEGESAWSARLLPSSARLFTASQLTALVSHAPEPAR
                     PLPVREGRVYLSTSGSTGEPKWLALSERQITWTAEQVRRSHALTSADRGLTTLPFFHIN
                     APVVSLCASLLAGASLIVAPRFSRRRFWSWVERYEATWISVVPTIVALLLESEECPAFL
                     PGRLRFARSASAPLPPANLRAFEERFGIPLIETYGLSEAGSQVAANPLPPALHKAGSVG
                     RPTGVAMRICLPRGDARSQSQEDEELYDLPPGEVGEICISGPNVIRAYEDGAGASSFQN
                     GWFRTGDLGYFDEDGYLFIKGRLREVINRGGENIAPREIEETLQEHPAVREVAVVGRPD
                     PLYGEVVVAYLSLQEGRQLSVEELRSYARQRLSPPRVPVDYLILPELPRNATGKIDRRL
                     LRTREQARRELQQIAREEVRS"
     aSDomain        complement(14283..15473)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R35_RS18250_AMP-binding.1"
                     /evalue="3.90E-64"
                     /label="A4R35_RS18250_AMP-binding.1"
                     /locus_tag="A4R35_RS18250"
                     /protein_end="428"
                     /protein_start="31"
                     /score="208.6"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="QHGARQTPNKVLLIEAESGKELSYSQALAGLALLRQLFGPPPQRI
                     MLALPGSLLSGLIWLTALSSGYDLIPVPPAASADEARRLLARQHPSLLCAADEGESAWS
                     ARLLPSSARLFTASQLTALVSHAPEPARPLPVREGRVYLSTSGSTGEPKWLALSERQIT
                     WTAEQVRRSHALTSADRGLTTLPFFHINAPVVSLCASLLAGASLIVAPRFSRRRFWSWV
                     ERYEATWISVVPTIVALLLESEECPAFLPGRLRFARSASAPLPPANLRAFEERFGIPLI
                     ETYGLSEAGSQVAANPLPPALHKAGSVGRPTGVAMRICLPRGDARSQSQEDEELYDLPP
                     GEVGEICISGPNVIRAYEDGAGASSFQNGWFRTGDLGYFDEDGYLFIKGRLREVINR"
     CDS_motif       complement(14403..14459)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS18250_0003"
                     /evalue="4.80E-06"
                     /label="NRPS-A_a6"
                     /locus_tag="A4R35_RS18250"
                     /protein_end="388"
                     /protein_start="369"
                     /score="19.0"
                     /tool="antismash"
                     /translation="PPGEVGEICISGPNVIRAY"
     CDS_motif       complement(14526..14564)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS18250_0002"
                     /evalue="4.80E-01"
                     /label="NRPS-A_a3"
                     /locus_tag="A4R35_RS18250"
                     /protein_end="347"
                     /protein_start="334"
                     /score="4.1"
                     /tool="antismash"
                     /translation="KAGSVGRPTGVAM"
     CDS_motif       complement(15003..15047)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS18250_0001"
                     /evalue="2.60E-03"
                     /label="NRPS-A_a3"
                     /locus_tag="A4R35_RS18250"
                     /protein_end="188"
                     /protein_start="173"
                     /score="10.9"
                     /tool="antismash"
                     /translation="LSTSGSTGEPKWLAL"
     misc_feature    complement(15554..15567)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.86"
                     /tool="antismash"
     misc_feature    complement(15721..15735)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.35"
                     /tool="antismash"
     gene            16053..16760
                     /locus_tag="A4R35_RS18255"
                     /old_locus_tag="A4R35_18150"
     CDS             16053..16760
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908627.1"
                     /locus_tag="A4R35_RS18255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18150"
                     /product="response regulator transcription factor"
                     /protein_id="WP_112431852.1"
                     /transl_table=11
                     /translation="MLASNKEHNGSKSKQPSVLIVDDEPQIIDFLQMGFVYEGFQVLVA
                     TSGPEAVRMATQHQPDIIILDIMLPGCDGLEVTRQLRRSHDIPIIMLTARDEVDDRVAG
                     LDCGADDYLTKPFAFRELMARTRAVLRRHGNNVADILSFQDITLDRGTHTVTLRGEPVE
                     LTPREFSLLELFLMHPRQVLSRDTILSRVWGYDYVGDDNIVEVYVRHLREKLHDNPPRL
                     LQTVRGIGYTLRG"
     gene            16764..18356
                     /locus_tag="A4R35_RS18260"
                     /old_locus_tag="A4R35_18155"
     CDS             16764..18356
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014567.5"
                     /locus_tag="A4R35_RS18260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18155"
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="WP_112431854.1"
                     /transl_table=11
                     /translation="MKKRANRLSRLLRHVPIRWRLVLVSLGLLTLLLGSLGVIISLIAE
                     QDLLSNQVDVLCNEAQVAIKGIMKGNQDHPFRLSYTFLPPEKPSTDFLYTASTLAFRLV
                     SPSTNAAILATDGSVLVPGSSSPFALQPIDVPPAQVQQALQRDDQRPPYLVLKGANGQP
                     QLVVFLPLVDTFRDHTLGILQISTSTTPIDDFLTTLHLTLFMGVVVLLGLATALIYPLV
                     SVALHPLVEIERTSQRIAQGELSMRIEPPPTDDEIGRLAHSFNRMVARLEEAFQRQKRF
                     VSDVSHELRTPLTALSGSLEMLLLGADRGDLEAARRLARGMYTEVQRLQRMVEDLLVLT
                     RLDAGRLEMRRELIRADDILATVCEQAEHLAHGQTLRWSVAPGTPALLGDKDRLRQALL
                     NIVDNALKYTPAEGTVELQASPASGGLVMLSVRDTGEGIPPEALPHVFERFFRADPARS
                     RLGRRPGGSGLGLAITRELVEAQGGKIRLDSRLGEGTTVTILFPAAVDGRAAARGEETV
                     RNRSLSQPGSRPS"
     gene            complement(18475..19422)
                     /locus_tag="A4R35_RS18265"
                     /old_locus_tag="A4R35_18160"
     CDS             complement(18475..19422)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555888.1"
                     /locus_tag="A4R35_RS18265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18160"
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="WP_112431856.1"
                     /sec_met_domain="PALP (E-value: 7.7e-67, bitscore: 223.8,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYYSSILETVGNTPLIELKSFSPRPRVRIFAKMEGANPSGSIKDR
                     IALKMIEEAEARGLLKPDSILLEPTSGNTGVALALIATMKGYRFTAVVSEKGTQDKRRM
                     LELYGATVITSPGAAGSNGAIRLAQEIARDDPRYVMLFQYGNEANPAAHYETTGAELLR
                     DLPDVSVVVAGLGSGGTLTGIGRRLKEHNPAIRIIGAEPVQGDSIQGLRNLADGFVPPV
                     LDLRVIDERLLVSSQQAWERTLQLKEQEGIFAGPSSGAVLDVALRVAERMEEGKIVIIL
                     ADGGWKYLSEPRWLVQPAGQSEHTATATLQPSLC"
     gene            complement(19459..20961)
                     /locus_tag="A4R35_RS18270"
                     /old_locus_tag="A4R35_18165"
     CDS             complement(19459..20961)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1180: Decarboxylase, pyridoxal-dependent"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012503.5"
                     /locus_tag="A4R35_RS18270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18165"
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="WP_112431858.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 6e-06, bitscore:
                     23.7, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 7.2e-06, bitscore:
                     23.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGGKGKELDLITMTVTGLRTDTGLAFEPERLRRYGDLVLDELER
                     YLSGIEERPVWQPLPAEIRQQISGQGLPEEGQPFEETLTLLREQILPYPQGNGHPRFAG
                     WINSAPSHASILLKPLAAAMNPNCGIGDHAGQELERCAVRWMMELAGLPTEGSAGVFVS
                     GSSEANFTCLQAARYWAAEQDGWNVRREGLQGHHPPFVLYQSDQGHFSIRRSVEAMGLG
                     SSSIHIVPSTPDYRMDLAALERRIHEDRAAGLRPFCVAATAGTVETGAIDPLQELAELC
                     HAEGLWLHVDGAYGAFGILDERVAPLYRGIDQVDSLATDQHKWLSVPIDCGCALVRHGS
                     ALHDAFRLTQSDDEDDANWMSEYTLQRTRRFRALDAWATVRTAGRAGLARAISNNISMA
                     RLLAHEVAAQPELELISGGPLSIVRFRYAPPTLRDEPTLLNRLNRQLAREIQRRGRVFL
                     TSTRLRGQELLRACLVHYATTAADIQAMVDEVLTVGRALCDL"
     misc_feature    20970..20986
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.28"
                     /tool="antismash"
     misc_feature    complement(21033..21050)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 18.14"
                     /tool="antismash"
     misc_feature    21044..21059
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.16"
                     /tool="antismash"
     misc_feature    21153..21167
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.25"
                     /tool="antismash"
     gene            21214..22473
                     /locus_tag="A4R35_RS18275"
                     /old_locus_tag="A4R35_18170"
     CDS             21214..22473
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016089.5"
                     /locus_tag="A4R35_RS18275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18170"
                     /product="TQO small subunit DoxD"
                     /protein_id="WP_189362011.1"
                     /transl_table=11
                     /translation="MTSGSSSKKRPAYLEPTQKLPAAAQPGSGKSSGRWGFPNVALLPL
                     RLFLGITFLYAGLQKFSDPQFFHPGTPGYIGNQLINFARGSPLHNVLIYVALPHATAVG
                     LLVAVGEIAIGIGTLLGLLLRPAAFGGLLLSLIFFLTASWHVYPYFYGSDIVFVFCWIT
                     LLLVGPLHSGLPSVDGYLLQLFGPEDGHWPRSIFAQVLLIVLGVNNAHELEAWLSPSSA
                     AAVAARPQGQVRRSVQWQHQREVRRSFILGLAAGGLATIVLGLGSSLLFVRSFLAGRQG
                     PVRAPLVRGGASATSSSSGASTATANAGSGAGTSGGTIIARKASLASNSALSFTVPSTG
                     DPGILVHLPSDQFVAYDAVCTHAGCQVEYDPSSQLLICPCHGATYDPAQGARVVSGPAP
                     APLSSVSIRIDSAGDIIALP"
     misc_feature    22833..22853
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 22.49"
                     /tool="antismash"
     gene            22837..24246
                     /gene="leuC"
                     /locus_tag="A4R35_RS18280"
                     /old_locus_tag="A4R35_18175"
     CDS             22837..24246
                     /EC_number="4.2.1.33"
                     /GO_component="GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA]"
                     /GO_function="GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="leuC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484036.1"
                     /locus_tag="A4R35_RS18280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18175"
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="WP_112431862.1"
                     /transl_table=11
                     /translation="MPKSMFEKIWEAHVVRDVPGESTILYIDRHLVHEATSPQAFAGLK
                     AAGRKVRRPDATIAVLDHNVPTLQVRGRRVLELVDQASALCITTMEQHAREFGITLFDM
                     NDERQGIVHIIGPEQGLTLPGMTIVCGDSHTSTHGAVGALAFGIGTSEIEHVLATQCLL
                     QKKPRSMNIRIEGSLGPGITAKDIALYMIGRLGTGVGTGHVVEFSGSAVRSLSMEGRMT
                     LCNMAIEAGARAGMVAPDETTFAYLKGRPFAPKGALFEKAVEAWKQLPSDPGAHFDTVH
                     EISIDGLAPQVTWGTNPGMVVDVTGRVPDPADESDPQKRRAYERALEYMGLEPGQKITD
                     IRVDTVFIGSCTNSRLEDLRLAAQMVKGRHVAPGVRALVVPGSMQVRRMAEEEGLADIF
                     REAGFEWRASGCSMCIAMNGDRLSEGERCASTSNRNFEGRQGKGGRTHLVSPPMAAAAA
                     IAGHFVDIRDF"
     misc_feature    complement(24257..24272)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.51"
                     /tool="antismash"
     gene            24344..24964
                     /gene="leuD"
                     /locus_tag="A4R35_RS18285"
                     /old_locus_tag="A4R35_18180"
     CDS             24344..24964
                     /EC_number="4.2.1.33"
                     /GO_component="GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA]"
                     /GO_function="GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="leuD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032935.1"
                     /locus_tag="A4R35_RS18285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_18180"
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="WP_112431864.1"
                     /transl_table=11
                     /translation="MEPFTRLKGLVAPLNRTNVNTDEITPARFLKTISRTGFAQALFAN
                     WRYLGDDQTPNPDFVLNQPRYQGATILLAGDNFGCGSSREHAPWAIREYGFRCVIAPSF
                     ADIFYNNCFNNGILPVTLPEPTVLELFKEVEATEGYTLEVDLEAQTVTTPDGRVFQFPI
                     DAFRKQAMLQGLDNIGWTLSHLDEIRAFEERRQQEAPWVFSLK"
ORIGIN
        1 atgccattca ggatgccgta gtagcggcca atatcctcag cagtgctctg aagcagggga
       61 gtgtgagcac gaaggatttg gcgcgcgtgc agcgggcgcg cctctggccg acgcggctga
      121 tgcagcgtgt ccaggagcag atccagaggc ggatcattgc ctctagtctg gcgaaggagg
      181 gggagcgctt tcgcgtgccg gcctggttcc gctggctggt gcgacggcgg cttgctcggc
      241 tgctgctggc gcactttatc gggctgggga taccgcgggt cagggtggtg gcctgagcgt
      301 ggcttgagat gattgcaggt gatgcagaga tgtgccccct gcgtagcgcc tcaagaagac
      361 tgacaggcgc tgcgcaggga gcaagtggat aggagttgtt tttcgcacgc gtgcgcactc
      421 agtcaggccg tgatggcgcc gacctgcttc tcgaagttct tgaagaactg gtcgagggcc
      481 agcttgaggg tatttttcat cacgatctcg ggagtggcgg cgatcttgcc ttcgagagtg
      541 gcctcggcgg cgtagctcag gttggtggtg gcggcgccat cctcggcgag cgtgatagtg
      601 cagctggcct tgatgcggcc cagggcgtta tatggctcgc tcagcagtac aacgcgctcg
      661 ggggccacaa cctcgccggt gcgcacgtaa acggtataag gccccttcag accggggacg
      721 ttgggagaaa taaccagctt cagctcacgt cccgtgcgcc catccgggtt ctcaacgtac
      781 tcggcgctct cgcagccggg aatgctctct ttgagcacct gtggatcaaa cagggcgtca
      841 aagacgcgct ggcgcggggc cttgatctgc tgactgcccg tgatttccat cgcggtctcg
      901 aaaccttcct cgcaagaaag tctggccctg ctcagcccac acggcgtgat agaggcaccg
      961 ggggctggca gggaggtgtc aggaatcttc ctcaacacga tcagtatatc ccctgtatga
     1021 agtcgcttca atccagaggc taggaagtac cacaagagga ccgggacacg agtggatgat
     1081 tctggtcgct caggctgacc agctttcggg tggttagaga ttggagcaag ccgagcaggg
     1141 agtattcgcc ctgcatgatg atcgcggcgc ctctgctcaa ataacatcac tggaaccgct
     1201 cagactggtg gagctgaaaa atgactggtt ctatgcatgt ctctaataca tactcatata
     1261 taaatattgg aaagtataag agggtccttc agtagttgcg ttccttgcag ataaggagtg
     1321 aactcagaga ttttgaggtg tggattggcg tttatctatt tagattgctc tggctactcc
     1381 tcttccccct ccagtaaggg ctgtagtaac atcggaggca cagcattgaa ggtatggaga
     1441 atttttgata aatatgatgt atttgcgttg attattattc tactttcttc tctgctgcgt
     1501 ttttcgctga tcctcctggg ctggccatct cttgatagcg acgagggaac aatggggctg
     1561 atggctctgc acattgccta tcagggtgct cacccgcttg tcttctacgg tcaggactac
     1621 ttagctccca tcgatgccta tctggcggcc ttctttttcc tcatctgggg tccctcagtt
     1681 gtcaccctgc gcctgagtgt attgcttctc ttttgctttt ttctgataca gatctattta
     1741 ttgactagtc tggtctataa taaaccgctg gcgctctgca ctttgctcat cgtgggggca
     1801 ggtcctggcg aggtagtctt tcgggagctt gaggctgctg gaggatcacc ggatgtcctc
     1861 ttcttctgct cctgtctgct cctggtcact tgcaagatcg tcttgagcag gcagcagcgg
     1921 ctcgcggtgc aggagcaggc tgggctgtgg gatgggctgg ctttgggagg ttggggcctg
     1981 ttggctggcg tcagcatctg gagtgatccg ctcgtgctcc ctttcgttgg catggctttc
     2041 ttattgctgc tagagagcta tcgccgccag ctcaagctag ccctgctctt gatccttctg
     2101 gcgggcctgc tggtgggcct ggcgccggaa atcatctaca aagtgacggt tccgccttcg
     2161 ccgaccaggt tagctctgct cggtccgggc tatcatgagc ctgcctatcc tcccgttacg
     2221 gcgagcagtg gagcgggagc gacagggacc gggatggcga tccaggcttc gccaatgctg
     2281 cagattgctg gagcgttgct cgtcagcctg cccttagcga cgggaggcaa cgccatctgt
     2341 accctggggt ctggccaggc ctggccacct cggggagacc ctcagacgca gttatgcacg
     2401 gtgctgcatg gtgcctggtc tcttggctac cttgttctct ggtttctggc agcggggctg
     2461 gccttgaaag cctggtggcc ctggcgacga cgagatccca accctgagtc cgacgagagg
     2521 tcctgtgcgc ttgagcatgc cagacaggga gccaggttga tggtgctcgg gggagctggt
     2581 ggcacccttt ttctctattg cctgttccct ccagcggcgc ttaccccctg gccttcctct
     2641 cgctacctgg taggactgat catcagcatc ccggcggtgc tcttccccct ctggtgtctt
     2701 gcctgctcgt cctcgcaacg cctgcgctgc ggacttcctt tgccggtggc tcgattggga
     2761 tcaatggtag cagtggtgat ggcgatcgct acctggtggc tggggagtgg tcagctcctg
     2821 gcccaggccc ctggggttca ggtgttgaat aaggagcagc aggctttaat caactttctt
     2881 ctgaaaaggg gcgctacacg tatctatacc gactattgga cctgtgaccg gatagccttt
     2941 cagagcacgg aacgcattat atgcagtgtg ctcgatgccg ggttgcagcc tggtctgaat
     3001 cgttatccgc cctatagctc cctggtccag gcggcccctg ctcttccatg ctatgtcttc
     3061 cctgtcggtt ctcctcaaga tctaagactt cagcagcgca tagcacgcgg ctatcgttat
     3121 agccgtctga cctacatgaa ttatgccatc tatgagccat tgctatatat atagctacgc
     3181 agtgttttgc ttcatctaga gatctggatt atccaacgat gccaggctca aaggctccgc
     3241 tcctggatag caggactgga tctgtctctc actcatggcc gggtggggcc tggggaagga
     3301 cggtgaaggc ggccaagcca accaggcccc agagcctgac cagagcgatt ctgcgcttac
     3361 ctcgcttccc cagctgatgg actggcctga gcagctggca gctaggaaga gccagtgagg
     3421 cgccagagaa tgatggtgtg atctgttgaa gcggaggcca gcccctgctg gctccaggcc
     3481 accgcggtaa cgctgccgtg atggccgcta taggccgttg cccgggctgc agagccgtta
     3541 ctacctaatg tccaaacccg cacgcgttcg tcgctgcacg ctgtcgccag gcggcctggc
     3601 gttgtcgggt cccaggccag cccattgacc gccgctggat ggctcagacg ctcttgaacg
     3661 acgcccgagg cgctgtccca gatcaccacc tggtgatctg ctccgcccga ggccagctgc
     3721 gtcccgtcag gggaccaggc cacgctcaag gcggcgccgc ggtgacggta gtaactttgc
     3781 aacaggcgtc ggctgctcag gctcagcgtc gtcaccaggc cgctatcgag cgccacagcc
     3841 agcgtttcgc cgccgggagc cagggccaaa gcccgaatgc gggccttcag caggctgcgc
     3901 gttgtcatgc cgctcaaaag cagctcatgg ccgccattgc cgagcgtgcc agccagcaac
     3961 acatctcgtc tctcggtcca ggtcagggcg ctaacaggtg cccccagatc attgaaggtc
     4021 tgctgcaagg ccccgctggt tgtccagacc tgcactgagt gattctctcc ccctgaagcc
     4081 aggagggttc cgtcggtgct ccaggccact gccagaatag gggtggcttg ctggctgtag
     4141 gtcagcaggg cacgggcctc gctcgccagc cagaggcgcg ctgtctgatc gcgtgagcct
     4201 gagaccaggt gcgtcccatc gggagaccag gccaggctgg tgacgctatc ttgatgccct
     4261 tgcagcacgg ccagagcctg ggccgggcgc ctgtttagga agaagtagag gccggtgccc
     4321 acagcaccca cgccgaccac tcctgctgcc atcaaggcca gggcgcttcg ccggctcatc
     4381 ttccctccac ggcctggctc tggccgggcc ggtggctgtg gggggcgcag ggggggctgg
     4441 accctctggc ctggagctgt ccctggcaac ggctggggct gtgccagcag gggcggactg
     4501 agctgcccaa agtctggaga agtggctggc aaagctgaag gagcgaaaac ggtcggggca
     4561 gaggccgctt cctgacgttg ctgcaggctc agagatgggg gtgctgctcc tggagagcca
     4621 ccgagagtca gcggctggcc gccgcgcggc aacttcaact gctcaatctt ctccgctgcg
     4681 ggcctggtgg cgggcaaggc ggaatctggc ggctcccgca gcagcatggt gctcgctgtc
     4741 tcggtgtcgc ctgctggctc ccccttcgtt ggcagcccaa agagtggctg caatggcgac
     4801 gggcgactat gagcagcatg ggcgaaggcc gcagcaaaag ctccaaccga aggatagcgc
     4861 tcctgtggac ttttagccag agcgcgggca aagaccggct ccaactctgc cggcagcgcc
     4921 gggttgaagg agcgcagcgg aggcggctcc cgatgtagat gctgatgcat caactgcgcg
     4981 ggatcgcctt cgaagggcgt gcgcccggtc agcagcaaat agagcatgac cgccagggcg
     5041 tactgatcgc tttcggggcg cgcctcgccg ccgaactgct caggcgccat atagagaggc
     5101 gtcccaaaaa tgtggctggt agctgagcta aaagaaaaaa acttggccag accgaaatct
     5161 gagagcagca gatgcacgcg cggcccggcc tcgatgcgca acaagaaatt ggctggtttg
     5221 atatcgcggt ggacaatgcc gcgatcgtga gcgtactgca gggcggaagc tgcttgctga
     5281 aggtagtctt cggcctcgct cagagtgaga ggccaggccg agggcagacc tgggggcaaa
     5341 ggaggaacct ggctgagcga ctgctgggag gccatacccg cgcggcggcg cagccagtcc
     5401 cagagcgagc cttccgggcg atactgcatg accatatagg cgcgccgacc cagctcggtc
     5461 tcttcctctc catagcgata gagcggcaga atatgcaaat gatccaggct tgctacggcg
     5521 cgcgcttcct gtttaaagcg ctccagtgct ttctcggcct cggggaagtc gccggcctcg
     5581 gcgcgtacca ccttaatggc gacctgctga ccaatcatgg ggtcctcggc caggtaaacg
     5641 gtgcccatac cgccgctgcc gataaactgc ttgatggtgt atcccccgat cactgagccg
     5701 atcagtgtat ccactgcctt tgtctctcct gctcgtcaag cgttcgctta gcgggactgc
     5761 atctcatcca gggagcgggc agagcgcgcg taagtaacag gttggccagc caggagacgg
     5821 tccacccgcg agggaaccca tctctgactt actcacttgc cgatatgctg gctctgccca
     5881 cagcagaaca gcccatcctg gcataatagc atgagccggg ctgcctgtcg accgctgctg
     5941 gcccgatcgg ggacctggtc taccgatctt gagcgattct agagaaacga tctggtcacg
     6001 ctcgtcactc tctcctcctc ttcggctggc cttcctaccc gcctgccact tagttagtag
     6061 gtggagttaa gtgaggcggc aatcatggct gccaggatgg tggcgccgca gcaggccagc
     6121 agcaagagac cgccagtggg gatcagcacc gcggcgcttg ccggaccgcc ctgcaggcgc
     6181 tggctgccca tcgtggcaaa gacgagcaga acaagggcat agatgagggc gatgatctcc
     6241 acaatcaagc cgagaatgct cacgaagggg atgatcccga tgatgctgca gatgatgaag
     6301 agagttagca gtggcccgta gatgagcatg ctgctgtagc attgctgcag aaaactgccc
     6361 tgtcccttga aggccctggc cagcaaatag atcagccctt gaagaataaa gaagctgccg
     6421 accagcaaga taagggaaat gagcgaagag agaagagagc cagcgacgct ggccgaactc
     6481 gccagtgctc tgtaggcgct gggtaagatg ctcgccagca aggcactgga ggccagcctg
     6541 gtaaagaagg taatgatggc atacagtact gagagtccca gcagctgtac ccaaatgata
     6601 tcccaggaag ccttccccag ctcttgggca aaggtctctg ccgacggatg cgagatcacg
     6661 cgcatgtact gacgtggtag ctcgcgcagg gcctgctcca acggtagcgg cgtggccatg
     6721 accggtggat aggggccggg ctgcccgcca tagtaggtcg gtggcatata ggggttcacc
     6781 ggcggctgcc catagggatt gactggcggc ccatagggat tagcgggtgg tggtgtgggt
     6841 gaggcaggtg tctcataggg gttaggtggc ggtgtaggct gctgtggcac ttgccctccc
     6901 tggttggggt cccaactcat cgatgtaagc tcctttccag cttctcacaa gagatcaatc
     6961 aatccatcag atgaatggat acaggcttct caatgataac ataacgttat aggttgcgtc
     7021 tactacaatt gctcatccct ttgcttgcca gagcagtcgt attatgccta taatcaggga
     7081 gaaaaacttg ctcagcggcg cgtccgtctc ttcgtctcct cttctcatgt gcgcccctgt
     7141 cgcttcgctc agcgcggggc tatcccgtgt gatcggtggc tgcctgtgag gcggctctgc
     7201 cttgccagag cgcgttaggg gtggctctca tccttgttta gacagaaagg ggaacagctc
     7261 tctatggcct gggcgattct gactgctgcc ttcctggcct cggcggtcga atttgtcgaa
     7321 gccttcacca ttgtgctcgt tgtgggattg actatcaact ggcgcagctc gctggtcgga
     7381 actgtcgccg ccgctgccac gctggccttg ctggtggggg ccctgggttt ggctctcgtc
     7441 tactggattc cgctggccct gctgcgtctc gtgatcggca tcctgcttat tctctttggc
     7501 ctcaaatggc tcaagaaggc tattctacgc tatagtggcc tcaagccgct gcacgacgag
     7561 gaggctatct ttgaggaaca gatggcgacc ctgcgggcgc gcggcgagca gccacgcccg
     7621 cgtctggagc cgtttggcgt ggctctctcc tacaagaccg tcttgctaga ggggctggag
     7681 gtggctttta tcgtgatctc ctttggcagt agcgccatca ctcccagctg cagcgccagc
     7741 tgcgcccttg gcacggcggc cctcggcgcc agcgctgccg ggctgttggt ggtgctcgtt
     7801 ggcctgctgc tgcaggcccc gctcaagcgt gtcccggaga atacgctcaa gtttctggtc
     7861 ggcatcatgc tgacgacctt cggcaccttc tggagcggcg agggtttggg tatcaactgg
     7921 ccgctagagg atgcttttct gctggtgctg gccgccttct atttgctttg ctccgccctg
     7981 ctggtgctct ggctcaagga ccaggcccgt cggcgtgtcg ctccgggctt cagctccgcc
     8041 agtctgcctg agagaggagg ccgctgatgc tgccgcgact gctcaggccg ctgcgggcct
     8101 tgtaccagtt tctcgtgggc gatccgctca ttctcagtgg ggtggttctg ctcttcgtcc
     8161 tgcttttgct gctgccggtg ggcctggccc cactccatgc ggtgctcctg gtagtggccc
     8221 tacccgtgat tctggccgcc tcgctctggc gtgagctgca ctagcgccct gagagaggcg
     8281 tgacaaagtc tagcgaggcg gacctgcgtg gagtggagtt cctttccttg tcccacccgg
     8341 gtccgcctcg tgacagagga agggagtgct gcaaggacag tgaccgcctt gggcgagcta
     8401 gatccagatg tctgaggtgc agtcgccgcc gggatagcgc atctcatggg cgcgcgctgg
     8461 ccccgctggc tcctgaccga agtcgacgta gaagttctcg ttgatctgca tgccgccgtg
     8521 ctcgggatcg cagtcgatct gcagcaggta ggagccacgc ctggccaact cgggatagaa
     8581 ttgattgtcc caactgctgt agagcgagtt tgtcacgtag agccgccgtc cgtccaggct
     8641 cagctgcagc atctgcggtc cgccgaccag ctcgtggcct ttgaccggct gggccttgcc
     8701 gagcaggccg ccgcaccaga cctggccagt caggcgtgga tgggccggat cgctgatgtc
     8761 gtactggcgg atatcgccgt gcagccagtt cgagaaatac aggtagcggt cgtccattga
     8821 gaccagcagg tcggtgatga gcgaggggac cgggaagggc cagccctcca gttcgaccga
     8881 gggaacctcg atcaccggct gcacttgcca gctcccattg gcgcgctccc agtgccagat
     8941 gacgctgctg agggcggcgc cgacaaagcc atgcgtgctg tcgggattgt ggtggaagcg
     9001 cacctccagc gggatcagcc ctttgtcgcc caggtcgacg ctctgaacga tctcatgacg
     9061 ctgccagtcc cagaaatgga tctggcgccc gtatttgccg gcctggacat cctctagatt
     9121 gaaaccgtcg aagtaggtct tcggcgcccc ccactcgctg ctgaccatga cgttgtgccg
     9181 cggctgatac cagaagtcgt agttgtagcg cataccgtcg gccttctgct cccagcgccc
     9241 ggcgatgcga aactcctcgt cgaggagcag aaagccgcca gggccgttac cctcgccgtc
     9301 gcccagcatc gaaatcataa cgtgtccgtc gggcaaacag tgcacggtat gaggggcgct
     9361 caggttggtc ttggccttga tctcctccgg ctcaatgact ttgtgcaacc tgggggcccg
     9421 ctcgtcctga gcgtcgacga tgtagatacg gctggagcga ttgcccggga tgaccaggaa
     9481 gcgccgcgac ttgtgggcgt cgccgtggca ggagctacag gcgttccagc cgaagtgatg
     9541 cagctcatcg ccgacgtagg gcatgacggt gcgatgaatc acctgcgaat aggtgggcga
     9601 gtcgggatca acgtcaacgg tggccaggta gtcgggagct tcaacgccgg tgccaacgtg
     9661 cagggcaatg gtatagagca ccttctcgcg cggccctcgg gtagcctctt caggcgaggc
     9721 ataacccggc ccacaggcag caccgttgtg ctcatggtgc tcatgatgct catgctctgc
     9781 tggatggtgg gcatgctcgt gatgttctcc agcactcatg attctctcct ccggcttgac
     9841 ctcttccagg ccggcgggcc gggcgccctg gctggctgat caagctcagg cccgtcggcg
     9901 ttcgttccta caacccatta catgatttag tttatccaga aaatcaacaa tatgcaagag
     9961 gagaaaagcg ccgccaggac aggcggcgcc gtagcgagca ctacttcatt gctgtttcgg
    10021 cgatgtagcg caggtccagc tcctccagtt cgcgcttctt cccgagcagc agcttggcca
    10081 tctcgaaggc gcgattgagc tgctcctgtt cgagggtata gcccagctcg cgcatctggg
    10141 cggcgagggc ggcgcggccc gagagcttcc ccatgcgaat gcggcgctcg gtggggctca
    10201 ggccgattga ctcaggggtc atgatctcgt aggtgcgggc atccttgagc accccatcct
    10261 gatggatgcc gccgccgtgg ctgaaggcgt tctgacccac gatggccttg gtccactggg
    10321 gctgcatgcc cgagtatttg gcgatcaggc ggctggtagg aataatctta gtggtatcga
    10381 cgcgcacatc caggcccagg tctgccgagc gtgtgcggat ggccatcacc acctcctcgg
    10441 tagcggcatt gccggcgcgc tctcccaggc cgttgaaaga ggtatcgacg cgctccgcgc
    10501 cgttgcgcag gccctccagg gcgttggcca cggccagacc gagatcgttg tggcagtgag
    10561 ccgagatgcg gatcgtcgga aactcgcgct tgaggtcagc gatcagctgg ccgaactccc
    10621 agggcagggc gtagcccacg gtgtcgcaga tggtgacgaa gcgcacgccc tccttgagga
    10681 tggcttcgca gacttccaga atgaaatcgt gctcggtgcg cgtcgtatct tcgagggcga
    10741 aatcgacggt gtcggtcagg gtgcgggcgt aggccacggc ggtgcgggcc atctccagca
    10801 cttcctggcg gctcttgccc agcttgacct ggcgatggat gggggagacg ctgatgaatg
    10861 gctcgatcac cggatgggcg gccactttga cggcctccca ggcggcctca atgtcgcccc
    10921 tgacggcgcg ggcaatggcg cagatctcag cgttctcgac ctgcgaggcg atcaggcgca
    10981 cggcctcgaa ttcgctcggt gaggagaccg gaaagccgac atccagggta tcgaagccca
    11041 tgtcggccaa agcccgggcg atctctagct tctcttcggg gaagtaattg acgccggggg
    11101 tctgttcgcc atcgcgcagg gtggtttcat agaatttgac gatgcgtggt tgcggtcgct
    11161 gtggctgggt cgtcattgtt ctctccttac tgcaatgtaa cacactgcct gcgcgccctt
    11221 gcgcagagca gcgagtgaac acgcgagaag caagacagga gacgctcgct caactccgcc
    11281 tgcgcccatg tggacccacc cttgccctcc cttcctcgct cttcctcagc ttctgctatt
    11341 tgccggtctc cctgtcatct gtagacatta tatgcctctc cttgaagggg aggcaagcgc
    11401 cgctctgtcc agcgggaacc gggccgggca gagcggcggc gtctcgtctc gccccttacc
    11461 catagatact cgtctcgccc cttacccata gatacctgag aagcggctgc ctgcctacct
    11521 gcctcctccg ctcagcggtg ctgcgagaag agccagggaa agaagtcgct gacacgcagg
    11581 gggctatttt ccagcgagta gacgtagccc cagacgccgt gtccggcccc ggcgaactcg
    11641 gtatagcgag gatggccgcc aacggcgcgc agggccgcga tcatgtcgcg cgagccgctg
    11701 acgggcacca ctggatcggc ggagccgtgg aaggcccaga tcggcaggtc cttgatgcgc
    11761 gcggccagcg agggatcgcc ggcgccggcg atgggggcgg cagcggcaaa gaggtcaggc
    11821 cagcgctcaa tggcgtccca ggtgccgtag ccgccgttcg agaggcccgt gacatagagg
    11881 cgcgtgcgat cgatgttgtt agcgtacgcc tggagtactc gatcgagcag ggctttggtg
    11941 agctggagcg ggatacttgg ctgagctggc tgatgatagg agccgccgtt gatggggacg
    12001 ttgacccatt gctgcccctg tttcatttgt ggaaccagga cgaagcaggg ccagcgctgc
    12061 tggatgtgtg ggttggccgg cccctggtag tcgctgctcc agacacgcac gtaggactga
    12121 ttgagcagaa gttgctcatt ctgagcagcg gtatagtagg gtagactgcg ctcgcctccg
    12181 ccgtggagga gtaggaccag cgggtaccgt tgctggggat tgagcggctg gggcacatac
    12241 aggtagtaga ccaggctgtt gcccccttgc gagaagatgt gcgcagtgaa gcccaggtag
    12301 ttgaggccag gcagggctgg ggagagcaga gaggcgctcg ctgtaggagc cggcgtcagg
    12361 cgccgactcg tcagcggctc ctgctgctca tcgcgatgag actggattgc cagcggtgag
    12421 gcaacgctgc ggcgcaggcc gagggggccg cgcaggctat agagcgtggc gctcatcaga
    12481 caggccaggg ccagcactat caagactatg gtttttgtgc gcttgctcat agcgggccag
    12541 tgctccagac cggagatctt gaggcgcgga aacgccagcg gtatctgtgg ggccggtctg
    12601 cgagactgcc ggcacgaggg cgatggttgc ttttgctgct ggagccggct gccttctgcc
    12661 ggcgtcgggc agggcctcgg ctttgctctc ctccctctga ctctgatggc cgccagcggc
    12721 gcgggcgtga ggccgcggcc ccgcacgccc taccagacgg ctgagccagg gtgtgtacat
    12781 aagcgcacta ctgaccgcca gcgagagcgc ggcaacggca caccagagca ggaccacacg
    12841 cagggcaaca ggccagctgc ctggtagcag gggtagcaag cggccaagca gttggttgag
    12901 aatcaagaca tggatcaggt aaacaccgaa ggaggcgtcc gagcagaggc tccagaggcg
    12961 cacgctcagg cgcttgttga tctcctgact gggcagacct gccagtcctc ttgcccaacg
    13021 ggcgcaaagc caaagggcca ggccgataac cgctacgcta tagaatacca tacttggctg
    13081 caagacagaa gtagcgtagc ccattgactc gtgatagacg ctgatctgct gggcaaagtg
    13141 gacccagagg gccagcaaag tcagggcgaa gaggcccatg atcaggcgcg cgtgccgcaa
    13201 gacaaaacga cgtacctgct cgaaatggag ggccgccagt ccacccagca caaagtagaa
    13261 ctgatacatc agaacgaagc gatcttgata gacggccacc gtgtgccaga aagcgttgcc
    13321 gctgcgctgc acggtatggt agtcgacgta gaagagcagg acctgcagca cgaagctgcc
    13381 gaggagcagg agccagggat ggcgatccca gcgtttgagc aggcccatga agagcgggaa
    13441 gatcacgtag aactggatgg tgagcagaat atagtagagc tgataggagg cgttgcccgt
    13501 cgccacatcg aagagggccg tcttgagaaa ggtactccag gagctggggt gcgtgttaac
    13561 gacgacgtag accagactcc agaagacata aggaaagatg acccccaggc tgcgcttctt
    13621 ccagaactgc ccaactggaa aacggcgccc gttgtagacg tagacgaggg cgaaggccgt
    13681 gacgaacatg aagatctcgc gcgtgaagtg gaatgagacc agcagggcgt tctggatggc
    13741 gtagcccacg ccgctcccat taaggaaggc agtggctgcc aggacatgca ccgcgatgac
    13801 actgagggcg gtgcaggcgc gcaagggatc aagctcatag acatgcggtc gcttgccgct
    13861 accggctggc gagctggagc tatgaccgtc gctggggcga cgagcgctac tgctgctact
    13921 gctgaggctg atactggtct gactcaaagc taactcctga cctcctccct ggcgatctgc
    13981 tgtagctcgc gccgcgcttg ctcacgggtg cgcagcaggc ggcggtcgat cttgccagtg
    14041 gcattgcgcg gcaactcggg caggatcagg tagtcgacgg ggacgcgcgg tggactgagg
    14101 cgctggcggg catagctgcg cagctcctcg acgctgagct ggcggccttc ttgcaggctc
    14161 aggtatgcca cgacgacctc tccgtagagc gggtcgggtc gtccaacgac ggcgacctcg
    14221 cgcaccgccg ggtgttcctg cagggtctcc tcgatctcgc gtggggcgat gttctcgccg
    14281 ccgcggttga tgacctcgcg cagccgcccc ttgatgaaga gatagccgtc ctcgtcgaag
    14341 tagcccagat caccggtgcg gaaccagccg ttctggaaag aggaagcacc ggcgccatcc
    14401 tcgtaggcgc ggatgacgtt ggggccggag atgcagatct cgcccacctc gccaggcggt
    14461 agatcgtaca gctcttcgtc ttcctgactc tggctgcgag cgtcgccgcg cggcaggcag
    14521 atgcgcatgg ccacaccggt cggacggccc accgagccgg ccttatgcag cgcgggcggc
    14581 aagggattcg ccgcgacctg gctgccggcc tccgacagtc cgtaggtctc gatcagagga
    14641 atgccaaagc gctcttcgaa ggcacgcagg ttggccggcg gcagcggggc cgaggcgctg
    14701 cgcgcgaagc gcagccggcc tggcagaaag gccgggcact cctcgctttc gagcagcaag
    14761 gccacaatgg tgggcacgac gctgatccag gtggcttcgt agcgctcgac ccaggaccag
    14821 aagcgtcgcc ggctgaagcg cggggccacg atgagcgagg cgccggccag cagcgaggca
    14881 cagaggctga cgacgggcgc gttgatatgg aagaatggca gggtggtcag gccgcgatcg
    14941 gcgctggtca gggcgtgact gcggcgcacc tgctcggcgg tccaggtgat ctggcgctcg
    15001 ctgagggcca gccacttcgg ctctcctgtg ctgccggagg tactcaggta gacgcgtccc
    15061 tcgcgtaccg gcagaggccg agctggctct ggagcatggc tgacgagggc ggtgagttgg
    15121 gaggccgtga agagacgtgc gctggagggc aggagccggg cgctccaggc gctctctcct
    15181 tcatcggcgg cacagagcag gctcggatgc tgacgcgcca gcaggcgccg ggcctcgtca
    15241 gcgctggccg ccggtggaac ggggatcagg tcatagccgc tgctgagggc tgtcagccag
    15301 atcaaaccgc tgagcagact gcccggcagg gcgagcatga tgcgctgggg tggcggaccg
    15361 aagagctgac gcagaagggc cagtcccgcc agcgcctggc tgtagctcag ctctttgccg
    15421 ctctcggcct cgatgagtag gaccttgttc ggcgtctgac gggcgccgtg ctgcaggctg
    15481 gccaggagac gaccaggcgt gagtacagtc cgcgagaggg gcgttggccc gatatttctc
    15541 gttggtggta ggctgatagt agtcatagac gtatccgttc ctgagagaga ttatctctgc
    15601 gttgctgcaa tcagacagac aacctgcaag cgaatcacgt tgctttgctt tgcctgagat
    15661 agggcagctg gattgacacc atagtgagca cagcttttga gtagcacaag cattgtcttt
    15721 tgaaactgcc attcatcttg ccacctgggg ctgaaaaaag gccgcagcga gcctgaaaat
    15781 tcgctgaaat ggcgtcgctg gcagctctac aagcgctctc gtcgctgagg ctggccgtaa
    15841 gaaaaatggg cggcgcaccc gctgcagggg gatgaaagga agggaaaagg ggggattcag
    15901 cgaattttca gccgggcttg ctatatacca tgcaggtatt gttcactatc cctgggggct
    15961 ggcaggtttg ccacttcagg cccccctgag cgtataggaa gaagagagcg tctgctttgg
    16021 cacttgtata agatagggca caggatttca gcatgctggc gagcaataag gagcataacg
    16081 gtagcaagag caagcaacca tcggtcctga ttgtcgatga tgagccgcag atcatagact
    16141 ttctccagat ggggtttgtc tacgaaggct ttcaggtgct ggtggccacc agtgggccgg
    16201 aggcggtgcg catggccacg cagcatcagc cggatatcat cattctggat atcatgctgc
    16261 ccggctgcga tggcctggag gtcacgcgcc agctacggcg cagtcacgat atcccgatta
    16321 tcatgctcac ggcccgcgac gaggttgacg accgcgtggc cgggctggac tgcggcgccg
    16381 acgactacct gaccaagccc ttcgcctttc gcgagctgat ggcgcgcacg cgggccgtgc
    16441 tgcgccgtca cggcaacaac gtcgccgaca tactctcgtt ccaggacatt acgcttgacc
    16501 ggggcactca taccgtcacc ctgcgcggtg agccagtcga gctgacgccg cgcgagttca
    16561 gcctgctaga gctgttcctg atgcatccgc gtcaggtcct ctcgcgcgac accattctct
    16621 cgcgcgtctg gggctatgac tacgtcggag acgacaacat tgtcgaggtt tacgtccgcc
    16681 atctgcgcga gaagctgcac gacaatccgc cacgcttgct gcaaacggtg cggggtattg
    16741 gctataccct gcgaggctaa agcatgaaga agcgcgccaa tcgtctgtcg cgactcttac
    16801 gccacgtgcc gatccgctgg cgcctggtgc tggtctctct tggcctcttg accctcctgc
    16861 tcggctcgct cggagtgatc atctccctga ttgccgaaca agacctgctc tctaatcagg
    16921 tcgacgtcct ctgcaacgag gcccaggtgg ccatcaaagg catcatgaaa ggcaaccagg
    16981 atcacccttt ccgcctctcg tacacctttt tgccgccgga aaagccttcg acggactttc
    17041 tctataccgc ctcgacgctg gcttttcgcc tcgtgagtcc cagcaccaat gccgccattt
    17101 tggccaccga cggtagtgta ctggtgccgg gcagcagctc cccctttgcc ttgcaaccta
    17161 tcgatgtgcc gcctgcgcag gtgcaacagg ccctgcagcg cgatgaccag cgccccccct
    17221 atctggtgct caagggggcc aatgggcagc cgcagctggt cgtcttcctg cccctggtcg
    17281 ataccttccg cgatcacacg ctgggcattc tccagatcag cacctcgacg acgccgatcg
    17341 atgatttcct gacaacgctg catctgacgc tcttcatggg ggtggtggtc ctgctgggcc
    17401 tggccacggc cctgatctat ccgttggtga gcgtagctct gcatccgctg gtcgagatcg
    17461 agcgtaccag ccagcgcatt gctcagggcg agctgtcgat gcgtatcgag ccaccgccaa
    17521 cggatgatga gatcgggcgt ctggctcact cctttaatcg catggtggcg cggctggagg
    17581 aggctttcca gcgccagaag cgctttgttt cagatgtctc ccatgagctg cgcacgccgc
    17641 tgacggctct cagcgggagt ctggagatgt tgctactggg ggccgatcgt ggtgacctgg
    17701 aggcggcgcg acgcctggcg cgtggcatgt atactgaggt gcagcgtcta cagcgcatgg
    17761 tggaagattt gctggtgctg acgcgcctcg atgcgggtcg cctggagatg cggcgtgagt
    17821 tgatcagagc cgatgacatt ctggcaacgg tctgtgagca ggcggagcac ctggcgcatg
    17881 gccagacgct gcgctggagc gtagccccgg gaacaccggc cctgctgggc gataaggacc
    17941 gtctgcgcca ggctctgctc aatattgtcg ataatgcctt gaaatataca cctgccgagg
    18001 gaacggtcga gctgcaggcc agtccggcca gcggtggtct ggtcatgctc agcgtgcgcg
    18061 atacgggcga ggggattccg cccgaggccc tgccacatgt ctttgaacgt ttcttccgcg
    18121 ccgacccggc ccgcagccgg ctggggaggc ggcccggtgg cagcggcctg gggctggcca
    18181 tcactcgtga gctggtggag gcccagggcg gcaagatccg cctcgatagc cggctgggcg
    18241 aagggaccac ggtcacgatt ctcttccccg ccgctgttga tggacgcgcc gcggcgcgcg
    18301 gtgaggagac ggtgcgcaat cgcagcctgt cgcagcctgg cagccgcccg tcgtgagacc
    18361 aggtcggtcg gtcggctctg ctcgtgctcg tcgaagagac aggggggcgg gtgagggaga
    18421 gcagcgggaa aaagaaaagg gagggagggg gacgccaggg gagggcgtcc cgccttagca
    18481 gagtgaaggt tgcagggtag ctgtggcagt gtgctcgctc tgccccgctg gctgcacgag
    18541 ccagcgcggc tcgctcaggt acttccagcc gccgtcggcc aggatgatca caatcttgcc
    18601 ttcttccatg cgctccgcca cgcgcagggc gacgtccaac acggccccgc tcgacggacc
    18661 ggcaaagatc ccttcttgct ccttgagctg cagcgtgcgc tcccaggctt gttgactgga
    18721 gaccagcagg cgctcgtcga tgacgcgcag atccagcacc ggggggacga agccatcggc
    18781 caggttgcgc aaaccctgga tgctgtcacc ctgaactggt tcggccccaa taatgcggat
    18841 ggccgggtta tgctccttga ggcggcggcc gatgccagtc agggtgccgc cggagccgag
    18901 gccggccacc accacgctga cgtcgggcag gtcacgcagc agctccgccc cggttgtctc
    18961 gtaatgggcg gcgggattgg cttcgttccc atattggaaa agcatcacat agcgcggatc
    19021 atctcgcgcg atctcctgag ccaggcgaat ggcgccgttg ctgcccgccg cgcccggcga
    19081 ggtgatcacc gtggccccgt agagttccag catgcgccgc ttatcctgtg tccccttctc
    19141 cgagaccacc gctgtgaagc ggtagccttt catggtggcg atcagggcca gggccacgcc
    19201 cgtgttgccg ctggttggct ccagcagaat ggaatcgggc ttgagcaggc cacgggcctc
    19261 ggcctcctcg atcatcttga gggcgatgcg atccttgatg ctccccgagg gattggcccc
    19321 ttccatcttg gcgaagatgc gcacgcgcgg acgcgggctg aaactcttca gctcgattaa
    19381 gggtgtatta cccactgtct ctagaatgct gctgtaatac atggtagaag atattccctc
    19441 ctttcaaggg cgcccggctc agagatcgca gagcgccctg ccgacggtca gcacctcgtc
    19501 gaccattgcc tggatgtcgg cggcggttgt ggcgtagtgc accaggcagg cgcgcagcag
    19561 ctcctggccg cgcaggcgtg tgctggtcag aaagacgcgt ccgcgacgct ggatctcgcg
    19621 cgccagttgc cgattgaggc ggttgagcag cgtcggctcg tcgcgcaggg tgggcggcgc
    19681 gtagcggaag cgtacgatgc tcaggggacc gcctgagatc agctcaagct cgggctgggc
    19741 cgcgacctca tgggcgagca ggcgggccat actgatattg ttgctgatgg cgcgggccag
    19801 cccggcgcgg cctgctgtgc gcaccgtggc ccaggcatcg agggcgcgga aacggcgcgt
    19861 gcgctgtaag gtatattcag acatccagtt ggcatcgtcc tcgtcgtcgc tctgggtcag
    19921 gcggaaagcg tcgtggaggg cggagccgtg gcgcacaagg gcgcagccgc agtcgatggg
    19981 gaccgagagc cacttatgct ggtcggtggc cagtgagtca acctggtcga tgccgcggta
    20041 aagcggagcg acccgctcgt cgaggatgcc gaaggcgccg taggctccgt cgacgtgcag
    20101 ccagaggccc tcggcgtggc agagttcggc cagctcctgc aggggatcga tggcgcccgt
    20161 ctcgacggtg ccagcggtgg ccgccacaca gaaaggacgc agcccggcgg cccggtcctc
    20221 gtggatacgg cgctccaggg ccgccagatc catgcggtag tcaggggtcg agggcacgat
    20281 gtgaatcgag gagctgccca ggcccattgc ttcgaccgag cggcgaatgc tgaagtggcc
    20341 ctggtcggac tggtagagca caaatggtgg atggtggcct tgcaggccct cgcggcgcac
    20401 gttccagccg tcttgttcgg cggcccagta gcgcgcggcc tgcagacagg taaagttggc
    20461 ctcggagctg ccgctgacga agacgccggc gctgccttcc gtcgggaggc cggccagctc
    20521 catcatccag cgcacggcgc agcgctccag ctcctggccg gcgtggtcgc cgatgccaca
    20581 gttggggttc atagctgccg ccagcggttt gagcagaatg ctggcatggg agggggccga
    20641 gttgatccag ccggcgaagc gcggatggcc gttgccctgg ggatagggca gaatctgctc
    20701 gcgcagcagt gtcagggtct cctcgaaggg ctgtccctcc tcgggcaggc cctgcccact
    20761 gatctgctgg cgtatttcag caggcagagg ctgccagaca gggcgctcct cgataccgct
    20821 gaggtagcgc tctaactcgt cgagaaccag atcgccgtag cgacgcagac gctctggctc
    20881 aaaggcaaga ccggtatcgg tccgcaagcc tgtcacagtc atagtgatga gatctaactc
    20941 ctttcctttc cctccaagca tgccactgct gtctgaaatt tgggcacagg cttgctaaaa
    21001 attcgctgaa tctgagccgt ccaggagggt agttcacaga aactttacat ttttttgtca
    21061 gttgcttgat ctctcctcag taaggtaggg gcgaaagcga tccgcacgga aggcagctgg
    21121 ctggcgcggt caggcggaga gcggagagca atggtgcttg tcggccagcc agccaggaag
    21181 atgtttttaa gcctgcaagg gtggaaggag accatgacat caggttcgtc ttcgaagaag
    21241 cggcccgcct atctggaacc gacccagaag ctgccagccg cggctcaacc cggaagcggc
    21301 aaaagcagcg ggcgatgggg ctttccgaat gtggcgctgc tgcccctgcg cctctttctg
    21361 gggatcacct ttctctatgc gggcctgcag aagttcagcg atccacagtt ttttcatccc
    21421 ggtacccctg gctacatcgg taaccagctt atcaactttg cccgcggctc gcctctccat
    21481 aatgtcctga tttatgtcgc gctaccacat gccacggcgg tcggcttgct ggtggccgtg
    21541 ggagagatcg ccattggcat tggcacgctg ctcggcttgc tgctgcgccc cgcggcgttc
    21601 ggtggcttgt tgctcagcct gatcttcttc ttgacggcaa gctggcatgt ctatccctat
    21661 ttttatggat cggacatcgt gttcgtcttt tgctggatta cgctgctcct ggtcgggcca
    21721 ctacacagcg ggctgccgag cgtcgatggc tatctgctgc agctctttgg gcctgaagat
    21781 ggacactggc cccgatccat ctttgcccag gtgcttctga tcgtgctggg agtcaacaac
    21841 gcacacgagc tagaggcctg gctctccccg tcttcggcgg ctgcggtagc ggctcgccca
    21901 cagggccagg tgcggcggtc tgttcagtgg cagcatcagc gcgaggtccg gcgcagtttt
    21961 atcctgggcc tggccgctgg gggcctggcc acgattgtgc tggggctggg cagcagcctg
    22021 ctctttgtgc gcagctttct ggccgggcgg caggggccgg tgcgcgctcc tctggtgcgg
    22081 ggcggtgcgt cggcgacttc ctcgtcgagc ggagcctcta cggccacggc gaacgcggga
    22141 agtggtgcag gcacgagcgg cggcacgatc attgcccgta aggcgtcttt ggccagcaac
    22201 agtgctcttt cctttacggt tcccagcaca ggcgatcctg gcattcttgt ccatctgccc
    22261 tcagatcagt ttgttgccta cgacgctgtc tgcacgcatg cgggctgcca ggttgaatat
    22321 gatccttcca gtcagttgct gatttgcccc tgtcacggtg ctacttatga tcccgcccag
    22381 ggggcgcgcg tggtgagtgg gccggccccc gcgccactga gtagcgtctc gatccgcatt
    22441 gatagcgctg gtgatatcat cgccctcccc tgagcggact gggcgtcgcg ctccggcgct
    22501 gggccgctca ctccggccag gccggctcgc gcgaaccgcc ttgtctctct cccccattgg
    22561 cggctcggcg gcctcgccgc cccgggcgtc aatggggctc gtgctattgt gagtaactca
    22621 ccatatgagg taaaatagag gaacagatag agggcggaaa caccattgcc tgctgctttc
    22681 ccaacgtttt tgttttatcc tgctttttcc tgcatagcga gcagaggcta tgcgttcagg
    22741 caggcccgcc gcgcagacaa gcccgcgttg gggcaggctt gttgagcgag cagagcggcc
    22801 cgcagccaga ctcagacagg aactggagag agaagtatgc caaagtccat gtttgagaag
    22861 atctgggagg cgcatgttgt gcgcgacgtt cccggagagt caactattct ctatatcgac
    22921 cgtcacctgg tgcatgaggc gacctcgccg caagccttcg ctggcctcaa ggcggcggga
    22981 cgcaaggtca ggcgccccga tgccacgatt gctgtgcttg accacaatgt accgaccctc
    23041 caggtgcgcg gcaggcgggt gctggagctt gtggaccagg cgagcgcgct ctgcatcacg
    23101 acgatggagc agcacgcccg cgagttcggc attacgctct tcgatatgaa cgacgagcgt
    23161 caggggatcg tgcacattat cggccccgag cagggcctga cgcttcccgg gatgaccatc
    23221 gtctgtggcg attcgcacac ctcgacgcat ggggcagtag gcgcgctggc ctttggcatc
    23281 ggcaccagcg agatcgagca tgtgctggcg acgcagtgcc tgttgcagaa gaagccgcgc
    23341 tcgatgaata tccgcatcga ggggagcctt ggcccgggca ttaccgccaa ggatatcgcg
    23401 ctctatatga ttgggcgcct gggcacgggc gtgggcacgg gccatgtggt cgagttcagc
    23461 ggctcggcgg tgcgcagtct ctcgatggag ggccgcatga cgctctgtaa catggccatt
    23521 gaggcgggtg cgcgggctgg catggtggcc cccgatgaga caacctttgc gtatctgaaa
    23581 ggccgcccct tcgctcccaa aggcgccctc ttcgagaagg cggtcgaggc ctggaagcag
    23641 ttgccgagcg atcccggtgc ccacttcgat acggtgcacg agatctcgat cgatgggctg
    23701 gccccgcaag tgacctgggg caccaatccg gggatggtgg tcgatgtgac cggtcgggtg
    23761 cccgatcccg ccgatgaatc tgatccgcag aagcgccgtg cctatgagcg cgccctggag
    23821 tatatgggtc tggagccagg ccagaagatt actgatatcc gcgtcgatac ggtctttatt
    23881 ggctcctgca cgaactcgcg cctggaagac ctgcgcctgg cggcgcagat ggtcaagggc
    23941 cgccatgtgg cgccgggcgt gcgggccctg gtggtgcccg gttcgatgca ggtgcggcgc
    24001 atggctgagg aggagggcct ggccgatatt ttccgcgagg ccggtttcga gtggcgcgcc
    24061 tccggctgta gcatgtgcat cgccatgaat ggcgaccgct tgagcgaggg cgagcgctgt
    24121 gcctccacca gcaaccgcaa tttcgaaggc aggcagggca agggtggacg cacccatctg
    24181 gtgagtcctc caatggccgc cgccgccgca atcgcgggcc actttgtcga tatccgcgat
    24241 ttctaggatc aacggctctg gtctggacct gaccagcagc gcggacagag atagcgacga
    24301 gcgcaaagag agagcgcctt gcagaaggag gcagagcaag acaatggaac cctttacccg
    24361 cctgaagggg ctggtcgccc cgctgaatcg aacgaacgtc aatacggatg agattacgcc
    24421 ggcccgcttc ttgaagacga ttagccgcac cggcttcgcc caggccctgt ttgctaactg
    24481 gcgctacctg ggcgacgatc agacgcccaa ccccgatttc gtcctcaatc agccccgcta
    24541 tcagggggcg acgatcctgc tggccggcga taatttcggc tgcggctctt cgcgcgaaca
    24601 tgccccctgg gcgatccgcg agtatggctt ccgctgcgtg atcgcgccca gcttcgccga
    24661 tatcttctac aataactgct tcaacaatgg cattctgccg gtgacgctcc ccgagccaac
    24721 ggtgctggag ctgttcaagg aggtcgaggc gaccgagggc tataccctgg aggtcgatct
    24781 ggaggcccag acggtgacga cgcccgacgg gcgtgtcttc cagttcccga tcgatgcttt
    24841 ccgcaagcag gccatgctgc agggcctcga caatatcggc tggacgctct cgcatctcga
    24901 tgagatccgt gcttttgagg agcgtcggca gcaagaagct ccctgggtct tcagcttgaa
    24961 gtgacaaggg tgtcactctt ccgttgtggt ctcggctgcg ccgccctctg tcccgccttc
    25021 cgccgccggg ctgtctgtgg ccgctcgatt ggccaggcaa ggccagtcag gaggatatga
    25081 cggagcagag cggcgcggct gcgagcaagg acgtcagcag accggtcact ccgccgcgga
    25141 tgtgggtgcg gccagcgagc gtcaggctgc ggcccgcctc cgcgcggggc gaatatccaa
    25201 gaaaaggagc gcaaagtggc gattcaccag attgatcccg gtctggtccc ttttcagatt
    25261 cttgatcccg agggcaacct ggtgggagag atgcctgctc tctcgccgga gcgcctactc
    25321 tatttctatc ggatgatgcg cctggcgcgg gccttctcgg accgcatcat tgctctgcag
    25381 cgtcaggggc gggccacgac cttcggctcc ctgactggtc aggaagcggc caccgtgggg
    25441 ctggccgcgc ctttgcagcc ccaggactgg ctggcgacct cctaccgtga gattgcctcg
    25501 ttgatcgtca agggtgttcc gctgagtacc ctcgtctatt ccttccgggg ctatactcct
    25561 cccaat
//
