LOCUS       NZ_MCIF01000002        21662 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Thermogemmatispora tikiterensis strain T81 A4R35_scaffold00002,
            whole genome shotgun sequence.
ACCESSION   NZ_MCIF01000002
VERSION     NZ_MCIF01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Thermogemmatispora tikiterensis
  ORGANISM  Thermogemmatispora tikiterensis
            Bacteria; Bacillati; Chloroflexota; Ktedonobacteria;
            Thermogemmatisporales; Thermogemmatisporaceae; Thermogemmatispora.
REFERENCE   1  (bases 1 to 5961682)
  AUTHORS   Tomazini,A., Lal,S., Stott,M., Henrissat,B., Polikarpov,I.,
            Sparling,R. and Levin,D.B.
  TITLE     Analysis of Carbohydrate Active Enzymes in Thermogemmatispora T81
            Reveals Carbohydrate Degradation Ability
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5961682)
  AUTHORS   Tomazini,A., Lal,S., Stott,M., Henrissat,B., Polikarpov,I.,
            Sparling,R. and Levin,D.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2016) Faculty of Agricultural and Food Sciences,
            University of Manitoba, 66 Chancellor's Circle, Winnipeg, Manitoba
            R3T 5V6, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-MAY-2011
            Assembly Method                   :: Newbler v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 25.9x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003268475.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 03:19:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,660
            CDSs (total)                      :: 4,607
            Genes (coding)                    :: 4,541
            CDSs (with protein)               :: 4,541
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 8 of 66
            Pseudo Genes (incomplete)         :: 56 of 66
            Pseudo Genes (internal stop)      :: 6 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            CRISPR Arrays                     :: 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:11:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1722756
            Orig. end                         :: 1744418
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MCIF01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21662
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ranthipeptide"
                     /region_number="6"
                     /rules="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085
                     or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /tool="antismash"
     cand_cluster    1..21662
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /kind="single"
                     /product="ranthipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21662
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11662](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11662)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
     gene            complement(151..558)
                     /locus_tag="A4R35_RS06935"
                     /old_locus_tag="A4R35_06885"
     CDS             complement(151..558)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714707.1"
                     /locus_tag="A4R35_RS06935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06885"
                     /product="RidA family protein"
                     /protein_id="WP_112427815.1"
                     /transl_table=11
                     /translation="MELQERQIVSSGSPYEPVIGFARAVRLGNMIAVSGTAPLGPDGKT
                     VAPGDAAAQTRRCLEIMQAALNQLGADLSTVIRTRIFLVHAEDWEQVGRVHGEFFGAVR
                     PASTMVQVARLLDPQWLVEIEADALLPTGAP"
     misc_feature    596..610
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.69"
                     /tool="antismash"
     gene            complement(649..1707)
                     /locus_tag="A4R35_RS06940"
                     /old_locus_tag="A4R35_06890"
     CDS             complement(649..1707)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A4R35_RS06940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A4R35_06890"
                     /product="hypothetical protein"
                     /protein_id="WP_112427817.1"
                     /transl_table=11
                     /translation="MSQQQMMFPPAEGPKPEGEEQGQAQAEGEPRYPAPSFFEASYAAH
                     EAFGKVQPGSSSLPAWQLALIIVVLVIIGSLFVSYLNSLLGLVLALAALIVLGGVVVLW
                     GFRRVITLPPRCFEVTGRPTLIVRNPAGPISIHRGGSHTNTVEILATKYLSGLWGGRDE
                     LTVDCQQRENTVSVAVSNLYQHPFSLTNLGYVRLDISVPAVCDLQIEGNAGSVQILGGQ
                     GRLTVKTNAGTITVLQATLEDRSSLETDAGTITVQQSRLCGQVYCHTNAGAISFGGALE
                     PGGHYQMTTNAGTIDVSLPPDTSLLLAASSNLGSVTNEFGQTLVGEPPRANLQLHTNLG
                     TVTVRRTGLSMH"
     gene            2330..3322
                     /locus_tag="A4R35_RS06945"
                     /old_locus_tag="A4R35_06895"
     CDS             2330..3322
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A4R35_RS06945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A4R35_06895"
                     /product="hypothetical protein"
                     /protein_id="WP_112427820.1"
                     /transl_table=11
                     /translation="MIQTLPLVLSIVMSELAIGSFLLLYVLDWRSEVRRSFLLFFTIIY
                     LLFTALTYLFQQSFASQPLLDSFERLDKQWTGYTSPVLLAFLLLLLPYLLFLILDRKAG
                     VDGKEKAAQAGEGQFSVIRVLRLVSGGLASAAGLLMLFVLGMIYRPLAPSPLGEALTVL
                     SFFIAALAVGGVMTSMWLGHWYLVTPALSERPLLFSTAVVLIAIVLQILLTLASSTVAS
                     PATSTAATHSAQVTATAIATSPTPAPAVSSASAAVKPAGAPTVSPLSMDVLGWLRILLG
                     FAFPLVLGALTWKLARERSFQSATGMLYLIVVMTLAGEVLARGMFLSTL"
     gene            complement(3634..5253)
                     /locus_tag="A4R35_RS06950"
                     /old_locus_tag="A4R35_06900"
     CDS             complement(3634..5253)
                     /NRPS_PKS="Domain: AMP-binding (26-434). E-value: 4.3e-111.
                     Score: 363.3. Matches aSDomain:
                     nrpspksdomains_A4R35_RS06950_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.5"
                     /locus_tag="A4R35_RS06950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06900"
                     /product="AMP-binding protein"
                     /protein_id="WP_112427823.1"
                     /sec_met_domain="AMP-binding (E-value: 9.3e-118, bitscore:
                     391.4, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEKMFAPELFRPPVEFPEVPYDHLLRRAAERNPDGRAIIYHDLS
                     LTYREVVSMVNCIANGLYNLGLRKGERLCIFTLNRPEYTITFIAAASIGLVVTPMNPSY
                     KEREIAYQLENSEASAILVQRELLPLLQAVLAQKSLPNLRHVIVTGSHVPEGLPQAIPF
                     GRLLRESSPKAPPTVPIDPDDLLALPYSSGTTGLPKGTMLTHRNLVSNNLQFTTALQTT
                     FTDVALLFLPFYHIYGVMLTGGFLACGATQIVMERFDMLQSLELCEKYNVTYYFAVPPI
                     ILALANAPIDLSKLKSVKYIFSGAAPLAPEPARKVQEKTGIQVVQGYGLTEASPLTHAQ
                     PKDPALVRLESVGLPVHNTEQKIVDLETGTRELPVGEDGEIIIRGPQVMKGYWKAPEET
                     AQTLRDGWLYTGDIGHVDEDGYTYIVDRKKEMIKYKGFGIAPAEIEALLLEHPAVMDAA
                     VIPVPDPEAGEVPKGFVVLRPGQSVTPDDLIAFVNGRLAGYKRLGFVEFIDSIPKVASG
                     KILRRELKERERARRAAEQQAL"
     CDS_motif       complement(3919..3981)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS06950_0003"
                     /evalue="2.00E-03"
                     /label="NRPS-A_a8"
                     /locus_tag="A4R35_RS06950"
                     /protein_end="445"
                     /protein_start="424"
                     /score="11.0"
                     /tool="antismash"
                     /translation="VDRKKEMIKYKGFGIAPAEIE"
     aSDomain        complement(3952..5175)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R35_RS06950_AMP-binding.1"
                     /evalue="4.30E-111"
                     /label="A4R35_RS06950_AMP-binding.1"
                     /locus_tag="A4R35_RS06950"
                     /protein_end="434"
                     /protein_start="26"
                     /score="363.3"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="RRAAERNPDGRAIIYHDLSLTYREVVSMVNCIANGLYNLGLRKGE
                     RLCIFTLNRPEYTITFIAAASIGLVVTPMNPSYKEREIAYQLENSEASAILVQRELLPL
                     LQAVLAQKSLPNLRHVIVTGSHVPEGLPQAIPFGRLLRESSPKAPPTVPIDPDDLLALP
                     YSSGTTGLPKGTMLTHRNLVSNNLQFTTALQTTFTDVALLFLPFYHIYGVMLTGGFLAC
                     GATQIVMERFDMLQSLELCEKYNVTYYFAVPPIILALANAPIDLSKLKSVKYIFSGAAP
                     LAPEPARKVQEKTGIQVVQGYGLTEASPLTHAQPKDPALVRLESVGLPVHNTEQKIVDL
                     ETGTRELPVGEDGEIIIRGPQVMKGYWKAPEETAQTLRDGWLYTGDIGHVDEDGYTYIV
                     DRKKEMIKY"
     CDS_motif       complement(4051..4134)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS06950_0002"
                     /evalue="5.00E-09"
                     /label="NRPS-A_a6"
                     /locus_tag="A4R35_RS06950"
                     /protein_end="401"
                     /protein_start="373"
                     /score="28.5"
                     /tool="antismash"
                     /translation="PVGEDGEIIIRGPQVMKGYWKAPEETAQ"
     CDS_motif       complement(4639..4689)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS06950_0001"
                     /evalue="1.40E-05"
                     /label="NRPS-A_a3"
                     /locus_tag="A4R35_RS06950"
                     /protein_end="205"
                     /protein_start="188"
                     /score="17.8"
                     /tool="antismash"
                     /translation="PYSSGTTGLPKGTMLTH"
     misc_feature    complement(5234..5245)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.15"
                     /tool="antismash"
     gene            5627..7258
                     /locus_tag="A4R35_RS06955"
                     /old_locus_tag="A4R35_06905"
     CDS             5627..7258
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025681.5"
                     /locus_tag="A4R35_RS06955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06905"
                     /product="GxGYxYP domain-containing protein"
                     /protein_id="WP_112427825.1"
                     /transl_table=11
                     /translation="MDAERGAASFSLDWPVNAFFPQLQVPHNLAVADLRNASFAVKLAA
                     STLAGLVNVPQPQLYLATYDDDLFWLQSAIAPWPVQLERLEAQGETLLRELIARFQARI
                     AGFIVYDPQRPASINVATTLAGIQAGVVAAPELLPLLQAAGRKPVLRDLRLQGWESESL
                     AYRWARETSQTQRSRRSLAGMNPEIASGLRSFLVATRTFVHWLDSRPWQPSAGQQRLLL
                     EELLAELLPGSIHLGWFPDEGSGVTLASERAVAVLASDHCSNLEVWTALQPPELIERLQ
                     ALAQRYHRQQDERPLPALEPRVYLSLTISDGDNLQYVQHRMLHLWRDSARGRFPLGWTI
                     APALLEAAPALAAYYLETASPQDELIAGPSGAAYMFPSSWPAAQRPAFLEHSQRLLSGM
                     GLSLLQVLDGDWRQRLGLPLPASMRLSNVEEQHAFIEALRPAGLRGLLTGASGLTLSYK
                     KIMGMPVYHNLGLVSSRSQLVHLVRGLQATHRRRPLFLQLYVLAWRLTPSALWEALQEL
                     REIAYPVTPGQLLQLIAQQGAGGAES"
     gene            7258..8922
                     /gene="mftF"
                     /locus_tag="A4R35_RS06960"
                     /old_locus_tag="A4R35_06910"
     CDS             7258..8922
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR03965.1"
                     /locus_tag="A4R35_RS06960"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969).; Members of this protein family, MftF,
                     are glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06910"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_112427827.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.9e-31,
                     bitscore: 107.3, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPQSYYYPSPGCYRLAPGLCLLEEIDGRPGGLVIASYPLRVLRL
                     GQQAWRLLNLCRQERSSEELVRLSGLAPARVATLCQQLSAKGLLEAGPPLPPRSWPGVS
                     VIVPTCNRVRQLERCLQAILELRYPSQALEIIVADDASRDQTPTLLTRFAPAFASRGVR
                     LHCLRHARRQGAAGARNSGAAAARYDLLAFVDDDCVVTPDWLTTLVPLFSETSVAAVGG
                     QIRAYETGSALGRYEDVRSSLFMGLQPQEVRLSGPLTYLPTACLLVRREAWQALDGFAP
                     LSCGEDVDFCYRLLAQGWRIRYWPAPESVVYHDYRTRLGTFLGTRVRYASAEAVLQQLH
                     PQQRRILLLPPWEASFAALTLGSLWQLITALPLGSLLWTRSQGRQRPSRTGQARLSLLL
                     AFPALLLPGASTWRRHQAARLARLPLTAWQIWQATLRSYLAYTYHLCRHLTRYYTLALL
                     AAGLLFPPLLVLVGLLQAVVVGVDYARLRPRLSLPAFALYSLLEDCAYALGLLLGCWRQ
                     RTWRPLLAVIEWKNATVTQLAQPSEPGPPSLPSRDQR"
     gene            8951..9772
                     /locus_tag="A4R35_RS06965"
                     /old_locus_tag="A4R35_06915"
     CDS             8951..9772
                     /NRPS_PKS="Domain: Abhydrolase_1 (28-256). E-value:
                     1.8e-19. Score: 62.2. Matches aSDomain:
                     nrpspksdomains_A4R35_RS06965_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024109.5"
                     /locus_tag="A4R35_RS06965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06915"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_112427828.1"
                     /sec_met_domain="PF00561 (E-value: 7.8e-16, bitscore: 56.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.4e-19, bitscore:
                     69.6, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGRLEPEWGFIEARGTRLYYEVAGRGHPLVLLHSGLLDHRSWDP
                     QFMEFARYYEVVRYDRRGFGRSAMGNVEYSDVRDLYDVLNDLAIDRAYLLGLSRGGRLA
                     IDFALSYPEMVDALILAAANISGYDNYMPETLERGKRIAAAARAQDKEQLYELWATDPA
                     MPHEDEYPEAAQRYRELLRDYSFVHYLNPVPSEQLRPPALQRLAEIRQPTLILIGDADS
                     LEMQAQAELLEAGIIDARKVVIRGARHMLNLERPETFNRAVLTFLHSLERR"
     aSDomain        9035..9718
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R35_RS06965_Abhydrolase_1.1"
                     /evalue="1.80E-19"
                     /label="A4R35_RS06965_Abhydrolase_1.1"
                     /locus_tag="A4R35_RS06965"
                     /protein_end="256"
                     /protein_start="28"
                     /score="62.2"
                     /tool="antismash"
                     /translation="PLVLLHSGLLDHRSWDPQFMEFARYYEVVRYDRRGFGRSAMGNVE
                     YSDVRDLYDVLNDLAIDRAYLLGLSRGGRLAIDFALSYPEMVDALILAAANISGYDNYM
                     PETLERGKRIAAAARAQDKEQLYELWATDPAMPHEDEYPEAAQRYRELLRDYSFVHYLN
                     PVPSEQLRPPALQRLAEIRQPTLILIGDADSLEMQAQAELLEAGIIDARKVVIRGARHM
                     LNLERP"
     gene            complement(10001..11227)
                     /gene="mftC"
                     /locus_tag="A4R35_RS06970"
                     /old_locus_tag="A4R35_06920"
     CDS             complement(10001..11227)
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: SPASM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03962.1"
                     /locus_tag="A4R35_RS06970"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="A4R35_06920"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_112427830.1"
                     /sec_met_domain="PTHR43273 (E-value: 1.2e-43, bitscore:
                     147.6, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.7e-27, bitscore: 94.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 6.9e-08, bitscore: 30.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 6.1e-08, bitscore: 30.5,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.1e-13, bitscore:
                     49.5, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 3.9e-08, bitscore: 31.8,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQFVQFHGRRHQWTGSQRRAEPGALHPLARAAFAETGDPLLPAQ
                     TSGSQRFLRGGLSAPITVTWEITAACNLTCVHCLSSSGKRRPRELTTEQALAVVDELAE
                     MQVFQIHFGGGEPFMYPGIWRVLERCRERDLVMCVSTNGTLITPERAQRLAALEPIYFQ
                     VSLDGATPETNDRIRGQGAFRRAMRGLERLAAEGLSLTVNSVLTRYSFPELDRLYAIAG
                     NFGAKLRVTRLRPSGRGRAVWEELHPTREQYRSFVAWLREHPDVLTADSFFHLNAFGER
                     LAGLDMCGAATATCCICPEGEVYPCAFFQAPEFEAGNLHEQSFREIWQHSPVFAFYRQM
                     GTGSCGGCVRYDLCHGGCPATKWFVQRSLQVRDPECVLAGSSVDPLPASHEAPCSSAAM
                     WGSALPRQA"
     gene            complement(11333..11662)
                     /gene="mftB"
                     /locus_tag="A4R35_RS06975"
                     /old_locus_tag="A4R35_06925"
     CDS             complement(11333..11662)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03967.2"
                     /locus_tag="A4R35_RS06975"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="A4R35_06925"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_112427832.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 4.2e-11,
                     bitscore: 41.0, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTMTAHAAGYRYRLLPEVKIRREAFGGILYKYGCADRGMLSFVNS
                     PRLIDLLLLAREQFAGDLSAAIEQQVSSAGARARLLAALDELRKRGYLEAEVIAGLAPE
                     ETAAE"
     aSDomain        complement(11384..11632)
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_A4R35_RS06975_Mycofactocin_RRE.1"
                     /evalue="2.60E-13"
                     /identifier="RREFam016.1"
                     /label="A4R35_RS06975"
                     /locus_tag="A4R35_RS06975"
                     /protein_end="93"
                     /protein_start="10"
                     /score="41.0"
                     /tool="antismash"
                     /translation="RYRLLPEVKIRREAFGGILYKYGCADRGMLSFVNSPRLIDLLLLA
                     REQFAGDLSAAIEQQVSSAGARARLLAALDELRKRGYL"
     gene            12049..13683
                     /locus_tag="A4R35_RS06980"
                     /old_locus_tag="A4R35_06930"
     CDS             12049..13683
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916793.1"
                     /locus_tag="A4R35_RS06980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06930"
                     /product="penicillin-binding protein 2"
                     /protein_id="WP_112427834.1"
                     /transl_table=11
                     /translation="MNINSAIRRLNQIFIVLFLGLSLGLVYWQVAVAQQVTANIHNIRQ
                     CMKDHAPVRGKIYDRNGVLLAESKPIDPNNPFKGVLCGYQRHYYLDGMPSLANLIGFYI
                     SPLFNSTGIEHAFDDYLSGRAGLTELTNNLNLILHRPPVGDNIYLTIDTRIQKIVERDF
                     DKYAPSYNPPVPELKIDNDNVFATNRGAVIVTDPHTGEILAMVTRPTYDSNRVAAGDLA
                     YYNQLLRNPDAPLLERPVQGQYVPGSVYKAMTLLAALDTGTYQLNTPSFDYQHAVGPVT
                     IGGEVFAPQMSNIYHASYARTFPVSMNMGFSHSDNVMFAFIGSKLGADTWLEYNKRFYV
                     GQDIPFDLPVARSSVLPPNGQLPPNLLAENSFGQGVDLVTPMLISLIDDAIANNGELMR
                     PMLVSRIQAPDGTIIKSMSPQTLSTPISEQTAASAREAMYGVVRCGSGSVIPELYNSPW
                     NIIAKTGTGQVGGGKAAHGWLMTQAPYQDPRLTIVSMRENAGEGANVNGPAVAAMYNDI
                     YTSVLKLQQPAPADSSVPYCTQHGLWT"
     gene            13940..14131
                     /gene="mftA"
                     /locus_tag="A4R35_RS24195"
                     /old_locus_tag="A4R35_06935"
     CDS             13940..14131
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: protein motif:HMM:TIGR03969.1"
                     /locus_tag="A4R35_RS24195"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06935"
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_189361445.1"
                     /transl_table=11
                     /translation="MESLFEHNRYFPEAEEQSAYSQTEPVLPSSLVDEQTTGAEDEEEF
                     EEELIIEDFTIDGICGVY"
     gene            14109..14975
                     /locus_tag="A4R35_RS06990"
                     /old_locus_tag="A4R35_06940"
     CDS             14109..14975
                     /NRPS_PKS="Domain: PKS_KR (18-191). E-value: 1.2e-13.
                     Score: 43.3. Matches aSDomain:
                     nrpspksdomains_A4R35_RS06990_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566068.1"
                     /locus_tag="A4R35_RS06990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06940"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_112427838.1"
                     /sec_met_domain="adh_short (E-value: 8.8e-35, bitscore:
                     118.4, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASAESTSPAAEGRFADRVVLITGAARGQGRAHALAFAREGARLV
                     ICDACRQYRTVPYPLASPEELTAVAREIEALERPVIAAQVDVTDLPAMEALAERTRREL
                     GPIDVVVANAGLYSFALSWQMTEEQWDETINVDLKGVWITCKVAIPQMLERRQGKIICI
                     GSTASFRGMDHLAHYVAAKHGVIGLVKTLARELAPYNINVNAVCPTSVATTMCLNQALY
                     DLFAGGPGPQATYEHMVSQMNRMNLFSDRNLLPPEDVSAVVLWLASDEARHLTGCALPV
                     DAGYLTR"
     CDS_motif       14163..14243
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS06990_0001"
                     /evalue="3.00E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="A4R35_RS06990"
                     /protein_end="45"
                     /protein_start="18"
                     /score="10.3"
                     /tool="antismash"
                     /translation="VVLITGAARGQGRAHALAFAREGARLV"
     aSDomain        14163..14681
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R35_RS06990_PKS_KR.1"
                     /evalue="1.20E-13"
                     /label="A4R35_RS06990_PKS_KR.1"
                     /locus_tag="A4R35_RS06990"
                     /protein_end="191"
                     /protein_start="18"
                     /score="43.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VVLITGAARGQGRAHALAFAREGARLVICDACRQYRTVPYPLASP
                     EELTAVAREIEALERPVIAAQVDVTDLPAMEALAERTRRELGPIDVVVANAGLYSFALS
                     WQMTEEQWDETINVDLKGVWITCKVAIPQMLERRQGKIICIGSTASFRGMDHLAHYVAA
                     KHGVIGLVKT"
     misc_feature    15195..15209
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 20.35"
                     /tool="antismash"
     gene            15263..15721
                     /locus_tag="A4R35_RS06995"
                     /old_locus_tag="A4R35_06945"
     CDS             15263..15721
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627194.1"
                     /locus_tag="A4R35_RS06995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06945"
                     /product="DIP1984 family protein"
                     /protein_id="WP_112427840.1"
                     /transl_table=11
                     /translation="MKLAEALVLRSDMKKQLAQLRNRLKRSAIVQEGDQPPENPEILLR
                     EVEELLRQYRQLMVRINRTNLESRLPDGRTLTEALAERDVLDLHHSIISDLAAAASERF
                     DRYGRAEIRRLPTVEAAALYRQLDRIARQRRELDTLIQATNWATDLQE"
     gene            complement(16082..17050)
                     /locus_tag="A4R35_RS07000"
                     /old_locus_tag="A4R35_06950"
     CDS             complement(16082..17050)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425110.1"
                     /locus_tag="A4R35_RS07000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06950"
                     /product="VOC family protein"
                     /protein_id="WP_223258239.1"
                     /transl_table=11
                     /translation="MKEQQENTPINAADMLPATTHIGLVALTVADLERERRFYEEVLGF
                     QLIDRSERQIWLGAADLAGQTEEPIMKKQPLLLLIEQAEAPRPPSWSTGLYHFAILVPS
                     RPALARSLAQLAASRYPLGGYADHLVSEALYLSDPEGNGIEIYRDRPRETWRWQSGQVA
                     MATDPIDVRAMLTEGRAELERQPWRGLPAGTTIGHVHLRVADLRQAEHFYHQLLGFAIT
                     ARMPGALFVSAGGYHHHLGLNTWESRGASAPPEGSLGLRLFSIELPTRAEIERVAERLE
                     RERWPIERHGEIIAVRDPWHHLVLLTPEGKRQPDDLATWLA"
     gene            17480..18586
                     /locus_tag="A4R35_RS07005"
                     /old_locus_tag="A4R35_06955"
     CDS             17480..18586
                     /NRPS_PKS="Domain: PKS_ER (18-360). E-value: 8.4e-14.
                     Score: 43.3. Matches aSDomain:
                     nrpspksdomains_A4R35_RS07005_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873304.1"
                     /locus_tag="A4R35_RS07005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06955"
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="WP_112427851.1"
                     /sec_met_domain="ADH_N (E-value: 6.9e-31, bitscore: 104.5,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2.2e-29, bitscore:
                     100.2, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTRTAVIYEPGTPIRVEEIELDTPKEQEVLVRMVAAGVCHSDYH
                     VVKGDLPAYLPMALGHEGAGIVEQVGPGVSDLKPGDHVLMSFIPACGSCPSCVSGHSNL
                     CDKGAGTLSGPLLDGTFRMHGEKGDIGQFCYIGSFSEYTVVPAMSVVKIADYYPLNRAV
                     LVSCGVPTGVGSVIHRARVTPGSTVMVIGCGGIGMNIVQGAAIAGARMIIAVDIHDFKL
                     EKAKEFGATHTINSSQEDPVNKALELTWGVGVDYAFEAIATPETIAQAFNATAKNGTVV
                     VAGLSPFLAQAIPISPLNLVLYQKTLMGTLFGDSQPRNDIPMLLRMYESGKIKLDELVT
                     RTYKLDQINEAYDDLLAGRNIRGVIEFS"
     aSDomain        17534..18559
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R35_RS07005_PKS_ER.1"
                     /evalue="8.40E-14"
                     /label="A4R35_RS07005_PKS_ER.1"
                     /locus_tag="A4R35_RS07005"
                     /protein_end="360"
                     /protein_start="18"
                     /score="43.3"
                     /tool="antismash"
                     /translation="EIELDTPKEQEVLVRMVAAGVCHSDYHVVKGDLPAYLPMALGHEG
                     AGIVEQVGPGVSDLKPGDHVLMSFIPACGSCPSCVSGHSNLCDKGAGTLSGPLLDGTFR
                     MHGEKGDIGQFCYIGSFSEYTVVPAMSVVKIADYYPLNRAVLVSCGVPTGVGSVIHRAR
                     VTPGSTVMVIGCGGIGMNIVQGAAIAGARMIIAVDIHDFKLEKAKEFGATHTINSSQED
                     PVNKALELTWGVGVDYAFEAIATPETIAQAFNATAKNGTVVVAGLSPFLAQAIPISPLN
                     LVLYQKTLMGTLFGDSQPRNDIPMLLRMYESGKIKLDELVTRTYKLDQINEAYDDLLAG
                     RN"
     CDS_motif       17654..17692
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS07005_0001"
                     /evalue="5.40E-03"
                     /label="PKSI-ER_m2"
                     /locus_tag="A4R35_RS07005"
                     /protein_end="71"
                     /protein_start="58"
                     /score="9.8"
                     /tool="antismash"
                     /translation="LGHEGAGIVEQVG"
     misc_feature    complement(18726..18739)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.44"
                     /tool="antismash"
     gene            19017..20180
                     /locus_tag="A4R35_RS07010"
                     /old_locus_tag="A4R35_06960"
     CDS             19017..20180
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1124: acetyl-CoA acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098292.1"
                     /locus_tag="A4R35_RS07010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06960"
                     /product="thiolase family protein"
                     /protein_id="WP_112427853.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.3e-05, bitscore:
                     23.0, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKHQEAVIVAAVRTPIGIGKPDRGKLTGIRPDELAAMTLAEVVRR
                     AGIEKGLVEDVIMGCVTQIGEQGLNIGRVAPLIADFPVETCGVSVNRMCGSSEQAIHFA
                     AQAIISGTIDVAIAAGVESMSRVPMGSDKAGASFSEKLIARYPLVPQGISAELVAEKWH
                     ITREEADAFSLESHRRAAAATAEGRFKPEIMPIEVTLPDGSREVMISDQGIRPNTSLEQ
                     LAALKTVFKPDGIITAGNSSQISDGAAAVLLMSDSKAQELGLKPLARIVAMATAGVDPV
                     LMLSGPIPATQKVLKKAGLRLADIDLVEINEAFASVPLAWARELDPDMSRVNVNGGAIA
                     LGHPLGASGARLMTTLVHELQRRGGRYGLQTMCIGHGMATATIIERI"
     gene            20184..21290
                     /locus_tag="A4R35_RS07015"
                     /old_locus_tag="A4R35_06965"
     CDS             20184..21290
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012656.5"
                     /locus_tag="A4R35_RS07015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06965"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_112427855.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 3.8e-22, bitscore:
                     77.2, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 3.1e-13, bitscore:
                     47.8, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 6.3e-23, bitscore:
                     79.7, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDFAPTEEQEEIRKLAQSIALDHLRPHARKAEIEHDSPNELWLT
                     LAQTGLTTPFPEEYGGSGPLEAVTYSLIAEELAYGDPGLALHIVGSLMGPLTVWLAGSR
                     EQQERYLPPFGNSTSGYLQRGSLAFAERTGGYTLADIHLQARRTSDGYLLNGTKRDVVH
                     GQRADLRVVLARLAEHESEGPEGKLCAFVLPGTQEGLSIQAEEEKLGLQAAPSATYLFN
                     QVQLPAEALLGETGSSGVVRAATLYQILRAAIACGTARAAFEYCSAYARERIAFGRPIV
                     SYQGIAFIIAEMAMKLDAARLLTWRAACSWDRGEADETLIHEAESAQRQAVKMAQAATT
                     DAIQVMGGAGFMQDHPAEMWMRDAAAME"
ORIGIN
        1 agcgcgaggg caggtagatc gcctatctcg cccgcttctc gcgctctagt ctagaggagt
       61 tattgtttcg ctgtcaattg cttgctcagc ttgtacgctc ggggtcatgc taccattcgg
      121 gcccgggcag cgccttgcga cggtctcctt ctacggagcg cctgtcggaa gcaaagcatc
      181 ggcctcaatc tctaccagcc actgtggatc aagcaagcgt gccacctgga ccatcgtaga
      241 ggccggacgc accgcgccaa agaactcacc atgaacgcgg cccacctgtt cccagtcttc
      301 ggcatgcacc agaaagatac gggtgcgaat cactgtcgag agatcggccc caagctgatt
      361 cagcgctgcc tgcataatct ccagacaacg tcgagtctga gctgcggcat cgccaggggc
      421 cacggtcttg ccgtccgggc caagcggcgc agtacccgag acggcgatca tatttccaag
      481 acggaccgcc cgcgcaaagc cgatcactgg ctcatagggt gaaccagagg aaacgatctg
      541 acgctcctga agttccatat actttcttac ttccccttta acctatgaaa gataattttt
      601 tctgttgatt aagaaaaaca ccagacggga taagggcctt gaggcttttc agtgcataga
      661 cagaccagtg cggcggactg tcacggtgcc aagattggta tggagctgga gattggcacg
      721 aggtggttca ccgacaagcg tttgcccaaa ctcattggta acgctgccca ggttggagga
      781 agcagccagc agcagcgatg tgtcgggtgg caagctgaca tctatcgtac ccgcgttggt
      841 cgtcatctga tagtgacctc cgggttccaa ggcaccgcca aaagagatgg ctccggcatt
      901 cgtatgacag taaacctgtc cacagagccg actctgctgc acagtgatgg tcccggcatc
      961 agtctccaga ctcgaccgat cttcaagtgt cgcctgtaac acagtaatcg tacccgcatt
     1021 cgttttgacc gtcaaccgtc cctgcccgcc caggatctgg acagagccag cattgccctc
     1081 gatctgcagg tcacagacag caggtacgct gatatccagc cgaacatagc ccaggttcgt
     1141 caacgaaaag ggatgctggt agaggttcga aaccgcgaca ctgaccgtgt tctcccgctg
     1201 ctgacaatcg acggtcagct catcgcgccc gccccagaga ccactcaggt acttcgtcgc
     1261 caggatctca accgtgttag tgtgacttcc tccacgatga atggaaatgg ggccagccgg
     1321 attgcgaacg atgagggtcg gacgccccgt gacctcgaag cagcgggggg gtagagtgat
     1381 tacccgtcta aagccccaga ggaccacaac accccccagt acaatgaggg cagccagggc
     1441 cagcaccagg ccaagtagac tgttgagata gctgacaaag agactgccga taattaccaa
     1501 aacgactatg atcagggcaa gctgccaggc cggaagagaa gaagagcctg gttgcacttt
     1561 tccaaaagcc tcgtgggctg catagctcgc ctcaaaaaag gaaggcgcag gatagcgcgg
     1621 ctccccctca gcttgggcct gcccttgctc ttcaccttcc ggctttggcc cctcagccgg
     1681 cggaaacatc atttgctgct ggctcatggc tggtttctcc ccctagcttc cgtcttcttc
     1741 tcctcttttt acttcctttc ttcgccaaca gggacagcct cagccccctg accgtcgctt
     1801 ccagcccgtt gaggggccag ggcacttgac caggccaggg agaaggcagt caccccccat
     1861 ccgtcctgac ccccggtcac caacggccag agcgcctact cttctcccct cccgactacc
     1921 cacccggtac ctgggaagca ggcggtcgcc ctcctgtctc ctagcggtag gtctcccgac
     1981 gccacagcac ccacaaagga gagcaacttc agatcgctga ccctctcttc cagggttcct
     2041 cgtctctgcc actgctgtag cgaatacgtg aggctgggag aaaagttgca aagccagtag
     2101 agcggtagac tgtcttccca gaacagtgac taggcagagc agaagaacag gcaatcaagc
     2161 aaagcagagg tcgatgagaa aagatctgcc ccggctcaca gccagattgt cccggctggc
     2221 ggaggaggag tataatctgt cagggtgagg gccgctgctg ccccctgttt ggctagactg
     2281 tcggcatcac cccaaaggaa gacgcgagct aaaaggatat gtttccggca tgatacagac
     2341 actcccactg gtgctttcga ttgtcatgag cgagctggcc atcggctcgt tcttgctgct
     2401 ctacgtgctg gactggcgta gtgaggtgcg gcggagcttt ctactctttt tcaccattat
     2461 ctatctcctc ttcaccgctc tcacctatct tttccagcag agcttcgcct cccagccatt
     2521 gctggatagc tttgagcgac tcgacaagca gtggactggc tataccagcc cggtgctgct
     2581 ggcctttctc ctgctactgc tgccctattt gctcttcctg atcctggacc ggaaagccgg
     2641 agtagacggt aaagagaaag ctgctcaagc cggggaaggg caattttctg tgatacgggt
     2701 tctgcgactg gtaagcggcg gcctggcttc agcggctggt ttgctcatgc tctttgtact
     2761 gggcatgatt taccgtccgc tcgcgccctc gccgctcggg gaagccctca ccgttctgag
     2821 cttcttcata gcggccctgg ctgtcggcgg cgtgatgacc tcgatgtggc tcggccactg
     2881 gtatcttgtc acgccagctc tgagcgagcg cccgctcctg ttcagcacgg cggtggtcct
     2941 gatcgccatt gtgcttcaga ttctcctgac gctggccagc tctactgtag ccagccctgc
     3001 gacctcaacc gccgccacac actcagctca ggtcacggcg acagccatag ctacgagtcc
     3061 gacgcctgca cccgccgtct caagcgcttc tgcagccgtc aagccagccg gcgctcccac
     3121 ggtcagtcct ttgagtatgg acgttcttgg ctggttgcgc attctgctcg gtttcgcctt
     3181 cccgctcgtc ctgggcgctc tcacctggaa gctggcgcgt gaacgctctt ttcaatcggc
     3241 cacgggtatg ctctatctga tcgtcgtcat gacgctggca ggtgaggttt tggcccgcgg
     3301 catgttcctg agcacgcttt agggcacggc tggcggggcc tgtcgcgcca cgaatcgtcc
     3361 agatcgagca caagcctgta aggaagtcac cagatcgcgg gtgcctcaca agacagggtg
     3421 agagtggcat ccaggcagat agatgccaga actctcaccc tgtttctgta ctcaaggtct
     3481 ctaacagacc cggcgttgac cctctttccc tgaaacgaac cctatccgca gcttgcttga
     3541 agcaggccgc tgatcagggc actggacagc caaacaagca agaaggccgg atggcttcag
     3601 cgcacggcct gttttttcgg cctggcgctc tggctagagg gcttgctgct cggcagcgcg
     3661 tcgcgcacgc tcgcgctcct tcagctcgcg acggagaatc ttgccagaag ctaccttggg
     3721 aatggaatca atgaattcca caaagcccag gcgcttgtaa ccagccaggc gcccattgac
     3781 aaaggcgatc agatcatcgg gcgtcacact ctgacctgga cgcaggacga caaagccctt
     3841 gggcacctcg ccggcctcgg gatcaggcac ggggatcacc gccgcatcca tgaccgcggg
     3901 atgctccagg agcagggcct cgatctcggc gggggcaatg ccgaagccct tgtacttgat
     3961 catctccttc ttgcgatcaa caatgtaggt gtagccgtct tcgtcgacat ggccgatgtc
     4021 acctgtatag agccagccgt cgcgcagcgt ctgagccgtc tcctcgggcg ccttccagta
     4081 gcctttcatg acctggggac cgcgaatgat gatctcccca tcctcaccca ctggcagctc
     4141 acgcgtgccg gtctcgaggt cgacaatctt ctgctcggta ttgtgcacgg gcaggccgac
     4201 gctctccagc cgcacgagag ccgggtcctt tggctgagca tgggtgagcg gcgaggcttc
     4261 ggtcaggcca taaccctgga ccacctgaat gcctgtcttt tcctgcacct tgcgcgccgg
     4321 ctccggtgcc agcggggccg ctccagagaa gatgtatttg acgctcttga gcttgctcag
     4381 atcaatggga gcattggcca gtgccaggat aattggagga actgcaaagt agtaggttac
     4441 attgtatttc tcacacagct ccagggactg gagcatatca aagcgttcca taacgatctg
     4501 cgtggctcca caggccagga aaccaccagt cagcatgaca ccatagatgt ggtagaaagg
     4561 caggaagagc agagccacat cggtgaaggt tgtctggagc gccgtggtaa attgcaggtt
     4621 attggagacc agattgcggt gtgtgagcat tgttcccttg ggcagaccag tcgtgcccga
     4681 ggaataggga agcgccagca ggtcatcagg atcgatcggg accgtcggtg gcgccttcgg
     4741 cgaggactcg cgcagcaggc gcccgaaagg gatcgcctgg ggcaggccct cgggtacatg
     4801 gctcccggta acgatgacat gcctcagatt gggcagcgac ttctgcgcca gcacagcctg
     4861 cagcagcggc agtaactcgc gttgtaccag gatggcactg gcctccgagt tctccagttg
     4921 ataggcaatc tcgcgctctt tataggaggg attcatcggg gtcacaacca ggccgatgga
     4981 ggccgctgcg atgaaggtga tggtatattc tggccgattg agggtaaata tgcagagccg
     5041 ctcgcccttg cgcagtccca gattgtacag tccgttggca atgcagttca ccatcgagac
     5101 aacctctcga taggtcaggc tgaggtcatg gtaaatgata gcccgcccat cggggttacg
     5161 ctccgccgct ctgcgcagca ggtgatcata ggggacttct gggaactcga cgggaggtct
     5221 gaacaactcg ggagcaaaca ttttctctgg catgcttcaa tcctttctcg acccggccct
     5281 gggtctcaac tgcaatgcag aatgcagatt ctcttgcaag cagtaacagc aggtgaaaaa
     5341 agctaccggg tattgaagcg acgcggtagc caggttctgc tcagcttcgc tgctttcgca
     5401 ggcttgtata gtagcatact ccagacatac gagctggtca atacagcatt cgttctggtt
     5461 tcatacattt cgaattatca gcaggtgagg ataagcttcc ttcccttgac gagggcagct
     5521 tgacacccta taatcctctc aaagaaagcg atgtcagcca gtgcccggtg aggccgcagg
     5581 cagcacctgg cgccggctcc cgtccagaaa ggacctgtcc ccccctatgg atgccgagcg
     5641 aggcgcggcc tcgttctctc ttgattggcc agtgaacgcc ttttttccac agctgcaagt
     5701 gcctcacaac ctggcagtgg ctgatctacg caatgcctcc ttcgctgtta agctggcagc
     5761 tagcacgctg gctggcctgg tgaacgtgcc acagccacag ctctacctgg caacctacga
     5821 cgatgatctt ttctggctgc aaagcgccat cgccccctgg cccgtgcagc tggaaagact
     5881 ggaagcgcaa ggggagaccc tgctcagaga gctgatcgcc cgctttcagg cgcgcattgc
     5941 tggcttcatt gtctatgatc cacagcgccc ggccagcatc aatgtggcga ctacgctggc
     6001 cggtatccag gcgggcgtag tggcagcccc cgagttgctg ccgctgctac aagcagctgg
     6061 gaggaagcca gtgttgaggg acctgcgcct tcagggctgg gagagcgaga gcctggccta
     6121 tcgctgggca agagagacca gccagaccca gagatcgcga cggtccctgg ccggtatgaa
     6181 tccagagatc gccagcggct tgcgctcctt cctggtagct acgcgcacct tcgtccactg
     6241 gctcgattcg cgcccctggc agccctccgc aggccagcag cgccttctcc tggaggaact
     6301 gctcgctgag cttctccctg gcagcatcca tctgggctgg ttcccggatg agggaagcgg
     6361 ggtaacgctg gcttccgagc gggccgtggc agttctggca agcgaccatt gcagcaatct
     6421 agaggtctgg actgccctgc agcccccaga gctgatcgaa cgcctccagg ccctggctca
     6481 gcgctatcac cgccagcagg atgagcgccc tttgccggcc ctggaaccac gcgtctatct
     6541 gtctttgacg atcagtgacg gagataacct gcaatacgtg cagcatcgta tgctgcatct
     6601 gtggcgcgat tcggcccgtg gcagattccc acttggctgg acgatcgccc cagcgctcct
     6661 ggaggccgct cccgccctgg cagcctatta cctggagaca gccagccccc aggatgagct
     6721 gatcgccggc cccagcggcg ccgcctacat gttcccatcg agctggccag ctgcccagcg
     6781 gccagccttc ctggagcata gccagcgcct gctctcaggg atgggcctct ctctcctgca
     6841 ggtgctcgat ggcgactggc gtcagcggct gggcctgccg ctcccggcta gcatgcgcct
     6901 gtctaatgtt gaggagcagc acgcctttat cgaggccctc cggcccgctg ggctgcgcgg
     6961 cctgctcaca ggtgccagtg gcctcaccct aagctataag aaaatcatgg gcatgcccgt
     7021 ctatcacaat ctggggcttg tcagcagcag gtctcagctg gtgcacctcg tcaggggact
     7081 gcaagccacg catcgtcggc gcccgctctt cctccagctc tatgtgctgg cctggcgtct
     7141 gacgcccagc gccctctggg aggcattgca ggaactgcgt gagatcgctt atccggtgac
     7201 cccgggtcag cttctacagc tcattgccca acaaggagca gggggagcag aatcgtgatg
     7261 agtcctcaga gctactatta tccatcgcct ggttgctacc gcctggctcc tggcctgtgc
     7321 ctgctagaag agatagacgg gcggccaggg ggactggtta tcgcttccta cccgctgcgc
     7381 gtcttgcgcc ttggccagca agcctggcgg ttactcaacc tgtgtcgcca agagcgcagc
     7441 agcgaggagt tggttcgcct gagcggactt gcccccgcgc gggtggccac gctctgccag
     7501 cagctcagtg ccaaagggct attggaggcc ggccctccac taccaccgag gtcgtggcca
     7561 ggtgtctccg tgatcgtgcc cacctgcaac cgtgtccgcc agcttgagcg ctgcctgcag
     7621 gctatccttg agttgcgcta tccatcacag gcactggaaa tcatcgtggc cgatgatgct
     7681 tctcgcgatc agacgccgac tctgctcacc cgcttcgctc cagccttcgc cagtcgtgga
     7741 gtgagactgc attgtctgcg ccacgcccgg cgtcagggag ccgcaggcgc ccgcaacagt
     7801 ggcgccgccg ccgctcgcta cgatctcctg gccttcgtcg atgatgactg cgtggtaaca
     7861 ccagactggc tcaccaccct ggtaccactc ttcagcgaga ccagcgtggc cgccgtcggc
     7921 ggtcaaatcc gcgcctacga aaccggcagc gcgctggggc gttacgagga cgtccgttcc
     7981 tcgctcttca tgggcctgca gccccaggag gtgcgcttga gtgggccgct cacctacctt
     8041 cccaccgcct gcttactcgt gcgtcgcgaa gcctggcagg ctctggacgg ttttgctcct
     8101 ctgagctgcg gcgaggatgt tgatttctgc taccggctgc tcgctcaggg ctggcgcatc
     8161 cgctattggc ccgctcccga gagcgtggtc taccatgact accgcacccg ccttgggacg
     8221 ttcctgggga cgcgcgtgcg ctatgcctct gccgaggctg tgctccaaca acttcacccg
     8281 caacagcgcc ggatactgct gcttccacct tgggaagcaa gttttgccgc cctgaccctc
     8341 ggcagcctct ggcagctcat caccgctctc cccttgggta gccttttgtg gaccaggtca
     8401 cagggtcgac agcgcccaag caggacagga caggcgcgcc tctccctcct actggcgttt
     8461 ccggctctgc tgctgcctgg agccagcacc tggcgtcgcc atcaggctgc ccgacttgct
     8521 cgcctgccgc tgacagcctg gcaaatctgg caggccacgc tacgcagtta cctggcctac
     8581 acctaccacc tttgccgcca tctgacccgc tactatacgc tcgcgctact agccgcgggc
     8641 ctgctctttc cgccgctttt ggtgctggtc ggcctacttc aagccgtggt ggtaggcgtt
     8701 gattacgcgc gcctgcgtcc gcgcctgagt ctgcccgcat tcgctctcta ctctctgcta
     8761 gaggattgcg cctatgctct cggcctgctg ctcggctgct ggaggcagcg cacctggcgg
     8821 ccattgctag ccgtgataga atggaagaat gccacagtta cccaacttgc tcaaccatcc
     8881 gagccaggcc caccctctct accttcacgt gaccaacgat agcgttgaaa ggaggtgacg
     8941 cagacagacg atggcaggaa gactggaacc tgaatggggt tttattgaag ctcgcggtac
     9001 gcgactctac tacgaagtcg ctggacgcgg ccatccgctg gtcctgctcc acagcggctt
     9061 gctcgatcat cgctcctggg acccacagtt catggagttc gctcgctact acgaggttgt
     9121 acgctacgat cgacgcggct tcggtcgctc agcgatgggg aacgtcgagt actcggatgt
     9181 ccgcgatctt tacgatgtac tcaacgatct ggccatcgac cgtgcttact tgctgggcct
     9241 gtcaaggggc ggcagactag ctatcgattt cgccctgagc tatcccgaga tggtcgatgc
     9301 cttgattctg gccgccgcca acatcagcgg ctacgataac tatatgccag aaacgctgga
     9361 gcgcgggaag cgcatcgccg ccgctgcccg ggcgcaggat aaggagcagc tctatgagct
     9421 gtgggccact gatccggcga tgccgcacga ggacgaatat ccagaagcag cacagcgcta
     9481 ccgcgagcta ttgcgtgact acagctttgt gcactacctg aatccagtgc ccagcgagca
     9541 gctcaggcca cctgccctgc agcgtctggc agagatccgc cagccgacct tgattctgat
     9601 cggagacgca gactcgctgg agatgcaggc ccaggccgag ctacttgaag ccggcatcat
     9661 cgatgccagg aaggtggtta tccgcggcgc gcgccatatg ctgaacctcg aacgccccga
     9721 gaccttcaac cgagccgtgt tgacatttct acacagtctg gagcgtcgct aggtaggcag
     9781 gcggcagggc cagcagcgac acgacaacag gacagcgaga tgtcccccat cccgcactgc
     9841 atgctgcaca cacctgtcga gcaccacctc ctcagagcag tgctcgacct gacctgacct
     9901 gaccgccgct gcctcctggc aatgtttctt gaatgtcccg gccctcgcgc tcgtttcctc
     9961 accatcttct ggctactgta gcgctgttat taccttttgc ctatgcttgc cgcgggagag
    10021 cgctccccca catggccgcc gacgagcacg gggcctcatg cgaagcgggc aacggatcaa
    10081 cagaactacc agcgagcacg cactcggggt cacgcacttg caggctgcgc tggacgaacc
    10141 acttggtggc cgggcaaccg ccgtgacaga ggtcatagcg cacacagccg ccacaggagc
    10201 cagtccccat ttggcggtag aaggcaaaga ccggcgagtg ctgccagatt tcccgaaaac
    10261 tctgttcatg caggttgccg gcctcgaact cgggcgcctg gaaaaaggca cagggataga
    10321 cctcgccttc ggggcagata cagcaggtag ccgtcgccgc accacacata tccagccctg
    10381 ccagacgctc gccgaaagca ttcaaatgga aaaacgaatc ggcagtcagc acatcgggat
    10441 gctcccgcag ccaggccacg aagctgcgat actgctcacg cgtgggatgt agctcttccc
    10501 acacggcacg cccacgacca gatgggcgca ggcgcgtcac gcgcaatttg gcaccaaagt
    10561 tgccagcaat cgcatagaga cgatccaatt cggggaagct ataacgcgtc aggacactat
    10621 tgacggttaa actcaggccc tcggcagcca gacgctccag gccacgcatg gctcgccgaa
    10681 acgcaccttg cccacgaatg cgatcattgg tctcaggggt ggcaccgtcc agactgacct
    10741 gaaaataaat cggctccaga gcagccaggc gttgagcgcg ctcaggagtg atcagcgtgc
    10801 cattcgtgct gacgcacatc accaggtcac gctcgcggca gcgctccagc acgcgccaga
    10861 tgcccggata catgaacggc tcgccgcccc caaagtggat ctggaagacc tgcatctcgg
    10921 ccagctcatc gaccacggcc agggcctgct ctgtcgtcag ctcacgcgga cggcgcttcc
    10981 ccgaggagga gaggcaatgc acgcaggtca gattgcaggc agccgtgatc tcccaggtga
    11041 ccgtgattgg cgccgagagt ccccctcgta ggaaacgctg cgagccgctg gtctgtgcag
    11101 gcaagagcgg gtcaccagtc tcggcaaagg cagctctggc cagaggatga agagcgccag
    11161 gctccgcccg cctctggctt cccgtccact ggtggcgacg tccatgaaac tgtacgaatt
    11221 ggatcatgcc tttctgctcc ttcttctacg agtgctccgc ttggcggctc atggccgtcc
    11281 tggcccgccg tagcgaggtc gggtcctaga caactgtcgg gcattgctca ctctactcag
    11341 ccgccgtctc ctcaggcgcc aacccggcta tcacctcggc ctctaaatag ccgcgtttcc
    11401 gcagctcatc gagcgccgcc aagaggcgag ccctggctcc tgcggaggag acttgctgtt
    11461 caatggccgc actgagatcg cctgcaaact gctctcgcgc taagaggagc agatcaatca
    11521 gacgcggaga attgacgaat gagagcattc ctcgatcagc acagccatac ttatagagga
    11581 tgccgccaaa ggcctcgcgc cgaatcttga cctccggtag cagacgatag cggtagcccg
    11641 ccgcatgtgc tgtcatggtc atagcagtcc ttctttctac ctggtggtca ggcagatagg
    11701 cagcctgtct actgcgaagg caggggcctg gtgaccccta gcgtcccaac tctaaagcca
    11761 gctcgcttac tgcgcgagcg gacgcagcga gaacggtata cagaggaagc atactcagag
    11821 atctatccca gagtcaaggt caccagcctg aagcgttaga tactgtaggg gagagagggc
    11881 aagaccccgt ccacagctcc cccttgttag ctagaacact tggcagacaa gacaccgcgt
    11941 gctacacttg cctggatctt acctacagca agcaagatcg atagaagcga cacaacaggc
    12001 aagagagcac aaaaaactgt tccgagacag acgcaaggat cgaacgtcat gaatattaat
    12061 tcggccatac gccgtctcaa ccagatcttc atcgtgctct tcctagggct gagtcttggg
    12121 cttgtctact ggcaagtggc ggtcgcgcaa caggtcactg cgaatattca taatattcgc
    12181 cagtgcatga aagaccatgc cccggtacgt ggcaagatct atgaccgcaa cggggtactg
    12241 ctggccgagt ccaagccgat tgatcccaac aaccctttta aaggggtcct ttgcggctac
    12301 cagcgccatt actatctgga tggcatgccc tcgctcgcca atttgattgg cttctatatc
    12361 agtcccctct ttaactcgac aggaatcgag cacgcttttg acgactattt gagcgggcgg
    12421 gccggtttaa cggaactgac caacaacctt aacctgatcc tccaccgccc tcccgtgggt
    12481 gataatatct acctgacgat cgatacgcgc attcagaaga ttgtcgagcg cgattttgac
    12541 aagtatgcgc caagctacaa cccacccgtg ccagaactta agatcgataa cgataacgtt
    12601 tttgccacca accgtggcgc ggtgatcgtc accgatcctc atacggggga gattctggcc
    12661 atggtgacgc gtccgacata cgatagcaat cgcgtggccg ctggtgacct ggcatactat
    12721 aaccagctct tgcgcaatcc agatgcgccg ctactagagc gtcccgtcca gggtcagtat
    12781 gtgcctggtt cggtctacaa agcgatgacc ttgctggcag cgctggacac cggcacctat
    12841 cagttgaata cgccttcgtt tgactaccag catgcagtcg gaccggtgac gatcggtggc
    12901 gaggtcttcg cccctcagat gagcaacatc taccacgcca gctatgcgcg cacgttcccg
    12961 gtgagtatga atatgggttt ctctcactcg gataacgtga tgttcgcctt tatcggctct
    13021 aagctggggg cagatacctg gctggaatac aataaacgct tctatgtagg ccaggatatc
    13081 cccttcgact taccagttgc gcggagctca gtccttccac ctaacgggca actaccccca
    13141 aatctgctgg ccgagaactc ttttggtcag ggcgtggatc ttgtgacgcc gatgctcatt
    13201 tcattgattg atgacgcaat cgccaacaac ggtgagctga tgcgcccgat gctggtctca
    13261 cgcattcagg cgccagatgg caccatcatc aagtcgatgt cgcctcagac gctgagcacg
    13321 ccgatttcag agcagacagc cgcctccgcc cgtgaagcaa tgtacggtgt ggtgcgctgc
    13381 ggctcgggat cggtgattcc cgaactctat aactccccct ggaatattat cgcgaagacc
    13441 ggcaccggtc aggtcggtgg cggcaaggcc gcccacggct ggctgatgac ccaggctcct
    13501 taccaggacc cgcgcttgac tattgtaagc atgcgcgaga atgctggcga gggcgccaac
    13561 gtcaatggtc cagctgtggc agcgatgtat aacgacattt acaccagcgt gttgaaactg
    13621 cagcaaccgg cccccgccga ttcctcggtg ccgtactgca cacagcatgg cctgtggacc
    13681 tagtcatggc ttagatctta ccggcttcag gctctgcgcc tggcaggatg ctgatcctcc
    13741 tgaccagacg tggagcctct ttctttatcg accgaggatt ctctccgggc agaccccgcc
    13801 tggcttgtct gcctggcctt gtcagcctct cccagggatg gtatccttga agaagacagg
    13861 catgaggcaa ccgggaagcc ttagcgaggc cgttacctgg tacctcgcca ggcgagtcca
    13921 agaaggagca atagcaggta tggagtccct ctttgagcac aatcggtact tcccagaagc
    13981 agaagaacag tctgcctaca gtcagacaga gccagtgctg ccctcctcgc tggtggatga
    14041 gcagacgaca ggagccgagg acgaggagga gtttgaagag gagctgatta tcgaggactt
    14101 caccatcgat ggcatctgcg gagtctacta gcccggcggc tgaagggcgc tttgccgatc
    14161 gcgtcgttct gatcaccggt gcagcgcgcg gccagggtcg tgcgcatgct ctggcttttg
    14221 cccgcgaagg ggcacgcctg gtgatctgcg atgcctgccg ccaataccgc accgtccctt
    14281 atccgctggc cagccccgag gagctgacag ctgtggccag ggagattgag gccctggaac
    14341 gcccggtgat cgccgctcag gtggatgtaa ccgacctccc ggctatggag gccctggctg
    14401 aacgcacccg ccgcgagctg ggaccgatcg atgtggtggt ggccaacgcc ggcctctact
    14461 cctttgctct gagctggcag atgaccgaag agcagtggga cgagaccatc aacgtcgatc
    14521 tcaaaggcgt ctggatcacc tgcaaggtgg ctattcctca gatgctggag cggcgccagg
    14581 ggaaaattat ctgcatcggc tctacggcca gcttcagagg catggatcac ctggcccact
    14641 acgtggccgc gaagcacggg gtgatcggcc tggtgaagac gctggctcgc gagctggccc
    14701 cctacaatat taatgtcaat gccgtctgcc ccaccagcgt ggctaccacg atgtgtctca
    14761 accaggccct ctatgacctc ttcgccggcg gccccggccc tcaagctacc tacgagcata
    14821 tggtgagcca gatgaatcga atgaatcttt tcagcgatcg caacctgctg ccacccgagg
    14881 acgtgagcgc cgttgtgctc tggctggcct ccgacgaggc acgacacttg acaggctgcg
    14941 ctctgcccgt tgacgccggc tatttgaccc gttagagcga tcccctcttt cttttgttcc
    15001 tccttttttt ctttagcgac ctgtttcggc ttaaaggttt ctctcatctc gacttgacaa
    15061 gcttaacaag agagatcggc aaatctactt tctcgccagt cctacaggag atcacttcat
    15121 cttccgactt tatgcattga caaatataga gatgtatatt accatagcaa tgtatccgcg
    15181 gagaggcagg tagtagaaag tcattttcca ggttatcagg cagtctggtc tgcggtattg
    15241 ccacaactgg aaaggaagcg ttatgaagct agcagaagca ctcgtgctac gctccgatat
    15301 gaaaaagcag ctcgcccaac tgcgtaatcg cctcaagagg agcgcaatcg ttcaggaagg
    15361 ggatcaaccg cccgagaacc ccgagatttt gttgcgtgag gtcgaggagc ttctcaggca
    15421 gtatcgccag ctcatggtcc gtatcaatcg gactaacctg gaaagcaggc taccagatgg
    15481 ccggaccctg accgaggctc tggccgagcg agatgttctt gatctgcatc atagcattat
    15541 cagcgatctg gcagctgcag ccagcgagcg tttcgatcgc tatggacggg cggagatccg
    15601 ccgcctgcca acggtggagg ccgccgccct ctaccggcag cttgatcgca tcgcccgtca
    15661 gcgccgcgag ctggatacac ttatccaggc cacaaactgg gccaccgatc tgcaggagtg
    15721 agcgagagcg tctagccagc atccttccta atcgcggcag gtgcctctag cgttggcgag
    15781 agacgctcag aaggaaatgc tgcgtcttga gcgcggcgcg caggatggct tgctttgcaa
    15841 gctggtagcg agcgaatttc gacagcgaac acaaaggcgg caacggcctg aagcgttgca
    15901 tccccttgtg gaagccccaa ggcaggctct tgtgcactgt cacgcccgga ccgcgcacag
    15961 gggcaccgcg aacagcacag tgcgcactac catgtgttgg gtcgaagttc gctggcgagg
    16021 gcgggtaagg ggattcctgc acgccgcgcc ggctttctaa gcctggcaag gcaaagcacc
    16081 gctacgcaag ccaggtcgcc aggtcgtcgg gctggcgctt cccttcgggc gtgagcagca
    16141 cgagatggtg ccagggatca cgtacagcaa tgatctcacc gtggcgctcg atcggccaac
    16201 gttccctttc caggcgctca gctactctct cgatctccgc ccgcgtgggc agctcgatag
    16261 agaagaggcg caagccaagg ctaccttcag gtggtgcaga cgcaccacgg ctctcccagg
    16321 tgttcaggcc aaggtgatga tgatagccgc ccgccgagac gaagagcgct cctggcatgc
    16381 gcgcggtaat cgcaaaaccg aggagttgat gatagaagtg ctccgcctgg cgcaggtcag
    16441 ccacccgcag gtggacatga ccaatggtgg tgcccgccgg cagtcctctc cagggctgcc
    16501 tctctaactc tgctcgccct tcagtaagca tggccctcac atcaatggga tcagtggcca
    16561 tcgccacctg cccgctctgc caacgccagg tttcgcgagg acgatcacga tagatttcga
    16621 taccgttgcc ttcaggatcg gagagataga gcgcctcact gacaaggtgg tctgcatagc
    16681 cccccagggg gtaacgactg gccgctagtt gagccagcga gcgcgccaag gcgggtcgtg
    16741 aggggaccag aatagcgaag tggtagaggc cagtgctcca ggacggcggt cgcggggcct
    16801 cagcctgctc aatcaagagc agcagcggct gcttcttcat gattggttct tctgtctgac
    16861 ccgccagatc agccgctcct agccagatct gccgctcact tcggtcaatc agctgaaaac
    16921 caaggacctc ttcgtagaaa cgccgttccc gctccagatc ggctaccgtc agggcaacca
    16981 ggccgatgtg cgtggtggcg ggcagcatat ctgctgcatt gataggggtg ttctcttgct
    17041 gctctttcat cgctgctgca cggccctaag ctgctgcgcc gtgccctcct ttccttgctg
    17101 accgactaca ctactacacc cgctgtctac acaggggcca tcatcagcgt gtagaaaggg
    17161 tccttcccta cgctcttcct catggctgcc ctcctcttct cccttactta caaaaagtaa
    17221 gagaggaaca tgacagtata caacatggaa gggctgggcg caaggctagc agatcgagct
    17281 tatcacgggg ttgaccgccg ggtttgttgt gggtagacaa agagcggcca ttttattgca
    17341 ccagagcgac attgtagaac aagcatcctt tccagtatac ttatggatga tcgtatttgt
    17401 cgctgacttg ggcagccagt ccgggcgccg cgtcggcccg gggctctcag cgttcgcaag
    17461 agaagagagg acttcaacga tgaaaaccag aaccgccgtc atctacgaac ctggtacccc
    17521 catccgcgtt gaagagatcg aacttgacac gcccaaagag caggaagtgc tcgtacgcat
    17581 ggtagccgcc ggcgtctgcc attccgatta ccacgtagtc aaaggagacc tgccagccta
    17641 cttaccaatg gctctcggac atgaaggggc tggcatcgtc gagcaggtcg gtcctggcgt
    17701 cagcgacctg aagccaggcg accacgttct catgagcttc attcccgcct gtggcagctg
    17761 tccctcctgt gtttccgggc acagcaacct ctgcgacaag ggggcgggca cgctgagcgg
    17821 tcctttgctg gacggcacct ttcgcatgca tggcgaaaaa ggcgatattg gccaattttg
    17881 ctacatcggt agcttcagcg aatacaccgt cgtgcctgcc atgtcggtgg tcaagatcgc
    17941 cgattactat cccctgaacc gggccgtgct cgtgagctgt ggcgtcccca ccggggtcgg
    18001 ctctgtcatc catcgggccc gcgtcacccc cggcagcacc gttatggtca tcggctgcgg
    18061 cggcatcggc atgaacattg tgcagggagc agccatcgcc ggggcgcgca tgatcatcgc
    18121 cgttgacatc catgatttca agctggagaa ggccaaagag ttcggcgcca cccacactat
    18181 caactccagc caggaagatc ccgtcaataa ggccctggaa ttgacctggg gcgtcggcgt
    18241 cgactatgcc ttcgaagcga tcgccacacc ggagaccatt gcccaggctt tcaacgccac
    18301 cgccaagaat gggaccgtgg tagtggctgg cctctcgcct ttcctggccc aggccattcc
    18361 catctctccg ctcaatctgg tcctctacca gaagaccctc atgggtaccc tcttcggcga
    18421 ttcgcagccg cgcaacgaca ttcctatgct gctgcgcatg tacgagagcg gtaagatcaa
    18481 gctcgatgaa ctggtgacgc gaacctacaa gctggaccag atcaatgaag cctacgacga
    18541 tctgctcgcc gggcggaaca ttcgaggcgt gatcgaattc agctagtccg cctttgctgg
    18601 cggcctcctg acccagctga ctcgcctgct gacagaagtt tacctggagg acaaaactgg
    18661 ctctgtatcc ttctcctcaa cgccagggag gatgcagagc cagccagcca gccagccagc
    18721 cagccagaac acgtgttctg cttctttcgc gtctattatc tctcctcttc ctttccggca
    18781 agagagaccc agcactcgcg cacggctcct cgcctgcccc gctctgtcag gtagctaact
    18841 accccgaaca gggttcagtc cgttgttagc tggctactag taaaaattcg caccgatcac
    18901 gtacaataac tccagtggag gcgtgattgt ctgtcccgac tgccgccgtc agagacgact
    18961 gccccgcgag cgggcagcgg gcgagagacg aggcagcaag caaaggagca aggaccatga
    19021 agcaccagga agctgtcatt gttgcagcgg ttcgcacacc cattggcatc ggcaagcctg
    19081 acagagggaa gctcaccggc atccgccccg acgagctggc cgccatgact ctggccgaag
    19141 ttgtacgccg ggccggcatc gagaaagggc ttgtcgagga cgtgatcatg ggctgcgtga
    19201 cccagatcgg agagcaaggc ctcaacatag ggcgagttgc tcccctcatc gccgattttc
    19261 ccgtcgagac ctgcggtgtc agtgtgaatc gcatgtgcgg ttccagcgag caggctattc
    19321 actttgccgc ccaggccatt atctccggca ctatcgacgt cgccattgcc gccggcgttg
    19381 agagcatgag ccgcgttcca atgggttcag acaaggccgg cgccagcttt agcgagaagc
    19441 tcatcgcgcg ctacccgctg gtaccccagg gcatttctgc cgagctggta gcagagaagt
    19501 ggcacatcac acgcgaagag gccgacgcct tctcgttaga gagccaccgc cgcgcagccg
    19561 ctgccacagc agaaggacgc ttcaagccgg agattatgcc gatcgaggtg accctgcccg
    19621 acggcagccg cgaagtcatg atcagcgacc agggtattcg ccccaatact tcgctggagc
    19681 aactcgctgc ccttaagacc gtctttaagc ccgatgggat cattaccgcg ggtaactcca
    19741 gccagatcag cgatggtgct gccgctgtcc tcttgatgtc cgacagcaag gcccaggagt
    19801 tgggactcaa acccctggcg cgcattgtgg cgatggccac agctggcgtc gatcctgtgc
    19861 tgatgctcag tggccccatt cccgccaccc agaaggtcct caagaaggcc gggctccgtc
    19921 tggccgatat cgatctcgta gaaatcaatg aggccttcgc ttctgtaccg ctggcctggg
    19981 cccgcgagct tgatcccgat atgagccgcg tcaatgtcaa cggcggagcc atcgccctgg
    20041 gtcatcccct gggcgctagc ggcgctcgcc tgatgacaac gctcgtccac gagctgcagc
    20101 gtcgcggcgg acgctacggt ctgcagacca tgtgtatcgg tcacggcatg gcgacagcca
    20161 cgatcattga aaggatctag cctatgctcg atttcgcccc caccgaagag caggaggaga
    20221 ttcgcaagct cgctcagagc atcgcccttg atcatctgcg gccccatgct cgcaaggccg
    20281 agatagaaca tgacagccct aatgagctgt ggctgactct ggcccaaact ggcctgacga
    20341 ctcccttccc cgaggaatat ggcggatctg gccccctgga agctgtgacc tatagcctga
    20401 tcgccgagga gctggcctac ggcgacccgg ggctggcgct ccatattgtc ggttccctga
    20461 tgggaccgct cacggtctgg ctggccggca gtcgcgagca acaggagcgc tacctgcccc
    20521 cctttggcaa ttctaccagc ggctacttgc agcgtggcag cctggctttc gccgagcgca
    20581 ccggtggcta taccctggca gatatccact tgcaagcgcg tcgtaccagt gatggctatc
    20641 tccttaacgg cactaagcgc gacgttgtgc atggccagcg cgctgatctg cgcgtggtcc
    20701 tggcacgcct agccgagcat gagagcgagg gtcctgaggg caaactctgc gccttcgtgc
    20761 tccccggcac tcaggaggga ctgagcattc aggccgaaga ggagaagctg ggattgcagg
    20821 ctgctcccag cgccacctat ctcttcaacc aggttcagct gccagccgag gcgctgcttg
    20881 gggagacagg cagtagcggc gttgtgcgcg ctgcgacact ctatcagatc ctgcgcgccg
    20941 ctatcgcctg cggcaccgcg cgcgccgcct tcgaatattg ctcggcctac gctcgcgaac
    21001 gcatcgcctt cgggcgcccg attgtctctt accagggcat cgccttcatc atcgccgaga
    21061 tggccatgaa gctcgatgcc gctcgcctcc ttacctggcg tgccgcttgc tcgtgggatc
    21121 gcggcgaggc cgatgagacg ctgatccacg aggcagaaag cgcccaacgg caggctgtca
    21181 aaatggctca ggcggctaca acggatgcga ttcaagtcat gggtggagct ggctttatgc
    21241 aggaccaccc cgccgaaatg tggatgcgtg atgctgccgc gatggagtga tgccccatga
    21301 tcgatttttc tctgagtaaa gcgcaacagg cggcccgtga ctacgcccac caggtggccc
    21361 tggaggagat gcgtccgctc tcgctggaat gcgaccgcac cgaagagatt cctgagtcgt
    21421 ttttctggaa tatgcagcgt cgctactggg gcggcgccgc agctcaggca cgggcctacg
    21481 gccaggacga gggggtcaaa caggagaacc tgctatcggt gatctcccag gaagaaatgg
    21541 cctgggggga tgcggctctg gcaacggcca ttcctgggcc gggtctggcg gccccaccga
    21601 ttctgtcgca gggaacgccc gagcagcagg cccgcttcct gagcatttat atggacggcc
    21661 ag
//
