LOCUS       NZ_MCIF01000002        21128 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Thermogemmatispora tikiterensis strain T81 A4R35_scaffold00002,
            whole genome shotgun sequence.
ACCESSION   NZ_MCIF01000002
VERSION     NZ_MCIF01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Thermogemmatispora tikiterensis
  ORGANISM  Thermogemmatispora tikiterensis
            Bacteria; Bacillati; Chloroflexota; Ktedonobacteria;
            Thermogemmatisporales; Thermogemmatisporaceae; Thermogemmatispora.
REFERENCE   1  (bases 1 to 5961682)
  AUTHORS   Tomazini,A., Lal,S., Stott,M., Henrissat,B., Polikarpov,I.,
            Sparling,R. and Levin,D.B.
  TITLE     Analysis of Carbohydrate Active Enzymes in Thermogemmatispora T81
            Reveals Carbohydrate Degradation Ability
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5961682)
  AUTHORS   Tomazini,A., Lal,S., Stott,M., Henrissat,B., Polikarpov,I.,
            Sparling,R. and Levin,D.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2016) Faculty of Agricultural and Food Sciences,
            University of Manitoba, 66 Chancellor's Circle, Winnipeg, Manitoba
            R3T 5V6, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-MAY-2011
            Assembly Method                   :: Newbler v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 25.9x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003268475.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 03:19:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,660
            CDSs (total)                      :: 4,607
            Genes (coding)                    :: 4,541
            CDSs (with protein)               :: 4,541
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 8 of 66
            Pseudo Genes (incomplete)         :: 56 of 66
            Pseudo Genes (internal stop)      :: 6 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            CRISPR Arrays                     :: 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:11:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1498246
            Orig. end                         :: 1519374
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MCIF01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21128
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="5"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..21128
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21128
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11128](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11128
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            318..1169
                     /locus_tag="A4R35_RS06085"
                     /old_locus_tag="A4R35_06035"
     CDS             318..1169
                     /EC_number="3.5.1.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014625.5"
                     /locus_tag="A4R35_RS06085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06035"
                     /product="PIG-L deacetylase family protein"
                     /protein_id="WP_112427518.1"
                     /sec_met_domain="PIG-L (E-value: 7.4e-29, bitscore: 99.2,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADRNAPGAIEVIKVPDYEAMVIGAHPDDADFAAAGTSALWARQG
                     KKIVWVIMTDGTEGTEVPSMVDRELMMVREQEQRLACEVLGVQAVEFLRFQDGHLVNND
                     ETRKAVVRLIRKYRPRIVITHDPTQHIFAPDPDEEPDETAYLNHPDHRATGNIVLDAIF
                     PAAVNPHAYRELLVEGFLPYQVHELYLFNSEHDNTYIDISETIELKARGLQCHVSQFGP
                     QANLLEGVKRWAAETAREAQQKKGLQMQYAEGFRRIKLYIPGKEKGNQEQPSETQQEQA
                     AH"
     gene            complement(1261..2196)
                     /locus_tag="A4R35_RS06090"
                     /old_locus_tag="A4R35_06040"
     CDS             complement(1261..2196)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015377.5"
                     /locus_tag="A4R35_RS06090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06040"
                     /product="C39 family peptidase"
                     /protein_id="WP_189361380.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.1e-05, bitscore:
                     23.3, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVLVLVLFAVALTVSLFGLYLSARPSLRGRKRHPAAAERLLRTP
                     NTPRLRARQSPIATVAQPRRRSASVLSLSVASERVYGPGSFPWREIPWKILLLGLVSLL
                     IVIFLGSQLFLSRGVGFVLPYLWTAEPAATMTSNAPASTAHYDATSHLVRINQLDPAQY
                     DSQAQYQIWAYSACSAAAMTVVINSYGYHYRIADILKVESALHEITPDQGLLEEVGIAR
                     TAARFHFNTLWGHNLSLDQMLAIANSGRPVIVSFPPDRFPGGHLLVVRGGNTSTVYLVD
                     SSRLNYTSMARSRFRQLWGGFYAVLTPQAS"
     misc_feature    complement(2509..2523)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.59"
                     /tool="antismash"
     gene            2598..4313
                     /locus_tag="A4R35_RS06095"
                     /old_locus_tag="A4R35_06045"
     CDS             2598..4313
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A4R35_RS06095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A4R35_06045"
                     /product="hypothetical protein"
                     /protein_id="WP_112427522.1"
                     /transl_table=11
                     /translation="MAILAGWLSKQVIPEAVIEERLLRMAAVLEQRGGERALVLQPGAG
                     LVTFADPLVGVATQQKTALLDWLPGQQTFVYRRPLTGWHPLYYVQDWPEPGDLLFASEI
                     KALLAAGVPCRLFLPALEAVRRYGFLPAPWTAFAGIEVVPAGSLLRWQQGRTLVSELHD
                     YRLAASASGGEEGEATPDAVKAALRELDEILCAQCRHLLPLPAAPLLVLTAGTGAAATS
                     AELLSWVAQQQQQESSPSLQIATLAFAGQRHAYTSAEALAERLKAGLLAIRGVDAPEYW
                     LATVSAIEAPCALTFPLALHQALHTAGVESNARLALLGLGARPLLAPPPSPSLAGQESV
                     ELFHRYSVLLHGRSSRPRFPITEGDQLWAPEVRRCLAESPSWEETRHARRLARKTLALR
                     QPWQRWRYLDLHLRLPDLLLQTAQQLATFDRLILVAPFALPELAEALALLPPDYEHLIR
                     QAPPGNQLSPFLLASLVQSYRRSSAQAAWPATTLLSAPARSLAAELTGATALSSIGRDL
                     LAETLTPTALLQTGIFAPKSVEALQERSRGGGVPRELVLVFTTQLLCKLFGATL"
     gene            complement(4361..5989)
                     /locus_tag="A4R35_RS06100"
                     /old_locus_tag="A4R35_06050"
     CDS             complement(4361..5989)
                     /NRPS_PKS="Domain: AMP-binding (30-438). E-value: 5.5e-88.
                     Score: 287.2. Matches aSDomain:
                     nrpspksdomains_A4R35_RS06100_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019381415.1"
                     /locus_tag="A4R35_RS06100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06050"
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="WP_112427524.1"
                     /sec_met_domain="AMP-binding (E-value: 7.3e-102, bitscore:
                     339.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQGLMMNYPLTLQHAFNRATRLFYRKEVVTQTEEGLHRYTYADWG
                     KRTMQLANALKEAGVAQGDRVATFAWNTYRHLELYFAIPCYGAVLHTLNIRLFPEQLVY
                     IVNDAEDRVIFVDGDLVPALEQIADRLPTVKLYVVMGQPPYQPQKLHPVVDYEDFIATQ
                     PTTYAWPELDENTAAAMCYTSGTTGNPKGVVYSHRSIFLHSMGVGLADGPGLCEQDRVL
                     PVVPMFHANAWGFPHAGVMMGSSIVMPGRFLDPLRIAQLMEKERVTLAAGVPTIWIGLL
                     NVLEKEQFDFSSLRAIISGGSAVPQSLIEGLARKNITIIHAWGMTETSPLASLTRLRSY
                     QRDLPAEEQFRIRAKQGTVVPGVDFRVVNIESGEEVPWDGQTFGELQVRGPWVARAYYK
                     DASSGEKFVDGWLRTGDVAVVDADGMIQLVDRTKDLVKSGGEWISSVELENLIMGHPKV
                     LEACVVGVPHPKWSERPIACVVPKPEFAGQVSAEEILEFLRPKVAKWWLPDEVIFVEAI
                     PKTSVGKFDKKVLRAQYESRLASQA"
     aSDomain        complement(4676..5899)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R35_RS06100_AMP-binding.1"
                     /evalue="5.50E-88"
                     /label="A4R35_RS06100_AMP-binding.1"
                     /locus_tag="A4R35_RS06100"
                     /protein_end="438"
                     /protein_start="30"
                     /score="287.2"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="QTEEGLHRYTYADWGKRTMQLANALKEAGVAQGDRVATFAWNTYR
                     HLELYFAIPCYGAVLHTLNIRLFPEQLVYIVNDAEDRVIFVDGDLVPALEQIADRLPTV
                     KLYVVMGQPPYQPQKLHPVVDYEDFIATQPTTYAWPELDENTAAAMCYTSGTTGNPKGV
                     VYSHRSIFLHSMGVGLADGPGLCEQDRVLPVVPMFHANAWGFPHAGVMMGSSIVMPGRF
                     LDPLRIAQLMEKERVTLAAGVPTIWIGLLNVLEKEQFDFSSLRAIISGGSAVPQSLIEG
                     LARKNITIIHAWGMTETSPLASLTRLRSYQRDLPAEEQFRIRAKQGTVVPGVDFRVVNI
                     ESGEEVPWDGQTFGELQVRGPWVARAYYKDASSGEKFVDGWLRTGDVAVVDADGMIQLV
                     DRTKDLVKS"
     CDS_motif       complement(4787..4840)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS06100_0002"
                     /evalue="4.60E-04"
                     /label="NRPS-A_a6"
                     /locus_tag="A4R35_RS06100"
                     /protein_end="401"
                     /protein_start="383"
                     /score="12.7"
                     /tool="antismash"
                     /translation="GELQVRGPWVARAYYKDA"
     CDS_motif       complement(5399..5455)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS06100_0001"
                     /evalue="3.30E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="A4R35_RS06100"
                     /protein_end="197"
                     /protein_start="178"
                     /score="22.7"
                     /tool="antismash"
                     /translation="AMCYTSGTTGNPKGVVYSH"
     gene            6399..6605
                     /locus_tag="A4R35_RS06105"
                     /old_locus_tag="A4R35_06055"
     CDS             6399..6605
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911183.1"
                     /locus_tag="A4R35_RS06105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06055"
                     /product="KGG domain-containing protein"
                     /protein_id="WP_223258223.1"
                     /transl_table=11
                     /translation="MMISKTGRPRGLAALSPERRREIASKGGRTSQSRGTAHQWTAEEA
                     SAAGKKGSARYARRRAELQSQLS"
     gene            complement(6602..8026)
                     /locus_tag="A4R35_RS06110"
                     /old_locus_tag="A4R35_06060"
     CDS             complement(6602..8026)
                     /EC_number="6.3.2.-"
                     /GO_function="GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /locus_tag="A4R35_RS06110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06060"
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="WP_189361382.1"
                     /transl_table=11
                     /translation="MNYQEALAYLYSLSDYERGGSYVRDRNENLPRERCLLEAVGNPHQ
                     AYSCTLIAGTKGKGSTAAIIERVLREAGLRTGLYTQPDLHTFRERIRINGQLIGEEDVA
                     RLVPELRAAVERIEREGRFGPYITFEVATALAFLYFARQGVEHAVIEVGLGGRLDATNV
                     TMPLVSVITSIGFDHMEVLGDTLTKIATEKAGIIKPQGTVVTSAQASEALLAIAAVSER
                     QQAELIRVGPAEGDPAQEEVRAGRLPPLRYRYALEARTPESQRFTVETPTSVYRGLELS
                     LLGQHQLENATAAIATLEALRHKGIQWSEEALRAGLRSVYWSARLEVIGREPLVVVDGA
                     HTAESMQRLLEALRATFQFRRLVVVLSTLRNKDVAGMVQALAGVDAVVITTVANPRSLR
                     IEEIADQFRTHAPHVPLYHAETSRQAMDLALRLAEREDAVCAAGSLYLAGEVLRWAAAR
                     GNQQVAATIEGVDHAG"
     gene            complement(8048..8965)
                     /gene="folP"
                     /locus_tag="A4R35_RS06115"
                     /old_locus_tag="A4R35_06065"
     CDS             complement(8048..8965)
                     /EC_number="2.5.1.15"
                     /GO_function="GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="folP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922274.1"
                     /locus_tag="A4R35_RS06115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06065"
                     /product="dihydropteroate synthase"
                     /protein_id="WP_112427528.1"
                     /transl_table=11
                     /translation="MSVAAAATVPLPPTIWDRYHLEWGRRTYVMGIVNVTPDSFAGDGL
                     LPTGAATLSDPAVRDQVVQRAVAQAQQFVSEGATFIDVGGESTRPGASAISPAEEQERV
                     LPVIRALRAALPEDIIISIDTYRAEVARQALEAGANLINDIWGLRADPEMAALAAERGV
                     PVVLMANMRGFQKRDIVSDVLRFLAGSIEIALAAGVAWERIIIDPGIGFGTTPEENLTL
                     LRRLSELRSLGRPLLLGTSRKSTIGLVLGGLPPQERVEGTAATVALGIAQGADIVRVHD
                     VREMMRVVKMSDAIVRGAFSIPKS"
     gene            9477..9971
                     /locus_tag="A4R35_RS06120"
                     /old_locus_tag="A4R35_06070"
     CDS             9477..9971
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A4R35_RS06120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A4R35_06070"
                     /product="hypothetical protein"
                     /protein_id="WP_112427530.1"
                     /transl_table=11
                     /translation="MEDPITTRRRQQALSQSRRCRECEQEALGRCPDCHRSFCQEHFPK
                     QQHSPCAERQLRLAEIQVCYVCGVPVYPDQWSISRTSHFIDQYRCKGCGRYVCEELHTR
                     KKDEDVVIVREGLRGHRYQYTIRYCDLCAPLSYVGGLKGLARWVTLVGTVVALVFFHFH
                     P"
     misc_feature    9978..9989
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 19.77"
                     /tool="antismash"
     gene            10001..11128
                     /locus_tag="A4R35_RS06125"
                     /old_locus_tag="A4R35_06075"
     CDS             10001..11128
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012568.5"
                     /locus_tag="A4R35_RS06125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06075"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_112427532.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 3.6e-56, bitscore:
                     188.4, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAISTTVQATLQRYREEIQAALREAVARATASTGAEASGLLQDYY
                     GQMRYHLGWVDAQLHPINAQTGKLLRPTLLLLSYEAAGAWGQLPPGDEPRHYLQRALPA
                     AAAIELTHNFTLIHDDIVDEDSERHHRPTVWKLWGVPHAINAGDGMFALSRLALWGLLE
                     IGVDPALAARLAALLDRTCLTIAEGQYLDMLFERRMDVSVEAYIEMIRRKTAALMACAA
                     EMGALLGTQDAETVERLRHFGEAIGLAFQVRDDVLGVWASLDELGKNPAGDIYRRKKSL
                     PILHALAQAQPADRERLQILYQQSAPLSREEVDEILAIFARTETRSYCQAFLAEQCRRA
                     RAALDAVPASSTSLAKRARSDLEAFVAFIEAASQN"
     gene            complement(11304..12812)
                     /locus_tag="A4R35_RS06130"
                     /old_locus_tag="A4R35_06080"
     CDS             complement(11304..12812)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1173: WD-40
                     repeat-containing protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF020396.5"
                     /locus_tag="A4R35_RS06130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06080"
                     /product="EsaB/YukD family protein"
                     /protein_id="WP_112427534.1"
                     /transl_table=11
                     /translation="MLEVIIEDSLSGLRYPVEVSGEAPLWSLVPALVKVLKLPLEDSTG
                     NRLSYRLSLAESGRELPRELSLEEAGVKAGTSLLLRPYVVGPAASGRGPVEDGLHDAVT
                     LVDATALPAVGVLGSPAADGGQQAAGRKRSRRLVLSLLGLTVVGVAGASAGYAAYRSGV
                     LGGWLTMQQRQGRPATTAVPKQTPTPTAAPLPTTARASLVFTQHRQTVRSLAWSPDGKL
                     LASGSLDGQLLIWNTQGQVQHTFTVNGAVRTLAWSPDGTQLALGAGSAVQFLDPYNGTP
                     LTQPLRQHTATVTAVAWAPHGQARLVSTSLDKLAIVWDGKLWRPLQVFRRHTSPLEAAS
                     WAADGQTIATASTGGLVRVWRADSDQELHGYYQDGQIALRTLAFAPTGMLLAVGGDDGL
                     IRLWNGLTCQRQQAGAFGTQCVDGPQHLHLHTQPLRTLAWSPDGRLLASAGNDGLLAVW
                     YPTHSQMPLFTVRLGAPILSLQWSADGQTIATAAGNRVTLWQLS"
     gene            13198..15063
                     /locus_tag="A4R35_RS06135"
                     /old_locus_tag="A4R35_06085"
     CDS             13198..15063
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016289.5"
                     /locus_tag="A4R35_RS06135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06085"
                     /product="M28 family peptidase"
                     /protein_id="WP_112427536.1"
                     /transl_table=11
                     /translation="MPLPGKTCLWQPTSRKATLVQRLLLSLLLISSLWPGYFKSPSASA
                     SGRSSGVASFPTVDGNYIYQQLFYMATHFLRREAGYDRNLPPTVNGHDEFAAYWSAEML
                     RNLSGFDPQVQRDPFAIRGWLNRPALVPAFNVEVSVPGIKQPEQIIIIGCHYDGMAFST
                     QSAYDDASGCAIELGIARGLASYWRAHQLYPARTLRFVLFDAEEQGLYGSFHAVNETMN
                     ADLGNIVAMFNEEQSGIAYPVRYLGQAANPVFPLYVDLSPLTSTKLYPQQNALSAEQRQ
                     RISQLRNLMDQAVPAVFAELYEEGFQMLSYHGKQQQDIFEPIFTPDQQQDVIREDDRLG
                     SSDQVPFTLAGIACATLVGNSSYYSTAATPGYPFDQPEDTIQLMNTFADGSSQQSQALT
                     LALTLPAMLTTWLLNQPAIVGAAPADGRPVTAINDLGRARPGLPLTVEAQAAFLPGSSL
                     PVGRFSYRWDFGDGESAHGQRVSHVYHRAGEYQLRLTVTAANGSQRLISQTLVVRQQSP
                     SYPNPYVRFLNNGMPPGNPGITLPTPNNRLSDRVTTVAQATAITTVPSAGTAHPLALAL
                     GVAIGLALLFVILIGVLLFISRSQRNDRQNASAHVRREHGPGGSS"
     gene            15065..16711
                     /locus_tag="A4R35_RS06140"
                     /old_locus_tag="A4R35_06090"
     CDS             15065..16711
                     /EC_number="3.6.1.1"
                     /GO_function="GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /locus_tag="A4R35_RS06140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06090"
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="WP_112427539.1"
                     /transl_table=11
                     /translation="MAQPIYVIGHKQSDLDSIASAYAYARLLQAQGEEAIPARHGSIKP
                     EVRFVLERYGVEPPEALEDVYLQVRDVMRRGVISIHVDQPLLEAGRLLQEHNRRAMPVI
                     DDEGRVRGIIAIEDLAKLFFKDLDPQAVNRVPLERDNLVRVLHGRVLVEGRRKLGNRIL
                     VGAMQAATMREYLEPGCLVVLGDREDAQLAAIEAGAAALVITGDLLVSERTLAAARKHG
                     VLVISTAYHTFTAVRLINLSMKTSEIMNRDFDSCRPEDLMSEIQPLLARHRSLPVLDDE
                     GRLVGYLSRTDMLNARPRRIVLVDHNERAQAVDGIEEAELLAIIDHHRIADVHTSKPIM
                     FRAEPVGCTGTIIVSLYHEAGVPISREAAGLLLAGLLYDTLILRSPTTTERDEQVAAEL
                     AEITGEDLEAYGQAIFAAAASDLNERSPEALLTADFKEYTVHGLRFAIGTVETATPSVI
                     EQRSAELLETMRQLSQERNYSVFLFMIVDIINMRCRLLIHGGEQAVATALGAPLGSDGH
                     TIEVEGLVSRKKQLVPLLGRIRSLLQERVRS"
     gene            17069..18142
                     /locus_tag="A4R35_RS06145"
                     /old_locus_tag="A4R35_06095"
     CDS             17069..18142
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024491.5"
                     /locus_tag="A4R35_RS06145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06095"
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="WP_189361384.1"
                     /transl_table=11
                     /translation="MRMKRYAERTASRWQKGLFPVLFCLLLLLSACNIEITAGGSTLLA
                     SPTAVLSNGAQQGLQVFVEPDAGEGVITNAINGAHRSVWLELYLLTNRNVINALEEAAN
                     RGLDVRVMLETHPYGGGSLSPTETMDRLSAAGVKVKATSPAFALTHEKGMIIDGTTAYI
                     MTCNLTNAALGVGSTKNREYGIIDTNPPDVKAVSDIFEADWNRTQVSLSNPNLVVSPIN
                     SRSVLESLIKGAQKSLLIEAEELIDDTIEQDLAAAAQRGVTVQVILPYQSSSSDSNHSG
                     IMAIKRGGAQVREDKQLYMHAKLMIVDGEKAFVGSENFSSQSLDHNRELGIVLSDQHIL
                     SVLQQTFSKDWNSSRSV"
     misc_feature    complement(18423..18436)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.99"
                     /tool="antismash"
     gene            18740..20836
                     /gene="ligA"
                     /locus_tag="A4R35_RS06150"
                     /old_locus_tag="A4R35_06100"
     CDS             18740..20836
                     /EC_number="6.5.1.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /gene="ligA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843724.1"
                     /locus_tag="A4R35_RS06150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_06100"
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="WP_112427543.1"
                     /transl_table=11
                     /translation="MADQSFEHEHEQTGVEPSQEASSRASESEELAARVAELRRRIEEA
                     NYQYYVLDNPTLTDAEYDQLMQELRRIEEEHPELQTPDSPTQRVGAAPLPEVRQHRHPV
                     PMLSLANARSEEELQAWHRRAQNILPNATFEYVCELKIDGLAMALTYEQGRLTVGATRG
                     DGLVGEDWTPNVRTIRSIPQRLRDANPPPRVEVRGEIYMTIESFEKLNASLTREKLFAN
                     PRNAAAGSLRQKDPRITAERNLDFFGYQIGYIEGRNLSTHWEALQLIAEWGFPVNPHNR
                     RARDLDEVLQYCREWEQKRFELPYEIDGVVIKINDLSQQQELGYVGREPRWAIAFKYPP
                     IQVATRLLDIRINIGRTGSVNPWAVLEPVNIRGVTVSRATLHNEQDILRKDLRVGDWVI
                     VQRAGEVIPQVVKPIVERRTGEERPYTPIERCPVCETSIMRLPDEAIAYCPNTDCPARL
                     LESVIHFASKGAMDLETIGEKQCEQLVKAGYIKTVADFYDLTREQLLKLEGVKEKSADN
                     MLKAIEASKKRPFWRLLFGLNIRYIGEKTAQLIAEHFGSMDRLLMASEEEIMAIPGVGP
                     KSAHSLYTWLQQEKNRQLIERLRQAGVNMASEERPGGPLAGQSFLFTGKLTHLTRSQAE
                     EAVRQLGGTVASNVTRSLTHLIVGEDPGSKLEKAQRAGVQIHDEQWFLDLLRQHGINIS
                     SSGT"
ORIGIN
        1 gggagtctgc tacagtccgg gctgctgctg cgctgggagg tggagccgct cgccctggtt
       61 ctgggtcaga cggcagaaga gcaggctctg ctgcgccgcg agctgccgcg ccgtgctgtg
      121 ggactggaga ctctggcagg ccgagcggtc tccgttgcag aagtgatggt cgctttcgaa
      181 caggccctgc tggctcgatg tttctctcct ccaacgccgc aaaagcgccc cacatctcct
      241 gctgagactt gacgaggcgc cgctctcgtg cctatactag gcaaactcca tgagtatagt
      301 agcagaggtc ctttactatg gcagatagga atgcaccagg agcaattgag gttatcaagg
      361 ttccagacta cgaagcgatg gtcatcgggg cccatcccga tgatgcagat tttgctgccg
      421 cgggaaccag cgccctctgg gctcgccagg ggaagaagat tgtctgggtt attatgaccg
      481 acggtacgga gggtaccgag gtacccagta tggtggatag ggagctgatg atggttcgcg
      541 agcaggagca gcgcctggcc tgcgaggtgc ttggggttca ggcagtggag tttctgcgct
      601 tccaagatgg ccatctggtg aataatgatg agacgcgcaa ggcggtggtg cgcttgattc
      661 gcaaataccg tccgcgtatt gtgatcaccc atgatccgac ccagcacatt tttgcccccg
      721 atcctgacga ggagccagat gagacggcct atctgaatca tcctgatcac cgtgccactg
      781 gcaatattgt gctcgatgcc atcttcccgg cggcggttaa tccccacgct taccgcgagc
      841 tgctggttga agggttcctt ccctatcagg ttcacgagct gtatcttttc aacagcgaac
      901 acgataacac ctatattgac atcagcgaga cgatcgaact gaaggccagg ggcctgcagt
      961 gccatgtcag tcagtttggt ccccaggcga atctgctgga gggtgtgaag cgttgggctg
     1021 ctgagacggc ccgcgaggcc cagcagaaaa agggtctgca gatgcagtat gccgagggct
     1081 tccggcgcat caagctctac atccccggca aggagaaagg gaaccaggaa cagccttcgg
     1141 aaacccaaca ggagcaggct gctcactaag caggcaactg ccaccaccgg gaagagccgt
     1201 tagcgaggaa ggcaggcgca gcgcgaaatg agtcggggaa gagctgacac ggcgagatta
     1261 tcaggaggct tgtggcgtca gcacggcgta gaagccgccc cagagctgcc ggaagcgtga
     1321 gcgtgccatt gaggtgtagt taaggcgcga tgagtcgacc aggtagaccg tgctcgtatt
     1381 acctccacgc accaccagca ggtgaccgcc aggaaaacga tcaggcggga agctaacaat
     1441 caccgggcgc ccgctgttag cgatggccag catctggtcg agcgagagat tgtgccccca
     1501 gagcgtgttg aagtggaagc gagcagcggt acgagcgata cccacctctt ccagcaagcc
     1561 ctggtcaggt gtaatctcat ggagagcaga ctcgaccttt aagatgtcgg cgatgcggta
     1621 gtgatagcca taggagttga tgaccaccgt catagcggcg gcggagcaag ccgaataagc
     1681 ccagatctga tattgggcct gggagtcata ctgcgcagga tcgagctgat tgatgcgcac
     1741 caggtgactt gtggcatcgt agtgagcagt agaggccggt gcgttcgagg tcatcgttgc
     1801 cgctggctca gcagtccaca gatagggaag gacaaagcct actccacggg agagaaagag
     1861 ctgcgagccg agaaagatga caatcagcaa cgaaaccagg ccgagaagga gaatcttcca
     1921 ggggatctca cgccagggga aagaaccagg gccatagaca cgctcagaag ccactgagag
     1981 cgagagtacg ctcgcgctgc gtcttcgcgg ttgcgccacg gttgcgatcg ggctttggcg
     2041 cgcgcgtaag cgaggtgtgt tcggagtgcg cagtaggcgc tctgcggcag cagggtggcg
     2101 tttccggcct ctcagcgatg ggcgcgcgga caggtagaga ccgaacagtg agacggtcaa
     2161 ggcgaccgca aagagcacga gcacgagcac aggcatcgat acaggctcct cagcatcgcc
     2221 agggtgccac gtctctggcc ggctgtcgcg cagcggagcg cgccattttc ctggcagtga
     2281 gtatgatgta gccaacggtc tggctagaag gaagtatagc atagcccacg ccagtcgggc
     2341 aaggcgtatc ctgagcaggg gacagggtgt cacatctttt ttagactagt gcctttctga
     2401 ctgaagacag ctcagaacac gtactctgat agaaagtcac cccccgcctc cgctagccgg
     2461 atcttccccc tcctttcccc ccgcgcgcac ctgggcggta ctctatgcta ttatcagaag
     2521 atgtgaaagc ttgagagtga gcgtgagcac ctgcagcaag cagttgtacc gcgtatcagg
     2581 caggagcgct tttgacaatg gccatcctgg caggctggtt aagcaagcaa gtcatacccg
     2641 aggcagtaat tgaggagcgg ctcctacgca tggccgcggt gctagagcag cgcggtggtg
     2701 agcgagcgct ggtgttgcaa cctggggcgg gtctggtgac cttcgccgat ccactggtgg
     2761 gagtcgctac tcagcaaaaa acggcgctcc tggattggct accgggtcag cagacttttg
     2821 tctaccgccg tccacttact ggctggcacc cgctctacta cgtgcaggac tggccagagc
     2881 cgggcgacct gctctttgcc agcgagatca aagccctgct ggccgcaggc gtcccgtgtc
     2941 gcctattcct gccagccctg gaggctgtgc ggcgctatgg gttcctgcca gcgccgtgga
     3001 cggcctttgc cggcattgag gtggttcctg ctggctcctt actgcgctgg cagcaggggc
     3061 gtacgcttgt cagcgagctg catgattacc gcctggcggc ctctgcttca gggggagaag
     3121 aaggggaagc aaccccagat gcggtcaagg cagccttgcg tgagctagat gagatcctct
     3181 gtgcacagtg tagacatttg ctgccactgc cagccgcccc cttgctggtg ttgaccgccg
     3241 ggacaggggc agcggccacg agcgcagaac tgctcagctg ggttgcccag cagcagcaac
     3301 aggagagcag cccctccctc caaatcgcca ccctggcctt tgctggtcag cgccatgctt
     3361 acacgagcgc ggaagcgcta gcagagcgtc tcaaggccgg tttgctggcc attcggggag
     3421 tcgacgcgcc agagtactgg ttagcaacgg tgagcgccat cgaagcgcct tgcgcgctga
     3481 cctttccgct ggctttgcat caagccctgc acacggcagg ggtcgaaagc aatgcgcgcc
     3541 tggccttgtt gggattggga gcacgcccct tgcttgctcc tccgccttcc ccctctctgg
     3601 caggacagga gagtgtagag ctgttccaca ggtatagtgt gctcctacac ggcagatcgt
     3661 ccaggcccag attccctatt accgagggag accagctatg ggcgccggaa gtcaggcgtt
     3721 gtctggccga gagtccaagc tgggaggaga cccgccatgc ccggcgcctg gcccgtaaga
     3781 cactggcgct gcgccagccc tggcagcgct ggcgttatct ggacctgcat ctacgtctgc
     3841 ccgacctgct tctgcagaca gcgcagcagc tggcaacctt tgaccggctg atcctggtgg
     3901 cgccgtttgc gctgccggag ctggcggaag cattggccct ccttccgcca gactacgagc
     3961 atctgatcag gcaggcccct cctgggaacc agctcagccc gtttttactc gcatctctgg
     4021 tccagagcta ccgcagatcg tcggcccagg cagcctggcc agcgacgacc ctcctcagtg
     4081 cgccggctcg ctcattggca gccgagctaa ccggcgcgac agccctgagc agcatcggtc
     4141 gcgacctgct cgccgagacc ctgactccga cagccctgct gcagaccggg attttcgccc
     4201 ccaagagcgt ggaggccctg caagagcgtt cccgtggcgg tggtgtgcca cgggaactgg
     4261 tactggtttt cacaacgcaa ctgctctgca aactcttcgg ggccacgctc tagcgccagg
     4321 cctggcgctg gctctcctcg caagcccagg cagtcctgga ctaggcctga ctggctaggc
     4381 gcgactcgta ctgggcacgc agcaccttct tatcgaactt gcccacgcta gtcttgggga
     4441 tggcttccac aaagatcact tcgtctggca gccaccactt ggcgaccttg gggcgcagga
     4501 actcaaggat ctcctcggca ctcacctggc cagcaaactc cggtttggga acaacgcagg
     4561 caatcgggcg ctcggaccat ttcggatgag gcacacctac cacacaggct tcgagtacct
     4621 tgggatgccc cataatcaga ttctccagct ccaccgaaga gatccactcg ccacccgact
     4681 tcaccaggtc cttggtgcgg tcaacgagct ggatcatgcc atcggcgtca acgacggcca
     4741 catcgcctgt gcgcagccag ccatcgacaa acttctcgcc ggaagacgca tctttatagt
     4801 aagctcgggc cacccacggg ccacgcacct gcagctcacc aaaggtctgc ccatcccagg
     4861 gcacttcttc gccgctctca atattgacca cgcggaagtc gacgccaggt acaaccgtgc
     4921 cctgcttagc gcgaatgcgg aactgctctt cggcaggcag gtcacgctga tagctgcgca
     4981 gacgtgtaag ggaggccagc ggcgaggttt cggtcatgcc ccaggcatgg ataatggtaa
     5041 tgttcttgcg cgccagaccc tcgatcagcg actgaggcac cgccgagccg ccactgatga
     5101 tggcacgcag agaggagaaa tcaaactgct ccttctccaa cacgttgagc aggccgatcc
     5161 agatagtggg cacgccagcg gcgagcgtca cgcgctcctt ctccatcagt tgggcaatgc
     5221 gcaggggatc aagaaaacgc cccggcatca caatgcttga tcccatcatg acacctgcat
     5281 gcggaaagcc ccaggcattg gcatggaaca tcggcacaac cggcagaaca cgatcctgct
     5341 cacagagacc ggggccatcg gccagaccca cacccatcga gtgcaggaag atcgagcgat
     5401 ggctgtagac cacacctttg gggttgcccg tagtaccgga ggtatagcac atggctgccg
     5461 cagtattctc gtctagctcg ggccaggcat aagtcgtcgg ctgcgtggca atgaagtcct
     5521 cgtagtccac cacgggatgc agcttctgag gttgataggg cggctgcccc atcaccacat
     5581 agagcttgac cgtcggcagc cgatcggcaa tctgctccag ggccggcacc aggtcaccat
     5641 cgacaaagat cacccggtct tcagcgtcgt tgacgatgta gaccagctgc tcgggaaaga
     5701 ggcggatatt gagggtgtgc aggacggccc catagcaggg gatggcaaaa tacagctcca
     5761 gatggcgata ggtattccag gcaaaagttg caacacggtc accctgggca acgcccgctt
     5821 ccttgagagc gttcgccagt tgcatagtgc gcttgcccca atcggcgtag gtgtaacgat
     5881 ggagtccttc ctccgtctgc gtcacgacct ctttgcgata gaacaggcgc gtggcccgat
     5941 tgaaggcgtg ttgcagcgtc agcgggtagt tcatcattaa gccttgcatg tgcgcatctc
     6001 ctgtcgtgga tcaagcgtta cgacatcgta ccggggcggg gcatgggccg gaaccgcaac
     6061 gccctgctct acagtattgt acgttgcaac cgccgccctc ttccagagag ccagcggctt
     6121 tttttcacaa ccagcaggcc gctccgccgg gtctggcctc taacaaaacg ccacaatctt
     6181 agagactgct gagaattcag acgatttttg taggtttgct tgcccaagga tttgagcttc
     6241 tgttatcctg gtatacagaa gagccaatat agcatgcctg gctgctgtca caacccggca
     6301 tagtggcggg gaagacgggg gcaggaggag cgagccggcc ccagcaacca gcggcaaccg
     6361 atacacaggt tagtctagat cgagcagagg agctgactat gatgataagc aagacggggc
     6421 gcccgcgtgg gctggccgcg ctcagccccg agcgtcgacg cgagatcgcc agcaaagggg
     6481 gccgtacctc gcaatcgcgc ggcacggccc atcagtggac agcagaagag gcctcagcgg
     6541 cgggcaagaa gggcagcgcc cgctatgcac gcaggcgcgc tgagctgcaa agccagctca
     6601 gctaacctgc gtggtcaacg ccttcgatgg tggccgccac ctgctggttg ccccgggccg
     6661 cagcccagcg cagcacttcg ccagccaggt agagcgagcc ggcagcacag acagcatctt
     6721 ctctttcggc aagccgcaag gccagatcca tcgcctgacg gctggtctcg gcgtgataga
     6781 ggggcacgtg aggagcgtgg gtcctgaact ggtcggcaat ttcctcgatg cgcagcgaac
     6841 ggggattggc aacggtggtg atgacaacag catcgacgcc agccagggcc tggaccatac
     6901 ccgcaacgtc cttgttacgc agggttgaga ggactacgac gagccgccgg aactggaagg
     6961 tggcccgtag cgcttcgagg aggcgctgca tcgattcagc ggtatgagcg ccatctacca
     7021 cgaccagcgg ctcccggcca ataacctcca ggcgcgccga ccagtagacc gagcgcaagc
     7081 ctgctcgcag ggcctcctcg ctccactgaa tgcctttgtg acgcaatgcc tccagggtgg
     7141 ctatggccgc cgtggcattc tcaagctggt gttggcccag caaggatagc tccagtccac
     7201 gatagacaga ggtgggagtc tcgacagtaa agcgctgact ctccggggtg cgcgcttcca
     7261 gtgcatagcg ataacgcagc ggcggaaggc gacctgcccg gacctcttct tgcgccggat
     7321 cgccttccgc cggccctacc cggatgagtt cggcctgttg cctctcgctc acggcggcaa
     7381 tggccagcag ggcttctgag gcttgagccg aggtgacgac tgtcccctgc ggcttgatga
     7441 taccagcctt ctcggtggct atcttggtga gcgtatcacc caggacctcc atgtgatcga
     7501 agccaatgga ggtaatgacc gagaccagcg gcatggtgac attggtggca tcgagccgtc
     7561 cccccagacc aacttcaatt acagcgtgct cgaccccctg acgtgcgaaa tagaggaagg
     7621 ccagggcggt cgctacttca aaggtgatat agggtccaaa gcgcccctct cgttcgatac
     7681 gctcgacagc ggcgcgcagc tcgggcacaa gcctggctac atcctcttca ccaatgagct
     7741 ggccgttgat gcgaattcgc tcacgaaagg tgtgtagatc cggctgcgta tagaggccag
     7801 tgcgcagacc cgcctcacgt aagacacgct cgatgatggc tgctgttgag cctttcccct
     7861 tggtacccgc tatgagagtg cagctgtagg cctggtgtgg attgccaacg gcctctagca
     7921 gacaacgctc gcgaggcaga ttctcgttgc gatcgcgcac gtaactgccg ccgcgttcat
     7981 agtcactgag gctgtagaga taggcaaggg cttcctgata attcacttcg atcgctacac
     8041 ctttcactca ggattttgga atagaaaagg ctccacgcac gatagcatcg ctcattttga
     8101 caacgcgcat catctcacgt acatcgtgaa cgcggacgat atcggctccc tgggcgatgc
     8161 caagagctac tgtggcggca gtgccttcga cacgctcttg gggcgggagg ccccccagca
     8221 ccaggccgat ggttgatttg cgcgacgttc caagcaggag agggcgcccg aggctgcgca
     8281 gttcgctcag gcgtcggagc aaggtcagat tctcttcagg cgtcgtaccg aatccgatgc
     8341 ctgggtcaat aatgatgcgc tcccaggcca ctcctgccgc gagagcgatc tcaatgctgc
     8401 ccgccaggaa gcgcaggaca tcgctgacga tatcgcgttt ctgaaagccg cgcatgttgg
     8461 ccataaggac cacggggaca ccgcgctcgg cagccagagc cgccatctca ggatcagcgc
     8521 gcaagcccca gatatcgttg atcaggttgg ccccggcctc cagcgcctgc cgcgcaacct
     8581 cagcccgata ggtgtcgatg gaaataatga tgtcctcggg caaggctgca cgcaaggccc
     8641 gaatgacggg caacacccgc tcttgttctt ctgcgggcga aatcgctgag gcgcctgggc
     8701 gtgtcgactc acctcctaca tcgataaagg tcgctccttc actcacaaac tgttgggcct
     8761 gcgcgacagc acgctgcact acctggtcgc gcacggcggg atcgcttaag gtggctgctc
     8821 ctgtgggaag caagccatcg ccggcaaagg agtcaggggt gacattaacg atgcccatca
     8881 cataggtgcg tcgaccccat tcgaggtgat agcgatccca gatagtcggc ggtagcggta
     8941 cggttgccgc tgctgctacg gacatggctg gctgccctca caacaataga gttcttgtct
     9001 ctggcccaag ctgcaaaggg tctcacaaga gtggtcgctt cagcctggac ttccagagtc
     9061 tctcggcgac aggacgccgt tgcgcgcgca tggtcgggtc gggtagggcc aagatcgatt
     9121 gtcctccgcc tgcttcgctc cgatgcctgg tcctggtgcc tatattatct tatcttctgg
     9181 cagcgcgagg ctgcaaacct gatcttgcct gcttgtctgc tatccagata cccgggtcga
     9241 gccactgcgg gcaagcggta ggcaccgctc cctcccctgg aagccggctc gctagaccat
     9301 cccggtcaca atgcggcaca ggtatgcccc tcatgctata ctacacagag agatagagat
     9361 attgctctcc tagtcaggcc agccggccaa cttgactaac tacccgtgat ccagcaagca
     9421 actcattaac cgcaccgctc tatcaactat aagaaaaggc agaggaagca gagacaatgg
     9481 aagatcccat tacgacgcgt cgacgccaac aggcgctgag tcagtcccgc cgctgccgag
     9541 aatgcgagca ggaggccctg gggcgctgtc ccgactgcca tcgcagcttc tgccaggagc
     9601 attttccgaa gcagcaacac tcgccctgcg ccgagcggca gctgcgcctg gctgagatcc
     9661 aggtctgcta cgtctgtggc gtcccggttt atccagatca gtggtcaatc tcccgtactt
     9721 cacatttcat cgatcaatac cgctgtaaag gctgtgggcg ctatgtttgc gaagagctac
     9781 ataccaggaa gaaagatgaa gatgtcgtta ttgttcgcga gggcttgcgc ggccatcgct
     9841 accagtacac gattcgctac tgtgatctct gtgcccctct ctcctacgtg ggtggcctca
     9901 aggggttagc gcgttgggtg acgctggtgg gaaccgttgt ggcccttgtc ttttttcact
     9961 ttcaccccta agatagggaa aaagttttct gcggagaagc atggccatct caactaccgt
    10021 tcaagcaacg ctccagcgtt accgcgagga gatccaggcc gcactcaggg aggctgtggc
    10081 acgcgccacg gcctctaccg gggcagaggc ttccgggctc ctgcaagatt actatggtca
    10141 gatgcgctat catctgggct gggtcgacgc ccagttgcat cccatcaacg ctcagacggg
    10201 caagctcctg cgccctacct tactgctcct ctcttacgaa gccgctggcg cctgggggca
    10261 gcttccccct ggggatgagc cacgtcacta cctccagcgg gccttgcccg ctgcggcagc
    10321 cattgagctg acccacaatt tcaccctcat ccatgacgac attgtggacg aagatagcga
    10381 gcgccaccac cgtcctacag tttggaagct gtggggtgtg cctcacgcca tcaatgccgg
    10441 cgatggcatg ttcgctctca gccgcctggc cctctggggc ctgctggaga tcggtgttga
    10501 tcccgccctc gctgcccgcc tggccgctct tctcgaccgc acttgtctga caatcgcaga
    10561 aggccagtat ctcgatatgc tcttcgagag acgtatggat gtctcggtcg aggcctacat
    10621 cgaaatgatt cgacgtaaaa ccgctgcctt gatggcctgc gctgccgaga tgggggcctt
    10681 gctgggcaca caggacgcag agaccgtgga gcggctgcgt catttcggtg aggctatcgg
    10741 cctggccttc caggtgcgcg acgacgtact gggcgtctgg gccagtctcg atgagctggg
    10801 caagaatccc gctggcgata tctaccgacg caaaaagagt ctgccgatcc tgcacgccct
    10861 ggcccaagcc caaccggctg atcgcgagcg gctacagatc ctgtaccagc aaagcgctcc
    10921 tctgagtcga gaggaggtgg acgagatcct agcgatcttt gctcgcacgg agacacgctc
    10981 ctactgccag gcttttctgg ccgagcaatg ccgccgagcg cgcgccgccc tggatgctgt
    11041 ccctgcttcg tctacctcac tcgccaagcg cgctcgaagc gacttagaag cctttgtggc
    11101 tttcattgaa gcagccagcc aaaactagcg ttcagcagca caggatcatc cagggccatc
    11161 ggcctcagca ggcggcagga ctggcaccgc cttcatcagc tccaggcaga gagcaagaga
    11221 tgagcagcgt cgcagctggc cagccagggc agcttctacg ggaaggctga gccagacctg
    11281 gtctggcgcg gtccggcagt cgtttaactc agctgccaga gcgtcacccg attccccgcc
    11341 gctgtggcaa tggtctgccc gtccgcggac cattgcaggg agagtatcgg agcgcccagg
    11401 cggacggtga agagcggcat ctgactgtgc gttggatacc agacggccag caggccatcg
    11461 ttgccggcgc tggccagcag cctgccatcg ggcgaccagg ccagcgtacg cagcggctgt
    11521 gtgtgcagat ggagatgctg aggtccgtcg acacattggg ttccgaaggc ccctgcctgt
    11581 tggcgctggc aagtcagccc gttccagagg cggatcaggc cgtcgtcgcc cccaaccgca
    11641 agcagcatgc ctgtgggagc gaacgctagc gtacgcagag ctatctgacc gtcctgatag
    11701 tagccgtgca gttcctggtc gctgtcagcc cgccagacgc gcaccagtcc cccagtggag
    11761 gctgtggcta tggtctggcc atccgcggcc catgaggcgg cttccagcgg actcgtatgg
    11821 cgcctgaaga cctgcaaagg gcgccagagc ttgccatccc agacaatggc cagcttatca
    11881 aggctggtcg agacaaggcg tgcctgacca tgcggcgccc aggctacggc ggtgacggtg
    11941 gccgtatgtt gccggagcgg ttgcgtcagt ggggtgccgt tgtagggatc gagaaactgc
    12001 actgctgagc cagctcccag agccagctgc gttccatcgg gtgaccaggc cagggtgcgg
    12061 acggctccgt tgacggtgaa ggtatgttga acctgacctt gtgtgttcca gatcagcagc
    12121 tgcccatcaa gcgaaccact ggccagcaat tttccatcgg gtgaccaggc cagcgagcgc
    12181 acggtctggc ggtgttgggt gaagaccagg ctggcccggg cggtagtcgg cagcggggcc
    12241 gccgttggcg tgggcgtctg cttcggtaca gcggtggtcg ctggcctgcc ctggcgttgc
    12301 tgcattgtca gccaccctcc caggacccca gagcggtagg cggcgtagcc agcgctcgct
    12361 cccgccactc ccactaccgt cagaccgagc aagctcagga caagacgccg tgagcgcttc
    12421 cttcctgccg cctgctgacc cccatctgca gctggtgagc ctagcactcc cactgctggc
    12481 agagccgtag catcaaccag cgtcacggca tcatgcagcc catcctccac cggcccgcgt
    12541 ccgctggcag ccggtcccac cacgtagggt cgcagcagca aggatgtccc tgccttgact
    12601 ccggcttcct ccaggctcag ctcacgcggc agctcccgtc cgctctctgc caggctcaaa
    12661 cggtagctca gccgattacc agtgctatcc tctagtggca gcttcaacac cttgaccagg
    12721 gccggcacca gcgaccagag cggtgcctcg cctgagacct ccactggata cctcagtcca
    12781 gagaggctgt cttcaatgat gacctctagc atgcttgtct cccctctttg aaggctcgcg
    12841 cctcacatcc acagcttctc tctgggagga atccacgaac gatcaagcgg cctatttttt
    12901 acagcttgcg ctcctccccc aaaatggcca ccaccaagaa agaggcggcg aaacgtcttt
    12961 tttagaaggg ggaagtgaga gccaggcgcc tttttctgcc acttgactgt caccatgctg
    13021 catacagtca tctgctacaa gccaggagag aaggaaggtc tgttcctgtc caccacggca
    13081 ccctcatatc cgaggtgcgc tttgcattgt cttagagcac tatggagttg agccgcccaa
    13141 gcccctggag ggggtctgct tgcaggtata caggcgactc tcatatggag gaagatcatg
    13201 ccattaccag gcaagacatg cttatggcag ccaacctcgc gcaaggcgac actcgttcaa
    13261 cggctcctcc tgtccctact gctgatcagc tcgctatggc ccggctactt caaatcgcca
    13321 tcggccagcg ccagcgggcg cagctctggg gtggccagtt ttcctactgt agacggcaac
    13381 tatatctacc agcagctttt ctatatggcc acccattttc tgcggcgcga agccggttac
    13441 gatcgcaatc tgccgcctac tgtcaacggc cacgacgagt ttgctgccta ctggagcgca
    13501 gaaatgctgc gcaacctgag tggcttcgac cctcaagttc agcgcgaccc ctttgccatc
    13561 cggggctggc ttaatcgccc ggcgctcgtg cctgcattca atgttgaggt cagcgtccct
    13621 ggcatcaaac agcctgagca gatcatcatc attggctgtc actacgatgg catggccttc
    13681 tcaacccagt cggcctacga cgacgccagc ggctgcgcca tcgaactggg catcgcccgc
    13741 gggttagcca gctactggcg tgcgcatcag ctctatccag ctcgcacgct acgctttgtc
    13801 ctcttcgatg ccgaggagca gggtctctat ggctcattcc atgccgtcaa cgagaccatg
    13861 aacgccgatc tggggaatat cgttgccatg tttaacgagg agcagagcgg catcgcctat
    13921 cctgtgcgct acctcggtca ggctgctaat cctgtttttc ccctctatgt cgatctctcc
    13981 cccttgacaa gcactaagct ctatccacag cagaacgcgc tctcagccga gcagcgccaa
    14041 cgtatcagcc aactccggaa tctgatggat caggctgttc ctgctgtctt tgccgagctt
    14101 tacgaagaag gctttcagat gctctcctat cacgggaaac agcaacaaga catctttgag
    14161 ccgatcttta cccctgatca gcagcaagac gtgatccgtg aggacgaccg gcttggcagc
    14221 agcgatcagg tgccgttcac gctggcaggg atcgcctgtg ccactctggt cggcaactcc
    14281 agttattaca gcacagccgc cacgcctggt tacccctttg accagccgga agacacgatt
    14341 cagctgatga acaccttcgc cgacggcagc tcacagcagt cacaggcttt gaccttagct
    14401 ctgaccctgc ccgccatgct cacgacctgg ctgctcaacc agcccgcgat cgtgggagcg
    14461 gcccccgctg atggtcgccc ggtcacagcc atcaatgatc tcggcagagc aagaccaggt
    14521 ctgccactga ccgttgaggc tcaggccgct ttcttgcctg gctccagctt accggtaggg
    14581 aggttcagct accgttggga tttcggcgac ggcgagagcg ctcatggtca gcgcgtcagc
    14641 cacgtctatc atcgggccgg cgagtaccag ctcagactta cagtgacggc tgccaatggc
    14701 tcccaacgcc tcatcagcca gacgcttgtt gtgaggcagc agtccccaag ttaccctaat
    14761 ccctacgtgc gtttcctcaa caacggcatg ccgcccggta accccggcat tactctgccc
    14821 actccgaata accggctcag cgaccgcgtc accactgtcg cccaggctac cgcaatcaca
    14881 acagtccctt cagctggcac agctcatcct ctggcgcttg ccctcggcgt ggctatcggc
    14941 ctggcactgc tttttgttat actgataggc gtactactgt tcatctctcg cagccaacgc
    15001 aacgaccggc agaacgccag tgctcacgtc aggagggagc acgggcctgg aggaagcagc
    15061 tagcatggca cagccaatct acgttattgg acataaacag tcagatctcg actcgatcgc
    15121 ctcggcctac gcttatgcgc gtctgctaca agctcagggg gaagaggcta ttcccgctcg
    15181 gcacggctct atcaaaccag aagtgcgttt tgtgttagag cgctacggag tggagccgcc
    15241 agaggccctg gaggacgtct atctgcaggt acgcgacgtc atgaggcgcg gcgtcatcag
    15301 tatccacgtt gatcagcccc tgctggaagc cgggcgcctg ctacaggagc acaaccggcg
    15361 cgccatgccc gtgattgacg acgagggccg cgttcgggga attatcgcca ttgaagacct
    15421 ggccaagctc tttttcaagg atctcgatcc acaagcggtc aaccgggtcc cgctggagcg
    15481 ggataacctg gtacgtgtcc ttcacggacg cgtgctcgtc gagggacggc gcaagctggg
    15541 caaccgcatc ctggtcggcg ccatgcaggc agccaccatg cgcgaatatc ttgagccggg
    15601 ttgtctggtt gtcctggggg accgcgagga tgcccaactg gctgccatcg aggctggagc
    15661 tgctgccctg gtcatcaccg gcgacttgct cgtctccgag cgcacgctgg ctgcagcgcg
    15721 caaacacggt gtgctcgtca ttagcaccgc ttatcacacc ttcaccgccg tccgcctcat
    15781 caatctgagc atgaagacca gcgagatcat gaaccgtgac ttcgactcct gcaggccgga
    15841 agacctgatg agcgagatac agccgctgct ggcccgccat cgttcgctgc cagtgttgga
    15901 cgatgaaggg cgcctggtcg gttatctctc gcggaccgac atgctcaatg cccgtccacg
    15961 tcgcattgtg cttgtcgatc acaatgaacg cgcgcaggct gtggatggca tcgaagaggc
    16021 agagctgctg gccattatcg accaccatcg cattgccgat gttcacacca gcaagcccat
    16081 catgttccgg gccgagccgg tcggctgtac gggcaccatt attgtcagtc tctatcacga
    16141 ggctggagtg cctatctcgc gcgaagccgc cggtctgctg ctcgccggac tgctctatga
    16201 tacactgatt ctacgttcac ccacgacgac ggagcgcgat gagcaggtag ctgccgagct
    16261 ggcggagatt acgggcgagg atctcgaagc ctatggccag gctatctttg ccgcggcggc
    16321 ctccgactta aacgagcgca gtcccgaggc tctgctcacc gccgatttca aggagtacac
    16381 cgttcatggt ctgcgcttcg ctatcggcac cgtcgagacc gccactccct cggtaatcga
    16441 gcagcgcagt gccgagctgc tggagaccat gcgccagctc tctcaggagc gtaactatag
    16501 cgtcttcctc ttcatgattg ttgacattat aaacatgcgc tgccgcttgc tgatccacgg
    16561 tggcgagcag gcggtggcca cagcgctcgg agcgccgctc gggtccgacg gccacaccat
    16621 cgaggtcgag gggctggtct cgcgcaagaa acaactggtt cccctgctgg gtcgtattcg
    16681 gagcctgctc caggagcgtg ttcgatcgta gcgccgccct ggccagagag gaagccgaag
    16741 ccaaccgcta cagaccagac cagaccagac cagaccggcg gaggaagaca aggcaggcag
    16801 cactgcgcag gcccatgctt tccggtctgg tctctctgtg atctgcgttt atccccaaca
    16861 ctggcaacgt cccggtcggc tcatcgccct gcgtcagtca ggccagcctt tgacaatgac
    16921 tgcttctgac gcgcctttgg ccccccagac gtgcttatct agagagtact gctcttgcac
    16981 tgctcccctc tcgtctgcag aagatatgat gaggctggag agaccattta tcgtctccat
    17041 tctactaacc gatcacggag tcgcttcgat gaggatgaaa aggtacgccg aacgcaccgc
    17101 ttctcgctgg cagaagggcc tcttccccgt gctcttctgc ctcctcttgc tcctgagcgc
    17161 ctgtaacatt gagattaccg ctggcgggag taccctgcta gcctcgccga ccgccgttct
    17221 cagcaatgga gcccagcaag ggctgcaagt ttttgtggaa ccagatgccg gcgaaggggt
    17281 catcaccaac gctatcaatg gggcccaccg ctcggtctgg ctggaactct acctgttaac
    17341 taaccgcaat gtcatcaacg cccttgagga ggcggccaac cgcggcctcg atgtgcgcgt
    17401 gatgctggag actcacccct acggcggcgg ctcgctctcg ccgaccgaaa cgatggaccg
    17461 cctgagcgcg gcgggggtca aagtcaaagc gaccagccca gctttcgctc tgacccacga
    17521 gaagggcatg attatcgatg gcaccacggc ctatatcatg acctgtaacc tgacaaacgc
    17581 cgctctcggc gtcggcagca ccaaaaaccg cgaatatggg atcatcgata ccaatccccc
    17641 agatgttaaa gccgtcagcg atatcttcga agccgattgg aaccgcacgc aggtctctct
    17701 cagcaatccg aacctcgtcg tcagccctat caactcgcgc agcgtccttg aatcgcttat
    17761 caaaggcgcg caaaaatcgc tgctcatcga agcagaggag ctgatcgacg acaccattga
    17821 gcaggacctg gctgccgcgg cccaacgcgg tgttacggtg caggtcatcc taccctacca
    17881 gagcagttcc tccgatagca accacagcgg tatcatggcc atcaaacgcg gcggagccca
    17941 ggtacgagag gataagcagc tctatatgca tgccaaactg atgatcgtcg acggtgagaa
    18001 ggcttttgtt ggctccgaga acttctcaag ccaatcgctt gatcacaatc gcgagctggg
    18061 tatcgtgctc tccgatcaac acattctcag cgtgctccag cagacctttt ccaaggactg
    18121 gaacagttcg cgcagcgtct gaaaaaccca taggcctcga ccacctcttg agccgcctgc
    18181 cgctaggcgg ttcagcaaga aggccggtcc tttctccaga ctgcccctga gacgacgggc
    18241 acaaacagcc tggaccgggc cggcacgagc gagccaaacc agccttgagc ggtatgcctg
    18301 gcgaactcgc atagcggagc agccagttcg ccaggcttct cgctcggcct ggcccagctt
    18361 gatagtacca ggctgacgtc gtccctgctt ctctctcccc gttgccacca tgtgatacga
    18421 taggaaagca tgttctgtca ctcccagtcc ttccgtagcg ctgacagcag cctaggccgg
    18481 accacaggac ctgagcgatt agcagaaccg acggggcaga tgggcaagca tccacacgct
    18541 cagccccagt ctcgcaacaa acctgatcac tcctgccaga gagcgttcct ctctggtcag
    18601 aagtgaccag tgactgacag acaggctgac tgacaagcag agatagaaca gacttttcct
    18661 ttcctggccc ttctcaaaag aacaagataa gtggggaggt atttcagtca gggagattct
    18721 ttttcgggga ggcaacctga tggccgatca gtcgtttgag cacgagcatg aacagaccgg
    18781 cgtcgagccg agccaggaag caagcagcag agcgagcgag agcgaggagc tagctgctcg
    18841 cgtggcagag ctgcgccgtc gcatagaaga ggccaactac cagtattacg ttctcgacaa
    18901 ccccacgctg accgacgcag aatatgatca attgatgcag gagctgcgac gcatcgagga
    18961 ggagcatcct gagctacaga cgcctgactc gccaacgcag cgcgttgggg cggcgccgct
    19021 ccctgaagtg cggcaacatc gccatcctgt ccccatgctg agtctggcca acgcgcgcag
    19081 cgaagaagag ctacaggcct ggcaccggcg cgcgcagaac atcctgccca acgccacctt
    19141 tgaatatgtc tgcgagctga agatcgacgg gctggctatg gctctgacct acgaacaggg
    19201 gaggctgacc gtgggtgcca ctcgaggcga cggtcttgtc ggcgaggatt ggacccccaa
    19261 cgtgcgcacc attcgcagca tccctcagcg cctgcgtgac gccaatccgc cgccgcgggt
    19321 cgaggtacgt ggcgagatct acatgaccat cgagagcttt gagaagctca acgcctcgct
    19381 tacccgcgag aaactctttg ccaacccgcg caacgccgcc gccggttcgc tccgtcagaa
    19441 agatccacgt atcaccgctg agcgcaacct cgacttcttc ggttaccaga tcggctatat
    19501 cgagggtcgc aatctgagca cgcactggga ggctctgcag ctgatcgccg aatggggctt
    19561 tccagtcaac ccccacaatc ggcgggcgcg ggaccttgac gaagtgctgc agtattgtcg
    19621 tgaatgggag cagaagcgtt tcgagttgcc ctacgaaatt gacggggttg tcatcaagat
    19681 caacgatctc tcccaacagc aggagctggg ttatgttgga cgagagccgc gctgggccat
    19741 cgccttcaaa tatcctccca tccaggtcgc cacacgcctg ttagacatcc gtatcaacat
    19801 cgggcgtact ggctcagtca atccctgggc tgtgctggag ccggtcaaca tccgcggcgt
    19861 gacggtctcg cgggccacct tacacaacga acaagacatc ctccgcaaag atctgcgcgt
    19921 cggcgactgg gtcattgtac agcgcgcggg cgaggtcatt ccccaggtag tcaagcccat
    19981 cgtcgagcgg cgcaccggtg aagagcgccc ctacaccccg atcgaacgct gccccgtctg
    20041 tgagacgtcc atcatgcgtc tgccggacga ggccattgcc tattgcccca acacggactg
    20101 cccggcgcgc ctgttggaga gcgtcataca cttcgcctcc aaaggggcaa tggacctgga
    20161 gacaatcggc gagaaacaat gcgagcaact ggtcaaagcc ggctatatta aaacggtggc
    20221 cgacttctac gatctgacgc gagagcagct gcttaagcta gagggggtca aggagaagag
    20281 cgccgacaat atgctcaagg ccatcgaggc gagtaagaag cgtcctttct ggcgtctgct
    20341 cttcggcctc aacatccgct atatcggcga aaagacagct cagctgattg ccgagcactt
    20401 cggttcaatg gatcgcctgc tgatggccag cgaagaagag atcatggcca ttcccggtgt
    20461 ggggccgaag agcgctcata gtctctatac ctggctccag caggagaaga atcgccagct
    20521 tatcgagcgc ctccgtcagg ctggcgtcaa catggccagc gaagaacggc ctggtggacc
    20581 gctggccggc cagtccttct tgttcaccgg caaactgacc cacctgacac gctcacaggc
    20641 cgaggaggca gtacgccagc tcggcggcac cgtcgccagc aacgtcacac gctcgttgac
    20701 tcacctcatt gtgggcgaag accctggctc caagctagag aaggcccagc gcgccggggt
    20761 gcagattcac gacgaacagt ggttccttga cctgctgcgc cagcacggca tcaatattag
    20821 ctcatcaggc acctgaaacc cgagcaaggg accagactgg cgagagagcc agtagactgg
    20881 tcccaccggg ccagagagag aggagggaac agcccggagc acagatgctc tcgagagtcg
    20941 cccctccttt ctaccccgca gcccagcgga cccaccccac cgtcaggggt ccgctgcggt
    21001 tcggactcag acgtagcgca tctcgagata gcgagcacgc agttgatgga cagcctcacg
    21061 cacctgcttg ccgcggatag catcgtggat cagctgagcg agttccccca ttgcgcgctc
    21121 atccatgc
//
