LOCUS       NZ_MCIF01000002        30663 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Thermogemmatispora tikiterensis strain T81 A4R35_scaffold00002,
            whole genome shotgun sequence.
ACCESSION   NZ_MCIF01000002
VERSION     NZ_MCIF01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Thermogemmatispora tikiterensis
  ORGANISM  Thermogemmatispora tikiterensis
            Bacteria; Bacillati; Chloroflexota; Ktedonobacteria;
            Thermogemmatisporales; Thermogemmatisporaceae; Thermogemmatispora.
REFERENCE   1  (bases 1 to 5961682)
  AUTHORS   Tomazini,A., Lal,S., Stott,M., Henrissat,B., Polikarpov,I.,
            Sparling,R. and Levin,D.B.
  TITLE     Analysis of Carbohydrate Active Enzymes in Thermogemmatispora T81
            Reveals Carbohydrate Degradation Ability
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5961682)
  AUTHORS   Tomazini,A., Lal,S., Stott,M., Henrissat,B., Polikarpov,I.,
            Sparling,R. and Levin,D.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2016) Faculty of Agricultural and Food Sciences,
            University of Manitoba, 66 Chancellor's Circle, Winnipeg, Manitoba
            R3T 5V6, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-MAY-2011
            Assembly Method                   :: Newbler v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 25.9x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003268475.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 03:19:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,660
            CDSs (total)                      :: 4,607
            Genes (coding)                    :: 4,541
            CDSs (with protein)               :: 4,541
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 8 of 66
            Pseudo Genes (incomplete)         :: 56 of 66
            Pseudo Genes (internal stop)      :: 6 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            CRISPR Arrays                     :: 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:11:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 263025
            Orig. end                         :: 293688
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MCIF01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..30663
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="betalactone"
                     /region_number="2"
                     /rules="(HMGL-like and AMP-binding)"
                     /tool="antismash"
     cand_cluster    1..30663
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(HMGL-like and AMP-binding)"
                     /kind="single"
                     /product="betalactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..30663
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:20663]"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..20663
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
     gene            72..1268
                     /locus_tag="A4R35_RS01225"
                     /old_locus_tag="A4R35_01190"
     CDS             72..1268
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012656.5"
                     /locus_tag="A4R35_RS01225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01190"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_112425783.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 2e-23, bitscore:
                     81.3, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 2e-16, bitscore:
                     58.1, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 6.1e-38, bitscore:
                     128.5, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYNTYLLTEEQQKLQRWAYELAQAEFQASAERWEREGTFPWEHVA
                     TLAEQGLLGFTLPRAFGGLERSRLEAVLVLEQLARVCLTTAEIAHICMNGPSYAISRLG
                     SQALQARYLPEVVAGKCLIGIAITEDEAGSSLGEMSTRAHVQATSVVINGRKCFTTIGD
                     VGGTFEVVVRFGGEGTRGLGAVLVDADTPGFVVERVYEKIGGNGIHEAALRFEHCEVPC
                     EQVLIEGDPATSNGFKLVMRAYNALRLGIAANSLGVAQAALDQLLPHLLQRRQFGAPLA
                     AAQGLRWRVARLVLALEQARLLTYRAALLSDDYGFPPAYETALAKLAATEVALQAADEA
                     IQVLGWRGITKDYPAERRYREIRGWTIAGGTSEMLLESLARQVFRRYEQSAGHAPQAE"
     gene            complement(1396..2118)
                     /locus_tag="A4R35_RS01230"
                     /old_locus_tag="A4R35_01195"
     CDS             complement(1396..2118)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF036525.5"
                     /locus_tag="A4R35_RS01230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01195"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_112425785.1"
                     /transl_table=11
                     /translation="MTQMQRQPNAARSQQTAREHVISLAERLLHPQSSHEQRLLEIVKT
                     AAAVFRERGYEAGTLEDISQQLGMTRPALYHYVRSKQDILFLTINYLLDIALAELNELE
                     RQQDTRERLRSLIIHLITLIGRERSFFTVFFRDKAGLEEPYLSLIREKERAYVAAFRRA
                     VREAMTAGMLPNIPEGIATQAILGCCNWTYQWLDPAGPVPLEQAAELIASFLVDPHPTH
                     LAGKEDTSAAVASDGQGQ"
     gene            complement(2480..2926)
                     /locus_tag="A4R35_RS01235"
                     /old_locus_tag="A4R35_01200"
     CDS             complement(2480..2926)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024844.5"
                     /locus_tag="A4R35_RS01235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01200"
                     /product="MaoC family dehydratase"
                     /protein_id="WP_112425787.1"
                     /transl_table=11
                     /translation="MTPLTPLPVVRLKVDRLKIAWMAVAITDPNPIHLEDAVAHQAGFP
                     SVIAHGTFAIGLMGLLLARWAGQQQVRRVEARLLAPVFPGEELLCQGEVMDIEEDQVQL
                     RLQVLAGERQVAVGSATVARPAAAGPPPEREVKAPLASEPGPAL"
     gene            complement(2943..4547)
                     /locus_tag="A4R35_RS01240"
                     /old_locus_tag="A4R35_01205"
     CDS             complement(2943..4547)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1124: acetyl-CoA acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF047238.1"
                     /locus_tag="A4R35_RS01240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01205"
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="WP_112425789.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 3.2e-05, bitscore:
                     21.7, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLSSSLPHILHEDWFQARGQPNLKGSRCPSCDARYFPPRQSCPG
                     CGANGLLPYEPPRTGRVLACTVIHVAPAPWQAPYAVAAVELDDGLVVYAHLSEGRAQPG
                     TPVTLDALTVRRADGEIITYAFRPLPASAGTPAAAASARQPAPQVLPPGAQPLAAPVFL
                     AGVGMVPFGKHPDVPPEILALRAGLAAIDDAGLDQRSIQAIFVGTAFGGTAMGQKILRH
                     APWGQRPVINVENACASGSTALLEAATWVASGRCDVALAIGVDTPASQGGGLIPLPPDD
                     PLAAVGIPLPALYALTADHYLHHSGASARELASLVVTSRRHAAANPYAFFRTPVTVEEV
                     LQSRPVAEPLTLFQCCPNADGAAAAVVVSAARARQLGTKVRLRAGQLRSGRVQSRFGPQ
                     SVVALTAREVYEAAGIGPEAIAVAEVHDAYSSALPPALEKLGLAPPYEAAARLAWGDYD
                     YQGAGPVVNPSGGLLSRGHPPGATGLAQVFEVVTQLRGQAGARQVAGARVGLIQNQGGT
                     VLDLETNAVAILLLTDS"
     gene            complement(4544..4963)
                     /locus_tag="A4R35_RS01245"
                     /old_locus_tag="A4R35_01210"
     CDS             complement(4544..4963)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024844.5"
                     /locus_tag="A4R35_RS01245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01210"
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="WP_189361197.1"
                     /transl_table=11
                     /translation="MDLSTIELPDVTFTVEQVKAAEFASALDTQLATEADGAALVPIGM
                     VFFVLCRDSATIFERLGVRWDRALFGGVRLEYTRQVRAGEQLVARSRFLSYRERPSAEG
                     RLGILELETCYSASDGQLVLREVSTLIARGGLELS"
     gene            complement(4944..5495)
                     /locus_tag="A4R35_RS01250"
                     /old_locus_tag="A4R35_01215"
     CDS             complement(4944..5495)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013999.5"
                     /locus_tag="A4R35_RS01250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01215"
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="WP_112425793.1"
                     /transl_table=11
                     /translation="MNEQHERMSDVALTEETVLQALESVLDPEVPVSIRQLGLIQGVHL
                     DEDEVSVALVPTMLACPGREVIAEEIRQRLSQLAGERRVVVTWETRVIWTPRRITASGR
                     QQLQRWGVVAREPTPHGVRCPYCGSYQTRLNNAFGAAVCKAQFSCSICGSAFDVLRGAL
                     RPAPAEATSQSGDGDGSVHD"
     gene            complement(5492..6178)
                     /locus_tag="A4R35_RS01255"
                     /old_locus_tag="A4R35_01220"
     CDS             complement(5492..6178)
                     /GO_process="GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016993.5"
                     /locus_tag="A4R35_RS01255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01220"
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="WP_189361199.1"
                     /transl_table=11
                     /translation="MDHVAESELRAWADDEFIVGHILGSRIIEYTHLEECLAIGSFAQD
                     EMAHARTLYEYTGLHDRALDRYVFFRAAEEFVVTPLATYQPADFPELVVKQYLYEVLDR
                     LRLQAISERTDRQLQEILPVMRYEEHVHSEHWRRWLQVLATSPARPRLEQALQVVGRYC
                     GLLPWQPSAQVQTWYGHWRPQVEQELAGQGLTLGAAAAVDWQQPFAALIEHMQSVFRER
                     PEVRLG"
     gene            complement(6252..6593)
                     /gene="paaB"
                     /locus_tag="A4R35_RS01260"
                     /old_locus_tag="A4R35_01225"
     CDS             complement(6252..6593)
                     /codon_start=1
                     /gene="paaB"
                     /inference="COORDINATES: protein motif:HMM:NF018002.5"
                     /locus_tag="A4R35_RS01260"
                     /note="with PaaBCDE catalyzes the hydroxylation of
                     phenylacetyl-CoA; involved in phenylacetate degradation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01225"
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="WP_112425796.1"
                     /transl_table=11
                     /translation="MSNQVFEVFVILKRGDPATHVGSVVAPDPVLALHEAKETYVRRTA
                     CVSLWVVPRSAITAFAPEDEILFQAAFTRDYRQAGYFTRHERQPVGPAPEPDGQSGETL
                     AVSRGGAVS"
     gene            complement(6574..7551)
                     /locus_tag="A4R35_RS01265"
                     /old_locus_tag="A4R35_01230"
     CDS             complement(6574..7551)
                     /GO_process="GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016993.5"
                     /locus_tag="A4R35_RS01265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01230"
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="WP_112425798.1"
                     /transl_table=11
                     /translation="MATAPVAAAGVRPPAPEVAEDLEAFIAWLQAGGLVQAGDPMPESY
                     RQHALRIASFQALAELVGVLLFSDWLERVPAFIRKQMITAKVQDEVGHGHVMARVAEDL
                     GGNRLTILEDYLEGRSKLLNVFHYEFQTWPEIAVGAILQNSAAIVQFQSLVKGSYLPYV
                     RALRKILPEESFHFHQARNLIQTLLGQRDPVVRRQLEEGLALWFPRVLAYFGPPEGERF
                     WSNKMVQFRLKMASNEALRQAWLTRIVPVLEQLGLQIPDPRLRRREDGTWEYTEPDWEE
                     VRRVIAGQGPASARRREQVRSIYERNRWLTEMMGIGEQHVKPGF"
     gene            7869..9572
                     /locus_tag="A4R35_RS01270"
                     /old_locus_tag="A4R35_01235"
     CDS             7869..9572
                     /NRPS_PKS="Domain: AMP-binding (55-460). E-value: 2.6e-68.
                     Score: 222.4. Matches aSDomain:
                     nrpspksdomains_A4R35_RS01270_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.5"
                     /locus_tag="A4R35_RS01270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01235"
                     /product="acyl-CoA synthetase"
                     /protein_id="WP_112425800.1"
                     /sec_met_domain="AMP-binding (E-value: 1.3e-72, bitscore:
                     242.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQRPFANVPEQFLPPPEHRAEFLFAVPWLYYIRRAQIFNAADEL
                     LDRTVRRGLGPRTAIIDADREEIWTYHRLQAAVNRCANALRALGVRPGERVVMRLNDVP
                     AAAIVQLAVWKIGVLIVPTATAERARELSFIFSDVEPTVVVSDRDYLDDVDQARAAAPQ
                     LRAVVAWPEASGRDHESFERLLATQSEEAATYPTQPLDASGIYYTGGTTGQPKGCLHTH
                     AAEVALADLNNLSRAVTADDVLFTHAPIGHAFGNGEKINFPLRAGASAIYAVRPSGERL
                     WQLAARYGATVMAGAATIYRIMLQVCPTPRERYPQLRLRQAISSGEILDQATYERWQQA
                     LGFPVRNTVGMTPIRHLFIDSNLLGEKVAPQLSVGRPLPGYEARLVDPVSGETLLDPEQ
                     PGRLAIRGPSGITYWVHRHSGILERARQDVRGGWSLLDDAYVWDQEGWLWFHARLDDMI
                     VTGGRQVAPREVEDVLTRHPSVAEAAVIGLPDPIRGQAVTAVVVLRPDQVPSEDLAHAL
                     QDFCKAQMALYKYPRRIEFVPELPKDNVGKLQRRILRERLQLLAGKEHLP"
     aSDomain        8034..9248
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R35_RS01270_AMP-binding.1"
                     /evalue="2.60E-68"
                     /label="A4R35_RS01270_AMP-binding.1"
                     /locus_tag="A4R35_RS01270"
                     /protein_end="460"
                     /protein_start="55"
                     /score="222.4"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="PRTAIIDADREEIWTYHRLQAAVNRCANALRALGVRPGERVVMRL
                     NDVPAAAIVQLAVWKIGVLIVPTATAERARELSFIFSDVEPTVVVSDRDYLDDVDQARA
                     AAPQLRAVVAWPEASGRDHESFERLLATQSEEAATYPTQPLDASGIYYTGGTTGQPKGC
                     LHTHAAEVALADLNNLSRAVTADDVLFTHAPIGHAFGNGEKINFPLRAGASAIYAVRPS
                     GERLWQLAARYGATVMAGAATIYRIMLQVCPTPRERYPQLRLRQAISSGEILDQATYER
                     WQQALGFPVRNTVGMTPIRHLFIDSNLLGEKVAPQLSVGRPLPGYEARLVDPVSGETLL
                     DPEQPGRLAIRGPSGITYWVHRHSGILERARQDVRGGWSLLDDAYVWDQEGWLWFHARL
                     DDMIVT"
     CDS_motif       8481..8534
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS01270_0001"
                     /evalue="3.40E-05"
                     /label="NRPS-A_a3"
                     /locus_tag="A4R35_RS01270"
                     /protein_end="222"
                     /protein_start="204"
                     /score="16.6"
                     /tool="antismash"
                     /translation="IYYTGGTTGQPKGCLHTH"
     gene            9569..9997
                     /locus_tag="A4R35_RS01275"
                     /old_locus_tag="A4R35_01240"
     CDS             9569..9997
                     /EC_number="3.1.2.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 4HBT_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024675.5"
                     /locus_tag="A4R35_RS01275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01240"
                     /product="thioesterase family protein"
                     /protein_id="WP_112425802.1"
                     /sec_met_domain="4HBT_2 (E-value: 6.1e-10, bitscore: 37.7,
                     seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPDSIWQPPPRALSLQVLVSMADVDSYQIHYHSYYRWIELAHHQ
                     LLRRAGMTVRELLAQGAATPAVHSECDYFIPVQVDETLTLLAWIDRPGKTSYAARCLFM
                     NAAEQVVAQGQVIHVYIEHGRPVPLPAWLLRLASDKEA"
     gene            10001..11662
                     /locus_tag="A4R35_RS01280"
                     /old_locus_tag="A4R35_01245"
     CDS             10001..11662
                     /NRPS_PKS="Domain: AMP-binding (49-452). E-value: 5.9e-63.
                     Score: 204.8. Matches aSDomain:
                     nrpspksdomains_A4R35_RS01280_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012711.5"
                     /locus_tag="A4R35_RS01280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01245"
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="WP_112425804.1"
                     /sec_met_domain="AMP-binding (E-value: 1e-69, bitscore:
                     233.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEHYPARATVPQDWLVPPELQPDFLWEQDEQTRSERNVGREAID
                     LWCNRGHGERPAVIWMTTGEVVTFQELKEKSDHLAGALIELGVEVGDRLALRYSSQPAA
                     IIAAVAAWKAGAAVVPIPAQARCSEIEFYLNDSAARWAIVLNEGDLYSELAAALPQTAV
                     RRVVIGRKAPHQDGESLETLMNEGRADLAARVRTQADDLATVWHTGGTTGRPKATYHTH
                     RRWLAAGRRLARTWGIQPGQVWLYGIPISNVAGLLGRFICTLQPGATLVEPENFSGVSV
                     LQAIVAQQVTHLLAIPVTLSQLAQEVAGDRHALASVQQVYAPFLTTGAGDLYARWQELG
                     HYLGNPLGSSIMCQWFIGPRADAILPPFALGKPVEGYEIRIVPIEGTSAEPLPAGEVGR
                     VAARGPTGLTYWNRPELQRAEVNAGWTITDDLGRMDENGIFWFMGRLNNLVVTAGYKVV
                     PGEVEEVLQQHPQVQEVAVVGLPDPLREQVVAAFVVLKDSKGDEALAQQLQQWCKERLA
                     PYKYPRKIFFRDHLPRDPVGKVQIQTLIQQAADQPV"
     aSDomain        10148..11356
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R35_RS01280_AMP-binding.1"
                     /evalue="5.90E-63"
                     /label="A4R35_RS01280_AMP-binding.1"
                     /locus_tag="A4R35_RS01280"
                     /protein_end="452"
                     /protein_start="49"
                     /score="204.8"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="RGHGERPAVIWMTTGEVVTFQELKEKSDHLAGALIELGVEVGDRL
                     ALRYSSQPAAIIAAVAAWKAGAAVVPIPAQARCSEIEFYLNDSAARWAIVLNEGDLYSE
                     LAAALPQTAVRRVVIGRKAPHQDGESLETLMNEGRADLAARVRTQADDLATVWHTGGTT
                     GRPKATYHTHRRWLAAGRRLARTWGIQPGQVWLYGIPISNVAGLLGRFICTLQPGATLV
                     EPENFSGVSVLQAIVAQQVTHLLAIPVTLSQLAQEVAGDRHALASVQQVYAPFLTTGAG
                     DLYARWQELGHYLGNPLGSSIMCQWFIGPRADAILPPFALGKPVEGYEIRIVPIEGTSA
                     EPLPAGEVGRVAARGPTGLTYWNRPELQRAEVNAGWTITDDLGRMDENGIFWFMGRLNN
                     LVVT"
     CDS_motif       10328..10363
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS01280_0001"
                     /evalue="4.50E-03"
                     /label="NRPS-A_a2"
                     /locus_tag="A4R35_RS01280"
                     /protein_end="121"
                     /protein_start="109"
                     /score="10.7"
                     /tool="antismash"
                     /translation="AAWKAGAAVVPI"
     gene            11685..13142
                     /locus_tag="A4R35_RS01285"
                     /old_locus_tag="A4R35_01250"
     CDS             11685..13142
                     /EC_number="1.2.1.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012398.5"
                     /locus_tag="A4R35_RS01285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01250"
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="WP_112425806.1"
                     /sec_met_domain="Aldedh (E-value: 1.6e-152, bitscore:
                     506.4, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGGTSVIGIDAASLVKLWIGGKACDSQSQETYPLINPFTGQVVAR
                     IARGGRADALSAIEAAARAFPLWSHLAGNERANRLYKAYHLLRRYRAELETLLIEDLGK
                     TAREAAKEVRSTGAFLRYCAEEARRIFGDFVPSPDPKKRVVVVPQPVGIVTAITASNAP
                     GILFGRKVAPALAAGCTLIVKPAEEAVRVTLLLAQLFEEAGLPSGAVNVVMGDAPAIVE
                     TLLGDPRVAMVTFTGSVQVGREVMRLAAAGPKRVVLELGGVAPFIVFADAELQAAVEGL
                     VAAKFRHAGQICASPQRIFVEERVAEPFKALLVERLARVKVGDPREEATDYGPLQHQRN
                     LQKIQRLLEDALARGARVVTGGHSPGGLLFAPTLLEGVTDEMAIAREEAFGPLIALATF
                     RDEAEVIERSNNTAYGLGAYVYTRDLARAWRLAEALEVGIVGVNDPFPATIEGPFGGVK
                     QSGSGFEGGRYGIEEFLLKKQVSFSLA"
     gene            complement(13359..14183)
                     /locus_tag="A4R35_RS01290"
                     /old_locus_tag="A4R35_01255"
     CDS             complement(13359..14183)
                     /NRPS_PKS="Domain: Abhydrolase_1 (27-258). E-value:
                     4.2e-33. Score: 106.9. Matches aSDomain:
                     nrpspksdomains_A4R35_RS01290_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516071.1"
                     /locus_tag="A4R35_RS01290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01255"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_112425808.1"
                     /sec_met_domain="PF00561 (E-value: 1.3e-23, bitscore: 82.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.1e-21, bitscore:
                     76.6, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQKTEEIGQNIVAGGVATNYHEAGQGFPVILIHGSGPGVSAWANW
                     SRVIGYLAERLHVFAYDQVGFGYTSLPADGVFGLERWVAHLFAFMDAVQVQRAHLVGNS
                     MGAAVALAAAVLHPERVERLVLMGPTGVRFPLTAGLDAVWGYTPDLEQMRQLLRLFVYH
                     QELVNDDLVALRYRASIRPGIQEAFASMFPPPRQQGIDALAAYEDRLAEIQAPTLLIHG
                     RDDRIIPLSTSLKLLHAINNVRLHVFGQCGHWTQIEYTHEFNRLVRDFLTEI"
     aSDomain        complement(13410..14102)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R35_RS01290_Abhydrolase_1.1"
                     /evalue="4.20E-33"
                     /label="A4R35_RS01290_Abhydrolase_1.1"
                     /locus_tag="A4R35_RS01290"
                     /protein_end="258"
                     /protein_start="27"
                     /score="106.9"
                     /tool="antismash"
                     /translation="PVILIHGSGPGVSAWANWSRVIGYLAERLHVFAYDQVGFGYTSLP
                     ADGVFGLERWVAHLFAFMDAVQVQRAHLVGNSMGAAVALAAAVLHPERVERLVLMGPTG
                     VRFPLTAGLDAVWGYTPDLEQMRQLLRLFVYHQELVNDDLVALRYRASIRPGIQEAFAS
                     MFPPPRQQGIDALAAYEDRLAEIQAPTLLIHGRDDRIIPLSTSLKLLHAINNVRLHVFG
                     QCGHWTQIE"
     CDS_motif       complement(13839..13889)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R35_RS01290_0001"
                     /evalue="1.20E-03"
                     /label="NRPS-te1"
                     /locus_tag="A4R35_RS01290"
                     /protein_end="115"
                     /protein_start="98"
                     /score="11.4"
                     /tool="antismash"
                     /translation="HLVGNSMGAAVALAAAV"
     assembly_gap    14661..15542
                     /estimated_length=882
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     misc_feature    15581..15594
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.12"
                     /tool="antismash"
     gene            complement(15765..16559)
                     /locus_tag="A4R35_RS01295"
                     /old_locus_tag="A4R35_01260"
     CDS             complement(15765..16559)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005203090.1"
                     /locus_tag="A4R35_RS01295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01260"
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="WP_223258131.1"
                     /transl_table=11
                     /translation="MSSGNLSPSTAEELALRLDQAEQTRTPIAPLSESYPGLTVADAYT
                     IQQHWLQKKFLRGARLVGHKVGLTSLAMQRQMGVDQPDYGFLLDSMVHSSGALLPLQQL
                     IQPRVEPEIAFWLQADLSGPHVTPAEVLAATQSVSAALEIVDSRISDWRIKLTDTIADN
                     ASSARVVVGRPIALHDLDLETEQVELRRNGEVVGTGNGAAVLGHPAAAIAWLVRKLAEF
                     NTGLRAGELVLPGAMCAAVPAQPGDRFVARFSHLGEVKVSFG"
     gene            complement(16609..18066)
                     /locus_tag="A4R35_RS01300"
                     /old_locus_tag="A4R35_01265"
     CDS             complement(16609..18066)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957297.1"
                     /locus_tag="A4R35_RS01300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01265"
                     /product="aldehyde dehydrogenase"
                     /protein_id="WP_112425811.1"
                     /sec_met_domain="Aldedh (E-value: 3.8e-168, bitscore:
                     557.9, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHPYWFDVQHYINGQFVEGERHFEILFPATNEVIGRAPEGREAEV
                     DAAVRAANTAFAYWSQMPPSQRRRYLRAFAEGIRAHEEELALTESLDVGRPIRENKASY
                     VARVAANIDFFADFAVMHGSEAYPMENGYINYVLRQPIGVAALITPWNVPMLLETWKIG
                     PALAFGNTVVLKPAELTPIGAWKLAQIAHEAGLPPGVFNVVHGFGPDSAGALLTQHPDV
                     SLISFTGETVTGKAIMSAASRTLKRISFELGGKGATLVFADSDLDLAVETSVRAAFFNQ
                     GEFCLAGPRLLVQRPLYEPFLERFLARASTLQPGNPLDPETTLGALISREHVQRVRAYI
                     DQAVAEGATLLLGGGPPHLPSPYHEGNFLSPTVIAHVQPHDRICQEEVFGPVVTVAPFD
                     SEEEALAVANGVNYGLSSVVLTRDVGRAIRVAEALKVGTVWINDFFVRDLRVPFGGMKQ
                     SGIGREGGQHSLEFYTETKTVCLSNRS"
     misc_feature    18038..18056
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 23.03"
                     /tool="antismash"
     gene            complement(18086..18823)
                     /locus_tag="A4R35_RS01305"
                     /old_locus_tag="A4R35_01270"
     CDS             complement(18086..18823)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120911.1"
                     /locus_tag="A4R35_RS01305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01270"
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="WP_223258132.1"
                     /transl_table=11
                     /translation="MMDGPINPEEAALLEEVRSARAAKCTVAGNRFHLDASLAYRIQEH
                     LGAHRPVRGYKLGLVSPAKQAQMGITTPIYGRIYDDMLLEQPVVSLSRFIQPRFEPEIV
                     ILLGEDLSPTAAIHQAQQAIAMAYLGIDLLDSIWAGYRFTLAEVIADNASGGGFLRGKK
                     PLAWPAVGTLRLFIDEHAVAEGSLEALGPVEDHLLWLAHEVGGLKAGQLIYLGSPISAQ
                     PARPGSVRVEGPQGSWLAITFLS"
     gene            complement(18820..19158)
                     /locus_tag="A4R35_RS25170"
     CDS             complement(18820..19158)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025903.5"
                     /locus_tag="A4R35_RS25170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="WP_399356013.1"
                     /transl_table=11
                     /translation="MRASPCCSIAATSAALKCRSMIPGSGSSEATGTGQKREFINNRRG
                     YMLVLKVTMLEGRSQSQKEDLIQLLSQTASRELEYPLEEIRVVIYEVSRSQWGVAGRSL
                     ACQDRRGQ"
     gene            complement(19079..19612)
                     /locus_tag="A4R35_RS01315"
                     /old_locus_tag="A4R35_01280"
     CDS             complement(19079..19612)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1160: flavin reductase domain protein FMN-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209587.1"
                     /locus_tag="A4R35_RS01315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01280"
                     /product="flavin reductase family protein"
                     /protein_id="WP_112425817.1"
                     /transl_table=11
                     /translation="MELQQQSVEAAPPQSVVDPRTFRRVLGHFATGVTVITTAYEGQVH
                     GMTANAFMSVSLDPPLVLVSIDNRTRMREWLERSRRYAVNILSSEQEAVAQHFAGRPQK
                     NLALSFLWKAELPLLEKALAHLLCQVKEVHPAGDHTLYIGLVEALEAHEGLPLLFYSGN
                     FRCLEVQIHDPWIW"
     gene            complement(19638..20663)
                     /gene="dmpG"
                     /locus_tag="A4R35_RS01320"
                     /old_locus_tag="A4R35_01285"
     CDS             complement(19638..20663)
                     /EC_number="4.1.3.39"
                     /GO_function="GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene="dmpG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271: 2-isopropylmalate synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957293.1"
                     /locus_tag="A4R35_RS01320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01285"
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="WP_112425819.1"
                     /sec_met_domain="HMGL-like (E-value: 8.1e-60, bitscore:
                     200.7, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTLRLTDTTLRDGSHAMRHQFTRQQVRSIAQALDRAGVPVIEVT
                     HGDGLGGASLQYGFSHTPELELIAEARSVCERARIAALLLPGIGTRKELKAAAERGVQV
                     IRIATQCTEADISEQHFGLTKELGLEAVGFLMMAHMRPPEVLAEQARLMESYGADCVYI
                     VDSAGALLPDGAYARVKALKEALTIQVGFHAHNNLGLAIGNTLAALEAGVDQIDGSLRG
                     LGAGAGNAATEVIAAVLDKMGQNPGLNVFALMDAAEYIVAPIAPYQPLPDRDALTIGYA
                     GVYSTFLLHARRAGELYHLDPRSILVELGRRQAVAGQEDWILDVALDLARRSQDKPVPQ
                     T"
     gene            complement(20660..21589)
                     /locus_tag="A4R35_RS01325"
                     /old_locus_tag="A4R35_01290"
     CDS             complement(20660..21589)
                     /EC_number="1.2.1.10"
                     /GO_function="GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /GO_process="GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496183.1"
                     /locus_tag="A4R35_RS01325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01290"
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="WP_112425821.1"
                     /sec_met_domain="GFO_IDH_MocA (E-value: 7e-08, bitscore:
                     31.3, seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQHSAANGRIKVAILGSGNIGTDLMYKLLKEPGSMELALVAGIDP
                     QSEGLERARSLGIPASTESIHAVLADPEIRIVFDATSAKAHMRHARLLREAGKIAIDLT
                     PAARGPYVVPPVNLGAHLDQVNVNLITCGGQATIPLVYAVSRVTPVRYAEMVSTVASAS
                     AGPGTRQNIDEFTYTTARGLEALGGAREGKAIIILNPASPPIMMRNTVYVVPEGDFDFQ
                     QVAASVEQMVSDVQRYVPGYLLKNPPVVEMRETPWGRSAVIVMLLEVRGAGDYLPTYAG
                     NLDIMTAAARRVGELFAAHLLSSKEAVA"
     misc_feature    complement(21585..21598)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.0"
                     /tool="antismash"
     gene            complement(21596..22375)
                     /locus_tag="A4R35_RS01330"
                     /old_locus_tag="A4R35_01295"
     CDS             complement(21596..22375)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018111975.1"
                     /locus_tag="A4R35_RS01330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01295"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_112433915.1"
                     /transl_table=11
                     /translation="MVQAVYKAFQILNLFSHEQPEWGVCEIARKLGFPKSTASALTASL
                     TEGGLLRRTPAGRYRLGWRIVALSQLLLETTEFRSEARRVMESLVARFGETVHLATLES
                     DQIIYIEKLQGTRAVRVAVTGIGVRLPGHCSGVGKCILAHLPWERVLAILEKQGMPALT
                     PNTITDPQILRAELEQVRRQGYAYDREEAVLELCCVAAPIRDHSGEVIAAMSLSIPAYR
                     FYANEEQYRLAIVRAAQQVSENLGYIPRIACSGKRRN"
     misc_feature    complement(22603..22616)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.4"
                     /tool="antismash"
     misc_feature    22605..22618
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.61"
                     /tool="antismash"
     misc_feature    22606..22619
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.67"
                     /tool="antismash"
     misc_feature    complement(22607..22620)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.87"
                     /tool="antismash"
     gene            22942..24522
                     /locus_tag="A4R35_RS01335"
                     /old_locus_tag="A4R35_01300"
     CDS             22942..24522
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009475043.1"
                     /locus_tag="A4R35_RS01335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01300"
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="WP_112425823.1"
                     /transl_table=11
                     /translation="MAVSQNEPAPGKEAAAVPRPLTGEEYLESLRDGREVWIYGERVKD
                     LTTHPAFRNTARMVARLYDSLHNPEKQPVLTTATDTGSGGYTHKFFVTSRSAEDLVGAR
                     DAIAEWARLSYGWLGRSPDYKASFLGTLGANAEFYEPYQENARRWYKEAQEKVLYFNHA
                     IVNPPVDRNRPPDEVSDIYMHVERETDAGIVVSGAKVVATGSALTHYNFIAHYGPLPIK
                     KKEYALIFAVPMDAPGVKLICRPSYEMTAAVMGSPFDYPLSSRLDENDAVFIFDKVLVP
                     WENIFIYGDVEKANNFFPLSGFIPRFTFHGCIRLAVKLDFIAGLFLKAVEATGAKDFRG
                     VQARVGEVLAWRNLFWAITDAMARTPLAWNNGAVLPNTDYGLAYRVFATVAYPRVKELI
                     ENDVASALIYLNSHAVDFKTPEVRPYLEKYIRGSGGYSALDRVKLMKLLWDSLGSEFGG
                     RHELYERNYAGNHENIRLEVLLSAMATGQTEQYKGFAEQCLAEYDLDGWTVPDLINPDD
                     VNRHWKQQS"
     misc_feature    complement(24631..24644)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.33"
                     /tool="antismash"
     assembly_gap    24683..25544
                     /estimated_length=862
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25545..25809
                     /locus_tag="A4R35_RS01340"
                     /old_locus_tag="A4R35_01305"
                     /pseudo=""
     CDS             <25547..25809
                     /codon_start=2
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634325.1"
                     /locus_tag="A4R35_RS01340"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01305"
                     /product="catechol 2,3-dioxygenase"
                     /pseudo=""
                     /transl_table=11
                     /translation="IQIEAGPGKHGISQAYFMYVFEPGGNRVELFGDAGYLIFDPAWKP
                     ITWHEKDLAKGIIWYGSPLPAEYFLYGTPSPEVAAALSQAAS"
     gene            25938..27440
                     /locus_tag="A4R35_RS01345"
                     /old_locus_tag="A4R35_01310"
     CDS             25938..27440
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735504.1"
                     /locus_tag="A4R35_RS01345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01310"
                     /product="aromatic/alkene/methane monooxygenase
                     hydroxylase/oxygenase subunit alpha"
                     /protein_id="WP_112425825.1"
                     /transl_table=11
                     /translation="MPKLSRPDYYDLTRDMNWRLTYVSEKEAFPPELAGDDYGIPPAEW
                     WKWDEPYKLTYREYMQNQAEKDTMSYSVKSAIARSHLFDQVDAGWKAAIAAHYGAIAIP
                     EYQASFGEARMARFGRAAAWRNMATFGTLDETRHGQIQLFFPHELLGKNPQFDWAHKAF
                     HTNEWGAIAARHLFDDMFTANHALGTAIQLTFTFETGFTNLQFLGMAADAMHVGDVEFG
                     ALISSIQTDEARHAQQGEPTLKILVRNGKKAEAQRLVDVMFWRSWRIFCLLTGLSMDYY
                     TPLEHRSMSFKEFMEEWIIKQFMDQFRDLGMDTPWYWNTFIDELDWYHHGLHLGVWFWR
                     PTVWWNPDAGVGPAERDWLEAKYPGWNDTWGKRWDVITENARKGEITKTLPQTLPIICN
                     LCQIPIITPANPGRQQELDPYVTEYGGRRYLFCSEPCKWIFDQNPGRYAGHKTVVDRFL
                     AGEIQPPTLEGALAYMGGPHLVGGRDADDFAWARDPVLAGTR"
     misc_feature    25946..25966
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 25.76"
                     /tool="antismash"
     gene            27575..27826
                     /locus_tag="A4R35_RS01350"
                     /old_locus_tag="A4R35_01315"
     CDS             27575..27826
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643052.1"
                     /locus_tag="A4R35_RS01350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01315"
                     /product="toluene-4-monooxygenase system B family protein"
                     /protein_id="WP_112425827.1"
                     /transl_table=11
                     /translation="MALIPLTAAFREDFVVQLVPVEDSDTIATVAQKVAAHAVGLRVAP
                     RQSPMQVIFQGKVLDPEKKVADVPLSPMDFVEVRYADG"
     gene            27813..28193
                     /locus_tag="A4R35_RS01355"
                     /old_locus_tag="A4R35_01320"
     CDS             27813..28193
                     /GO_function="GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735506.1"
                     /locus_tag="A4R35_RS01355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01320"
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="WP_223258135.1"
                     /transl_table=11
                     /translation="MPMDEATLTWIEVATLDELWEGEMLEVEVDGQPVLLMHLSGGEIV
                     AFQGVCPHQEYPLVEGELDLDTETLTCSGHHWQFHARSGCGINPEGCTLYRYSVRLDGS
                     TILIGYPTGSESRQRYHRCREE"
     gene            28200..28499
                     /locus_tag="A4R35_RS01360"
                     /old_locus_tag="A4R35_01325"
     CDS             28200..28499
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076041.1"
                     /locus_tag="A4R35_RS01360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01325"
                     /product="MmoB/DmpM family protein"
                     /protein_id="WP_223258136.1"
                     /transl_table=11
                     /translation="MSREHLVGPVLNDRAMFETVLEAAEIDNPGVALQTEDREGYFRIA
                     APQRLRLSRKSLEEVLGRPFRLAELEPYLSSFGGRMQIVGEEELIFYLERGAEP"
     gene            28496..29521
                     /locus_tag="A4R35_RS01365"
                     /old_locus_tag="A4R35_01330"
     CDS             28496..29521
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005568702.1"
                     /locus_tag="A4R35_RS01365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4R35_01330"
                     /product="aromatic/alkene monooxygenase hydroxylase subunit
                     beta"
                     /protein_id="WP_112425831.1"
                     /transl_table=11
                     /translation="MTSSSPSTRQQRRQRSWSLYTEQRKPSEYEIVTHRLNYHFRRAPA
                     PFELDPDMPLNRWYLRYREGSPFQVDDWEGFRDPARLTYRAYIQRQRERELYLDNLIDE
                     FERRDHFALLKPDWVCFLEHLYLPSRFAGHVLQMVSLYVSQMAPSSYITVAFHFQGADE
                     MRRVQRSAYVAKALSLAHYQELADSQKTRAIWETTPAWQPARELLEKLLIAYDWGEAFT
                     ALALVTKPVYDAFFNEQLGTLARLNGDDLLALMADDFALDQKRHQDTARALVLYALERK
                     PELRTTLNGWVAKWAPAAHRAVEGLATFFSWAPHPLEKKAVVQAVGQRLQDFLKECQLS
                     L"
     gene            29573..30160
                     /locus_tag="A4R35_RS01370"
                     /old_locus_tag="A4R35_01335"
     CDS             29573..30160
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A4R35_RS01370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A4R35_01335"
                     /product="luciferase family protein"
                     /protein_id="WP_146747077.1"
                     /transl_table=11
                     /translation="MRANCQVTPSQEDQSSPVVTDMKSSYGYLPPSLRRLPVRQGERPL
                     INPRYPCLQDNHWAPPRIREQFLRWLAKLEGVSLESSDLPPAAQALVLAEHLAKGKPEA
                     FIRGKTFAIVRADGSVHLLLEPAWGQKVLDKGWGSVHPLARYMAGAIPPQSLILYAPRD
                     QRDLAVLRRIILAAYSFACGRIGTQILPDTAW"
ORIGIN
        1 agcatggagg atcgggcagg ccactcgtcg gcagctatgc cgacgcggta gcacagcagt
       61 cggaggtagt catgtacaat acgtatctgc tcacagagga gcagcagaag ctgcagcgct
      121 gggcctacga gctggcgcaa gctgagtttc aggccagcgc cgagcgctgg gagcgtgagg
      181 ggacctttcc ctgggagcat gtggcaacgc tggcggagca gggactcctt ggctttaccc
      241 tgccacgagc cttcgggggc ctggagcgct ctcgcctgga agccgtgctg gtcctggagc
      301 aactggcgcg cgtttgtttg actacagcgg aaatcgccca tatttgcatg aatggtccat
      361 cctatgctat cagtcgttta ggcagccagg ccctgcaggc caggtacctg ccggaggtcg
      421 tcgcggggaa atgtctcatt ggcatcgcga tcaccgagga cgaggccggc tcgtcgctgg
      481 gcgagatgag cacgcgggca catgtccagg cgacgtcggt ggtgatcaat gggcgcaagt
      541 gcttcaccac catcggggac gtcggcggca cctttgaagt tgtggtgcgc tttggtggcg
      601 agggcacgcg cggtttgggg gctgtgctcg tggatgccga cacgccgggc ttcgtcgtcg
      661 agcgagtcta cgagaagatc gggggcaacg gcattcacga ggcagcgctc cgttttgaac
      721 attgcgaggt gccgtgtgaa caggtgctga tcgagggcga tcctgccaca agcaacggct
      781 ttaagctggt gatgcgcgcc tataatgccc tgcgcctggg gatcgccgcc aactcgttgg
      841 gggttgctca ggcggcgctc gatcaactgc tgccccattt attgcagcgt cgccagttcg
      901 gggcgccgct ggctgccgct caagggttgc gctggcgagt tgcgcgcctg gtcctggctc
      961 tggagcaggc tcgtctactg acgtatcgcg ccgcccttct ctccgacgac tacggtttcc
     1021 ctccagccta tgaaactgcc ctggccaagc tggcggccac agaggtagcg ctccaggcag
     1081 cagatgaggc cattcaggtt ctcggctggc gcggcattac caaagattac ccggcagagc
     1141 gccgctatcg tgagatccgc ggctggacca tcgccggcgg caccagcgaa atgctcctcg
     1201 aatcgctggc caggcaggtc ttcaggcgct acgagcagtc tgccggccac gcgccacagg
     1261 ccgagtaaac ctgggtagag tggcgaagaa gggccggggc ctcactcagc tctgctcaga
     1321 ttcatggtgc ggtgtcggtg gcagcggcgc gcggtgttcc ttcctccctg gggatggtgc
     1381 tcctctgccg ccggctcatt gtccctggcc gtctgaggcc actgccgctg aggtgtcttc
     1441 ttttccggcg aggtgcgtag ggtggggatc aacaaggaag gaggcaatca gttccgctgc
     1501 ctgctccaac ggcacgggcc cagccgggtc aagccactga taggtccagt tgcagcagcc
     1561 gagaatggcc tgggtggcta tcccttcggg gatattgggc agcatgcctg ccgtcatcgc
     1621 ctcgcgaacg gcacgacgaa aagctgccac gtaggcccgc tctttctcgc gaatcagtga
     1681 gagatatggc tcctccaggc ctgccttgtc tcggaagaaa accgtgaaga agctgcgctc
     1741 ccgtccaatc agcgtgatca aatggatgat cagcgagcgc aggcgctccc gtgtgtcctg
     1801 ctggcgttct aattcattca gctcggcgag ggcgatatcc agcagataat tgatagtcag
     1861 gaagagaata tcttgcttgc tgcgtacgta gtggtacaga gccgggcggg tcatgcccag
     1921 ttgctgagag atatcttcca gggtaccggc ttcgtagccg cgctcacgaa agaccgctgc
     1981 cgcagttttg acgatctcca gcagacgttg ctcgtgggaa ctctgaggat ggagcagtcg
     2041 ctcagccagg ctgatgacat gttctctagc cgtctgctga ctgcgggcag cattcggttg
     2101 gcgctgcatc tgtgtcattg aagattacgc actctcctat cagtccaggt atggttgggt
     2161 acagtggtag tttagacggg gcctgtctcg ctctggcact gcgcgctcgg gtcgatcgcc
     2221 cagagcaatt ccccgcagca cggctgctgg acagaattgg agcaccacca gagttgacgc
     2281 tcctgtccct tgctgagccg cctgtgtgct cgctcctggc agtatttatt atccttcttc
     2341 tccccccaat ctgacaaggg aaaaaagtgc tgccgccagc caggaggagc ctgcctgtcc
     2401 cacggagagc agtatgccag ggcgctcgct ctgacctgac tgacggcgac cctggcatac
     2461 ctggaaaagg atctctctct cacagggcag gccctggctc tgaggcaagc ggcgccttca
     2521 cctcgcgctc gggcggtggg ccggctgcgg ctggcctggc gaccgtggcc gaacctaccg
     2581 ctacctgccg ctcgccggcc agcacttgta gccgcagttg tacctgatct tcctcgatgt
     2641 ccatcacctc tccctgacag agcagctcct cgcccgggaa aacaggcgcc agcagacgcg
     2701 cctctacacg gcgcacttgc tgctgaccag cccagcgcgc caggagcagg cccatcaagc
     2761 caatagcaaa ggtgccatga gcaatgacgc tggggaatcc cgcctgatgg gcgacggcgt
     2821 cctccaggtg gatcggattg gggtcagtga tggcgaccgc catccaggca atcttgaggc
     2881 ggtctacttt gagcctgact actggcaacg gtgtcaacgg ggtcataagg cacgttcctc
     2941 tgttatgaat ccgtgagaag cagaatagcc acggcgttgg tttccagatc gaggactgtt
     3001 cccccttgat tctggatgag tcccacacgc gcgccagcta cctgcctggc accggcctga
     3061 ccacgcaact gggtgaccac ttcgaagacc tgggccaggc ctgtggcccc agggggatga
     3121 ccgcgtgaga gcagccctcc gctgggattg accaccgggc cagccccttg atagtcatag
     3181 tcgccccatg ccagacgagc cgcagcctca tacggcggcg ccagtccgag cttctccagg
     3241 gccggcggca gagccgagct ataggcgtcg tgaacctccg ccaccgcgat cgcttcgggg
     3301 ccgataccgg cggcctcata gacctctcgc gccgtcagag cgacgacact ctgcggcccg
     3361 aaacggctct gaaccctgcc gctgcgcaac tgtccggcac gcagtcgcac cttggtcccg
     3421 agctggcgcg cgcgcgcagc gctgacgacc accgccgccg cggctccgtc agcgttggga
     3481 cagcactgga agagggtgag cggctcggcc accgggcgcg attgcagcac ctcctcgacg
     3541 gtcaccggcg tacggaaaaa ggcataggga ttggccgcgg catgccggcg gcttgtgacc
     3601 accaggctcg ccagctcgcg cgcgctggcc ccagagtggt ggagatagtg atcggcggtg
     3661 agggcgtaga gggccggcag gggaatgccc accgccgcca gaggatcatc gggcggcaag
     3721 ggaatcaggc ctcctccctg cgaggccggg gtatcgacgc cgatcgccag cgccacatcg
     3781 cagcgcccgc tggccaccca ggtggcagct tcgagcaggg ccgtgctgcc agaagcgcag
     3841 gcgttttcaa cgttgatcac tgggcgctgt ccccaggggg catgacgcaa gatcttctgt
     3901 cccattgctg tcccgccgaa ggccgtgccc acgaaaatgg cctggatgga ccgctgatcg
     3961 agtccggcat catctatcgc tgccagtccg gctcgcaacg ccagaatctc cggaggcaca
     4021 tccgggtgtt tgccaaaggg gaccatgcca acgccggcaa ggaagactgg ggccgctaag
     4081 ggctgcgctc ctggcgggag aacctgggga gctggctgcc ttgcgctggc agcggcagcc
     4141 ggggttccag cagaggcggg caaggggcga aaggcatagg tgatgatctc cccatcggcg
     4201 cgcctgactg tcagagcatc gagagtcacc ggcgtgccgg gttgggcgcg cccctcgctc
     4261 aggtgcgcgt agacgacaag accatcatcc agctcgaccg ccgccactgc gtagggagcc
     4321 tgccaggggg cgggcgccac atggatgaca gtgcaggcca gtacacggcc tgtgcgcggc
     4381 ggctcatagg gcagcaaccc attggcccca cagccgggac aggattgccg cggtgggaag
     4441 tagcgtgcgt cacagctggg acagcgcgac cctttcaagt tcggctgacc acgtgcctga
     4501 aaccagtcct cgtgaagaat atgcggtaag gaagatgaga gagtcatgaa agctccagtc
     4561 ctccgcgggc aatcagggta ctgacctccc gcaacacaag ctgtccgtca gaggcagagt
     4621 agcaggtctc cagctccaga atgccaagcc gtccttccgc actggggcgc tcgcgatagc
     4681 tcagaaagcg gctgcgggcc acaagctgct cgccagcacg tacctgacga gtatattcca
     4741 gccggacccc gccgaagagc gcacggtccc agcgcacccc gaggcgctcg aagatagtgg
     4801 cgctatcgcg gcagagcacg aagaagacca tgccgatagg aaccagggcg gcgccgtcgg
     4861 cctcggtggc gagctgtgta tcaagggcgc tcgcaaactc ggcggccttg acctgctcca
     4921 ccgtgaaggt cacatcaggt agctcaatcg tggacagatc catcgccgtc tccgctctgg
     4981 ctggtagcct ccgctggtgc gggtcgcaag gcgccgcgca gcacatcaaa ggctgatcca
     5041 cagatgctac atgaaaactg agctttacag acagccgcgc caaaagcgtt gttgaggcgc
     5101 gtctggtagg agccgcagta tgggcagcgg acgccgtggg gcgttggttc gcgtgctacg
     5161 acgccccagc gctggagctg ctgacgcccg ctcgcagtga tacgccgcgg agtccagatc
     5221 acgcgcgtct cccaggtgac caccacgcga cgctcgcccg ctagctggct taagcgctgg
     5281 cggatctcct cagcgatgac ctccctgccc gggcaggcca gcatggtagg cacaagggcg
     5341 acactgacct catcctcatc cagatgcacc ccctggatca ggcccaactg tcgaatgctg
     5401 accggtacct cgggatcaag aacgctctcc agtgcctgga gaaccgtttc ttcagtgaga
     5461 gcgacatcgc tcatacgctc gtgctgttcg ttcatcctag cctcacctct ggccgctcgc
     5521 gaaagaccga ctgcatatgt tcaatcaagg cggcaaaggg ctgctgccag tcgacagcag
     5581 cggcggcccc aagggtcagc ccttgcccgg ccagctcctg ctcgacctgc gggcgccagt
     5641 gcccatacca ggtctgaacc tgagctgacg gctgccaggg gagcaggcca caataacgcc
     5701 cgacgacctg cagggcctgc tccaggcgag ggcgggccgg cgaggtggcc aggacctgca
     5761 accagcgccg ccagtgctcg ctgtggacgt gctcttcata gcgcatgacc ggcagaatct
     5821 cctgcagctg ccggtccgtg cgctcgctga tggcctgcag gcgcaggcga tctaatacct
     5881 cgtagagata ctgcttgacg acaagctccg ggaagtccgc tggctggtag gtggccaggg
     5941 gcgtgacgac aaactcttcc gccgctctga agaagacata gcgatcgagt gcgcgatcgt
     6001 gtaagccggt atattcgtag agcgttcgcg catgagccat ctcatcctgg gcaaacgagc
     6061 cgatggccag gcattcctcc agatgggtat attctatgat gcggctcccc aagatatggc
     6121 caacgatgaa ttcgtcgtcg gcccaggcgc gtagctcaga ctcagctacg tggtccatct
     6181 cgtggttccc ttccttcctt gcttcctttc ttccttcccc cccttccctg gatgctgcct
     6241 ctggcgggcg ctcatgagac agccccccca cgtgagaccg ccaatgtctc tcctgactgg
     6301 ccgtctggct ccggcgctgg ccccactggc tgccgctcgt gccgcgtaaa gtagcccgcc
     6361 tggcggtaat cgcgggtgaa tgcagcctga aagaggatct catcctccgg cgcaaaggcg
     6421 gtgatggcgg agcgcggcac gacccagagc gagacacatg cggtgcgccg cacataggtc
     6481 tctttggcct cgtggagcgc cagcacggga tcaggcgcaa ccacgctgcc gacgtgcgtc
     6541 gccgggtcgc cgcgcttgag gatcacgaag acctcaaaaa cctggtttga catgttgctc
     6601 acctatgccc atcatctcgg ttaaccagcg attgcgctca tagatgctgc gtacctgctc
     6661 tcgacggcgc gctgaggccg gcccctgccc ggcgatgacc cgccgcactt cctcccaatc
     6721 aggttcggtg tattcccagg taccatcttc tcggcgccgc agccgcgggt cgggaatctg
     6781 caaaccaagc tgttcgagca cggggacgat cctggtgagc caggcctggc gcagcgcttc
     6841 gttactggcc atcttgagac ggaattgcac catcttgttg ctccagaagc gctctccctc
     6901 gggcggaccg aagtaggcca ggacgcgcgg gaaccagagc gctaacccct cttcaagctg
     6961 acggcgcaca acaggatcgc gctgcccaag cagggtctgg atcagattgc gcgcctgatg
     7021 aaagtggaag gactcttcgg gcaagatctt gcgcaaggcc cgcacgtagg ggagatagct
     7081 gcctttgacc agactctgaa actggacgat ggccgccgag ttctgcaaga tggcacctac
     7141 ggcgatctcg ggccaggtct gaaattcata gtggaagacg ttgagcagct tgctccgccc
     7201 ctctagataa tcttccagga tggttaagcg gtttcctccc aggtcctcgg ccacccgcgc
     7261 catgacatgc ccatgcccta cttcgtcctg cactttggcg gtaatcatct gcttgcggat
     7321 aaaggcaggg actcgctcaa gccagtcgga gaagagcagt acacccacaa gctccgctaa
     7381 cgcctgaaac gaggcaatgc gcagggcatg ctggcgatag ctctccggca tcggatcgcc
     7441 agcctgtacc agtcctcccg cctgcaacca ggcaataaag gcctccagat cctcagcgac
     7501 ctcgggcgcg ggcggtcgta cccccgctgc ggcaacaggt gctgtggcca tgtattcctc
     7561 cttcttcccc ccttgacaag gggcagtctc cgcagtagac ttacttacta gtaagtaaac
     7621 tatagcacgc ggggaagagg ggtgtcaagg agttcggcgt tacttactta ctagtaagta
     7681 aaatatccag agaaggaagg gcgaccctct ctagcgagac agagagcgtg cggtctgtga
     7741 gcgcagtcta cagtgaagtc aggcagtcca ctgtccgtag cgcctgtctg gccagccgcc
     7801 tctcaggcgt agccaggatc ggacgagcag cgtggccggg cgatcctatt aaaaaggagg
     7861 aggatctcat ggagcagcga ccttttgcca acgtgccaga gcagtttcta ccgccgccgg
     7921 aacaccgggc cgagtttctt ttcgctgtgc cctggctcta ttacatccgg cgagcccaga
     7981 tcttcaacgc tgcggacgag ctgctagatc gcacggtgcg gcgaggtttg gggccacgga
     8041 cggccatcat cgatgccgat cgagaggaga tctggaccta ccatcggctg caggcagcgg
     8101 tcaatcgctg cgccaatgcc ctgcgggcgc tgggtgtacg acctggcgag cgggtcgtga
     8161 tgcggctcaa cgacgtgcct gctgcggcga tcgtacagct agcggtctgg aagatcgggg
     8221 tcttaatcgt ccccacagcc acggctgagc gagcgcgaga actctccttt attttctccg
     8281 atgtcgagcc gacagtcgtg gtctctgatc gtgactacct ggacgacgtt gatcaggccc
     8341 gcgcggcggc gccccagttg cgggcagtgg tggcctggcc cgaagcctcg gggagagacc
     8401 acgagagctt tgagcgactg ctggcaaccc agtcagagga ggcggcgacc taccccaccc
     8461 agccgttgga tgccagtggc atctattaca ccggcggcac caccggtcag ccaaagggct
     8521 gcctccatac ccatgctgct gaggtggcgc tggcggacct caataacctg agtcgcgccg
     8581 tcactgctga tgatgtgctc ttcacgcatg cccctatcgg tcatgccttt ggcaacggcg
     8641 aaaagatcaa ctttcccctg cgtgccgggg ccagcgccat ctatgccgtg cgcccttccg
     8701 gtgagcggct ctggcagctc gcagcccggt atggcgcgac tgttatggcc ggagcagcga
     8761 ccatttaccg catcatgctc caggtctgtc cgacgccgcg cgagcgctat ccgcagttgc
     8821 gactgcgcca ggccatcagc tccggtgaga ttctggatca ggccacgtac gagcgctggc
     8881 agcaggctct aggcttcccc gtcagaaaca ccgttgggat gacgccgatt cgtcacctgt
     8941 ttatcgactc caatctgctg ggggagaagg tggcgcccca gttgtcggtt ggtcgtcccc
     9001 tgccaggcta cgaggctcgc ctggttgacc cggtgagtgg cgaaacgctg ctagaccccg
     9061 agcagccggg gcgcctggcg atccgcggcc ccagcggcat cacctattgg gtgcatcggc
     9121 atagcggtat tctggagcgg gcccgtcagg acgtgcgtgg gggctggagc ctgctcgacg
     9181 acgcctacgt ctgggatcaa gagggctggc tctggttcca tgcgcggctg gacgatatga
     9241 ttgtgactgg cggcagacag gtggcgccga gggaggtgga ggacgtgctg acgcgccatc
     9301 cctccgtggc ggaagcggcg gtgattgggc tgcccgatcc gatccgcggt caggctgtga
     9361 cagcagtggt agtgctgcgc ccggaccagg tgccttccga ggatctcgcc catgctctcc
     9421 aggacttctg caaggcccaa atggcgctct acaagtatcc gcggcgcatc gaattcgtac
     9481 cggagctgcc gaaagataac gtcggcaagc tgcagcgacg aatcttgcgc gaacgcctgc
     9541 agctcctggc tggaaaggag catctcccgt gaagcccgac tctatctggc agcctccgcc
     9601 gcgggccttg agcttacagg tcctggtctc aatggccgac gtcgatagct atcagatcca
     9661 ctaccactcg tattatcgct ggatcgaact ggctcaccac cagctgctgc gtcgcgccgg
     9721 catgacggtc cgcgagctgc tggctcaagg ggccgccacg cccgcggtcc atagcgagtg
     9781 tgactacttc ataccagtcc aggtggatga gactctcacg ctgctggcct ggattgatcg
     9841 tcccggtaaa accagctatg ctgcacgatg tctgtttatg aacgcagcag aacaggtcgt
     9901 cgcccagggc caggtgatcc acgtctacat tgagcacggt cgcccggttc ctctgccggc
     9961 ctggctcttg cgtctcgcct ctgacaagga ggcttgagcc atgaaggagc attatccagc
    10021 aagggctact gtcccgcaag attggcttgt tcctcccgag ctgcagcctg actttctctg
    10081 ggagcaggat gagcagacac gctctgagcg caacgttggt cgcgaagcga tcgatctctg
    10141 gtgtaacaga ggccacggag agagacctgc tgtcatctgg atgacaacag gagaggtcgt
    10201 cacctttcag gagctgaagg agaagagcga tcaccttgca ggggctctga tcgagcttgg
    10261 cgttgaggtc ggcgacaggc tggcgctacg ctatagcagt cagccagccg ccatcattgc
    10321 cgccgtggcc gcctggaaag ccggtgctgc ggttgtgccc attcctgcgc aggcgcggtg
    10381 cagcgagatc gagttctatc tgaacgatag tgccgcgcgc tgggccatcg tgctcaacga
    10441 gggtgacctc tactctgagc tggctgcggc gctgccgcag accgcagtca ggcgcgtggt
    10501 gattggcagg aaagcccccc accaggatgg cgagagtctg gagacactta tgaacgaggg
    10561 ccgcgccgat ctggctgcac gcgtgcgcac gcaggcggat gacctggcga cagtctggca
    10621 caccggcggc acaacagggc ggcccaaagc cacctatcat acacaccggc gctggctggc
    10681 ggcgggccgc cggcttgcgc gcacctgggg catccagccg gggcaggtct ggctgtatgg
    10741 tatccccatc agcaatgtcg ccgggctgct gggcaggttt atctgcacgt tgcaaccggg
    10801 cgccactctt gtggagccag agaacttcag cggcgtcagc gtgctccagg ccatcgtcgc
    10861 tcagcaggtg acacacttgc tggccattcc cgtgacgctg agccagctgg ctcaggaggt
    10921 ggctggcgat cgacatgctc tcgccagcgt gcagcaggtc tatgctccct tcttgacaac
    10981 gggtgccggt gatctctacg cccgctggca ggagctgggc cattatctgg gcaatcccct
    11041 cggcagctct atcatgtgcc agtggtttat cggaccacgc gctgatgcca tcttaccgcc
    11101 cttcgcgctc ggtaaaccgg tcgagggcta tgagatacgc atcgtcccca tcgagggcac
    11161 gagtgctgag ccgctgcctg ccggagaggt tgggcgcgtc gccgccaggg gaccgacggg
    11221 actcacctac tggaaccgcc cggagctgca gcgagcggag gtcaatgccg gctggacgat
    11281 caccgatgat cttggccgca tggatgaaaa cggcatcttc tggttcatgg ggcgcctcaa
    11341 taacctggtt gtgacagccg gctacaaggt ggttcctggc gaggtcgagg aggtcttgca
    11401 gcagcatcca caggtccagg aggtcgcagt cgtcggtctg cccgaccccc tccgggagca
    11461 ggtggtcgcc gcttttgtgg tgctcaagga cagcaagggc gatgaggctc tcgctcagca
    11521 gttacagcag tggtgcaaag agcgtctggc tccctataag tatccgcgca agatcttctt
    11581 ccgcgaccat ctgccgcgtg atcccgttgg caaagtgcag atccagacac tgattcagca
    11641 ggcagcagat cagccagtct agacgagcag acaggcagag aaacatggga ggaacatccg
    11701 tgattggtat cgatgctgcg tctctagtga agctctggat cggcgggaag gcctgcgaca
    11761 gccagagcca ggagacctat cctctgatca acccctttac cgggcaagtg gttgcccgga
    11821 tagcccgagg agggcgggcc gatgcgctaa gcgctatcga ggcagcggcc cgcgccttcc
    11881 cgctctggtc gcacctggcc gggaatgagc gggccaacag gctctacaaa gcctatcact
    11941 tgttgcgccg ctaccgggcg gagctggaga cgctcctgat cgaggacctg gggaagactg
    12001 cccgcgaggc ggccaaagag gtccgctcta ccggggcgtt tctgcgctat tgcgccgagg
    12061 aggccaggcg catcttcggg gatttcgtgc cctcgcctga tccaaagaag cgtgtcgtag
    12121 tcgttcctca gccggtgggc atcgtgacag ccatcacagc ctcaaacgcg ccgggcatcc
    12181 tcttcgggcg taaggtggcc ccggcgctgg ccgctggctg cacgctcatt gtgaagccag
    12241 cagaagaggc ggtgcgggta acgcttctgc tcgctcaact gtttgaggag gcagggctgc
    12301 cctccggggc cgtcaacgtc gttatgggag atgccccggc tatcgttgaa accctccttg
    12361 gtgatcctcg cgtggcgatg gtgaccttca cgggctcggt ccaggtcgga cgtgaggtga
    12421 tgcgcctggc cgctgcgggg ccgaagcgcg tcgtgctgga actggggggc gttgctccct
    12481 ttattgtctt cgccgatgcc gagctgcagg ccgcagtcga gggactggtc gccgctaaat
    12541 tccgccatgc cgggcagatc tgcgccagtc ctcaacggat ctttgtcgag gagcgggtgg
    12601 ccgaaccttt caaggcgctc ctggttgagc gactcgcacg tgtcaaggtg ggcgatccgc
    12661 gggaggaggc taccgactat ggccctctcc agcatcagcg caacctgcag aagattcagc
    12721 gcctgctgga ggacgcgctg gcccgtgggg cccgtgttgt cactggaggc cacagcccag
    12781 gtggcttgct ctttgctcct acgttgctcg aaggagtcac cgacgagatg gccattgccc
    12841 gcgaggaggc ctttgggccg ctgatcgccc tggccacttt ccgcgacgag gccgaggtca
    12901 ttgagcgtag caacaatacg gcctatggac tgggagccta cgtctatacc cgcgatctgg
    12961 cccgcgcctg gcgcttggct gaggccctgg aagtgggcat tgtgggggtg aacgatccct
    13021 ttccggccac gattgaagga ccctttggcg gggtaaagca gtccggctcc ggatttgaag
    13081 ggggccgcta cggcattgag gaattcttgc tgaaaaagca ggtctctttc tcgctcgcct
    13141 gagccgtgaa gtgaagggcc atccctggcg accagttcgg ggaagagaca tcgctcaacg
    13201 ggtgagcgag cgacgcttcc cccgtccgcc agacccactg ctgggggcaa ctgcccgctc
    13261 tgctctcaag tgccggatca gatcgccctg gtcataccat gccacagatc agcagttttt
    13321 ctgtccgtca agcctgacca gcacgcatca cacgaatatt agatctctgt taagaaatct
    13381 cgcacgagtc ggttgaattc gtgtgtatat tcaatctgcg tccagtgacc acactgacca
    13441 aagacgtgca accggacatt attgatagcg tgcagcaatt ttaagctggt tgagagcggg
    13501 ataattcggt cgtcacgtcc atgaatcaga agagtcgggg cctggatctc cgccagccgg
    13561 tcctcgtagg ccgccagcgc atcgataccc tgctggcgtg gcgggggaaa catgctggca
    13621 aaggcctcct ggatacccgg gcggatactt gctcgatagc gcagggccac caggtcatcg
    13681 ttcaccagct cttgatggta gacgaagagc cggaggagct gtcgcatctg ctccagatcg
    13741 ggagtgtagc cccaaacggc atccaggccg gcggtcaagg ggaaacgcac tcctgtcggc
    13801 cccatcagta ccaggcgctc gactcgttct ggatggagca ctgccgctgc cagcgctact
    13861 gctgccccca tcgagttccc caccagatga gcgcgctgta cctgcacagc atccataaag
    13921 gcaaacagat gagccaccca gcgctccaga ccaaaaacgc catcggcagg caaggaggta
    13981 tagccgaagc cgacctggtc ataggcgaac acatgcagac gctcggccag atacccaatg
    14041 acccggctcc aattggccca ggcggagacc cccggcccag agccatgaat caatatcaca
    14101 ggaaagccct ggcccgcctc atgatagttg gttgccaccc ctccggcaac aatgttctgc
    14161 ccgatttctt ctgtcttctg catagagagt actctccttc cttagtctcc caaactctgg
    14221 ttgggaatcg tatcgtccag tggtgcactg agtagatcgc tggtgccctg gagagggacc
    14281 tcgcgggcac cagccatgct ctctctggag aggagaccag ggtggattgg tgttcgttcg
    14341 ctaccactgc caccgtcctt cccggcctct ctccataaag gtatgccgcc ggggacccct
    14401 ggattcatcc aggggaggaa ggtggctccc tcctggctct ttgccggggg ttgagatagc
    14461 agcctctctc ctggtatcct tgcgctatga ggcttcaaga cactgacctg tgagtcagca
    14521 acggcttcgc ctcacctggc agggggaggg tcaacccagt cacaaggctt tgctgtccta
    14581 cggggcagtg ggctgttgtc aaccagccct gaagcccggg ccgctgcggc tgcgggcaag
    14641 cccctggctt gagccggggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nngggagtca ggagctcgac atgagccagc tgggctacct caaacatggg ttcctgtccg
    15601 ttttctgcca ttgttgcctc cttgatcctt gctaaatttc agctgccgct atggcaggcc
    15661 ggatctgacg gcctctggag accgtcgtcc agcgcccggg gcaattagtc cgacatagac
    15721 ccctggcctt cctctcaggc tgtggcccat cgaaggcgag agcactatcc aaaggagact
    15781 ttcacctccc ccagatgcga aaaacgcgcc acgaaccgat cgcctggctg cgcagggaca
    15841 gcggcgcaca tcgctcctgg taacaccagc tccccggcac gcagtcctgt attgaactca
    15901 gcgagcttcc tgactaacca ggcaatggcg gctgccgggt gacccaggac tgccgcgcca
    15961 ttgcccgttc cgacaacctc gccgttgcgc cgaagctcca cctgctcagt ctccaggtcc
    16021 agatcgtgaa gcgcgatcgg gcgcccgacc acaacccgtg ctgaggaggc attgtctgca
    16081 atggtatcgg tgagcttaat gcgccaatcg ctgatgcggc tatcgacgat ctccagcgca
    16141 gcgctcacgc tctgcgttgc cgccagcacc tcagcgggag ttacatgggg gccggagagg
    16201 tcagcctgaa gccagaaggc aatttccggc tcaacgcggg gctggatcag ctgctgcaaa
    16261 gggagaaggg cgcccgagga gtgcaccatc gagtcgagca ggaaaccata atcaggctgg
    16321 tcgaccccca tctggcgctg catggccaga ctggtgagtc ccaccttgtg accgacgagg
    16381 cgagcacctc gtagaaactt cttctgaagc cagtgttgct ggatggtata ggcatcggcc
    16441 acagtgaggc ccggatagga ttcgctcagg ggcgcaatgg gagtgcgtgt ctgctccgcc
    16501 tgatcgagcc gtagagctag ctcttcggca gtagaggggg aaagattgcc actggacacg
    16561 gttctcctcc tgctcatact tggacatggt cactgtccaa ggcttatgct atgatcggtt
    16621 actcaagcaa acggttttgg tctcagtgta gaactccagg ctatgctggc caccttcccg
    16681 tccaatgccg ctctgcttca tccctccaaa gggcacgcgc aaatctcgga caaagaaatc
    16741 gttaatccag actgtcccca ccttcagagc ctctgctact ctgatagcgc gcccgacatc
    16801 acgggtcagc accacagacg aaagtccata gttgacccca ttggccaccg ccagagcttc
    16861 ctcctcacta tcgaagggtg cgaccgtcac gaccggtccg aagacctctt cctggcagat
    16921 acggtcatga ggctggacat gagcgatcac cgtaggagag agaaaattcc cttcatggta
    16981 tggagaggga agatgaggcg gcccgccccc cagcaagagc gtcgcccctt cagccacggc
    17041 ctgatcgatg taggcccgca cacgctgcac atgctcacga gagattaagg ctcccagcgt
    17101 cgtctccggg tccagaggat tacccggctg cagggtgctg gctctggcca gaaagcgctc
    17161 aagaaaaggc tcatagagcg ggcgctgtac caacaaacgt ggaccagcca ggcagaactc
    17221 gccttgattg aagaaagcgg ctcgaacact ggtctccacc gccagatcca gatcgctgtc
    17281 ggcgaatacc agcgtggccc cctttccccc aagctcaaaa gagatgcgct tcagcgtacg
    17341 agaagcagcg ctcataatgg ctttaccggt gacagtctca ccggtgaaag aaatgagact
    17401 cacgtcagga tgctgggtca gcaaagcccc tgccgagtcg gggccgaagc catgcaccac
    17461 attgaagacg cccggtggga ggccggcctc atgagcaatc tgggccagct tccaggcccc
    17521 gatgggggtc agctccgccg gtttcaagac cacggtattc ccgaaggcca gggccgggcc
    17581 aatcttccag gtctccagga gcattggaac attccagggt gtgatcaagg ccgcgacacc
    17641 tatcggctgg cgaagcacat agttaatata gccattctcc atcgggtagg cctcgctgcc
    17701 atgcattacc gcgaaatccg cgaaaaagtc gatgttggcc gcgacgcgcg caacgtagct
    17761 cgccttattc tcgcggatcg ggcgcccgac atccaggctt tcagtgagcg ccagctcctc
    17821 ctcatgggcc cgaataccct cggcgaaagc ccgtaagtac cgccgtcgtt gtgagggggg
    17881 catctggctc cagtacgcga aagcggtgtt ggcggctcgt actgcggcat caacctcggc
    17941 ctctcggcct tctggtgccc gccctatgac ctcattcgtg gccggaaaca agatctcaaa
    18001 gtgtctctct ccctccacga actggccatt aatatagtgc tgtacatcga accagtaggg
    18061 gtgcatgcca gcgaatcctc cgtttctagc tcaaaaacgt aatggcaagc cagctccctt
    18121 gaggtccctc tactctgaca ctgccagggc gagccggctg ggcgctgatt ggagagccga
    18181 ggtagatcag ctgaccagcc tttagccctc ccacctcatg ggcaagccac aagagatgat
    18241 cttccacagg ccccaatgcc tcaagagacc cctctgccac agcatgctca tcgatgaaga
    18301 ggcgcaacgt ccccacggcg ggccaggcga gaggcttttt ccctcgcagg aagcctccgc
    18361 cagaagcgtt atcagcgatg acttcggcga gagtaaagcg atagccggcc cagatcgagt
    18421 ctagcaggtc gatgcccaga tacgccatcg ctatagcctg ctgagcctgg tgaatggctg
    18481 ctgtaggaga aagatcctcg ccgagcagaa tgacaatttc cggctcgaat cgtggctgaa
    18541 tgaagcgact cagcgagaca accggctgtt ctagcaacat gtcatcatag atccgcccat
    18601 agatgggggt agtgatgccc atctgggcct gcttggcagg actcacgaga ccaagcttat
    18661 agcctcgcac aggacggtgt gctcccaggt gctcctgaat acggtacgcc agactcgcat
    18721 cgagatggaa gcgatttcct gctacggtgc actttgctgc acgcgcgctg cgcacttctt
    18781 ccagaagggc agcttcttct ggattgatcg gcccgtccat cattgacctc gcctgtcctg
    18841 gcacgctaag gagcggccag ctactcccca ctgggatcta gaaacctcgt agattacgac
    18901 ccggatctct tccaggggat actccagttc acgagacgct gtctgcgaca gaagctgaat
    18961 cagatcctcc ttctgcgact gggagcgtcc ttcgagcatt gttactttga gcacgagcat
    19021 ataaccccgc ctgttattga tgaactctct cttctgtccc gttccagtcg cctcagagct
    19081 accagatcca gggatcatgg atctgcactt caaggcagcg gaagttgccg ctatagaaca
    19141 gcagggggag gccctcatgg gcttccaggg cctcaaccag gccaatgtag agcgtgtgat
    19201 cgccggcagg atggacctcc ttcacttggc agagcaggtg ggccagggct ttctccagga
    19261 gaggcaactc tgctttccag aggaaggaaa gcgccagatt cttttgcggt cgcccggcga
    19321 agtgctgggc caccgcttcc tgctcactcg acagaatgtt gacagcatag cggcgactgc
    19381 gctctaacca ctctcgcatg cgtgtacgat tgtcgatgga gaccagcacg agcggcggat
    19441 cgagggagac tgacatgaag gcattggcag tcatgccatg cacctgtccc tcgtaagcag
    19501 tggtaatcac ggtcaccccc gtcgcaaagt ggccgagcac gcgacggaac gtgcgaggat
    19561 caaccacgct ctgcggcggc gctgcctcta ctgactgctg ctgcagttcc atgagtgatc
    19621 tcctttctgg cacaagctca cgtctggggc acaggtttgt cttgcgaccg tctggcgagg
    19681 tccagcgcca cgtcgaggat ccagtcctct tgtccagcaa ctgcctggcg gcgccccaac
    19741 tcgaccagaa tactgcgggg atcaagatgg tacagttcac cggcccggcg agcgtgcagc
    19801 aggaaggtcg aatagactcc cgcgtagccg atcgtgagcg catcgcggtc gggcaggggc
    19861 tgatacggag cgataggggc gacaatatac tcggcagcat ccatcagggc gaagacattc
    19921 aggccggggt tctggcccat tttgtcgaga accgcggcga tcacttctgt ggcggcgtta
    19981 ccagctcctg ccccgaggcc acgcaggctg ccatcgatct gatcaacccc agcctccagg
    20041 gcggccagcg tgttccctat agcgagaccc aggttattat gggcatgaaa acccacctgg
    20101 atagtcagcg cctccttgag ggccttgact cgcgcgtagg ccccgtcagg cagaagggcg
    20161 ccagcagaat cgacaatata gacgcaatcg gcgccgtagg attccatcag cctggcttgc
    20221 tcggccagca cctctggagg gcgcatgtgc gccatcatga gaaagcccac ggcttcaagc
    20281 cctagctctt tggtaagacc aaagtgctgc tctgagatat ccgcctctgt acattgggtg
    20341 gcgatgcgga tcacctggac accacgctcg gcagccgctt ttagctcctt gcgtgtcccg
    20401 atcccgggca gcagtaatgc ggcgatcctg gcacgctcgc agacgctgcg cgcctcagca
    20461 atcagctcca gctcaggagt atgagagaac ccatactgca gcgatgctcc tcccaatccg
    20521 tcaccgtgcg tgacctcgat gaccgggacc cctgcccgat ccagggcctg ggcaatgctg
    20581 cgcacctgct ggcgagtaaa ttgatgacgc atggcgtgcg agccgtccct taaagtggta
    20641 tctgtcaggc ggagcgtctt catgccactg cctccttgct tgaaaggaga tgggcggcaa
    20701 agagttcgcc tacgcggcgc gctgcggcgg tcatgatgtc caggttgcca gcataggtgg
    20761 ggagatagtc accggccccg cgcacctcca acagcatcac aatgaccgcg ctcctccccc
    20821 agggagtctc tcgcatctca accacggggg gattcttgag caggtagccc gggacatagc
    20881 gctgcacgtc ggagaccatc tgctcaaccg aggcggccac ttgttggaag tcgaagtcgc
    20941 cctcgggtac cacatacacg gtgttgcgca tcatgatggg cggagaagca ggattgagga
    21001 taatgatggc tttgccctcc cgtgcgccac ccagtgcctc cagtcctcgc gccgttgtgt
    21061 aggtgaactc gtcaatgttc tggcgcgttc caggacctgc cgaagcgctg gccacggtac
    21121 tgaccatctc tgcatagcgc acaggagtca cgcgagagac agcgtagacc agcgggatcg
    21181 tagcctgccc gccacaggtg atcaagttca cattcacctg atccaggtgg gcacccaggt
    21241 tgaccggcgg caccacgtag gggccgcgcg ctgccggcgt caggtcgatg gcgattttcc
    21301 cggcctcacg taagagccgg gcatggcgca tatgcgcttt ggcgcttgtg gcatcaaaga
    21361 caatgcgaat ctcggggtct gcaagaaccg catgaatgct ctcggtgctg gctggtatgc
    21421 caagcgagcg agctcgctcc aacccttctg actgaggatc gatacctgcc accagcgcta
    21481 actccattga ccctggctct ttcaagagct tgtacatcag gtctgtccca atgttgccgg
    21541 agcctaaaat cgcgaccttg attcgcccgt tcgctgctga gtgctgcatg atcgttcagt
    21601 tcctcctctt accagaacaa gctattctcg ggatatagcc gagattctca gaaacctgct
    21661 gagctgctct cacaatggcc agacgatact gctcctcgtt ggcgtagaag cggtaagccg
    21721 gtatcgagag gctcatggcc gcaataacct cgccgctgtg atcacggatc ggcgcggcga
    21781 cacagcaaag ctccaacacg gcctcttcgc gatcgtaggc atatccttgc cggcgcacct
    21841 gctccagttc tgcccgcaat atctgcggat cagtgatcgt gttgggggtg agagctggca
    21901 taccttgctt ctcaaggatt gccaggaccc gctcccaggg cagatgggcc aggatgcatt
    21961 tgccaactcc gctgcagtgg cccggtagcc ttaccccaat cccagtcacg gccacccgca
    22021 ccgcccgtgt cccttgcaat ttctcgatgt agataatctg gtcagattcg agggtagcga
    22081 ggtggacggt ttcaccaaag cgggcgacca gcgattccat caccctgcgg gcctcgctcc
    22141 ggaactcggt ggtctccaga aggagctgag agagcgccac gattcgccag ccgaggcggt
    22201 atcggccggc tggggtgcgc cgtagcagcc ccccttccgt cagactggca gttagcgctg
    22261 aggcggtaga cttggggaaa cccagtttac gcgcgatctc acatactccc cactctggtt
    22321 gctcgtgtga gaagaggttg aggatttgaa acgctttgta gacagcttgc accattgccc
    22381 tgtcctccag gtggacctgt acacagaaaa ccacgtccgt tgtcttccca tccctgtggc
    22441 actgagaggc caacgctctc ctggcatggc cgttgaggaa atcgattgcc ggaatcgctc
    22501 agccaccaag cgcttcccgc cgggcgcata gaatcgctgc cagtgatcgc tcctgctctg
    22561 ttgaaccctc gattgaagta agctagttca agtataagct ggttattttt aacaaacaat
    22621 gcgcccgtcc cgcattgatg gacagatgca ttgactcata tagacagacc agtaccatta
    22681 gtagcgatag tgcagggaag ctttagccgg attccctgcc agtggagtac cacgggggca
    22741 ggggaatgaa ctcctgagcc tggatcagca gcacgactgt ccgtttgtca gcaggaagga
    22801 ggagcttctt tttgcctgat actccctcca ttcaagggtc agtggggtca ggcgagtttc
    22861 aaaaagccca gacaggctcg ctgagggtca agtgattccc aggcgttagt caaccaaaca
    22921 ggtaaaaagg aggaactagc gatggcagta tctcagaatg agccagcccc aggcaaagag
    22981 gccgctgcgg tcccccgtcc gctcactggc gaggaatacc tggagagtct gcgcgacggt
    23041 cgcgaagtct ggatctatgg agagcgcgtc aaagatctca cgactcatcc cgctttccgt
    23101 aatacagctc gcatggttgc acggctctat gattccctgc ataatcctga gaagcagcct
    23161 gttctgacca ccgcaacgga tactggcagt ggcgggtata ctcataagtt ctttgtcact
    23221 tcgcgcagcg ccgaggatct ggtcggggca cgtgatgcta tagcggagtg ggctcgtctc
    23281 tcttacggtt ggcttgggcg cagcccggac tacaaggcca gcttcctggg cacgctcggg
    23341 gccaatgccg agttttatga gccatatcag gagaatgccc ggcgctggta caaggaggcc
    23401 caggagaaag tcctctactt caaccacgcc atcgtcaacc cgccagttga ccggaaccgt
    23461 ccgcctgacg aggttagcga catttacatg cacgtggagc gcgagacgga cgctggcatt
    23521 gttgtcagtg gtgccaaggt cgtagcgact ggttcggctt tgacccatta caattttatc
    23581 gcgcactacg gaccactgcc gatcaagaaa aaagagtacg cccttatctt tgcggtccct
    23641 atggatgcgc caggggtcaa gctcatctgc cggccctcct atgaaatgac ggcggcggtc
    23701 atgggcagcc cgttcgatta tcctctctcc agccgcctgg atgaaaacga cgcggttttc
    23761 atcttcgata aagtgcttgt cccctgggag aacatcttca tttatggaga tgtggagaag
    23821 gccaataact ttttcccgct ctccggcttc atcccgcgct ttaccttcca cggctgtatc
    23881 aggcttgccg tgaagctgga cttcatcgcc ggcctcttcc tgaaagcggt ggaggccacc
    23941 ggcgccaagg acttccgtgg agtacaggcg cgcgtcggcg aggtgctggc ctggcgcaat
    24001 ctcttctggg cgatcaccga tgcgatggcg cgcacgcctc tcgcctggaa caatggggcg
    24061 gtactgccca acaccgacta cgggctggcc taccgcgtct ttgccacggt cgcctacccg
    24121 cgcgtgaagg aactcatcga gaacgacgtc gccagtgcct tgatctacct caattcccat
    24181 gcggtcgatt tcaagacccc cgaggttcgc ccctatcttg aaaagtacat tcgcgggtca
    24241 ggaggctaca gtgccctcga ccgcgtcaag ctgatgaagc tcctctggga tagcctgggt
    24301 agcgagttcg gcgggcgcca tgaactctat gagcgcaact acgcgggcaa tcatgaaaac
    24361 atccgtctgg aggtgctgct tagcgcgatg gcaacggggc aaaccgagca gtacaaagga
    24421 ttcgccgagc agtgcctggc ggaatatgac ctggatggct ggaccgtacc agatctcatc
    24481 aatcccgacg acgttaaccg gcactggaag cagcagagct gacccctgtt cgttgttggt
    24541 cacagttggt ccacaattgg tcacaagctc ggggcgacat cctcgcctct gcaagcagtc
    24601 catgaggagg aaaacaatga gcaacgagca agagcctttg ttcgaagtcg cacagctggc
    24661 tcacgtggaa ttgctgaccc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncatcca gatcgaggcc
    25561 gggccgggca agcatggcat cagccaggcc tactttatgt atgtcttcga gccggggggc
    25621 aatcgggtgg agctgtttgg ggatgcgggc tacctgatct ttgacccggc ctggaagccg
    25681 atcacctggc atgagaagga tctggccaag gggatcatct ggtatggctc cccgctgccc
    25741 gctgagtact tcctctatgg cactccgtcg ccagaagtgg cagctgccct gagccaggcc
    25801 gccagctaga gccttatctt gttcgaatcc atagctcagg gggacctatc cctctcctca
    25861 tccccctgag ctacccccag acctggcaca ttcgaggctg atcttgcccc atgaggagac
    25921 caaggaggta aggaacgatg cccaaattga gtcgtccaga ctactatgac ctgaccagag
    25981 atatgaactg gcgtctcacc tacgtcagcg agaaggaggc ttttccgcca gagctggctg
    26041 gggacgacta cggcattccg ccagctgaat ggtggaagtg ggatgagcca tacaagctca
    26101 cttaccgcga gtatatgcag aaccaggcag agaaggacac gatgtcctac tctgtcaaaa
    26161 gcgcgatcgc tcgctcgcat ctctttgacc aggtagatgc gggctggaag gcagccatcg
    26221 ctgctcacta tggagccatt gcgattcccg agtaccaggc atctttcggg gaagcgcgca
    26281 tggcccgctt tggccgcgca gccgcctgga gaaacatggc aacgtttggc acgctggacg
    26341 agacccgcca cggccagatc cagctctttt tcccgcatga gctactgggg aaaaaccccc
    26401 agtttgactg ggcacacaag gctttccaca ccaacgagtg gggagctatc gccgcgcgtc
    26461 atctcttcga cgatatgttc acagccaatc acgctctcgg tacggcgatc cagctcacct
    26521 tcaccttcga gacagggttt accaatctcc aatttctggg catggcagcc gacgccatgc
    26581 atgtcggcga tgtggagttc ggggccctga tctccagcat tcagaccgat gaggctcgtc
    26641 acgcgcagca aggtgagcct accctcaaga tcctggtcag gaacggcaag aaggccgagg
    26701 cccagcgcct ggtcgacgtc atgttctggc gcagctggcg tatcttctgc ctgctcactg
    26761 gcctctccat ggattactac acgccgttgg agcatcgctc catgtccttt aaggagttca
    26821 tggaggagtg gattatcaag caattcatgg accagttccg tgacctgggc atggataccc
    26881 cctggtactg gaatactttt attgacgagc tagactggta ccatcatggt ctccacctgg
    26941 gagtctggtt ctggcgtccg acggtctggt ggaatcctga tgccggtgta gggccggctg
    27001 agcgcgactg gctggaggcc aagtatcctg gctggaacga cacctggggc aagcggtggg
    27061 acgtgattac cgagaatgcc aggaaggggg aaatcaccaa gaccctgccc cagaccttgc
    27121 cgatcatctg caacctctgc cagattccta ttattacccc cgctaatcct ggaagacagc
    27181 aggagctgga tccctacgtc actgagtatg gcggcagacg ctatctcttc tgttccgagc
    27241 cgtgcaaatg gatctttgac cagaacccgg gccgctatgc cgggcacaag acggtggtag
    27301 atcgttttct ggcaggcgag attcaacctc ccaccctgga aggagcgctg gcctacatgg
    27361 gcgggcctca cctcgtggga gggcgagacg ccgacgattt cgcctgggcc cgcgatccag
    27421 tgctggcagg aacacgctag tgttctcctc ctgtcctcca agggggaggg tggcatggta
    27481 tggtctcagc cagccgccca ccctctcccc accccttcat cagatagatc tacgttcagc
    27541 tggctggtag tagagtagaa aggagagcga tactgtggct ctgattccac tcacagcagc
    27601 gtttcgtgaa gattttgtgg tgcagcttgt gccagttgag gatagcgata ccatcgcaac
    27661 cgtggctcag aaagtggcag cgcacgctgt agggctgcgc gtggccccac gccagtcccc
    27721 catgcaggtg atctttcagg gcaaggtcct ggacccggag aagaaggtgg cagacgtccc
    27781 cctcagccca atggattttg tggaggtacg ctatgccgat ggatgaggcg acgctgacct
    27841 ggatcgaggt ggcaaccctc gatgagcttt gggagggaga gatgctagag gtagaggtag
    27901 atgggcagcc tgttcttctg atgcatctct ctggtggcga aatcgtagca ttccagggcg
    27961 tctgccccca tcaggaatat ccgcttgtcg aaggggagct ggatcttgac accgagacgt
    28021 taacctgctc cggtcaccat tggcagttcc acgctcgaag tggttgcggg attaatcccg
    28081 agggatgtac cctctaccgc tacagcgtca ggttggacgg ctcaacgatt ctcatcggct
    28141 atccgacagg cagcgagagc aggcagcgtt accatcgctg cagagaggag taaaaattgg
    28201 tgagtcgcga acacctcgta ggcccagtgc tcaacgaccg ggctatgttc gaaacggtcc
    28261 tggaagcagc agagattgac aatcctggcg tagcgctcca gacagaggac cgcgagggct
    28321 attttcgcat cgctgcccct cagcgactcc gcctgagccg aaaaagcctg gaggaagtac
    28381 tgggaagacc gttcagactg gcagagctgg agccatacct ttctagcttt ggcgggcgca
    28441 tgcaaatagt cggagaggaa gagttgattt tctaccttga gcgaggagct gagccatgac
    28501 atcctcatcg ccatccacca gacagcagag acgtcagagg agctggagcc tctacaccga
    28561 gcagcgtaag cccagcgagt atgaaatcgt cactcatcgt ctgaattatc attttcggcg
    28621 cgcgccagcc ccgtttgagc tggaccctga catgcccctc aatcgctggt atcttcgcta
    28681 tcgagaaggc tccccgttcc aggtagacga ctgggagggc ttcagagacc ctgcgcgcct
    28741 gacttatcgg gcctacatcc agcgacagcg cgagcgtgaa ctgtacctgg ataaccttat
    28801 cgatgaattt gagcgcagag atcactttgc tttactgaag cctgattggg tgtgctttct
    28861 ggaacacctg tatctcccca gccgtttcgc tggtcacgtg ctgcagatgg tctctctcta
    28921 cgtcagccag atggctcctt cctcctatat tacagtagcc tttcacttcc aaggggccga
    28981 tgagatgcgc cgtgtccagc gctcggccta tgtcgcgaaa gcgctctctc tggcccacta
    29041 tcaggagctc gctgatagcc agaaaaccag agcaatttgg gaaacgaccc ccgcctggca
    29101 gcctgcacgc gagctactcg aaaagctgct gatcgcctac gactggggtg aggctttcac
    29161 cgccctggcc ctggtgacaa agccggtcta tgatgccttc tttaatgagc agcttgggac
    29221 gctggcccgt ctcaacggcg atgacctgct ggccctcatg gctgatgatt tcgcgctgga
    29281 tcagaagcgt caccaggaca ccgcgcgggc cctcgtactc tacgctcttg agcggaagcc
    29341 agagctgcgc accaccctca atggctgggt ggcaaagtgg gcccctgccg ctcaccgggc
    29401 cgtggaaggc ctcgccacat tcttcagctg ggccccgcac ccgctggaaa agaaggcagt
    29461 ggtacaggcc gttggtcagc gtctccagga cttcctgaag gagtgtcagc tctcgctgta
    29521 agcgctgggt cttggcagtt cgctcgcact ctgcgccaaa ggggacccgt gtatgcgagc
    29581 gaactgccag gtaactccct cacaggagga ccagagcagc ccggtggtga cggacatgaa
    29641 gagcagctat ggctatcttc ccccaagcct gcgtcgcctc cccgtcaggc agggcgaaag
    29701 gccattgatt aatccgcgct atccttgcct gcaggataat cattgggccc cacccaggat
    29761 acgggagcag ttcttgcgat ggctggcaaa gctggaaggg gtctcgttag agtcatcgga
    29821 cttaccgcca gctgcccagg ccctcgtact ggcagaacat ctggccaaag gaaagccaga
    29881 ggcatttatc cgcgggaaaa cattcgccat tgttcgtgcg gatggcagtg tccatcttct
    29941 gctggaacca gcgtggggcc aaaaagtgct ggacaagggc tggggaagtg ttcaccccct
    30001 ggcccggtat atggctggag ctattccgcc tcagagcctg atcctttacg ctccccgtga
    30061 tcagcgcgat ctggcagtcc tgagacggat cattctggca gcgtacagct tcgcctgcgg
    30121 gcgcattgga acgcagatct tgccagatac ggcctggtag ccctgggcaa cgctacgagt
    30181 gacaaggagg gtttgacaat gtcagcgact atgaaggctg ctgtctatta tggtccacag
    30241 gatgttcgga tagacgaggt ccctcgaccg gccctcaccg atcctcacgc tatggtggtc
    30301 cgtgtgcgcg ccacttccat atgcggctct gacctccata tctggcgagg aacacttgac
    30361 ggcattatga agcggggcag ttcccggctc ggccatgaac tctcgggaga ggtcgtcgag
    30421 gtaggctcgg ctgtgggggc cttcaagccg ggagatcggg tgactatggc ttactccgct
    30481 tcctgtggag agtgctacat gtgtcgcgta ggacagacag cgcactgcct gacaacgcag
    30541 aaagcggtct atggtttcgg cacggccttc ggcgatctca acggctgcca ggctgaatat
    30601 ctactgattc cttttgcaga tgcccacgcg atgaaggtag cccccgggct ttccgacgag
    30661 cag
//
