LOCUS       NZ_NAQX01000046        22457 bp    DNA     linear   CON 09-SEP-2024
DEFINITION  Dolosigranulum pigrum strain KPL1922_CDC39-95
            NODE_75_length_77128_cov_66.578545, whole genome shotgun sequence.
ACCESSION   NZ_NAQX01000046
VERSION     NZ_NAQX01000046.1
KEYWORDS    WGS; RefSeq.
SOURCE      Dolosigranulum pigrum
  ORGANISM  Dolosigranulum pigrum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
            Dolosigranulum.
REFERENCE   1  (bases 1 to 77266)
  AUTHORS   Brugger,S.D., Pettigrew,M., Kong,Y. and Lemon,K.P.
  TITLE     wgs assembly of Dolosigranulum pigrum KPL CDC strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 77266)
  AUTHORS   Brugger,S.D., Pettigrew,M., Kong,Y. and Lemon,K.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2017) Microbiology, Forsyth Institute, 245 First
            Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. DECEMBER-2015
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003264145.1-RS_2024_09_09
            Annotation Date                   :: 09/09/2024 01:06:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,809
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,695
            CDSs (with protein)               :: 1,695
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 11, 17 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 11, 17 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:30:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 41075
            Orig. end                         :: 63532
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NAQX01000046.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22457
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..22457
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22457
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12457](+)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12457
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     misc_feature    149..167
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.09"
                     /tool="antismash"
     misc_feature    529..544
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: strong, score: 24.72"
                     /tool="antismash"
     gene            1116..2771
                     /gene="treC"
                     /locus_tag="B8A46_RS06260"
                     /old_locus_tag="B8A46_06215"
     CDS             1116..2771
                     /EC_number="3.2.1.93"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA]"
                     /GO_process="GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="treC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Alpha-amylase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1257: Alpha-glucosidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635380.1"
                     /locus_tag="B8A46_RS06260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06215"
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="WP_112789367.1"
                     /sec_met_domain="Alpha-amylase (E-value: 2e-97, bitscore:
                     318.7, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGKKFHDKVVYQIYPKSWKDTTGTGMGDLQGVIEKLPYLADLGID
                     LLWLNPFYKSPQNDNGYDIADYKSIDPMYGDFDTFDKLVQEASKYNIGLMLDMVLNHVS
                     TEHEWFQRALAGEEQYQDYFILRQAQPDGSLPTNWESKFGGPAWNQLGDSDYYYLCLYD
                     KTQADLNWHNPEVREALYDVVNFWINKGVKGFRFDVLNVIGKSQDLKDAADGVGKKEYT
                     DTPIVHEWVRELNKRTFGRYDDIITVGEMSSTDVSNSVQYSHSESNELDMVFSFHHLKV
                     DYKNGDKWTLMDFDFDELKRLLDHWQTGMQAGNGWNALFWNNHDQPRSNSRFADPVNYP
                     YESATMLATTIHLLRGTPYIYQGEEIGMTNPNYESIDQYDDVETHNAYRTLLSQGMNNS
                     EALAIIQAKSRDNSRTPMQWTSEDQAGFTTGQPWLEVASNYKYINTNVTDPSQHIRDYY
                     KQLIRLRKDYAVISEGTYRSIQLDHPDVYSYMREYDNQQVLVFNNFYGKQTAIDVPEDF
                     LKKDARILIGNYDQHALEQQLVLQPYESIAFLLE"
     gene            complement(2818..3378)
                     /locus_tag="B8A46_RS06265"
                     /old_locus_tag="B8A46_06220"
     CDS             complement(2818..3378)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014669.5"
                     /locus_tag="B8A46_RS06265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06220"
                     /product="biotin transporter BioY"
                     /protein_id="WP_111949520.1"
                     /transl_table=11
                     /translation="MRTLTTKDLVYIAVLTAMLCVSSLVAIPVGIGIPITLQVFFWLLI
                     PALLKAYRGFLSLALYVLIGLIGIPVFAGGTGGFQAVLSPSFGFLLGSLIIALYIGKVA
                     QKRPSLLTMILHMIVAILILYTIGILYQYFIFNVIAESGGSTTLISLIIANVSSFLPLD
                     ILKAILAGIVYDRLIRHTRFKNI"
     misc_feature    complement(3399..3412)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: strong, score: 21.97"
                     /tool="antismash"
     gene            3550..4194
                     /locus_tag="B8A46_RS06270"
                     /old_locus_tag="B8A46_06225"
     CDS             3550..4194
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635382.1"
                     /locus_tag="B8A46_RS06270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06225"
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="WP_112789368.1"
                     /transl_table=11
                     /translation="MTPNKENFLKAIYELGGMSKLINNKSLAEYLNVSAAAITDMNTRL
                     VKQSIITYEPYKGVKLTDKGVRIVNQLIRRHRLWEVFLAENLGYEWDEVHTDADLLEHI
                     SSDKLIERLDAFLGHPTVDPHGDTIPTSDGKVIVNQYHALVECKQGESFKVKQVDDDTE
                     FLTYLTDKGIQLNETYQITEIEPYEGPITLTNNDEENILVSYKAAFRIFGQ"
     CDS             4241..4372
                     /gene="allorf_45316_45447"
                     /locus_tag="allorf_45316_45447"
                     /protein_id="allorf_45316_45447"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MVFFLVVREQIIYCVLSKIYNPMTKGGRYCSWDVINCYRAVIE"
     CDS_motif       4241..4282
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MVFFLVVREQIIYC"
                     /locus_tag="allorf_45316_45447_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       4283..4372
                     /prepeptide="core"
                     /locus_tag="allorf_45316_45447_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-6.90"
                     /molecular_weight="3325.9"
                     /monoisotopic_mass="3323.7"
                     /core_sequence="VLSKIYNPMTKGGRYCSWDVINCYRAVIE"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MVFFLVVREQIIYC"
                     /leader_location="[4240:4282](+)"
                     /alternative_weights="3343.9"
     gene            4472..5773
                     /locus_tag="B8A46_RS06275"
                     /old_locus_tag="B8A46_06230"
     CDS             4472..5773
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B8A46_RS06275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B8A46_06230"
                     /product="hypothetical protein"
                     /protein_id="WP_004635383.1"
                     /transl_table=11
                     /translation="MKLKNILKTASILALLTGPSATMTVDHVYAEEAQDKVADSETNLE
                     DLRQQVRSSVQTIEDLNNELVQIDETIDGIEAEIAQTEEDISQQEAIIQDYFDQAKSRL
                     QNMQLSNVNENAVLSLLEAESFQDIIARVNAVVQLTQANSDQIQSLQAQKDELDQLVQS
                     LETKQTDLSNKKETIDAKKQSVHTEIADLERLIEENREDFETLKEESEIVEICRRAEEL
                     VEERQAEQEVQEDASSNEEAKQVASQSAEETQQPQAEETVAIEENEEPAEPTDDSAAEQ
                     SVEEPQVTETEPAEAEQSQPEPETQKQPDQQVQEESQTQTEPEVQEEPIQPQAAVPTTA
                     QYSIDDLEFQGVINALGKKWTFYSERVLPGGGLNIPGRHTADGFVRDGEGYIVLAASSS
                     VGHGTIIDTPFGSQGKVYDTCASCHAGWFDVYTR"
     CDS             5815..5892
                     /gene="allorf_46890_46967"
                     /locus_tag="allorf_46890_46967"
                     /protein_id="allorf_46890_46967"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MRVAMTGSFSYRHICCGVFLINKIM"
     CDS_motif       5815..5838
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MRVAMTGS"
                     /locus_tag="allorf_46890_46967_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       5839..5892
                     /prepeptide="core"
                     /locus_tag="allorf_46890_46967_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-5.70"
                     /molecular_weight="2026.5"
                     /monoisotopic_mass="2025.0"
                     /core_sequence="FSYRHICCGVFLINKIM"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MRVAMTGS"
                     /leader_location="[5814:5838](+)"
     gene            6476..7393
                     /locus_tag="B8A46_RS06280"
                     /old_locus_tag="B8A46_06235"
     CDS             6476..7393
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635384.1"
                     /locus_tag="B8A46_RS06280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06235"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_004635384.1"
                     /transl_table=11
                     /translation="MYYMKNVIEINNLTKSYDNVAVIKSININIVEGTIHALLGKNGAG
                     KTTILKSILGLIKPTSGSIFILGENQLTEKGRNVLKQVGCMIETPGFYPNLTGTENLSI
                     FAKLRELDKQAVQEALTIVNLPYADSKKFKEYSLGMKQRLAIANAIMHDPDILILDEPT
                     NGLDPSGVIEIRELCKEMKRQGKTLLISSHILSEVEQVADEVSIIDEGELVKYINLKKM
                     NENSQVTIHIFTSNFDKVKSVLLEAGVSQHNMINTAKGVRLLSKDMDISEVNKLLTQND
                     VDIEGIMREKLTLEEQFQRVTETR"
     gene            7398..8132
                     /locus_tag="B8A46_RS06285"
                     /old_locus_tag="B8A46_06240"
     CDS             7398..8132
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024141.5"
                     /locus_tag="B8A46_RS06285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06240"
                     /product="ABC transporter permease"
                     /protein_id="WP_004635385.1"
                     /transl_table=11
                     /translation="MLDLVSTEITKITRMKQMWVYASVLGIYITMMSVYTAQAVGLFDQ
                     FIHLYKFSLSYTGFLLLPFILLSLSSSTISDDYRNEVMKNLTVIPISKQKIIAAKLIAI
                     YIVLSVAMVMIWISVCVIGYVTRFRFSMTWLLIFRYLWLCILTSLVIFLSMLPVTLISV
                     ATKGNVVITNLIGSMYIIASFFLTNFMNGIIPLATAPHIIWYGSMEGVEVNSNIALMVI
                     SIIFYTIIVLFAMNKILKKQEL"
     gene            8152..8868
                     /locus_tag="B8A46_RS06290"
                     /old_locus_tag="B8A46_06245"
     CDS             8152..8868
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024141.5"
                     /locus_tag="B8A46_RS06290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06245"
                     /product="ABC transporter permease"
                     /protein_id="WP_004635386.1"
                     /transl_table=11
                     /translation="MVLEFKKMKRSKLIIVLILIVVLNIGIHYLMGNVKYVGIPYAAEP
                     GWTLQNTLIVGTYYLLLPVFTLIGSASFIIERENSVYINILTIPVRKSHLILNKSKFIW
                     VVSMIFTSSIFVFTLVLEKIHSNILTQEIVLKYLFEYVVHSNGLYVIAMLILSVIIYMD
                     YNMQLAVMVGFVSSFVSVFIEQTAISYLYPVNALFNISGFKESNLYEYSSSVVILCVIA
                     ICAVFIYKKIAQQESW"
     gene            8917..9144
                     /locus_tag="B8A46_RS06295"
                     /old_locus_tag="B8A46_06250"
     CDS             8917..9144
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167060.1"
                     /locus_tag="B8A46_RS06295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06250"
                     /product="plantaricin C family lantibiotic"
                     /protein_id="WP_004635387.1"
                     /transl_table=11
                     /translation="MKNKVNNWKTPVYEKEEDYDNPAGDIFRELKSDDIDKVMASGTAN
                     TYCRCYSGRHSCGRACTITAECPVFTVACC"
     CDS_motif       8917..9042
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MKNKVNNWKTPVYEKEEDYDNPAGDIFRELKSDDIDKVMASG"
                     /locus_tag="B8A46_RS06295_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       9043..9144
                     /prepeptide="core"
                     /locus_tag="B8A46_RS06295_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-5.60"
                     /molecular_weight="3422.9"
                     /monoisotopic_mass="3420.5"
                     /core_sequence="TANTYCRCYSGRHSCGRACTITAECPVFTVACC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKNKVNNWKTPVYEKEEDYDNPAGDIFRELKSDDIDKVMAS
                     G"
                     /leader_location="[8916:9042](+)"
     gene            9231..9974
                     /locus_tag="B8A46_RS06300"
                     /old_locus_tag="B8A46_06255"
     CDS             9231..9974
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B8A46_RS06300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B8A46_06255"
                     /product="hypothetical protein"
                     /protein_id="WP_004635388.1"
                     /transl_table=11
                     /translation="MKLIDKFKNGLYSFERDIQHDETNNYKSENRLQYWKKFLGVNERE
                     IENILSNGLGINTANLNELLSENDNFSCKVTETNVLWNQLIHDLQVLSIESIILPEFYI
                     IGDIGQKELPMFYGFHEPFLKLAILRFENYWKNIPGISDNVFNKLLIYLYDQLAEISYR
                     TLILELNIAREENKLAGETSEERYNYFSTQYLSDNYWLILEEYPVMFRLMCEATQKWIN
                     NTTRFIDRILSDKDDLEKLLKLREN"
     gene            10001..12457
                     /locus_tag="B8A46_RS06305"
                     /old_locus_tag="B8A46_06260"
     CDS             10001..12457
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /locus_tag="B8A46_RS06305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06260"
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="WP_244325507.1"
                     /sec_met_domain="DUF4135 (E-value: 1.5e-91, bitscore:
                     299.5, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 1.2e-65, bitscore:
                     213.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHNGGQTVIILHFEEGTSVVYKPRDLKMDVTFQSTIKWFNEVTKS
                     HLYSLKIINHTEYGWVEYIPHEECKDYSDFKNYYTELGQLLFLFYLLRGNDIHYENIIA
                     KGKHPVLIDLETLFHNNTSNTSGIDTAADRVNELLENSVRTVGILPNLVWAQNGKNGVD
                     ISAISTSENKEIPIEQASITNVNKDNMKVEYKTSTLASQKNNPYIIGEEISLTSYHKYL
                     KKGFIESYTKIKNINKKEIINQVENYKEIYARQILRPTQYYTTLIQISLHPDFLRSAID
                     REMLFSKLWIYFDENNSFRKVSEIEFTSLLKNDIPWLISNVSKNNITTKDGSEIESIFK
                     HSSIALVKEKINILGDKDLTLQVELIETALNYDSEYNKAESQRENDRKIIEINDDKLNK
                     NHLDQQLLEISTNIGDYLINQSFIGMNGDVSWIDMNVIGEKANDWNMVPTSMDLYSGLS
                     GIMIYFIFLYKETKQNKYLIMVKRCYKSIINYIKNVRKRTNINSEVMFGGFSGETPIIY
                     ALTILEEELGGIFDLDELEKIRSWIFKECKKNISVGNEHDIIIGSSGVIAILLRYYDLT
                     SNDAILEVCQQYAEQIIDNYIEMDNNSIAWIGIASRNALGGFAHGVSGIVWALSKLYSY
                     LPDERYIEVIEKALRYEDYLYSEDDKNWVDRRETEEGIEYNNLSSNMPVAWCHGASGIL
                     LSRASLKKHNLPLSEKRKNKIDEDIEIAVRTTLKNGFGHSHCLCHGDLGNMLILKYASS
                     ELNTKNDIDKRYDIYMSHLISQLKDKWECGIPYKNSPGMMLGLSGIGFGLLSLMNEDLP
                     FILLLE"
     gene            12529..13236
                     /locus_tag="B8A46_RS06310"
                     /old_locus_tag="B8A46_06265"
     CDS             12529..13236
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015377.5"
                     /locus_tag="B8A46_RS06310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06265"
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_181453638.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.7e-36, bitscore:
                     116.1, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIKFVQQLSESDCGIAALTMVLNYYGCKLHISDLVEKCSISRD
                     GVHLRDLMNVANEYGLKSKAVELNKKENIFSEKIVFPCIAVLSMSHYVVIEKAKRGTIY
                     YLDPECGRIIMDGSEFSKKFTGILLLFFPENIKKQKSQNKIIEILTLGEIKKKFIVGVI
                     FFSFIIQLFVLLMPLFTEYMIDNVINGIALVSHVKLIIGILFAILSYGVFSFVRELLTT
                     LLEIKYISTLKKM"
     gene            13236..14006
                     /locus_tag="B8A46_RS06315"
                     /old_locus_tag="B8A46_06270"
     CDS             13236..14006
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.5"
                     /locus_tag="B8A46_RS06315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06270"
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="WP_112789370.1"
                     /transl_table=11
                     /translation="MRKLFYLPLSFFDVRSSGDIVSRINNIDSIQQLLSNIITGVFVDV
                     LTIVISISMMLYISRILSIIMIVYGILLCVLLNIFFKKTDVKNKLSLMKREKTQSYLIE
                     LSSNINMMKTSNYGEALYNKWVKNYNEQMHLEFKRQRLLGMYRSLIISYRLLPSVTVLF
                     IGSNFVNQGMLTLGQVMSFLALGNTLLSPLAILIQNIFDFQYSKNNIDRLSEIITAKSE
                     KNFDGREINSFKNIKFQDVNFSYSGINGKKMLKS"
     gene            14033..14644
                     /locus_tag="B8A46_RS06320"
                     /old_locus_tag="B8A46_06275"
     CDS             14033..14644
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.5"
                     /locus_tag="B8A46_RS06320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06275"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_256998287.1"
                     /transl_table=11
                     /translation="MSFVGRTGCGKTTIIKLILSLYNNFEGNILLNNIDKEVYDLKSYR
                     QLFGTVLQDETLLNDTIQKNIDPTHSHTISKIREAAKLACLDEDVMNRMPSKYNTEIGD
                     NGRNLSGGQRQRVAIARALLTNPKVIILDEGTSQLDVSTEREIFNNLKKKNITIIAVTH
                     KLSTTTISDCVYVLKKGKIIAHGKHEKLMQNNRYYSDFFR"
     gene            14925..15329
                     /locus_tag="B8A46_RS06325"
                     /old_locus_tag="B8A46_06280"
     CDS             14925..15329
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014608.5"
                     /locus_tag="B8A46_RS06325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06280"
                     /product="OsmC family protein"
                     /protein_id="WP_112789371.1"
                     /transl_table=11
                     /translation="MLYEVVAVNETGIDGQSYLVDGESYQTSSPTSDEPGTNPEQLMGL
                     SLATCFNATLHAVLKERGLEPESRVQVTVQLHQDEENKEYYFTLDLEAAVDKIDLQEAK
                     EIVEVAHKRCPVAKIVGNYEGMTVKTVPFN"
     gene            15431..16453
                     /locus_tag="B8A46_RS06330"
                     /old_locus_tag="B8A46_06285"
     CDS             15431..16453
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1066: alpha/beta hydrolase domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF019474.5"
                     /locus_tag="B8A46_RS06330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06285"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_146737342.1"
                     /transl_table=11
                     /translation="MGYYWADVVIRFTRIFSKLHQRFKRRRIKPAKQANVYKNIPYKSN
                     YPNNKLDIYVPCESSSVHPTPELTDQKQEKDPSNPNVVEDCQTEMFDVLKPVIVYFHGG
                     GFAWGDKIDERRYLLEFVKAGYAVVAANYALTPMYKYPVPIIQASEVMDFLVNEGEQYG
                     LDPDNIVLAGLSAGGHLAGQLALAESSSAYADRLELSQHPDLTIRGIMLNSALIDPNRS
                     NQVGHWLINWLFSVMGFSYMDMNPRNMKEQMLAEANLLNHINEALPPVYISDGNRVSFT
                     QQATDLVAQLRARDIYVQSNIYTDDYPGFMFHGYEGQLWLREARENVDKQLDFLETVID
                     "
     gene            complement(16761..17456)
                     /locus_tag="B8A46_RS09215"
     CDS             complement(16761..17456)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B8A46_RS09215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_172763308.1"
                     /transl_table=11
                     /translation="MLNKIKKVATFILTLGLMISVITPNRVIASENQYEATSLKKYEDL
                     RIIQSEELRTDAVNKFNSLFQNGKIEYSEEYFDFDNIKIAEMNGEVYATISKNDSVLST
                     VTIPLNTNENFYVENYFYEKDGNFWIKTKQDGEVISDVDSEIQFVNNDEILQGIRELND
                     YAIQSQQRGLNVSCFLAVSGAGATVSGLIAKVCGTPCVLAPPTCIVCLGGIVVVGGGSI
                     AAAVWKCWE"
     misc_feature    complement(17520..17536)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.97"
                     /tool="antismash"
     misc_feature    17543..17557
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.58"
                     /tool="antismash"
     gene            complement(17573..17803)
                     /locus_tag="B8A46_RS06340"
                     /old_locus_tag="B8A46_06295"
     CDS             complement(17573..17803)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182144.1"
                     /locus_tag="B8A46_RS06340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06295"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_077863001.1"
                     /transl_table=11
                     /translation="MKNRIKEFRKPLGLSQHRLGKKVGVSRVSINKIETGKTIPNLKLA
                     SDIADALNTSIYKVFDLDGEENYCYCDNQSL"
     CDS_motif       complement(17639..17803)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MKNRIKEFRKPLGLSQHRLGKKVGVSRVSINKIETGKTIPNLKLASDIADALNTS"
                     /locus_tag="B8A46_RS06340_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(17573..17638)
                     /prepeptide="core"
                     /locus_tag="B8A46_RS06340_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-7.00"
                     /molecular_weight="2513.7"
                     /monoisotopic_mass="2512.1"
                     /core_sequence="IYKVFDLDGEENYCYCDNQSL"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKNRIKEFRKPLGLSQHRLGKKVGVSRVSINKIETGKTIPN
                     LKLASDIADALNTS"
                     /leader_location="[17638:17803](-)"
     gene            18185..18886
                     /locus_tag="B8A46_RS06345"
                     /old_locus_tag="B8A46_06300"
     CDS             18185..18886
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628236.1"
                     /locus_tag="B8A46_RS06345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06300"
                     /product="metallophosphoesterase family protein"
                     /protein_id="WP_112789373.1"
                     /transl_table=11
                     /translation="MDKSYIFAVSDVHGSDEQLEALLEYWNPEEEQLVILGDLCDRGPD
                     AQAVFRRAKQLKEEYGAICLRGNHEDMLMKFLDDPADNVAHYYRNGGEITLESFLGDAV
                     SSSTPQELAEALKDQYPWLIDYLDNLELTYEWGKYFFVHAGMDLTLDDWRESTVRDKIW
                     IRKGFLDVPNETEKTFVFGHTPTMKLRTDGQPDLWVSEDHKIGIDGGAVYGGQLNGVKL
                     SQAGIEATYRA"
     gene            18920..21106
                     /locus_tag="B8A46_RS06350"
                     /old_locus_tag="B8A46_06305"
     CDS             18920..21106
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049800.1"
                     /locus_tag="B8A46_RS06350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06305"
                     /product="Tex family protein"
                     /protein_id="WP_373853654.1"
                     /transl_table=11
                     /translation="MISVEDKQTFQLIVRELSDYSTKQVRTVLSLLDDGNTVPFIARYR
                     KDQTGALDEVQIREIQQRSEYIQNLQDRKETVLSAIEEQGELTDELAAEIREATQLQEV
                     EDLYRPYKKKRQTLATKAKEAGLEPLADWLLGFPDASEAEIEAIASDYLNSEYDVETVA
                     DALAGAHEIIAEQVADNPEYRKRLRDYTIYNAQITSTVKNGEIDEKNVFQQYYEYAEDY
                     RKMAPHRTLALNRGESEEVLTVKLEVGDERAHRYLYKQILPSLDSSSPVISIVKEAIKD
                     AYKRFIAPSIERELRGTLTEKAETHAIQIFGENLKNLLMQAPLKQKTILGLDPAYRSGC
                     KLAVIDSTGKVLAIKVIYPHTSGETKRNQSKEALKQLITEHAVDVIAIGNGTASRESEQ
                     FVAEVVSEMPEKTAFIIVNEAGASVYSASDEARREFPDLAVEERSAISIARRLQDPLAE
                     LVKIDPKSIGVGQYQHDVSQKELEGQLDFVVETAVNQVGVNLNTASGALLEHVSGLTKT
                     AANNIVAYRESEGQFTTRDQIKQVKRLGPKSYQQAVGFLRIPDAPEPFDQTGIHPESYT
                     EAEKILAHVKRDKSAIGSDELKHLLEQLSDVQTAEQLEIGLETYRDIVAALIAPGRDAR
                     DDMPAPILSTDVLKMDDLREGMQLQGTVRNVVDFGAFVDIGVKEDGLVHISELSDQFVE
                     HPKDVVQVGDIVTVWIKSIDLDRSRIGLTMIQKD"
     gene            21131..21610
                     /locus_tag="B8A46_RS06355"
                     /old_locus_tag="B8A46_06310"
     CDS             21131..21610
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635404.1"
                     /locus_tag="B8A46_RS06355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_06310"
                     /product="SprT family protein"
                     /protein_id="WP_112789375.1"
                     /transl_table=11
                     /translation="MKLIRTNDSLQAYVEAVSEEWFHHPFRHRATFNSRLRTTGGRYHL
                     QTHHLDFNPKILDVFGEAVFLGIVKHELCHYHLHIAGRGYQHRDADFRKLLKEVGGLRY
                     TPSIEQQKGVAKRWHYQCSRCGQSYYRKRRVNTSSYCCGRCRGKLKLFEQVICAR"
     misc_feature    22330..22346
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 21.17"
                     /tool="antismash"
     misc_feature    22367..22381
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.73"
                     /tool="antismash"
ORIGIN
        1 attgttgaag tgtccccatt ctggaatggg gtaaatcatt tcctatggtt accgggtgaa
       61 gctattttcc acttcttacc ggtcggaatt acatgggcag ttacgcgtaa gatggggact
      121 actcaaattt taggaattgt cttgggaatt acgttagtat caccgcaact tttaaatgca
      181 tacggtgtgg aggaaatttt agctggtgat gtccagcagt gggactttgg gtttttccaa
      241 cttgatatga ttggctacca agctcaagtt attcctgcga tgttagccgg atttacgctt
      301 gcatatctag agcgattttt ccgtaaaatt acaccagaag cgatttcaat gattgttgtg
      361 ccattctttg ccttagtgcc cacaattttt attgcccatg ctgtgattgg tcccattggt
      421 tggcagcttg gtaagtggtt agcggacggg gtacagtggg gtcttagcgg ttccttgaat
      481 tggttatttg gcttcatctt cggtggattg tatgcaccgt tcgtcgttac aggtctacac
      541 cacatgacga atgctattga cttacaacta gttgctgagt atgatcagac aattctgtgg
      601 ccaatgattg ctttctccaa tattgcacaa ggatcggcag tattagctat ttggtggaag
      661 aaccgtcata atgaaaaaga aagtgcagtg tcattaccag ctgcaatttc agcttattta
      721 ggtgtgactg aaccggctct tttcgggata aatattaagt atgtttatcc actcgtagca
      781 ggaatgatcg gatcagcgtt tgcaggaatg tactcggtag cgacaagcac cgcagcgtat
      841 acgattggtg tcggtggatt gccgggaatc ttgtcagcta caaatagttc ctatttgaat
      901 ttcggaattg ccatgatgat tgccttcttt ataccgatta tacttgtttt tatctttgag
      961 aaatataatg ttttaaccga tcaacaatta gaaggactac ctattccgaa gttgggaagc
     1021 taggaaatta aagatagttc agtgtaaagt actttgataa agtgattgca taattaattt
     1081 atcgaagtac ttttttattg agaaaggatg ataccatggg gaaaaaattt catgataaag
     1141 ttgtctatca aatctatcct aagtcttgga aggatacaac tggaacagga atgggtgatc
     1201 tacaaggggt tattgaaaag ttaccgtatt tagctgattt aggcattgat ttgttgtggt
     1261 taaatccctt ttataaatca cctcagaatg ataatggcta tgatattgca gattataaat
     1321 caattgatcc aatgtatggg gattttgaca cctttgacaa acttgtccaa gaagcgtcta
     1381 aatataatat tggcttaatg ctcgatatgg tgttgaatca tgtttcaaca gagcatgagt
     1441 ggttccaacg agcacttgcc ggcgaagaac agtatcaaga ttacttcatt ttgcgtcaag
     1501 cgcaaccaga tggcagttta ccgacaaatt gggagtcaaa atttggtgga cctgcttgga
     1561 atcaattggg agatagcgac tattattatc tttgtttata tgataaaacc caagcagatt
     1621 tgaactggca taacccagaa gtaagagaag cattgtatga tgtggttaat ttttggatta
     1681 ataaaggggt aaaaggtttt cgcttcgatg tactcaatgt tattggtaaa agtcaagatc
     1741 taaaagatgc tgctgatggt gtggggaaaa aagaatatac agatacgccg attgtccatg
     1801 agtgggtgcg agagctgaat aaacgtacct ttggccgata tgatgatatc ataacagttg
     1861 gtgagatgag ttcgacggat gtatccaata gtgtccaata ttcccattct gagtcaaatg
     1921 aattggacat ggttttcagt tttcatcatc taaaggtgga ctataaaaat ggagacaaat
     1981 ggactttgat ggattttgat tttgatgaat tgaagcgttt attggatcat tggcaaacag
     2041 gaatgcaggc gggcaatgga tggaatgctt tattttggaa taatcatgat caaccacgat
     2101 ctaatagtcg cttcgctgat ccagttaatt atccgtacga atcagcaacg atgctagcaa
     2161 caacgattca tttactacgt ggaacacctt atatttatca aggagaagag attggtatga
     2221 ccaatccaaa ttatgagtcg attgatcaat atgatgatgt agaaacacat aatgcatata
     2281 gaacgctttt gtcacaaggg atgaataatt cagaagcgtt ggccattatt caagcaaaat
     2341 cgcgtgataa tagccgtaca ccaatgcagt ggactagtga ggaccaagct ggcttcacga
     2401 caggtcaacc ttggctagaa gttgcttcta attataaata tatcaataca aatgtgactg
     2461 atccgagtca gcatatcaga gattattata agcagttgat tcgattacgg aaagattatg
     2521 cggtgatatc agagggaaca tatcgtagta ttcagttaga tcatcctgat gtttatagtt
     2581 atatgcgcga atatgacaat cagcaagtac tagtatttaa taatttttat ggaaagcaga
     2641 cagctattga tgtaccggaa gattttttga aaaaagatgc acgaatactg atcggtaatt
     2701 atgaccaaca tgcgctagag caacaacttg tactccaacc ttatgaatca attgcttttt
     2761 tgttagaata gagaaaaaaa aagaacgcta tgtacttagt agcgttcttt tactaattta
     2821 tatgtttttg aagcgagtat gacggataag tcggtcataa acaataccgg caaggatagc
     2881 ttttagaata tcaagtggta agaaagatga aacgttagca ataatcagac tgataagtgt
     2941 cgtagagccg cctgattcag caatgacatt aaaaatgaaa tattgataaa gaattccaat
     3001 cgtatataaa ataagaatag ccacaatcat gtgtaaaatc attgttaata gagagggacg
     3061 tttctgtgca accttcccaa tgtataaagc gatgataagt gaaccaagaa ggaatccaaa
     3121 tgaaggagaa agaacagctt gaaagccacc agttcctccc gcaaatacag gaataccaat
     3181 caatccaatt aatacataaa gagctaagct taaaaatcca cgataagctt taagtaatgc
     3241 aggaataagt agccagaaaa agacttgtaa tgtaatggga ataccgatac caacgggaat
     3301 agcgaccaat gatgatacgc aaagcatagc tgttagtacg gcaatataga ctaaatcctt
     3361 tgttgttaga gttctcatta ttatctcctt ttgctttatt agattatttc tagaaatagt
     3421 atactatatt ttaatttgat attcaaacta gttcctgaaa aatttaatta aaaagatctc
     3481 atgtctctac tacgcattaa gagctatttc atgtataatg aaaaaaagta atgggggaag
     3541 gggacagaaa tgacaccaaa taaggagaat tttttaaagg ctatttacga actaggtggc
     3601 atgtctaaac tgattaataa taagagtttg gcggaatact tgaatgtatc ggctgcagcg
     3661 attacggata tgaatacgcg cttggtgaag caatcaatta ttacatatga accttataaa
     3721 ggggtaaaac tgacggataa aggggttcgt atcgttaatc aattaatacg gcgccatcgt
     3781 ctatgggaag tattcttggc ggaaaattta ggttatgagt gggatgaagt gcatacagat
     3841 gctgatttat tggaacatat ttcttcggat aagttaattg agcgcttaga tgctttccta
     3901 ggacacccaa cagttgatcc acacggggac accattccaa cctcagatgg aaaagtcatc
     3961 gttaatcaat atcatgcttt agtagagtgc aaacaaggtg aatctttcaa ggtgaaacaa
     4021 gtcgatgatg atactgaatt tttaacttat ttgacggata aaggaatcca gttgaacgaa
     4081 acgtatcaaa ttacggaaat tgaaccgtac gaagggccca tcacgttaac gaataacgat
     4141 gaagaaaata ttttagtcag ttataaagcg gcatttcgta tttttggtca ataaataaat
     4201 catcacaatt gagtcagaga ataccaattc tgatacgatt gtggtgtttt ttcttgtagt
     4261 tagagagcag attatctatt gtgtcttgtc taaaatttac aatccgatga caaagggtgg
     4321 ccgatattgt agttgggatg taataaattg ttatcgagct gtcatagaat agatcggttc
     4381 tcctgttata atggtaggtg tcgattggaa gacattatgt tcaatcatgt aatcattata
     4441 tagaattata tagatgagga gcatcttaac tatgaaattg aaaaacattt taaaaacagc
     4501 ctccattttg gctttattga caggaccgag tgctactatg acagtagatc atgtttatgc
     4561 agaagaagct caagacaaag tagcagattc tgaaactaac ttagaagatt taagacaaca
     4621 agttcggtca tctgttcaga cgattgaaga tttgaataat gaacttgtcc agattgatga
     4681 gacgattgat ggcatcgaag cagaaatcgc acaaacagaa gaggatattt cccagcaaga
     4741 agcaatcatt caggactact tcgatcaagc taaatcacgc ttacaaaata tgcaattatc
     4801 taatgtgaat gagaatgctg tcttatcact tcttgaagct gaatcattcc aagacatcat
     4861 tgctcgcgtt aatgcggtcg ttcaattaac ccaagctaat tcagatcaaa tacaatcact
     4921 gcaagcacaa aaagatgagc tcgaccaact agtgcagtca cttgaaacga aacaaacaga
     4981 tctttctaac aaaaaagaga caattgacgc aaaaaaacaa tcagttcata ctgaaatagc
     5041 tgacttggaa agattaattg aagagaatcg cgaagatttc gagacgttga aagaagagtc
     5101 agagatagtt gagatttgtc gtcgggctga agaactagtt gaagaacgtc aagctgagca
     5161 agaagttcag gaagacgcat ctagtaatga agaagctaag caagttgcaa gtcaatcagc
     5221 agaagagacg caacagccac aagctgaaga gactgttgca atagaagaga acgaagaacc
     5281 ggctgaacca actgatgatt cggcagctga acagtcagtg gaagaacctc aagtgacaga
     5341 aactgaaccg gcagaggctg aacaatcaca accagagcca gagacacaaa aacagcctga
     5401 ccaacaagtg caagaagaat cacaaacaca gacagaacca gaagtacaag aagaaccaat
     5461 acaaccacaa gcagctgtcc caacaactgc tcaatattca attgatgatt tagaatttca
     5521 aggggtcatt aatgcacttg ggaagaaatg gacgttctat tcagaacgtg tcctacccgg
     5581 tggcggattg aacattcctg gtcgtcacac agcagatgga ttcgttcgcg atggtgaagg
     5641 ctatatcgtc ttagctgcca gcagtagtgt cggtcacgga acgattattg atacaccatt
     5701 cggcagtcaa ggaaaagtat acgatacgtg cgcaagctgt cacgctggtt ggttcgacgt
     5761 ctacacgcga taaaataata taatacatgt atagatacca catcgattgt tgtcgtgaga
     5821 gttgctatga caggcagctt ctcctatcga cacatctgtt gtggtgtttt tctaataaat
     5881 aaaattatgt gatttctcaa ataatgtggt aagaccaatt taaatgaaaa aatgagaata
     5941 ttccaagcta ctcatttccc atgtactaga tgcttcgttc gtcttgaaga agaactcgat
     6001 aacatcaata agcaattgga cttttaaata cgattataga ggcatattct gaggcaattg
     6061 actattgata atgtctaaca actccttgaa tgacgtacaa cagcacgtca cttggggtat
     6121 tttttgcact ttgtgatttt tttgtgaaat tttagtttta aaattgtata caattaaata
     6181 atatggtaca atttaatcat taataagtat tcgctttcat atgtatttta gaactgagaa
     6241 actcacaagt gaaaaatatt attttttgat ttatatacag tagttaagat aagtgaggat
     6301 agagcataag gtaatatatc aatttggtga agtgttgaga ttaatgataa ggtcaaaagg
     6361 aaatattttt aatgacaggt atatatgtat aacaatttgt gctaataatc catgaataag
     6421 atataaattt ttaacttaat ttaaatttga gaggagtgag taacactttt gtgttatgta
     6481 ctatatgaaa aacgtaatag aaattaataa tttgacgaaa tcgtacgaca atgtagctgt
     6541 tattaaatca attaatataa atatagttga agggactatt catgctttac ttgggaagaa
     6601 tggcgctgga aaaacaacaa tactgaaaag tatattaggc ttaattaaac caactagtgg
     6661 aagtatattt attctaggag aaaatcaatt gacggaaaaa ggaagaaatg ttctgaaaca
     6721 agttggttgt atgatagaaa caccgggttt ttaccctaac ttaactggaa cagaaaatct
     6781 gtcaattttt gcaaaactta gagagttaga taaacaagca gtacaagaag cattaactat
     6841 agtaaatctt ccatacgcag attcaaaaaa attcaaagaa tattctttag gaatgaaaca
     6901 acgattagct attgcaaatg cgattatgca tgatccggat atcttaattt tggacgaacc
     6961 aacaaatggt ttggacccat caggcgttat tgaaattaga gaattatgta aagagatgaa
     7021 aagacaagga aaaacattac ttatttctag tcatattctt tctgaagtgg aacaagtagc
     7081 agatgaagtg agtattattg atgagggaga gcttgttaaa tatataaatc taaaaaaaat
     7141 gaatgaaaat agccaagtta ctattcatat ttttacatct aattttgata aagtaaaatc
     7201 agtattatta gaagcaggag ttagccaaca taatatgata aatacagcaa aaggagtacg
     7261 attgctttct aaagatatgg atatatctga agtaaataaa ttactaaccc aaaatgatgt
     7321 cgatatcgag ggaattatga gggagaaatt gacattagaa gaacaatttc aacgtgtaac
     7381 agaaacgagg taatttaatg ttagatttag tatcgacgga gataacaaaa ataactcgaa
     7441 tgaaacaaat gtgggtatat gcaagcgtat tagggattta tattacgatg atgtcggtgt
     7501 acactgccca agcagttgga ttatttgatc aatttattca tttatacaaa ttttcattaa
     7561 gttatacagg atttctatta ttaccattta ttttattgtc tttgagtagt agcacaattt
     7621 cagatgatta tcgtaatgaa gttatgaaaa atttaacggt tattccaatt tccaaacaaa
     7681 aaataattgc cgctaaatta attgccattt atattgtttt gagtgttgct atggtaatga
     7741 tttggataag tgtttgtgta attgggtatg taacaaggtt tcgatttagt atgacatggc
     7801 tattgatttt ccggtatctg tggttatgta ttttaacttc acttgtgatt tttttatcaa
     7861 tgctacctgt tacactgata tccgtagcca caaagggaaa tgttgttata actaatttaa
     7921 ttggaagcat gtatattatt gcatcattct ttttaacaaa tttcatgaac gggattatcc
     7981 ctttggctac agctccgcat ataatttggt atggtagtat ggaaggtgtc gaagttaata
     8041 gcaatattgc actaatggtt attagtataa ttttctatac aatcatcgtt ctttttgcca
     8101 tgaataaaat tttgaagaag caagagttat gatctttcgg aggtattgta gatggtatta
     8161 gaatttaaaa aaatgaagcg gagtaagttg attattgttt taattttgat tgtagtgctc
     8221 aatataggaa ttcactatct aatgggtaat gtaaaatatg tgggcatccc ttatgctgct
     8281 gaacccggat ggactttaca gaatacattg attgtgggga cgtattatct tctactgcca
     8341 gtattcacat taattggatc agcgtctttt ataattgagc gagagaatag tgtttacata
     8401 aatattttaa caattcctgt tcgaaaaagt catttaattt tgaataaatc taaatttatt
     8461 tgggttgtaa gtatgatatt tacatcaagt atatttgtgt ttacattagt attagaaaaa
     8521 atacacagta atatattaac tcaggaaatt gtattaaagt atttatttga atatgtcgtg
     8581 cactctaatg gtctgtatgt gattgcaatg ttaattttgt cggtcataat ttacatggat
     8641 tataatatgc aactagctgt tatggtaggg tttgtaagtt cttttgtcag tgtttttata
     8701 gaacaaacgg caatatccta tttataccct gtaaatgcat tatttaatat ttcaggattt
     8761 aaggagtcaa atctatatga gtatagttca tcggttgtta ttttgtgcgt gatagcaatt
     8821 tgtgcagtgt tcatttataa aaaaatagca caacaagaat catggtaaat atagcataaa
     8881 tgcttattta taaattaaat gagaggagga aatattatga agaacaaagt gaacaactgg
     8941 aagacacctg tatatgagaa agaagaggac tacgataatc cagcaggaga tattttcaga
     9001 gaattgaaga gtgacgatat tgataaagta atggcatcag gtacagctaa tacttactgt
     9061 cgttgttatt caggaagaca ttcatgtggt cgtgcttgta cgattactgc tgaatgtcca
     9121 gtatttactg tagcgtgctg ttaataagat tgtgtgagga ggtgtactac aggtatttcc
     9181 tcctcgcgta catatagaga agaaaatact gttttcggga ggtaaaatga atgaaattga
     9241 tagataagtt taaaaatggt ttatattctt ttgaaagaga tattcagcat gatgagacta
     9301 ataattataa atcagaaaac aggttacagt attggaaaaa atttctaggt gttaacgaaa
     9361 gagaaattga gaatatttta tccaatggat tagggataaa tacagctaat ttaaacgaat
     9421 tattatcgga aaatgataat ttctcatgca aagttactga aacgaatgta ttgtggaacc
     9481 aattgattca cgacttacaa gtcctttcca ttgaatctat tattttacct gaattttata
     9541 taataggaga tattgggcaa aaagaactac caatgtttta tggttttcat gaaccatttt
     9601 taaaattagc gatattaaga tttgaaaact attggaaaaa tattcccggt atatcagata
     9661 atgtatttaa taaattatta atctatttat atgaccaatt agccgaaatt tcatatagaa
     9721 ctcttatttt agaattaaat atagctagag aagaaaacaa actagcagga gaaacatctg
     9781 aggaaagata taattatttt tcgactcaat acttaagtga taattattgg ttgattttag
     9841 aggaataccc tgtaatgttc aggttgatgt gtgaggcaac tcaaaaatgg ataaataata
     9901 caacacgatt tatagataga attttgtcag ataaagatga tttagaaaaa cttttaaaat
     9961 tgagggagaa ttaaacagta ttgaattgaa tacttctgat gtgcacaatg gaggacaaac
    10021 tgtaattata cttcattttg aggaaggtac ttctgttgtc tataaaccaa gagatttaaa
    10081 aatggatgta acttttcaaa gtacaattaa atggttcaat gaagtgacca aaagtcattt
    10141 atatagttta aaaatcataa atcatactga atacggttgg gtagagtata ttccacacga
    10201 agagtgtaag gattatagtg atttcaaaaa ctattatact gaattgggac aattattatt
    10261 tttgttttat ctacttcgtg ggaatgatat acattatgaa aatattattg caaaaggaaa
    10321 acatcctgta ttgatagact tggagacatt atttcataac aatacttcaa atacgagtgg
    10381 tatagataca gctgctgata gagtaaatga actattagag aattctgtca ggacagtagg
    10441 aattttacct aatttagttt gggcacaaaa tggtaaaaac ggtgtagata ttagtgccat
    10501 atcaactagt gagaataagg aaattcctat agaacaagcc agtattacta atgtaaacaa
    10561 agataatatg aaagtagaat ataagacatc tacgttagca tctcaaaaaa ataacccata
    10621 tataatagga gaagaaatta gtcttacaag ttatcataag tatttgaaaa aagggtttat
    10681 tgaatcatac actaaaatta aaaacataaa taaaaaagaa ataataaatc aagttgaaaa
    10741 ttacaaagag atttatgcaa gacaaatttt aagacctaca caatattata caacattaat
    10801 ccagatatca ttgcatcctg attttttgag aagtgctatt gatagggaaa tgttgttttc
    10861 caagctatgg atatactttg atgaaaataa ttcatttaga aaagtttcgg aaatagagtt
    10921 tacttctttg ttaaagaatg atattccgtg gttaatatca aatgtcagca aaaacaatat
    10981 aacgactaag gatggttcag aaattgaatc gatattcaag cactctagta ttgctctcgt
    11041 taaagaaaag ataaatattt taggggacaa agatttgacg ttacaggtgg aattgattga
    11101 aacagctttg aattatgatt ctgaatataa taaagcggag tcccagcgag aaaatgatag
    11161 aaaaattata gagataaatg atgacaaatt aaataaaaat catttagacc agcaactttt
    11221 agaaatatct actaatatag gtgattattt gattaatcag tcatttattg gaatgaacgg
    11281 ggatgttagt tggattgata tgaatgtaat tggagaaaaa gctaatgatt ggaacatggt
    11341 gcctactagt atggatttat acagtggatt atcaggaata atgatttatt ttatatttct
    11401 gtataaagaa actaaacaaa acaaatattt aatcatggta aaaagatgtt ataaaagtat
    11461 tataaattat ataaaaaatg ttagaaaaag aacaaatatt aattcagagg ttatgtttgg
    11521 tggtttttct ggtgaaacac ctattatata cgcacttacg atactagaag aagaattagg
    11581 aggaatattt gacttagatg aattagaaaa aataagatca tggattttca aagaatgtaa
    11641 gaaaaatata tcagttggaa atgagcatga tattataatt ggttcatcag gggtaatagc
    11701 tattttattg agatattacg atttgacatc taatgatgct attttagaag tgtgtcaaca
    11761 gtatgcagaa caaatcattg ataactatat agagatggat aacaacagca ttgcttggat
    11821 aggtattgct tcgagaaatg cattgggagg ttttgcgcat ggcgtttctg gaattgtttg
    11881 ggcattatcg aaactgtatt cttacttacc agatgaacga tatatagaag ttattgaaaa
    11941 agcactaaga tatgaagatt atttatatag tgaagatgat aaaaactggg ttgatagacg
    12001 tgaaacagaa gaaggtattg aatataataa cttgagttcc aacatgcccg ttgcatggtg
    12061 tcatggagca agtggaattc tattaagtag agcgtcatta aaaaaacata atctaccttt
    12121 atcagaaaaa agaaaaaata aaattgatga agacattgaa attgctgtta gaaccacttt
    12181 aaaaaatgga tttggacata gtcactgttt gtgtcatgga gatttgggaa atatgttaat
    12241 tctaaagtat gcatcatcag aattgaatac gaaaaatgat atagacaaaa gatacgatat
    12301 ttatatgtca catctgatta gccaattaaa agataagtgg gagtgcggaa ttccttataa
    12361 gaattctcca ggtatgatgt tggggctctc aggaataggg tttggattat taagtttaat
    12421 gaatgaagac ctaccgttta tattattact tgaataaaat tttaatatat atagctctat
    12481 tctactttag gtaactataa aaggtagggt tagaaagtag ggattatatt gaaaaaaata
    12541 aaatttgtac aacaactttc agaatctgac tgtggaattg ctgcactgac aatggtatta
    12601 aattactatg ggtgtaaact acatatatct gatttagttg aaaaatgttc tatttctcgt
    12661 gatggtgtcc acttaagaga tttaatgaat gtagcgaatg aatatggatt aaaatctaag
    12721 gctgttgaat taaataagaa agaaaatatt ttttctgaaa agattgtatt tccatgtatt
    12781 gctgtattat caatgagtca ttatgttgtt attgaaaaag ctaaaagagg aacaatttat
    12841 tatttagatc ctgaatgtgg cagaataata atggatggtt ctgaattttc gaaaaaattc
    12901 acgggtattt tactcctgtt ttttccagag aatataaaaa aacaaaaatc ccaaaataaa
    12961 ataattgaaa tattaacact tggagaaatt aaaaaaaaat ttatagttgg tgttatattt
    13021 ttttcgttta taatacaact atttgtattg ctaatgccct tatttactga atatatgata
    13081 gataatgtga taaatggaat tgcattagtt tcccatgtga aattaataat tgggatatta
    13141 tttgctattc tatcttacgg cgtattctct tttgtaagag aattattaac aacattatta
    13201 gaaataaaat atattagtac tctaaaaaaa atgtagtgcg taaattattt tatctaccac
    13261 tttctttttt tgatgttagg tcttcaggag atattgtttc tagaattaat aatattgatt
    13321 caattcaaca attgttatca aatataatta cgggtgtgtt tgtagatgtg ttaactatag
    13381 taatatctat atcaatgatg ttatatattt caagaatatt atcgattatt atgatagtat
    13441 acgggatact gttgtgtgtg ttgttaaata tattttttaa aaagacagat gtaaaaaata
    13501 aactatcatt aatgaaaagg gaaaagacac aaagttattt aattgaacta tcgtccaata
    13561 ttaatatgat gaaaacatca aattatggcg aagctctata taataaatgg gttaagaatt
    13621 ataatgaaca aatgcattta gaatttaaaa ggcagcgatt attagggatg tacaggagtt
    13681 taattatctc ctatagattg ttaccaagcg ttactgtatt atttataggg agtaactttg
    13741 ttaatcaggg gatgttaact ctcggtcaag ttatgtcttt tttagcttta ggaaatacat
    13801 tattgagtcc attagctatt ttaatacaaa atatctttga ttttcagtat agtaaaaata
    13861 acatagatag actttcagag attatcacag ctaaaagtga aaaaaatttt gatggaagag
    13921 aaataaatag ttttaaaaat attaaattcc aagatgttaa cttctcatac agtgggatca
    13981 atggaaaaaa aatgttaaag agttaaattt caccataaat gaaggagaat ttatttcttt
    14041 tgtaggaaga acaggttgtg ggaaaacaac tattataaaa ttgattttat ccttatataa
    14101 taattttgaa gggaatatat tattaaataa tatagataaa gaagtatatg atttgaaatc
    14161 ataccggcag ttattcggaa cagttttaca agatgagacg ttacttaacg atacaattca
    14221 aaaaaatatt gatccgacac atagtcatac aatctcaaaa ataagagaag cggcaaagct
    14281 tgcttgttta gatgaggatg tgatgaacag aatgccatct aaatataata cagaaattgg
    14341 agataatggg agaaatcttt ctggaggtca acgccaacgg gtggctattg ctagagcatt
    14401 attaactaac ccaaaggtca taattttaga tgaggggacc agtcaattag atgtatctac
    14461 agagagagaa attttcaata atttaaagaa aaaaaatatt actattatag cggtaactca
    14521 taaactttct accactacta tatctgactg tgtttatgta ttaaaaaaag gaaaaattat
    14581 agcacacggg aagcatgaaa aactaatgca gaataataga tattatagtg atttttttag
    14641 ataattatag atttaatgga tattattggt ccgtcatagc tgtcataatt tctgtccatt
    14701 ttttatatgg gcctctattc tgttgcggga gaaaaaatgt aggataagtt tctgaattag
    14761 tcactgtaat cagtgaaaag cttaaattct ggatatgtgc tatcttattt aaataaggaa
    14821 caattttata catgaaatac actgttgtgc gtgctataat ggacatatga gaactatttt
    14881 ataacggtat attgttcata tatttataaa gggaggacat atttatgtta tatgaagttg
    14941 ttgcagtgaa tgagacaggt attgatgggc agtcttactt agtggacggt gagtcgtatc
    15001 agacgtccag tcccacgagt gatgagccag ggactaatcc ggaacaattg atgggattat
    15061 ccttagcgac gtgctttaat gcgacgttgc atgccgtatt gaaagagcgt ggacttgagc
    15121 cagaaagtcg agtacaagta actgtacagt tgcatcagga tgaggagaat aaggaatatt
    15181 atttcacatt ggacttagaa gcagcggtgg ataagattga cttacaggaa gctaaagaga
    15241 ttgtcgaagt agcgcataaa cgatgtccgg tagcaaaaat tgttggcaat tatgaaggaa
    15301 tgacagtcaa aacagtacca tttaattaag ggtgacgtgc atttagaaag gggtgggtct
    15361 gatcagaaca ataaagaaat taggaaagct aatcagcagt ctcttggcta ttgttggtgt
    15421 catttggatt gtgggctatt attgggctga cgtagtgatt cgctttacta ggatattcag
    15481 caagttgcat cagcggttta agcgtcgtcg cattaagcca gctaagcaag cgaatgtgta
    15541 taaaaatatt ccttataaaa gtaactatcc gaataataag ttagacattt acgtaccttg
    15601 tgagtcaagt agcgttcatc ccactccaga gttaacagat caaaagcaag aaaaagatcc
    15661 gtctaatcca aatgttgtag aagattgcca gactgaaatg ttcgatgtat tgaaaccggt
    15721 cattgtctat ttccatggtg gtgggtttgc ttggggggat aagatcgatg agcggcgata
    15781 tttgttagag tttgtgaagg ctggttatgc ggttgtagcg gctaattatg cgctcacacc
    15841 gatgtataaa tatcccgtac cgattataca agctagcgaa gtgatggatt tcttagtgaa
    15901 cgaaggggaa caatatggct tagacccgga taatattgta ttagcaggat tgtcagcagg
    15961 tggtcattta gccgggcagc tagcgttagc tgaatcatcc agtgcttatg ctgatcgctt
    16021 agagctcagt caacatccgg acttaactat tcgcggcatc atgctcaaca gtgctttaat
    16081 tgatcccaat cgttccaatc aggtgggcca ttggttaatt aactggctat tcagtgtgat
    16141 gggattctca tatatggata tgaatccacg caatatgaaa gagcagatgc tggctgaagc
    16201 gaatcttctt aatcatatca acgaagccct tccacccgtc tatatttcgg atgggaatcg
    16261 ggtctctttt acgcaacaag ccactgatct ggttgctcaa ctgcgagcac gtgatattta
    16321 tgtccagtct aatatttata ctgatgacta tcccggcttc atgttccacg gctacgaagg
    16381 ccagttgtgg ttaagagaag ctcgcgaaaa tgtcgataag cagctggact tcttggagac
    16441 ggttattgat tagcatagcg aataacattg aaggttgatt ataaactaaa ttatattgaa
    16501 agataagctc cctccgaaag tagatgtctg tattgtctac tttggaggga gcttattaag
    16561 aaatttttat tttaaataac tgaagtgaca tcatttacat tagaaaaatc gcttttatct
    16621 tttgtagtaa tgttggattc ttacacccat acactttctc ttttccgtaa agtacagatt
    16681 ttactaaaaa aatgatatag gcggaaaaaa tatatactag cacaagcgtt agaaatatat
    16741 ctacaatttg ataattcata ttattcccaa catttccaaa cagcagcagc tatgctacca
    16801 ccaccgacca ctactattcc acctaaacat acaatacatg taggaggtgc taacacacaa
    16861 ggagttccac aaactttagc tattaatcct gaaacagttg cccccgcacc agatacagct
    16921 aaaaagcaag atacatttaa acctctttgt tgagattgta ttgcataatc atttaattct
    16981 cgtatacctt gaagtatctc atcattgtta acgaattgaa tttctgaatc cacatcactt
    17041 ataacttccc catcttgctt agtttttatc caaaagttgc catctttttc atagaagtaa
    17101 ttttcaacat aaaaattttc atttgtattt agtggtattg taacggtgct taaaacgcta
    17161 tcatttttag aaatagttgc atatacttca ccattcattt cagctatttt tatattgtca
    17221 aaatcaaaat actcttcaga atattcaatt tttccgtttt gaaataaact attaaactta
    17281 tttacagcat ctgttctcaa ctcttccgat tgaatgattc ttaaatcttc atattttttg
    17341 agagaggtag cttcgtattg attttcactc gcaataactc tattcggggt tataactgat
    17401 atcatcagtc ctaatgtcaa tataaaagtt gctacttttt tgatcttatt taacatagta
    17461 ttccctcccg ttaatagttt agttttattc tcacaaatat aattacacca acttaactat
    17521 tgcgcctttt ttgcatatct atataatcat tgtcgatgca cctcctttaa ctttacagtg
    17581 attgattatc acaataacaa taattttctt ctccatctaa atcgaatacc ttatagatgg
    17641 aggtgtttaa tgcatcagca atatcgctag caagctttaa atttggtatt gtttttcctg
    17701 tttctatttt atttatgctt actcttgata cgccaacttt cttacctaat ctatgttggg
    17761 ataaccccaa cggctttctg aactctttta tcctattctt cattaattcc ttttcactct
    17821 cctaaagtta actttagttt accacacttt tatatttata gcaacatatt tttaaatttt
    17881 aacaaaaaat tttactggtg ttgcataaac ctgaaaacaa ctagttagtg aattcgcgaa
    17941 taaatggtat tcattaataa caataatatt gtgcggtcat acagctaata acatgttaat
    18001 tatgaataaa cactataaat ggggtgttag gtttactata ttataaaact aaaagaattt
    18061 tagattataa gtgattgtgg ttgaattcct tgctggtata ataaccgtaa gatatatgtt
    18121 acaatgaaaa agatgagagt aggaaaattt tgagtcatta gacagggagg aaagggtgtt
    18181 gtagatggat aaatcttaca tattcgcagt tagtgatgta catggtagtg atgagcaact
    18241 tgaggcatta cttgaatatt ggaatccaga agaagagcag cttgttattt tgggagattt
    18301 gtgtgatcgc gggccggatg ctcaggctgt ttttcgccga gcgaagcagc tgaaggaaga
    18361 atacggagca atctgtctgc gtggaaatca tgaagatatg ttgatgaagt tcttggatga
    18421 tccggctgat aatgtggcgc actattatcg gaatggaggc gagataacac ttgagtcgtt
    18481 cttaggtgat gcggtatcgt caagtacccc tcaggaattg gccgaagcat tgaaagatca
    18541 gtatccatgg ttgattgact atctggataa tcttgagtta acttatgaat gggggaagta
    18601 tttctttgtt catgcgggga tggacttgac gttagatgac tggcgtgagt caacggttcg
    18661 agataaaata tggattcgta aaggattctt ggatgtgccg aatgagacag agaagacatt
    18721 tgtgttcggt catacgccga caatgaagtt gcgcacggat ggacaacctg atctttgggt
    18781 gagtgaagat cataagattg ggatcgatgg cggagctgtg tacgggggtc agctaaatgg
    18841 tgtgaagttg agtcaagctg ggattgaagc gacttaccga gcataagata aacagaagta
    18901 gagaatagag aaagatggag tgatcagtgt ggaagataag cagacgtttc aactaattgt
    18961 acgggagtta agtgattatt cgactaaaca agtgcggaca gtattgtcgc tcttggacga
    19021 tgggaatacg gtgccgttca ttgcacgtta ccggaaggat cagacggggg cgctcgatga
    19081 agtccaaatt agagagatcc aacagcgcag tgaatatatc caaaacttac aagaccgtaa
    19141 agagaccgtc ttaagtgcta ttgaggagca gggggaactg acggatgaac tagcagcgga
    19201 aatccgagag gcaacgcaac tacaagaagt agaagacttg tatcgaccgt ataagaagaa
    19261 gcgtcagacc ttggctacta aggcgaaaga agcaggatta gagccattag cagactggtt
    19321 gcttggtttc cctgatgcaa gtgaggcaga gattgaagcg atagcaagtg attatcttaa
    19381 ttcggaatat gatgttgaga cggtagcgga tgcgttggcg ggagctcatg aaattattgc
    19441 tgagcaggtt gctgataatc cagaatatcg caagcgtctg cgcgactata caatctataa
    19501 cgctcagata actagtacgg tgaagaatgg ggagattgat gagaagaatg tcttccaaca
    19561 atactatgag tatgcagagg attaccggaa aatggcacct caccgaacac tcgcattgaa
    19621 ccggggagag agtgaagagg ttttaaccgt taagttagag gtcggtgatg agcgagcgca
    19681 tcggtattta tacaaacaaa tattacctag tctggatagt tcatcgccag taatctctat
    19741 cgtgaaggaa gcaatcaaag atgcgtataa gcgatttatc gcaccatcca tcgaacgtga
    19801 attgcgtggt acattgacgg agaaagcaga gactcatgcg attcagattt tcggtgagaa
    19861 cttgaaaaac ttactgatgc aagcaccact gaagcagaaa acaatcttag ggcttgatcc
    19921 agcttatcgg tcagggtgta agctagcagt gattgattcg acaggaaaag tgttagctat
    19981 taaagtgatt tatccgcata catctggtga aacaaaacgt aatcagtcga aagaagcact
    20041 gaagcaattg atcacggaac atgcagtaga tgtgattgct attgggaatg gtacggcgag
    20101 tcgtgagtca gagcagttcg tggctgaagt ggtgagtgag atgccggaga agacagcctt
    20161 tattattgtg aatgaggcgg gtgcttcagt gtactcagcc agtgatgaag cgcgacgtga
    20221 gtttcctgat ctagcagttg aggaacgttc agcgattagt attgctcgtc gtctgcaaga
    20281 tccattagca gaattggtca agattgatcc caaatcaatt ggtgtcggcc aatatcaaca
    20341 tgatgtatca caaaaagaat tagaaggtca gctagatttt gttgtcgaga cggcggttaa
    20401 ccaagttggt gttaacttaa atacagcaag tggtgcgcta ctcgaacatg tctctggttt
    20461 gacgaagaca gcggctaata atattgtcgc ctaccgtgag tcagagggtc agttcactac
    20521 gcgtgatcaa attaaacaag tgaaacgctt gggaccaaaa tcttatcagc aagcagtcgg
    20581 ttttttacgt attccggatg cacctgagcc gttcgaccaa acgggtatcc accctgagtc
    20641 ttatacggaa gctgaaaaaa tattagccca tgtgaagcga gataagtcag ctatcgggtc
    20701 tgatgagttg aagcacttgc tggaacaatt gtcagacgtg cagacagctg aacaattaga
    20761 gattggttta gagacatatc gtgatattgt ggcagctttg attgcgccgg gacgagatgc
    20821 acgtgatgat atgccggccc cgattctatc gacagatgta ctgaagatgg atgacttacg
    20881 cgaagggatg caactgcaag ggacggttcg taatgtggtt gattttggtg catttgtgga
    20941 tattggagtg aaggaagatg gcttagtcca tatttctgag ctaagtgatc aattcgtcga
    21001 acatccgaaa gatgtcgttc aagtgggcga tattgtgaca gtttggatta aatcgattga
    21061 tttggaccgt agccggattg gtttaacgat gatacagaaa gattagtaac gagagaagga
    21121 agacagagta atgaaactca tacggacaaa cgattccttg caagcttatg ttgaagcagt
    21181 atctgaagaa tggtttcacc atcctttcag acatcgagca acctttaatt cccgcttgcg
    21241 aacaacgggt ggacgttacc acctccagac acatcatttg gacttcaatc ccaagatttt
    21301 agacgtgttt ggagaagccg tctttttagg tattgtgaag catgaacttt gtcactatca
    21361 tctgcacata gcgggtcggg gctatcaaca tcgcgatgct gattttcgga aattgttgaa
    21421 agaagttgga ggcttgcggt ataccccatc gattgaacag caaaaaggtg tggcgaaacg
    21481 ctggcattat cagtgtagtc gctgtgggca gtcttattat cgtaagcgtc gtgtgaatac
    21541 atcatcatac tgttgtggtc gctgccgtgg gaaattaaaa ttattcgaac aagtgatatg
    21601 tgcacgatag ataattagtg acatgatgta agtgatgcat actatcttta tcattataat
    21661 gaaagcagtt caattataga aaagggttac tgtaaatggg attattattg aaaaatatta
    21721 agttagagcg ttgttataag gaacaaaata atggcttagt tgtgacagag acggaaacaa
    21781 aagatctatt aataaaggaa ggctgttttg ctgaaattgc ggatgaaatt gctgaaaata
    21841 cgccaggtgt gcaaaaagtt attgatggga acgggcaatt gcttgtgcca tcattacggg
    21901 agtcgcatat tcatatcgat aaaacgtact ttagcgggcc atggcaagca cctacccgac
    21961 cagaagattt cagtatttat acacggttga atgaagaaaa agagttgcta ccagctcagt
    22021 tagatgtcgc tgccgaacga gcacataaag tggttcaaca ctatattcaa aatggtcata
    22081 ctcatattcg aacgcattgt aatattgatc cccaaatcgg tctaaaacat gtcgaattaa
    22141 ctcttgatgt actcaaacaa tatgaagata aaataacgta tgagattgtt gctttcccac
    22201 agcatggttt actcagaaac ggggaaggat tcatccaatt attcgaacaa gcactgagta
    22261 tgggggtaac gcatattggc ggcttagatt ttgctacatt agaacgagat gtagaggcta
    22321 gcctccaatt gctcgttaag tgggcaaaac aatatgattt agggattgat attcatttac
    22381 acgatcgtgg ggcattaggt gtctatgaga ttgagcgttt attggatatg atggcaatgt
    22441 atgattatca agggaag
//
