LOCUS       NZ_NAQX01000009        12154 bp    DNA     linear   CON 09-SEP-2024
DEFINITION  Dolosigranulum pigrum strain KPL1922_CDC39-95
            NODE_10_length_97139_cov_86.161758, whole genome shotgun sequence.
ACCESSION   NZ_NAQX01000009
VERSION     NZ_NAQX01000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Dolosigranulum pigrum
  ORGANISM  Dolosigranulum pigrum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
            Dolosigranulum.
REFERENCE   1  (bases 1 to 97277)
  AUTHORS   Brugger,S.D., Pettigrew,M., Kong,Y. and Lemon,K.P.
  TITLE     wgs assembly of Dolosigranulum pigrum KPL CDC strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 97277)
  AUTHORS   Brugger,S.D., Pettigrew,M., Kong,Y. and Lemon,K.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2017) Microbiology, Forsyth Institute, 245 First
            Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. DECEMBER-2015
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003264145.1-RS_2024_09_09
            Annotation Date                   :: 09/09/2024 01:06:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,809
            CDSs (total)                      :: 1,722
            Genes (coding)                    :: 1,695
            CDSs (with protein)               :: 1,695
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 11, 17 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 11, 17 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 4 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:30:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 83001
            Orig. end                         :: 95155
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NAQX01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12154
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12154
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12154
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:7154](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7154
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(808..1695)
                     /locus_tag="B8A46_RS09400"
                     /pseudo=""
     CDS             complement(808..1695)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016120.5"
                     /locus_tag="B8A46_RS09400"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="homoserine O-succinyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MIMPIVCGQNRYGSDVSMISNAEIQMNQTHPIKIAILNLMPNKIT
                     TEHQLLRLLGKSPLPIEVTLLYTKTYAPTHMMDDYLQKQYRVFDDVKYDTFDAFIITGA
                     PVEHLSFEQIDYWDELTDIFTYVHSNVRSTLFLCWASQAALHFYYDIPRFEVSEKIFGI
                     ERFKTRVKNSLTDGFESYFNVP"
     gene            complement(1724..2194)
                     /locus_tag="B8A46_RS05195"
                     /old_locus_tag="B8A46_05150"
     CDS             complement(1724..2194)
                     /EC_number="4.4.1.21"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648167.1"
                     /locus_tag="B8A46_RS05195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_05150"
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="WP_112789266.1"
                     /transl_table=11
                     /translation="MEKIASFTVDHLNLKPGLYLSRTDQIGDRKLYSYDLRFTAPNYEP
                     VMNTAEIHTIEHIVATYLRNDEQFKQEIIYFGPMGCRTGFYLITTDAIAPADLLDLLIR
                     AFQFTANFQGEIPGADARSCGNYLDQNLPMAKYYAKHYVTVLENVTDLTFSY"
     gene            complement(2714..3469)
                     /locus_tag="B8A46_RS05200"
                     /old_locus_tag="B8A46_05155"
     CDS             complement(2714..3469)
                     /EC_number="1.1.1.100"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (4-126). E-value: 5.7e-08. Score:
                     24.8. Matches aSDomain:
                     nrpspksdomains_B8A46_RS05200_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF006393.0"
                     /locus_tag="B8A46_RS05200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_05155"
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="WP_111951094.1"
                     /sec_met_domain="adh_short_C2 (E-value: 2.1e-38, bitscore:
                     125.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKVVIVTGSSRGLGKAIAQKFADQGAYIVINYVKSEDKAQQLV
                     QDIGEDRAIAIQADVRDSEQINHLVTKTIEHFGQLDVIVSNALINFKFDPHAQQSLDTI
                     DWKNYNEQFEGSVKAALNLVQASLPYLKDSPQARIITIGTNLFQNPVVPYHEYTSGKAA
                     LLGFTRNIAKELGPDGITANMISGGLLQATDASSVTTPEVFDLIANSSALQRVTTPEDV
                     AEVVAFIGSEASRGLTGQNITVDSGFTMN"
     aSDomain        complement(3092..3457)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B8A46_RS05200_PKS_KR.1"
                     /evalue="5.70E-08"
                     /label="B8A46_RS05200_PKS_KR.1"
                     /locus_tag="B8A46_RS05200"
                     /protein_end="126"
                     /protein_start="4"
                     /score="24.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VVIVTGSSRGLGKAIAQKFADQGAYIVINYVKSEDKAQQLVQDIG
                     EDRAIAIQADVRDSEQINHLVTKTIEHFGQLDVIVSNALINFKFDPHAQQSLDTIDWKN
                     YNEQFEGSVKAALNLVQA"
     CDS_motif       complement(3386..3454)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B8A46_RS05200_0001"
                     /evalue="1.30E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="B8A46_RS05200"
                     /protein_end="28"
                     /protein_start="5"
                     /score="14.7"
                     /tool="antismash"
                     /translation="VIVTGSSRGLGKAIAQKFADQGA"
     gene            complement(3558..4127)
                     /locus_tag="B8A46_RS05205"
                     /old_locus_tag="B8A46_05160"
     CDS             complement(3558..4127)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Mac"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1156: Chloramphenicol acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635128.1"
                     /locus_tag="B8A46_RS05205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_05160"
                     /product="sugar O-acetyltransferase"
                     /protein_id="WP_112789267.1"
                     /sec_met_domain="Mac (E-value: 7e-13, bitscore: 41.4,
                     seeds: 568, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKHEYNKMIRGELYDPRDPYLRDLMTKQHALMDAFNESSRTDHA
                     TQNKLLEKLLGQYHGESSHIRRPFFIDYGLNITIGKHFFANYNCTMLDVAPITIGKNVM
                     FGPNVSLNTPEHPLSAAERNMGLEFARPITIEDSVWIGASVTVVGGVTIGQGAVIAAGA
                     VVTKDVPANTVVGGIPARVLKTIENC"
     gene            4348..4701
                     /locus_tag="B8A46_RS05210"
                     /old_locus_tag="B8A46_05165"
     CDS             4348..4701
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B8A46_RS05210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B8A46_05165"
                     /product="hypothetical protein"
                     /protein_id="WP_112789268.1"
                     /transl_table=11
                     /translation="MHTRFKEGLKSFIISYVAMILYHLPSAYNPLFDIVHMMVIVGTLL
                     YGGYYFYRNGASSNGMAGLLIQVSLFTIGFNIALPLTREIFNARIMFMTGCSLSLIFLK
                     HIENNLYRIDRKS"
     gene            5001..7154
                     /locus_tag="B8A46_RS05215"
                     /old_locus_tag="B8A46_05170"
     CDS             5001..7154
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /locus_tag="B8A46_RS05215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_05170"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_112789269.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.6e-38, bitscore:
                     124.2, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 5.5e-235, bitscore:
                     774.6, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTNFRVIYQQDNMDCGTACVQMILHHYNSEFSIHKLKDLTDKGK
                     QGTSALGIKRCLESLNFDCLVIDADNAVWTDSAMTYPAIAHLNVHNGKHYVVVYGYQDN
                     KLFIADPDDGKYNMSIETFSAVWSRTLFVPSPGRHYKPIKEKVQGLMSFVPKIIQYKKF
                     VVFSIIASLLIMGLSIGSSYYFQGIIDSIIPANNISMLNIISFGLMISYVLRVIFGYIR
                     NYLLFILGQKLSRDIILNYFKHVLKLPISFFASRQSGEIISRFLDASTIIDALANTSLT
                     LLLDSIMVVVVGIVLLLQHNVLFGVTLVTLPLYIGTIILFIIKLDKSNEEEMESAAVLN
                     SSIIESLNGIETIKSYNSEKVIYQRVVGEFEDLMIKTLTTMTLDNMQGAIKTGTQLVGN
                     ALVLWLGAYYVMSGEMSIGALITYNALLVFFTEPLESVINLQVQIQKAQVASRRMNDIL
                     SIEVEGNISNDTVEIEEMGEADLKFDDVYFSYGLGELILRGITCEIKVGDKIALVGESG
                     SGKSTLAKMLVRFYDPSMGNIYFGKYDINTIDVHILRHNIVYVPQDSFFLAGTIRDNLL
                     FGLDYEPTQNQIEQACEIACIKSFIESLPLQYHTIVEEAGSNLSKGQKQRLAIVRALLT
                     NSQVIIFDEATSGIDVFSEQRIMDNIIKMTERTIIFITHSLPAAKLCEKIMVMDQGDLV
                     EEGIHEVLRYAGGTYQKLWEVIS"
     gene            complement(7219..7974)
                     /locus_tag="B8A46_RS05220"
                     /old_locus_tag="B8A46_05175"
     CDS             complement(7219..7974)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.5"
                     /locus_tag="B8A46_RS05220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_05175"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_112789270.1"
                     /transl_table=11
                     /translation="MYPLIICEDSIGQLNAIKQIINLYTGFYESRFNVSLITSSPHEVL
                     DYLETYQPKKGVFLLDVHLNSTIDGLDLAEAIRKNDVNAKIVFITTDEHAAPLTFKRNL
                     EAMSFIEKPSNVEELRKNIFETLNIAYNRLTTSLDEKKQLFTFSRYGETVNIDFEKVLY
                     LQTSIEESHKLTLTTTLGEYEFSGSLIKCEENFPELFRISKSALINPGNVVSINYKNRD
                     IVLKDNHIIQYAFRRAKSIKEKFSNPNKN"
     gene            complement(7992..9149)
                     /locus_tag="B8A46_RS05225"
                     /old_locus_tag="B8A46_05180"
     CDS             complement(7992..9149)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.5"
                     /locus_tag="B8A46_RS05225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_05180"
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_181453622.1"
                     /transl_table=11
                     /translation="MIIHDLFYATDIVALLYGLVLYGIFFSLKRINFEVLNSILLLVYT
                     KGIVEFLVALPINTLKYYNLHNHTLVMITTKILTFIVSIAIYKKIYPYFTSNIDQVKAK
                     KIVTYSLLILNILFSSYFIFIQWFDLYRKLLYFTLTITIIFVIITVVFLYALHKYLQLK
                     FQHELSNQHLEALTKYNHIVEEETYKTKEFQHDYKNLMLSTTSFIENNDLAGLKEMMYS
                     LIDYSKDYFSHSTFQYKDIHNIKHPALKSIILAKLVTFTELGITCQFECRKPLDNIYID
                     TFDFIRVVGILLDNAQEYVTTIEEKHIDILITSHTKVMELVISNPYIPDHYTLRNFKQE
                     GFSLKGNDRGMGLSTITKIEKKHDNLTVLFEKNTIFSAKIITTKL"
     gene            10107..10688
                     /locus_tag="B8A46_RS05230"
                     /old_locus_tag="B8A46_05185"
     CDS             10107..10688
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014566.5"
                     /locus_tag="B8A46_RS05230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_05185"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_111974131.1"
                     /sec_met_domain="Abi (E-value: 7.1e-12, bitscore: 38.4,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRYTNKVFMFHEIIICIACVFVAGLLVNIGNVLTIILPFGDKELL
                     NVLILSITSMAGFILFPYNLYKTMYSELNQPKISYKRAIILFIGAIITGLFTVSIFEII
                     QPFLIAAGEEFLFRFIIYKLLQNKFSKFQSMIVGSIIFSIVLHLNGNLLFNLVTKFPIS
                     MLLYYVYEKYGLQDCIAIHWLHNMLIGKFV"
     gene            10701..11120
                     /locus_tag="B8A46_RS05235"
                     /old_locus_tag="B8A46_05190"
     CDS             10701..11120
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF038270.1"
                     /locus_tag="B8A46_RS05235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_05190"
                     /product="Msa family membrane protein"
                     /protein_id="WP_112789272.1"
                     /transl_table=11
                     /translation="MYRKILLSLISTLIIFGTGIYLNVSMSLLMFAVISYILPLMGNIV
                     IDYYVQTENVLIGSGIISTITTTGYAIFAILMENNINFAGFIRQHTYRSGNMTMGLEPG
                     LADISQLIFIFALNLSVLYFIQYLSRGDFSHVRRK"
     gene            11104..11991
                     /locus_tag="B8A46_RS05240"
                     /old_locus_tag="B8A46_05195"
     CDS             11104..11991
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635115.1"
                     /locus_tag="B8A46_RS05240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B8A46_05195"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_112789273.1"
                     /transl_table=11
                     /translation="MLDVSNVSKQYSGNDFYSLKDVSFTIEKGEIVGLIGKNGAGKTTL
                     LKLLAKSHFPTAGVIRYQGLDIFSKDNVLRPFGLMIETVYYSHLTVKQNIEFYLDIHDQ
                     SEYKKNIQSVLETVELWHKKDFKPDGFSFGMKQRLSLAMSLVTNPEFMILDEPFVGLDP
                     DGVNDLMRILKQWAADRGTSIIVSSHQLNELEAICDRYLYIEGGALKKQFGKAAVEVTV
                     IELVDALQDRDALFSAYEAIQAISEDGKQIEVDSNSEAFHQILGTITASHNIKKIFTKE
                     NALNQYFKGSRDHE"
ORIGIN
        1 agtggataat acgttcccat caccatattt gttgaatccg attaagtttg gagcggatat
       61 tgttattcat tcggcgacta agtttattgg tggtcatggg acctcaattg gtggtgttat
      121 tgttgagagc ggtcagttcg actggacaca aaacaataaa ttccctctct tatcagagcc
      181 cgatgattca taccacggct tgatttttgc tgaagcagta cctgatgcag ccttcacgac
      241 taaaatccga gcgggccttc tgcgtgatac gggggcagct atttctccgt tcaatgcatt
      301 cttattattg caaggcgtgg agtcactaca tgttcgagta gaacgtcatg tggagaatgc
      361 cgaagcagta gcgcaattct tagctaacca cgagaaggta gaatgggtgg attatgcggg
      421 attagcggat agcccttatt acgaattgaa gcaaaaatac ttgccaaaag gagctggctc
      481 ggtcttcacg ttcggagtga agggtggcta cgataaggcg gttgaattta tcgaagcatt
      541 agaactcttc tcactactcg ccaatgttgg tgatgcgaag tcattagttg ttcatccggc
      601 tagtatgacc cacagccaat taacagagga agaattaaaa tcaggaggca tctatccaga
      661 aacaattcgc gtatcaattg gtatcgagaa tgtggatgat attattgatg atctaacgca
      721 agcacttgcg aaaatttaaa gtaaataatt taaaaagaca tccagcatat gtagcggatg
      781 tctttttaga ttcatcgttt tataagatta agcatctgaa acggttgggt caacaatatg
      841 cttgatccag ttagtgaaga agcgttctgc atgtgtctgc caagtggaac agtatccttg
      901 tgtcggatca tcattaacga aataacaggt aggcaatgat gtgtgtaaac cacgctcctg
      961 atcgcgctta tattcaaggt gcaacgtatc agtatcatat tcaaaatgcc cagcaataaa
     1021 gacagatcgg tagtccttgc tggcaacaat ttgggcacct gattgctcac tgccggcgta
     1081 taaaatcaaa tccgactgac tctgaatatc acttaattga tgataaaaat aacgggatta
     1141 aggcacatta aaataggact caaatccatc tgtcaagcta tttttcacac gtgtcttgaa
     1201 tcgttcaatc ccaaaaatct tctccgatac ttcaaagcga ggaatatcat aatagaaatg
     1261 taaggcggct tgactagccc agcataaaaa gagcgtactg cggacattcg agtgaacata
     1321 tgtgaagata tcagttaatt catcccagta gtcgatttgc tcaaatgata aatgttcaac
     1381 cggtgcacct gtaatgatga atgcatcgaa tgtgtcatat ttcacatcat cgaagacgcg
     1441 atattgcttc tgtaagtaat catccatcat atgcgttggg gcgtatgtct tcgtatagag
     1501 cagagtaacc tctattggaa gaggggattt tcctagtaat cgcagtaatt ggtgttcagt
     1561 agtaatttta tttggcatta agtttaagat agcaatctta ataggatgag tttggttcat
     1621 ttgtatttct gcattagata tcatagacac gtcagatcca taacgattct ggccacaaac
     1681 aataggcata atcatccctc catttccata tattactact atactagtaa ctaaacgtca
     1741 gatcagtgac attttctaag acagtgacgt aatgtttggc ataatattta gccatcggga
     1801 gattctggtc gagataattc ccacagctac gtgcatcggc tccaggaatt tcaccttgga
     1861 agttggcggt gaattggaat gcacggatta ataaatcgag taaatcagcg ggggcaatcg
     1921 catcggtcgt aattaagtaa aaacctgtgc gacatcccat tggcccgaag tagataatct
     1981 cttgtttgaa ctgctcgtca tttcgcagat aagtagctac aatgtgttcg atcgtatgaa
     2041 tttcagctgt attcatgacc ggttcataat ttggtgcagt gaagcgcaaa tcatacgaat
     2101 ataacttccg gtcaccaatc tgatctgttc gtgataagta caatcctggt tttaagttca
     2161 agtgatccac tgtaaagctt gcaatttttt ccattatttg tctctccttt atatgtttgc
     2221 ctacaaaaaa atacccgttc ctttgccgac taaatgtgta gtcaacaaag ggacgagtat
     2281 tgttcgtggt accaccctta ttcgcctgag tctttcaacg cagcgcctca tcagcacata
     2341 cttcctcgca gaaatataag cctagccata taacggtcgc acccgtcgag ccagcgtgaa
     2401 ccagctcaaa tcctcggagc tcatcttcat tcgcttaacc attttccctt cgcaccaaac
     2461 gggaatctct gtatacagcg ccacaaacta ctcttctcct gaaatgataa tcttttaata
     2521 ttaattaata gtttacagat agttaaaggt aatgtcaagt aggaaatttt atttttttaa
     2581 caagttagtt gatattgaga ggactatata aggcttctta ggagacatta cccataagaa
     2641 gccctatatt ttggtaattt aggttaaata agtacatatt tttccacaat taatcaacat
     2701 tttgtgtgct taatcaattc attgtaaaac cactatcaac agtaatattc tggccagtta
     2761 atcctctaga agcttctgat ccaataaatg caacgacttc tgctacatct tcgggagtag
     2821 ttacacgttg taaggcagaa gaattagcaa ttagatcgaa gacttctggg gttgttacag
     2881 aactggcatc tgttgcctgt aagagtccac ctgatatcat attagcggta ataccatcag
     2941 gacccaattc ttttgcaata ttacgggtga agcctaataa tgctgctttg ccagaggtat
     3001 actcgtgata aggtactact ggattttgga ataagtttgt tccaattgta ataattcgag
     3061 cttgtggact atcttttaag taagggagac tagcttgtac tagattaaga gcggctttaa
     3121 cggatccttc gaattgctca ttataattct tccaatctat tgtatcgagt gattgttgag
     3181 catgagggtc aaacttaaaa ttaataagcg cattactgac tataacatca agttgaccga
     3241 aatgttcgat tgttttagtg actaggtggt taatttgctc agagtctcta acatcagctt
     3301 gaatagcaat ggctcgatct tcacctatat cttgaactaa ttgctgggct ttatcttcac
     3361 ttttgacata attaatgact atataggcac cttgatcagc aaatttttga gcgattgctt
     3421 ttcctaatcc ccgactagaa cctgtaacaa tgactacttt tttctccatg atgattcctc
     3481 cttaaaatta tttcttataa agtatacgct aacatgtttg ttttttaaat tattagccct
     3541 tgaaataatg agtattttta gcaattctca atggttttca gtacacgtgc gggaatgcca
     3601 ccgacaacgg tattggcagg gacatcttta gtaacaacgg cacccgcggc aatgactgct
     3661 ccttgtccga tagtgacacc accgacaaca gtgacgctag cgccaatcca gacactatct
     3721 tcaatagtga tcggtcgggc aaattctaat cccatattac gctcagctgc cgagagagga
     3781 tgttccggag tgttcaagct aacgttgggc ccgaacatga cgtttttacc gattgtaatc
     3841 ggggcgacat ccagcattgt acaattataa ttagcaaaga aatgcttacc aatcgtaata
     3901 ttgaggccat aatcgataaa aaatggccga cgaatatgac tgctttcgcc atgatattgg
     3961 cccagtaatt tctctagcaa cttattttga gtagcatgat cggtacggct cgattcgttg
     4021 aaggcatcca ttaaggcatg ttgttttgtc atcaagtcgc gcaagtacgg atcacgagga
     4081 tcatatagtt cgccacggat catcttatta tattcatgtt tattcatcaa atcgctcctt
     4141 acgtctgttc attataccgg tcagaatata ccttggccaa tattaactat taagcgaatt
     4201 tatcgcagta taaacaagaa aaaatagcgt attcagacga attagagagc aggatggata
     4261 aaattatgat gctagctaaa taccggacgt atcctattta tgatatactt taggtaactg
     4321 tagcaataaa ttctggaggg gagtctaatg catacgcgat ttaaagaagg attaaagtca
     4381 ttcattattt catatgtagc aatgatactc tatcatcttc catctgctta taatccattg
     4441 ttcgatatag tgcacatgat ggtgattgta gggacgttgc tttatggagg ttattatttt
     4501 tatcgtaacg gtgcttcaag taatggcatg gccggcctgc ttattcaagt gagtctattt
     4561 acaatagggt tcaatatcgc cttgccactt actcgagaga tctttaatgc ccgcatcatg
     4621 tttatgactg gatgttcatt atcacttatc tttttaaaac atatagaaaa taatttatat
     4681 cgaattgacc ggaagagtta gtataatata taaaatatga aagaagctct agagcaatgc
     4741 agatatatgt tactgataat atccaaaatt ttattgggac ccttaaaatt gactttaaat
     4801 tctatatttc tacacagtaa tgatttgtga gtgtttaata tgtcttacgg gaatttttta
     4861 tttagttatt tgataagttg gtataatttc aaaagataac gataaggtag taaatttgta
     4921 cgtttgggga agaatactca aataaataaa ttagatgtca tactatagtt agtcagtgaa
     4981 gaaaatagga gacatatgaa atgaatacca actttagagt aatttatcaa caagataata
     5041 tggactgtgg gacagcctgt gtccagatga tcttgcatca ctataattct gaattctcta
     5101 ttcataaact aaaagatcta acagataaag ggaaacaagg cacttcggca ttaggtataa
     5161 agcggtgttt agaatcatta aattttgatt gtcttgttat tgatgcagat aatgcagtat
     5221 ggacagattc agcaatgaca tatccagcta ttgctcattt gaatgtacac aatgggaagc
     5281 attatgttgt tgtgtatggt tatcaagata ataagttatt tattgcagac ccagatgatg
     5341 ggaaatataa tatgtctata gaaacatttt ctgcagtatg gtcaagaaca ctattcgtac
     5401 cttcaccagg aaggcactat aaacctatca aagaaaaagt tcaaggattg atgtcatttg
     5461 ttcctaaaat aattcagtat aagaaatttg ttgttttttc gattattgca tcattgctta
     5521 taatgggatt aagtataggt agttcttatt attttcaagg aataattgat tcaattattc
     5581 cagctaataa tatttcaatg ttaaatatta tttcatttgg attgatgatt agctatgtgt
     5641 taagagtgat atttggatat attagaaatt atttgttgtt tattttagga caaaaattga
     5701 gtagggatat catattaaat tattttaaac acgtattaaa attacccata agtttttttg
     5761 cgtctagaca atctggggaa attatctcta gattcttaga tgctagtaca atcattgatg
     5821 cattagccaa tacatcctta acattacttt tggacagcat aatggtcgtt gtggttggca
     5881 tcgttctatt gcttcaacat aatgtattat ttggtgtaac tttagtaaca ttacctctgt
     5941 acattgggac aattatactg tttatcataa agttagataa gtcaaatgag gaagaaatgg
     6001 aatcagcggc agtactaaac tccagtatta ttgaaagttt aaatggtatt gaaacaataa
     6061 aatcatacaa tagtgagaaa gttatttatc aaagagttgt tggggaattc gaagatttaa
     6121 tgataaaaac attaacaaca atgacattag ataatatgca gggcgccatt aaaaccggta
     6181 ctcaactggt ggggaatgcg ttggttttat ggttaggagc atattatgtg atgagtggtg
     6241 agatgagcat aggagcactg attacttata acgcgttatt agttttcttt acagaaccgc
     6301 ttgagagtgt tattaattta caggtacaaa ttcaaaaagc acaagtagct agtcgacgaa
     6361 tgaatgatat actatcgata gaagttgaag gaaatattag taacgataca gttgaaattg
     6421 aagaaatggg agaagcagat ttaaagtttg acgatgtata cttctcatat gggcttggtg
     6481 aattgatact aagaggcata acatgtgaaa ttaaagtggg tgataaaata gcattagtgg
     6541 gtgaaagtgg atcaggtaaa tctacgttag ctaaaatgtt ggttcggttt tatgacccct
     6601 ctatgggaaa tatttatttc ggaaagtatg atattaatac aattgatgtg catattttaa
     6661 gacataatat agtatatgtt ccacaagatt cattcttttt ggcaggaacg attcgagaca
     6721 acttgttatt tggattagac tacgagccga ctcaaaatca aattgaacaa gcttgtgaaa
     6781 tagcgtgtat taaatcattt atagaatcat tacctctgca atatcatacg attgttgaag
     6841 aagcaggatc aaatttatca aagggacaaa aacaacgctt agcgattgtt agagcattac
     6901 ttacgaactc acaagttatt atttttgatg aagcaactag tggtatagat gtcttctccg
     6961 agcaaagaat aatggataat attataaaaa tgacagaacg tacgataata tttattaccc
     7021 atagcttacc tgcggctaaa ttatgtgaga aaataatggt tatggatcaa ggagatttag
     7081 ttgaagaagg aattcatgaa gtgttgagat atgctggagg aacgtatcaa aaattatggg
     7141 aagttatttc ttagctagtg aattgaagat aaaagccgtt acaattatac ttcataagta
     7201 gttgtaacgg cttttatatt agtttttatt tgggttagaa aatttttctt tgattgattt
     7261 agcacgtctg aaagcatact gaataatatg attgtctttt aggacaatat cacgattttt
     7321 ataattgata gacacaacat taccagggtt aattaatgct gatttggaaa tacggaataa
     7381 ttcagggaaa ttttcttcgc actttattaa acttccagaa aattcatatt cacccaatgt
     7441 agtggtcagc gttaatttgt gtgattcttc aatactagtc tgtaggtata aaactttctc
     7501 aaaatcaata ttaactgtct ctccatatct agaaaaagta aataattgtt ttttttcgtc
     7561 aagagaggta gttaatctat tatatgcaat gttcagtgtt tcaaaaatat ttttgcgtaa
     7621 ttcctctaca ttagacggtt tttcaatgaa tgacatagct tctaaattac gcttaaatgt
     7681 taaaggagca gcgtgttcat ccgttgtaat aaatactatt tttgcattta catcattttt
     7741 acgaatggct tctgctagat ctaacccgtc gattgtacta tttaggtgaa catctaacaa
     7801 aaagactcct tttttgggtt gatatgtttc taaataatct aaaacttcgt gtggagaaga
     7861 tgttattaat gacacattaa aacgagattc ataaaagcct gtataaaggt taataatttg
     7921 tttgattgcg tttaattgac cgattgagtc ttcgcaaata atgagtggat acattggctg
     7981 aatcctttca attataattt ggtagtaata atttttgcag aaaaaatggt gttcttttcg
     8041 aataacactg tgagattatc atgttttttc tcaattttag ttattgttga taatcccatt
     8101 ccgcgatcat tccctttcag agaaaatcct tcttgtttaa aattacggag ggtgtaatga
     8161 tcaggtatgt agggattaga aataacaagt tccatcactt tggtgtggct agtaatcaat
     8221 atatcaatat gtttttcttc aattgtcgta acgtattctt gtgcattatc taataggata
     8281 ccgaccacac gaatgaaatc aaatgtatct atatatatat tatctagtgg cttacgacat
     8341 tcaaattgac aggtaattcc caattcggta aaggtaacta atttagctag aataatactc
     8401 tttagagcag gatgtttgat attatgaata tctttatatt gaaatgttga gtgactgaaa
     8461 tagtctttgg aataatcgat taatgaatac atcatttctt tcagaccagc taagtcatta
     8521 ttttcaataa aactggtggt ggatagcatt aaatttttat agtcgtgttg aaattcttta
     8581 gttttatagg tttcttcttc gacaatatga ttatatttag tgagtgcttc taaatgttga
     8641 ttggatagtt cgtgttggaa ttttaattgt aaatatttgt gtaaagcata taaaaaaact
     8701 acagtaatga ttacaaatat gatagttatg gtaagtgtaa aataaagaag tttacgatac
     8761 aaatcaaacc attgaataaa gataaaatat gatgagaata aaatattgag gattaatagt
     8821 gagtatgtta ctattttttt ggccttaact tggtcgatat tggatgtaaa atatgggtag
     8881 atttttttat aaatagcaat tgacacaata aacgttagta tctttgtagt aatcatgact
     8941 aatgtgtggt tatgtaaatt gtaatatttt agggtgttga tgggtagagc aaccaagaat
     9001 tctacaattc ctttggtata aacgagtaga agaattgaat taagtacttc gaaatttatt
     9061 cttttcaacg aaaaaaatat gccatacaac actaaaccat atagtaaagc cacaatatca
     9121 gtggcataaa aaagatcatg tataatcata gaaaaaatcc aaacaacggg aaccgatata
     9181 cttacaaaaa ttctctttaa gacaggttta ttttgagtga ataaaaaatt gaacgtagtg
     9241 aagaacaaag atacgtcaag caatatttca taaataaata gaatgattgg attaacaaac
     9301 ataaaaacac ccttataaat tatctatata agggtatttt atcatgttat tcatatttat
     9361 gtctagcatc ttttatgttt tgacagcaaa cagttaaata tagcacgatg tgtatatgtt
     9421 ttattactat aaccaccgta aattttacgt aattcacgtt ccttaagtac cttaatgttc
     9481 ttttttctaa ttagatttga cattaaattc tcctccaaat aataattata tatagattaa
     9541 cattcatggt ggtcacggta atgtagtcat cataaacgta gtaataatgc tcctaaccgt
     9601 tagggtgttt aacaaatatt acgtttagga gcgaaaaata aacgaataag aaagaatacg
     9661 ttgtgtcata tgaatgacta gtatactagt attatcaact gacaaagaag gagggaaatg
     9721 aagtgaggcg attatatcag cgatatatga acaaaaaacg tttgaattct tggacaatat
     9781 atgcgagatc ttacacaagt aaaatgaagt tttaagaaaa ctgtacgttt aggagccaaa
     9841 agataacgaa taggaaaaaa ttctttctaa ctaacacaag aaaggtatta taaattatag
     9901 acggaaggga gggaagaaaa tgttaaaaac attaagaaaa ttatttagcc gtaatcagat
     9961 taatttgggt agattctact taggttataa aggaaaaaat gtgaagtggt aattatttag
    10021 agaaattaca gtgattattt gaatgtaagg taatctattg agtaaaagaa tagattacct
    10081 tacatctaat acatagaaag gaatatatga gatatacgaa taaagtgttt atgtttcatg
    10141 agataataat ttgtatagca tgtgtgtttg tagctgggtt gctggttaac attggaaatg
    10201 ttttgacaat aattttgcca tttggagata aagaattact aaatgttttg attttgtcaa
    10261 ttacaagtat ggcaggattt attttatttc cttataattt gtataaaaca atgtattctg
    10321 aactaaatca accaaaaata agctataaaa gagctattat tttatttata ggagctataa
    10381 taaccggatt atttaccgtt agtatatttg aaataattca accgtttttg atagcagcag
    10441 gagaggaatt tttatttcga tttattatat ataaattatt acagaataaa ttttctaaat
    10501 tccaaagtat gattgtaggg tcaattatat tttcaatagt gctacatcta aatggaaatt
    10561 tgttatttaa cttagtgact aaatttccga taagcatgct gttgtattat gtatatgaaa
    10621 agtatggact acaagattgt attgctattc attggttaca taatatgtta attgggaaat
    10681 ttgtataagg agtaagtaaa atgtatagaa aaatattatt gtcacttatt agtacattga
    10741 taatttttgg tacagggatt tatttaaatg tatcaatgtc tcttttgatg tttgctgtaa
    10801 tatcatatat attaccgtta atgggcaata tagtaattga ttattatgta caaacagaga
    10861 atgtattaat aggaagtggt atcatttcta cgattacaac gacaggttat gctatttttg
    10921 cgatactgat ggaaaataat atcaattttg caggatttat tagacagcat acatatcgta
    10981 gcgggaatat gacaatggga cttgaaccgg gattagcgga tatcagtcaa ttaattttta
    11041 ttttcgcatt gaatctatcc gtattatatt tcatacaata tttaagtaga ggagattttt
    11101 cgcatgttag acgtaagtaa tgtaagcaag caatatagtg gtaatgattt ttattcacta
    11161 aaggacgtca gttttacgat agaaaaagga gaaattgtcg gtttaatcgg aaagaatgga
    11221 gcggggaaga cgacattact gaagttgtta gcgaagtctc acttcccaac ggctggagtg
    11281 attcggtatc aagggctgga tatattctca aaagataatg tcttgcgtcc atttgggttg
    11341 atgattgaga cggtttatta cagtcattta acagtgaaac aaaatataga attttactta
    11401 gatattcacg atcaatcaga atataagaaa aatattcagt ccgtactgga aacagttgag
    11461 ttatggcaca agaaagattt taagccagat gggttctctt ttgggatgaa acagcgcttg
    11521 tcactggcaa tgagtttagt gacgaatcct gaatttatga ttttagatga accatttgta
    11581 ggattggatc cagatggcgt gaatgatctg atgcgtattt tgaagcagtg ggctgcagat
    11641 cgtggcacat caattattgt ctcaagtcat caattgaatg aattagaagc catttgtgat
    11701 cgttatttgt acattgaagg tggtgcactt aagaaacaat ttggcaaagc agctgttgaa
    11761 gtcactgtga ttgaattggt cgatgcactg caagacagag acgcgttatt cagtgcatat
    11821 gaagctattc aggccatctc agaagacggc aaacaaattg aagtcgattc aaatagtgaa
    11881 gccttccacc aaattttggg gactattaca gctagccata atatcaaaaa gattttcacc
    11941 aaagaaaatg cattaaatca atatttcaaa gggagcagag accatgagta aacttaacca
    12001 agttgtattt cacaatacat tgcgcaaaaa agaagtatgg gtatatctag catttaccct
    12061 tctgccactc cttgtttttg tgacggaatt aacggatacg aaattcttac gcttaacggg
    12121 cgatttttcg gagatgaact ttcttgattt ttat
//
