LOCUS       NZ_CP031264            10939 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Peterkaempfera bronchialis strain DSM 106435 chromosome, complete
            genome.
ACCESSION   NZ_CP031264
VERSION     NZ_CP031264.1
KEYWORDS    RefSeq.
SOURCE      Peterkaempfera bronchialis
  ORGANISM  Peterkaempfera bronchialis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Peterkaempfera.
REFERENCE   1  (bases 1 to 7090212)
  AUTHORS   Nouioui,I., Klenk,H.P., Igual,J.M., Gulvik,C.A., Lasker,B.A. and
            McQuiston,J.R.
  TITLE     Streptacidiphilus bronchialis sp. nov., a ciprofloxacin-resistant
            bacterium from a human clinical specimen; reclassification of
            Streptomyces griseoplanus as Streptacidiphilus griseoplanus comb.
            nov. and emended description of the genus Streptacidiphilus
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 1047-1056 (2019)
   PUBMED   30741626
REFERENCE   2  (bases 1 to 7090212)
  AUTHORS   Batra,D. and Gulvik,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2018) Bacterial Special Pathogens Branch, Centers
            for Disease Control, 1600 Clifton Road, Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 7090212)
  AUTHORS   Gulvik,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2018) Bacterial Special Pathogens Branch, Centers
            for Disease Control, 1600 Clifton Road, Atlanta, GA 30329, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: HGAP v. 3; Pilon v. 1.22
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 176.4x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003258605.2-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 16:52:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,114
            CDSs (total)                      :: 6,019
            Genes (coding)                    :: 5,833
            CDSs (with protein)               :: 5,833
            Genes (RNA)                       :: 95
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 60 of 186
            Pseudo Genes (incomplete)         :: 147 of 186
            Pseudo Genes (internal stop)      :: 2 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:20:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 337754
            Orig. end                         :: 348693
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031264.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10939
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10939
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10939
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5939](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5939
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(502..1233)
                     /locus_tag="C7M71_RS01430"
                     /old_locus_tag="C7M71_001430"
     CDS             complement(502..1233)
                     /EC_number="3.5.1.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014625.5"
                     /locus_tag="C7M71_RS01430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C7M71_001430"
                     /product="PIG-L deacetylase family protein"
                     /protein_id="WP_111493977.1"
                     /sec_met_domain="PIG-L (E-value: 5.2e-27, bitscore: 93.6,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRILAIGAHPDDIELGCGATLLRARRAGASVTLLVLTDGRLGPGD
                     PELRAHEQRQAADFLGAELVGGGFRDGELDHRPALVNRIEEVLDAVRPDLVFTHAPADS
                     HQDHRAAATATVAAARTLATVLHYETPSTLDFQPVVFGDITGLVPEKLRLLRHHLSQVI
                     GGRRIDLEALSAQARFRGFQGRVTEAEGFTATRALLGLAAPASPGGPVDRPVTVPTHRP
                     APDESAGVSGIGGIGGIGAEV"
     gene            complement(1230..2408)
                     /gene="wecB"
                     /locus_tag="C7M71_RS01435"
                     /old_locus_tag="C7M71_001435"
     CDS             complement(1230..2408)
                     /EC_number="5.1.3.14"
                     /GO_function="GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="wecB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Epimerase_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133975.1"
                     /locus_tag="C7M71_RS01435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C7M71_001435"
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="WP_111493975.1"
                     /sec_met_domain="Epimerase_2 (E-value: 2.5e-80, bitscore:
                     268.5, seeds: 187, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERHPAGPPGRNLPGRALRAVLFAGTRPEIVKLAPVARALSAAGA
                     HTELVATGQHHDHRMAGAFFADLGLAPAQRWTLDGDEAARVGTLLTRALRYFADHRPDV
                     AVVQGDTWTVPLVALAARRHGIPVAHVEAGLRSRNPLSPEECHRTAAADLATVHLAPTE
                     GARANLLREGIPDHAVHLVGNTVCDVLRTSGVAPVPPARRTGILVTAHRSGTVDAPDRL
                     AALVGIVRALADTRAPVTFPLHPRTADRLAATGLATRLDHPRITLLAPQPHRRMLELVA
                     GSALVATDSGGLQEEAGWFGVPAVVLRETTPRPEGVALGQAVLAGLDRDRALAAATALL
                     APAAQLRIAEMPCPYGDGHAAERIAALLTDHHSTGRLPLREPGPRTPEEAPR"
     gene            complement(2408..4084)
                     /locus_tag="C7M71_RS32430"
                     /old_locus_tag="C7M71_001440"
     CDS             complement(2408..4084)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133976.1"
                     /locus_tag="C7M71_RS32430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C7M71_001440"
                     /product="metallophosphoesterase family protein"
                     /protein_id="WP_265737636.1"
                     /transl_table=11
                     /translation="MDLPGFRVSRRRRDEMTIAEQYEYWQDVKKSLPRRRFLQFGAAGA
                     AALAVGPTLLSQAAYAEAPGSAVTPFGRHLAFGNDPRKQMRVAWQVPAAVKNPVLRLGT
                     SADDLSQVVQGELRNLHSDYGTSAPLDQYYGHAALDGLAPGTTYYYAIGHDGLEAAGGP
                     VKSFTTAPDAEGGALRPFTFTAMGDQGASAQGALENAQITAQNPVFHLLAGDICYADSS
                     GSGKLTDSYKPKVWDDYLTQIEPVAQSVPWMVATGNHDMESWYSPNGYGGHAQRLDLPT
                     NGPSNCPSVYAFTYGNVAVLSLDANDVSNEIPANRGYSGGSQTAWLDRTLAGLRADPKI
                     DFIVVFFHHCAYAVTTNHASDGGVRSAWTPLFDKYTVDLVVNGHNHMYERTDPIRNGSA
                     TRTASVGDTVSPVEDGTTYVVAGGGGASLYSLPANGAESYAGNVRDVSGVSGYAWNASG
                     AKATEAVDWSRVRYRGHNLLAVDVKPAAPGQTATLTVRAVSVEGDKAGTEVDRLTITRK
                     TATVDTPAFGMPAALTGAALAAGAGYVAWQKHRSGLEVPAS"
     gene            complement(4234..4887)
                     /locus_tag="C7M71_RS01445"
                     /old_locus_tag="C7M71_001445"
     CDS             complement(4234..4887)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406835.1"
                     /locus_tag="C7M71_RS01445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C7M71_001445"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_111493971.1"
                     /transl_table=11
                     /translation="MQQRAETTRRSVLEAAAHLFEERGYAGTSISDISALSGRTSGAIY
                     FHYNGKESLALAVVEEHFATWPQLIDRYATGSEPALDRLVRLSYAVAEAFRDDVVVRAG
                     ARLWAERKAIDAPLPTPFVAWLDTVTALLRQARSGGELAPHVDPEAAAHGLVCAFFGLH
                     TVSDALDGRRRITDHLGRLWLFLLPALQAHPAPAALLARVRDEGRAAVRGGALG"
     misc_feature    complement(4928..4948)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 18.84"
                     /tool="antismash"
     misc_feature    complement(4970..4984)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.19"
                     /tool="antismash"
     gene            5001..5939
                     /locus_tag="C7M71_RS01450"
                     /old_locus_tag="C7M71_001450"
     CDS             5001..5939
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056766.1"
                     /locus_tag="C7M71_RS01450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C7M71_001450"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_111493969.1"
                     /sec_met_domain="AfsA (E-value: 5.4e-21, bitscore: 72.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHGWDGLCRPGSRRPRPLSWSRTVPRELVHRTSVAEVLLTDVRPQ
                     GPGRFLAAAQWPRSHPTFPHGGDDRHSPLMIAETLRQLGIYLPLRYYGVPPDAHLLITD
                     LSFDLDPRAEPRAGHGATEVTCLIEVGGVRPAGGAGTPSGLRLRTRLLAGGRPFATAEG
                     GARFLDAGQYTALRAARADRPTGPPGRTPPVRPEPGRLAVASPRDVVLALAGDQLLVDP
                     ADPRHPYYFDHDTDHVPGMALLEAARQAAALRSGGRLLRPTGCRLRALRITEFRPPAPL
                     ECTVHHRSCVFRVRQGGAPTAVGVLRYARTR"
     gene            6226..6918
                     /locus_tag="C7M71_RS01455"
                     /old_locus_tag="C7M71_001455"
     CDS             6226..6918
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151397.1"
                     /locus_tag="C7M71_RS01455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C7M71_001455"
                     /product="HAD family phosphatase"
                     /protein_id="WP_111493967.1"
                     /sec_met_domain="HAD_2 (E-value: 1.2e-25, bitscore: 89.1,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTERIYAWTPAAVVFDCDGTLMNTENHWQEARELVLRSYGVAPD
                     AAFTARARGVHYTECGRLMAETAGQPGLAQEITDQLLENFRKLVADNPATLPGAAELVR
                     LTAAFAPLAVASNCPRDVVESSLGQAGLLDHFAHIVVPEDGTLPKPHPDVYLTAARRCG
                     ADPGDTLAVEDSPCGILAAVRAGLRVLGVGPYPEAEVAGLVDLWLPSLAEPDLLAWAAS
                     RTPRPSAR"
     gene            complement(6952..7623)
                     /gene="dhaL"
                     /locus_tag="C7M71_RS01460"
                     /old_locus_tag="C7M71_001460"
     CDS             complement(6952..7623)
                     /EC_number="2.7.1.121"
                     /GO_function="GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /GO_process="GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="dhaL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044382.1"
                     /locus_tag="C7M71_RS01460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C7M71_001460"
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="WP_114914126.1"
                     /transl_table=11
                     /translation="MAGHGGFDGAFTDRWLRRFAASATALEPELTALDQQVGDGDFGIN
                     LCAGLGAALAALDRLPPGGGPDAAAPLAAAATAFLDEVGGTSGPLFGLVFQELAAAAGP
                     ILTTPALAAGAAAGLAAVQRVGEAEPGDKTLVDALDPAVRELRRCPPDTLPDRALRAAS
                     DGAWEGVRSTARLRARRGRASYLGDRAVGIADPGAVGVGLLFASAGREVPALAPLLGGP
                     A"
     gene            complement(7638..8636)
                     /locus_tag="C7M71_RS01465"
                     /old_locus_tag="C7M71_001465"
     CDS             complement(7638..8636)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947698.1"
                     /locus_tag="C7M71_RS01465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C7M71_001465"
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="WP_111495539.1"
                     /transl_table=11
                     /translation="MAQYFANAPDTLVDDALAGFARAHAGLVVHDTGQAFVRARHGSPT
                     RRVGLLSGGGSGHEPLHIGFVGAGMLDAACPGRVFASPHNRQIFEASRAVARQEGVLHI
                     VKNYTGDRINFGIAAERLAHAGIACRRVLVDDDTASDSDDIAAGRRGTAATVLVEKVLG
                     AAADTGRGLDDLAELGASLASRCRTVAVASAAQTVPTTGRPAFALDPGLVEFGVGIHGE
                     RADRSLRQEPLGALVERMAAALLDALRPPPGTPLIALVNGLGAVTLLELYAAYNELARV
                     LEGSGLTVARSRVGNLVTALDMRGFSLSLLLADEETLALFDAPVQTPVWQW"
     gene            9429..9611
                     /locus_tag="C7M71_RS31280"
     CDS             9429..9611
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C7M71_RS31280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_229758462.1"
                     /transl_table=11
                     /translation="MQFALPHGEMLRLLRSHGFTVEDLIEIQAPQDVPRDFAEVSADCA
                     RNWPSEEIGKARLAD"
     gene            complement(9628..10917)
                     /locus_tag="C7M71_RS01475"
                     /old_locus_tag="C7M71_001475"
     CDS             complement(9628..10917)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005475582.1"
                     /locus_tag="C7M71_RS01475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C7M71_001475"
                     /product="ATP-binding protein"
                     /protein_id="WP_229758463.1"
                     /transl_table=11
                     /translation="MVAVSAWRFRDFHDGDLDQAIQVWDQSRQTDDRFPVFPVSEVMAA
                     ARSGQPAVVAVVGEELVGMAVAQVQGERAWVLLVALAARWRHRGIGSALLVELERRLRE
                     LGVRRISALLPESATGAKALENSGYGSRGGLTYFEKLEHLGPADAGLLAELGGRVMPAG
                     LWEALAGMEQEKRVVERRVVLPLARPDLADRHGVVPPKAVILFGPPGTGKTSFAKAVAS
                     RLGWPFVELFPSRLAASTGGGLAASLREVFADLAGLESVLLFIDEVEEIAGVRSGQAVD
                     PARGVTNELLKLIPGFREHEGRLLICATNSVRSLDSAFLRPGRFDYVIPIGPPDPAARA
                     AVWARYLGAAAATVDIARLVAASEMLTPADIEFAARKGAQAAFEQEVTEGRGEPAGTED
                     YLAAIADTRPTLTDQALAEFQEDIERYTRL"
ORIGIN
        1 ggttcgcagc cgagcaggga cagtgcccgg cggaatccgg aggggtgcgg tttgcgggag
       61 cggcggccct ggtcctcgtc ggtgcagatg acgccgctca gggcggcgcc gaggccggtg
      121 gcggcgagtt tggcccgctg ctgggccggg ttgccgtcgg tcagcagcac cagcggcacg
      181 gactcggcca gccgggccag ccgttcgggg acgccggggc agggggtgag cacggtcggc
      241 cggaacgcgc ggaatgcggc gaccagggcg gaggccaggg cggggtcgga cagtagctcg
      301 gcgagtgcgg cgtcgatcag tccgccccgg tcgctgccgg ccgaggcggc ccggtgcagg
      361 gcggcgcgca gcgcgtcccg gtccagcccg agggcggcac cggtgtcggc gacgtggtcc
      421 catgcctggt ccaggaactc ggcctggagg tagagggtgt cgtccaggtc gagcaggacg
      481 ccccgtgggg gccgggccat ctcagacctc cgcgccgatg ccgccgatgc caccgatgcc
      541 gctgacgcct gcgctttcgt cgggggcggg ccggtgggtg gggacggtga ccggccggtc
      601 gaccgggccg cccggggagg cgggcgcggc caggccgagc agggcccggg tggcggtgaa
      661 cccctccgcc tcggtgaccc ggccctggaa gccccggaag cgggcctgcg cggagagcgc
      721 ctccaggtcg atccgccgac cgccgatcac ctgggagagg tggtggcgca gcagccgcag
      781 cttctccggc accaggccgg tgatgtcgcc gaagacgacg ggctggaagt ccagggtgga
      841 gggggtctcg tagtgcagca cggtggcgag ggtcctggcc gccgcgacgg tggcggtggc
      901 ggcggcccgg tggtcctggt gggagtcggc gggggcatgg gtgaacacca ggtcggggcg
      961 gaccgcgtcc agtacctcct cgatccggtt gaccagcgcc ggccggtggt ccagctcgcc
     1021 gtcccggaag ccgccgccga ccagctcggc gcccaggaag tcggcggcct gccgctgctc
     1081 atgggcgcgc agctcggggt cgccggggcc gagtcggccg tcggtcagca ccagcagggt
     1141 caccgaggcg cccgcccggc gggcccgcag cagggtcgcc ccgcagccca gctcgatgtc
     1201 gtcggggtgg gcgccgatcg ccaggatcct catcgggggg cctcctcggg ggtgcggggg
     1261 ccgggctcgc gcagcggcag tcggccggtg gagtggtggt cggtgagcag cgcggcgatg
     1321 cgttcggcgg catggccgtc gccgtacggg cagggcatct cggcgatccg cagctgcgct
     1381 gcgggggcga gcagcgcggt ggcggcggcc agggcccggt cccggtcgag accggccagt
     1441 acggcctggc ccagggccac gccctcgggc ctcggggtgg tctcgcgcag caccacggcg
     1501 ggtacgccga accacccggc ctcctcctgg aggccgccgg agtcggtggc gaccagggcg
     1561 gagccggcga ccagctccag catccggcgg tgcggctgcg gggcgagcag ggtgatgcgg
     1621 gggtggtcca gccgggtggc caggccggtg gcggccagcc ggtcggcggt gcgcgggtgc
     1681 agcgggaagg tcaccggcgc ccgggtgtcg gcgagcgcgc ggacgatgcc gaccagcgcg
     1741 gcgagccggt cgggcgcgtc cacggtgccg gagcggtggg cggtgaccag gatgccggtg
     1801 cggcgggcgg gcggcacggg ggcgacgccg gaggtgcgca gcacatcgca gacggtgttg
     1861 ccgaccaggt ggaccgcgtg gtccgggatg ccctcgcgca gcaaattggc gcgggcgccc
     1921 tcggtggggg caaggtgcac ggtcgccagg tcggcggcgg cggtgcggtg gcactcctcg
     1981 ggcgagagcg ggttgcggga gcgcagcccg gcctcgacat gggcgacggg tatgccgtgg
     2041 cggcgggcgg ccagggcgac cagcggcacc gtccaggtgt cgccctggac cacggcgaca
     2101 tcggggcggt ggtcggcgaa gtagcggagc gcgcgggtga gcagggtgcc gacgcgggct
     2161 gcctcgtcgc cgtccagcgt ccagcgctgg gccggtgcca ggccgaggtc ggcgaagaac
     2221 gcgccggcca tccggtggtc gtggtgctgt ccggtggcga ccagttcggt gtgcgcgccg
     2281 gcggcgctca gtgcccgggc gacgggggcg agtttgacga tctcggggcg ggtgccggcg
     2341 aacagcaccg cccggagggc ccgcccgggg aggttcctcc cgggcgggcc tgcggggtgg
     2401 cgttccatca ggaggccggg acctccaggc cgctgcggtg cttctgccag gcgacatagc
     2461 ccgcgccggc ggcgagggcg gcgccggtga gcgcggcggg catgccgaag gcgggggtgt
     2521 ccacggtcgc ggtcttgcgg gtgatggtca gccggtccac ctcggtaccg gccttgtcgc
     2581 cctcgacgga gacggcgcgg acggtgaggg tggcggtctg cccgggggcg gcgggcttga
     2641 cgtccaccgc gagcaggttg tggccccggt agcggacccg cgaccagtcg acggcctcgg
     2701 tggccttggc gcccgaggcg ttccaggcgt agccggagac cccggagacg tcgcgcacat
     2761 tgccggcgta gctctcggcg ccgttggccg gcaggctgta gaggctggcg ccgccgccgc
     2821 ccgcgaccac ataggtggtg ccgtcctcca ccgggctgac ggtgtcgccg accgaggcgg
     2881 tccgggtggc gctgccgttg cggatcgggt cggtccgctc gtacatgtgg ttgtggccgt
     2941 tgaccaccag gtccacggtg tacttgtcga acagcggcgt ccaggcggag cgcaccccgc
     3001 cgtccgaggc gtggttggtg gtgaccgcgt aggcgcagtg gtggaagaag accacaatga
     3061 agtcgatctt cgggtcggcg cgcagcccgg ccagggtccg gtccagccag gcggtctggc
     3121 tgccgccgga gtagccgcgg ttggccggga tctcattgct gacgtcgttg gcgtccagcg
     3181 agagcaccgc gacattgccg taggtgaagg cgtagacgct cgggcagttg gacgggccgt
     3241 tggtgggcag gtccaggcgc tgggcgtggc cgccgtagcc gttgggcgag taccaggact
     3301 ccatgtcgtg gttgccggtg gcgaccatcc acggcaccga ctgggcgacc ggctcgatct
     3361 gggtgaggta gtcgtcccag accttgggct tgtaggagtc ggtgagcttg ccggagccgc
     3421 tggagtccgc gtagcagatg tcgcccgcga gcaggtggaa gaccgggttc tgcgcggtga
     3481 tctgcgcgtt ctccagtgcg ccctgggcgc tggcgccctg gtcgcccatg gcggtgaagg
     3541 tgaacggccg cagtgcgccg ccctcggcgt ccggcgcggt ggtgaagctc ttcaccgggc
     3601 cgccggccgc ctccaggccg tcatggccga tggcgtagta gtaggtggtg ccgggggcga
     3661 ggccgtccag cgcggcgtgg ccgtagtact ggtcgagcgg ggcgctggtg ccgtagtcgc
     3721 tgtgcaggtt gcgcagttcg ccctggacga cctggctgag gtcgtcggcg gaggtgccga
     3781 gccgcaggac ggggttcttg acggcggcgg ggacctgcca ggccacccgc atctgcttcc
     3841 tcgggtcgtt gccgaaggcg aggtggcggc cgaacggggt cacggccgag ccgggcgcct
     3901 cggcgtaggc ggcctgggag agcagggtcg gtcccacggc gagtgcggcg gcccctgccg
     3961 caccgaactg gaggaagcgg cggcggggca ggctcttctt gacgtcctgc cagtactcgt
     4021 actgctcggc gatggtcatc tcgtcgcggc ggcggcggga tacccggaaa ccgggaaggt
     4081 ccacggacct gcctttctcg gtggttcggg aagcggacgg acgcgttccg ctctgcatgc
     4141 agaggctgcg gggccgtggt gtcggacagg gtggtgcgag gtgaaccgat cggggtcgca
     4201 ccgtggagat caggtgaaga cgcccggccg ggctcagccc agggcgccgc cacggacggc
     4261 ggcgcggccc tcgtcccgta cccgggcgag cagcgccgcc ggggcggggt gggcctggag
     4321 ggcggggagc aggaagagcc agagccggcc gaggtggtcg gtgatccgcc ggcgcccgtc
     4381 cagcgcgtcg gagacggtgt gcaggccgaa gaaggcgcag accagaccgt gcgcggcggc
     4441 ctcggggtcg acatgggggg cgagttcgcc gccggaccgg gcctgccgga ggagggcggt
     4501 gacggtgtcg agccaggcca caaagggggt gggcaggggc gcgtcgatgg ctttgcgctc
     4561 cgcccagagc cgcgccccgg cgcggacgac cacatcgtcg cggaatgcct cggccacggc
     4621 gtagctgagc cgtaccagcc ggtccagggc gggctcggag ccggtcgcat agcggtcgat
     4681 cagctgcggc caggtggcga agtgctcctc gaccacggcg agcgccaggc tctccttgcc
     4741 gttgtagtgg aagtagatcg cgccgctggt gcgcccggac agcgcgctga tgtcgctgat
     4801 gctggtaccg gcgtacccgc gttcctcgaa caggtgggcc gccgcttcca gtacggacct
     4861 gcgcgtcgtc tccgcccgtt gctgcacgtg tccctgccct tgtccggtac gcccgctgac
     4921 gtcggggaat ctagccggtg cccgaccccc gaaaggggcg cggacatgcg attccctaaa
     4981 aagtgggatc ttctgtgcat atgcacggtt gggatggtct ctgccgtccg gggtcgcgcc
     5041 gaccccgccc gctgagctgg tcccgcaccg tcccccggga gctggtgcac cgcacctcgg
     5101 tggcggaagt cctcctcacg gacgtccgac cgcaggggcc cggacgcttt ctggccgccg
     5161 cccagtggcc gcgttcgcac ccgaccttcc cgcacggcgg ggacgaccgc cacagcccgc
     5221 tgatgatcgc cgagaccctg cgccagctcg gcatctacct gccgctgcgg tactacggcg
     5281 tcccgcccga cgcccatctg ctgatcaccg acctgtcgtt cgacctcgac ccccgggcgg
     5341 agccccgggc cgggcacggc gccaccgagg tcacctgcct gatcgaggtc ggcggcgtcc
     5401 gcccggccgg gggcgcgggc accccctccg gcctgcggct gcggacccgc ctgctggccg
     5461 ggggaaggcc gttcgccacc gccgagggcg gggcccgctt cctggacgcc gggcagtaca
     5521 ccgccctgcg cgccgcgcga gccgaccgcc cgaccgggcc gcccggccgt accccgccgg
     5581 tccggccgga acccgggcgg ctggccgtgg cgtcgccgcg cgatgtggtg ctggcactgg
     5641 ccggggacca gctgctggtg gacccggccg acccgcgcca cccctactac ttcgaccatg
     5701 acaccgacca tgtgcccggc atggccctgc tggaggcggc gcgtcaggcc gccgccctgc
     5761 gcagcggagg gcggctgctg cggccgaccg gctgccgact gagggcgctg cggatcaccg
     5821 agttccggcc gcccgccccg ctggagtgca cggtgcacca ccgcagctgc gtcttccggg
     5881 tcaggcaggg cggtgcgccg acggcggtgg gcgtactccg ctatgcgcgc actcgataag
     5941 caactctccg caatcggttg actacggttg gtggccggac gtagcgtagc gatcgttatc
     6001 ttctcgggtc gccccgcccg taccggcggc gcaccctgcg gctgcgcccg gccgagtcgg
     6061 ccctcggcgc cagcccccgc gcgagcggcc gaagccctcg gcgcatgtcc accaccccgt
     6121 ccccgcccgg ccgcccatgg ggcccgccgc cggtacggac tcctcccgat cgccccgcaa
     6181 cgccccccca cgaccctcgc cccctcgcac cctcatggag ggcacatgaa cacggaacgc
     6241 atctacgcct ggacgcccgc agccgtcgtc ttcgactgcg acggcaccct catgaacacc
     6301 gagaaccact ggcaggaggc ccgggagctg gtgctccgca gctacggggt ggcgcccgac
     6361 gccgccttca ccgcccgcgc caggggcgtc cactacaccg agtgcggacg cctgatggcc
     6421 gagaccgccg ggcagcccgg cctggcccag gagatcaccg accagctgct ggagaacttc
     6481 cgcaagctgg tcgccgacaa cccggccacc ctgccggggg cggccgagct ggtccgcctc
     6541 accgccgcgt tcgcgccgct ggccgtcgcc agcaactgcc cgcgcgatgt ggtggagtcc
     6601 tcgctcggcc aggccgggct gctggaccac ttcgcgcata tcgtcgtccc ggaggacggg
     6661 acgctcccca aaccgcaccc ggatgtgtac ctcaccgccg cccgccggtg cggcgccgac
     6721 ccgggcgaca ccctggcggt ggaggactcc ccgtgcggca tcctggccgc cgtacgcgcc
     6781 gggctgcgcg tcctcggggt cggcccctat cccgaggccg aggtggcggg cctggtggac
     6841 ctgtggctgc ccagcctggc cgagcccgac ctgctggcct gggcggcgag ccggacgccc
     6901 cggccgtccg cgcgctgagt gcccacggag gcgacggccg gtcggctggg gtcaggccgg
     6961 gccgccgagc agcggcgcca gcgcgggcac ctcgcgtccg gccgaggcga acagcaggcc
     7021 cacgccgacc gcgcccgggt cggcgatgcc caccgcgcgg tccccgaggt agctggcccg
     7081 gccccgccgc gcccgcagcc gcgcggtgct gcggacgccc tcccaggctc cgtccgacgc
     7141 ggcccgcagt gcccggtcgg gcagggtgtc cggcgggcac cggcgcagct cccgtacggc
     7201 cgggtccagt gcgtcgacca gcgtcttgtc ccccggttcc gcctcgccca cccgctgtac
     7261 ggcggccagt ccggccgccg cgcccgccgc cagcgcgggg gtggtgagga tcggtccggc
     7321 cgctgcggcc agctcctgga agaccaggcc gaagagcggg ccgctggtgc cgcccacctc
     7381 gtccaggaag gcggtcgccg cagccgccag cggggccgcc gcgtccgggc cgccgccggg
     7441 cgggagccgg tccagggccg ccagggcggc gcccagcccc gcgcagaggt tgatgccgaa
     7501 gtcgccgtcc ccgacctgct ggtccagcgc ggtcagctcc ggctccaggg cggtggccga
     7561 ggccgcgaac cgccgcagcc agcgatccgt gaacgccccg tcgaagccgc cgtgcccggc
     7621 catcccggcc gccccgccta ccactgccac accggggtct gcacgggggc gtcgaacagg
     7681 gcgagcgtct cctcgtccgc cagcagcagc gagagcgaga accccctcat gtccagcgcc
     7741 gtgaccagat tgccgacccg gctgcgggcc accgtcagcc cgctgccctc caggacccgc
     7801 gccagctcgt tgtaggcggc gtacagctcc agcagggtga ccgcccccag gccgttgacc
     7861 agggctatca gcggcgtacc ggggggtggg cgcagcgcgt ccagcagcgc cgccgccatc
     7921 cgctccacca gcgcgcccag cggctcctgg cggagcgagc ggtcggcccg ctcaccgtgg
     7981 atccccacgc cgaactccac caggcccggg tccagcgcga aggcgggccg accggtggtg
     8041 ggcacggtct gcgcggcgga cgcgaccgcg accgtacggc agcgggacgc cagcgacgcg
     8101 cccagctcgg ccaggtcgtc cagcccccgg ccggtgtccg cagcggcgcc gaggaccttc
     8161 tccaccagca ccgtcgccgc ggtgccccgg cgccccgccg cgatgtcgtc ggagtcggag
     8221 gcggtgtcgt cgtccaccag cacccggcgg caggcgatcc cggcgtgggc cagccgctcc
     8281 gccgcgatgc cgaagttgat ccggtctccg gtgtagttct tcacgatgtg cagcaccccc
     8341 tcctggcggg cgacggcgcg cgacgcctcg aagatctgcc ggttgtgcgg cgaggcgaag
     8401 acccggcccg ggcaggcggc gtccagcatg cccgccccga cgaagccgat gtgcaggggc
     8461 tcgtgcccgg aaccgccccc ggagagcagc cccacccggc gggtcggcga gccgtgccgg
     8521 gcccggacga acgcctgccc ggtgtcatgc accaccagcc cggcgtgggc ccgggcgaac
     8581 ccggccagcg catcgtccac cagcgtgtcg ggcgcattgg cgaagtactg ggccatcaag
     8641 cctcccacag tggttcggtg accgcagggg tcccgccgcg cccgacctgt cgtgcagcca
     8701 tcctgctgcc cagcaccggg cggtggcaac cgggtctgcg cccgcgccgg agcgcgccac
     8761 tgcgccgccg tccgcccggc ggccccctcg gcacccggcc gggcctgctt ccgggctgct
     8821 ccgcgcgggg ggacggcccg gcggacgggc ccgggaggag cgctccggcg cccgggaggc
     8881 cccgggcccg ccgcgcacct cgcggactcc gggggcgtac gcggcgggtc ggggcggcgg
     8941 gtcagggagg gttccggtgg cctgctcacg ggcgcgccgg ccgatggcga gggcgacggc
     9001 ggtcatggtg gcgatgccgc cgaaggagcc gcccagggcg gtcagcatct cccgggcgcg
     9061 gcggagggcg gccacctcgc catcgcctcg gccgtcgccg gtgtggacca gggcccggcc
     9121 gccgatggcc tgtacggagc ccgccggggc cgagccgtgg cggttcccgg tctccccgac
     9181 ctggggatat ccgccggacg gcggtcgacg gcagtttcga tctggcgatc agtgagttcg
     9241 gcgcctcgct ctggtgcgat ccctaccggt ggattccgga ggccgcccgg ctgctggctc
     9301 ccggcgggcg gctggtcttt ctgcgcttct ccccgctgta cgcgctctgt gcgcggccgg
     9361 acgggcaggc gtccgactcc ctgctccgcc cgcagttcgg cctgagccgg ctggattggg
     9421 agcggtcggt gcagttcgcc ctgccgcatg gggagatgct gcggctgctg cggtcccatg
     9481 gcttcacggt cgaggacctg atcgagatcc aggccccgca ggacgtcccc agggacttcg
     9541 cggaggtctc cgccgactgt gcccgcaact ggcccagcga ggagatcggg aaggcccggt
     9601 tggcggactg acggtacgcc atcgtgctca cagccgggtg taccgctcga tgtcctcctg
     9661 gaactcggcg agcgcctggt cggtgagggt gggtcgggtg tcggcgatgg cggccaggta
     9721 gtcctcggta ccggcgggct cgccgcgtcc ctcggtgacc tcctgctcaa aggccgcctg
     9781 ggcgcccttg cgggcggcga actcgatgtc ggccggggtc agcatctcgc tggccgcgac
     9841 cagccgcgca atgtcgacgg tcgcggcggc cgctccgagg tagcgcgccc agaccgccgc
     9901 gcgggcggcg gggtccggcg gcccgatcgg gatgacgtag tcgaaccggc ccggccgcag
     9961 aaaggccgag tccagcgacc gcaccgagtt ggtggcgcag atcagcagcc ggccctcgtg
    10021 ctcccggaag ccggggatca gcttgagcag ttcattggtc accccgcgcg ccgggtcgac
    10081 ggcctgaccg gaccggactc cggcgatctc ctccacctcg tcgatgaaga gcagcaccga
    10141 ctccagcccg gccagatcgg cgaagacctc gcgtagcgag gccgccagac cgcctccggt
    10201 ggacgccgcc agccgggagg ggaacagctc cacgaagggc catcccagcc gggaggccac
    10261 cgccttggcg aagctggtct tgccggtgcc cggcgggccg aagaggatca ccgccttcgg
    10321 cggcaccacc ccgtgccggt cggccaggtc cggtcgggcc agcggcagta ccacccgccg
    10381 ctccacgacc cgcttctcct gctccatccc ggccagcgcc tcccagagtc cggcgggcat
    10441 cacccggccg cccagttcgg cgagcagacc ggcgtcggcc ggcccgaggt gctccagctt
    10501 ctcgaagtag gtcagccctc ccctggaccc gtagccggag ttctccagcg ccttggcccc
    10561 ggtggcggac tccggcagca gtgcgctgat ccggcgcacc cccagctccc gcagccgccg
    10621 ctccagctcg acgagcaggg cgctgccgat cccccggtga cgccaccgcg ccgccagcgc
    10681 caccagcagt acccaggccc gctccccctg gacctgcgcc accgccatgc ccaccagttc
    10741 ctcgcccacc accgccacca cggcgggctg cccggagcgg gcggcggcca tcacctccga
    10801 cacggggaac accgggaacc ggtcgtcggt ctgccgactc tggtcccaca cctggatcgc
    10861 ctggtccagg tctccgtcgt gaaagtcccg gaaccgccat gccgacaccg cgaccaccgc
    10921 ctcgcttccg tcccggccg
//
