LOCUS       NZ_QJSP01000015        10903 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Williamsia limnetica strain DSM 45521 Ga0244495_115, whole genome
            shotgun sequence.
ACCESSION   NZ_QJSP01000015
VERSION     NZ_QJSP01000015.1
KEYWORDS    WGS; RefSeq.
SOURCE      Williamsia limnetica
  ORGANISM  Williamsia limnetica
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Williamsia.
REFERENCE   1  (bases 1 to 125756)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing
            the most valuable type-strain genomes for metagenomic binning,
            comparative biology and taxonomic classification
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 125756)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUN-2018) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Expected Final Version            :: No
            Genome Coverage                   :: 169.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003217475.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 01:29:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,026
            CDSs (total)                      :: 5,970
            Genes (coding)                    :: 5,910
            CDSs (with protein)               :: 5,910
            Genes (RNA)                       :: 56
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 17 of 60
            Pseudo Genes (incomplete)         :: 43 of 60
            Pseudo Genes (internal stop)      :: 7 of 60
            Pseudo Genes (multiple problems)  :: 7 of 60
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:55:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 82223
            Orig. end                         :: 93126
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QJSP01000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10903
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10903
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10903
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5903](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5903
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    495..510
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.52"
                     /tool="antismash"
     gene            1224..1979
                     /locus_tag="DFR67_RS22825"
                     /old_locus_tag="DFR67_11577"
     CDS             1224..1979
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DFR67_RS22825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DFR67_11577"
                     /product="hypothetical protein"
                     /protein_id="WP_110471781.1"
                     /transl_table=11
                     /translation="MLLPPVQALADRDVAKLLYTLTGVIDPALAAVEHLDPLGIKRRTF
                     STVERQANQAMSSEALITKVIGAIDDSLVWASDNAGLPGTARWASWTAEERRDWWIKRV
                     GPISTVAVAYPGVFGIIADRLPLQTVIGFAQQAVLLCAVARAYGVDDRHRHVDLLARVL
                     LNRNLDSKAVLSTTDAHTHASVSNDLARGAWNGIGKSRSILSELARRPSSRQPWRLLGA
                     LPVVGAAADYIGETGALRRAADNAVKNIR"
     gene            complement(2008..2388)
                     /locus_tag="DFR67_RS22830"
                     /old_locus_tag="DFR67_11578"
     CDS             complement(2008..2388)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006359466.1"
                     /locus_tag="DFR67_RS22830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_11578"
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="WP_110471782.1"
                     /transl_table=11
                     /translation="MSRRPKTIDEVLLEARAGLDRLAAQDLPAALAEGGVLVDIRPAAQ
                     RAEEGEHPAALVIERNVLEWRADPTSDARIPEAVDHDVAWIILCSQGYTSSLAAAALHE
                     IGLHRATDVIGGYEALAQAGLV"
     gene            complement(2435..3010)
                     /locus_tag="DFR67_RS22835"
                     /old_locus_tag="DFR67_11579"
     CDS             complement(2435..3010)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616645.1"
                     /locus_tag="DFR67_RS22835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_11579"
                     /product="cysteine dioxygenase family protein"
                     /protein_id="WP_245938103.1"
                     /transl_table=11
                     /translation="MTSTLSRPVAVGAPTQHQPRRTLSPTHHLPTRLRPADLLRLTDQG
                     ASDVLDGRHDDLLPRYWDREQRWATRLHYDDDVDVWLISWTPDESTDLHDHAGSLGALT
                     VLSGALTEYRWTGSQLRARTLEEGDQASFPLGWVHDVVRSPSRVVTSEPTLSVHAYSPP
                     LTAMSYYEVTDSQSLKRTKTVLTDGGAA"
     gene            3380..3706
                     /gene="fdxA"
                     /locus_tag="DFR67_RS22840"
                     /old_locus_tag="DFR67_11580"
     CDS             3380..3706
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="fdxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005244047.1"
                     /locus_tag="DFR67_RS22840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_11580"
                     /product="ferredoxin"
                     /protein_id="WP_084892184.1"
                     /transl_table=11
                     /translation="MTYIIAEPCVDVLDKACVEECPVDCIYEGGRMLYIHPDECVDCGA
                     CEPVCPVEAIFYEDDVPDEWAGYYNANVDFFDDLGSPGGASKLGKVDYDSPFVKALPPM
                     NTED"
     gene            3715..4830
                     /gene="dapC"
                     /locus_tag="DFR67_RS22845"
                     /old_locus_tag="DFR67_11581"
     CDS             3715..4830
                     /EC_number="2.6.1.17"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (37-369). E-value:
                     2.7e-37. Score: 120.6. Matches aSDomain:
                     nrpspksdomains_DFR67_RS22845_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="dapC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020105452.1"
                     /locus_tag="DFR67_RS22845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_11581"
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="WP_110471783.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4.9e-37, bitscore:
                     120.8, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVLRGRAPVSRRLPDFPWDTLLDAKERASAHPDGIVDLSVGTPV
                     DPVDPLIRDALAAASDFPGYPTTIGTPEVRRAAIDALQRRFGIDGLDLSMILPVIGTKE
                     AIAALPSTLGLGVGDQVVIPEVAYPTYEVGALLAGAEVVRADSLTQLGPATPAMIYLNS
                     PSNPTGKVLPVQHLRKVVSWARERGTVVVSDECYLGLAWDDEPVSILDTRVTDGDNTGL
                     LAVHSLSKTSNLASYRAGFLAGDPGLIGELLAVRKHAGMIVPFPIQAAMTAALGDDGHA
                     ATQRERYRRRRETLRNAVISAGFRIDDSAAGLYLWVTRDEPCRDTVAWLAERGILAAPG
                     DFYGPRGVRHVRMALTATDERIAQAASRLAS"
     aSDomain        3826..4821
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DFR67_RS22845_Aminotran_1_2.1"
                     /evalue="2.70E-37"
                     /label="DFR67_RS22845_Aminotran_1_2.1"
                     /locus_tag="DFR67_RS22845"
                     /protein_end="369"
                     /protein_start="37"
                     /score="120.6"
                     /tool="antismash"
                     /translation="DLSVGTPVDPVDPLIRDALAAASDFPGYPTTIGTPEVRRAAIDAL
                     QRRFGIDGLDLSMILPVIGTKEAIAALPSTLGLGVGDQVVIPEVAYPTYEVGALLAGAE
                     VVRADSLTQLGPATPAMIYLNSPSNPTGKVLPVQHLRKVVSWARERGTVVVSDECYLGL
                     AWDDEPVSILDTRVTDGDNTGLLAVHSLSKTSNLASYRAGFLAGDPGLIGELLAVRKHA
                     GMIVPFPIQAAMTAALGDDGHAATQRERYRRRRETLRNAVISAGFRIDDSAAGLYLWVT
                     RDEPCRDTVAWLAERGILAAPGDFYGPRGVRHVRMALTATDERIAQAASRL"
     gene            5001..5903
                     /locus_tag="DFR67_RS22850"
                     /old_locus_tag="DFR67_11582"
     CDS             5001..5903
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126479.1"
                     /locus_tag="DFR67_RS22850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_11582"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_110471784.1"
                     /sec_met_domain="DUF692 (E-value: 1.5e-64, bitscore: 210.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRSPVSGSSIAWRPAISDTLVELADHGSLTFTEVVAENMTPGRLP
                     IGVQELRSRGVVVIPHGVGLGLAGADLPNPERLAHLAALANELESPLVSEHIAFVRAAE
                     SPDPAHPDVLEAGHLLPPPRTRQALDILCDNISRAQDALGTPLALENIAALIGWPDDEF
                     DEPAYLAEIVRRTGVRLVLDVANLYASATARGDDPIKDLHRFPLAHIAYVHIAGGRFRE
                     GMYIDTHGDPMVDDVGSMLAAFVAQCRQGSQTPPGIMLERDSDVTAAAVLADLTTIDRI
                     ISSAHSTREEKSRDLEPST"
     gene            5881..6354
                     /locus_tag="DFR67_RS22855"
                     /old_locus_tag="DFR67_11583"
     CDS             5881..6354
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DFR67_RS22855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DFR67_11583"
                     /product="hypothetical protein"
                     /protein_id="WP_110471785.1"
                     /transl_table=11
                     /translation="MTSNPALDYARAALSARQHAVVTDLLAGRVPTGFDPVGSALTSDI
                     LIGKRASAALRAGPQLGALPQWRRRFAEYGREVAVSGCAHDDVAQFTRWLGGRPDLDPV
                     ARGWLDVEQVYSGARRSAWVRYRGRREWVIGIGSRTWHLAVSQVREGDEEGLS"
     gene            6606..6833
                     /locus_tag="DFR67_RS30960"
                     /old_locus_tag="DFR67_11585"
     CDS             6606..6833
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DFR67_RS30960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DFR67_11585"
                     /product="hypothetical protein"
                     /protein_id="WP_146240508.1"
                     /transl_table=11
                     /translation="MTVLVVVMACLAGGAVLGLIAIVGSRSSRAADRYRNDSGSSGGHS
                     DGWSGSSCSSDSSCSSSSCGSGCGGGGGGD"
     gene            complement(6927..7877)
                     /gene="dapD"
                     /locus_tag="DFR67_RS22870"
                     /old_locus_tag="DFR67_11586"
     CDS             complement(6927..7877)
                     /EC_number="2.3.1.117"
                     /GO_function="GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="dapD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020105453.1"
                     /locus_tag="DFR67_RS22870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_11586"
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="WP_110471787.1"
                     /transl_table=11
                     /translation="MTNASGASAIGIATITDSGAVLDTWYPAPELTEAGESGTTELDAA
                     DTPEELSSLVGTDADRGVRTVAVRTSIVLADAPTDAHDAYLRLHLLSHRLVIPHGANMD
                     GVFGKLSNVVWTNFGPCAVDGFELTRAKLKSRGAVTVYGIDKFPRMVDYVVPSGVRIAD
                     AGRVRLGAHLADGTTVMHEGFVNFNAGTLGQSMIEGRISAGVIVGDGSDVGGGASTMGT
                     LSGGGKEIISLGKRCLLGANSGCGIALGDDCVIEAGLYVTAGTKVTGPDGTVVKARELS
                     GKSNILFRRNSTTGAVEVVPWKGDGIELNAVLHAN"
     gene            7925..8638
                     /locus_tag="DFR67_RS22875"
                     /old_locus_tag="DFR67_11587"
     CDS             7925..8638
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /locus_tag="DFR67_RS22875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_11587"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_158540013.1"
                     /transl_table=11
                     /translation="MSFASTYKKRRSGSVPATLWRRGGQRGSAQVLPDGCMDLIWTGTG
                     LLVAGPDTGPVEVSGGADMVALRFDPGVAPSVLGVAADEVVNGRIDFADIAGSCRARKL
                     SEEMAAGEPELVLEQFATTQLDRRPPPRWLHPATALLSAGHPVASVADQINASSRQLQR
                     WSRHHYGYGPKTLQRILRVNAAMTGLRAGRTAVDTAHDCGYSDYAHMFRDFRAVTGRSP
                     ADFIPPADDGLFWRS"
     gene            complement(8676..9092)
                     /locus_tag="DFR67_RS22880"
                     /old_locus_tag="DFR67_11588"
     CDS             complement(8676..9092)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005183601.1"
                     /locus_tag="DFR67_RS22880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_11588"
                     /product="VOC family protein"
                     /protein_id="WP_110471788.1"
                     /transl_table=11
                     /translation="MTPPISRSSPTPSLNAISIIVDDMTETLTFYRACGLEFPEDAGAP
                     HAETTAAGGFRILFDTAASVATFDPDWRPATGGQRISLAFECAAPAEVDARYQALTAAG
                     HVGHLTPFDAVWGQRYATVSDPNGNTVDFYAALP"
     gene            9218..9862
                     /locus_tag="DFR67_RS22885"
                     /old_locus_tag="DFR67_11589"
     CDS             9218..9862
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005206760.1"
                     /locus_tag="DFR67_RS22885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_11589"
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_110471789.1"
                     /transl_table=11
                     /translation="MNTGSDSSWFAEIADERRTLAGTLATLTPEQWEAQSLCDAWTVRD
                     VAAHLVMPLVTPLWTFGLAMVRSRGDFDSANVRLTRRTCLRHGHRLPEMLVEHADSTFT
                     PPGHGPLAPLTDVLVHGQDIRRPLQIDYAIPEQRRRAVLDFLVGSSSASMFSAQTENLR
                     WQATDLDWGHGTGPIVEGPAAALMLVLTGRRVALMDTTGAGAELLAERKSR"
     misc_feature    complement(10211..10225)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.77"
                     /tool="antismash"
     misc_feature    complement(10561..10575)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
ORIGIN
        1 ctcgacgacg ccaccgtcct gctcggtgtg cggggcgaag acctcgccgc gttgtgcacc
       61 tacgcgggcc gtgtccgtga tgccggactg gaggcggagg gtcgcgccgg acagatcagt
      121 tactcccgca aggtgttcat cccgctgacc aggctgtgcc gcgaccgctg ccactactgc
      181 accttcgtca ccgtccccgg caagctggcc cgcgcgggcc aggggatgta cctggagatg
      241 gacgaggtcc tcgacatcgc tcgccgtggg gccgagatgg gctgtaagga ggcccttttc
      301 acgctcggtg atcgacccga agatcgttgg ccggaagcgc gggcatggct cgacgaccat
      361 ggatacacct cgactctcga ttacgtccgc gccgcctcga tccgcgtgct cgaagagacc
      421 gggctgctgc cacacctcaa tccgggtgtg atgagcagcg acgagatggc ctggctcaaa
      481 cccgttgcgc cgtcgatggg aatgatgctc gagaccactg cccgcagatt gttcaccgac
      541 aagggcaacg cgcactacgg cagtccggac aaagaccccg agatccgcaa gcaggtgctg
      601 atcgacgccg gcaagaactc tgtgcctttc acgaccggca tcctggtcgg aatcggggag
      661 aaccgggccg agcgcgccga ttcgctgttc gagatggcga aggtgcacaa cgacttcggg
      721 cacctgcaag aggtcatcgt gcaaaacttc cgggcgaaac cggacaccgc gatgcgcggg
      781 accgccgatg cggagttcga cgagttcctt gccgctgtcg cggtggcgcg actcgttctc
      841 gggccacaca tccggatcca ggccccgccg aatctcgtgt caccggaaga gtgcgctgcg
      901 ctggtcggtg ccggtgtgga tgactggggc ggcgtatcac cgctgacccc cgaccacgtc
      961 aaccccgaga ggccgtggcc caacctcgat gcgcttgccg agatcaccgc aggcaccggc
     1021 tacgtgctca ccgagcgtgt tgcggcgcaa ccgagatacg tgttgaccgg cgagccgtgg
     1081 atcgatgacc ggatcagcgc gcacgtccgt gctctggccg accccgaaac cgggctggcc
     1141 gtggaggtcc acccggtcgg aatgccgtgg catgacaacg cagaacctgc cgcgacgctc
     1201 accggcacga cacaggtccg ttgatgctgc tgccacccgt ccaggctctg gccgaccgcg
     1261 atgtcgccaa gctgctctac accctcaccg gtgtcatcga cccggcgctc gccgcggtcg
     1321 agcacctcga cccactgggc atcaaacggc gcaccttcag cacagtcgaa cggcaggcga
     1381 accaggcgat gtcgtccgag gcgctcatca ccaaggtcat cggtgccatc gatgattcgc
     1441 tggtctgggc gtccgacaac gcgggtctgc ccggaaccgc caggtgggcg agctggaccg
     1501 cggaagaacg tcgggactgg tggatcaaga gggttggccc gatcagcact gtggccgtgg
     1561 cgtacccggg cgtcttcggc atcatcgccg atcgtctccc gttgcagacc gtcatcggct
     1621 ttgcccagca ggcggtgctc ctgtgcgcgg tcgcccgcgc atacggcgtc gacgatcgcc
     1681 atcgccacgt ggatctgctc gcgcgcgtac tgctcaaccg gaatctcgac tcgaaagccg
     1741 tgctcagtac caccgatgcg cacacacacg catccgtgtc gaatgacctc gcacgcgggg
     1801 cgtggaacgg gatagggaaa tcgcggtcca tcctctccga actggcccgg cgcccgtcct
     1861 cgcggcaacc ctggcgattg ctcggcgcgc taccggtcgt cggtgcggct gccgattaca
     1921 tcggggagac cggcgcatta cgtcgcgctg ccgacaacgc ggtgaagaac attcgctgag
     1981 cagagcgagc ttgtcggcca cagagggtca gacgaggccc gcctgagcca gcgcctcata
     2041 tccgccgatg acatcggtgg cgcggtgtag tccgatctcg tgcagcgctg cagcggccag
     2101 gctcgaggtg taaccctgcg agcacaggat gatccacgcg acgtcgtggt cgacggcctc
     2161 ggggatcctg gcatccgacg tcggatcggc gcgccactcg aggacgttgc gctcgatcac
     2221 cagcgcggcc gggtgctcac cctcctccgc gcgctgggcg gcagggcgaa tgtcgacgag
     2281 tacgcccccc tcggccagtg cagccggaag atcctgtgcc gcaagtcggt cgagtccggc
     2341 gcgggcctcg aggagaacct cgtcgatggt cttgggtcgc cggctcatgc cgcatcaccg
     2401 tcggtgagca ctgtcttggt ccgacgtccc gaactcatgc cgcaccaccg tcggtgagca
     2461 ctgtcttggt gcgcttgagc gactggctgt cggtgacctc gtagtaggac attgcggtca
     2521 acggcggtga gtaggcatgg acgctcaggg tcggttcgct cgtcacgacg cgactcgggc
     2581 tgcggacgac gtcgtgtacc cagccgagcg ggaacgaggc ctgatcgcct tcctcgagag
     2641 tgcgcgcccg caattgactc ccggtccagc gatactcggt cagtgcgccc gacagcacgg
     2701 tcagcgcacc gagtgatccc gcatggtcgt gcagatccgt ggactcgtcg ggagtccagc
     2761 tgatcagcca gacgtcgaca tcatcgtcgt agtggagccg ggtcgcccag cgttgctcgc
     2821 ggtcccagta tctgggcagc aggtcgtcgt gacgaccgtc gaggacgtcc gaggcgccct
     2881 gatctgtcag ccgaagcagg tccgcgggac gcagccgcgt gggcaggtgg tgggtggggg
     2941 acagggtgcg gcgcggctgg tgctgagtcg gcgcgccgac ggcgacgggc cgcgaaagag
     3001 tcgaggtcat gtgcggatgc tccttgagag tcgaaaggtg ttgggtggcc agaggtcagc
     3061 aacaacacat tcggttccac aggggggtcg gtcgcacggt cacggaacag atcctgccgc
     3121 cgctgaggcg ttgcgcacag gtttaggacc ggtctgattt gaaagccgtg gtcaggcgcg
     3181 cgcgtcggca tcgccccgcg ttgacctgga caggcgccgc agagcccagg tgacccgggt
     3241 tttgacttgg aatccagtac gcggatactg ggaggcaacg agcgggcagg ctgggcgacc
     3301 ttcaatccac aaggcatgcc cgctttcgag cgcgtgcagc ccacgcgtga gcgaactgtg
     3361 attgtgagga gggtggccgg tgacctatat catcgctgaa ccttgcgttg atgtcctgga
     3421 caaggcatgt gtcgaggagt gcccagtcga ctgcatctac gagggtgggc ggatgctcta
     3481 catccatccc gacgagtgtg tcgactgcgg tgcgtgcgaa ccggtgtgcc cggtcgaggc
     3541 catcttctac gaggacgatg tgcccgacga gtgggcaggc tattacaacg cgaacgtcga
     3601 cttcttcgac gacctcgggt cgccgggtgg agccagcaaa ctcggcaagg tcgactacga
     3661 ctcgccgttc gtgaaggcgc tgccgccgat gaacacagag gactgacgac gccgatgtct
     3721 gtgttgcgcg gtcgcgcgcc ggtgtcgagg cggctcccgg attttccctg ggacacgttg
     3781 ctcgacgcga aggagcgcgc gtccgctcat ccggacggaa tcgtggacct ctcggtcggt
     3841 acgccggtgg atcctgttga tccgttgatc cgcgacgcgc tggctgctgc ctccgatttc
     3901 cccggatacc ccacgacgat cggtaccccc gaggtgcgca gggcggcgat cgatgcgttg
     3961 cagcgcaggt tcgggatcga tggtctggac ctctcgatga tccttccggt gatcggcacg
     4021 aaggaggcca tcgccgcatt gccgtcgacc ctcgggctcg gcgtgggtga ccaggtcgtg
     4081 atacccgagg tcgcttatcc gacatatgag gtcggggccc tgctggcggg cgcggaggtg
     4141 gtgcgagccg attcgctgac ccagctcggt cccgcgacgc ccgccatgat ctacctcaac
     4201 tctccctcga acccaaccgg caaggtgctg cccgtgcagc acctgcgcaa ggtcgtctcc
     4261 tgggctcgcg aacgcggcac ggtagtggtg tcggacgagt gctacctggg cctggcctgg
     4321 gacgacgagc cggtctcgat tctcgatacg cgggtgaccg acggagacaa caccggcctg
     4381 ctggcggttc attcgttgtc gaagacgtcg aatctcgcga gctaccgggc cgggttcctc
     4441 gccggtgatc ccgggctcat cggtgaactg ctggccgtac gcaagcacgc cggcatgatc
     4501 gtccccttcc cgatccaggc agcgatgacg gcggctctcg gtgacgacgg ccatgcggcc
     4561 acccagcgcg aacgctaccg gcggcgccgc gagaccctgc ggaacgccgt gatcagcgcc
     4621 ggtttccgga tcgatgactc ggccgccgga ctgtatctgt gggtcacgcg cgacgagccg
     4681 tgccgcgaca ccgtcgcctg gctggcagag cgtggaattc tcgccgcccc cggcgacttc
     4741 tatggacctc gtggcgtccg gcacgtgcgg atggccttga cggccaccga cgagagaatc
     4801 gcgcaagcag ccagccgtct ggcctcctga cctgcgccgt cgtagctgtg tccaatctga
     4861 gaccagtccg ttaccccagg agccggtatt tcggtgaaac tgtggacaag caacagaact
     4921 gactgcgaat ccgccactcg gacaacggat tcgaaagtgg ccaacggcgt ccactcgtaa
     4981 gcgagtgaaa ggacgcgtag atgaggtcac ctgtatcggg ttcgtccatc gcctggcggc
     5041 cggcaatttc ggacactctc gtcgaactcg ccgaccacgg ctccttgacc ttcaccgagg
     5101 tcgtcgccga gaacatgacg cccggccgtc tgccgatcgg cgtccaagag ctcaggtcgc
     5161 ggggcgtggt ggtgatcccg cacggggtcg gactcgggct cgccggagcg gacctgccga
     5221 acccggagcg gctcgcgcat ctcgcggcac tcgcaaacga actcgaatcg cctctggtga
     5281 gtgagcacat cgcctttgtc cgggcagccg aatcgccgga tcccgcacac ccagatgtgc
     5341 tggaggccgg gcacctgctg ccaccaccgc gaacccgcca ggccttggac attctgtgcg
     5401 acaacatctc ccgcgcgcag gatgcgctgg gtacgcctct cgcactcgag aacatcgcgg
     5461 ccctgatcgg ctggcccgac gacgagttcg acgaacccgc ctatctggcc gagatcgtcc
     5521 gccgcaccgg tgtgcgactt gtcctcgacg tggccaatct ctacgccagt gccactgctc
     5581 gaggtgatga ccccatcaag gacctgcacc gtttcccgtt ggcgcacatc gcatacgtcc
     5641 atatcgccgg tgggcggttc cgggagggca tgtacatcga cacgcacggt gatccgatgg
     5701 tcgacgatgt cggttcgatg ctcgcggcgt tcgtcgcaca gtgccgccag ggttcccaga
     5761 ccccgcccgg catcatgctc gagcgtgaca gtgacgtcac tgccgctgcg gtgctggccg
     5821 atctgaccac catcgatcga atcatcagtt cggcacattc cactcgggag gagaagtcgc
     5881 gtgacctcga acccagcact tgactacgcc cgggcggcac tgtcggcacg gcagcacgcg
     5941 gtggtcacgg acctgctggc aggtcgcgtg ccgacagggt tcgatccggt gggctcagct
     6001 ctcaccagcg acatcctcat cggcaaacgc gcatcggcgg ccctgcgagc aggcccgcaa
     6061 ctcggcgccc tgccgcagtg gcgacgccgg ttcgccgagt acggacgtga ggtcgcggtc
     6121 agcgggtgcg cgcacgacga tgtcgcgcag ttcacgcggt ggctgggagg tcgccccgat
     6181 ctcgatccgg tcgcccgggg ctggctcgac gtcgaacagg tgtattccgg tgctcgacgg
     6241 tcggcgtggg tgcgctaccg cggacgacgt gagtgggtca tcggtatcgg ttcgcgcaca
     6301 tggcatctag ctgtttcgca ggtacgagaa ggtgatgagg aggggttgtc atgaccgttg
     6361 tggtgattgt gatcgtcgga gtggtcagcg tttcggtgct ctttttggca agggcatttc
     6421 acgacgacgc cggacccgac aaaggtcgcc gccggcgtcc aggcggctgg atcggggacg
     6481 gcagcggtgg tggtgacagc agtggatcgt gcggtagctc gtgtgggagt agctgcggag
     6541 gtggatcatc cggtggaggt ggcggtggcg gctgcggaag cagcggcgga tagggaggca
     6601 gcgcgatgac agttctcgtg gtggtgatgg catgtctggc gggtggggcc gtcctcggcc
     6661 tgatcgcgat cgtggggtcg aggtcgtcca gggcggccga ccggtaccga aatgattccg
     6721 gtagctcggg tgggcactcg gacggctggt ccggcagctc gtgcagctcc gattcgtcgt
     6781 gcagctcgtc ctcgtgtgga tcgggctgcg gcggaggcgg cggcggggac tgacaggaaa
     6841 ccacctcgca cctgaatacg caccgcccac cggtgacaac cggtgggcgg tgcgtatttt
     6901 ctgtggcggt gtgcgccggc gttcgatcag ttcgcgtgca gcacggcgtt cagctcgatg
     6961 ccgtcgccct tccacggcac gacctcgacc gcaccggtgg tgctgttgcg acggaacagg
     7021 atgttgctct taccggagag ttcgcgggcc ttgacgacgg tgccgtccgg tccggtgacc
     7081 ttggtccccg cggtgacgta gagcccggcc tcgatgacgc agtcgtcgcc gagcgcgatg
     7141 ccgcagccgg agttggcgcc gagcaggcag cgcttgccca gagagatgat ctccttaccg
     7201 ccgccggaca acgtccccat ggtggacgcg ccgccgccga catcggagcc gtccccgacg
     7261 atgacgcccg ccgagatgcg accttcgatc atcgactggc ccagcgtgcc cgcgttgaag
     7321 ttcacgaaac cctcgtgcat gacggtggtg ccgtcggcga ggtgcgcacc gaggcggacg
     7381 cgcccggcgt cagcgatccg gaccccgctc ggcacaacgt aatccaccat tcgagggaac
     7441 ttgtcgatac cgtagacggt gaccgcgccg cggctcttga gcttggcgcg ggtcagctcg
     7501 aaaccgtcga ccgcgcatgg gccgaaattg gtccagacca cgttggagag cttcccgaag
     7561 acaccgtcca tgttcgcgcc gtgtgggatg accaggcgat gcgagagcag gtgcagacgc
     7621 aggtaggcgt cgtgggcatc ggtgggggcg tcggcgagca cgatcgaggt gcgcacggcc
     7681 acggtcctga cgccgcggtc tgcatccgtt cccaccagcg acgacagttc ttcgggggtg
     7741 tcggcagcgt cgagttcggt ggttcccgat tcgcccgcct cggtcagttc gggggcgggg
     7801 taccaggtgt cgagcactgc gcccgagtcg gtgatggtgg cgatgccgat tgcggaggct
     7861 ccagatgcgt tggtcacggg tcaggagttt acgcggggtg atcgggggcg gaacaatgga
     7921 gcgcatgtcc ttcgcctcga cctacaagaa gcgtcgctcc gggtcggtgc ccgcaacgct
     7981 ctggcgtcgc ggtgggcaga gaggttcggc gcaggtgcta ccggacggtt gcatggacct
     8041 catctggacc ggcaccggct tgctcgtggc cggcccggac accggtccgg tcgaggtcag
     8101 cggaggcgcg gacatggtgg cgctgcggtt cgatcccggc gtggctccgt cggtgctcgg
     8161 ggtcgcggcc gacgaggtgg tcaacggccg gatcgacttc gccgacatcg cagggtcgtg
     8221 ccgggcgcgg aagctgtccg aggagatggc ggccggcgag cccgaattag tcctggagca
     8281 gttcgcgacg acccagctgg accggcgccc gcctcctcgg tggctgcatc cggccacggc
     8341 cttgctgtcg gcgggtcatc cggttgcaag cgtcgcggac cagatcaatg ccagttcgcg
     8401 gcagctacag cgctggtcgc gccatcacta cggatacggc cccaagacgc tgcagcggat
     8461 cctccgggtg aacgcggcga tgaccggtct gcgcgcgggc cgtacggccg tcgacaccgc
     8521 ccacgactgc gggtactccg attacgctca catgtttcgc gacttccggg ccgtgacggg
     8581 ccgctcgccc gccgatttca tcccgcccgc cgatgatggg ttgttctggc gaagctgacc
     8641 agggcttttc ttcgccagaa cgacccatta tcgggtcagg ggagcgcggc gtagaagtcg
     8701 acggtgttgc cgttggggtc gctgaccgtg gcgtaccgct gaccccagac cgcgtcgaac
     8761 ggggtgaggt gcccgacgtg accggcggcc gtcagcgctt ggtaacgggc gtcgacctcg
     8821 gcgggagcag cgcactcgaa tgcgagcgag atccgctggc caccggttgc gggacgccag
     8881 tccggatcga aggtcgcgac gctcgcagcg gtgtcgaaca ggatccggaa tccgcccgcc
     8941 gcagtggttt ccgcgtgggg ggctcccgcg tcctcgggaa actcgagacc gcaggcgcgg
     9001 tagaacgtca gcgtctcggt catgtcgtcg acgatgatgg agatggcgtt gagggatggc
     9061 gtcggagatg accgcgatat tgggggtgtc atggcgtcga cgttatcggc gagggcggtg
     9121 gcgcggcatg aacatttcgg acacgttgca gggccgacgg cggcgttgtc gaatggttac
     9181 gtccgaaccc tcccgatcag cctcgtagga gccccgtgtg aacaccggat cagacagctc
     9241 atggttcgcc gagatcgccg acgaacgccg cacgctggcc ggcacccttg cgacgttgac
     9301 ccccgaacag tgggaggcac agtcgctgtg tgatgcctgg acggtccgcg atgtcgcggc
     9361 gcacctcgtg atgcccttgg tgacgccgct ctggacgttc ggcctggcga tggtgcgatc
     9421 ccgcggtgac ttcgacagcg caaacgtgcg gctcacgcgt cgcacctgcc tccggcacgg
     9481 ccaccgattg cccgagatgc tggtcgagca cgcagacagc acgttcaccc cgcccggcca
     9541 tggcccgttg gcgccgctga ccgatgtcct cgtccacggc caggacatca ggcgcccatt
     9601 acagatcgac tacgccatcc ccgaacagcg ccggcgggct gtgctcgact ttctcgtcgg
     9661 ctcgtccagc gcgtcgatgt tctccgcgca gaccgagaac ctgcgttggc aggcaacaga
     9721 tctcgattgg ggtcatggca ccggcccgat cgtcgaaggg ccggcggcag ccctgatgct
     9781 cgtactcacc ggacgacgtg ttgcgctgat ggacacgacc ggtgccggcg ccgaactcct
     9841 ggccgagcgg aagtcccgtt gagtccgact cggccggaaa gcccaaacgc tacggtgaac
     9901 actgtgagca tccccgcact ggacctgcac gccgacccga tcgacctgac ggcggcgttg
     9961 gtcgacatcc ccagcgagag ccatgacgaa tccctcatcg cggacgccgt cgaagccgcg
    10021 ctccgcgagc agacgaccgg attcgaggtc gtccgccacg gcaaccgcgt gctggcccgc
    10081 accaacttcg gaaaaggtcg gcgggtgatg ctggccggac acctggacac cgtgccgatc
    10141 gccgacaacg tgccaagccg acgagagcag gacagctccg aaggcgacgt gttgcacgga
    10201 tgcggcacgg tggacatgaa gtccggcgac gcggtgttct tgcatctcgc ggcgaccctc
    10261 gaagaacccg tgtgtgatct gacgctgatc ttctacgact gcgaagagat cgccgcccgg
    10321 tacaacggtc tgggtttcat cgagcgggag ctacccgaat ggttgcgcgc tgatgtcgcc
    10381 atcctcggcg aaccgacagc gggccagatc gaggccggat gccagggcac gctgcgggcg
    10441 aggctttcgg cgtcgggtgt gcgatcccat tcggctcgct cgtggaaagg cgacaacgcc
    10501 atccacaaac tcgggtcggt gttgcagacc ctgtccgact acaccccgcg acgggtggac
    10561 atcgacggtt gtgaataccg cgaaggcctc tcggcggtac gcatcgacgg cggggtggcc
    10621 gggaacgtcg tgcccgacgt ggcgagcgtc gacgtgaact tccggttcgc cccggaccgc
    10681 tccgtcgagc aggccaccga tcacgttcgt gaggtcttcg ccgcggcaat cgattcgggc
    10741 gcggtcacct tcgagatcac ggacgcggca ggcggggcgc tacccggatt gtcagacccg
    10801 gcggccgccg cgctggtcgc cgcggccggc ggcaagttca gggccaagtt cggctggacg
    10861 gacgtctccc ggttctcggc cctcggtatc ccggcggtca acc
//
