LOCUS       NZ_QJSP01000010        10807 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Williamsia limnetica strain DSM 45521 Ga0244495_110, whole genome
            shotgun sequence.
ACCESSION   NZ_QJSP01000010
VERSION     NZ_QJSP01000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Williamsia limnetica
  ORGANISM  Williamsia limnetica
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Williamsia.
REFERENCE   1  (bases 1 to 280374)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing
            the most valuable type-strain genomes for metagenomic binning,
            comparative biology and taxonomic classification
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 280374)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUN-2018) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Expected Final Version            :: No
            Genome Coverage                   :: 169.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003217475.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 01:29:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,026
            CDSs (total)                      :: 5,970
            Genes (coding)                    :: 5,910
            CDSs (with protein)               :: 5,910
            Genes (RNA)                       :: 56
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 17 of 60
            Pseudo Genes (incomplete)         :: 43 of 60
            Pseudo Genes (internal stop)      :: 7 of 60
            Pseudo Genes (multiple problems)  :: 7 of 60
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:55:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 199502
            Orig. end                         :: 210309
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QJSP01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10807
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10807
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10807
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5807](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5807
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(893..2158)
                     /locus_tag="DFR67_RS17955"
                     /old_locus_tag="DFR67_110196"
     CDS             complement(893..2158)
                     /EC_number="1.2.1.41"
                     /GO_function="GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA]"
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /GO_process="GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011207901.1"
                     /locus_tag="DFR67_RS17955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_110196"
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="WP_110470855.1"
                     /sec_met_domain="Aldedh (E-value: 8.7e-19, bitscore: 61.4,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPTTTPADVEQIVRAQATASKAASRVLAGLTTDRKNRVLHDAA
                     DALLAQTGSILAANAEDVARAELAGTEESLIDRLRLTADRVEGIAAGLRQVAALPDPIG
                     DVIQGKTLPNGMALRQVRVPLGVVGMVYEARPNVTVDAFGIALKSGNSALLRGSSSAAS
                     SNAELVRILRDTLTAAGLPADAVQLLPSEDRSSVTHLIRARGLVDVVIPRGGAGLIKAV
                     VENATVPTIETGTGNCHIYIHAAADLDMAEDLVINSKTRRPSVCNTAETVLVDKAIAET
                     ALPRLTQVLQSAGVIIHGDEPGMVPATEEDWSDEYLSLDIAVKIVGGLDEAVDHIDRYG
                     TSHTEAIITDDLAAATEFTNRVDAAAVMVNASTAFTDGEQFGFGAEIGISTQKLHARGP
                     MGLPELTSTKWIAWGNGQTRPV"
     misc_feature    complement(1862..1864)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(2121..2136)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.74"
                     /tool="antismash"
     gene            complement(2314..3093)
                     /locus_tag="DFR67_RS17960"
                     /old_locus_tag="DFR67_110197"
     CDS             complement(2314..3093)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: ECH (13-206). E-value: 3.3e-46. Score:
                     149.5. Matches aSDomain:
                     nrpspksdomains_DFR67_RS17960_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078026.1"
                     /locus_tag="DFR67_RS17960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_110197"
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="WP_110470856.1"
                     /transl_table=11
                     /translation="MTASANVLVSHDGPITTISINRPQRRNAVDGPTAEALAAAFRDFE
                     GDDGAAVAVLHGEGGTFCAGADLKAIAAGEAPNRIDSAGDGPMGPTRMRLSKPVIAAIA
                     GHAVAGGLELALWADLRIAEEDAVFGVFCRRWGVPLIDGGTVRLPRLVGTSHAMDMILT
                     GREVRANEAHRMGLVNRIVSQGDALKSAKELAREIAAFPQTCMRQDRLSVLEQEGLDET
                     GAMDNEFRRGMTSLSADTVAGATRFAGGAGRHGSFEA"
     aSDomain        complement(2476..3054)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DFR67_RS17960_ECH.1"
                     /evalue="3.30E-46"
                     /label="DFR67_RS17960_ECH.1"
                     /locus_tag="DFR67_RS17960"
                     /protein_end="206"
                     /protein_start="13"
                     /score="149.5"
                     /tool="antismash"
                     /translation="PITTISINRPQRRNAVDGPTAEALAAAFRDFEGDDGAAVAVLHGE
                     GGTFCAGADLKAIAAGEAPNRIDSAGDGPMGPTRMRLSKPVIAAIAGHAVAGGLELALW
                     ADLRIAEEDAVFGVFCRRWGVPLIDGGTVRLPRLVGTSHAMDMILTGREVRANEAHRMG
                     LVNRIVSQGDALKSAKELAREIAAFPQTCM"
     gene            complement(3005..3631)
                     /locus_tag="DFR67_RS30555"
     CDS             complement(3005..3631)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DFR67_RS30555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158539985.1"
                     /transl_table=11
                     /translation="MTLTAILPTLRASIPDPLNTALWPATTRATLTDVVIDDVSMSYLI
                     ELSGVPAVYRSRTSAGVVVVMRVSTAGSTGHRTRAMTVDPGIDDGSPAWSELRLIGRIS
                     TARVEATTVYGPTGRPFGPMMLPADICEGDIVVAPCAMADVNDGALDRTSEMTRSGGHS
                     SGTRAMFEGSHVGRWIDDGQRECTGEPRRPDHHDQHQPAATPQRR"
     gene            3928..5004
                     /locus_tag="DFR67_RS17970"
                     /old_locus_tag="DFR67_110198"
     CDS             3928..5004
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782263.1"
                     /locus_tag="DFR67_RS17970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_110198"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_170135237.1"
                     /transl_table=11
                     /translation="MDNPTPQPILSPLTEAAIFLVATVEPGGESDTWDFLSGVTGLIRS
                     VASRAPDTRPTCVVGIGSQVWDRLFSGPRPARLAPFVPLDGSVHSAPSTPGDLLFHIRA
                     RTMDVCFELATQIATQLDGAARVIDEVHGFRYFDMRDIIGFVDGTENPDGPAAVTAVTA
                     GSEDPDFAGGSYVLVQKYLHNMPAWNALTTEEQERSIGRSKSDNIEMDEDTKPSNSHTA
                     LTVIEDATGVQVQIMRDNMPFGNVGQAEYGTYFIGYSAGPEVTDEMLRNMFLGKPEGNY
                     DRLLDFSTAVTGSRYFVPTIDFLDDPPEFRGAAAKDRGQAAPAPVDPDPEMTPLAPTMG
                     SLRIGALHRRRQLTTEEK"
     gene            5001..5807
                     /locus_tag="DFR67_RS17975"
                     /old_locus_tag="DFR67_110199"
     CDS             5001..5807
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006369314.1"
                     /locus_tag="DFR67_RS17975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_110199"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_110470859.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1.7e-93, bitscore:
                     306.5, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPISTAAWEAIEEEATRSFKRNSAARRIVDVNGPLGL
                     ELAAVTTGKRNPIAAPVAGIEASSRTSQSIVEFKVPFTVSRDAVDNVDRGAQDSDWDPV
                     IDAARTLAQAEDRAVFEGYQAAGITGIRSESPGDPIKLPDDVREYPEAISQALSRLRLS
                     SVAGPYSLALSAEVWLKVNETSNHGYPIIQHLQRVIDGDIIWAPAIDSAFLLSTRGGDF
                     DLTIGQDVSIGYDSHDSSTVNLYLQETFTFLVYTGEAAVSFDTTTG"
     gene            complement(5890..6543)
                     /locus_tag="DFR67_RS17980"
                     /old_locus_tag="DFR67_110200"
     CDS             complement(5890..6543)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020105562.1"
                     /locus_tag="DFR67_RS17980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_110200"
                     /product="HNH endonuclease family protein"
                     /protein_id="WP_110470860.1"
                     /transl_table=11
                     /translation="MVFAVTATLAAGTGVLSHRGGGPSADERRMLGELSLVPVLAQRPD
                     ATDYERSAFGQAWTDNTDAAGANNGCDTRNDILNRDLGDKVLAVVDSCARAVAAGELRS
                     PYSGHWIVFKRGKGSGAKVQIDHIVPLAYAWAMGARTWSGAMRQRFANDPANLVAVDGE
                     ANQDKSDSAPSRWMPANSGFHCQYAVQFVMVLRTYGLYLDRPSVPVLTRALEQC"
     gene            complement(6581..8914)
                     /gene="recG"
                     /locus_tag="DFR67_RS17985"
                     /old_locus_tag="DFR67_110201"
     CDS             complement(6581..8914)
                     /EC_number="3.6.4.12"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recG"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006359358.1"
                     /locus_tag="DFR67_RS17985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_110201"
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="WP_281268347.1"
                     /transl_table=11
                     /translation="MVSQEGRAIAPSVTLSDSLESVLGPKAAEMLDEQIHVQTVGELLR
                     YVPDRYVKQGDLSGERKPEPGERLTVVARVEDNKTIPIRNSRKKMVKLVVFDGKFRIAV
                     TFFNSPWIAAKLPKGSRVMMAGEVKYFRDELQLTHPNWLKLPEDGTSIEADDAVGSVLM
                     QGIAKALATDAGDGDGSDGTLDFSDFLRDVIPVYRGTAKLQAWTIWQCVRAVLGELAPI
                     EDPLPEDERAIRGLIGSDEALRKIHVPEGQHDIDQASARLRFDEALALQLALAQRRHVD
                     RGATGPSCPHVPGGLEDKLLDRLPFTLTDGQRVVIDEITADLQAPHGADRTAVVSPMSR
                     LLQGEVGSGKTLVALLAMLRVIDNGYQCALLAPTEVLAAQHFRTLETMLGGLAKEGELE
                     GEEGATRVALITGSMKTAAKRTALLETVTGQVGILIGTHALLQDNVDFFNLGFVVVDEQ
                     HRFGVEQRDTLRSRGRDGVVPHLLVMTATPIPRTVAMTVFGDLETSTLRELPRGRQPIT
                     TSVVPRGNPKWVQRVWQRVGEEVEAGRQVYVVCARIGDDGTPAGQRSQQKSSEGAAEPA
                     TAAVDMFKTLGEGPLGGHRIELLHGRLSPDEKSEVMGEFTAGHVDILVSTTVIEVGVDV
                     PNATTMVIVDADRFGVSQLHQLRGRVGRGGLPGLCLLITGSSEMSPSMERLQAVAASND
                     GFELSYVDLRQRREGDLLGSLQSGQTSSLRFLSLLTDGDLIVDTRALADEVVGSDLTLV
                     EHKPMASLIDSILGPVRLAFLDKS"
     misc_feature    complement(8866..8878)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.13"
                     /tool="antismash"
     gene            complement(8928..10580)
                     /locus_tag="DFR67_RS17990"
                     /old_locus_tag="DFR67_110202"
     CDS             complement(8928..10580)
                     /GO_function="GO:0004371 - glycerone kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006370597.1"
                     /locus_tag="DFR67_RS17990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DFR67_110202"
                     /product="DAK2 domain-containing protein"
                     /protein_id="WP_110470944.1"
                     /transl_table=11
                     /translation="MDPGLLRDWIHGTVTSLDGVRAEINDLNVFPIPDSDTGSNMLHTM
                     MAAADAVDQLDPDADTAQVARAMADGAVSKARGNSGIILSQVLVGLADAAEVNQMDGRV
                     YFNRLWVAGLRLGSLAATRAVSEPREGTVLTLLRRAGENAAQHTDATPADLARAIADGC
                     ADDLDLTQGQLDVLAQAGVVDAGGRGLLVLFDELVRVLTGVSARRRRYQGALAGGGNRV
                     GHEIGEPCDSDDEMDFEVMYLLEGARAEQISTLRANLDDLGDAVVIVGDSADAVAGEKF
                     SVHVHTCEPGLVVEAGLALGTVSDIRISCFLLDAHRGQEEKAGHPPRRKRAVVAVVTGD
                     EAAELFEQEGAVVVRADSGGAGELDADALAKAIIEVDSAHVILMGNGALPAQDLVAVAA
                     AARSRERSVVILPGGSMVQSLSAMAVHDPSQLPDDDAYAMAEAAAATRFGAVVTATERT
                     LTFAGTCEPGDQLGLIGDGVLVIDPEILGAAAGLIDLLLSTGGEMVSVLAGRNLEPRIL
                     EQLEEHVNTNHPGVEFVSFSGGQVGELLQVGVE"
     misc_feature    complement(8946..8948)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(10416..10418)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 cagatcgggg cggtgtttga ggaccacccc gagcgtgcgg gcgatgacgc cctcgtcgat
       61 ggtcgacccg ggcttttctc cgacccccag cgcaaggagc gtgtgcgccc agtcgatggt
      121 ctcggcgacc gatggcttct tccggatgtc gagcgtgcgc agtaccccga tgatccgaac
      181 cagttccttg gccaccgact cgtcgagttc ggggacccgg ctggcgagga tgtcgcgttc
      241 gcgcgccgca tccgggaagt cgatgtagag gtagaggcag cggcgtttga gtgcctcgga
      301 gagctcgcgc gtcgcgttgg acgtcaacag cacgatgggg cggcggtcag cgaccacggt
      361 gcccatctcg gggatcgtga tcgcgaagtc gctgagtacc tcgaggagca gaccttcgat
      421 gtcgacgtct gccttgtcga cctcgtcgat gagcagcacc gtcggaccgg gctgcgagat
      481 cgctttcagc agtggcctcg ggagcaagaa ctcgtccgag aagatgtcgg ccttggtctc
      541 atcccaacct cgctggccgg tcgcctggat ggcgaggatc tgcttggcgt ggttccactc
      601 gtagagcgcg cgcgcctcat cgatgccctc gtagcactgg aggcggacca gatccgcgtt
      661 ggtggactgc gagatcgccc gggccagctc ggttttgccg acaccggcag gtccctcgac
      721 gagcagcggc ttgccgagac ggtccgccaa gaagaccgag gtcgacgtag cctcgtcggc
      781 caggtaacca accccggcaa gggtctcgcg tacgccgggc acgtcggtga aactaggctg
      841 tacgcccatt ggttctccta gagattgaaa gattgaccct ggacttcgtc ggtcacaccg
      901 gccgggtctg cccgttgccc cacgcgatcc atttggtcga tgtcagctct ggcagaccca
      961 tggggccgcg agcatgcaac ttctgggtgg agataccgat ctcggcgccg aacccgaact
     1021 gctccccgtc ggtgaacgcg gtggaggcgt tgaccatgac cgccgccgcg tcgacgcgat
     1081 tggtgaactc tgtcgctgca gccaggtcat cggtgatgat cgcctcggtg tggctcgtcc
     1141 cgtaacggtc gatgtggtcg accgcctcgt cgagcccacc gacgatcttc accgcgatgt
     1201 cgaggctcag gtattcgtcg gaccagtcct cctcggtggc cgggaccatg ccgggctcgt
     1261 cgccgtggat gatcactccg gcgctctgca gcacctgggt cagccgcggc agcgccgtct
     1321 cggcgatcgc cttgtcgacg agcaccgttt cagcggtgtt gcagacgctg ggccgacggg
     1381 tcttggagtt gatcaccaga tcctccgcca tgtcgaggtc ggcagcggcg tggatgtaga
     1441 tgtggcagtt acccgtaccg gtctcgatgg tgggcacggt cgcgttctcg acgaccgcct
     1501 tgatcagccc tgcgccaccg cgcgggatga cgacgtcgac caggccgcgg gcacggatga
     1561 gatgggtgac actcgagcga tcctcggacg gtagcaactg caccgcgtca gcgggaagtc
     1621 ctgcggcggt gagggtatct cggaggatcc ggaccagctc ggcattggaa ctcgcggccg
     1681 acgacgatcc ccgcagcagc gccgagttgc ctgacttcag tgcgataccg aaagcgtcga
     1741 cggtgacgtt ggggcgggcc tcgtagacca tgcccacgac gccgagcggc acccggacct
     1801 gccgcaaggc catgccgttc ggcagcgtct tgccctggat cacgtcaccg atcggatcag
     1861 gtaacgccgc aacctgacga agtcctgccg cgatgccctc gacccgatca gcggtcagtc
     1921 gcaggcggtc gatcaggctc tcctcggtgc cggccagctc agcacgcgcg acatcttcgg
     1981 cgttcgcagc gaggatcgag ccggtctgcg cgagcagggc gtccgcggcg tcgtgcaaga
     2041 cacgattctt ccggtccgtg gtcagacctg ccaggacgcg agaggcagct ttcgacgcgg
     2101 tggcctgcgc gcgcacgatc tgttcgacat cggcgggagt ggtggtggga gcactcatcg
     2161 tcccaggata ggcggtgctg acatcacggg tctcagcgac cgacagtcag gtgtcgtggg
     2221 cgtccgcagt gagttcgcgc cacctgcgta cgttcgcgcc ggctgcaact gtcgcgtccg
     2281 cgtgcaggtg gcgcgaactt cttgatcgac ggactacgcc tcaaacgacc cgtgccgccc
     2341 cgctccgcct gcaaagcgtg tcgctccggc aacggtgtcg gcgctgaggg acgtcatccc
     2401 acgccggaac tcattgtcca tggctccggt ttcgtcgagt ccctcctgct cgagaacaga
     2461 caaccgatcc tggcgcatgc acgtctgcgg aaaggctgcg atttcgcgcg ccaattcctt
     2521 tgccgatttc agcgcatccc cctgcgagac aatacgattg accagtccca tccgatgcgc
     2581 ttcattcgcc cgtacctccc ggcccgtcaa gatcatgtcc atcgcgtgcg acgtcccgac
     2641 caggcgcggc aaccgcactg tgccaccgtc gatcaacgga acgccccacc gacgacagaa
     2701 gacgccgaag accgcatctt cttcggcgat ccgcagatcc gcccagagtg ccaactcgag
     2761 accacccgcg accgcgtgac cggcgatcgc ggcaatgacc ggtttcgaca accgcattcg
     2821 agtcggaccc atcggtccgt cgcccgccga atcgatccgg ttgggcgcct ccccggcggc
     2881 aatggccttc agatcagctc ctgcgcagaa ggttccacct tcgccgtgca gaactgcgac
     2941 cgccgcacca tcgtcaccct cgaagtcgcg aaatgccgcg gccaaggcct ccgcagtcgg
     3001 accatcaacg gcgttgcggc gttgcggccg gttgatgctg atcgtggtga tcgggccgtc
     3061 gtggctcacc agtacattcg cgctggccgt catctatcca ccttccgaca tgagacccct
     3121 cgaacatagc cctggtgccg ctgctgtgac cgccagatcg cgtcatctcg gaagtgcgat
     3181 cgagagcgcc atcgttgacg tcggccatcg cacaaggcgc cacgacgata tctccctcgc
     3241 agatgtcagc gggcagcatc atcggaccga acggccgacc ggtagggccg tacaccgttg
     3301 tcgcctcgac ccgcgcggtc gagatccgac cgatcaacct gagttcggac cacgccggag
     3361 agccgtcatc aattcctggg tcgacagtca tcgcgcgggt tcggtggccg gtagaccctg
     3421 cagtcgacac cctcatgacc acgacgacgc cggccgacgt tcggctccgg tacaccgcag
     3481 gaacgccaga caactcgatc aggtagctca tcgacacatc gtcgatgacg acgtcggtca
     3541 aggttgcccg agtggtcgcc ggccacagcg ccgtgttcaa cggatcggga atgctcgcgc
     3601 gcagggtcgg caagatcgcg gtcagggtca ccgccgtcca cccctcacgt tgtgttcgtc
     3661 ttcttcgtcg cgcactcgcg agcattcacc gttgatcacc actccgttct acggttcagg
     3721 tcacgggatc gacaggtcct ggcgagagtt ttgacgagat ctttgcggct gtgtcccagc
     3781 ggacttcggt tcgaatgatc cgtaggtggg acgcatcgat atgcagtact cgacatcaac
     3841 cgcgagcggt gcgggcggca tggcctcgca gaagttatgg ttgtcacata cgtccagcag
     3901 caggcggaag gtcagctcat ctcacccatt gacaacccca ccccgcagcc gatcctgtca
     3961 ccgctgacgg aggccgcgat cttcctggtg gcgacggtcg aacccggtgg ggaatccgac
     4021 acatgggact ttctctcagg tgtcaccgga ctcattcgtt cagtggcctc tcgcgccccc
     4081 gacacccggc ccacctgtgt cgtcgggatc ggatcgcagg tatgggaccg actcttcagt
     4141 ggcccacgcc ccgcccgtct agcaccgttc gtgcccctcg acggctccgt tcacagtgcc
     4201 ccgtcgaccc ccggcgatct gctgttccac atccgtgccc gcaccatgga tgtctgcttc
     4261 gagttggcca cccagatcgc cacacaactc gacggtgccg cccgcgtcat cgacgaggtc
     4321 cacggtttcc gctacttcga catgcgcgac atcatcggtt tcgtcgacgg caccgagaat
     4381 cccgacggac cggcggctgt tacggcggtc accgcaggca gcgaggatcc cgatttcgcg
     4441 ggcggcagtt acgtcttggt gcagaagtac ctgcacaaca tgcccgcctg gaacgcgctc
     4501 acgaccgagg agcaggaacg gtccatcggc cggtcgaagt cggacaacat tgagatggac
     4561 gaggatacaa agccttccaa ttcgcacacg gcgctgaccg tcatcgaaga cgccacgggc
     4621 gtgcaggtgc agatcatgcg ggacaacatg ccgttcggca acgtcgggca ggccgaatac
     4681 ggcacctact tcatcgggta ctccgccgga cccgaggtga ccgacgagat gctgcgaaac
     4741 atgttcctgg gcaaacccga gggcaactac gacaggctcc tcgacttctc caccgcagtc
     4801 acgggcagcc gatacttcgt gcccacgatc gactttctcg acgacccacc tgagttccgc
     4861 ggcgcagccg ccaaagaccg cggccaagcc gcacctgccc cggtcgaccc cgacccggag
     4921 atgacaccac tcgcacccac catgggttcg ttgcgtatcg gcgccctgca ccggcgacgc
     4981 cagctgacga cggaggaaaa atgaacaacc tgcatcgcga actagcgccc atctcgacgg
     5041 cggcctggga ggccatcgaa gaggaggcga cccgatcgtt caagcgcaac agcgccgctc
     5101 gtcgcatcgt ggacgtgaac ggcccgctcg gactagaact cgccgcggtg accaccggca
     5161 agcgcaaccc catcgcggcg ccggtcgcgg gcatcgaggc cagtagccgc acctcgcagt
     5221 ccatcgtcga gttcaaggtg cccttcaccg tcagccgaga cgccgtcgac aacgtcgatc
     5281 gcggtgcgca ggattccgac tgggacccgg tgatcgacgc ggcccggaca ctcgcacaag
     5341 ccgaggaccg cgctgtgttc gagggctacc aggcggcggg catcacgggt atccgcagcg
     5401 aatcgccggg cgatcccatc aaactccccg acgacgtacg cgagtacccc gaagcgatca
     5461 gccaggcgct gagccgactt cgcctgtcgt cggtggcagg cccgtattct ctcgcgctca
     5521 gcgccgaggt ctggctcaag gtgaacgaga cgtccaacca cggatatccg atcatccagc
     5581 acctacagcg cgtgatcgac ggagacatca tctgggcccc ggccatcgac tcggccttcc
     5641 tgctcagcac ccgtggcgga gacttcgatc tcaccatcgg acaggacgtg tcgatcggtt
     5701 acgactccca tgactcctcg acggtcaacc tgtacctgca ggagacgttc acgttcctcg
     5761 tctacaccgg cgaagcggca gtgtcgttcg acacgacgac aggctgaccc gtagaaacag
     5821 gcgaattcgg aagacccaga tgttgcagca gctgggtctt cctgattcga ctgggtttgt
     5881 ttttcaggct caacactgtt cgagggcacg ggtgagcacg ggcacggaag gccgatcgag
     5941 atacagaccg tacgtccgca gcaccatgac gaactgaacc gcgtactgac agtggaatcc
     6001 tgagttcgcc ggcatccagc gcgagggtgc ggagtcactc ttgtcctggt tcgcctcgcc
     6061 gtccaccgcc accaggttgg cggggtcatt cgcgaaacgc tgccgcatcg caccactcca
     6121 cgtacgtgcg cccatggccc acgcgtaggc gagcggcacg atgtggtcga tctggacctt
     6181 ggcgccagac cccttgccgc gcttgaacac gatccagtga ccactgtagg gactgcgaag
     6241 ctcacccgcg gcgaccgcac gcgcgcaact gtcgaccacc gccaggacct tgtcgcccag
     6301 atcgcgattc aggatgtcgt tgcgggtgtc gcacccgttg ttcgccccgg cggcatcggt
     6361 gttgtcggtc cacgcctgcc cgaaagcgct ccgctcgtag tcggtggcgt ccggacgttg
     6421 ggccagcacc ggcacgaggg acagttcccc gagcattcgc cgctcgtccg ccgacggtcc
     6481 tcctccgcgg tgcgacagca cgccggtacc ggcggcgagt gtcgcagtga ccgcgaacac
     6541 catcagtagc acccaccagc gagacgagag acctacgcca tcaggacttg tcgagaaacg
     6601 ccaggcgtac cggacccagg atcgagtcga tgagggacgc catgggtttg tgttcgacga
     6661 gcgtgaggtc gctgcccacc acctcgtcgg cgagcgcacg cgtgtcgacg atgagatcgc
     6721 cgtcggtgag gagcgagagg aaccggagcg atgaggtctg cccggactgg agcgagccaa
     6781 gcagatcacc ttcccttcgc tgacggaggt cgacgtacga caactcgaac ccatcattgg
     6841 acgcggcgac cgcctgcagc cgttccatcg acggcgacat ctccgacgat ccggtgatga
     6901 gcaggcacaa tccgggcaaa ccaccacgcc ctacacgacc ccgaagctgg tggagctggc
     6961 tgaccccgaa tcggtcggca tcgacgatca ccatggtggt ggcattcggg acgtcgacgc
     7021 ccacctcgat gaccgtggtg gagacgagga tgtcgacgtg tccggcggtg aactccccca
     7081 tcacctcgct tttctcgtcg ggcgacaacc tgccgtgcag caactcgatg cggtgcccgc
     7141 cgagcggtcc ctcaccgagg gtcttgaaca tgtccaccgc cgcagtcgct ggttcggccg
     7201 caccctccga cgacttctgc tgcgaccgct ggcccgcagg cgtcccgtcg tcgccgatgc
     7261 gtgcgcacac cacatagact tgccggccgg cctcgacctc ttcgccgacg cgctgccaga
     7321 cccgttgtac ccatttggga ttgccgcggg gcaccacact cgtggtgatc ggctgccggc
     7381 cccgaggtag ttcccggaga gtcgaggtct ccaggtcccc gaagaccgtc atggcgaccg
     7441 tccgagggat gggggttgcg gtcatcacca acaggtgagg caccacacca tcgcggccgc
     7501 gagaccgcaa ggtgtcgcgt tgctccaccc cgaagcggtg ctgttcgtcg acaacgacaa
     7561 aacccagatt gaagaagtcg acgttgtctt gcagcagggc gtgggttccg atgaggatgc
     7621 ccacctggcc ggtgaccgtc tcgagcaagg cggttcgttt ggctgccgtc ttcatcgatc
     7681 cggtgatcag agcgactcgt gtcgccccct cttctccttc gagttcgcct tccttggcga
     7741 gcccacccag catcgtctcc agagttctga aatgctgcgc cgcaagcact tccgtggggg
     7801 ccaacagggc gcactgatag ccgttgtcga tcactcggag catcgccagc aacgcgacca
     7861 aggtcttccc cgagccgacc tcaccctgca ggagcctgct catcggcgac accacagcgg
     7921 tccggtccgc gccgtgcgga gcctgcaggt cggcggtgat ctcatcgatc accacgcgtt
     7981 ggccatcggt gagggtgaac ggcaaacggt ccagcagctt gtcctcgagt ccgcccggga
     8041 cgtgcgggca cgacggccct gtcgctcccc gatccacatg acggcgttgc gcgagcgcga
     8101 gttggagcgc cagcgcttca tcgaacctga gacgggcact ggcctggtcg atgtcgtgtt
     8161 gaccctccgg aacatggatc ttgcgcaacg cctcatccga gccgatcaaa cctcggatgg
     8221 cccgttcatc ttctggaagc ggatcctcga tcggcgcgag ctcgccgaga accgcccgca
     8281 cgcactgcca gatggtccag gcctgcagct tggccgtccc gcggtagacc ggaatcacat
     8341 cacgcaggaa gtcggagaaa tcgagggttc cgtccgagcc gtcaccgtca ccggcgtcgg
     8401 tcgcaagggc tttggcaatg ccctgcatca gcaccgaccc gactgcgtcg tcggcttcga
     8461 tcgacgtgcc gtcctccggc agtttgagcc agttcgggtg ggtcaattgc agttcgtcgc
     8521 gaaagtactt gacctcgccc gccatcatga cccgcgatcc cttcggcagc ttggccgcga
     8581 tccacgggga gttgaagaaa gtcacggcga tgcggaactt cccgtcgaag acgacgagct
     8641 tgaccatctt cttccgcgag ttgcgaatcg ggatcgtctt gttgtcctcg acccgcgcaa
     8701 ccaccgtcag ccgttccccg ggttcgggtt tgcgctcccc tgacaggtct ccctgtttga
     8761 catatcgatc aggcacgtac cggagcaact caccgacggt ctgtacgtga atctgctcat
     8821 ccagcatctc ggcagccttg ggaccgagca ccgactccaa cgaatctgac agcgtcaccg
     8881 acggcgcgat ggcgcgaccc tcttgtgaaa ccactactgc gtcaccacta ttcgacaccc
     8941 acctgtaaca actcgcctac ctgtcccccg gaaaagctca cgaactcgac acccgggtgg
     9001 ttagtgttca cgtgctcctc gagctgttcg aggatgcgcg gctcgagatt gcgaccggcc
     9061 agcacgctga ccatctcacc gccggtactg agcagtaggt cgatcagacc ggctgcagca
     9121 ccaaggattt cgggatcaat gacgagcacg ccgtcaccga tgaggccgag ctggtcgccg
     9181 ggctcgcagg tgcccgcgaa ggtcagtgtc ctctcggttg ccgtgaccac cgcaccgaac
     9241 ctggtggccg cggcggcttc tgccatcgcg taggcgtcgt cgtccgggag ttgactcgga
     9301 tcgtgcacag ccatcgccga caggctctgc accatcgaac ccccaggcag gatgaccacc
     9361 gagcgctcac gcgatctggc cgccgcggcg acggcgacca ggtcctgggc cggcagtgca
     9421 ccgtttccca tgaggatcac gtgcgcgctg tcgacctcga tgatggcttt ggcgagcgcg
     9481 tccgcgtcca gttcgccggc tccgcccgag tccgcccgga ccaccaccgc gccctcctgc
     9541 tcgaacagct cagccgcctc gtcgccggtc accactgcca ccaccgcacg tttacggcgc
     9601 ggcggatggc ccgccttttc ttcctggccg cggtgggcgt cgaggagaaa gcaactgatc
     9661 cggatgtcgc tcaccgttcc caacgcgagt cccgcttcga cgacgaggcc gggttcgcac
     9721 gtgtggacgt ggacggagaa cttctctccg gccacggcgt cggcgctgtc accgacgatg
     9781 accaccgcgt cacccaggtc gtcgaggttc gctcggaggg tcgagatctg ttcagctcga
     9841 gcaccttcga gtagatacat cacctcgaaa tccatctcgt catcgctgtc gcacggctca
     9901 ccgatctcgt gccccacccg gtttccccca ccggcgagcg cgccttgata ccggcgtcgg
     9961 cgcgcggaga cgccggtgag aacccgaacc aactcgtcga acagcaccaa cagcccgcgg
    10021 ccgccggcat cgaccacacc cgcctgcgcg agcacatcga gttgcccctg cgtcaggtcc
    10081 aggtcatcgg cgcaaccgtc tgcgatcgca cgggccaggt cggccggagt ggcatcggtg
    10141 tgctgcgctg cgttctcacc tgcgcggcgc agcaaggtca gcacggtgcc ctcccggggc
    10201 tcactgaccg cacgggtcgc cgccagcgat cccaaccgca gtccggcgac ccagagccgg
    10261 ttgaagtaga cccgtccgtc catctggttg acctcggcgg cgtcggccag tcccacgagc
    10321 acctgcgaca ggatgatccc cgaattaccc cgcgccttcg agacagcccc gtcggccatc
    10381 gctcgcgcca cctgcgcggt gtcggcgtcg ggatctaatt gatccacggc atcagccgcc
    10441 gccatcatcg tgtgcaacat attgctgccc gtgtcggagt ccgggatcgg gaacacgttg
    10501 agatcgttga tctcggcccg cacaccatcc aggctcgtca cggtgccgtg gatccaatcg
    10561 cgcagcagac ccgggtccat cgcgggagcc ccaacctgct ctgtcactgc ccgcacctcc
    10621 tcggtctgac gcacagccta atctgttgcc ggccgagctc ctgcgacgtc gctccgtcac
    10681 agtgcttcag gccggagcct gagctccacc attttacgga gacgaagcga agtgaggtca
    10741 ggccggtttt gatcatcgcc ggtgagccgc tattcttgca gggttgccta tcggacaccg
    10801 gtgcatg
//
