LOCUS       NZ_CP029541            10732 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. NEAU-S7GS2 chromosome, complete genome.
ACCESSION   NZ_CP029541
VERSION     NZ_CP029541.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NEAU-S7GS2
  ORGANISM  Streptomyces sp. NEAU-S7GS2
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Streptomyces sp. NEAU-S7GS2 isolated from saline-alkaline soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) School of Life Science, Northeast
            Agricultural University, Mucai Street, Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: AMRT Link v. v5.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003173275.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 05:28:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,458
            CDSs (total)                      :: 8,365
            Genes (coding)                    :: 8,052
            CDSs (with protein)               :: 8,052
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 313
            CDSs (without protein)            :: 313
            Pseudo Genes (ambiguous residues) :: 0 of 313
            Pseudo Genes (frameshifted)       :: 144 of 313
            Pseudo Genes (incomplete)         :: 230 of 313
            Pseudo Genes (internal stop)      :: 21 of 313
            Pseudo Genes (multiple problems)  :: 74 of 313
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:52:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9565230
            Orig. end                         :: 9575962
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10732
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="38"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10732
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10732
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5732](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5732)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(509..1495)
                     /locus_tag="DKG71_RS41830"
                     /old_locus_tag="DKG71_41830"
     CDS             complement(509..1495)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003963309.1"
                     /locus_tag="DKG71_RS41830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41830"
                     /product="hypothetical protein"
                     /protein_id="WP_018093175.1"
                     /transl_table=11
                     /translation="MTPSSVTGPASRPRPADEPRSVARSALRLRAFFAERAPTRIYLTY
                     AVLWVLALQGTLAVLAPESDWRLGGSLLTEIATVYLALLFIRVVDEQKDLEYDRQYNPD
                     RPLPRGAIDVVELRAAMVLIVVAELALTGWRSPLLAGWLLLDLGYICFLVQLERWSARV
                     RDRIFVNLLVSYPVQALLSVHIWLSFRQATGTGPVPDAVFGALLCACVFLHYEFARKTG
                     ARVVPGASLYSNVVGLRGSVALTSGFALGAVGLALALVRPWELHGAVALAGWLPLSALG
                     FVWGGGERFASLRTPTWPPGPAMGFLAWCYLGVFITALVVARPGFGG"
     gene            complement(1492..4158)
                     /locus_tag="DKG71_RS41835"
                     /old_locus_tag="DKG71_41835"
     CDS             complement(1492..4158)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PPDK_N"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093174.1"
                     /locus_tag="DKG71_RS41835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41835"
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="WP_239472320.1"
                     /sec_met_domain="PPDK_N (E-value: 2.3e-55, bitscore: 187.1,
                     seeds: 105, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRGRALLNEAEVGPKFARQARMAAAGLPVPRFFCLSAAVFTQVL
                     SPMRAEVDAVLGDVDAGDSEALGKKASILREMVLRAPVPAAAERQLYEAFDQEFGPGAV
                     VSVRSSVVGSGAQAGEDSAHDAFAGISDSFLYVTRDQLLDKVRQCWASAFGPQALLYRA
                     ARGGTQHGFEVAVGVQRMEFGRRSFVLFTCDPLTGARDRVLAAGLGIGEGVVQEKVPVD
                     HYFISRRDDAVRSVLAHKTQMMEENPERLADGPRTAPVPEELCDTAVLTEEQIRKVVAT
                     GDRIERLFGCPQDIEGTFTADGTLHILQARPVVLELDRQRLWSNANITESYPDVTTALT
                     YSFAQRFYREDFHDFYRRLGVRRTVLDRHEGELRGMIGLLRGRVYYSLSVWYTLHGLSR
                     TFPLWRGSFERMMGMSPSVTHVRPTPPLTCPGQLPATSAALVRLAVSLLTHGAAARNFE
                     AWWQRTLAEHRTAATTADPLQLVQLMRSLWREAGSRWGITLVNDILLQMHETLAMRLFE
                     RWLPQADPGLHSDLLCGGEENRSVVILMSVIDIAERVRGIPAVLDALAVKTAEEVWEEV
                     DDGAYGADLADRLRTHVELHGDRGLQELKLEVLLPRDQPSQLLRTAAEYARGDLTRQLL
                     RTREAEARRAGENRLAGLLTGHPLRRRVLRAVVAAQRRHINIRENTRYNRSELFGFARH
                     VYHRLGADLAARGLLESAQDVVHLTEEEILGTYDGTAVTDDLRGLAALRRAEYHARGAE
                     LPMDFVTMGPVRDGLPDPNRGTAAEGTLHGLGSSGGVVRGTARVVVDPHQLGEPTDDMI
                     LVARETDPGWLFLMLSARGIVVERGTLLSHTAISGRKFGIPTIVALQHATTRIPDGARV
                     EMDGAAGTVTILEEDEA"
     misc_feature    complement(3040..3042)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4227..4871)
                     /locus_tag="DKG71_RS41840"
                     /old_locus_tag="DKG71_41840"
     CDS             complement(4227..4871)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1150: isoprenylcysteine carboxyl methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003953130.1"
                     /locus_tag="DKG71_RS41840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41840"
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="WP_109895654.1"
                     /transl_table=11
                     /translation="MVWVLRKFPLFVLAVCLAATATALVRGALDFSDPWQRVAMLLAGL
                     HLLWLLLETWTTVRSSTSTETATDRGTIYVYAAARAATVAAACLPSADQQTYHPWMLAV
                     PVVFAAAVGVRLSAIRTLGRFYSHRVRVLSDHQIVRTGPYRWIRHPAYFGMALAHIAFV
                     VFFLNTYSAAALMILLPTLVVRILVEERTLLTLPGYSEYAATHRRVIPWIW"
     gene            complement(5001..5732)
                     /locus_tag="DKG71_RS41845"
                     /old_locus_tag="DKG71_41845"
     CDS             complement(5001..5732)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093172.1"
                     /locus_tag="DKG71_RS41845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41845"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_109895656.1"
                     /sec_met_domain="AfsA (E-value: 7e-13, bitscore: 47.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEKVVFVVGDNLATRSNSVDLVSLSEFTASLKGELISEPFPVII
                     PGQGIEDYEREIVRSKLRQHGLPESILKDIPLPELLYHSEVHKRKPENVLIAGLRKVED
                     DLFKATLRISDRQEMVLDHTTGSHVTAMVITEAVRQISLAVGERYLLAPSGRARRFILN
                     SLQTTFHKFLLPLPAQLEYSVQEVSQKGPNRLRFRGRCDLVQAGVVAASGSMDMVVMEE
                     ERADRIEAERIRETTRALAEL"
     misc_feature    complement(5532..5534)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(5792..5812)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.23"
                     /tool="antismash"
     gene            6154..>6447
                     /locus_tag="DKG71_RS45375"
                     /pseudo=""
     CDS             6154..>6447
                     /GO_function="GO:0009039 - urease activity [Evidence IEA]"
                     /GO_function="GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /GO_process="GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608038.1"
                     /locus_tag="DKG71_RS45375"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="urease subunit gamma"
                     /pseudo=""
                     /transl_table=11
                     /translation="MHLTPHEQERLLIHVAADVAQRRRGSGLLLNYPEVMALLTVHVFE
                     QARAGKTVSDIMDSGRQLLSRGEVMDGVPEMIKNVQVEATFPDGTKLVTIHDP"
     gene            <6655..6963
                     /locus_tag="DKG71_RS45380"
                     /old_locus_tag="DKG71_41855"
                     /pseudo=""
     CDS             <6655..6963
                     /GO_component="GO:0035550 - urease complex [Evidence IEA]"
                     /GO_process="GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012902.4"
                     /locus_tag="DKG71_RS45380"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41855"
                     /product="urease subunit beta"
                     /pseudo=""
                     /transl_table=11
                     /translation="LPGEGRTRIRVKNMSDRPIQVGSHYHFAEVNPGLKVLKVEVPSGP
                     ELEPPQELKDCEAARMRRLNIAAGTSVRFEPGDECCVELVEIQGDRQVKGLREETYQ"
     misc_feature    complement(6685..6700)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.21"
                     /tool="antismash"
     gene            7032..9011
                     /locus_tag="DKG71_RS41860"
                     /old_locus_tag="DKG71_41860"
     CDS             7032..9011
                     /EC_number="3.5.1.5"
                     /GO_function="GO:0009039 - urease activity [Evidence IEA]"
                     /GO_process="GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607071.1"
                     /locus_tag="DKG71_RS41860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41860"
                     /product="urease subunit alpha"
                     /protein_id="WP_109896823.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.1e-56, bitscore:
                     191.5, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKRAEYATLYGPTTKDRVRLADTALTVEVEADWSGGPKHSGNEM
                     IFGGGKVIRESMGMSHIPRDGDAEGRKPVDTVITGALILDWWGVVKADVGIRGGEIAAI
                     GKAYNPETMDPIPDDEFETPDRTSPSDKLPVQQTLPVQPTNFVVGPSTEVISGNGRILT
                     AGGVDTHVHFICPEQIHEALASGVTTLIGGGTGPAEGSTATTVTPGEWHITRVFGSLDA
                     YPVNIGLLGKGSTVNTDALNEQVDAGVIGFKVHEDWGATPAVINAALDVCESRQVQLAL
                     HADSLNESGFLDSTRNAFTKDGKKRPVHIFHVEGAGGGHAPDMIELVKEENVLPASTNP
                     TRPLTVNTVKEHVDMMVVCHHLNPDIPADMAFADSRIRPSTMAAEDLLHDMGAISMMSS
                     DAQAMGRIGEMIMRTWQTAHVMKTRYGPLKEDLENAKVRPIADDTDHSHNADRLLPNDN
                     YRARRYVAKYTINPAITHGIDGHVGSVETGKLADLVLWEPKFFGVKMHMVLKGGQLAYA
                     QVGDANASITTPQPFLPRAVWGSTGRSPLSNSYNFVAPGVADTLNSRVVTVQDSSGNVT
                     EMKIGGLGLAKKFVDISTSIRDITKADMKLNDTVPESLKVDHNSFEVTIGGATTGDART
                     ELNGVTVPRTYVSEVPMAQRYFLF"
     gene            9125..9799
                     /locus_tag="DKG71_RS41865"
                     /old_locus_tag="DKG71_41865"
     CDS             9125..9799
                     /GO_function="GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /GO_process="GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543361.1"
                     /locus_tag="DKG71_RS41865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41865"
                     /product="urease accessory UreF family protein"
                     /protein_id="WP_109895658.1"
                     /transl_table=11
                     /translation="MRRAALLLLADGRFPAGGHAHSGGVEAAVAHKAVHDVDSLEAFCR
                     GRLHTTGLTTAGLAAAAAVGCDPLLLDDAADARTPAPALRAVARRLGRQMMRAARATFP
                     SAELDRLAAERPQGAHQPIVQGLAAQAAGLTPQDAACAAAYESTGGPATAAVRLLSLDP
                     LDASRLLARLSAEIDTVAASAADAAARVVTEGLDALPSASSPLLDITGEQHAAWTVRLF
                     AS"
     gene            9826..10521
                     /gene="ureG"
                     /locus_tag="DKG71_RS41870"
                     /old_locus_tag="DKG71_41870"
     CDS             9826..10521
                     /GO_function="GO:0016530 - metallochaperone activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ureG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093168.1"
                     /locus_tag="DKG71_RS41870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41870"
                     /product="urease accessory protein UreG"
                     /protein_id="WP_109895660.1"
                     /transl_table=11
                     /translation="MHLDHPVTMPHRHTYSAEPQRTDGSRRAFRVGLGGPVGSGKTASV
                     AALCRTLRDELSLAVVTNDIYTREDAEFLLREAVLPPERITAVETGACPHTAIRDDISA
                     NLEAVEHFDETLDPLDLVLIESGGDNLTATFSKGLVDAQIFVIDVSSGDDIPRKGGPGI
                     TTADLLVVNKTDLAPHVGADLATMAADAKRQRGDRPVVFTSLTSDDGIREIADWVTGHL
                     TRWRAKASA"
ORIGIN
        1 ccgacgaacc cctgggagag gcatgccgcg ggatcgggcc atcgggccga gtcgacccgt
       61 cgtacggcgc cggcgggcag cagcgcgtat tgctgccagc cgaagtcgtg ccggaccagc
      121 tcgccggggc gcagtccggt ccccggtgcc tcgaccacct cgccgagagc ggcgccgcgc
      181 agggcccggc ccggccggca ggagggcatc ggcagcacct cggcatccgc catcagggtg
      241 cgggtggcag caccgatccg cagcaccaga ttccgcacca gcacctggcc ggcggacggt
      301 cggcgcagtg ggacctccac cgtctcgaaa tgctccggcc gcagctcccg gcccgggtgg
      361 gcggccagcc ggacctcacg gtggacggag ggcggcgcga cggagcccgt gccgccctct
      421 gccgccccga acccgctgct gcccgggcgg ccttcgggat ccgggcggcc cgcggcatcc
      481 gctccgggtg tgcgccgcgc catcggcgtc atcccccgaa ccccggccgg gccaccacca
      541 gtgcggtgat gaacacgccg aggtagcacc aagccaggaa gcccatggcc ggaccgggcg
      601 gccaggtcgg cgtgcgcagg gaggcgaacc gttcgcctcc gccccagacg aagcccagcg
      661 cggacagcgg aagccagccc gccagcgcca cggcgccgtg cagttcccag ggacgcacca
      721 gtgcgagcgc cagccccacg gcgcccagcg cgaagcccga ggtcagcgcc accgagccgc
      781 gcaggccgac gacgttcgaa tagagcgacg caccgggcac cacgcgggca ccggtcttgc
      841 gggcgaactc gtagtgcagg aagacgcagg cgcacagcaa ggcgccgaac accgcgtccg
      901 gcaccggacc ggtgccggtg gcctgccgga aggacagcca gatgtgcacg ctgagcaacg
      961 cctgcaccgg atagctcacc agcaggttga cgaagatccg gtcccgtacc ctcgccgacc
     1021 agcgctccag ctggaccagg aagcagatgt acccgaggtc cagcagcagc catccggcca
     1081 gcagcggtga gcgccagccg gtgagcgcca gttccgcgac gacgatcagt accatggcgg
     1141 cccgcagttc caccacgtcg atcgcgccgc gcggcagcgg ccggtcaggg ttgtactgcc
     1201 ggtcgtactc caggtccttc tgctcgtcga cgacccggat gaacagcagc gccagataga
     1261 cggtggcgat ctcggtgagg agggaacccc cgagtcgcca gtccgactcc ggcgcgagca
     1321 ccgccagtgt gccctggagg gccagcaccc acagcaccgc ataggtcaag tagatccggg
     1381 tgggggcccg ttcggcgaag aaggcgcgca gacggagggc gctgcgtgcc acggagcggg
     1441 gttcgtccgc cgggcggggg cgggacgcgg ggccggtcac ggaagagggt gtcatgcctc
     1501 gtcctcctca aggatcgtca ccgtgccggc cgcgccgtcc atctcgacgc gggcgccgtc
     1561 cgggatgcgg gttgtcgcgt gctggagcgc cacgatggtg gggatgccga acttgcgtcc
     1621 agagattgcg gtgtgcgaca gcagggtgcc gcgctcgacg acgatgccgc gggcggacag
     1681 catcaggaac agccagccgg ggtcggtctc cctggctacc aggatcatgt cgtccgtggg
     1741 ttcgccgagc tgatgcgggt cgacaaccac ccgggcggtg cctcgcacga cgcccccgct
     1801 ggagcccagg ccgtgcagag tgccctcggc ggccgtgccc cggttcgggt ccggtagccc
     1861 gtcacggacg gggcccatgg tcacgaagtc catcggcagt tcggcgcccc gggcgtggta
     1921 ctcggcccgg cgcagggccg ccaggccacg caggtcgtcg gtgaccgcgg taccgtcgta
     1981 ggtgccgagg atctcctcct cggtcaggtg aaccacgtcc tgcgccgatt ccagcagacc
     2041 gcgcgcggcc aggtcagcgc cgagccggtg gtacacgtgg cgggcgaagc cgaacaactc
     2101 gctgcggttg tagcgggtgt tctcccggat gttgatgtgc cggcgctgcg cggccaccac
     2161 cgcgcgcagc acccggcggc gcagcgggtg tccggtgagc aggccggcca gtcggttttc
     2221 gccggcccgc cgggcttcgg cctcgcgggt gcgcagcagt tgtcgcgtga ggtcgccccg
     2281 ggcgtactcc gcggccgtgc gcaggagctg ggacggttgg tcgcgcggta gcagcacctc
     2341 cagcttcagc tcttgcaggc cccggtcgcc gtggagttcc acatgcgtcc gcagccggtc
     2401 ggcgagatcg gctccgtagg cgccgtcgtc gacctcctcc cacacctcct cggcggtctt
     2461 gacggcgagc gcgtcgagca ccgccggtat gccgcgaacc cgttcggcga tgtcgatgac
     2521 cgacatcagg atgacgacgc tgcggttctc ctccccgccg cacagcaggt cgctgtggag
     2581 cccggggtcg gcctggggca gccaccgctc gaacagccgc atggccagcg tctcgtgcat
     2641 ctggagcagg atgtcgttga ccaacgtgat gccccagcgg ctgcccgctt cccgccacaa
     2701 cgaccgcatc agctgcacca gttgcagcgg atcggccgtc gtggccgcgg tccggtgctc
     2761 ggcgagggtc cgctgccacc acgcctcgaa gttccgggcc gccgcgccat gcgtcagcag
     2821 gctcaccgcc agccgcacca aggccgccga ggtggcgggc agttggccgg gacaggtgag
     2881 gggcggggtg ggccggacgt gggtgaccga cggcgacatc cccatcatcc gttcgaagct
     2941 gccgcgccac agcgggaagg tcctgctgag tccgtgcagg gtgtaccaga cactcagcga
     3001 gtagtagacc ctgccgcgca acagcccgat cattcctcgt aattcgccct cgtgccggtc
     3061 gagcaccgtg cggcggacgc cgagcctgcg gtagaagtcg tgaaagtcct cgcggtagaa
     3121 gcgctgggcg aaggagtagg tcagtgcggt ggtgacgtcc gggtagctct cggtgatgtt
     3181 ggcgttgctc cacaatcgct ggcggtcaag ctccagcacc accggccggg cctgcaggat
     3241 atgcagggtt ccgtcggcgg tgaaggtccc ctcgatatcc tgcgggcagc cgaagagtct
     3301 ctcgatccgg tccccggtcg ccactacctt ccggatctgc tcttccgtca gtacggccgt
     3361 gtcgcacagt tcttcaggca cgggtgccgt ccgcggtcca tcggcgagcc gctcggggtt
     3421 ctcctccatc atctgcgtct tgtgcgcgag caccgaccgc accgcgtcgt cgcgccggct
     3481 gatgaagtag tggtcgaccg gcaccttctc ctggaccacg ccctcgccga tgccgagacc
     3541 ggcggcgaga acccggtcgc gggcaccggt cagcggatcg caggtgaaca ggacgaagga
     3601 gcggcgcccg aactccatgc gctggacgcc gacggccacc tcgaacccgt gctgcgtgcc
     3661 cccgcgggcg gcgcggtaca gcagcgcctg cgggccgaat gcggaggccc agcactggcg
     3721 taccttgtcc agcagttggt cccgcgtcac gtagaggaag ctgtcgctga tgccggcgaa
     3781 cgcgtcgtgc gccgagtcct cgccggcctg ggcaccggac ccgacgaccg acgagcgcac
     3841 cgagaccacc gcgccgggcc cgaactcctg gtcgaacgcc tcatatagct gccgttcggc
     3901 cgccgccggc accggtgccc tcagtaccat ctcccgcagg atcgaagcct ttttccccag
     3961 ggcctcggaa tccccggcgt cgacgtcgcc gagtacggcg tccacctcgg cgcgcatggg
     4021 gctcaacacc tgggtgaaca cggcggccga caggcagaag aaccggggca ccggcagacc
     4081 ggccgccgcc atccgcgcct gccgggcgaa tttcggcccg acctctgcct cattcagcag
     4141 tgcgcgtccg cgcagcaccg gccgccgatc ggcgctcggt aaaggcttcg atatgcgcag
     4201 tgaacggagt atattcatag ggcatttcac catatccagg gaatgacacg gcggtgggtc
     4261 gccgcatatt cggagtagcc gggaagggtg agcagtgttc tctcctccac gagaatgcgc
     4321 accacgagcg tcggcagcag aatcatcaag gccgccgcgc tgtaggtgtt gaggaagaag
     4381 accacgaagg cgatatgagc cagcgccatg ccgaagtacg ccggatgccg aatccaccgg
     4441 tacggtccgg tccggacgat ctggtggtcg ctgagcacac gcacccggtg cgagtagaaa
     4501 cgcccgagcg tgcggatcgc tgacagccgc acgccgaccg ccgccgcgaa gaccactggc
     4561 accgcaagca tccacgggtg atacgtctgc tggtcggccg atggcaaaca ggccgcggcg
     4621 acggtggccg cgcgtgcggc ggcgtacacg tagatcgtgc cgcggtcggt ggccgtctcg
     4681 gtcgacgtcg aactgcggac ggtcgtccag gtctccagca gcagccacag caggtggagc
     4741 ccggcgagca gcatcgccac ccgctgccac gggtcggaga agtcgagcgc cccacggacc
     4801 agcgccgttg cggtcgccgc caggcagacg gcgagcacga acagcggaaa cttgcgcaga
     4861 acccacacca taagcacctc cacaaagcag cccgcaggca acgaggggcc cgcgccgagc
     4921 tggtgtcgcc cgtcgctgac aacccgtgat caggacgcgg ccggccggct gaggaccatg
     4981 cgcgccgcat cgccctcctc ctagagttcg gccagggcgc gcgtcgtctc gcggatgcgc
     5041 tccgcctcga tccggtcggc ccgctcctcc tccatcacca ccatgtccat ggagcccgac
     5101 gcggcgacga cgcccgcctg caccaggtcg cagcggcccc ggaaccgcag ccggttcggc
     5161 cccttctggc tgacctcctg cacggagtac tccagctggg ccggcagcgg gagcaggaac
     5221 ttgtggaacg tcgtctggag agaattgagg atgaaccggc gagccctccc ggacggggcc
     5281 agcagatacc gctctccaac ggcgagcgag atctgccgca ccgcctcggt tatcaccatg
     5341 gcggtcacat gggaaccggt ggtgtggtcg aggaccatct cctgccggtc cgaaatacgc
     5401 agagttgcct tgaagaggtc gtcctcgact ttccgcaacc cggcgatgag gacgttctcc
     5461 ggtttacgct tgtgcacctc ggaatggtag agcagttccg gtaggggaat gtccttgagt
     5521 atcgactccg gtaagccatg ctggcgcagc ttgctgcgga cgatttccct ttcgtaatcc
     5581 tcgatgccct gtcccgggat gatgaccggg aaaggctcgg aaatcagttc ccccttgagg
     5641 gaggccgtaa actcggacaa ggaaaccagg tcgaccgagt tcgacctggt ggccaaattg
     5701 tcgccgacga cgaacacgac cttttcgggc atgactccac ttccttcatt gaacccagga
     5761 gacacttaac gtccgattct tcctgctcac cttgtcgtcc gctggacgtt agggttgcga
     5821 tttacatgag gcgtggcgag ctgggcgtcg atcgcagaga acgcgtcgag gctggtgacg
     5881 tcacaacgtg ctcgatctgg atttcccgct gtccaggtca cgtcggcggc gggtgacttt
     5941 gatcgcccga tccttgctgt agggcggggc agggactgcg caccctccga ctcgaatcac
     6001 ctgatcgtgt acacgatccg tgacgtggac ttctcgatga cttctcccgc tgatgatcga
     6061 agcgcgactg agccgtccga agacgtcttc tgtgtctgtg tccttcggca tgcccggccg
     6121 catgcactgt ccgctcaaga gaaacggcac atcgtgcatt tgactccgca cgagcaggag
     6181 cgcctgctga tccatgtggc ggctgacgtg gcgcagcggc gccgtggcag tggcctgctc
     6241 ctcaattacc ccgaggtgat ggccctgttg accgtgcacg tctttgagca ggcgcgtgcc
     6301 ggcaagacgg tcagcgacat catggactcg ggccggcagt tgttgagccg gggcgaggtg
     6361 atggacggcg ttccagagat gatcaagaac gttcaggtgg aggccacgtt cccggacggc
     6421 accaagctgg tcaccatcca cgatcccttc ccggaagccg cggaggaacc cgaggtctac
     6481 ccgggcaagg tcgagcaccc ccggccgccc cgcaagccgg gctgcccggt cgactgcgac
     6541 ggcggcagcg gcaacgactc gtccccgtgc tgcgacaagt gccgagaggc ggaggcggtg
     6601 tcttggtacg aggcgattga gttcaacgca gacctacagg aagacgccac cacgctgccg
     6661 ggtgagggca ggacgaggat cagggtgaag aacatgtcgg accgacccat ccaggtcggt
     6721 tcccactatc acttcgccga ggtcaacccg gggctgaagg tcctcaaggt cgaggtccct
     6781 tccggtccgg agctggagcc gccccaggag ctcaaggact gcgaggcggc gcggatgcgg
     6841 cggctgaaca tcgccgcggg tacgtccgtg cgcttcgagc cgggtgacga gtgctgcgtc
     6901 gagttggtgg agatccaggg agaccgacag gtcaagggac tgcgcgagga gacgtaccag
     6961 tgagcgacaa ccagaccacc ccgtcgaaca gcaggtcggc gagctcaccg gggaacccgg
     7021 cgtcgagcaa catgctgaag cgggccgagt acgccactct gtacgggccc accaccaagg
     7081 accgcgtccg cctggccgac accgctctga ccgtcgaggt cgaggccgac tggagcggcg
     7141 gtccgaagca cagcggcaac gagatgatct tcggcggcgg aaaggtgatc cgcgagtcga
     7201 tgggaatgtc ccacatcccc agagacgggg atgccgaagg ccgcaagccc gtggacacgg
     7261 tcatcacagg tgcgctgatc ctcgactggt ggggggtcgt caaggcagac gtgggtatcc
     7321 gcggcggtga gatcgccgcc atcggcaagg cgtacaaccc cgagacgatg gacccgatcc
     7381 cggacgacga gttcgagacg cccgaccgta catccccgag cgacaagctg ccggtgcagc
     7441 agacgctgcc ggtgcagccg acgaacttcg tggtgggtcc gagcaccgag gtcatttccg
     7501 gcaacgggcg gatcctcacc gcgggcggcg tggacaccca cgtgcatttc atctgcccgg
     7561 agcagatcca cgaggccctg gcttcggggg tgaccacgct catcggcggc ggcaccggcc
     7621 ccgccgaggg cagcacggcc accacggtga ccccgggcga gtggcacatc acccgggtct
     7681 tcgggagcct ggacgcgtac ccggtcaaca tcggtctgct cggcaagggc agcacagtga
     7741 acaccgacgc cctcaacgaa caggtcgatg ccggtgtcat cggcttcaag gtccacgagg
     7801 actggggagc cacgcccgcc gtgatcaacg cggcactgga cgtgtgcgag agccgtcagg
     7861 tccaacttgc cctgcatgcc gactcgctga acgaatccgg tttcctggac agcacccgca
     7921 acgccttcac aaaggacggt aagaagcgcc cggtccacat cttccacgtc gagggcgccg
     7981 gcggtggtca cgccccggac atgatcgagc tggtcaaaga ggagaacgtg ctgcccgctt
     8041 cgaccaaccc gacccgcccc ctgacggtga acaccgtcaa ggagcatgtc gacatgatgg
     8101 tggtctgcca ccacctcaac ccggatattc cagcggacat ggccttcgcc gactcccgca
     8161 tccggccgtc caccatggcc gctgaggacc tcctccacga catgggcgcc atttcgatga
     8221 tgtcctccga cgcccaggcg atgggacgca tcggcgagat gatcatgcgc acctggcaga
     8281 ccgcgcacgt catgaaaacc cgctacggcc cactgaagga ggacctagag aacgcgaagg
     8341 tccgcccgat cgctgacgac accgaccact cgcacaacgc ggacaggctg ctcccgaacg
     8401 acaactaccg ggcgcgccgc tacgtcgcca agtacacgat caacccggcc atcacgcacg
     8461 gcatcgacgg tcacgtcggt tccgtggaga ccgggaagct cgccgacctg gtgctctggg
     8521 agccgaagtt cttcggcgtc aagatgcaca tggtcctcaa gggcgggcag ctcgcgtatg
     8581 cgcaggtggg cgacgccaac gcgtcgatca cgacaccgca gcccttcctg ccgcgggcgg
     8641 tctggggctc caccggccga tcccccctga gcaactcgta caacttcgtg gcaccgggag
     8701 tggccgacac gttgaactcg cgagtcgtca ccgtccagga cagcagcggg aacgtcaccg
     8761 agatgaagat cggtgggctc ggcctcgcca agaagttcgt ggacatctcg acgagtatcc
     8821 gggacatcac caaggccgac atgaagctga acgacacggt gccggagagc ctcaaggtcg
     8881 accacaacag cttcgaggtc accatcggcg gtgcgaccac aggggacgcc cgcacggaac
     8941 tcaacggagt gaccgtaccg cgcacctacg tcagtgaagt tcccatggcc cagaggtact
     9001 tcctcttctg actggccctt gcccggccga cgacgccgtc accgcggccg tggtccaggc
     9061 aggcacctgt gcccgccagg accacggcac accttccgac cctgcgaaag gcagccgctc
     9121 gcccatgcgc cgtgccgcac tgctcctgct ggccgacggc cgcttccccg ccggcgggca
     9181 cgcccactcc ggcggcgtcg aagccgcggt cgcccacaaa gccgtacacg acgtcgacag
     9241 cctcgaagcc ttctgccgcg ggcgcctgca caccaccgga ctgaccacgg ccggcctggc
     9301 tgccgcggcc gccgtcggat gcgatccgct gctgctcgac gatgccgccg acgcacgcac
     9361 ccccgccccc gcactgcgcg ccgtggcccg caggctcggc cggcagatga tgcgcgcggc
     9421 acgcgccacc ttcccgtccg ccgaactcga ccgcctggcc gccgagcgac cccagggtgc
     9481 ccatcagccc atcgtccagg gtctcgccgc ccaggccgcc gggctcaccc cgcaggacgc
     9541 cgcctgtgcc gcagcctacg agagcaccgg cggcccggcc accgcggcgg tgcgcctgct
     9601 cagcctcgat ccactcgacg cctcgcggct gctggcacgc ctcagcgcgg agatcgacac
     9661 cgtcgccgcg tccgccgccg acgcggcggc ccgcgtggtg accgagggcc ttgacgccct
     9721 gccgtcggcg tcctccccgc tgctggacat caccggagag cagcacgccg cctggaccgt
     9781 gcggctcttc gcctcctgaa cccaccccct gcctcctgga gctccatgca cctcgatcac
     9841 cccgtgacca tgccccaccg ccacacctac agcgccgagc cgcagcgcac ggacggcagc
     9901 cgccgcgcct tccgcgtcgg cctgggcggc cccgtcggtt ccggcaagac cgccagtgtc
     9961 gccgcactct gccgcaccct gcgcgacgaa ctctccctcg ccgtcgtcac caacgacatc
    10021 tacacccgcg aggacgccga gttcctgctg cgcgaggccg tgctgccacc cgagcggatc
    10081 accgcggtgg agaccggagc ctgcccgcac accgcgatcc gggacgacat ctccgccaac
    10141 ctggaggccg tcgagcactt cgacgagacc cttgaccccc tcgacctcgt gctcatcgag
    10201 tccggtggcg acaacctcac cgccaccttc tccaagggcc tcgtcgatgc gcagatcttc
    10261 gtcatcgacg tctccagcgg cgacgacatc ccacgcaagg gaggccccgg catcaccacc
    10321 gccgacctcc tcgtcgtcaa caagaccgac cttgctccgc acgtcggtgc cgacctcgcg
    10381 accatggccg ccgacgccaa acggcaacgt ggcgaccgtc ccgtcgtctt caccagcctc
    10441 acctccgacg acggcatccg cgagatcgcc gactgggtca ccgggcacct cacccggtgg
    10501 cgcgcgaagg cgtcggcatg aactccggcg cccaactctc cgaactcagg tcccggttcg
    10561 atgccgccgg tcccgccgcc ggtaccgagg aaacctgcgg acacccggac ggcgtgcgtg
    10621 ccaccgcccg catccgcgcc acgtacaacg gccgcgtcac cacgcttccg caactgcgca
    10681 gcgacggccc gttccatcta cgacgcatgc gcaccagcgg gaaggccgcc ag
//
