LOCUS       NZ_CP029541            12941 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. NEAU-S7GS2 chromosome, complete genome.
ACCESSION   NZ_CP029541
VERSION     NZ_CP029541.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NEAU-S7GS2
  ORGANISM  Streptomyces sp. NEAU-S7GS2
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Streptomyces sp. NEAU-S7GS2 isolated from saline-alkaline soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) School of Life Science, Northeast
            Agricultural University, Mucai Street, Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: AMRT Link v. v5.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003173275.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 05:28:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,458
            CDSs (total)                      :: 8,365
            Genes (coding)                    :: 8,052
            CDSs (with protein)               :: 8,052
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 313
            CDSs (without protein)            :: 313
            Pseudo Genes (ambiguous residues) :: 0 of 313
            Pseudo Genes (frameshifted)       :: 144 of 313
            Pseudo Genes (incomplete)         :: 230 of 313
            Pseudo Genes (internal stop)      :: 21 of 313
            Pseudo Genes (multiple problems)  :: 74 of 313
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:52:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9463181
            Orig. end                         :: 9476122
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12941
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="36"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12941
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12941
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7941](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7941
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    complement(601..616)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.32"
                     /tool="antismash"
     misc_feature    complement(618..631)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.02"
                     /tool="antismash"
     misc_feature    complement(1879..1899)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.36"
                     /tool="antismash"
     gene            3922..4776
                     /locus_tag="DKG71_RS41345"
                     /old_locus_tag="DKG71_41345"
     CDS             3922..4776
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /locus_tag="DKG71_RS41345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41345"
                     /product="Ig-like domain-containing protein"
                     /protein_id="WP_162597034.1"
                     /transl_table=11
                     /translation="MVSSPDPSVAGQAVTLTATVSALAPGAGTPTGTVTFGFGDGTSAA
                     TATLSGGTATVTHTFATRNGSPFPVTATYNGDTNFSASTGNDTQTLTKSATTTTVTSTP
                     DPSASGGTVTVRATVSSPAGAPTGTVTFSFGDGTGAATATLSGGVATVTHTYTTTTGSP
                     FTLTATYGGDASFTGSTGSDTQTVNRAATSTTVVSTPNPSTTGDRVTVTATVVPVAPGT
                     GTPTGTVTLAITGRTPQAVPLVGGTASVSFNPLQKGTHLVTGNYNGDVGFAPSAGTTVQ
                     TVP"
     misc_feature    4972..4989
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.27"
                     /tool="antismash"
     misc_feature    4979..4989
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     misc_feature    4982..4997
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.3"
                     /tool="antismash"
     gene            5001..6155
                     /locus_tag="DKG71_RS41350"
                     /old_locus_tag="DKG71_41350"
     CDS             5001..6155
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608091.1"
                     /locus_tag="DKG71_RS41350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41350"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_093635743.1"
                     /sec_met_domain="DAO (E-value: 1e-66, bitscore: 224.8,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTRRTSDVIVVGAGVVGAACAYYAARAGLSVTVLDRGPVAGGTT
                     GAGEGNLLVSDKEPGPELDLALLSTGLWRELAELLPPQIEYEAKGGLVVAAGEAGMRAL
                     RDFAAGQGKAGVRAEEVPGDRLQELEPHLTPGLAGGFHYPQDAQVMPALAAAHLLRAAG
                     DRVCLRLGEEVTGLLTAADGSVRGVRTVAGEVHAPHVVNAAGTWGGEFARLAGVGLPVL
                     PRRGFVLVTEPLPRVVRRKVYAADYVADVASGSAALQTSAVVEGTAAGPVLIGASRERV
                     GFDRTLSTEVLRRLAAQATALFPVLAGVRAMRTYVGFRPYLPDHLPAIGPDPRVPGLLH
                     ACGHEGAGIGLAPATGLLIARSLGGGELPLDPGPFDPARFEATV"
     gene            6183..6497
                     /locus_tag="DKG71_RS41355"
                     /old_locus_tag="DKG71_41355"
     CDS             6183..6497
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091458.1"
                     /locus_tag="DKG71_RS41355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41355"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_093635745.1"
                     /sec_met_domain="Fer2_4 (E-value: 5.7e-24, bitscore: 82.9,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARTPAELAGAHPGASFEITMDGRPVPALPGQSVAAALWSAGILA
                     WRTARTTGRPRGAFCGIGQCYDCLATINGEPNRRACLVPARPGDVITTQEGHGHARIGV
                     "
     misc_feature    6228..6241
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
     gene            6478..7941
                     /locus_tag="DKG71_RS41360"
                     /old_locus_tag="DKG71_41360"
     CDS             6478..7941
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091457.1"
                     /locus_tag="DKG71_RS41360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41360"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_109895527.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2.9e-22, bitscore:
                     78.2, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 3.1e-10, bitscore:
                     39.2, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPASASDPYDLAVIGAGCAGLAGAVAAAGHGLSVALLDASSQTGG
                     QFYRHPAPALGAARPEALHHDWPAFAALRRRLERSDVRQLSGHHVWAVTKEDGDLWAVH
                     AATGADGADERSVRVRARAVLLATGAYERQLPFSGWTLPGVVGAGGAQAMLKSGLVLPG
                     RRIVVAGSGPLLLAVASSLAAAGAQVPAVIEASGYQGYARHPRALAANPRKLAEAAVHG
                     AALLRHRVRMRTRSAVTEVHGTDRVSAVTVTQLDRDWRPVRGTGRRIACDALAVGHGLV
                     PQIELATALGCATRTTADGTLALALDGLQETSVRGLWAAGETGGVGGAELARAEGELAG
                     LAAAVRLHGHPVHEGRVRALRRRRDRMRAFAEAMAVSHAPAAGWTGWLTDDTDVCRCEE
                     VTAGRIREAVTDLGARDAHTVKLLTRAGMGWCQGRMCGTAAACLAAGGGAAEPPAERRP
                     FAVPVPLSALASPDEPTGSRPDAAPHVDD"
     misc_feature    7980..7990
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     gene            8015..8923
                     /locus_tag="DKG71_RS41365"
                     /old_locus_tag="DKG71_41365"
     CDS             8015..8923
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091456.1"
                     /locus_tag="DKG71_RS41365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41365"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_109895529.1"
                     /transl_table=11
                     /translation="MTTLTWNSIRPWRGIMVATALPLRDDLSVDHDAYARHVRWLIEQG
                     CDGVVPNGSLGEYQTLTDEERARVVRTAVEAAGDGARVMPGVAAYGSAEARRWAEQAAE
                     AGCGSVLLLPPNAYRADERAVRAHYTEVAKAGVPVVAYNNPLDTKVDLTPGLLARLHQD
                     GSIVAVKEFSGDVRRAYEIAELAPELDLLIGADDVLLELAVAGAVGWIAGYPNAFPATC
                     AELYHAAVAGDLETALPLYKSLHALLRWDSKTEFVQCIKLSMDLVGRRGGPARPPRSPL
                     TGEIEAEVRAATEKARAAGHH"
     gene            8955..9959
                     /locus_tag="DKG71_RS41370"
                     /old_locus_tag="DKG71_41370"
     CDS             8955..9959
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="DKG71_RS41370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41370"
                     /product="proline racemase family protein"
                     /protein_id="WP_093636444.1"
                     /transl_table=11
                     /translation="MRTRHVFHAVDSHTEGMPTRVITGGVGVIPGATMAERRLHFIEHL
                     DHLRTLLMYEPRGHSAMSGAILQPPTRADADFGVLYIEVSGLLPMCGHGTIGVATVLVE
                     SGMVPVTEPVTPVRLDTPAGLVSVDVHVADGVAKSVTLTNVPAFCAALDRTVEVPGYGR
                     VTYDLAFGGNFYAFVELDALGLPFDRARKDDLLAAGLAVMAAINASPDRPVHPEQPEIA
                     GVKHVYLAAPGSDARHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLDRDFVNE
                     SFIGTRFTGRLVAETEVGGLPAVVPTVTGRAWITGTAQYFLDPDDPFPGGFLL"
     gene            9956..11338
                     /locus_tag="DKG71_RS41375"
                     /old_locus_tag="DKG71_41375"
     CDS             9956..11338
                     /EC_number="1.2.1.-"
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091454.1"
                     /locus_tag="DKG71_RS41375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41375"
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_109895531.1"
                     /sec_met_domain="Aldedh (E-value: 5.9e-124, bitscore:
                     413.0, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAVSSVTSRNPADPSDVLVSFPAPGAFAAVAAVERARAAQPNW
                     STGGAAARSAALGAVAAALEAAADELTALAVREVGKPLTEARAEVARTVAIWRYYAQAP
                     YEPLGAVHETAAGPGLLLTRRRPYGVAGLLTPWNFPFAIPSWKAAPALAAGNTVVLKPA
                     PEATACARRLAALVEQALPAGVFTVLPGGATEGNALVSAADVVSFTGSTAVGRAVVHTA
                     TARGIPVQAEMGGLNAALVLPDADIERAALHIAAAIAGYAGQKCTATSRVIAVGAALDT
                     LREALPEALRAVAVGDPADASTVCGPLINEPARDRVSRAWDGLSVLAGGTVPGLPGWYA
                     APTLVEKVPPDHPLLREEVFGPVAALLPADDLAHAVRITNSVPYGLVTSVHSAGLDPVL
                     HGLDRLDTGMIRVNAPSTGVDFHLPFGGAKESGHGPREQGRAALEFYTSSRTYTLSPAE
                     TM"
     gene            11386..12072
                     /locus_tag="DKG71_RS41380"
                     /old_locus_tag="DKG71_41380"
     CDS             11386..12072
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091453.1"
                     /locus_tag="DKG71_RS41380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_41380"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_093635753.1"
                     /transl_table=11
                     /translation="MGHLKHRDLNATRERLRDQVAHALRAALISGELRPGEVYSAPVLA
                     EDFGISATPVREAMLDLAREGLVEPVRNKGFRVTEVNEHDLDQYTEIRALIEIPMIGRI
                     TRSAAREELEALRPVAEEIVRAARDHDLIGYLEADRQFHLELLGLSGNDRLVETVGELR
                     KRSRLYGLTGLDERGELIPSAEEHLELLDLMLAGNAKGAEKCMAKHLGHVRSLWARSGT
                     AAKSGR"
     misc_feature    12194..12210
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 18.86"
                     /tool="antismash"
     misc_feature    12198..12208
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.4"
                     /tool="antismash"
     misc_feature    complement(12204..12214)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     misc_feature    12648..12662
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
     misc_feature    12724..12734
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 17.67"
                     /tool="antismash"
ORIGIN
        1 agtccgccgg caccgcaacg cacagtgtgg agcaggccgc gaccaccacg acggtcaccg
       61 ccgctcccga cccgtccgtg acgggggagt cggtgaccgt caccgtgccc cccaccgcgc
      121 ccgcctcggg catcccgacc gggacggtca ccttcgactt cggtgacggc accccggtcc
      181 tcaccgccga gctcgtgggc ggcacggtga cggtcaccca cccctacacc agcgtctccg
      241 gcagccccta cacgattacc gcggtctacc tcggtgacgc caactacgcc gcgtccgagg
      301 acaccctcgt ccacgccgtc gccccggccg cgaccaccac cacggtgacc tcctcgcggg
      361 ttccctcggc ggtgggcgag agcgtgatgc tgatcgcgcg ggtaactccg ttgccgccag
      421 gggccggtgc accgacaggg acggtcacct tcgacttcgg tgacggcacc actcccgcca
      481 cggcagcggt ctcccacggc gtggcctcgg tgacgcacac ctacgtcagc accgcgggca
      541 gtccctaccc cgtcaccgcg acctacagcg gtgacaccga cttcgccgcc tccaccggga
      601 cgttcgccca gggcgtggag accagcgttt cggtcacctc caccacggtg agctccgctc
      661 ccgacccgtc cgtcgtggga caggcaatca ccgtcaccgc gaccgtcgtg gccgttccgc
      721 ccgcctcggg caccccggcc gggacggtga ccttcgaatt cggcgacggc accccaaccg
      781 tgacggcccc gctcacgggc ggcacggcga cggccaccca cacctacacc gcggccgccg
      841 atcccgccac gatcaccgcg gtctacaacg gtgacgggaa cttcgcgggt tcggccggcc
      901 tcgacaccca gacggtctcg caggcggcca cgtcgacgac ggtggcctcc gcgccggacc
      961 cgtccgtgac cggccagcac gtcaccttca ccgcgaccgt cacctcggcg gactcgggtg
     1021 ccggtacacc gaccggcacg gtcaccttcg acttcggcga cggcaccccg ggcctcacgg
     1081 cgcccctcac cgacggggtg gcgaccgccg tgcacgccta cccccacacc tccggcagcc
     1141 cgtacccggt caccgccacc tacaacggcg gggcgagctt cacgccttcc acggacaccg
     1201 acacccagac cgtgaacccg gcgaccgcgt ccctcaccgt cttctcgtcg gcggcccctt
     1261 ccacggtcgg ccagccggtg accctcaccg cgaacctcac ggcggatgcc ccggcggcgg
     1321 gcaccccgac cgggacgatc accttcgact tcggcgacgg caccccgacc gtcaccgcca
     1381 ccgccgtggc cggcacggtg acggtcactc atgcgtacac caccacggcc ggcagcccgt
     1441 tcaccctctc ggccacctac agcggggacg aagacttcag ccccgccaca gccaccgcct
     1501 cccagaccct gtacccggcc tcgaccacca ccgaggtgac ctccggtccg aacccctcgg
     1561 tcgtggggga gccggtgacc gtcaccgcga cggtcgcatc ggtcccgccg ggtgccggca
     1621 caccgaccgg tacggtcacc gtcgacttcg gcgatgccac cactccggcc acggcccccc
     1681 tcgccggtgg agccgtcacg ctcacccaca cctacaccgg cagcgtcagc agcccgtaca
     1741 cgatcaccgc caccgccacc tacagcggcg acaccgattt caccgcttcc agcgacacca
     1801 ccacgcagtc cgtcggccag gccgtgtcca cgacggtggt caccacgtcg cccgaaccgg
     1861 cggtgaccgg ccagccggtg accgtcaccg cgaccctctc ggccgccgcg ccgggggacg
     1921 ggacgccgac cggcacggtc accttcgact tcggtgacgg tacgtcaccc ctcacggtgc
     1981 cggccacggc cggggtggcc acgacgacgc acacctgggc cggtacgtcc ggcagcccgt
     2041 acaccgtcac ggccgactac agcggggacg ccggcttcac cccgtccacc ggcaccggca
     2101 tccagaccgt ggccccggcc gcgaccttca cctccgtcag cggagtgccc gagccttcgg
     2161 tgacgggcca gccggtggac atcaccgcga cggtctccgt ggtcgccccg ggggccggta
     2221 cccccaccgg ttcggtcacc ttcgacttcg gtgacggcac cccggccgtc accgcacccg
     2281 tgacggacgg caccgccacc ctcacccaca cctgggcggg cacctccgcc ggcccgtaca
     2341 ccgtcacggc cacctacagc ggggacaccg acttcagccc ctccaccggt accgacaccc
     2401 agaccgtggc cccggccgcg accaccacca ccgtcagcgg actgccggag ccctcggtga
     2461 ccggccagcc ggtgacgttc ctcgccaggg tgacgtccga agccccgggc gatggcgcgc
     2521 cgacggggac ggtgaccttc gacttcgggg acgggaccgc gccggtcacc gtcaccgcag
     2581 cgggtggggt ggcgaccgcg gtgcacgcgt acacgagcgc cacgggcggc ccttacacgg
     2641 tccacgccgc ctacagtggc gacgaccagt tcaccgcctc cgtggggacc aaaacccaca
     2701 tcgtgcagcc ggcgatgtcg atgacggtgc tcagctcgtt gccggatccc gcggtgacgg
     2761 gccagccggt ggagatcacc gcgacggtct ccgcggccgc cccgggggcg ggcgccccca
     2821 ccggtacggt caccttcgac ttcggcgacg gcaccccctc cgtcaccgcc acggtcaccg
     2881 cgggcgtggc aaccgtgacg cacgcctggg ccggtacgtc cggcagcccg tacaccgtca
     2941 cggccgacta cagcggggac accgacttca actcctccac cggcaccgac agtcagaccg
     3001 tcggtcagac ctcttcctcc atgaccctgt tcacctcccc ggatccgtcc gtggccgggc
     3061 agcaggtcac cttcaccgcc cgggtcaccg ccgtccctcc gggcgcgggc acccccaccg
     3121 ggaccgtcac cttcgatttc ggcgacggca ccgcacccgt caccgcaccg gtctcctccg
     3181 acgaggcgac cgtgacgcac gcctacgcga gcacggcggg cagcccctat acggtcaccg
     3241 ccacctacag cggtgacgcc gacttcgccc ccgccaccac caccggagac cacacggtgg
     3301 cggtcagcgc cgccaccacc tcgacgaccg tgagctcctc cccggacccc tcggtaacgg
     3361 gccagccggt gaccttcacc gcgaccgtga caccgacccc gcccggcgcc ggggtgccca
     3421 ccggaacggt ttccttcacc ttcggcgacg gaacggcggc cctcacggca ccggtcaccg
     3481 gcggggtggc gaccgtcacg cacgcctaca ccaccgcgac gggcagcccc cacaccgtga
     3541 ccgcgaacta cagcggggac gccaacttca gctcctcctc gggcacggac agccagacgg
     3601 tcgatccggc gtcgacgctg acgacggtga ccgccacgcc gggcccgtcc gtggtcggcc
     3661 agccggtgac cttcaccgcg accgtcacac cggtcgagcc gggagccggg gtgccggccg
     3721 gcgcggtcac cttcgacttc ggggacggca caccgcccac accgacattc ggtttccgac
     3781 ggcgtcgcca ggatcacgca cacgtacacg agcgcgggcg gcccctacac ggtgaccgcg
     3841 cagtatgagg gggacgacag cttcacgtcg tcgagtggga gtgacaccca gacggtcggc
     3901 agggcggcta cgacgacggc ggtggtgtcg tcgccggatc cgtcggtggc ggggcaggcg
     3961 gtgacgctga cagcgacggt ctctgcgctg gcgccgggtg cggggactcc cacggggacg
     4021 gtcaccttcg gtttcgggga cggcaccagc gccgccaccg cgacgctctc cggcggcacc
     4081 gcgaccgtca cccacacctt tgccaccagg aacggaagtc ccttccccgt cacggccact
     4141 tacaacgggg acaccaactt ctcggcctcc accggcaacg acacccagac gctcaccaag
     4201 agcgcgacca ccaccacggt gacctccact cccgacccct cggcgtccgg cgggacggtg
     4261 accgtccggg cgaccgtctc gtccccggcc ggtgcgccga ccgggacggt caccttcagt
     4321 ttcggtgacg gcaccggcgc cgccaccgcg acgctctccg gcggtgtcgc gaccgtcacc
     4381 cacacctaca cgaccaccac cggaagcccg ttcaccctca cggccaccta cggcggggac
     4441 gcgagcttca cgggctccac cggcagtgat acccagaccg tcaaccgggc ggcgacctcc
     4501 accaccgtgg tctccacccc caacccctcg acgacgggtg accgggtcac ggtcaccgcg
     4561 accgtggttc cggtcgcccc cggaacgggt acgcccaccg gaaccgtcac cctcgccatc
     4621 acgggacgca ccccccaggc cgtgcctctc gtcggcggca cggccagcgt gtccttcaac
     4681 ccgctgcaga aggggaccca cctcgtcacc ggcaactaca acggcgacgt cggtttcgcg
     4741 ccctccgccg gaaccacggt ccagacggtg ccatgacgga gcggtgcccc ggatcccgtg
     4801 ccggatccgg ggcatcaccg ggttcagacc gcctcgggga agtggcagac gccggagagg
     4861 tgttggcgcg tccgttactt acccgggtcc gaggggtcct ggagaatcgg gacgcctcga
     4921 cggccgtccg tcccggggga gttgcggccg tatgacgcgc cacccttggc atcgtcagtg
     4981 caatgtgaaa tattactgac gtgcccacga gaagaacctc ggatgtcatc gtcgtcggcg
     5041 cgggcgtggt cggcgcggcc tgcgcctact acgcggcccg cgccggcctc tccgtcaccg
     5101 tgctcgaccg cggccccgtc gcgggcggca ccacgggtgc cggcgagggc aacctcctgg
     5161 tctccgacaa ggaacccggg cccgaactcg atctcgccct gctgtccacc gggttgtggc
     5221 gggaactggc ggaactgctc ccgccgcaga tcgagtacga ggccaagggc ggcctcgtgg
     5281 tcgccgccgg tgaggccggg atgcgggctc tgcgcgactt cgcggccgga cagggcaagg
     5341 ccggtgtgcg ggcggaggag gtcccggggg accggttgca ggagctggag ccgcatctga
     5401 ctcccggtct ggcagggggc tttcactacc cgcaggacgc gcaggtgatg ccggccctgg
     5461 ccgccgcgca tctgctgcgc gcggcgggtg accgggtgtg tctgcggctc ggtgaggagg
     5521 tcaccggcct gctgaccgcg gcggacggtt cggtgcgggg ggtgcgtacg gtggcgggtg
     5581 aggtgcatgc tccgcacgtg gtcaatgcgg ccggtacctg gggcggcgag ttcgcccggc
     5641 tcgcgggcgt cgggctgccg gtgctgccgc gccgcggctt cgtgctcgtc acggaaccgc
     5701 tgccgcgggt ggtgcggcgc aaggtctacg cggccgacta cgtggccgat gtcgccagcg
     5761 gttcggcggc gctgcagacc tcggcggtgg tcgagggcac cgcggcgggc cccgtcctga
     5821 tcggtgccag ccgggaacgg gtcggtttcg accgtacgtt gtcgaccgag gtgctgcgcc
     5881 gcctggcggc ccaggccacc gccctgttcc cggtcctggc cggggtccgc gcgatgcgga
     5941 cgtacgtggg gttccgcccc tacctccccg accatctgcc ggccatcggc cccgatccgc
     6001 gggtgcccgg cctgctgcat gcctgcggcc acgaaggcgc ggggatcggt ctcgccccgg
     6061 ccaccgggct gctcatcgcg cggtccctgg gcggcggtga actgcccctg gacccgggcc
     6121 cgttcgaccc cgcccgcttc gaagccaccg tctgacccgt ccactaggag aggaggcccg
     6181 cagtggcccg tacccccgcc gaactggcag gggcgcaccc cggcgcgtcg ttcgagatca
     6241 cgatggacgg ccgtccggtc cccgcgctgc ccggccagtc cgtcgccgcc gccctgtggt
     6301 ccgccggcat cctcgcctgg cgcaccgccc gcaccaccgg ccgcccgagg ggagccttct
     6361 gcggcatcgg acagtgctac gactgcctcg ccaccatcaa cggggagccc aaccgcaggg
     6421 cctgtctggt gcccgcccgc cccggcgacg tgatcaccac ccaggaggga cacggccatg
     6481 cccgcatcgg cgtctgaccc gtacgacctc gcggtgatcg gagcgggctg cgccggtctc
     6541 gccggcgccg tcgcggccgc cggacacggc ctgtccgtag ccctgctgga cgcctcgtcg
     6601 cagaccggcg ggcagttcta ccggcatccg gcgccggccc tcggcgcggc ccggcccgag
     6661 gcgctgcacc atgactggcc ggccttcgcc gcactgcgcc gccggctgga gcggagcgac
     6721 gtccgccagc tgtccgggca ccacgtctgg gccgtcacga aggaggacgg tgacctgtgg
     6781 gcggtgcacg ccgccaccgg ggccgacggc gccgacgagc ggtcggtgcg ggtgcgggcc
     6841 cgtgcggtcc tgctggcgac cggcgcgtat gaacgtcaac tccccttctc cggatggact
     6901 ttgcccgggg tcgtgggtgc cgggggagcg caggccatgc tcaagtccgg cctggtgctg
     6961 cccggccggc gcatcgtcgt ggcgggcagc ggccccctgc tcctcgcggt cgcctcctcg
     7021 ctggccgccg ccggtgcgca ggtccccgcc gtgatcgagg catccggcta tcagggatac
     7081 gcgcgccatc cccgtgcgct cgcggccaac ccgcggaaac tcgccgaggc ggcggtgcac
     7141 ggtgccgctc tgctgcggca ccgggtacgg atgcgcaccc gcagcgcggt caccgaggtg
     7201 cacggcaccg accgggtctc ggccgtcacc gtcacccaac tcgaccgcga ctggcggccc
     7261 gtacggggga cgggccggcg gatcgcatgc gacgcgctcg cggtgggaca cggcctcgtc
     7321 ccgcagatcg aactggccac cgcactcggc tgcgccaccc gcaccaccgc ggacgggacc
     7381 ttggccctgg ccctggacgg ccttcaggag acctcggtac gcggtctgtg ggcggcgggg
     7441 gagaccgggg gagtgggcgg cgccgaactc gcccgcgcgg aaggggagct ggccgggctg
     7501 gcggcagccg tcagactcca cgggcacccc gtccacgaag ggcgtgtgcg ggcgctgcgg
     7561 cgccggcggg accggatgcg tgccttcgcc gaggcgatgg ccgtcagtca cgcaccggcg
     7621 gcgggctgga cgggctggct gaccgacgac acggatgtct gccgctgcga ggaggtgacc
     7681 gccggaagga tccgcgaggc cgtcaccgac ctcggcgccc gtgacgcaca caccgtcaaa
     7741 ctcctcacca gagccggtat gggctggtgt caggggcgga tgtgcgggac ggccgcagcg
     7801 tgcctggcgg caggcggagg agctgcggaa ccacccgccg aacgccgccc gttcgcggtg
     7861 cccgtgccgc tctcggcgct cgcctcgccc gacgaaccca ccgggagccg gccggacgcc
     7921 gccccgcacg tcgacgactg acccgcgggc cgcccttgtg ggtgccgcgg acggcccaat
     7981 aaaatgtcac acactataga agggtttcac tggtatgacc accctcacct ggaacagcat
     8041 ccgcccctgg cgcgggatca tggtcgccac ggcgctgccc ctgcgcgacg atctctccgt
     8101 cgaccacgac gcctacgccc ggcacgtccg gtggctgatc gagcagggct gcgacggtgt
     8161 cgtaccgaac ggctcgctcg gcgagtacca gaccctcacc gacgaggagc gggcccgtgt
     8221 ggtgcggacc gcggtcgaag cggcgggcga cggtgcgcgg gtcatgcccg gcgtcgccgc
     8281 ctacggcagc gccgaggccc ggcgctgggc ggagcaggcg gccgaagcgg gctgcggctc
     8341 ggtcctcctg ctgcccccga acgcctatcg cgccgacgaa cgggccgtcc gcgcccacta
     8401 caccgaggtc gcaaaggcgg gggtgccggt cgtcgcgtac aacaaccccc tcgacaccaa
     8461 ggtggatctg acacccggac tgctggcccg gctgcaccag gacggcagca tcgtcgcggt
     8521 caaggaattc tccggcgatg tacggcgcgc ctacgagatc gccgaactcg ccccggaact
     8581 cgacctgttg atcggcgcgg acgatgtcct gctcgaactc gccgtggccg gtgcggtcgg
     8641 ctggatcgcc ggatacccca acgccttccc cgccacctgc gccgagctgt accacgccgc
     8701 cgtcgccggt gacctggaga ccgccctgcc gctctacaag tccctgcacg cgctgctgcg
     8761 ctgggattcg aagaccgagt tcgtccagtg catcaagctg tccatggacc tcgtcggccg
     8821 ccgcggtggc cccgcccgcc cgccgcgctc cccgctcacg ggcgagatcg aggccgaggt
     8881 ccgggccgcc accgagaagg cccgggccgc cggacaccac tgaccgcatc cgtgccgctt
     8941 cgaaaggact catggtgcgt acgcgtcatg tcttccacgc cgtcgactcg cacaccgagg
     9001 gcatgcccac gcgcgtgatc acggggggcg tcggtgtgat ccccggtgcc accatggccg
     9061 agcgcaggct ccacttcatc gagcacctcg accatctcag gaccctcctg atgtacgagc
     9121 cgcgcgggca ctccgccatg agcggcgcca tcctccagcc gcccacccgc gccgacgccg
     9181 acttcggcgt cctctacatc gaggtctccg gcctgctgcc gatgtgcggg cacggcacca
     9241 tcggggtcgc caccgtcctc gtcgagtccg gcatggtgcc ggtgaccgag ccggtcaccc
     9301 cggtgcggct cgacaccccg gccggcctgg tctccgtcga cgtacatgtg gcggacgggg
     9361 tggcgaaatc ggtcaccctc accaatgtgc ccgccttctg cgccgccctc gaccgcacgg
     9421 tggaggtccc ggggtacggg agggtcacct acgacctcgc cttcggcggc aatttctacg
     9481 ccttcgtcga actcgacgcg ctggggctgc cgttcgaccg cgcccgcaag gacgacctgc
     9541 tggctgccgg actggccgtc atggcggcga tcaacgcgtc gccggaccgc cccgtccacc
     9601 ccgagcaacc ggagatcgcc ggggtcaagc atgtctatct ggccgctccc ggctcggacg
     9661 cccgccactc gcggcatgcg atggcgatcc acccgggctg gttcgaccgc tcgccctgcg
     9721 gcaccggcac ctcggcacgg atggcacagc tgcacgcccg cggtgaactg cccctggacc
     9781 gcgacttcgt caacgagtcc ttcatcggca cccgcttcac cggccggctc gtcgccgaga
     9841 cggaggtcgg cgggctgccc gccgtggtac cgaccgtcac cggccgcgcc tggatcaccg
     9901 gcaccgccca gtacttcctc gacccggacg accccttccc cggaggcttc ctcctgtgac
     9961 caccgccgtc tcctccgtca cctcccgcaa cccggccgac ccttccgatg tgctggtgtc
    10021 gttcccggcg cccggagcct tcgccgcggt ggctgccgtc gagcgggccc gtgcggccca
    10081 gcccaactgg tcgaccggcg gtgccgccgc ccgttcggcc gccctgggcg cggtcgccgc
    10141 cgcactcgaa gccgccgcgg acgaactgac cgcgctcgcc gtacgcgagg tgggcaagcc
    10201 gctcaccgag gcgcgcgccg aggtcgccag aaccgtcgcg atctggcgct actacgccca
    10261 ggccccctac gaacccctcg gcgccgtgca cgaaaccgcc gccggcccgg gcctgctgct
    10321 gacccgacgc cgtccctacg gagtcgccgg cctcctcacc ccgtggaact tcccgttcgc
    10381 cattccgagc tggaaggccg ccccggcgct cgccgcgggc aacacggtgg tcctcaaacc
    10441 cgcccccgag gcgacggcct gcgcccggcg cctcgccgcc ctcgtcgagc aggccctgcc
    10501 ggccggggtg ttcaccgtgc tgcccggcgg agcgaccgag ggcaacgcac tcgtctccgc
    10561 cgccgatgtc gtctccttca ccgggtcgac ggccgtcggc cgggcggtcg tgcatacagc
    10621 cacggcccgg ggcatccccg tccaggccga gatggggggc ctgaacgcgg ccctggtcct
    10681 cccggacgcg gatatcgaac gggccgcgct ccacatcgcg gccgcgatcg ccggatacgc
    10741 gggccagaag tgcaccgcca ccagtcgcgt gatcgcggtc ggcgcggccc tcgacaccct
    10801 gcgggaggcg ctcccggaag ccctgcgcgc agtggccgtc ggtgacccgg ccgacgcctc
    10861 caccgtgtgc gggccgctca tcaacgagcc cgcacgcgac cgggtcagca gagcctggga
    10921 cgggctctcg gtcctcgcgg gcggcaccgt gcccggcctt cccggctggt atgcggcccc
    10981 gaccctggtc gagaaggtgc caccggacca cccactgctg cgcgaggagg tcttcgggcc
    11041 ggtcgcggcg ctgctgcccg ccgacgacct ggcccacgcg gtccggatca ccaactcggt
    11101 cccgtacggc ctggtcacct cggtccactc ggccggcctg gacccggtcc tgcacgggct
    11161 cgaccggctc gacaccggaa tgatcagggt caacgccccc tccaccgggg tcgacttcca
    11221 cctgccgttc ggcggcgcca aggaatccgg ccacggaccg cgggaacagg gccgcgccgc
    11281 cctggagttc tacacctcca gccgtacata caccctgtca cctgcggaaa caatgtgaaa
    11341 ttgtatgctg gtgatccgct ccggcatgaa gggatcacca ccgttatggg gcacctgaag
    11401 caccgcgatc tcaacgccac ccgggagcgg ctgcgcgacc aggtcgccca cgccctgcga
    11461 gccgccctga tctccgggga actgcggccg ggggaggtgt actcggcgcc ggttctggcc
    11521 gaggacttcg ggatctcggc cacacccgtg cgcgaggcga tgctcgacct ggcgcgcgag
    11581 ggcctggtcg agcccgtccg caacaagggc ttccgggtca ccgaagtcaa cgagcacgac
    11641 ctcgaccagt acaccgagat ccgcgcgctc atcgagatcc ccatgatcgg caggatcacc
    11701 cgctccgccg cccgtgagga actggaggcg ctgcggccgg tcgccgagga gatcgtgcgc
    11761 gccgcgcgcg accacgacct catcggctac ctggaggccg accggcagtt ccacctcgaa
    11821 ctgctcggcc tcagcggcaa cgaccgcctc gtcgagaccg tcggtgagct gcgcaagcgc
    11881 tcgcgcctgt acgggctcac cggcctggac gagcggggcg agctgatccc gtcggccgag
    11941 gaacacctcg aactcctcga cctcatgctg gcgggcaacg cgaagggcgc cgagaagtgc
    12001 atggccaagc acctgggtca tgtccgctcg ctctgggcca ggagcggaac cgcggcgaaa
    12061 tccggtaggt agcggcccgg ccggagcgcg gcccgctgag gcgagccccc ctcgctgacg
    12121 tgacgtgggg agggggctcc ccgacgtgac gcagaggggg ctccccgccg acgacgggcc
    12181 gcaccctgcc tgtttcatgc aatgtcacat ttcatcctgc cctcgggctg tgaaagggac
    12241 cgacccatga accccgccta caccaccgtc gatcacgtct tcaccgttcc cctggaccac
    12301 tccgcccccg acggggagga catacaggtc ttcgcccgcg aggtcgtcga cccggcccgc
    12361 acgggcgagc ggctgccctg gctgctgtat ctgcaaggcg gtccgggcgg caaatccccc
    12421 cgcccgtcag ccggttcacc cggctggctg aaccaggcgc tcacgaccca ccgggtgctc
    12481 ctgctcgatc agcgcggcac cggccgttcc accccggtca ccgcccgagc cgccgcccgc
    12541 ttcgcttccc ccgcccaact cgccgcgtac ctgggccact tccgcgccga ctcgatagtg
    12601 gccgacgccg aactgatccg ccggcgactc tgccaggacg aaccctggga gacgctcggc
    12661 cagagctatg gcggcttcct caccctcacc tacctctccc gcgcccccga gggcctgcgg
    12721 gcctgctatg tgaccggtgg tctgcccggt ctcaccgcca ccgccgacga cgtctacgcc
    12781 cgtacctacc cgcgggtcca agaccgcgtc ctcgacttct acgcccgcta cccggacgac
    12841 gccgcccgcc tccgcaggat tgccgatctc ctcgcgacca ccgaagtccg actgcccgac
    12901 ggggaccggc tcaccgtccg ccggctgcgc accctcggcc t
//
