LOCUS       NZ_CP029541            10984 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. NEAU-S7GS2 chromosome, complete genome.
ACCESSION   NZ_CP029541
VERSION     NZ_CP029541.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NEAU-S7GS2
  ORGANISM  Streptomyces sp. NEAU-S7GS2
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Streptomyces sp. NEAU-S7GS2 isolated from saline-alkaline soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) School of Life Science, Northeast
            Agricultural University, Mucai Street, Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: AMRT Link v. v5.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003173275.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 05:28:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,458
            CDSs (total)                      :: 8,365
            Genes (coding)                    :: 8,052
            CDSs (with protein)               :: 8,052
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 313
            CDSs (without protein)            :: 313
            Pseudo Genes (ambiguous residues) :: 0 of 313
            Pseudo Genes (frameshifted)       :: 144 of 313
            Pseudo Genes (incomplete)         :: 230 of 313
            Pseudo Genes (internal stop)      :: 21 of 313
            Pseudo Genes (multiple problems)  :: 74 of 313
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:52:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9120998
            Orig. end                         :: 9131982
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10984
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="33"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10984
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10984
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5984](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5984)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            129..356
                     /locus_tag="DKG71_RS39785"
                     /old_locus_tag="DKG71_39785"
     CDS             129..356
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608014.1"
                     /locus_tag="DKG71_RS39785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_39785"
                     /product="hypothetical protein"
                     /protein_id="WP_018092057.1"
                     /transl_table=11
                     /translation="MTEHPPVIVHPPTPSGGRRVTVRGQIVGLAHGRGDVAEFLRRAGV
                     ASPAGDIELDDPRLVEWRGGSLDDWPMPPP"
     gene            522..728
                     /locus_tag="DKG71_RS39790"
                     /old_locus_tag="DKG71_39790"
     CDS             522..728
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092056.1"
                     /locus_tag="DKG71_RS39790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_39790"
                     /product="hypothetical protein"
                     /protein_id="WP_109895175.1"
                     /transl_table=11
                     /translation="MGRPSAGELTAGEREHDVSDDGTSDDGGYSPLRGGSGGSSDLNVL
                     RRAMRWETFALVAFLVILAIVAS"
     gene            1100..2620
                     /locus_tag="DKG71_RS39795"
                     /old_locus_tag="DKG71_39795"
     CDS             1100..2620
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092055.1"
                     /locus_tag="DKG71_RS39795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_39795"
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="WP_109895177.1"
                     /transl_table=11
                     /translation="MNEDSRYEAVRSRDARFDGAFFFAVSTTGIYCRPSCPATTPRRRN
                     VAFFPTSAAAQGSGFRACRRCRPDAVPGSPEWNARADLVGRAMRLISEGVVDREGVCGL
                     AARLGYSARQVQRQLNAELGAGPAALARAQRAHTARVLLQTTGLQAAEIAFAAGFASVR
                     QFNDTIKEIYALTPSGLRAARPGKGTRFGPVAPPTTPGVLPLRLAFRGPYAARQLFDHL
                     QRRAITGVEEVSGEPGARTYRRTLRLPHGTGIAEVDEAAGDGWLDCRLRLTELRDLTTA
                     SQRVRRLFDLDADPYAVAERLGADSVLARLVDGCPGLRSPGAPAPDELAVRAVLGQQVS
                     VAAGRTLGGALVAAHGEPLPEPAGALTHLFPRIEDLAQATLTELGMPESRRATLRTLSA
                     ALADGTVVLDAGADRDEAERALLGLRGIGPWTAGYIRMRALGDPDVLLTGDAAVLAGMR
                     RAGVPAAGLKERATSWRPWRSYAMHHFWNAPATEPANPRPTTTESTGP"
     gene            2617..3141
                     /locus_tag="DKG71_RS39800"
                     /old_locus_tag="DKG71_39800"
     CDS             2617..3141
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092054.1"
                     /locus_tag="DKG71_RS39800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_39800"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_093635350.1"
                     /transl_table=11
                     /translation="MTLYTTLDSPLGELLLVGEESATAPGGLALASLSVPGQKGGATVQ
                     DGWTYAPAAFADIAHQLRAYFDGKLTRFGIAYAPARGTDFQRRVWQALEAIPYGTTTTY
                     GKIAADIGAARGAVRAIGTAIGANPLLVVRPCHRVIAADGSLSGYAGGPVRKRQLLGIE
                     GALPDVMPDAL"
     gene            3198..3989
                     /locus_tag="DKG71_RS39805"
                     /old_locus_tag="DKG71_39805"
     CDS             3198..3989
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092053.1"
                     /locus_tag="DKG71_RS39805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_39805"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_109895179.1"
                     /transl_table=11
                     /translation="MTTTTTATTTTTKRHARPATLAQRVAAADWTALADELNTVGCAVT
                     PRLLSPADCRALSALYDAPAPDHGAESGSRARFRSTIDMARHRFGSGQYRYFADPLPET
                     VQALREAFYPHLLKTARDWADKLGRPAPWPDTLEEWLTLCHEAGQTKSAQILLRYGEGD
                     WNALHRDIFGEMVFPLQVVIGLDEQGTDYTGGEFLLVEQRPRAQSRGTTTVLPQGHGLI
                     FTTRDRPVRSARGWSAGPVRHGVSTVRSGRRHSLGLVFHDA"
     gene            complement(4033..4797)
                     /locus_tag="DKG71_RS39810"
                     /old_locus_tag="DKG71_39810"
     CDS             complement(4033..4797)
                     /NRPS_PKS="Domain: PKS_KR (7-175). E-value: 1.1e-09. Score:
                     30.4. Matches aSDomain:
                     nrpspksdomains_DKG71_RS39810_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="DKG71_RS39810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_39810"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_109895181.1"
                     /transl_table=11
                     /translation="MSDLKGKTALVTGSSRGIGRGIAQRLAADGALVAVHYGSNDTAAK
                     ETAESIIRAGGRAFIVGAELGVPGDAEALYAAFDDGLRAYGAEPGLDILVNNAAISIPG
                     HIDEVTPEDFDRTLAVNTKAPFFIAQHGLRRMRDGGRIINVSSAVTSTAFPSTIAYGVS
                     KGAVDTFTLTLAKDLGARGITVNTIAPGFIETDMNAAMRATPEAHAALASLSVFNRIGK
                     PQDIADVAAFLASDDSRWITGQRFDVTGGSML"
     aSDomain        complement(4273..4776)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DKG71_RS39810_PKS_KR.1"
                     /evalue="1.10E-09"
                     /label="DKG71_RS39810_PKS_KR.1"
                     /locus_tag="DKG71_RS39810"
                     /protein_end="175"
                     /protein_start="7"
                     /score="30.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGIGRGIAQRLAADGALVAVHYGSNDTAAKETAESII
                     RAGGRAFIVGAELGVPGDAEALYAAFDDGLRAYGAEPGLDILVNNAAISIPGHIDEVTP
                     EDFDRTLAVNTKAPFFIAQHGLRRMRDGGRIINVSSAVTSTAFPSTIAYGVSKGAVDTF
                     TLTLA"
     CDS_motif       complement(4705..4770)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DKG71_RS39810_0001"
                     /evalue="3.00E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="DKG71_RS39810"
                     /protein_end="31"
                     /protein_start="9"
                     /score="10.3"
                     /tool="antismash"
                     /translation="LVTGSSRGIGRGIAQRLAADGA"
     gene            complement(5001..5984)
                     /locus_tag="DKG71_RS39815"
                     /old_locus_tag="DKG71_39815"
     CDS             complement(5001..5984)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="DKG71_RS39815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_39815"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_109895183.1"
                     /sec_met_domain="AfsA (E-value: 3.6e-18, bitscore: 64.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTMQVSGTLQPRSPHQLHLNGDRAATPRSRRSVQVTREFVHRP
                     LADDILVTSWRRLDDAHFSLTAQWPHDHGYFTPNRGRHHLILTGETIRQAGLLLCHTEL
                     GVPLGHHFILGDLVYTTHPEHLAVGSGPTPLTIDVACSRMRRRSGMMTSGHFDMTIRKA
                     GRIVATGHSDVTVTSPAVYRRIRGKRLTARRTFDPLPAPVPPRLTGSAKDSDVLLSPTG
                     RTDRWLLRIAPGHPAVVNPANDHIPGMALLDAAQQAARVLTSPDAFVPYAFGTVFRRYA
                     EHGTPCVIEARRVPSALPCTTTVQVTGLQDGDPVFVSTLTALDPRD"
     misc_feature    complement(6046..6059)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.3"
                     /tool="antismash"
     gene            6159..6845
                     /locus_tag="DKG71_RS39820"
                     /old_locus_tag="DKG71_39820"
     CDS             6159..6845
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092050.1"
                     /locus_tag="DKG71_RS39820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_39820"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_093635358.1"
                     /transl_table=11
                     /translation="MAQQDRAIRTRRVILEAAASVFDEQGYDRATIAEVLERAGVTKGA
                     LYFHFASKEQLALAVLEEQVVDIAVEPQKIKLQEFVDSGQVLAFRLRRDPIQRGAARLA
                     VEQGSNHLDRKQSMLSWTRFVEGLLNEARDRGEILESIVVRETAELFVGAFAGLQMMSQ
                     ALTNQADLSRRLTVFFEHTLPSIAVPAILAKLNLDSERGAQLDAALQGKALAKAVAGAD
                     AEVAAG"
     misc_feature    complement(6912..6921)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.85"
                     /tool="antismash"
     misc_feature    complement(6961..6976)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.64"
                     /tool="antismash"
     misc_feature    complement(7197..7212)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.38"
                     /tool="antismash"
     misc_feature    7198..7211
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.18"
                     /tool="antismash"
     gene            7522..10089
                     /locus_tag="DKG71_RS39825"
                     /old_locus_tag="DKG71_39825"
     CDS             7522..10089
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574653.1"
                     /locus_tag="DKG71_RS39825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_39825"
                     /product="MFS transporter"
                     /protein_id="WP_204166011.1"
                     /transl_table=11
                     /translation="MNRTTTDQLPERTVGTPAVPPVSDPAVGGKRRSSGGVVPVLAFSG
                     IVVAVMQTLLVPVIKDLPVLLNTTAGNATWVMTATLLAGAVSTPIMGRLGDLYGKRRML
                     LTSLAVMVVGSLICGFTSDLVTMIVGRALQGFAMGAIPLGIGIMRDELPRERLGSAMGL
                     MSSSIGVGGGLALPAAALVAQHADWHALFFGAAGLGVLSMLLTLLVVPETAVRAQGRFD
                     VTGAIGLSAGLVALLLPLTKGSDWGWGSPTTLVLFGVAVLILVLWGVMELRLADPLVDL
                     RTTARREVLLTNLASITVGVAFYAISLVLPQLLQLSTSTGYGLGQSMVVAGLCMAPLGL
                     TMMFVAPLYARIAARRGPKVSLLLGMLVIAIGYGAGIGLMNAAWQTVIIAVVVGAGIGL
                     AYSSLPALIIGAVDASETGAANGLNTLMRSIGTSVSSAVIGMVLAHMSKSVGPVTVPTM
                     AGFRVSFLIATAAVLIGVALASFLPSQRKASRPTLVAQSTDGEAADAADTDNARGAARP
                     VAETAAPLAATLPAPADPPAPAEPAAVAEAAEPTELTASAGQLAPLVKPAPSWAQPVTE
                     RHTAPSRPAPDATTAVAGAVAVAAGFRGRVVDTSGSPVSGASITLIDRQGRQAGVTTAD
                     ADGRYALDAPSAGTYVLTGAAPGHTPYAASATDHGKGGSTRVDLILAPTGRLGGTLLGG
                     AEGTPLAGGSIVVTDAEGAVVTRTTSGAEGSWHVPQLPPGHYTLVLSAVGHQPQARAME
                     LSGGAPERQDARLQPMATVRGTVRGPGGRPLTDAAVTLVEDGTVAGHTVTGPDGAFAFS
                     DLRGRHYTLTAAGYPPHSAPISLTAGASETLDLELAQRPTAAD"
     gene            complement(10306..10839)
                     /locus_tag="DKG71_RS39830"
                     /old_locus_tag="DKG71_39830"
     CDS             complement(10306..10839)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007268027.1"
                     /locus_tag="DKG71_RS39830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_39830"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_018092048.1"
                     /transl_table=11
                     /translation="MTEIRTPRLILRRWTDDDLAPMADINADPLVMRWIGDGSTLDLDR
                     TAEEIERWEEEWDDEGFGLFAVELLASGELAGCAGLSVPDFLPEARHEIAISWRLGRQY
                     WGQGYASEAAQAVLEFALQDRGLDRVIAINRLGHESSQNVVRKLGMAFEREATHPEYGH
                     ALTVHSIDLTEYQG"
ORIGIN
        1 ggcgggcttg caggccgcgc gtcaggcgca ggcggcgccg gtgggcgggc gcaccgcgac
       61 ggcggagggg tgacgctcac ggcggctgcc gaaatgcgcg agcgcggagc ccagcgcacg
      121 ctggaggcat gaccgagcat ccgcctgtca tcgtgcaccc gccgacgccg tccggcggcc
      181 gacgggtgac cgtgcgtggg cagatcgtcg gcctggccca tggccgcggc gatgttgctg
      241 aattcctccg ccgcgcgggc gtcgccagtc cggccgggga catcgagctg gacgaccccc
      301 gcctcgtcga atggcgcggt ggctcgctgg acgactggcc gatgccaccg ccttgaacgg
      361 cgcggccggt cggctgttgg ggcggcttcc gcgtcagcct gggaacgacg cgtacgcacg
      421 ccatcgtcag ccgtggccgt accgtgccgt agtcgggcct tctccgcatt cgtagccgcc
      481 gctcggtgcc gctgggcagg gggatacgct gacgagcgac gatgggcagg ccgtcggccg
      541 gtgagctgac ggcaggagag cgggagcacg acgtgagcga cgacgggacc agcgatgacg
      601 gcgggtactc gccgctgcgg ggcgggtcgg ggggttcttc ggacttgaac gtcctgcgga
      661 gggcgatgcg ctgggagacc ttcgcgctgg tcgcgttcct ggtgatcctt gcgatagtgg
      721 cgtcgtaggg ccggttcggc gcggcgaaac tcctcaattc gggcatatct ccctgggggt
      781 ccacgccgga gcgtccagcc ggacgcccgc acgagactct ctccccgggg gccgtaagcc
      841 tgaactcccg tactgctaaa ggctgttgtc tgccggtccg tcggtgtgcc gcacggtcgc
      901 gccgccctcc cgcggcagat ccgcgtctac gagccgggta ccccagcccg cacgcccgct
      961 gcctccccca agactgtgcg gccccgccgc accgccccgc cgcaccgccc ggccccacgg
     1021 cgcccccccc cacgccccga acgctccgtc cgatttccgc cagcgccgca gcctgacctg
     1081 ctgcacagtg gactctgtga tgaacgagga cagcaggtac gaggcggtgc gcagcaggga
     1141 cgccagattc gacggcgcct tcttcttcgc ggtgtccacg accggtatct actgccggcc
     1201 gagctgtccc gccaccacgc cccgacgccg gaatgtcgcc ttcttcccga cctcggcggc
     1261 cgcccagggg tccggcttcc gtgcctgccg gcgctgccgc cccgatgcgg tgcccggctc
     1321 gccggagtgg aatgcccgtg ccgatctcgt cggacgggcg atgcggctga tcagcgaggg
     1381 tgtggtcgac cgggaaggcg tctgcggtct ggccgcccgg ctcggctaca gcgcccggca
     1441 ggtccagcgc cagctgaacg ccgagctggg cgccgggccc gcggccctgg cgcgggcaca
     1501 gcgtgcccac accgcccggg tcctgctgca gaccaccgga ctgcaggcgg ccgagatcgc
     1561 cttcgcggcg ggcttcgcca gcgtgcggca gttcaacgac accatcaagg agatctacgc
     1621 cctcaccccg agcgggctgc gcgccgcccg ccccggcaaa ggcacccgat tcggaccggt
     1681 cgcgccgccc accactcccg gggtgctgcc gctgcggctg gccttccgcg ggccgtatgc
     1741 ggcgcggcag ctcttcgacc acctccagcg gcgcgcgatc accggtgtcg aggaggtcag
     1801 cggcgagccc ggcgcccgta cctaccggcg caccctgcgg ctgccgcacg gaaccgggat
     1861 cgccgaggtc gacgaggcgg ccggcgacgg ctggctggac tgccggctgc gcctgaccga
     1921 gctgcgggac ctcaccaccg cgagccagcg ggtgcgccgc ctcttcgatc tggacgccga
     1981 tccgtacgcg gtcgccgagc ggctcggtgc ggactccgta ctggcgcggc tggtggacgg
     2041 ctgccccgga ctgcgctccc cgggcgctcc cgccccggac gagctcgccg tccgtgccgt
     2101 actcggccag caggtctcgg tcgcggccgg ccgtacgctc ggcggcgccc tggtcgccgc
     2161 ccacggagaa ccgctgcccg agccggccgg tgcgctcacc cacctcttcc cccgcatcga
     2221 ggacctggcg caggccaccc tcaccgagct cgggatgccc gagtcgcggc gggcgaccct
     2281 gcgtactctg agcgccgcac tcgccgacgg cacggtcgtc ctggacgccg gagccgaccg
     2341 tgacgaggcc gaacgcgcgc tcctcgggct gcgcggcatc ggtccgtgga ccgccggata
     2401 catccggatg cgtgccctcg gggaccctga tgtcctgctg accggcgatg cggccgtact
     2461 ggccggcatg cgccgggccg gagtcccggc ggcaggcctg aaggagcggg cgacatcctg
     2521 gcgcccctgg cgctcgtacg cgatgcacca cttctggaac gcgccggcca cggagccggc
     2581 gaacccccgc cccaccacca cggaaagcac cggaccatga cgctctacac cacccttgac
     2641 agtccgctgg gcgaactcct gctggtgggc gaggaatcgg ccaccgcgcc gggcggcctc
     2701 gcgctcgcct cgctgtccgt gcccggacag aagggcgggg cgaccgtcca ggacggctgg
     2761 acgtacgccc ccgccgcctt cgccgacatc gcccaccagc tccgcgccta cttcgacggg
     2821 aagctgaccc gcttcggcat cgcctacgcc cccgcccggg gaacggactt ccagcggcgg
     2881 gtgtggcagg cgctggaggc catcccgtac ggcaccacca ccacgtacgg gaagatcgcc
     2941 gccgacatcg gtgcggcgcg gggtgcggtg cgcgccatcg gaacggcgat cggcgcgaac
     3001 ccgctgctcg tcgtccggcc ctgccaccgt gtgatcgccg cggacggttc cctttcgggt
     3061 tacgcgggcg ggcccgtacg caaacggcag ctcctcggca tcgagggcgc cctccccgat
     3121 gtcatgccgg atgccctctg agccaccccg cgccgcccgc gatcgccggg cgccgccccg
     3181 ccccgctgga gcgctccatg accaccacga ccactgccac caccacgacc accaagcgcc
     3241 acgcccgccc cgccacgctt gcccagcgcg tcgccgccgc cgactggacg gcgctggccg
     3301 atgaactcaa cactgtgggc tgcgcggtga ccccacgtct gctcagcccg gcggactgcc
     3361 gcgccctcag cgcgctgtac gacgcgccgg ccccggacca cggcgcggaa tccggcagcc
     3421 gtgcccgctt ccgctcgacc atcgacatgg cacggcaccg tttcggctcg ggccagtacc
     3481 gctacttcgc cgacccgctc cccgaaacgg ttcaggcact gcgcgaggcg ttctacccgc
     3541 acctgctgaa gaccgcccgc gactgggcgg acaagctcgg cagaccggcc ccctggccgg
     3601 acaccctgga ggagtggctg accctgtgcc acgaagccgg gcagaccaag tccgcacaga
     3661 ttctgctgcg ttacggggag ggcgactgga acgcgctgca ccgggacatc ttcggggaga
     3721 tggtcttccc cctccaggtg gtcatcggcc tggatgagca gggcaccgac tacaccggtg
     3781 gggaattcct cctcgtcgag cagcggccgc gcgcccagtc ccgcggcacc accaccgtcc
     3841 tgccgcaggg ccacggcctg atcttcacca cccgcgaccg ccccgtacgc tccgcccgag
     3901 gctggtcggc cggccccgta cgccacggtg tgagcaccgt ccgctccggc cggcggcact
     3961 ccctgggcct ggtattccac gacgcctgac gacgccgtcg cccgcgccgg cccagggcgc
     4021 tgcccgacgg cgtcagagca tggagccgcc ggtgacgtcg aagcgctggc cggtgatcca
     4081 gcgggagtcg tccgaggcga ggaaggcggc gacgtcggcg atgtcctggg gcttgccgat
     4141 ccggttgaac acggagaggg aggccagcgc ggcgtgcgcc tccggcgtcg cccgcatcgc
     4201 cgcgttcata tcggtctcga tgaagccggg ggcgatggtg ttcaccgtga tgccccgcgc
     4261 accgagatcc ttcgccaagg tgagggtgaa ggtgtccacc gcgcccttgg agacgccgta
     4321 ggcgatggtc gacgggaacg ccgtactcgt caccgccgac gagacgttga tgatgcgtcc
     4381 gccgtcccgc atccggcgca gaccgtgctg ggcgatgaag aacggggcct tggtgttgac
     4441 cgcgagggtg cggtcgaagt cctcgggtgt gacctcgtcg atgtgccccg ggatgctgat
     4501 cgccgcgttg ttgacaagga tgtcgagacc gggctcggcg ccgtacgccc tcaggccgtc
     4561 gtcgaacgcc gcgtagaggg cctcggcatc gccgggaacc cccagttccg caccgactat
     4621 gaaggcccgg ccgcccgccc tgatgatgct ctcggccgtt tccttggccg cggtgtcatt
     4681 actgccgtag tggacggcga ccagtgcgcc gtcggcggcg aggcgctgcg cgatgccccg
     4741 gccgatgccc cggctggatc ccgtcaccag tgcggtcttt cccttgagat cactcatgtg
     4801 gtccggcctt tcacgtgcct gtggtgggca taccgccccc ggaggactgt cggttggtgt
     4861 gtggtggtgc cgctgcaccg ggtcggctgc gcatgcctca agagccgctc ccgcacgggc
     4921 aggtggccgc ccgggactgg cgcggaggct cagaagaatg cgccggccag gggagaactg
     4981 cgtgccgtat gagcgggacg tcagtcccgt gggtccagcg ccgtcagcgt ggagacgaag
     5041 accggatcgc cgtcctgtag gccggtcacc tgcaccgtgg tggtgcaggg cagcgccgac
     5101 gggacacggc gggcttcgat cacacagggg gtgccgtgct cggcgtaccg gcggaaaacg
     5161 gtgccgaagg cgtacgggac gaaggcatcg ggcgaggtca ggacgcgtgc ggcctgctgc
     5221 gccgcgtcga gcagcgccat cccgggaatg tgatcgttcg ccggattcac caccgcgggg
     5281 tggcctggcg cgatgcgcaa cagccagcgg tcggtccggc cggtggggga gagcaggacg
     5341 tcgctgtcct tggcgctgcc ggtcagccgg ggtggaacgg gggcgggaag ggggtcgaaa
     5401 gtgcgccgcg cggtcagccg tttgccgcgg atccggcggt agacggcggg tgaggtgacc
     5461 gtgacgtcgg agtgaccggt cgcgacgatc cggcccgctt tgcggatggt catgtcgaag
     5521 tggccgctgg tcatcatgcc ggaccgcctg cgcatcctgc tgcaggcgac gtcgatggtc
     5581 agcggagtgg gcccgctgcc caccgcgaga tgctcggggt gtgtggtgta cacgaggtcg
     5641 ccgaggatga agtggtggcc gagtggtacg ccgagctcgg tgtgacagag cagcaacccg
     5701 gcctggcgga tcgtctcacc cgtgaggatg agatggtgtc ggccgcggtt gggggtgaaa
     5761 tacccgtgat cgtgcggcca ctgggcggtc agcgagaaat gcgcgtcatc gagccggcgc
     5821 catgaggtga ccagaatgtc gtcggcgagg gggcggtgta cgaactcccg agtgacctgg
     5881 acgctgcggc ggctccgcgg tgtggcggcc cggtccccgt tgaggtgcag ctgatgtgga
     5941 gacctgggtt gaagagtgcc gctgacctgc atcgtgtcgc tcatgactgc ccccaggcgt
     6001 cgctacgggt tgaggtacat cgctgataag atacgtacca tccggtttta ttttcaatgc
     6061 cgggcctgcg aaaaggggaa agtggctgat ccagcggcga cttgagctgt aactaactgt
     6121 tgcggaacgt ggatacatca agagacagga ggcgtcctgt ggcacaacag gaccgcgcga
     6181 ttcggacccg gcgagtgatc ttggaggcgg ccgcgtcggt tttcgacgag caaggctacg
     6241 accgcgcgac gatcgccgaa gtcctcgagc gtgccggcgt gacgaagggc gccttgtact
     6301 tccacttcgc ctccaaggag cagttggcgc tcgcggtgct cgaggagcag gtggtcgata
     6361 tcgccgtgga gccgcagaag atcaagctgc aggaattcgt cgactcgggc caggtgctgg
     6421 ccttccggct gcgcagagat ccgatccagc ggggtgcggc gcgactggcg gtcgagcagg
     6481 gttccaacca tctcgaccgc aagcagtcga tgctgtcgtg gacccggttc gtcgagggtt
     6541 tgctgaacga ggcccgggac cgcggggaaa tcctggagag catcgtcgtg cgggagaccg
     6601 ccgagctgtt cgtcggcgcg ttcgccgggc tgcagatgat gtcgcaggcc ctgaccaacc
     6661 aggccgacct cagtcgcagg ctgactgtct tctttgagca caccctcccc agcattgcgg
     6721 tgccggccat cctcgccaaa ctcaacctgg actctgagcg gggtgcgcaa ctcgacgccg
     6781 ccctgcaggg aaaggcgttg gcgaaggccg tggccggtgc cgatgccgaa gtggccgcgg
     6841 gctgagcccg tccgtggtgt gcaggtctcg ggccgcgaga ggcggatgac gtctcggcgc
     6901 attcagggcg ctcaaaacat accgcgcgcg cggtgttgaa tgcggaagtg tgtgcacctc
     6961 gtgtaagagt cgtgcaggtc aggggcttcg ccggaagcgc cgcgcgccgt tcgccgctga
     7021 ggtgacggcg atgcgcgggt gccgtgagcg gtgtgggcgg gtgtgcgggg atcgggtgcg
     7081 agcgggaatg tcagtggtgt gctgttcact ggattcggcg ggctcggagg atccgcgttc
     7141 agggcgagca ggggaggtcc ggtgcacggg gcgggggaga gtgcagggtg gggctgcgtg
     7201 aggcagatca cgggcgtcgg ggcgagaagt cgagccgtgc ggcggaatag gacgccgggg
     7261 tagaagcgtt cgtcatgcac gtatcagccg tgcgcggaga acgctccact tgtcagcggc
     7321 ctgatcgtgc cgatgcccgc agacgacacc acacttcgac cgctccttcg acgacgcctt
     7381 caccgccctc gggctgtgac gcccttcgcg gtccgacgac gctcacgccc ctaggtgcct
     7441 gccgccggcc cctgccgctc tctcaccttg cttcgcccct gacgcccccg acccacctct
     7501 gccgccctgg aggccgcgcg tatgaaccgg acgacgacgg atcagcttcc cgaaaggacc
     7561 gtgggcaccc cagccgtgcc cccggtgtcc gacccggcag tgggtgggaa gcgcagatcc
     7621 tccggcggcg tcgtcccggt cctcgccttc tccggcattg tggtcgcggt gatgcagacg
     7681 ctgctcgtgc cggtcatcaa ggacctgccg gtgctgctca acacgacggc cggcaacgcc
     7741 acttgggtca tgacggccac cctcctcgcc ggcgccgtct cgactcccat catggggcgc
     7801 ctcggtgacc tgtacggcaa gcggcggatg ctgctgacca gcctcgcggt catggtcgtc
     7861 ggctcgctga tctgcggctt caccagtgac ctggtcacca tgatcgtggg ccgggcgctg
     7921 cagggcttcg ccatgggcgc gattccgctc ggcataggca tcatgcgcga cgagctgccg
     7981 cgcgaacggc tcggctcggc catgggtctg atgagctcat cgatcggcgt cggcggcggg
     8041 ctcgcactgc cggccgcggc cctggtcgcc caacacgccg actggcacgc cctgttcttc
     8101 ggcgccgccg ggctcggcgt gctgtcgatg ctgctcaccc tcctcgtcgt accggagacc
     8161 gcggtacggg cccaggggcg gttcgacgtg accggcgcca tcgggctgtc ggccggtttg
     8221 gtggctctgc tgctgcccct caccaagggc agtgactggg gctggggttc gccgaccacc
     8281 ctcgtactct tcggtgttgc ggtcttgatc ctggtcctgt ggggcgtgat ggagctgcgt
     8341 ctcgccgatc cgctggtcga cctgcggacc accgcacgtc gtgaggtgct gctgaccaac
     8401 ctcgcatcga tcacggtcgg tgtggcgttc tatgcgatct ccctggtgct gccgcagctg
     8461 ctgcaactgt ccacgtcgac cggctacggc ctgggccagt cgatggtggt ggccgggctg
     8521 tgcatggcgc cgctgggcct gacgatgatg ttcgtcgcgc cgctgtacgc gcggatcgcc
     8581 gcgcggcgcg gccccaaggt ctcgctgctg ctcggcatgc tggtcatcgc gatcggctac
     8641 ggcgcgggca tcggcctgat gaatgcggcc tggcagaccg tcatcatcgc ggtggtcgtg
     8701 ggcgccggga taggcctggc ctactcctcg ctgcccgcgt tgatcatcgg tgccgtcgac
     8761 gcctcggaga ccggcgccgc caacggtctg aacaccctga tgcgctccat cggcacctcg
     8821 gtgtcgagcg cggtgatcgg catggtgctg gcccatatgt ccaagagcgt gggaccggtc
     8881 acggtgccca cgatggccgg cttccgggtc tctttcctca ttgccactgc cgcggttctc
     8941 atcggcgtag ccctggcgtc cttcctgccg tcacagcgga aggcgtcccg cccgaccctg
     9001 gtcgcgcaga gcaccgacgg agaggcggcg gacgcagccg acaccgacaa tgcgcggggt
     9061 gcggcgcggc cggtggcgga gaccgctgcg ccgctcgccg ccacgctccc cgcacctgcc
     9121 gacccgcccg cacctgctga accggccgca gtggctgaag cagccgaacc gaccgaactg
     9181 actgcatcgg ctgggcagtt ggccccgctg gtgaagccgg cgccgtcctg ggcgcagccg
     9241 gtcaccgaac ggcacacggc cccctcccgg ccggcccccg acgcgacgac tgccgttgcc
     9301 ggagccgttg ccgtcgccgc cggcttccgg ggccgggtgg tggacacctc cggcagtccg
     9361 gtctccggcg cgagcatcac cctgatcgac cggcagggcc gccaggccgg cgtcaccacg
     9421 gccgacgccg acggccgcta cgcactcgac gcaccctcgg ccggcaccta cgtcctcacg
     9481 ggggccgcgc ccggccacac cccgtacgcg gcctccgcca cggaccacgg caagggcggt
     9541 tcgacccggg tcgacctgat cctcgcaccc accggccgac tgggcggcac cttgctcggc
     9601 ggcgccgagg gcaccccgct ggccggcggg agcatcgtcg tcaccgatgc cgaaggcgcc
     9661 gtggtgaccc gtacgacatc cggtgccgag gggagttggc acgttcccca actgccgccc
     9721 ggccactaca ccctggtcct cagcgccgta ggtcatcagc cgcaggcccg cgccatggaa
     9781 ctgtccggcg gcgcaccgga acggcaggac gcccgtctcc agccgatggc tacggtccgc
     9841 ggcaccgtac ggggcccggg cggtcgacct ctcaccgacg ccgccgtgac cctcgtcgag
     9901 gacggcacgg tcgccggaca caccgtcacc ggaccggacg gcgccttcgc cttctccgac
     9961 ctgagaggcc ggcactacac cctcaccgcg gccggctacc cgccgcactc cgcccccata
    10021 tccctcaccg ccggcgccag cgaaaccctc gatctggagc tcgcccagcg accgacagcc
    10081 gccgactgag ctgctccggc ccaccaggca ccacagtcag ctgcccgccg gtccgctggc
    10141 ccgccgccgg tccggttccg gtgcgccgcg tggtgcttga ggggtcccat gggccggagg
    10201 gggggacgtc acggaacttc ggtggccgaa cccccggccc ccagcctccg gccccggcgc
    10261 tcgcccccca ggcgcccgac cccgtccccc cgaccccgcc ccccctcagc cctgatactc
    10321 cgtgaggtcg atgctgtgta ccgtcagtgc atggccgtac tccggatggg tcgcctcccg
    10381 ttcgaaggcc atgccgagct tgcgtacgac gttctgcgag gactcgtggc cgagccggtt
    10441 gatggcgatg acgcggtcga ggccgcggtc ctggagggcg aactccagca ccgcctgggc
    10501 ggcttcggag gcgtagccct ggccccagta ctgccggcct aggcgccagc tgatcgcgat
    10561 ctcgtgccgg gcctcgggaa ggaaatcggg gacggacagg ccggcgcatc cggccagttc
    10621 gcccgaggcg agcagttcga cggcgaagag gccgaagccc tcgtcgtccc actcctcctc
    10681 ccaccgttcg atttcctcgg cggtgcggtc caggtcgagg gtggagccgt cgccgatcca
    10741 gcgcatgacg agcgggtccg cgttgatgtc cgccatgggg gcgaggtcgt cgtcggtcca
    10801 gcggcggagg atgaggcggg gggtgcggat ctcggtcatg cctcccatcc tgtcgaatgc
    10861 gggggcgcgg cggtaatcgg cgggtgtccg ttcgcggtcc gtgcggtgca ggtcagcggc
    10921 tcagcagctg cgccagcgtc tcctcgagtt cgctctgcag gacgcggctc gccgcctccc
    10981 cgtc
//
