LOCUS       NZ_CP029541            11935 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. NEAU-S7GS2 chromosome, complete genome.
ACCESSION   NZ_CP029541
VERSION     NZ_CP029541.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NEAU-S7GS2
  ORGANISM  Streptomyces sp. NEAU-S7GS2
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Streptomyces sp. NEAU-S7GS2 isolated from saline-alkaline soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) School of Life Science, Northeast
            Agricultural University, Mucai Street, Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: AMRT Link v. v5.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003173275.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 05:28:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,458
            CDSs (total)                      :: 8,365
            Genes (coding)                    :: 8,052
            CDSs (with protein)               :: 8,052
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 313
            CDSs (without protein)            :: 313
            Pseudo Genes (ambiguous residues) :: 0 of 313
            Pseudo Genes (frameshifted)       :: 144 of 313
            Pseudo Genes (incomplete)         :: 230 of 313
            Pseudo Genes (internal stop)      :: 21 of 313
            Pseudo Genes (multiple problems)  :: 74 of 313
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:52:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8884796
            Orig. end                         :: 8896731
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11935
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="32"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11935
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11935
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6935](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6935
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    512..527
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.68"
                     /tool="antismash"
     gene            773..1150
                     /locus_tag="DKG71_RS38640"
                     /old_locus_tag="DKG71_38640"
     CDS             773..1150
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088738.1"
                     /locus_tag="DKG71_RS38640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_38640"
                     /product="DUF2267 domain-containing protein"
                     /protein_id="WP_109894915.1"
                     /transl_table=11
                     /translation="MDVKEFFQAVADRAGLSRQEAADLTRATLETLALRLSGGEVRDLA
                     LELPEPLRVSLQQGRGEMVIFGPDESIRRVRRRTGLSGPEADRGVRAVLSTLQEAISGK
                     EFGHAMSQLGKEYAELVETTP"
     misc_feature    complement(1304..1318)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.7"
                     /tool="antismash"
     misc_feature    1375..1390
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.3"
                     /tool="antismash"
     gene            1620..2891
                     /locus_tag="DKG71_RS38645"
                     /old_locus_tag="DKG71_38645"
     CDS             1620..2891
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088740.1"
                     /locus_tag="DKG71_RS38645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_38645"
                     /product="MFS transporter"
                     /protein_id="WP_093639292.1"
                     /transl_table=11
                     /translation="MTGSAPAVAPAAPLPHVRRDLRLFWWAGTCDALGSQTSGLVLPLL
                     LLTLGRSPAEVGALAGLSAAATLLLGPFAAVPADRGARKHLMVGSALVSAAAMAGVTVA
                     VAAGPVPLAVLVAAVLVERCATSCYEAAELGTVALVCPPAEHRRVLSRLQAGERGALVL
                     GPALGGLLFHAERWLPFLADALSYAVAAGCIAAMRSPLVPPPAQRPPAGTVHRGLRARA
                     AEAGAGLALIRRDPFLRLALVWISAVNALLVALYYTTVFALQNDGRGAAPLGLVLALAG
                     AAGLAGALAAPRIAGRRPTGRVLVTVSWLMVPPAAALATARDAWHFGLLFGLLCLLTPA
                     ATVALQARIIQVIPPGLQARTGTVLATVSGAAAAVAPALAGLSADRAGAAATLVGCAAL
                     LAGLALYTTCAAPARTRHAATEPV"
     misc_feature    2076..2078
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2888..4738
                     /locus_tag="DKG71_RS38650"
                     /old_locus_tag="DKG71_38650"
     CDS             2888..4738
                     /NRPS_PKS="Domain: Abhydrolase_1 (363-504). E-value:
                     1.4e-09. Score: 29.9. Matches aSDomain:
                     nrpspksdomains_DKG71_RS38650_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088741.1"
                     /locus_tag="DKG71_RS38650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_38650"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_109894916.1"
                     /sec_met_domain="PF00561 (E-value: 6.8e-06, bitscore: 24.8,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 2e-35, bitscore:
                     121.1, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSMTAAHPAALPPSGTGFRVYHPALPEVCPRDPARMALAADADGR
                     CEIFTWNAATGTARQVTDSPHGTLHCALDAEARVWWFAEDRSGLGLWYFQDFEGGARQP
                     GLTGLPPGFPHGLAVSDAGTVAIGLGDGRGMTVHLGRPGGPARQVWTVDGQARLAGISP
                     SGGLLALSGAAGSDRAVTLLTCSGAVLDQLSGHHGRLWALGFAPSPAGTELLLVQEHHD
                     HYRLATWRPGSGLLPQDWCRFDTEITARWYPAGRRVLIRQDRHGRSTLAAADLDRRTLT
                     PVPSPPGTLLDAAPHQGDEVHYLWTDTATPPRMRSTAGTRLPELATLPAPTPGRHRELW
                     TPGPDGPVHTLLSTPDPTTGRRPPLVFLVHGGPADHDRDAYDPEVHSLVASGFAVARVN
                     YRGSTGYGPRWRTAYGAGVGLTQVADLVAVRADLLRRGLARADATGLWGTSWGGYLALL
                     ALGTRPDLWQAGVAVKPVADCAAAYRTGTPALRALDERLFGGTPDAVPERYARSSPLHY
                     AAEVRAPLLVIAATLDAKCPPGQVRSYLDALRQAGAAHESMWLETGHDGYTGAHHVAVL
                     HRAMGFLDQHLRRAPAAHRTPRPSRDGVSGGTGPSGAPGS"
     aSDomain        3977..4399
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DKG71_RS38650_Abhydrolase_1.1"
                     /evalue="1.40E-09"
                     /label="DKG71_RS38650_Abhydrolase_1.1"
                     /locus_tag="DKG71_RS38650"
                     /protein_end="504"
                     /protein_start="363"
                     /score="29.9"
                     /tool="antismash"
                     /translation="PLVFLVHGGPADHDRDAYDPEVHSLVASGFAVARVNYRGSTGYGP
                     RWRTAYGAGVGLTQVADLVAVRADLLRRGLARADATGLWGTSWGGYLALLALGTRPDLW
                     QAGVAVKPVADCAAAYRTGTPALRALDERLFGGTPDA"
     gene            5001..6935
                     /locus_tag="DKG71_RS38655"
                     /old_locus_tag="DKG71_38655"
     CDS             5001..6935
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088743.1"
                     /locus_tag="DKG71_RS38655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_38655"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_109894918.1"
                     /sec_met_domain="TIGR03975 (E-value: 1.4e-180, bitscore:
                     600.6, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILRRSVDERTSGRADVLHANLEFT
                     DWITRRTAFTADDYQFYALSSYFMGCGDWVFSSALYDDPEWRVPEFSVSMRGKLRDERL
                     RMSKELHRVVPEFVQEIAERIVAAGPDVVGFTSTFQQNTAALAAVKYVKRLAPHIKTVM
                     GGANCDAEQGAATHRNFPFLDFVVRGEGEAAFPQLLTALDEGGDLSAVPGLCHWGADGK
                     SIANPMSTRPLPPATILPPDYSGYFERLASSVARNWVEPKLVVEGARGCWWGEKHHCTF
                     CGLNGSFMQFRSKSPDTFYEEIMELARRHRVLDMYLVDNILDMGYLTTVLPRIIDSGYD
                     LRMHIEIKANMRRTQLRTLAEAGMIYVQPGIESLNSRVLDLMDKGVSGCQNVRMLRDGA
                     ETGLSVSWNYLHGFPGESAADYEPVIAQIPALEHLDPPVDLSARIAIERFSPYFNRPEL
                     GFTGLRPEEHYRFTYDLPESELLDMAYVFEAPERGITEPTVTALNGALGAWKKHHADSR
                     LTHADLGDRIVLVSRRRAFNWGAMELTAPTEIAAFRLLDQPHAPAALTRKLTARLPEHP
                     VDEAAVHALLQHWVTLGLVFTDGGQYVHLAPAAVNEDLLRLDFMRHTHAATTPQEEPDD
                     AAHALVAHV"
     gene            6985..7659
                     /locus_tag="DKG71_RS38660"
                     /old_locus_tag="DKG71_38660"
     CDS             6985..7659
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088744.1"
                     /locus_tag="DKG71_RS38660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_38660"
                     /product="DUF5825 family protein"
                     /protein_id="WP_109894920.1"
                     /transl_table=11
                     /translation="MTSTAAPASRPLTLHAWRDYDEDACTLPGMSLGAVDLTAPPHDQA
                     AELWELGARRVEFTGEIDLTAVDDPADAAHAVRRLCLIRDLTARAVLVQWQLRLPPEPD
                     DGWRDLSHLQPPRTLTGPADPVAALAQWRNEHYLCKCLWRQGPGFVQIRDRRWGELRRF
                     TAEEPEYQEAITQLSYGTPLRSVPRAIAADFLEERLVSRTGSLLWWLPYRVNRWTQEAM
                     AI"
     gene            complement(7735..8793)
                     /locus_tag="DKG71_RS38665"
                     /old_locus_tag="DKG71_38665"
     CDS             complement(7735..8793)
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /locus_tag="DKG71_RS38665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_38665"
                     /product="RICIN domain-containing protein"
                     /protein_id="WP_109894922.1"
                     /transl_table=11
                     /translation="MKPPRRSGLRRTFAVLITALAVTLGLSAQSGTARADDGSGLSGLT
                     FTSVNNGRNLDVQNGNTGDGVFIVTNSAPGHHQKWSANAQPDGSFTLVNDATGKCIEAG
                     HPLKQRSCSGASGLRWYFQPVNGTSDTYLIRNAGDNKCIDVVLGAQYDDAWTQAYGCNG
                     SKAQQWKIPSSATGAAFKAAVAYASKRCQKDAATCSWTKGTQAPAEPLPKQCISPVWYN
                     GTDSPVPWTFTLNTSSGWSSQLGVSFTSTLGTGMASPVQTSVSLTLSGQVTYDLRTDLG
                     NSLTINVPSRHYGWVALSELATKVTGEWTFDANGFPWKAKDTVTVPLRSDDQGRSSIYL
                     AQTSPDFTTCNS"
     misc_feature    complement(8767..8769)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(8958..9905)
                     /locus_tag="DKG71_RS38670"
                     /old_locus_tag="DKG71_38670"
     CDS             complement(8958..9905)
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088746.1"
                     /locus_tag="DKG71_RS38670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_38670"
                     /product="DMT family transporter"
                     /protein_id="WP_109894924.1"
                     /transl_table=11
                     /translation="MSRADGRALAAASVTVVLWASSFIAIRSSAADFGPGALALGRLAV
                     ASVVLSVLLLVRRGGFPPREAWPGIIASGVLWFGAYMVALNWGEKLVDAGTASLVVNVG
                     PILMALLGGWLLKEGFPPRLMAGMAVSFAGAVVVGLSMSGGGKSSLLGVLLCLFAALAY
                     AVGVVLQKPTLKHATPMQVTTFGALAGTVACLPFAGQLVSQVSRAPVSATLQMVYLGVF
                     PTALAFTTWAYAVSRTTVGKMGTTTYAVPVLVVLMSWLFLGEVPGWLTLLGGLLCLSGV
                     AVSRMRGKARAQVPDDARGSRAAAQPDVSADSAR"
     gene            complement(10170..10505)
                     /locus_tag="DKG71_RS38675"
                     /old_locus_tag="DKG71_38675"
     CDS             complement(10170..10505)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004943836.1"
                     /locus_tag="DKG71_RS38675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_38675"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_204166122.1"
                     /transl_table=11
                     /translation="MLQILGLVGSKWSILIVGQLRDRTMRFGELHRAVKGISQRMLTLT
                     LRQLERDGLLTRTVHPSVPPRVDYALTALGATLLDSVVALGEWASAHRHEINNNRHRYD
                     ATHPTRT"
     misc_feature    complement(10669..10678)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.26"
                     /tool="antismash"
     gene            10761..11474
                     /locus_tag="DKG71_RS38680"
                     /old_locus_tag="DKG71_38680"
     CDS             10761..11474
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004943834.1"
                     /locus_tag="DKG71_RS38680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_38680"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_109894928.1"
                     /transl_table=11
                     /translation="MSKTLVVVGAGPGLGMGVARAFGLQGFRVGLIARTESKLHTLVAE
                     LADLGITAAAFPADIRDRDALTTALAAVADTLGPIDVLEYSPSPTGPITHAARTTVDSV
                     TAQFELHVLGAVTAVGHVLPQMLARGDGTLLLTTGVSSTIPAPFLANVGMAMAGLRNWA
                     HTLHAELKPQGIHVGTVTIASGITPGGEADPDAIGARYYSMYQQRNRIEEVIGDLDAFR
                     SLVTRTGGHGTPVGV"
     gene            complement(11482..11814)
                     /locus_tag="DKG71_RS38685"
                     /old_locus_tag="DKG71_38685"
     CDS             complement(11482..11814)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088749.1"
                     /locus_tag="DKG71_RS38685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_38685"
                     /product="cupin domain-containing protein"
                     /protein_id="WP_109894930.1"
                     /transl_table=11
                     /translation="MSETTETGPLKTAVQVDEVAPVEVVPGIVRRRLPRTAYAGGWLID
                     FAPGAEWPDVDVHATEERYYVLSGEVIEGEERHGPGTYVVFAPGSRHRPRTEMGARMLG
                     ITMLPR"
ORIGIN
        1 ccggtggaaa gaaggaccgc ggcgccggtc tgctgtccgg cgagggattc ggcggaggca
       61 attcgcccga cgtcatcacc gtccgcaccg agtaccccta tcagaccctc caagcggttc
      121 ccgagaactc cgaactcccg gcattctccc tctgggcggc ctacgtaccg aaggacgcgg
      181 agcagcgcac ccatcatgcc tatggcctgc tcatgattgc gaaaccgccg atcccgggag
      241 ccctgaatct cgcctggccg ctcatccggc gcttcaccga gcgggtgttc gccgaggacc
      301 ggatggcggt cgaggccgag cagcgggcct gggacgagca gggcgaggac tggaaccatg
      361 aggtcttccc gctgatcctc gatgtccgtg acgtgttgcg caccaacggc gtccccatcc
      421 gccccgaatc cgccctctgc ggtgccgccg cgttctgcga cggcgcaccg cgggccggca
      481 caccatcgga caccgcctcg caccagccgg gctgaccggc tggtgcgaca tgggggggga
      541 gggttccggc tcgcgcggaa ggcgcttgac ccgtcctcac tccaggtcgg acgccttccg
      601 gcgggcgcgg ccgcgaaccg cccgtgacct ggtgactttc ggttgccgcg accgtgcagg
      661 gggtggcggg tggcgggtcg agcgtgggcc gacggtgggc gggggatcgt ggcaaggtgg
      721 tgagtgaggc tgcctccctc gttcatccct cgattccgtt gtggaggctc gcatggacgt
      781 caaggagttc ttccaggcag tggcggatcg cgcggggctc tcacgacagg aagcggccga
      841 tctcacccgc gccacccttg agacgctggc gctgcggctg agcggcggcg aagttcgcga
      901 tctggccctc gaacttcccg agccgctgcg tgtctcgctc cagcaggggc ggggagagat
      961 ggtgatcttc gggccggatg agtcgatccg cagggtgcgt cggcgcaccg gtctgtccgg
     1021 gccggaagcc gaccgcggtg tacgggcggt cctcagcacg ctgcaagagg ccatctccgg
     1081 caaggagttc gggcacgcca tgtcgcagct cggcaaggag tacgcagagc tggtggagac
     1141 cacgccctga cggcccttcc ctgacggccc ttccctgacg ggcctgctcg tccggcgatc
     1201 tctacggcct gctcaccgcg gctgacgcga agaggtgagg gtggcggagt gatggtcgcg
     1261 ggggcgacgg cccgtccgaa cgaaccgctc gaaggtgttc tgcgcgcaca ccttgtggcc
     1321 cacgtcactg tctggctaag ctccggcacg attcgggcac atttcctccg caagttgacc
     1381 tattggtgag gcgatcttca cggtctgccc acatgtgtga ggtggcttcg catggcacgg
     1441 tcagcggcat ccccggcagg cggcgcaccc tacgacttat cgcgcgtctc ctcccgggac
     1501 gcgacggcga acggcccgac gtacggcccg gcggacggcc tgacgtacgg ccgcccggcc
     1561 cgcccggcgc acgacgacgc ccgccccggc accgcgagac cggccccgga ccgtgcctga
     1621 tgaccgggtc cgccccggcg gtggcaccgg ccgctccgtt gccccacgtc cgccgcgacc
     1681 tccggctgtt ctggtgggcg ggcacctgtg acgccctggg cagccagacg tccgggctcg
     1741 tcctgccgct gttgctgctg accctcggcc ggtcccccgc cgaggtcggc gccctcgccg
     1801 gactctccgc ggccgccacg ctcctgctcg gcccgttcgc cgccgtcccc gccgaccgcg
     1861 gggcgcgcaa gcacctgatg gtgggctccg cactggtctc cgccgccgcg atggccggcg
     1921 tcaccgtcgc cgtcgccgcg ggcccggtcc cgctggccgt gctcgtcgcc gcggtgctcg
     1981 tcgagcgctg cgcgacctcc tgctacgagg cggcggagct cgggaccgtc gcgctggtgt
     2041 gcccgcccgc ggagcaccgg cgcgtcctgt cccgattaca ggcgggggag cggggcgcac
     2101 tcgtcctcgg tcccgcactg ggcgggctgc tcttccacgc cgaacgctgg ctgcccttcc
     2161 tggcggacgc cctctcgtac gccgtcgcgg ccggctgtat cgccgccatg cgctcccccc
     2221 tcgtcccgcc ccctgcgcag cgtccgccgg ccggcacggt gcaccgcggt ctgcgggccc
     2281 gggccgccga agccggagcg ggactggcgc tgatccgccg cgaccccttc ctgcgactgg
     2341 cgctcgtgtg gatctcggcc gtcaacgccc tgctcgtcgc gctgtattac acgacggtct
     2401 tcgctcttca gaacgacggc cggggcgcgg cgccgctggg tctggtcctc gcgctcgccg
     2461 gggcggccgg cctggccggt gcgctcgccg caccccggat cgccggccgg cgccccacgg
     2521 gccgcgtgct ggtgacggtc tcctggctga tggtgccgcc ggccgctgcc ctggcgacgg
     2581 ctcgcgacgc ctggcacttc ggcctgctgt tcggcctgct gtgtctgctg acgccggccg
     2641 ccaccgtggc gctgcaggcc cggatcatcc aggtcatccc gcccggactc caggcccgca
     2701 ccggcaccgt actggccacc gtgtcgggcg cggccgcggc agtggcgccc gccctggccg
     2761 ggctgtcggc cgaccgggcc ggggccgccg ccaccctcgt gggctgcgcc gcgctgctgg
     2821 ccgggctcgc gctctacacg acctgtgcgg caccggcccg cacccgccac gccgctacgg
     2881 agcccgtatg agcatgaccg cggcacaccc cgccgccctg ccgccttccg gtaccggctt
     2941 ccgggtctac cacccggcgc tccccgaggt ctgcccgcgc gaccccgcgc ggatggcgct
     3001 ggccgcggac gccgacggcc gctgcgagat cttcacctgg aacgccgcca ccggcaccgc
     3061 acgccaggtc accgacagcc cgcacggcac cctgcactgc gccctcgacg ccgaggcccg
     3121 ggtgtggtgg ttcgcggagg accgcagcgg cctcgggctg tggtacttcc aggacttcga
     3181 gggcggtgcc cggcagccgg gcctgaccgg cctgccgccg ggcttcccgc acggcctcgc
     3241 cgtgagcgac gccgggaccg tggccatcgg actcggcgac ggccgcggga tgaccgtcca
     3301 cctcgggagg ccgggaggcc cggcccggca ggtgtggacc gtcgacgggc aggcgcggct
     3361 ggcgggcatc tcgccctcgg gagggctgct ggccctgtcc ggagcggccg gctccgaccg
     3421 ggcggtcacc ctcctgacct gctccggtgc ggtgctcgac cagctctccg gacaccacgg
     3481 ccgactgtgg gcgctcggct tcgcgccgtc cccggcaggc accgagctgc tgctggtcca
     3541 ggaacaccac gaccactacc ggctcgcgac gtggcggccc ggcagcgggc tgctgcccca
     3601 ggactggtgc cgcttcgaca ccgagatcac cgcgcgctgg taccccgcgg ggcgccgggt
     3661 cctcatccgc caggaccgcc acggccgttc caccctcgcc gcggccgacc tcgaccggcg
     3721 cacgctgacc ccggtgccct ccccgcccgg caccctgctc gacgccgccc cgcaccaggg
     3781 cgacgaggtg cactacctct ggaccgacac cgccaccccg ccccggatgc gctccaccgc
     3841 cggcacccgg ctcccggagc tcgccaccct gccagccccc acgcccgggc ggcaccgcga
     3901 actgtggacc cctggcccgg acggccccgt gcacaccttg ctcagcaccc cggacccgac
     3961 gaccggccgg cggccaccgc tggtgttcct ggtgcacggc ggccccgccg accacgaccg
     4021 ggacgcctac gacccggagg tgcactccct ggtcgcctcc gggttcgcgg tggcccgggt
     4081 caactaccgc ggctccaccg gttacggccc ccgctggcgc accgcgtacg gcgcgggcgt
     4141 cgggctcacc caggtcgccg acctcgtcgc ggtccgtgcc gatctgctcc gccgcgggct
     4201 ggcccgggcg gacgcgaccg gcctgtgggg cacctcctgg ggcggctacc tggccctgct
     4261 ggcgctcggc acccggccgg acctctggca ggcgggcgtc gccgtcaaac cggtcgccga
     4321 ctgcgcggcg gcgtaccgca ccggcacccc cgcactgcgg gccctggacg agcggctgtt
     4381 cggcggcacg cccgacgcgg tgcccgagcg ctacgcccgg agctcacccc tccactacgc
     4441 cgccgaggtc cgggccccgc tgctggtgat cgccgccacg ctcgacgcca aatgcccgcc
     4501 cggccaggta cggagctacc tcgacgcgct gcggcaggcc ggtgcggcac acgagtcgat
     4561 gtggctggag accggccacg acggctacac cggcgcccac cacgtggccg tgctgcaccg
     4621 cgccatgggc tttctggacc agcatctgcg ccgcgccccc gcggcccacc gaaccccccg
     4681 tccatcgagg gacggggtct ccggaggcac cggcccgtcc ggtgcccccg gatcgtgaga
     4741 tccgttccgc aaccggcaga agaggagcag cagccatgca gaaggacatc atccacaacg
     4801 acccgctcgc gggcgacgag gagaaccgca ggccgggcat cggcatcacg gtgaccgtcc
     4861 cgttccgtaa cgccgaggac aacgaggagg actgaccctc cccggccggc ccggttgcac
     4921 accgggccgg ccggacccgc gcacgtcacc ggccggccgc ccggccacct cccaccccac
     4981 ccgccgtacg aggagccgcc atgcgcgtcc tgctggtcaa tatgccctgg tccccgatcg
     5041 acctgccgtc cctcgccctc ggcatcctga gacgaagcgt cgacgagcgc acctccgggc
     5101 gggccgatgt cctgcacgcg aacctggagt tcaccgactg gatcacccgg cggaccgcct
     5161 tcaccgcgga cgactaccag ttctacgcgc tctcctcgta cttcatggga tgcggcgact
     5221 gggtgttctc ctccgccctg tacgacgatc ccgagtggcg ggtaccggag ttcagcgtct
     5281 cgatgcgcgg caagctgcgc gacgaacggc tgcggatgtc caaggaactg caccgcgtgg
     5341 taccggagtt cgtgcaggag atcgccgagc ggatcgtcgc ggccgggccc gacgtcgtcg
     5401 gcttcacctc caccttccag cagaacaccg ccgcgctcgc cgccgtcaag tacgtcaaac
     5461 gcctcgcccc gcacatcaaa accgtcatgg gcggcgccaa ctgcgacgcc gagcagggcg
     5521 ccgccaccca ccgcaacttc ccgttcctgg acttcgtggt ccgcggcgag ggagaggccg
     5581 ccttccccca gctgctcacc gctctcgacg agggcggcga cctgtccgcc gtccccggac
     5641 tgtgccattg gggcgccgac ggcaagagca tcgcgaaccc gatgagcacc cgcccgctgc
     5701 cgcccgccac catcctgccg cccgactaca gcgggtactt cgagcggctg gcgtcctccg
     5761 tcgcccgcaa ctgggtggag cccaaactcg tcgtcgaggg ggcccgcggc tgctggtggg
     5821 gggagaagca ccactgcacc ttctgcggtc tcaacggctc cttcatgcag ttccgcagca
     5881 agagccccga caccttctac gaagagatca tggagctggc ccgccggcac cgggtgctgg
     5941 acatgtacct cgtcgacaac atcctcgaca tgggctacct caccaccgtc ctgccccgca
     6001 tcatcgacag cggctacgac ctgcggatgc atatcgagat caaggcgaat atgcgccgta
     6061 cccagctgcg caccctggcc gaggccggaa tgatctacgt ccagccgggc atcgagagcc
     6121 tcaacagccg ggtgctcgac ctgatggaca agggcgtgag cggctgccag aacgtccgca
     6181 tgctccggga cggggccgag accgggctct ccgtctcctg gaactacctc cacggcttcc
     6241 ccggcgagag cgccgccgac tacgagcccg tcatcgccca gataccggcc ctggaacacc
     6301 tcgatccgcc ggtcgacctg tccgcccgta tcgccatcga acgcttcagc ccgtacttca
     6361 accggcccga gctcggcttc acgggtctgc gccccgagga gcactaccgc ttcacctatg
     6421 acctgcccga gtccgaactc ctcgatatgg cctatgtctt cgaggcacct gagcgcggca
     6481 tcaccgagcc caccgtcacc gcgctcaacg gcgccctcgg cgcctggaag aagcaccacg
     6541 cggacagcag gctcacccat gcggacctcg gcgaccgcat cgtgctcgtc agccgccggc
     6601 gcgccttcaa ctggggcgcc atggaactga ccgcgcccac cgagatcgcg gccttccggc
     6661 tcctcgacca gccgcacgcc cccgccgcgc tgacccgcaa gctcaccgcc cgcctgcccg
     6721 aacaccccgt cgacgaggcc gccgtccatg ccctcctcca gcactgggtg accctcggac
     6781 tggtcttcac cgacggcggc cagtacgtcc atctggcacc ggccgccgtc aacgaggacc
     6841 tgctgcggct cgacttcatg cgccacaccc acgccgcgac caccccgcag gaagagcccg
     6901 acgacgccgc ccacgccctc gtcgcccacg tctgagacac cgaccggccc gcagcaggac
     6961 aaccgtgccc cgcaggagaa acccatgacc agcaccgccg cccccgcctc ccggccactg
     7021 accctccacg cctggcggga ctacgacgag gacgcctgca ctctgccggg catgagcctg
     7081 ggtgccgtcg acctgaccgc cccgccgcac gatcaggccg ccgagctgtg ggaactcggg
     7141 gcacgccggg tggagttcac cggcgagatc gacctgaccg cggtcgacga cccggccgat
     7201 gccgcccacg ccgtccggcg gctgtgcctg atccgcgatc tgacggcccg tgccgtgctg
     7261 gtgcagtggc agctgcggct tcccccggag cccgacgacg gctggcgcga cctcagccac
     7321 ctccagccgc cccggaccct caccggcccc gccgaccccg tggccgcgct cgcccagtgg
     7381 cgcaacgagc actacctctg caagtgcctg tggcgccagg gccccggctt cgtccagatc
     7441 cgcgaccgcc gctggggcga actgcgccgc ttcaccgcag aggagcccga ataccaggag
     7501 gcgatcactc aactctccta cggaacacca ctacggtccg tcccgagggc catcgccgcc
     7561 gacttcctgg aggagcgcct ggtctcccgc accggctccc tgctgtggtg gctcccgtac
     7621 cgggtgaacc ggtggaccca ggaggcgatg gccatctgat gccggcaggg gagtggtgcg
     7681 ctccacaggg tgggcccgca cgacccacga ccccgtgcgg gcccaccttc tgactcagct
     7741 gttacacgtc gtgaagtccg ggctggtctg cgccagatag atgctggacc ggccctggtc
     7801 atcggaccgc agcggaacgg tgaccgtgtc cttcgccttc cacgggaagc cgttcgcatc
     7861 gaaggtccac tcaccggtca ccttcgtcgc cagctccgac agtgccaccc atccgtagtg
     7921 acgcgacggc acgttgatcg tcaggctgtt gccgagatcc gtccgcaggt cgtaggtgac
     7981 ctggccggag agggtgaggc tgacgctggt ctgcaccggg ctcgccatac ccgtgccgag
     8041 ggtcgaggtg aaggacaccc cgagctgact cgaccagccg ctggaggtgt tcagcgtgaa
     8101 cgtccacggc accggcgagt cggtgccgtt gtaccagacg ggagagatgc actgcttggg
     8161 cagcggctcg gccggggcct gcgtcccctt cgtccaggag caggtcgctg cgtccttctg
     8221 gcagcgcttc gacgcatagg ccaccgcggc cttgaacgcg gcgccggtcg ccgacgaggg
     8281 gatcttccac tgctgcgcct tgctgccgtt gcagccgtag gcctgtgtcc acgcgtcgtc
     8341 gtactgggcg cccaggacga cgtcgatgca cttgttgtcg ccggcgttac ggatcaggta
     8401 ggtgtcgctg gtgccgttca cgggctggaa gtaccagcgc agtccggacg ctccggaaca
     8461 ggaccgctgc ttgagcgggt ggccggcttc gatgcacttg ccggtggcgt cgttgacgag
     8521 ggtgaacgac ccgtcgggct gcgcgttggc gctccacttc tggtggtggc ccggcgcgga
     8581 gttggtgacg atgaagacac cgtcaccggt gttgccgttc tgtacgtcca ggttccggcc
     8641 gttgttgacg gacgtgaagg tcaggccgct caggcccgag ccgtcgtcgg cacgggccgt
     8701 gccggactgg gcggacaggc cgagggtgac ggcgagcgcg gtgatgagga cggcgaatgt
     8761 tctgcgtaag ccgctgcgtc tgggcggttt cataccagaa caaccccctt atctgatcgg
     8821 agtgttggtg gggtgttcca cctcgttccg cacgctatcg ctcccggtga tcctgcggca
     8881 gtactccgcc gcacgcgctg agagccgccc cggcccgcgg ccctcgctcg cgatccccgc
     8941 agtgccgcgt ccgccgccta ccgcgcagaa tccgccgaga cgtcgggctg ggccgccgcg
     9001 cggctcccac gcgcgtcgtc cgggacctgc gcccgcgcct tgccccgcat ccgcgacacc
     9061 gctaccccgg acaggcacag gaggccaccg aggagcgtca gccagccggg cacctcgccg
     9121 aggaacagcc acgacatgag gaccacgagc acgggcaccg cgtaggtcgt ggtacccatc
     9181 ttgccgacgg tcgtacggga caccgcgtag gcccacgtgg tgaacgccag ggctgtcggg
     9241 aagacgccca ggtacaccat ctgcaatgtc gccgacaccg gggcccggct cacctgcgac
     9301 accaactgtc cggcgaacgg cagacaggcc accgttccgg cgagcgcacc gaacgtggtc
     9361 acctgcatcg gagtggcgtg cttcagcgtc ggcttctgca gcaccacacc caccgcgtac
     9421 gccagcgccg cgaacaggca cagcagcacg ccgaggagag acgacttccc gccgccggac
     9481 atggacagcc cgaccaccac ggccccggcg aacgacacgg ccatgcccgc catcagcctg
     9541 ggcgggaagc cctccttgag cagccatccg cccaggagcg ccatcaggat cggcccgaca
     9601 ttgacgacca gggaagccgt tcccgcgtcc acgagcttct cgccccagtt caaggcgacc
     9661 atgtatgcgc cgaaccacag gactcccgag gcgatgatgc cgggccaggc ctcgcggggc
     9721 gggaatccac cgcgccggac cagcaggagg acgctcaaca cgacggacgc caccgccagc
     9781 ctgcccaggg cgagtgcgcc cggcccgaaa tccgcggcgg agctgcggat cgcgatgaac
     9841 gacgacgccc acagcaccac ggtgacggag gccgcggcga gcgcccggcc atccgccctg
     9901 ctcacggacc tggcgtcgcc atcccctgcc tgtgttccct ggctcactcc tgcaccctcc
     9961 cgagctcttt gcgtgcgccc tgcgcacgca catgagccgc cagcttgtaa gagccgatgc
    10021 ctgggtgaca agcgagtaag tgagctcgtg gggtggaccg accagctgct cctggccggt
    10081 ctcattggct ccggcgggtt gcgcacaacc agcggtgagg ggccggccga gcgccggaac
    10141 tgctcggcag catgaaccaa cgggcccgtt caggtccgtg tggggtgcgt ggcgtcgtag
    10201 cggtgccgat tgttgttgat ctcgtggcgg tgtgcggagg cccattctcc gagagcgacg
    10261 acggagtcca gcagcgtcgc gccgagtgcg gtgagggcgt agtccacgcg tgggggaacg
    10321 ctcgggtgga cggtgcgggt cagcaggccg tcgcgttcga gttgccgcag tgtcaaggtg
    10381 agcatgcgct gggagatgcc cttgacggcg cggtgcagct cgccgaaccg catggtgcgg
    10441 tcgcggagtt gcccgacgat caggatgctc cacttgctgc cgaccagccc gaggatctgg
    10501 agcacctccc ggcatgcttc ctcgtcggac tcgtggtcgc cgccggttgt cgggggcggt
    10561 gtggagtggt ggtgctgcgg gttcggggag gtgttcctgt ggggcgccaa tggttacctc
    10621 cgtgtgcgtc accgacatga atgtgccgtc ttgttgcgac ctttcccgta acaaatcatg
    10681 gggtcacgca cagacagtaa gtggtcggcg gcgggtcgcg gtaacaccgg tccgatcgct
    10741 gtgatcggaa ggaatccggg gtgtccaaaa cgctcgttgt ggtcggtgcc gggccggggc
    10801 tggggatggg ggtggcccgc gcattcggcc tccagggctt tcgggtcggg ctgatcgccc
    10861 gtaccgagag caagctgcac accctggtgg ctgagctggc cgacctgggt atcacggccg
    10921 ccgcgttccc agccgatatc cgcgatcgcg acgcgctcac cacagccctc gccgcggtgg
    10981 cggacacgct ggggccgatc gacgtgctcg aatacagccc cagcccgacc gggccgatca
    11041 cccacgccgc acggaccacg gtcgactccg tcaccgcgca gttcgagctg catgtgctgg
    11101 gcgcggtgac cgccgtgggc catgtgctgc cgcagatgct ggcccgaggc gacggcaccc
    11161 tactgctgac cactggtgtt tcctcgacga ttcccgcccc tttcctggcc aacgtgggta
    11221 tggccatggc cggcctgcgg aactgggcac acaccctgca cgctgaactc aagccacagg
    11281 gcatccacgt ggggacggtg accatcgcct ccgggatcac ccccggcggc gaggccgacc
    11341 ctgacgccat cggcgcccgc tactacagca tgtaccagca gcggaaccgc atcgaagagg
    11401 tcatcggcga cctggacgcc ttccgctccc tcgtcacccg gaccggtggg cacggcactc
    11461 cggtcggagt ctgaccgcgg gtcagcgggg gagcatggtg atcccgagca tccgtgctcc
    11521 catctcggtg cggggccggt ggcggctgcc cggtgcgaag accacgtacg taccgggccc
    11581 gtggcgttcc tcgccctcga tcacctcacc gctcagcacg tagtagcgct cttcggtggc
    11641 gtgcacgtcg acgtccggcc actcggctcc cggggcgaag tcgatcagcc agccaccggc
    11701 gtatgcggtc ctcggcagtc tgcggcgcac gatcccgggg acgacctcga ccggtgcgac
    11761 ctcatcgacc tgcacggccg tcttcagcgg gcccgtttca gtggtttcac tcatggaacc
    11821 acggtgaccc gggccggcct ccagggccga gagtccagat agacatccat gggtatcttc
    11881 ctgctatgaa ccgtcatcgg gtggtggccc ttctcaaccc gccccagtcc ccctt
//
