LOCUS       NZ_CP029541            11441 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. NEAU-S7GS2 chromosome, complete genome.
ACCESSION   NZ_CP029541
VERSION     NZ_CP029541.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NEAU-S7GS2
  ORGANISM  Streptomyces sp. NEAU-S7GS2
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Streptomyces sp. NEAU-S7GS2 isolated from saline-alkaline soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) School of Life Science, Northeast
            Agricultural University, Mucai Street, Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: AMRT Link v. v5.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003173275.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 05:28:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,458
            CDSs (total)                      :: 8,365
            Genes (coding)                    :: 8,052
            CDSs (with protein)               :: 8,052
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 313
            CDSs (without protein)            :: 313
            Pseudo Genes (ambiguous residues) :: 0 of 313
            Pseudo Genes (frameshifted)       :: 144 of 313
            Pseudo Genes (incomplete)         :: 230 of 313
            Pseudo Genes (internal stop)      :: 21 of 313
            Pseudo Genes (multiple problems)  :: 74 of 313
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:52:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7528792
            Orig. end                         :: 7540233
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11441
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="28"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11441
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11441
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6441](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6441)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1395..2147
                     /gene="hemQ"
                     /locus_tag="DKG71_RS32520"
                     /old_locus_tag="DKG71_32520"
     CDS             1395..2147
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984746.1"
                     /locus_tag="DKG71_RS32520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32520"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_037741238.1"
                     /transl_table=11
                     /translation="MTDASTPASAPQTPAAPEKVPHAGKKAKDLNEVIRYTLWSVFKLR
                     DVLPEDRSGYADEVEELFAQLAAKDVVVRGTYDVSGLRADADLMIWWHSESSDALQEAY
                     NLFRRTRLGRALEPVWSNMALHRPAEFNKSHIPAFLADEQPRAYVSVYPFVRSYEWYLL
                     PDEDRRRMLADHGKMARGFPDVRANTVPSFSLGDYEWVLAFEADELYRIVDLMRHLRGS
                     EARMHVREEIPFYTGRRKPVSELVAGLA"
     gene            complement(2235..3884)
                     /locus_tag="DKG71_RS32525"
                     /old_locus_tag="DKG71_32525"
     CDS             complement(2235..3884)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (79-495). E-value:
                     3.9e-17. Score: 54.6. Matches aSDomain:
                     nrpspksdomains_DKG71_RS32525_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602445.1"
                     /locus_tag="DKG71_RS32525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32525"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_239472415.1"
                     /sec_met_domain="PF00561 (E-value: 2.7e-20, bitscore: 72.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTALATAPAGGATGAAAAPPAPVAHRIEFGRCAAVEHLPSSVEC
                     GRLAVPLDYARPDGKKIHLTVSRIRATARGERQGALVFNPGGPGASSMEFPLYGALPKW
                     SKLARAYDFVGYAPRGVGRSAPLSCQDPKEFAKAPSDSRMHPDAAFKRQKRAEAKEYAR
                     GCVRRAGADLPYYNSINNARDLDMLRAALGEKKLTFMGASYGTYFGAVYATLFPGHVRR
                     MIFDSVVNPSPRKIWYRNNLDQDVAFERRWGDWLGWVARHDKQYHLGTTKKAVQHSYDK
                     AAYKLRRKPLNGKIGAGQLQSAFLKTGYNDAFWPMRAEALSGYLHGDPKPLLAQALPRA
                     AGAAADENSNAVYTAVECNDAPWPRDWGTWDRDNTEIARIAPFETWDNAWMNLPCAYWP
                     DRSASAAAELDAAVRRADHPADGQDLVDVAGHAARTQVDDALHGRRGPLDVHTEPGALP
                     PVLLLAAERDAATPYPGALELQRRLPGSSLVTENGAGTHGVAFNDNACVNKYAEAYLLH
                     GKIPAHKVYCGPRAEPVPGQQATRAQHALPGK"
     aSDomain        complement(2400..3647)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DKG71_RS32525_Abhydrolase_1.1"
                     /evalue="3.90E-17"
                     /label="DKG71_RS32525_Abhydrolase_1.1"
                     /locus_tag="DKG71_RS32525"
                     /protein_end="495"
                     /protein_start="79"
                     /score="54.6"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASSMEFPLYGALPKWSKLARAYDFVGYAPRGVGRS
                     APLSCQDPKEFAKAPSDSRMHPDAAFKRQKRAEAKEYARGCVRRAGADLPYYNSINNAR
                     DLDMLRAALGEKKLTFMGASYGTYFGAVYATLFPGHVRRMIFDSVVNPSPRKIWYRNNL
                     DQDVAFERRWGDWLGWVARHDKQYHLGTTKKAVQHSYDKAAYKLRRKPLNGKIGAGQLQ
                     SAFLKTGYNDAFWPMRAEALSGYLHGDPKPLLAQALPRAAGAAADENSNAVYTAVECND
                     APWPRDWGTWDRDNTEIARIAPFETWDNAWMNLPCAYWPDRSASAAAELDAAVRRADHP
                     ADGQDLVDVAGHAARTQVDDALHGRRGPLDVHTEPGALPPVLLLAAERDAATPYPGALE
                     LQRRLPGSSLVTENGAG"
     misc_feature    3969..3983
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.73"
                     /tool="antismash"
     gene            complement(4008..4811)
                     /locus_tag="DKG71_RS32530"
                     /old_locus_tag="DKG71_32530"
     CDS             complement(4008..4811)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088889.1"
                     /locus_tag="DKG71_RS32530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32530"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_093648403.1"
                     /transl_table=11
                     /translation="MWLLFLLVACSAAVLSCALLCRAAVAAARATSGPAPQPPPGTVPE
                     TAAVGRRGPGLTLYEMAYLSGGPHRLADVVLVLMAQQRRLLLADTGWATVVDPVGKDAV
                     ERATLSAIGPDGQRRIPAIRDALTGDDAVREVADRLVTDGLALPTAVRPGVAGAVRLVR
                     GAALLVLPAAAASYWTAPDGADGGLLLAWFSLPLILTLGTWAVARFELHPYSDWATTAG
                     ERRLPGIGHSPATAATPASLVTLALRGPAALTDPALRAALHSSGA"
     misc_feature    4855..4869
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.68"
                     /tool="antismash"
     gene            complement(5001..6441)
                     /locus_tag="DKG71_RS44405"
                     /pseudo=""
     CDS             complement(5001..6441)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984740.1"
                     /locus_tag="DKG71_RS44405"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 3.1e-94, bitscore: 314.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIAAEIAELPGVDWVEVVAENICPGHLPSALQE
                     LRERGTTVIPHGVSLGLGGAERPDPGRLTALAERAEALGAPLVTEHIAFVRAGGPLTAS
                     PRMEAGHLLPVPRTHDALDVLCENVRIAQDALPVPLALENIAALINWPGEELTEGQFLA
                     ELVERTGVRLLIDVANLHTNHINRGEDPAVALDELPLEALAYVHVAGGVERDGVWHDSH
                     AHPVTRPVLDVLAELCARTTPPGVLLERDDDFPEGGELAGELDAIRTVLKEARHDRAA"
     gene            complement(6676..7068)
                     /locus_tag="DKG71_RS32545"
                     /old_locus_tag="DKG71_32545"
     CDS             complement(6676..7068)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088890.1"
                     /locus_tag="DKG71_RS32545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32545"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_109893499.1"
                     /transl_table=11
                     /translation="MLIGELSRRTGVKARLLRYYEAQGLLDVRREQNGYRDYDEDAVVT
                     VRRVRALLDAGLSTEVIRSVLPCVRGEAQEAPEFDWCADLRAVLQGEMTAVDEHIHNLR
                     RTRGTLAGYLAQSPGGPTRGALPRTG"
     gene            7237..8037
                     /locus_tag="DKG71_RS32550"
                     /old_locus_tag="DKG71_32550"
     CDS             7237..8037
                     /NRPS_PKS="Domain: Aminotran_4 (40-256). E-value: 1.8e-25.
                     Score: 82.0. Matches aSDomain:
                     nrpspksdomains_DKG71_RS32550_Aminotran_4.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003970024.1"
                     /locus_tag="DKG71_RS32550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32550"
                     /product="aminotransferase class IV family protein"
                     /protein_id="WP_109893501.1"
                     /transl_table=11
                     /translation="MSFVVQRNGVAATAEELAPLAFAGYAHFTALQVRDGRVRGLDLHL
                     ERLRSASLEMFGRALPDDRVRTYLRTALRAGPADLSLIATVYSPAGEFTVAGPEVEPEL
                     LVRTAPAATGPEGPLALAAVEHERELPAVKHVGEVAKTYRLRQAAEQGYDDAAFVDRRG
                     RLSEATIWNLAFWDGSSVVWPAAEMLTGTTMGIIRRQLEHLGVPQRTEEVSLDDLPALA
                     GAVVMNSWTPGIPVHRIGSVPLPDAPLFLKQLHRAYEAEPSLPV"
     aSDomain        7357..8004
                     /aSDomain="Aminotran_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DKG71_RS32550_Aminotran_4.1"
                     /evalue="1.80E-25"
                     /label="DKG71_RS32550_Aminotran_4.1"
                     /locus_tag="DKG71_RS32550"
                     /protein_end="256"
                     /protein_start="40"
                     /score="82.0"
                     /tool="antismash"
                     /translation="LDLHLERLRSASLEMFGRALPDDRVRTYLRTALRAGPADLSLIAT
                     VYSPAGEFTVAGPEVEPELLVRTAPAATGPEGPLALAAVEHERELPAVKHVGEVAKTYR
                     LRQAAEQGYDDAAFVDRRGRLSEATIWNLAFWDGSSVVWPAAEMLTGTTMGIIRRQLEH
                     LGVPQRTEEVSLDDLPALAGAVVMNSWTPGIPVHRIGSVPLPDAPLFLKQLHR"
     gene            complement(8165..8917)
                     /locus_tag="DKG71_RS32555"
                     /old_locus_tag="DKG71_32555"
     CDS             complement(8165..8917)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088892.1"
                     /locus_tag="DKG71_RS32555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32555"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_109893503.1"
                     /transl_table=11
                     /translation="MTSTETAGEPGNTATSASEHAETDRDAAPQFVLPLVVRIEREAPP
                     ARTDALETAARAVLVLLSDERSAGDGEWATAVRDWQDARIRKVVRRARRAEWRRAEALP
                     GITVTGHEAEVRVFPPVPLDGWPKDLARLQVSGTELDDPQPPGAPAAGPVLWFNPGLEM
                     SAGKEMAQAGHGAQLAWWELSEEERTAWRAAGFPLSVRSAERTEWDALTRSGLPVVRDA
                     GFTEIAPGNTVVVEGDDRFCPLPRERRP"
     gene            complement(<9009..>9104)
                     /locus_tag="DKG71_RS44410"
                     /pseudo=""
     CDS             complement(<9009..9104)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504155.1"
                     /locus_tag="DKG71_RS44410"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGRIAGDTVFGPDSPFRLKYLEPYAGAARAWL"
     misc_feature    9075..9086
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.71"
                     /tool="antismash"
     gene            9362..10270
                     /locus_tag="DKG71_RS32560"
                     /old_locus_tag="DKG71_32560"
     CDS             9362..10270
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088893.1"
                     /locus_tag="DKG71_RS32560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32560"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_109896581.1"
                     /transl_table=11
                     /translation="MRLDGVGRRYGVRGPWVLREVDLDVPPRALLRIEGTNGSGKSTLL
                     RLLAGIDRPSTGRITGRPRTAYVPERFPVALPFTAAGYLTHLGRIHGLHGPEAARRATE
                     WLERFGAAGHAHTRLAELSKGTSQKVAVAQVLLAEPGLLVLDEAWTGLDRGAREVLDRA
                     VAERVADGGTVVFVDHDPQRLAGAADARYRVADGRLLTQEESGPAAGARTAAGPRVLIE
                     AEGAPGSLPPAGAPQLPGGPEIAPGPDGTTRLTVPAVHSDALLRALLTARPPWHIRSVT
                     AAPGTAPAADEAPAPDEAPLP"
     gene            10267..10935
                     /locus_tag="DKG71_RS32565"
                     /old_locus_tag="DKG71_32565"
     CDS             10267..10935
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088894.1"
                     /locus_tag="DKG71_RS32565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32565"
                     /product="ABC transporter"
                     /protein_id="WP_109893505.1"
                     /transl_table=11
                     /translation="MIALLRYQTALLIGSHRWLPPVILYAAWLALGIRSGDPILDGFGY
                     AAGGLLPAAVWLTRVCVTNEPPAARNCAAAAAGPGRVHLACVLTAAGTALLLALVGTAV
                     VVLLSDPRSTDHQVAVPVLPATLAGLLAALACLLMGTAIGALSNWPVLRSTGWSIPAGL
                     LAALLLLVLGASPANAAVSGLVTGSLHGTISTPWLPLAATTLIAAAAVAAACALSSRRG
                     "
ORIGIN
        1 ggactggcgg ccgcgcaccg cctgctcgaa ggcggcgccc gggtgaccgt cctggaggcc
       61 tccggccggc tcggcggcaa gctgcacgcc ggcgcgatcg cgggcgtccc ggtcgatctc
      121 ggcgccgaat cgatgctggc caggcgcccg gaggcggtgg acctcgcgcg cgccgtcggg
      181 ctcggcgacc ggctgcagcc gcccgccacg gccaccgcgt cgctgtggac ccgcggtgcg
      241 ctgcgcccga tgcccaaggg gcatgtgatg ggcgtgcccg gggatctggg tctgctcgcc
      301 gcctccgggg tgatctcgcc ggacgggatg gcgcggatcg ccgaggacga gacgctggcg
      361 cggaccgagg ccggtgagga tgtcgcggtc ggcgagtatg tcgccgcccg gctcggccgg
      421 gaggtcgtcg accggctcgt cgagccgctg ctcggcggcg tctacgcggg cgacgcctac
      481 cgcatctcga tgcgcgccgc cgtcccgcag ctcttcgagg ccgcccggac acagcgttcc
      541 ctgatcgagg gcgtacgggc catccaggcg cgcgccgccg ccaccgcccc gccggagggg
      601 gccccgccgg tcttcatggg catcgacggc gggatcggca cactcccggc cgccgtcgcc
      661 gacgccgtgc gccgggccgg cggcgagatc ctcctcggga cgcctgtcga ggagctccgg
      721 tgggccgccg gggagggatg gcggatccgt ctcggtgacc ggacgatcgc ggccgacgcg
      781 gtcgtcatgg ccgcgcccgc gggcgcggcg gcgggcctgc tgcgcacgga ctgccccgcc
      841 gcggccaccg cgctggacac cgtcgactac gcctcgatgg cgctggtcac catggccttc
      901 cgccgcgccg acctggaccg ggtgcccgac ggcagcggct tcctggtacc tcccgtcgac
      961 gggcaccgca tcaaggcctc caccttcgcc agccacaaat ggggctggat cgacgccgcc
     1021 gaccccggcc tgttcgtgct gcgcacctcc ctcggccggt accaggacgc cgccgacctc
     1081 cagcgcgacg actccgaact cgtcgcactc tcgctcgccg acctcggcgc ggcggtcggc
     1141 ctgaccgccc ggccggtggc gagcacggtc acccgctggg agggcggact gccgcagtac
     1201 tccgtcggcc atctggagcg ggtcgccagg atccgcgagg cggtcgcccg gatgccgggt
     1261 gcgctgcggg tgtgcggcgc ggtctacgac ggcgtcggca tcccggcctg catcggctcg
     1321 gcgcgccgcg ccgcggacga gctcctcggc accctgacgc cggactccgt ccccgccgag
     1381 tgagaatgga caacatgaca gacgcttcca ctcccgcttc cgcgccgcag acccccgcgg
     1441 cgcccgagaa ggtgccgcac gccggcaaga aggccaagga cctcaacgag gtcatccgct
     1501 acaccctctg gtccgtgttc aagctgcgcg acgtcctccc ggaggaccgc agcggctacg
     1561 ccgacgaggt cgaggagctg ttcgcgcagc tcgcggccaa ggacgtggtg gtccgcggca
     1621 cctacgacgt gtccggtctg cgggccgacg cggacctgat gatctggtgg cactcggaga
     1681 gctcggacgc cctccaggag gcgtacaacc tcttccgccg gacccgtctc ggccgcgcgc
     1741 tggagccggt gtggtcgaac atggcgctgc accgccccgc cgagttcaac aagtcgcaca
     1801 tcccggcctt cctggccgac gagcagccgc gcgcctacgt gagcgtctac cccttcgtgc
     1861 gctcctacga gtggtacctc ctccccgacg aggaccgccg ccgcatgctc gccgaccacg
     1921 gcaagatggc ccgcggcttc ccggacgtgc gcgccaacac cgtgccgtcc ttctcgctcg
     1981 gcgactacga gtgggtgctg gcgttcgagg ccgatgagct ctaccgcatc gtcgacctga
     2041 tgcgtcatct gcgcggctcc gaggcgcgga tgcacgtccg cgaggagatc ccgttctaca
     2101 cggggcggcg caagcccgtc tccgagctgg tcgcgggtct ggcgtaaccg gacgcgcgcg
     2161 tccggccggg gaccccggac acggcgccgc gggccgcccc ctgggggacg gcccgcggca
     2221 ccaccggtcc tcggctactt ccccggcagt gcgtgctgcg cccgggtcgc ctgctggccc
     2281 ggcaccggct ccgcgcgcgg tccgcagtag accttgtgcg ccgggatctt cccgtgcagc
     2341 agatacgcct cggcgtactt gttcacacag gcgttgtcgt tgaacgcgac gccgtgcgtg
     2401 cccgcgccgt tctcggtgac cagcgaggaa ccgggcagcc ggcgctgcag ctccagcgcc
     2461 cccgggtacg gcgtcgccgc gtcccgctcg gccgccagca gcagcaccgg cggcagcgca
     2521 ccgggctccg tgtggacatc gagcggaccg cgccgcccgt ggagcgcgtc gtcgacctgg
     2581 gtccgggccg catggccggc gacatccacc aggtcctgcc cgtccgcggg gtggtccgcg
     2641 cggcgcaccg cggcgtcgag ctcggccgcg gccgacgccg accggtcggg ccagtaggcg
     2701 cacggcagat tcatccaggc gttgtcccag gtctcgaacg gggcgatccg ggcgatctcg
     2761 gtgttgtccc ggtcccaggt cccccagtcg cgtggccagg gcgcgtcatt gcactccacg
     2821 gcggtgtaga cggcgttgct gttctcgtcg gcggcggcgc cggcggcccg gggcagggcc
     2881 tgggcgagca gcggcttggg gtcgccgtgc agataccccg agagcgcctc ggcgcgcatc
     2941 ggccagaagg cgtcgttgta gccggtcttg aggaacgcgg actgcagctg gccggcgccg
     3001 atcttcccgt tgagcggctt gcggcgcagc ttgtacgcgg ccttgtcgta ggagtgctgc
     3061 accgccttct tggtcgtgcc gaggtggtac tgcttgtcgt gccgggcgac ccagcccagc
     3121 cagtcgcccc agcggcgctc gaaggcgacg tcctggtcga ggttgttgcg gtaccagatc
     3181 ttccggggcg agggattgac gacgctgtcg aagatcatcc gccggacgtg accggggaag
     3241 agcgtcgcat agaccgcacc gaagtacgtc ccgtacgagg cgcccatgaa cgtcagcttc
     3301 ttctcgccga gcgcggcccg cagcatgtcc aggtcgcggg cgttgttgat ggagttgtag
     3361 tacggcaggt ccgcgccggc ccggcggacg cagccgcggg cgtactcctt ggcctccgcc
     3421 cgcttctggc gcttgaaggc cgcatcgggg tgcatccggc tgtcggaggg cgccttggcg
     3481 aactccttgg ggtcctggca cgacagcggt gccgagcggc ccacaccacg cggcgcatag
     3541 cccacgaagt cataggcgcg agcgagcttg ctccatttgg ggagcgcccc gtagagcggg
     3601 aactccatgc tggacgcgcc ggggccgccg gggttgaaga ccagggcgcc ctgccgttcg
     3661 ccgcgcgccg tggcgcggat ccggctgacg gtgaggtgga tcttcttgcc gtcggggcgc
     3721 gcatagtcga gaggaacggc gagccggccg cactcgacgg acgacggcag atgctccacc
     3781 gccgcgcagc ggccgaattc gatgcggtgc gcaacgggcg cgggtggcgc ggcggcggcc
     3841 cccgtggcgc cgcctgccgg ggcagtggcg agggcggtca ccatcagcga cccgatggtg
     3901 ccgtacaacg caagtgctct cactggccgt cccttcgtgt gtgcatacaa aagggatagt
     3961 tggggcgctg attgtcgttg taaagcacct ggctccggtg tcggaggcta tgccccggaa
     4021 gagtggagcg ccgcgcgcaa ggcgggatcg gtgagggcgg cggggccgcg gagggcgaga
     4081 gtgacgaggg aggcgggcgt ggcagcggtg gcgggggagt ggccgatgcc gggcagtcgc
     4141 cgttcgccgg cggtggtggc ccagtcgctg tacgggtgga gctcgaagcg ggccacggcc
     4201 caggtgccga gggtgaggat cagcggcagc gagaaccagg ccaggagcag cccgccgtcc
     4261 gcgccgtccg gtgccgtcca gtaggacgcc gccgcggccg gcagcaccag gagagcggcg
     4321 ccccgcacca ggcggacggc gccggcgacg cccggacgga ccgcggtggg cagcgccagc
     4381 ccgtcggtca ccagccggtc ggccacctcc cgcaccgcgt cgtcgccggt cagggcgtcc
     4441 cgtatggccg gtatgcggcg ctgcccgtcg ggccctatgg cgctgagggt ggcgcgctcg
     4501 acggcgtcct tgccgaccgg gtcgacgacg gtggcccagc cggtgtcggc gagcagcagc
     4561 cgtcgctgct gggccatcag caccagcacc acatcggcga gccggtgcgg gccgccggag
     4621 aggtacgcca tttcgtacag ggtcagcccg gggccacggc ggcccacggc ggccgtctcc
     4681 gggacggtgc cgggcggcgg ttgcggggcc gggccggagg tggcgcgggc ggcggcgacc
     4741 gccgcacggc agagcagcgc acaggacagc accgccgccg aacaggcgac gaggaggaag
     4801 agcagccaca tgtcggtatt tttatgcgag gcgacacgat gaccgccaca aagtttctca
     4861 gtatgcgggc agttgcccgg cgtcagctgc ccggtggccg gatccgccgc cgcagccgga
     4921 ttcggcgccg cggctcgaac cgcccccgga cggcgagctg ccccccacgg ctcgcaccgc
     4981 ttggggctac gacctcgcgg tcacctcggg ccccggaaga gccgcagggc gtgtgccagc
     5041 cgggccgcgg gccgggcgga cggcggcttc ggtcccgcgc ggtcccgcca ccagcgcgcc
     5101 aactgccgcc gcgcggccgg gtcttcgggc cgcccctggg gcagcagatg cgccgcgaag
     5161 tccagggcgt cctggcggta gccgccgctc atgggccggt tccgggcatg gtccgcaaag
     5221 gccgcccagt agtccgagcc gaggatctcg ggcagctcgg gcgcgacctt ggcgaccacc
     5281 tcggcccgct tggcggccag cgcccggctc tgcacccgca gccgggtccg gtcgaagccc
     5341 tcgggggcgg gcgttccggc gacgagcgcg gacagcagcg cggtctgcgc cagcgccagc
     5401 cgctgacgcg ccgtgtcggt gccggtgtcg gtgcccgtgc cggtgcccgc atcgccaccc
     5461 atggtgccca cttcggccgg ggcgtccggg tgggtgcccg catccgggtg ggtgcccacg
     5521 tccggtgcgg tgtccggcac cggtgcggtc tccggccccg gcgtggagtc cggccccggc
     5581 gcggtgtccg gcaccgcctc aagccgcccg gtcatggcgg gcctccttca gcacggtgcg
     5641 tatcgcatcg agttcgccgg ccagttcccc gccttcgggg aagtcgtcat cccgctccag
     5701 cagcaccccg ggcggggtgg tccgggcaca cagttcggcg aggacgtcca gcacgggccg
     5761 ggtcaccggg tgggcatggc tgtcgtgcca caccccgtcc cgctccaccc cgcccgcgac
     5821 atggacgtag gccagggcct ccagcggcag ctcgtcgagg gcgaccgccg ggtcctcgcc
     5881 gcggttgatg tggttcgtgt ggagattggc gacatcgatg agcagccgta ccccggtccg
     5941 ctcgacgagc tcggccagga actgcccctc ggtcagctct tcgcccggcc agttgatgag
     6001 cgcggcgatg ttctccagcg ccagcggcac cggcagggcg tcctgggcga tccgtacgtt
     6061 ctcgcacagc acgtccagtg cgtcatgcgt gcgcggtact ggcagcagat gcccggcctc
     6121 catccggggc gaggcggtga gcggcccgcc cgcccggacg aaggcgatgt gctcggtgac
     6181 cagcggtgcg ccgagcgcct cggcgcgctc ggcgagcgcg gtcagccggc cggggtccgg
     6241 acgctcggca ccgccgaggc ccagcgagac cccgtgtggg atcacggtcg tgccgcgctc
     6301 gcgcaactcc tgcagggcgg acggcagatg gcccgggcag atgttctccg ccaccacctc
     6361 gacccagtcc accccgggca gctccgcgat ctccgcggcg atttccggcc gccagccgat
     6421 tcccgtaccc aggcgctcca tggctccccc tctcgctccg tggtgtcggt actcatgccg
     6481 cgtcagggcg gcccgaaccc tgcaccggcc gagttcagag gttgatttga gcttcaccgg
     6541 cacccccgcg gggccgcacg gtgtcgctac gggccctgct ccgccgcacc acggtgagcg
     6601 gcgcgagccg cgccccgacg gtggcctgat cggaccgggc accgggcccg gtggcgcccc
     6661 cggcctgctc acctctcagc cggtgcgcgg cagcgcccca cgggtgggac cgccgggcga
     6721 ctgtgccaag tagccggcga gggtgccgcg ggtgcgtcga aggttgtgta tgtgctcgtc
     6781 cacggccgtc atctcgccct ggaggacggc ccgcagatcg gcgcaccagt cgaactccgg
     6841 tgcttcctgc gcctcccccc ggacgcatgg caacaccgaa cggatcacct cggtggacag
     6901 gcccgcgtcc agcaacgcgc gcacgcgccg cacggtgacg accgcgtcct cgtcgtagtc
     6961 ccggtagccg ttctgctcgc gtctgacgtc caggaggccc tgcgcctcgt agtaccgcag
     7021 caaacgtgcc ttgaccccgg tacgccggga caactccccg atcagcatgg tgccccgccc
     7081 atcgcatccg agcttcgtga ttggccctcg cgccaggcct tcgcgcttga ccttcgcatc
     7141 gatgtcaagc cttaacgtcc tccaccgaac gggtattccg cacaggtgcc tgactgatgc
     7201 ctgactgatg actggctgat gctggagtga tgcacgatgt ctttcgttgt tcaacgtaac
     7261 ggtgtggcgg cgaccgcaga ggagctggcg cccctcgcct tcgcgggata cgcccacttc
     7321 accgccctgc aggtccggga cggccgggtc cggggactcg acctccactt ggagcggctg
     7381 cggtccgcct ccctggagat gttcgggcgg gcactgcccg acgaccgggt ccgtacctac
     7441 ctgcggacgg cgctgcgggc cggtccggct gacctctcgc tgatcgccac ggtctactcc
     7501 ccggcaggcg agttcaccgt ggccggacct gaggtggagc ctgagctgct ggtccgtacg
     7561 gcaccggcgg caaccggccc cgagggaccg ctggccctgg cggcggtcga gcacgaacgg
     7621 gagctcccgg ccgtgaaaca tgtcggcgag gtggccaaga cgtatcgcct ccgccaggcc
     7681 gccgaacagg gatatgacga tgcggccttc gtcgaccggc ggggccggct gagcgaggcc
     7741 accatttgga acctggcctt ctgggacggc agcagtgtgg tgtggcctgc cgccgaaatg
     7801 ctgacgggca cgacgatggg catcatccgc cgccaactgg aacacctcgg tgttccccag
     7861 cgcaccgagg aagtctccct ggacgacctg cctgcgctgg ccggagcggt cgtcatgaac
     7921 tcctggaccc ccggaatccc ggtacaccgc attggctcgg tccccctgcc ggacgccccg
     7981 ctcttcttga agcagctgca ccgggcttat gaggcggaac cttccctgcc ggtgtgacgg
     8041 tcggggcggg cgtgcgcgtt gtcctccccg tggggaacgg gcagtactgg tacctcacgc
     8101 ccatgccgct cccgtatggc atcgggacgc agtggcccgc ctaacggccg acgtacgcgc
     8161 gggactacgg ccgtcgttcg cgcgggagag gacagaagcg gtcgtctccc tcgaccacaa
     8221 cggtgttccc gggcgcgatc tctgtgaacc cggcgtcgcg caccacgggc agaccgctgc
     8281 gggtgagcgc gtcccactcc gtgcgctccg cggaccgcac ggagagtggg aagccggccg
     8341 cgcgccaggc cgtccgttcc tcctcggaga gctcccacca cgccagctgc gcgccgtgtc
     8401 cggcctgggc catctccttg cccgccgaca tctccagccc ggggttgaac cacagcaccg
     8461 ggcccgcggc gggcgccccg ggcggctgcg gatcgtccag ctccgtgccc gagacctgga
     8521 ggcgtgccag gtccttcggc cagccgtcca gcggcaccgg cgggaagacc cgcacctcgg
     8581 cctcgtggcc ggtgaccgtg atgcccggca gcgcctcggc ccgccgccac tccgcgcggc
     8641 gcgcccgccg caccaccttc cggatccggg cgtcctgcca gtcccgtacc gccgtggccc
     8701 actcgccgtc cccggccgac cgctcgtccg acagcagcac cagcaccgcc cgcgccgccg
     8761 tctccagcgc gtccgtacgc gccggcggcg cctcccgctc gatacggacg acgagcggca
     8821 gcacgaactg cggtgccgcg tcccggtcgg tctctgcgtg ctcggaagcg ctggtggcgg
     8881 tgttgccggg ctccccggcg gtctcggtgc tggtcacccg aacagtctgc cagccgccac
     8941 cgacagcgcc ccgccccgct cccgcgcccc cgccccctcc cggtttcgag tggcgggcgg
     9001 acctcaacaa gccaggcgcg ggccgccccg gcgtagggct caaggtattt gaggcggaac
     9061 ggggagtcgg ggccgaaaac ggtgtccccc gcgatgcgtc cgcgggaacg ggcgcagcag
     9121 aaaaccggtg tggctgcggc gtttcgcagg gtggtttcag gcatcaagat caggcgtcac
     9181 ccgtctggag gagtcgcagc caccgtccgg cagccgtgca tgccaacgcc gcccgcccgg
     9241 cgcgaggttg gggtgcgtac ggccgggggt gaacgcaccc ccgggcaggc accgggcggc
     9301 cgctgcgtct accgtcgggt tcggcggggg ccgccaggag gcagcggagg tgccggaacg
     9361 catgaggctc gacggggtgg gacggcggta cggggtgcgc ggcccctggg tgctgcggga
     9421 ggtggatctc gacgtgcccc cgcgggcact gctgcgcatc gaggggacca acggcagcgg
     9481 gaaatccacc ctgctgcggc tgctcgccgg gatcgaccgg cccagcaccg gacggatcac
     9541 cggccgaccg cgcaccgcct atgtgcccga acgcttcccg gtcgccctgc ccttcaccgc
     9601 cgccggctac ctcacccacc tcggccgcat ccacggtctg cacggtcccg aagcggcccg
     9661 ccgcgccacc gagtggctcg aacgcttcgg ggcggccggt catgcgcaca cccggctggc
     9721 cgagctgtcc aagggaacga gccagaaggt cgcggtcgcc caggtgctgc tcgccgagcc
     9781 cggcctcctg gtcctggacg aggcctggac cggtctggac cggggcgcgc gcgaggtgct
     9841 cgaccgtgcg gtcgccgaac gggtcgcgga cggcggcacg gtcgtgttcg tcgaccatga
     9901 cccgcagcgg ctcgcgggcg ccgccgatgc ccgctaccgg gtcgcggacg ggcggctgct
     9961 gacccaggag gagagcggcc cggcggctgg tgcgcggacc gcggccggtc cccgcgtgct
    10021 catcgaggcc gagggcgcac cggggagcct gccgcccgcc ggagcgccgc agttgcccgg
    10081 cggaccggag atcgcgcccg gaccggacgg gacgacccgg ctgacggttc cggccgtcca
    10141 ctccgacgcc ctgctgcgcg ccctgctcac cgcccggccg ccctggcaca tccgctcggt
    10201 caccgctgcc cccggcaccg ccccggccgc cgacgaagcc cccgcacccg acgaggcccc
    10261 cctcccgtga tcgctctcct ccgctatcag acggctctgc tcatcggctc gcaccgctgg
    10321 ctgccgccgg tcatcctcta cgccgcctgg ctggccctcg gcatccggag cggcgacccg
    10381 atcctcgacg ggttcggcta cgcggccggc ggactgctgc cggccgccgt atggctgacc
    10441 cgggtctgtg tcaccaacga gccgccggcc gcccgcaatt gcgccgcggc cgctgccggg
    10501 cccggacggg tgcacctcgc ctgtgtactg actgcggccg gcaccgccct gctgctcgca
    10561 ctcgtcggca cggcagtggt cgtgctgctg tcggacccgc gctccaccga ccaccaagtc
    10621 gccgttccgg tcctgcccgc gaccctcgcc gggctgctcg ccgcgctggc gtgcctgctg
    10681 atgggcaccg ccatcggcgc gctgagcaac tggccggtgc tgcgcagcac cggctggagc
    10741 atcccggcgg gtctgctcgc cgccctgctg ctgttggtgc tgggcgcctc gcccgccaac
    10801 gccgccgtat cggggctggt caccggctcc ctccacggga cgatcagcac cccctggctg
    10861 ccgctcgccg ccaccaccct gatcgcggcc gccgccgtag cggccgcgtg cgccctcagc
    10921 tcgcggcgcg ggtgaagtgg gacgcggcgc aaccggggtg cggctgagcc ggggtgtggc
    10981 tgcttcgggg cgtggcagaa ccggggtgcg gctgagccgg gtgtggctgc ttcgagacgc
    11041 gccggaaccg gggcgcggtt gaagggagga agcggagggc gcggttcaag cgagggccag
    11101 ggctctcccc cgccccctcc ccctcctccc ccttcggggg agggggcggg ggtgggttcg
    11161 ggggtggggg aggggaggag cggccgtccg cctgctcccc ccaccccagc gggcctcagt
    11221 ggtgccaggg cccggtcacc gcgaacgtcg tccccggcgt atagcaattg acatacatcg
    11281 tccggtggtc gggcgagaac gtcaccccgg cgaactcgcc ccactccggc ttctgcgggg
    11341 tgccgatgtt ctgccggccg cgcgccatcg catagacctc gccgcgccgg gtgaggccga
    11401 agacgtgctg ggcgccctcg ccgtcctcac acaccatcag g
//
