LOCUS       NZ_CP029541            10981 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. NEAU-S7GS2 chromosome, complete genome.
ACCESSION   NZ_CP029541
VERSION     NZ_CP029541.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NEAU-S7GS2
  ORGANISM  Streptomyces sp. NEAU-S7GS2
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Streptomyces sp. NEAU-S7GS2 isolated from saline-alkaline soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) School of Life Science, Northeast
            Agricultural University, Mucai Street, Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: AMRT Link v. v5.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003173275.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 05:28:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,458
            CDSs (total)                      :: 8,365
            Genes (coding)                    :: 8,052
            CDSs (with protein)               :: 8,052
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 313
            CDSs (without protein)            :: 313
            Pseudo Genes (ambiguous residues) :: 0 of 313
            Pseudo Genes (frameshifted)       :: 144 of 313
            Pseudo Genes (incomplete)         :: 230 of 313
            Pseudo Genes (internal stop)      :: 21 of 313
            Pseudo Genes (multiple problems)  :: 74 of 313
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:52:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7414355
            Orig. end                         :: 7425336
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10981
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="27"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10981
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10981
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5981](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5981)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(148..159)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            complement(641..>757)
                     /locus_tag="DKG71_RS32055"
                     /old_locus_tag="DKG71_32055"
                     /pseudo=""
     CDS             complement(641..>757)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983755.1"
                     /locus_tag="DKG71_RS32055"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32055"
                     /product="ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="GRGLLLIRSLSAASGCRPTPRGKAVWFTLPELRRPRRR"
     gene            complement(<1178..1423)
                     /locus_tag="DKG71_RS44385"
                     /pseudo=""
     CDS             complement(<1178..1423)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983755.1"
                     /locus_tag="DKG71_RS44385"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLEPPTPATWRITLPRGATAVPIARAMVRTAMQDLRATADRTTAQ
                     LLTAELVTNVLKHTRGADPIELVVELRPAGCRIEVHD"
     misc_feature    1623..1643
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            1722..2549
                     /locus_tag="DKG71_RS32060"
                     /old_locus_tag="DKG71_32060"
     CDS             1722..2549
                     /NRPS_PKS="Domain: ECH (19-267). E-value: 4e-49. Score:
                     159.1. Matches aSDomain:
                     nrpspksdomains_DKG71_RS32060_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445228.1"
                     /locus_tag="DKG71_RS32060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32060"
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="WP_109893379.1"
                     /transl_table=11
                     /translation="MSPFAGSARSTETWQHIRVTRQDGVATVTLARSDKLNALTFEAYA
                     DLRDLLAELSRERSVRALVLAGEGRGFCSGGDVEEIIGATLGMDTAQLLDFNRMTGQVV
                     RAVRECPFPVIAAVHGVAAGAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYL
                     LPRVIGLGHATRLLMLGDTVRAPEAERLGLISELAEEGRADEAAAALARRLAEGPAMAH
                     AQTKALLTAELDMPLAAAVEMDAATQALLMNSDDYAEFHAAFTEKRAPKWQGK"
     aSDomain        1779..2522
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DKG71_RS32060_ECH.1"
                     /evalue="4.00E-49"
                     /label="DKG71_RS32060_ECH.1"
                     /locus_tag="DKG71_RS32060"
                     /protein_end="267"
                     /protein_start="19"
                     /score="159.1"
                     /tool="antismash"
                     /translation="TRQDGVATVTLARSDKLNALTFEAYADLRDLLAELSRERSVRALV
                     LAGEGRGFCSGGDVEEIIGATLGMDTAQLLDFNRMTGQVVRAVRECPFPVIAAVHGVAA
                     GAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYLLPRVIGLGHATRLLMLGDT
                     VRAPEAERLGLISELAEEGRADEAAAALARRLAEGPAMAHAQTKALLTAELDMPLAAAV
                     EMDAATQALLMNSDDYAEFHAAFTEK"
     gene            2552..4879
                     /locus_tag="DKG71_RS32065"
                     /old_locus_tag="DKG71_32065"
     CDS             2552..4879
                     /GO_function="GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088826.1"
                     /locus_tag="DKG71_RS32065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32065"
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="WP_109893381.1"
                     /transl_table=11
                     /translation="MRVAVIGGGPGGLYAAALLKRLDPTREITVWERNAPDDTFGFGVV
                     LSDETLGGIEHADPEVYRALQAEFVRWDDIDIVHRGRTLTSGGHGFAALGRRRLLAVLH
                     RRCRDLGIELRFRAEAPPTAELARAYDLVIAADGVHSTTRTAHADAFGPQLTTHRCRYI
                     WLAADFAFDAFRFEIAETEHGVVQLHAYPFARPEPGHDPAAPSAGPEGSAGASTVIVEM
                     REEVWRSAGLDRCDERESAEWCAKVFTDALRGRSLRGNNSSWTAFRTVVNDRWSHGNTV
                     LLGDAAHTAHFSIGSGTKLAVEDALALAACLQEQPDTATALAAYEEERRPVVASTQRAA
                     RASLAWFEELATYLDQPPHQFAFNLLTRSRRVTHDNLRLRDAGFVTAVEDEAGTPAGTP
                     PMFTPFRLGGLTLRNRVVVSPMDMYSAADGNGTPGDFHLVHLGARAMGGAGLVMTEMVC
                     VSPEGRITPGCTGLYAPEHETAWRRVTDFVHTSAPGTAIGVQLGHSGRKGSTRLMWDGI
                     DQPLPHGNWPLVAPSALPYRPGVNQTPHALAESELALIREQFVRAATSAARAGFDLLEL
                     HCAHGYLLSGFLSPLTNHRTDAYGGDLTGRLRFPLEVFDAVRAVWPAGRPMTVRISATD
                     WADGGTTAADAVAMAEAFAEHGADAIDVSTGQVVPDERPDFGRSYQTPFADRIRNTLGV
                     PVIAVGAISSWDDVNSLVLAGRADLCALARPHLYDPHWTLHAAAEQGYVGPGAPWPLPY
                     QAGSRTPPTGRTDAPKPRLTLR"
     gene            complement(5001..5981)
                     /locus_tag="DKG71_RS32070"
                     /old_locus_tag="DKG71_32070"
     CDS             complement(5001..5981)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088827.1"
                     /locus_tag="DKG71_RS32070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32070"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_109893383.1"
                     /sec_met_domain="AfsA (E-value: 1.3e-22, bitscore: 78.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASATLTPYLRDQHHKIEGRSADGNRPSALTTTVPREYVHRTAVS
                     EVFLTNWRRGAAADSWVVSAQWPRAHTFYGPLHGLHDPILLIETVRQTGILLSHVVHRV
                     PLDHPIIWQRVRYDLAPQALRTAQRPAEVELHITDHDMVFRGKRLVSALQEFRIVCDGA
                     ELASATLDYSCHSPAVYRRLRGEYSDVALANSRALPLPGAVSPRLVAREHDRDVVLSPT
                     DRPDLWQLRVDTSHPVLFDHPVDHAPGMLMVEAARQAAQATTPGFTLPVSLECSFSRYA
                     ELDAPSWVQARTTGRGDDDRRQVEVTVEQHGRLVVSAQITSVPTP"
     misc_feature    complement(5694..5696)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6124..6720)
                     /locus_tag="DKG71_RS32075"
                     /old_locus_tag="DKG71_32075"
     CDS             complement(6124..6720)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602525.1"
                     /locus_tag="DKG71_RS32075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32075"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_229838404.1"
                     /transl_table=11
                     /translation="MRTRRAVLEAAAHVIGTRGYQAATMAEIIQRAGVTKGAVYFHFTS
                     KDALARAVITEQTDPFLPQVSESRLQDAIDFTHQVALALRSDPLLQAGTRIAVETTFSE
                     DPLVPYQAWTDIITTMFTEARDNGELLPGVAPDRAAEFFVAAYMGVQLFSRAATNRADL
                     PERVTALWKHTLPGLASPGALSHLDPHGRCVKVPV"
     misc_feature    complement(6331..6333)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6837..6855)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.84"
                     /tool="antismash"
     gene            6949..7974
                     /locus_tag="DKG71_RS32080"
                     /old_locus_tag="DKG71_32080"
     CDS             6949..7974
                     /NRPS_PKS="Domain: PKS_ER (35-339). E-value: 1.9e-20.
                     Score: 65.2. Matches aSDomain:
                     nrpspksdomains_DKG71_RS32080_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088829.1"
                     /locus_tag="DKG71_RS32080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32080"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_109893385.1"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.4e-17, bitscore:
                     62.8, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPTVVPGIPALHREVRLAARAEGELTAHHFMIAEVPVPEPEPGQ
                     VLVRNRVMAVTAAMRTQMTGIRLPMASFVPGQALWGSAVGEVVAAPGGGFTPGELVHHP
                     YGWREFAAVEESRVRSLTEDPLPTPAAHLSQGATAWGALRLAAEVRPGDTVFISGAAGG
                     VGSMAGQLARRLGAGRVIGSTGSERKAARLRAELGYDDTVVRGAGPIERQLRRAAPDGI
                     DVLLDTVGGEQLTAALAVARADARFALVGALSSQLGGSGAIAPVELDPGLIIMRRVALR
                     GFGLHAHPDLPQEWTKEFGQGLREGSLVFPHALLKGIQQAPRALSELTQGRHIGAVLVE
                     L"
     aSDomain        7054..7965
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DKG71_RS32080_PKS_ER.1"
                     /evalue="1.90E-20"
                     /label="DKG71_RS32080_PKS_ER.1"
                     /locus_tag="DKG71_RS32080"
                     /protein_end="339"
                     /protein_start="35"
                     /score="65.2"
                     /tool="antismash"
                     /translation="VPVPEPEPGQVLVRNRVMAVTAAMRTQMTGIRLPMASFVPGQALW
                     GSAVGEVVAAPGGGFTPGELVHHPYGWREFAAVEESRVRSLTEDPLPTPAAHLSQGATA
                     WGALRLAAEVRPGDTVFISGAAGGVGSMAGQLARRLGAGRVIGSTGSERKAARLRAELG
                     YDDTVVRGAGPIERQLRRAAPDGIDVLLDTVGGEQLTAALAVARADARFALVGALSSQL
                     GGSGAIAPVELDPGLIIMRRVALRGFGLHAHPDLPQEWTKEFGQGLREGSLVFPHALLK
                     GIQQAPRALSELTQGRHIGAVLV"
     CDS_motif       7402..7464
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DKG71_RS32080_0001"
                     /evalue="1.70E-04"
                     /label="PKSI-ER_m5"
                     /locus_tag="DKG71_RS32080"
                     /protein_end="172"
                     /protein_start="151"
                     /score="14.3"
                     /tool="antismash"
                     /translation="GDTVFISGAAGGVGSMAGQLA"
     gene            complement(8015..8647)
                     /locus_tag="DKG71_RS32085"
                     /old_locus_tag="DKG71_32085"
     CDS             complement(8015..8647)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088830.1"
                     /locus_tag="DKG71_RS32085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32085"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_109893387.1"
                     /transl_table=11
                     /translation="MPTQHRAIQSRQALIRSAAETFLEKGVPAAGMVEISRRARLSKGA
                     LYFHFTSKDDLTLAVRDAALEALQELEDAFTSSPQPLTTAVRDFTVELFGRVESDAVLR
                     AGLRLRPETAPGLGIYALEQRWYALFLDKAVTCGTGGPAGPHASDTEPRRTAQLLTSIV
                     VGLLHLGSEDGTWWDEEAIVGLWALLPSAPGRIPAAVPTPAQLPAAG"
     misc_feature    8895..8909
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     misc_feature    8921..8935
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.22"
                     /tool="antismash"
     gene            9129..9860
                     /locus_tag="DKG71_RS32090"
                     /old_locus_tag="DKG71_32090"
     CDS             9129..9860
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602521.1"
                     /locus_tag="DKG71_RS32090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32090"
                     /product="HAD family hydrolase"
                     /protein_id="WP_229838405.1"
                     /sec_met_domain="HAD_2 (E-value: 1.2e-27, bitscore: 96.1,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRGVRGAGGETIRAAVFDLDGTLANTLPAISKLLIKVASEQGCSV
                     TARQAAAAIGKPPGPAFGRLLGKPEDDGRVQAAISRYRELFAYEVLCLGPQLLFPGVTA
                     GLSALRAHGVELAVATSKTTRSAEALLDVMGIRDLVGPVIGQDMVRRGKPHPEMVLRAA
                     GRLGVAAWQSAYVGDTVGDMRMAVTARMEPVAVTYGVGTFGELAAVAGTRMCSSFKDVV
                     SVIAAARPRSGVTVGAQQRRR"
     gene            complement(9880..10731)
                     /locus_tag="DKG71_RS32095"
                     /old_locus_tag="DKG71_32095"
     CDS             complement(9880..10731)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602520.1"
                     /locus_tag="DKG71_RS32095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_32095"
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="WP_018088832.1"
                     /transl_table=11
                     /translation="MNDQQTQRTPRSLIVTFYGAYGRGGPDTPGGTAAPVPVAALIRLL
                     GALGVDPPSVRSAVSRLKRRGLLVPARTASGAAAYGLSEAARQLLEDGDRRIFGRPAAR
                     LSDGWVLAVFSVPEEERHKRHLLRSRLARLGFGTAAPGVWIAPAQLYEETRHTLERLQL
                     DPYVDLFTGTHAGFAPTADAVARWWDLDAVAAQHRAFLAEQEPVLQRWSRRRSVPPEAA
                     YRDYLLALDAWRHLPYADPGLPSPLLPEDWPGGRAAEVFGRLHTTLRAAGARYAHEAMT
                     GA"
ORIGIN
        1 gtctccgcca tcggcgcgct ctccgccgac caggtcgccg gccggcccgc cgcgctgtcg
       61 ctgatcgtca accacgtcac ccactcgtcg ctgggcggcg gggtgatcgc attcggcgcc
      121 gtcgtggcca tcgcctcggt cgtccttgcg gtcatgtacg ggcagacccg catcctgatg
      181 tcgatgtcgc gggacggact ggtcccgcgg atcttcgagc gggtctcgcc ccggacgtcc
      241 acccccgtcg ccaacacctg gatcgtcgcg gtcgtcttcg ccgtgccggc cgcggtcgtc
      301 ccgctggacg cggtgatgaa cctgtcgacc atcgggacgc tggcggtcat ggcggtggtc
      361 aacctcagtg tgatggtgct ccgccgcacc cgccccgatc tgccgcgccg cttccgtgtc
      421 ccgctcttcc cggtcagccc ggtgctcggc gtcggcttct gcctctacct gatgtacggc
      481 accggtccgg cgacctggct ccagttcgcc gggttcctgg tggccggtgc gctgctctac
      541 acgtgctacg ggcgccgtcg ctcgcggctc ggcgcggcgt cgcaggaccc gacgttgcag
      601 gacccggcgg ccggggccga cgaggtcggt gcggccaccg tcagcgccgg cgcgggcgcc
      661 gcagctcggg aagggtgaac cagacggcct tgccgcgcgg tgtcggacgg cagccggagg
      721 cggcgctgag ggaccggatc agcagcagac cacggccgtc gtggccccct ccgcagcctg
      781 gagcggcgga aggggcgtcg acgccgatcg cggcacaggc gtcgtccgcg ccgtgggagt
      841 cgtcgcggat ctccgcatct ttggcggtgt cggcaggggc gtcggtgtcg gcgtcggtgt
      901 agtcgtcggg atcggcacgc tcgtacccgt cgtcctggcc gtccgtgtcg tgctcgtcgt
      961 ccgcgtcgtg gtcctcgggc ccgtcgggtt cctcgccgtg cccgccctgc tgcccggtgt
     1021 cctccgcgcg ctgcgtcggg acatggccgt cggccgctga ggggccgtcc gctgtgcgga
     1081 ccggcggggg accggccggt gccaacgcca ccaccggggc gcccggttcc accaggggaa
     1141 gcggctcgct gagtccctcg acgagccgcg gactgtcgtc gtgcacctcg atccggcagc
     1201 ccgccggccg cagctccacc accagctcga tggggtcggc gccgcgggtg tgcttgagca
     1261 cgttggtgac cagctccgcg gtcagcagct gtgcggtggt ccggtcggcc gtcgcccgga
     1321 ggtcctgcat ggcggtccgg accatcgcac gggcgatggg taccgcggtc gcaccgcgcg
     1381 gcagcgtgat gcgccaggtc gcaggggtgg gcggttccag catgcgcggt cgtccgttca
     1441 caaagggccg ggtcggaggc ctgaggccgg atcggatacc cccaccgtag agagcgcgga
     1501 ggcggggaat caggggcgag cggccggggc cggcatgacc tacgtcctgc ggcggccggg
     1561 accttcggca gggtcacccc gacggccccg acggccccga cggccccgac ggcagaagtc
     1621 gtatcgcgtt ctcatgacga cagtcatgaa cgagtgataa cttcgaagag catccggaac
     1681 agcatccacc ggccgaccgg ccaaaggggg caggcagtcc gatgagcccg ttcgcaggat
     1741 ccgcccgcag tacagagacc tggcagcaca tccgagtgac gaggcaggac ggggtcgcca
     1801 ccgtcaccct ggcccgctcg gacaagctca acgcgctcac cttcgaggcc tacgccgacc
     1861 tccgcgatct gctcgcggag ctgtcccggg agcgttcggt acgcgccctg gtgctcgcgg
     1921 gggaggggcg cggattctgc tcgggcggcg atgtcgagga gatcatcggc gccaccctgg
     1981 gcatggacac cgcgcagctg ctggacttca accggatgac cgggcaggtc gtccgggcgg
     2041 tgcgggagtg tccgttcccg gtgatcgcgg cggtgcacgg ggtcgcggcg ggggcgggcg
     2101 cggtgctcgc gctggccgcg gacttccggg tcgccgaccc gtccgcccgg ttcgccttcc
     2161 tcttcacccg ggtcggactg tccggcggcg acatgggcgc ggcctatctg ctgccccgcg
     2221 tcatcggtct cggccatgcc acccggctgc tgatgctcgg cgacaccgtc cgtgcgccgg
     2281 aggccgaacg tctcggtctg atcagcgagt tggccgagga gggccgggcc gacgaggcgg
     2341 ccgcggcgct cgcccggcgg ctcgccgagg ggcccgcgat ggcgcacgcc cagaccaagg
     2401 cgctgctcac cgccgagctc gacatgccgc tggccgccgc cgtggagatg gacgccgcca
     2461 cccaggcgct gctgatgaac agcgacgact acgcggagtt ccatgccgcc ttcaccgaga
     2521 agcgggcccc caagtggcag ggcaaatagc catgcgggtc gccgtcatcg gcggcgggcc
     2581 gggcgggctg tacgccgccg ccctgctcaa gcgcctcgac cccacccgcg agatcaccgt
     2641 atgggagcgc aacgcacccg atgacacctt cggcttcggc gtcgtgctct ccgacgagac
     2701 ccttggcggc atcgagcacg ccgacccgga ggtctaccgc gccctccagg cggagttcgt
     2761 ccgctgggac gacatcgaca tcgtccaccg cggccgcacc ctgacctccg gcgggcacgg
     2821 cttcgcggcg ctcggacggc gccggctgct ggccgtgctc caccggcgct gccgcgacct
     2881 cggcatcgag ctgcgctttc gcgccgaggc cccgccgacc gccgaactgg cgcgcgcgta
     2941 cgacctggtg atcgccgccg acggggtgca cagcacgacc cgcaccgcac acgccgacgc
     3001 cttcggtccg cagctgacca cccaccgctg ccgctacatc tggctcgccg ccgacttcgc
     3061 cttcgacgcc ttccgcttcg agatcgcgga gaccgagcac ggcgtcgtac agctccacgc
     3121 ctaccccttc gcccgtccgg agcccggtca cgatcccgcg gcaccgtccg ccgggcccga
     3181 gggctccgcc ggcgccagca cggtcatcgt cgagatgcgc gaggaggtct ggcggtcggc
     3241 cggactcgac cgctgcgacg agcgcgagtc ggccgaatgg tgcgccaagg tcttcaccga
     3301 cgccctgcgc ggccggtcgc tgcgcggcaa caactccagc tggaccgcgt tccgcacggt
     3361 cgtcaacgac cgctggtcgc acggcaatac ggtgctgctc ggcgatgccg cgcacaccgc
     3421 gcacttctcc atcggctccg gcaccaagct cgccgtcgag gacgccctgg cgctggcggc
     3481 ctgtcttcag gagcagcccg acaccgccac cgccctggcc gcctacgagg aggagcgccg
     3541 ccccgtcgtc gcgtccaccc agcgtgccgc ccgcgccagc ctcgcctggt tcgaggagct
     3601 ggccacctac ctcgaccagc cgccgcacca gttcgccttc aacctcctca cccgcagccg
     3661 ccgggtcacc catgacaatc tgcggctgcg cgacgccgga ttcgtcacgg ccgtcgagga
     3721 cgaggcgggc accccggccg gcacaccgcc catgttcacg ccgttccggc tgggcgggct
     3781 gaccctgcgc aaccgggtcg tggtctcccc gatggacatg tactccgccg cggacggcaa
     3841 cggcaccccc ggcgacttcc acctggtcca cctcggcgcc cgcgcgatgg gcggcgccgg
     3901 actggtgatg acggagatgg tgtgtgtcag ccccgagggg cggatcacgc ccggctgtac
     3961 ggggctgtac gcgccggagc acgagacggc ctggcgccgg gtcaccgact tcgtgcacac
     4021 ctccgccccc ggcaccgcca tcggcgtcca gctcggccac tccggccgca agggctcgac
     4081 ccggctgatg tgggacggta tcgaccagcc gctcccgcac ggcaactggc ccctcgtggc
     4141 accctccgcg ctgccctacc gccccggcgt caaccagacc ccgcacgccc tcgccgagtc
     4201 cgaactcgct ctcatccgcg agcagttcgt acgcgcggcc acgtccgccg cccgcgccgg
     4261 cttcgatctt ctcgaactcc actgcgccca cggctatctg ctctccggat tcctctcacc
     4321 gctcaccaac caccgcaccg acgcctacgg cggcgatctc accggccggc tgcgcttccc
     4381 gctggaggtc ttcgacgcgg tccgcgccgt ctggccggcc gggcgcccga tgacggtccg
     4441 tatctccgcg accgactggg cggacggcgg caccaccgcg gccgacgcgg tggcgatggc
     4501 ggaggccttc gccgagcacg gtgccgatgc catcgatgtg tccaccggcc aggtggtgcc
     4561 cgacgagcgt cccgacttcg gccgctccta ccagaccccg ttcgccgacc gtatccgcaa
     4621 caccctcgga gtgccggtca tcgcggtcgg cgccatctcc tcctgggacg acgtcaactc
     4681 cctggtgctg gccgggcggg cggacctgtg cgcgctggcc cgaccgcatc tctacgaccc
     4741 gcactggacg ctgcacgccg ccgctgagca ggggtacgtg gggccgggcg ccccctggcc
     4801 gctgccgtac caggcgggca gccgcactcc gcccaccggg cgcacggatg cgccgaagcc
     4861 ccgcctcacg ctgcggtgac ggggaggcac gggcagcggg gcggggccgt tacggggccg
     4921 gtccgaaagg gggcgtcggc gggccggtga ggtccggcgg ggaaaccgtc aggggaccgg
     4981 cgggccggtg cggcggccgg tcagggcgtc ggcacgctgg tgatctgggc cgacacgacc
     5041 agccggccgt gctgttcaac ggtgacctcg acctgccggc ggtcgtcgtc gccccgtccg
     5101 gtggtccgcg cctgaaccca ggacggcgca tcgagttcgg cgtaccgcga gaaggagcac
     5161 tccaaggaga ccggcagcgt gaagccgggc gtcgtcgcct gtgcggcctg ccgggcggcc
     5221 tcgaccatca gcatgccggg ggcgtggtcc acggggtggt cgaagaggac cgggtgcgag
     5281 gtgtccaccc gcagctgcca gaggtcgggc cggtcggtgg gggagagcac cacatcgcgg
     5341 tcgtgttccc gggcgaccag ccgcgggctc acggccccgg gcagcggcag cgcccgggag
     5401 ttggcgagcg ccacatcgct gtactcgccg cgcagccgac ggtagacggc cgggctgtga
     5461 caggagtagt cgagcgtcgc ggaggccagc tcggcgccgt cacacacgat ccggaactcc
     5521 tggagggcac tgacgagccg cttgccgcgg aagaccatgt cgtggtcggt gatgtgcagt
     5581 tcgacctcgg cgggccgctg cgcggtgcgc agcgcctgcg gcgcgaggtc gtagcggacc
     5641 cgctgccaga tgatcgggtg gtcgagcgga acccggtgca cgacatggct cagtaatatg
     5701 ccggtctgcc gcaccgtctc tatcagcagg atcgggtcgt gcagcccgtg gagcggaccg
     5761 tagaaggtgt gggcgcgcgg ccactgggcg ctgaccaccc agctgtcggc cgccgcgccg
     5821 cgccgccagt tcgtcaggaa cacctcggaa acggccgtgc ggtggacgta ctcgcgcggc
     5881 acggtggtgg tgagcgccga cggtcggttt ccgtcggcgg accggccttc gatcttgtgg
     5941 tgctgatcgc gcaagtacgg cgtgagcgtg gcgctggcca taggtggttc ctttctgagg
     6001 tgcggctctc agcgccctga cgggctgagg cttggtaacg gcccgccact aagatacgga
     6061 ccatgtggtt tgttttccag agccgaaaga gccggcggct ctgtcagggc ggcggctccg
     6121 gtgtcagacc gggaccttca cgcagcggcc gtgcggatcc agatggctca gcgccccggg
     6181 tgacgcgagc cccggaagcg tgtgtttcca cagggccgtg acccgctccg ggagatcggc
     6241 gcggttggtc gcggcgcggg agaacaactg cacgcccatg taggccgcga cgaagaactc
     6301 ggccgcccgg tccggtgcca caccgggcag taattccccg ttgtcccggg cctcggtgaa
     6361 catcgtggtg atgatgtcgg tccaggcctg gtacggcacc agcggatcct cactgaaggt
     6421 cgtctccacc gcgatccggg tgccggcctg cagcaacggg tcgctgcgca gggccagcgc
     6481 cacctggtgg gtgaagtcga tggcgtcctg cagccgggac tcgctgacct gcggcagaaa
     6541 cggatcggtc tgctccgtta tgaccgcgcg ggccagtgcg tccttggacg tgaaatggaa
     6601 gtacaccgct cctttggtga ccccggcgcg ctggatgatc tcggccatcg tggcggcctg
     6661 gtacccgcgg gtgccgatga catgagccgc tgcttcgagg acggcgcgtc gggtgcgcac
     6721 tgcgcgttcc tgcttgacca ccgatcatcc tccgtatgct ctcttggagt tgttgtgcct
     6781 gagtgcgacc cgctgcccgt ggccgcgcag tctatttccg catcataaag aagccaacca
     6841 tgcggtattg aatttgcgcc cggtccggag gcgggccccg gcgggtagga accggcgcag
     6901 ccctccgggg cggcgccatc gagtgctacg agcgcgagga gctgtccgat gtcccccacc
     6961 gtcgtccccg gcatacctgc cctgcaccgc gaggtccggc ttgccgcccg ggccgaaggc
     7021 gagctgacgg cacaccattt catgatcgcc gaggtgccgg tgccggagcc ggaaccaggg
     7081 caggtgctgg tacgcaaccg cgtcatggcg gtgaccgcgg cgatgcgcac gcagatgacc
     7141 ggaatccgtc tgcccatggc ctcattcgtg ccgggtcagg cgctgtgggg ctcggcggtc
     7201 ggcgaggtgg tggcggcgcc cggaggcggc ttcacgcccg gtgaactggt ccatcacccc
     7261 tacggatggc gggagttcgc cgcggtcgag gagtcgcggg tacggagcct caccgaggat
     7321 ccgctgccga ccccggccgc gcatctgtcg cagggggcga ccgcctgggg tgccttgcgc
     7381 ctggccgccg aggtcagacc gggggacacc gtgttcatca gcggcgcggc gggtggcgtg
     7441 ggcagcatgg ccggacagct cgcacggcgg ctgggcgccg ggcgggtcat cggcagcacc
     7501 ggctcggagc gcaaagccgc gcggctgcgc gccgagttgg gctacgacga cacggtggtg
     7561 cgcggcgccg ggccgatcga gcggcagctg cggcgggcgg cgccggacgg tatcgatgtg
     7621 ctgctggaca ccgtgggcgg cgagcagctc accgcggcgc tcgcggtggc ccgggccgat
     7681 gcgcgattcg cactggtcgg cgcgctctcc agccagttgg gcggcagcgg cgccatcgcc
     7741 ccggtggagc tcgacccggg gctgatcatc atgcggcggg tggcactgcg cggcttcggg
     7801 ctgcacgcgc atccggatct gccgcaggag tggaccaagg agttcggcca gggcctgcgg
     7861 gagggctcgc ttgtcttccc gcacgccctt ctgaagggga tccagcaggc accgcgggcg
     7921 ctgagtgaac tgacacaggg gcgtcatatc ggggccgtgc tggtcgagtt gtgaggccgg
     7981 ggcacgaggg ccggggcgga ccgggagcgc cgggtcagcc ggcggccggg agctgagccg
     8041 gagtcggcac cgcggccggg atccggcccg gagcggacgg cagcagcgcc cacaggccga
     8101 cgatggcttc ttcgtcccac cacgtcccgt cctcgctgcc cagatgcagc agacccacca
     8161 cgatcgaggt caacagctgg gccgtgcgcc gcggttcggt gtccgaggca tgcggtccgg
     8221 ccggcccgcc ggtcccgcac gtcaccgctt tgtcgagaaa cagcgcatac cagcgctgct
     8281 ccagggcgta gataccgagc cccggcgcgg tctccggccg cagccgcagt ccggcgcgca
     8341 gtacggcgtc cgactccacc cggccgaaca gctcgacggt gaagtcccgt acggcggtgg
     8401 tcagcggctg cggggagctg gtgaaggcgt cctcgagctc ctggagggcc tccagcgcgg
     8461 cgtcccgcac cgcgagcgtg aggtcgtcct tggaggtgaa gtggaagtag agcgcccctt
     8521 tgctcagccg ggcccgccgg ctgatctcga ccatgcccgc tgcgggaacc cccttctcca
     8581 ggaaggtctc tgcggccgag cggatgagtg cctggcggct ctggatggcg cgatgctggg
     8641 tgggcacggt gtccgtcccg tctctagtac ggctgcgagc tcattgcagt ctacatagaa
     8701 aaccaaccat gcggttcgat atgcttcagg ggattggccg gaactggtgg gccctgacgt
     8761 gaaggcggcg atcaaccgct cgttccggcg ctcggcgcct cgcggggggc atgtgccccc
     8821 agtctggctc cgtcaggggg tgttgtcgac ccgtcagggc tgacgaggat gcattgacaa
     8881 acagcgcagg cggtttttaa atctccgaag gcggtaaacg ggacgttccc ttccgtggca
     8941 ccgcctcgac ggttgcgtac cgccttggac ggcggtgcgc catggcgaga ccgccgctgc
     9001 cgttaccagt cgtcgaccgc aggccgcccc atcagccgag ccagcaacgc caccagagcc
     9061 cctgcacccc ccatcgcaat ccggaaaggt ctccagccat gcgcgtagca acggaagtcg
     9121 aacgtcccgt gagaggtgta cggggcgccg gaggtgagac gatcagggcc gccgtcttcg
     9181 acctggacgg cactctcgcc aacaccctcc ccgcgatcag caaactgctg atcaaggtgg
     9241 cctcggagca gggatgttcg gtgaccgccc ggcaggcggc cgccgccatc ggcaagcctc
     9301 ccggccccgc cttcggccgg ctgctgggca agcccgagga cgacggccgg gtgcaggccg
     9361 ccatctcccg ctaccgcgaa ctgttcgcct acgaagtgct gtgcctgggg ccgcagctgc
     9421 tcttccccgg tgtgaccgcg gggctgtccg cgctgcgcgc ccacggcgtc gagctggccg
     9481 tcgccacgtc caagaccacc cgcagcgcgg aggcgctgct cgacgtcatg ggtatccgtg
     9541 acctggtcgg cccggtcatc ggccaggaca tggtccggcg cggcaaaccg cacccggaga
     9601 tggtgctgcg ggcggccggc cggctcggtg tcgcggcctg gcagagcgcg tatgtggggg
     9661 acaccgtggg ggacatgagg atggcggtca ccgcgcgcat ggagccggtc gcggtgacct
     9721 acggcgtcgg cacgttcggc gagctcgccg cggtcgccgg cacccggatg tgcagctcct
     9781 tcaaggacgt ggtctccgtg atcgccgccg cccggccgcg ctcgggcgtc acggtggggg
     9841 cccagcagcg ccgtcgctga ggccggtggg cgggggccgc tacgccccgg tcatcgcctc
     9901 gtgcgcgtaa cgggccccgg cggcccggag cgtggtgtgc agtcggccga acacctcggc
     9961 cgcgcggccg cccggccagt cctcgggcag gaggggagag ggcagtccgg gatccgcgta
    10021 gggcagatgg cgccaggcgt ccagcgccag cagatagtcg cggtaggccg cttcgggggg
    10081 caccgagcgg cgccgcgacc accgctggag gacgggctcc tgctcggcga ggaaggcgcg
    10141 gtgctgtgcg gcgacggcgt ccaggtccca ccagcgcgcg accgcgtcgg ccgtgggggc
    10201 gaagccggcg tgcgtaccgg tgaacagatc gacgtacggg tccagttgca gccgctccag
    10261 ggtgtgccgg gtctcctcgt agagctgcgc gggggcgatc cacacacccg gcgccgcggt
    10321 gccgaagccg agccgggcca gccgggagcg cagcagatgc cgcttgtgcc gctcctcctc
    10381 ggggacggag aagacggcca gcacccagcc gtcggacagc cgcgcggcgg gccggccgaa
    10441 gatccgccgg tcgccgtcct ccagcagctg gcgggccgcc tcggacagtc cgtacgcggc
    10501 tgcgccggag gccgtccgcg cggggaccag cagcccgcgc cgcttgagcc gggagaccgc
    10561 ggagcgcacc gacggcggat cgaccccgag cgcaccgagc agccggatca gggcggccac
    10621 cggcaccggg gcggccgttc cgcccggggt gtcggggccg ccgcggccgt aggcgccgta
    10681 gaacgtgacg atcagggacc gtggggtgcg ctgcgtttgc tggtcgttca ccgggtcact
    10741 ctagatgggg tccccgcagc cggaagcgct ggagcttgcc ggtcggggtg cgcggcagcg
    10801 aggtgtggaa gacgatctcg cgcgggcatt tgtagggcgc gacgcgggtc ttggtgaagg
    10861 cgcgcagcgc ggcgacggtg tcgtcgcccc gggggacgcc ggcccgcagc acgacatggg
    10921 cgacgaccac ctgcccgcgg tcctcgtcgg gccgtccgac caccgccgcc tcggtcacgt
    10981 c
//
