LOCUS       NZ_CP029541            10417 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. NEAU-S7GS2 chromosome, complete genome.
ACCESSION   NZ_CP029541
VERSION     NZ_CP029541.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NEAU-S7GS2
  ORGANISM  Streptomyces sp. NEAU-S7GS2
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Streptomyces sp. NEAU-S7GS2 isolated from saline-alkaline soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) School of Life Science, Northeast
            Agricultural University, Mucai Street, Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: AMRT Link v. v5.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003173275.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 05:28:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,458
            CDSs (total)                      :: 8,365
            Genes (coding)                    :: 8,052
            CDSs (with protein)               :: 8,052
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 313
            CDSs (without protein)            :: 313
            Pseudo Genes (ambiguous residues) :: 0 of 313
            Pseudo Genes (frameshifted)       :: 144 of 313
            Pseudo Genes (incomplete)         :: 230 of 313
            Pseudo Genes (internal stop)      :: 21 of 313
            Pseudo Genes (multiple problems)  :: 74 of 313
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:52:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2696406
            Orig. end                         :: 2706823
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="17"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5417
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(2..991)
                     /locus_tag="DKG71_RS11100"
                     /old_locus_tag="DKG71_11100"
     CDS             complement(2..991)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536882.1"
                     /locus_tag="DKG71_RS11100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_11100"
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_162597055.1"
                     /transl_table=11
                     /translation="MPRRLAATLVATALTAGPAVLLGAVPAHATGDHGPARGTADAVVL
                     RTGVNVGLLNKTLDVPLNVALNEVHAPASASKTALTVKLDGIDHGRPFSVLRADAATAR
                     ATADRRKAEGYANVVRAKVHLPGLPLLSLIEVQQVTAKAVCEAGTRPRAESNVLGSVRV
                     LGKKVTLTAGGTTDVKVPGVGRVRLDLSKKNVTSKGAAATALDLNVSLNPLKLNVADVQ
                     GRVTLAKATCTTPRAKAPEQGGSGGTSGGQTGGGSTGGQTGGGTEPAGGKDTEVTGSGP
                     RTQNLAETGGSSATPYLAGGAALLLAAGAGSMVFARKRRSAAPQGGRG"
     misc_feature    1157..1170
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.47"
                     /tool="antismash"
     gene            complement(1279..2370)
                     /locus_tag="DKG71_RS11105"
                     /old_locus_tag="DKG71_11105"
     CDS             complement(1279..2370)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960017.1"
                     /locus_tag="DKG71_RS11105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_11105"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_109888948.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.6e-16, bitscore:
                     59.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEGAVLHLKGRVLVGPDEVRDELWVVGGRVTFDRPVMARDAITV
                     TGWALPGLVDAHCHVGLDAHGPVDAPTSEKQALTDRDAGTLLIRDAGSPSDTRWVDDRE
                     DLPRIIRAGRHIARTKRYIRNYAHEIEPEDLVAYVAREARRGDGWVKLVGDWIDRSTGD
                     LEACWPRGAVEAAIAEAHRLGARVTAHCFAEDTLAPLVEAGIDCIEHATGLTEETIPLF
                     AERGVAIVPTLVNIATFPRLADGGEAKFPRWADHMRRLHDRRYDTVRAAYDAGVPVFAG
                     TDAGGSLAHGLVAEEVVELTRAGLPAVAALSATTWGARDWLGRPGLTEGAPADLVVYES
                     DPREDVRVLAAPRRVVLRGQIVG"
     misc_feature    2608..2621
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(2799..2814)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(2809..2824)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     gene            2980..3549
                     /gene="ectA"
                     /locus_tag="DKG71_RS11110"
                     /old_locus_tag="DKG71_11110"
     CDS             2980..3549
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536877.1"
                     /locus_tag="DKG71_RS11110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_11110"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_026170006.1"
                     /transl_table=11
                     /translation="MTAAQADHAGARSDIREFPEGFKLDTPRVEDGAAIWRIARDSKTL
                     DLNSSYSYLLWCRDFAATSVVARDTEGEPAAFITGYLRPERPETLVVWQVAVDDAHRGQ
                     GLAAALLDGLTARARDELGVRFVETTVTPDNTASNRLFASFAERHSVPLKSEVLFDAGL
                     FPEQGHEPEVLHLIGPFEPPAGTGHS"
     gene            3686..4954
                     /gene="ectB"
                     /locus_tag="DKG71_RS11115"
                     /old_locus_tag="DKG71_11115"
     CDS             3686..4954
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 6e-76.
                     Score: 247.6. Matches aSDomain:
                     nrpspksdomains_DKG71_RS11115_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="DKG71_RS11115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_11115"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_093642852.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.5e-79, bitscore:
                     264.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRSWPTVFDRAQGSRMTDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGANSVEAALKLARKVKGRESIVSFTNAFHGMSLGALAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYLDGTYPDFLWFERLLEDQGSGLNTPAAVIVETVQGEGGINVARP
                     EWLRALADLCERWDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAATLDTYWADGQMEKQTLARGEIVEAHLK
                     AIAEEHPDAIAEYRGRGLVWGMELNDKPTADKIAKRAFELGLLIETSGPESEVVKLLPA
                     LTTTPEELDEGLRVLARAVRDCV"
     aSDomain        3770..4780
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DKG71_RS11115_Aminotran_3.1"
                     /evalue="6.00E-76"
                     /label="DKG71_RS11115_Aminotran_3.1"
                     /locus_tag="DKG71_RS11115"
                     /protein_end="365"
                     /protein_start="28"
                     /score="247.6"
                     /tool="antismash"
                     /translation="FDRAQGSRMTDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGANSVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGALAVTGNSMKRAGAGIPLVHGTPMPFDNYLDGTYPDFLWFER
                     LLEDQGSGLNTPAAVIVETVQGEGGINVARPEWLRALADLCERWDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAATLDTYWADGQMEKQTLARGEIVEAHLKAIAEEHPDAIAEYRGRGLVWGMELN"
     gene            5001..5417
                     /locus_tag="DKG71_RS11120"
                     /old_locus_tag="DKG71_11120"
     CDS             5001..5417
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606340.1"
                     /locus_tag="DKG71_RS11120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_11120"
                     /product="ectoine synthase"
                     /protein_id="WP_018091828.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.1e-53, bitscore:
                     178.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETTLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKYTITPGTMYLLDGHEKHTMRIKEDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPESEPETV"
     gene            5509..6408
                     /gene="thpD"
                     /locus_tag="DKG71_RS11125"
                     /old_locus_tag="DKG71_11125"
     CDS             5509..6408
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606339.1"
                     /locus_tag="DKG71_RS11125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_11125"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_018091829.1"
                     /sec_met_domain="PhyH (E-value: 1.5e-37, bitscore: 128.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTAEVITPRKDPVVWSQPGTAGPFEPSELS
                     DFERDGFFAIGELLTAEEVAVYRAELDRLVLDPTMRADPRSIVEPKSQDVRTVFEVHKI
                     SEVFAKLVSDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYDTNGGLMIMPGSHEYFVGCEGATPKDNYKRSLQMQDAGIPSDEV
                     LTKMADKHSIRLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENAAVEPFA
                     APVRRPEYIGARDFTPVR"
     gene            complement(6496..7557)
                     /locus_tag="DKG71_RS11130"
                     /old_locus_tag="DKG71_11130"
     CDS             complement(6496..7557)
                     /NRPS_PKS="Domain: Aminotran_5 (16-348). E-value: 6.3e-22.
                     Score: 69.9. Matches aSDomain:
                     nrpspksdomains_DKG71_RS11130_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606338.1"
                     /locus_tag="DKG71_RS11130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_11130"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_026170007.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.9e-14, bitscore:
                     52.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHRREPLGGAEFAPETTYLNSASSGLLPRRAADALRAAIDESASY
                     GTMGRDYLAATTASRAAFARLMDLPAERVAVGSSVAVQSAFVAASLPEGAEVLAPEGDF
                     SSLVNPLAAHPGIKLRTVPLEALADAVRPGTALVAFSAVQSLDGRIADMAAVREAAAAH
                     GARTYLDITQAAGWLPLRAADFDFVVAGAFKWLLCPRGTTFMAFGGDFGRGEEWPAALH
                     AGWVAGEDIGESNYGLIQPAATARRFDEPHAHFSYVGAEQSLGLLGELGVSAIHAHDTA
                     LAEYYRAGLAERGLTPRPAPGSAIVSTPGIADAEHRLAEAGVRVSVRGGLLRAAFHLYN
                     STDDVDRLLALLG"
     aSDomain        complement(6514..7509)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DKG71_RS11130_Aminotran_5.1"
                     /evalue="6.30E-22"
                     /label="DKG71_RS11130_Aminotran_5.1"
                     /locus_tag="DKG71_RS11130"
                     /protein_end="348"
                     /protein_start="16"
                     /score="69.9"
                     /tool="antismash"
                     /translation="TYLNSASSGLLPRRAADALRAAIDESASYGTMGRDYLAATTASRA
                     AFARLMDLPAERVAVGSSVAVQSAFVAASLPEGAEVLAPEGDFSSLVNPLAAHPGIKLR
                     TVPLEALADAVRPGTALVAFSAVQSLDGRIADMAAVREAAAAHGARTYLDITQAAGWLP
                     LRAADFDFVVAGAFKWLLCPRGTTFMAFGGDFGRGEEWPAALHAGWVAGEDIGESNYGL
                     IQPAATARRFDEPHAHFSYVGAEQSLGLLGELGVSAIHAHDTALAEYYRAGLAERGLTP
                     RPAPGSAIVSTPGIADAEHRLAEAGVRVSVRGGLLRAAFHLYNSTDDVDRL"
     gene            complement(7672..8388)
                     /locus_tag="DKG71_RS11135"
                     /old_locus_tag="DKG71_11135"
     CDS             complement(7672..8388)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091831.1"
                     /locus_tag="DKG71_RS11135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_11135"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_109888950.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEAGLAAFAHRDDVEVVHRSFELDP
                     KAPAATDVPVLDMLATKYGVSRAQAEAMEARVADAAAGERLDYRSDRIHGNTFDLHRLL
                     HLGRAHGVQEALLDAFYRANFAEARLLGDPAVLREIAVRAGLPAEEVARVLADPGAYAR
                     EVRAEEREAAELGATGVPFFVIDRRYGISGGQPAEVFREALERAYADGAEPSQPAGDEA
                     GAAPVCGDDGCAVPGK"
     misc_feature    8992..9007
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 19.52"
                     /tool="antismash"
     misc_feature    9077..9097
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 21.41"
                     /tool="antismash"
     gene            9085..9861
                     /locus_tag="DKG71_RS11145"
                     /old_locus_tag="DKG71_11145"
     CDS             9085..9861
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263401.1"
                     /locus_tag="DKG71_RS11145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_11145"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_093642843.1"
                     /transl_table=11
                     /translation="MTTTAVVPNDVLRAVRLGLRMSQDELATALRRAGAELGEPNEASK
                     RLVQRWEAGVSRTPRGVYARALERVTGRPVEALGFSLPVPMARVHADGKGGHDMDAGGE
                     STAAVVDPQHAARPGAESFTGVWLSRYEFYSSSRDATYEGRHYVVIVQHGNRLTAQSLP
                     GASSNPDSPLTLDLTVDRNVITGSWVEQTAADGYYNGARYHGAVQMLVEPTGRRMTGKW
                     VGFGKEFDVNTGPWELRLVDRSTSKAALAQYSTPPE"
     misc_feature    9160..9162
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 gtcagccgcg gccgccctgc ggtgcggcgc tgcggcgctt gcgggcgaag accatcgacc
       61 cggcgcccgc ggcgagcagc agcgcggcgc cgccggcgag gtacggggtg gccgaactgc
      121 cgccggtctc cgcgaggttc tgggtgcggg gcccggagcc cgtcacctcg gtgtccttgc
      181 cgccggccgg ctcggtgccg ccgcccgtct ggccgccggt ggaaccgccg cccgtctggc
      241 cgccgctggt gccgccggag ccgccctgct cgggcgcctt ggcgcgcggc gtggtgcagg
      301 tggccttggc cagcgtgacc cggccctgca cgtcggcgac gttcagcttc agcgggttga
      361 gggagacgtt gaggtcgagc gcggtggccg cggcgccctt ggacgtgacg ttcttcttgg
      421 acaggtcgag ccgcacccgg cccaccccgg ggaccttgac gtccgtcgtg ccgcccgcgg
      481 tgagcgtgac cttcttgccc agcacccgca cgctgcccag cacattcgac tcggcccggg
      541 gccgggtgcc cgcctcgcag accgccttgg cggtgacctg ctggacctcg atcagggaca
      601 gcagcggcag accgggcaga tgcaccttcg cccgcacgac gttggcgtag ccctcggcct
      661 tgcggcggtc ggcggtggcc cgtgcggtgg cggcgtcggc gcgcagcacg ctgaacggcc
      721 tgccgtggtc gatgccgtcg agcttgacgg tgagcgcggt cttgctggcg gaggccgggg
      781 cgtgcacctc gttgagggcg acgttcagcg ggacgtcgag cgtcttgttg agcagcccga
      841 cgttcacgcc ggtacgcagc acgaccgcgt cggcggtgcc gcgggccggg ccatggtctc
      901 cggtggcatg cgcggggacg gcgccgagga ggacggcggg accggccgtg agcgcggtgg
      961 cgacaagggt cgcggcgagg cgtcggggca tgccgaagga gctggtggga ctggtggaca
     1021 cgtgtgtgga accctcacag gagaaatgga gccgccggcc gcgccaatgg gggacatcac
     1081 gcgggccgac ggcatcgacc cgcacatcgt gtccgtaccg agggtgaacg ggcagccacg
     1141 tgacgtcagt tcatcccaaa gggtggtttc cgtgccttta ttcgattcct ccgactcggg
     1201 gcaccacccg cgccacagcc gccccacctc ccgggtgacc cgcgttgacc gggcaggtcc
     1261 accggatcac cccggcggct accccacgat ctgccctcgc agcaccaccc ggcgcggtgc
     1321 ggccagcacc cggacgtcct cgcgcggatc gctctcgtag accaccaggt ccgccggggc
     1381 cccctcggtg aggccggggc ggccgagcca gtccctggcg ccccaggtgg tggccgagag
     1441 ggccgccacg gcgggcagcc cggcccgggt cagttcgacg acctcctcgg cgaccaggcc
     1501 gtgggcgagc gagccgccgg cgtccgtacc ggcgaagacg ggcaccccgg cgtcgtaggc
     1561 cgcccggacg gtgtcgtagc ggcggtcgtg caggcgccgc atgtggtccg cccagcgcgg
     1621 gaacttcgcc tcaccgccgt ccgcgagccg cgggaaggtg gcgatgttga cgagcgtggg
     1681 gacgatggcc actccgcgct cggcgaacag cgggatggtc tcctcggtga gcccggtggc
     1741 gtgctcgatg cagtcgatac cggcctcgac gagcggggcg agggtgtcct cggcgaagca
     1801 gtgggcggtc acccgggcgc ccagccggtg ggcctcggcg atggcggcct cgaccgctcc
     1861 ccggggccag caggcctcca gatcgcccgt ggagcggtcg atccagtccc cgacgagctt
     1921 gacccagccg tcgccgcggc gggcctccct ggcgacgtag gcgaccaggt cctccggctc
     1981 gatctcgtgc gcgtagttgc ggatgtagcg cttggtacgg gcgatgtggc ggccggcgcg
     2041 gatgatccgc ggcaggtcct cgcggtcgtc gacccagcgg gtgtcggagg gggatccggc
     2101 gtcgcggatc agcagggtgc cggcgtcacg gtcggtgagc gcctgcttct cgctggtggg
     2161 ggcgtccacc gggccgtgtg cgtccaggcc cacgtggcag tgggcgtcga ccaggccggg
     2221 cagggcccag cccgtcaccg tgatggcatc ccgtgccatc accggccggt cgaacgtcac
     2281 ccggccgccg accacccaca gctcgtcgcg cacctcgtcc ggtccgacga ggaccctccc
     2341 cttgaggtgc agcaccgcac cttcactcat ggacgtcata ccggcacttt aggcgtcgcg
     2401 ccggacaggg tgagcgggcg gtcacgccat ggatcggccg ctttccgcca gccgtttccc
     2461 cgggaacccg gcgtcccgcc ggactttcgg tccggacgga tatccgcgca ctctccgtag
     2521 agcttccgcc acccgcgccg cacagttctc cgtgcgttcc ccgccactct ccgtacattt
     2581 cccgcagcct tcccgcacat cgcctcgcgc atgttcttgc gggtcttgtc acggtcgcgc
     2641 cgcgcaggtt tccatgggcc ttccgccccg ccttccggcg gcgaattccc aattcctcag
     2701 aagtacagct tcccgtattc ttctgcccgc ttttccgcag ctcaaacgct aagattttgc
     2761 tagcggcaag ttccataatt cggacggatc tcatgggcgt tacggagctg tgaccgacta
     2821 cacaacgtgt ccgttttacc ccgaacctcc tggtcaaatg gggacatctc gcggtggcat
     2881 cgcgcacccg agcgcccccg cgcgataaca catcttgctc gcttccgtaa cccctgcccg
     2941 cgatgcaatt tcccttttcc ctgggtaaat ttaattccca tgaccgccgc acaagcagac
     3001 catgcaggtg cccgaagcga tatcagagaa tttccggagg gcttcaagct cgacacccca
     3061 cgcgtggagg acggagccgc gatctggcgc atcgcccgcg actcgaagac gctggatctc
     3121 aactcctcct acagctacct cctgtggtgt cgcgacttcg ccgccacctc cgtcgtcgcc
     3181 cgcgacacgg agggcgagcc ggccgccttc atcaccggat acctccgccc cgagcgcccg
     3241 gagaccctcg tcgtctggca ggtcgccgtc gacgacgcgc accgtgggca gggcctggcg
     3301 gccgcactcc tggacgggct gaccgcccgg gccagggacg agctgggcgt acgtttcgtc
     3361 gagaccaccg tgacccccga caacaccgca tccaaccggt tgttcgcgtc cttcgccgag
     3421 cggcactcag tgcccctcaa gagcgaggtg ctcttcgacg cggggctgtt cccggagcag
     3481 ggccacgagc cggaggtgct gcacctcatc ggcccgttcg agccaccggc cggcacgggg
     3541 cactcctgac gcacccagga ccgcgcggcg acgacgccgc agcggtcatg cgggacccgg
     3601 caccacaccc ccgtgacacc gccggtaccg cccagccccc tacagacttc tcatcctttc
     3661 gccactcatc tcccaggagc atgctgtgac catcacccag cccgatctga gcgtcttcga
     3721 gaccgtggag tcggaggtcc gcagctactg ccgtagctgg cccaccgtct tcgaccgcgc
     3781 gcagggcagc cgcatgaccg acgaggacgg ccacacctac ctcgacttct tcgccggggc
     3841 cggatcgctc aactacggcc acaacaaccc ggtcctcaaa cgcgccctga tcgactacat
     3901 cgagcgggac ggcgtcaccc acggcctgga catgtccacc acggccaagc gggcgttcct
     3961 ggagtcgttc cagaacatca tcctgcggcc gcgtgacctg ccctacaagg tcatgttccc
     4021 gggcccgacg ggcgccaact ccgtcgaggc cgcgctgaag ctcgcccgta aggtcaaggg
     4081 ccgcgagtcg atcgtgtcct tcaccaacgc cttccacggc atgtccctgg gcgcgctggc
     4141 cgtcaccggt aactccatga agcgcgccgg cgccggtatc ccgctggtgc acggcacccc
     4201 gatgccgttc gacaactacc tcgacggcac ctacccggac ttcctgtggt tcgagcggct
     4261 gctggaggac cagggctccg gtctgaacac ccccgccgcc gtgatcgtcg agacggtcca
     4321 gggcgagggc ggcatcaacg tcgcccgtcc cgagtggctg cgtgccctcg ccgacctgtg
     4381 tgagcgctgg gacatgctgc tcatcgtcga cgacatccag atgggttgcg gccgtacggg
     4441 tgccttcttc tccttcgagg aggcgggcat cgtgccggac atcgtcaccg tctcgaagtc
     4501 catcagcggc tacggcctgc cgctcgcgct caccctgttc aagcctgagc tggacatctg
     4561 ggagccgggc gagcacaacg gcaccttccg cggcaacaac ccggcgttcg tcaccgccgc
     4621 cgcgacgctg gacacctact gggccgacgg ccagatggag aagcagactc tcgcccgcgg
     4681 tgagatcgtc gaagcacacc tcaaggccat tgccgaggag cacccggacg ccatcgccga
     4741 gtaccgcggc cgcggtctgg tgtggggcat ggagctcaac gacaagccca ccgccgacaa
     4801 gattgccaag cgcgccttcg agctgggtct gctgatcgag acctccggcc cggagagcga
     4861 ggtcgtcaag ctgctgcccg cgctgacgac cacccccgag gagctggacg agggactccg
     4921 ggtcctcgcc cgcgcggtcc gcgactgcgt ctgaccactc cagagcccct taccgttcca
     4981 cagaaaggca ccgagtcacc gtgatcgtcc gatccttcaa ggacatcgaa ggcaccgacc
     5041 gccacgtcaa ggccaagtcc ggcacctggg agagcaagcg catcgtgctc gccaaggagc
     5101 gcgtcggttt ctcgctgcac gagaccaccc tgtacgccgg gaccgagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtactctgcg tagagggtga ggccgagctc acgaacgacg
     5221 agaccggcga gaagtacacc atcacgccgg gcacgatgta cctgctcgac gggcacgaga
     5281 agcacaccat gcggatcaag gaggacttcc gctgcgtgtg tgtcttcaac ccgcccgtca
     5341 ccggccgcga ggaccacgac gagaacgggg tctacccgct tctcaccgaa ccggagtctg
     5401 agcccgagac ggtctgaggc catccgcctg ccccgtccgg cagcgctgag gaaggcacgc
     5461 acgcgcacgt aagccagatt ccgtacgaga ggagaggaag gcaacaccat gaccaccgca
     5521 cccgagcgca ccgccgacct gtacccgacc cgtgggaccg ctgaggtcat caccccgcgg
     5581 aaggacccgg tggtgtggtc gcagcccgga acggccggcc ccttcgagcc gtccgagctg
     5641 agcgacttcg aacgcgacgg ctttttcgcc atcggggaac tgctcacggc ggaagaagtc
     5701 gcggtgtacc gcgccgaact ggaccgtctc gtcctcgacc cgacgatgcg cgccgacccg
     5761 cgctccatcg tcgagccgaa gtctcaggac gtacggaccg tgttcgaggt gcacaagatc
     5821 agcgaggtgt tcgccaagct ggtctccgac ccgcgcgtgg tcggccgtgc ccgtcagatt
     5881 ctcggctcgg acgtctatgt ccaccagtcg cggatcaatg tgaagccggg cttcggtgcc
     5941 tccggcttct actggcactc ggacttcgag acctggcacg ccgaggacgg actgccgaac
     6001 atgcgcaccg tgtcggtctc gatcgcgctg acggagaatt acgacaccaa cggcggcttg
     6061 atgatcatgc ccggctcgca cgagtacttc gtgggctgtg agggtgcgac gccgaaggac
     6121 aactacaagc ggtcgctgca gatgcaggat gcgggcatcc cgtcggacga ggtgctcacg
     6181 aagatggcgg acaagcacag catccgtctc ttcaccggta aggccggctc ggcgacgtgg
     6241 ttcgactgca acgccatgca cggctcgggc gacaacatca cgccctaccc gcgcagcaat
     6301 gtcttcatcg tcttcaacag cgtggagaac gctgcggtgg agccgttcgc ggcaccggtc
     6361 cggcgcccgg agtacatcgg ggcccgcgac ttcactccgg tgcgttaggg gacactcgca
     6421 ccggtggcgc ggccgccgca cagctggtct gtgcggcggc cgcgccgtgt tgtacggccg
     6481 gtaccgcgga ccgtcctacc ccagcagggc cagcaaccgg tccacgtcgt cggtcgagtt
     6541 gtagaggtgg aaggccgcgc ggagcagccc gccgcgcacc gagacccgca cccctgcctc
     6601 ggccagccgg tgctcggcgt ccgcgatccc gggggtggag acgatcgccg aaccgggcgc
     6661 gggccggggc gtcagaccgc gctcggccag ccccgcgcgg tagtactcgg cgagtgcggt
     6721 gtcgtgggcg tggatggcgg acacgcccag ctccccgagg agaccgaggg actgttcggc
     6781 gccgacgtag gagaagtggg catgcggctc gtcgaagcgc cgggcggtgg cggccggctg
     6841 gatcagcccg tagttggact cgccgatgtc ctcgccggcg acccagcccg catggagcgc
     6901 ggccggccac tcctcgcccc gcccgaagtc cccgccgaac gccatgaacg tcgtcccgcg
     6961 cgggcacagc agccacttga aggccccggc caccacgaag tcgaagtccg cggcgcgcag
     7021 cggcagccat ccggcggcct gggtgatgtc gaggtaggtg cgcgccccgt gggccgccgc
     7081 cgcctcgcgg accgccgcca tatcggcgat ccggccgtcc agcgactgca cggcgctgaa
     7141 ggcgaccagc gcggtgccgg ggcgcaccgc atcggcgagc gcctccagcg gcacggtacg
     7201 cagcttgatg ccggggtggg cggcgagcgg attgaccagg gagctgaagt cgccctcggg
     7261 ggccagcact tcggcgccct ccgggagcga ggccgccacg aacgcggact gcaccgcgac
     7321 cgagctgccg acggccaccc gctcggcggg gaggtccatc agccgggcga aggcggcgcg
     7381 ggaggccgtg gtggcggcga ggtagtcgcg gcccatcgtg ccgtaggagg cggactcgtc
     7441 gatggccgcg cgcagggcgt cggcggcgcg gcgcggaagg agtccggacg aggcggagtt
     7501 cagataggtc gtctcgggcg cgaattcggc cccgccgagt ggctcacgtc gatgcatgtc
     7561 cccactctgc tcggcacctc gcgccgcgtc catggcgact ttgctcggcc ggctcgcaac
     7621 ggttccttat cggcgcaggt cacaccgggt gccgccggac cggccccggt ctcacttccc
     7681 gggcacggcg catccgtcgt ccccgcacac cggggccgca ccggcctcgt caccggcggg
     7741 ctgtgacggc tcggcaccgt cggcgtacgc ccgctccagg gcctcccgga agacctccgc
     7801 gggctgcccg cccgagatgc cgtaccggcg gtcgatgacg aagaacggca cgcccgtggc
     7861 gcccagctcc gcggcctcgc gctcctcggc ccgtacctcc cgcgcatacg ccccggggtc
     7921 ggccagcacc cgggcgacct cctcggcggg cagccccgcg cggacggcga tctcacggag
     7981 cacggcgggg tcaccgagca gcctggcctc ggcgaagttg gcgcggtaga aggcgtcgag
     8041 cagcgcctcc tggacgccgt gcgccctgcc gaggtgcagc agccggtgca ggtcaaaggt
     8101 gttcccgtgg atccggtcgg agcggtagtc cagcctctcc cccgccgccg cgtcggcgac
     8161 ccgcgcctcc atcgcctcgg cctgcgcacg gctcacgccg tacttcgtgg cgagcatgtc
     8221 cagcaccggg acgtcggtgg cggccggcgc cttcggatcc agctcgaagg agcggtgcac
     8281 cacctcgaca tcgtcgcggt gcgcaaaggc ggcgagcccg gcctcgaagc gggccttgcc
     8341 gatgtagcac caggggcagg cgatgtcgga ccagatttcg acgcgcatga gctgactttc
     8401 ctcaaggtgg gtgggtggtg gtgaaacctt gggggtgggg gcggtattcc cgctcgggac
     8461 gcgcggcctt cgcgtaccgc ccggatcgag catgtgccga tcttgcgctt cctcgtggct
     8521 ctgtcgtggg ggacggggga agggcttcct gagcccgcac acagcggggt atccgcacgg
     8581 gggccagact cccgatgcct ccgggccgag ggacctgtca gcaccgtcgg ccgggtacgt
     8641 gaccgcggcc ccccaggtcc tgccggagtc gcgggagacg cgcacggtca tggtcatggg
     8701 cggttcccca cgtggtcacg gtggttccgg agccgcacgt tcgcatccgt cgcggcggac
     8761 agatcaccgc gttcgcgcgc ttccctgcgc tgcacggcga gggcggcgca gacggcgcag
     8821 ccctccacgg gggtgggttg gagctctggc agcgaaaggg aaaccgctgc gccgacaccg
     8881 cctccgggtg acccggaatt gccgttggcc atgtgctccg cctctcctcg accgtttctc
     8941 ggtgcttcga tggtcgcggc gtgcaaggga catcacgacg acgcttgggg cgtcaccggg
     9001 gcgtcactcg gaacggtgcc aacgacacct gggtgggcgt caaaccggtg tcattcgacg
     9061 gtgattcggt ctagctttga cgtcatgacg acgactgctg tggtgccgaa tgatgtgctg
     9121 cgagccgtgc ggctggggct gcgcatgagt caggacgagt tagcgaccgc gctacggcgc
     9181 gcgggcgccg agctgggaga gcccaacgag gcgtcaaaac gcttggtaca gcgctgggaa
     9241 gccggtgtca gcaggacgcc gaggggcgtg tacgcacggg ccctggagcg ggtgacgggc
     9301 cgccccgtgg aggccctcgg attctccctg ccggtaccga tggcacgggt tcacgcagac
     9361 gggaaaggag ggcacgacat ggatgccggg ggcgaaagca ccgctgcggt cgtggatccg
     9421 cagcatgccg cgcggccggg cgcggagtcg ttcaccggcg tgtggctcag ccggtacgag
     9481 ttctactcct ccagccggga cgccacgtac gagggcaggc actacgtggt gatcgtgcag
     9541 cacggaaacc ggctgaccgc ccagagcctg cccggtgcga gcagcaatcc ggacagcccg
     9601 ctcacactgg acctcaccgt ggaccggaac gtcatcaccg gttcatgggt ggagcagacg
     9661 gccgccgacg gctactacaa cggcgctcgc taccacggcg ccgtgcagat gctggtcgag
     9721 ccgaccggcc gccgcatgac cggcaagtgg gtcggcttcg gcaaggaatt cgatgtgaac
     9781 acgggtcctt gggaactgcg cctggtggac cgctcgacca gcaaggccgc gttggcgcag
     9841 tacagcactc cgcccgagtg accccgtagc tccgcggggt ggccggctgt ccggtgggta
     9901 cggtccccgc atgacctcgt cacagggatt tctcgggttc tgggagacga ccgggtccgc
     9961 caagaccttc actcatccgc tcgacccggc gctgctggat acgtatgtgc cgcggagcgc
    10021 gcgggtgctg gactacggct gtggttacgg gcggctgacc gccgagctgg ccgggctcgg
    10081 gtatggcgcg gtgtgtgggg tggatgtctc ggcggcgctg atcgcgcggg gggagcggga
    10141 gcatccggag ctggagctgc tgcgctgtcc cgggtttccg ctgccgttcg gggacggtgc
    10201 cttcgatgcg gcgctgctgt tcgcggtgct cacctgtgtt ccccgggacg cggaccagac
    10261 ggcgatcgcc ggggagctgg ggagggtggt gcggcccggt ggggtgctgt atctgagcga
    10321 tgtgccgttg cagggtgatg cgctcaaccg ggagcggtat gcgcggttcg cggaacggta
    10381 cgggacgtac ggggtgttcg agacgcccga cggcggg
//
