LOCUS       NZ_CP029541            10921 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. NEAU-S7GS2 chromosome, complete genome.
ACCESSION   NZ_CP029541
VERSION     NZ_CP029541.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NEAU-S7GS2
  ORGANISM  Streptomyces sp. NEAU-S7GS2
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Streptomyces sp. NEAU-S7GS2 isolated from saline-alkaline soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9641634)
  AUTHORS   Zhang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) School of Life Science, Northeast
            Agricultural University, Mucai Street, Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: AMRT Link v. v5.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003173275.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 05:28:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,458
            CDSs (total)                      :: 8,365
            Genes (coding)                    :: 8,052
            CDSs (with protein)               :: 8,052
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 313
            CDSs (without protein)            :: 313
            Pseudo Genes (ambiguous residues) :: 0 of 313
            Pseudo Genes (frameshifted)       :: 144 of 313
            Pseudo Genes (incomplete)         :: 230 of 313
            Pseudo Genes (internal stop)      :: 21 of 313
            Pseudo Genes (multiple problems)  :: 74 of 313
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:52:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 494277
            Orig. end                         :: 505198
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10921
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="5"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10921
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10921
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5921](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5921
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            <307..804
                     /locus_tag="DKG71_RS02285"
                     /old_locus_tag="DKG71_02285"
                     /pseudo=""
     CDS             <307..804
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558177.1"
                     /locus_tag="DKG71_RS02285"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_02285"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="GPLTGPSPTERGKSGSKIHLITDRNGLPLSLGISGANMHDSLRLK
                     PLARGIPPVRSRRDPRRRRPVMLHADKGYDYGHLRRWLSNRGIRHRIARKGIEPSTRAG
                     RHRWVVERTVSWLAGCRRLHRRCERQAEHILERKAAHFLAFVGTAAALITTADALLRDN
                     SG"
     gene            883..1272
                     /locus_tag="DKG71_RS02290"
                     /old_locus_tag="DKG71_02290"
     CDS             883..1272
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064180.1"
                     /locus_tag="DKG71_RS02290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_02290"
                     /product="VOC family protein"
                     /protein_id="WP_109886583.1"
                     /transl_table=11
                     /translation="MSVELNHTIVHARDNRESAQFFADLLGLEITAEWGPFIAVGLSNG
                     VTLDFATIPADHIAMQHYAFLVSEEEFDAAYARISECGIEHYADPHRQQPGAINHNDGG
                     RGVYFMDPAGHAMELITVPYGGWTS"
     gene            1836..2282
                     /locus_tag="DKG71_RS02295"
                     /old_locus_tag="DKG71_02295"
     CDS             1836..2282
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008739532.1"
                     /locus_tag="DKG71_RS02295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_02295"
                     /product="hypothetical protein"
                     /protein_id="WP_239471571.1"
                     /transl_table=11
                     /translation="MHTDLRHAAVVALGELARSHDYRDRADAGHGLAAFAEMQEALGPL
                     LELVLDSGDTFVTRRTTEALLRRKDRVGLTIVASALAVANSNHSDWTHTAIVDVFSIFT
                     SDRDNAIRLCEEMSQDTDKRVALGARQLHEVLAEIDPVLRTAWR"
     gene            2925..3878
                     /locus_tag="DKG71_RS02300"
                     /old_locus_tag="DKG71_02300"
     CDS             2925..3878
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971303.1"
                     /locus_tag="DKG71_RS02300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_02300"
                     /product="aminoglycoside phosphotransferase family protein"
                     /protein_id="WP_109886584.1"
                     /sec_met_domain="APH (E-value: 2.2e-23, bitscore: 82.4,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNHDEAAAVRPLTLAWVSRHLEVGERIVRTEVLHGGITAEMRRL
                     TIGRPDGGTRDLVLRTFVNVEDAEDWLNGEAGALTLLTGTGVPAPGLVAVDPTAAHCEY
                     PSLLMTHLAGRTVLDDEGLETRVPLLARQLVAIHALRPAERPREYVAWTTADTVVTPKG
                     ADAAAWAAAIDVIRRPAPRYEGRFLHRDFQPGNVLFDVSPPESAGARITGVVDWAATSW
                     GPADLDVAHCSTNLALLHGPAWGLRFAEAYEEAGGVLAAAASERLYWQVRDGLAFSEEV
                     QLVAQPWREAGRAELTTRAVEERLDAYVTALMDALG"
     gene            complement(4281..4901)
                     /locus_tag="DKG71_RS02305"
                     /old_locus_tag="DKG71_02305"
     CDS             complement(4281..4901)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006601757.1"
                     /locus_tag="DKG71_RS02305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_02305"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_093635850.1"
                     /transl_table=11
                     /translation="MYVQIRAETTRRFLLEAAATLFDEKGYAGTSISDISAHSGRTSGA
                     IYFHYASKEKLALAIVEEHFATWPHLISRYRQPGLPALEKLVGLSFEVARAFRDDIVVR
                     AGARLWAERKAINAQLPAPFLGWIEAVTQLLEEAARNGELAADVPPAATAHGIVCSFFG
                     LHTVSDALEGRRHIEEHLHDLWTLLLRGLQAEPDPAGLLGRVR"
     gene            5001..5921
                     /locus_tag="DKG71_RS02310"
                     /old_locus_tag="DKG71_02310"
     CDS             5001..5921
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543192.1"
                     /locus_tag="DKG71_RS02310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_02310"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_109886585.1"
                     /sec_met_domain="AfsA (E-value: 3.8e-15, bitscore: 54.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTSTVRAVGKSEPLSWSRTVERELVHRTSVAEVLLTDVRPTDTP
                     HAFLAAASWPRAHATFPRDGSQRHSPLILVETLRQLGLYIPLRFYAVPEVFRAVITDLY
                     FHLSPADELPARAGATEITCSARVSAVRRVPDESAVGLRLHVTFAACGVAFGQGGGGVR
                     FLDGARFTALRARSAVDAIPARPPEGWYHPPAAQLTVNHPHDAVLALDQGELLVSPADP
                     LHPFFFDHPTDHVPGMVLLEAARQAASYRSRGRLSRPLFGSLKASRFTELTPPARVLCT
                     RHGSTCAFRILQGGTETACGTFGYT"
     misc_feature    complement(5932..5947)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 19.79"
                     /tool="antismash"
     misc_feature    5975..5995
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.53"
                     /tool="antismash"
     misc_feature    complement(5976..5996)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.82"
                     /tool="antismash"
     gene            6146..6865
                     /locus_tag="DKG71_RS02315"
                     /old_locus_tag="DKG71_02315"
     CDS             6146..6865
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151397.1"
                     /locus_tag="DKG71_RS02315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_02315"
                     /product="HAD family phosphatase"
                     /protein_id="WP_109886586.1"
                     /sec_met_domain="HAD_2 (E-value: 4.9e-26, bitscore: 90.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTADSILSWTPAAVVFDCDGTLMDTERHWEDARDMVLHSYGCAPS
                     GEFAEKARGLHYTECGRLMADVAGRPELTGDMTESLLKHFRELVAADPTTMPGAPELVH
                     STVQFAPLAVASNCPLEVVESCLGMAGLRDCFSHIVVPNDTTRPKPHPDVYLTAAQLCG
                     VEPADTLAVEDSHCGIQAAAGAGMRVLGVGPRPSDRETALADWWVHSLAEPAVQGWADA
                     RIPAQGTGPTGPGTTTS"
     misc_feature    6332..6334
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            7153..7299
                     /locus_tag="DKG71_RS42530"
     CDS             7153..7299
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DKG71_RS42530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_162596810.1"
                     /transl_table=11
                     /translation="MRVSISRPPGATAPHLPFSFTTVPHSRAPRLQWRGSLLLERCATP
                     SMT"
     gene            7601..8305
                     /locus_tag="DKG71_RS02320"
                     /old_locus_tag="DKG71_02320"
     CDS             7601..8305
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /locus_tag="DKG71_RS02320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_02320"
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="WP_093635856.1"
                     /transl_table=11
                     /translation="MSPLDISLADLIARLDEELPDANELARISEARLRAQTLSDLGDQL
                     IDHYVSKAKQTGASWTEIGDAIGVSKQAAQQRHAPGPFERYTNLNRHSIVLAQEAARTH
                     KHDSIGTEHLLLGLLGEPRGLAYEVLIAKTKSEQRIRDAIEEAMPPAGQKALRGHIAFR
                     PESKEAIDQARRSATDLGHDWVGTEHALLGLIHTEESPAAQILHNLGFTPDELRETIKT
                     EITKRSAARDKQ"
     gene            complement(8625..9242)
                     /locus_tag="DKG71_RS02325"
                     /old_locus_tag="DKG71_02325"
     CDS             complement(8625..9242)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508301.1"
                     /locus_tag="DKG71_RS02325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_02325"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_109886587.1"
                     /transl_table=11
                     /translation="MSDGNRRVEAAEALQDRARRLLPDHPVRIALLDLIAETGTLTSTE
                     AAARLGHSSGLCSFHLRQLARYGLIEEAPHHGGRARPWRLRWDTPQHTGEEEPEEFTAL
                     ARGLEDESYQHWLAHRDQAPAEWQQDESFSTVLHLTPAETAELATSIRRLFAGYRNRGD
                     HPATRSPNAVAVAAVTRLFPLLTEHQVPPQSTPSHVEGSTDS"
     misc_feature    9236..9250
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 21.51"
                     /tool="antismash"
     misc_feature    9237..9249
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 19.6"
                     /tool="antismash"
     misc_feature    9261..9277
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.7"
                     /tool="antismash"
     gene            9344..10003
                     /gene="def"
                     /locus_tag="DKG71_RS02330"
                     /old_locus_tag="DKG71_02330"
     CDS             9344..10003
                     /EC_number="3.5.1.88"
                     /GO_function="GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /GO_process="GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="def"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1275: peptide deformylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952030.1"
                     /locus_tag="DKG71_RS02330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_02330"
                     /product="peptide deformylase"
                     /protein_id="WP_204165810.1"
                     /transl_table=11
                     /translation="MAVGFGDTVRDRRVRVQGKPVESYPRLAPEVERGAVRRITVVGEE
                     ILRRRCRKVTEFGTAELAKLIDDMFATNQVAEGAGLAANQVDVDLQLFVWDITDEWGVR
                     HVGHIANPVLEEVSADDRHLVEEPEGCLSVPGPYRVVPRLDRAVVRGVDKDGNPLVIEG
                     RGYFARCLQHETDHLHGHLYLDRLAQRERKAALQEMQDSQDKVFARRASAAKRLGK"
     gene            complement(10329..10904)
                     /locus_tag="DKG71_RS02335"
                     /old_locus_tag="DKG71_02335"
     CDS             complement(10329..10904)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737369.1"
                     /locus_tag="DKG71_RS02335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DKG71_02335"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_093635860.1"
                     /transl_table=11
                     /translation="MTEPSGRRERKKAATRQKIADTALRLFLERGYDAVGIRDVAAEAD
                     VAVTTVFAHFASKEALVFERDQDFEQRLTRAVTDRPPHEPLIPALHREVQALVRHCTAE
                     GSAPVRRMIEESPALREYEESMSLRHAQALAAALAADPHLSRSATACRATARFAIDAYA
                     LACRADDPGATVDEVFRMIKAAWEATAP"
ORIGIN
        1 tggcggtgaa gcgggccgcg gcaacgatgt tgaaagtgtc gtcgtccagg ctgctgtcgg
       61 cgaccaggag gtcatcagtc tctgtaacgg acgcaccgct catgctccga tgcagatgac
      121 aggcatgttc cgccagattc tgctccatcc tgtccgtcaa gtcggcgtcg tcaaggaaat
      181 ggacattcac aacgaatgat cccagcatga ctgggcgcag gttgcacgtg cttttggggc
      241 gctcaccgcc cgtgtcacgt cggcgaccag cacattcgat acacgcccta agcgcgaaca
      301 cggtgggggc cactgaccgg accgagtccg accgaacgcg gcaagagcgg atcgaaaatc
      361 cacctgatca cggaccggaa cggattgccc ctgtcgctgg gcatctccgg tgccaacatg
      421 cacgacagcc tccgcctgaa gccactggcg cgggggatcc cgcccgtccg ctcccgacgc
      481 gacccgcgtc gtcggcgccc agtcatgctc catgcggaca agggctacga ctacggccac
      541 ctgcgcagat ggctcagtaa caggggcatc cgccaccgca ttgcccgtaa gggcatcgag
      601 ccctccacac gagcgggccg ccaccgctgg gtcgtcgaga ggacggtctc ctggctggcc
      661 ggatgccggc gcctgcatcg ccgctgcgaa cgccaggccg agcacatcct cgaacgcaag
      721 gccgcgcact tcctcgcctt cgtcggtaca gccgcagctc tcatcactac cgccgacgca
      781 cttcttcgtg acaacagcgg ctaggatcta ccgggacatc gcgtgtcgat caccggctgg
      841 gtgaggcatg gaggtgtcgg gagatgcctt cggaggcatt ccttgtcagt tgagttgaac
      901 cacacgatcg tccacgcccg ggacaaccgg gagtccgccc agttcttcgc ggacctgctg
      961 ggccttgaga tcaccgcgga gtggggcccg ttcatcgcgg tcggcctgag caacggcgtc
     1021 acgctggact tcgccactat ccccgcggac cacatcgcca tgcagcacta cgccttcctg
     1081 gtctccgagg aggagttcga cgcggcgtac gcgcggatca gcgagtgcgg catcgagcac
     1141 tacgccgacc cgcaccggca acagcccggc gcgatcaacc acaacgacgg cggccgcggg
     1201 gtgtacttca tggacccggc gggccatgcc atggagctga tcaccgtgcc gtacggcggc
     1261 tggacctcgt aaccacatgc gccgagggcc gcaccccagc ccggggtgcg gccctcaatc
     1321 gccccttgtg ctgatgtact cgcggccggc gccctgcgat cacccgcccc accgcccggc
     1381 ggggagcttg ccctccgtga ctggcaccga acaccggcag agaacccata agccatcacc
     1441 gacaccaaag gaaacgtgtg acgacattcc ggagcccgtt tcaaccacct tgctgctgtc
     1501 tgatcccaac gcaccctcgt ccgcccctgt ttcgcaatgg ttgatctcat aagtcgacac
     1561 ctcacccggg aatcgcccct gtactggcgc gcgcgacccc tgaagcccga accaaacacc
     1621 cccgccggaa taaaaagctc ttttaagtga cgaccgaggg gccaatgcgt ggatgacttt
     1681 gcgcgaaagt ccgtcgtcca cgcataggga cgtcctgata cggccgtcag gccgccggcc
     1741 acacaggcac cgtgcccttg gaccgcgtcc ttgcgatctc ctcataaggt gcctcgacgg
     1801 tacattgacc ggagtcatgg gagggaggca aacaggtgca cactgacctg cggcatgcag
     1861 ctgtcgtggc actgggcgag ctcgcgcgaa gtcatgacta ccgtgaccgg gcggacgccg
     1921 gtcacggcct ggcagcgttc gccgagatgc aggaggccct cgggccgctg ctggagcttg
     1981 tgctcgactc aggtgacacc ttcgtcaccc ggcggaccac ggaagcattg cttcggcgaa
     2041 aggacagagt cgggctgaca atagtcgcgt ccgcactggc agtcgccaac tcgaatcaca
     2101 gcgattggac ccacaccgca atcgtcgatg tattcagcat cttcaccagc gaccgggaca
     2161 atgcgatacg gctgtgtgag gagatgtcgc aagacaccga caaacgcgtt gcgctggggg
     2221 cacgtcaatt gcatgaggtc ctggcagaga tcgatcccgt tcttcgtacc gcatggcgct
     2281 aggccgacgc tttcctgaga gctcatgtgc tcgacgacaa aggaacctcg ccgacgggcc
     2341 tcatcagcgg cccgtcgggc gcctcactgg cgtcacctcg acggcctccg cgccctcagg
     2401 ggcgccctgt tcggagggga cttcgtcgtc atgacgctgc gccgcgcacg gcctcgtgct
     2461 ctctgcgcga ggtccagaag caggtcgtcg accatgaaga gcccggcgtc ggacgccgga
     2521 gccagcatgc gccacgacgg tcaacacgca gcccggccag gctgccccgc tttcaatcga
     2581 gactggctcg acttccaggg cgacttcgtg cgtgaggtcc tcggggccga tcggggcgct
     2641 cactcgttct cctcggtgat gtcagcgatg aggccgtgcc ctgtgcattt ccccccgctc
     2701 gcagttactt cgccaccgcc tgtgacggct cccggcgatc actgccggtg cccgctgacg
     2761 gggtctttcc aggctgtggc cacggctcga tgcgggtgcc tgaacggtgt ctgcagcagc
     2821 tgcgagacgt gccgttgaac gggcacgagg aatagagcag ggttgccatc gccttcaacg
     2881 aaaggctttt gcgcgtgccg tcgtccggat gatggggtgg cacggtgacc aaccacgacg
     2941 aggcggcggc tgtccgaccg ttgacactgg cttgggtgag ccggcacctg gaggtcggcg
     3001 aacggatcgt cagaaccgag gtgctgcacg gcggcatcac cgccgaaatg cggcggctga
     3061 ccatcggcag accggacgga ggcacccgtg acctggtact gcggaccttc gtcaacgtgg
     3121 aggatgccga ggactggctg aacggggagg ccggcgccct gaccctgctc acggggaccg
     3181 gcgtgccggc tcctggactg gtcgcggttg atccgaccgc cgcgcattgc gagtatccat
     3241 cgctccttat gacacacctg gcgggccgga cggtcctcga cgatgaggga ttggagacgc
     3301 gtgttcctct gctggcccgt caactcgtgg cgatccacgc gttgcgaccc gccgagcggc
     3361 cccgggaata tgtggcgtgg acgaccgccg acaccgtcgt gactccgaag ggcgccgacg
     3421 cggcggcatg ggccgcggcg atcgacgtga tccgcaggcc cgcgccgcgt tatgaagggc
     3481 gattcctgca ccgggacttc caacccggca acgtgctgtt cgacgtgtcg cccccagagt
     3541 cggcaggtgc ccggatcacc ggcgtcgtcg actgggcagc gacctcttgg ggcccggcgg
     3601 atctcgatgt ggcgcactgc tcgaccaatc tcgcgctgct gcacggcccg gcgtggggcc
     3661 tgcggttcgc tgaggcgtat gaggaggccg gcggggtgct ggccgcggcc gcgagcgagc
     3721 ggctttactg gcaggtgcgg gacgggctgg cgttctcgga agaagtgcag ttggtggcgc
     3781 agccgtggcg ggaggcaggg agggcagagc tgacgacgcg agccgtggag gagcggctgg
     3841 atgcctatgt caccgccctg atggacgccc tgggctgagc caagacggtc cgggacgcgg
     3901 gcacggcggc gaacggccat tgcctgtgcc ctacctcgat tcgaacagcg acatcgcccg
     3961 ccgtgaccac tccccgtgat ctgtgacaga acctcactct cacctacaaa gccgcccgtg
     4021 ccgtcctcgt ccgtcgacga ccacctcgac accgcacgcc agcggccagg ccgaggccgg
     4081 ggcggaggcg ggacaacccc gtggaccgcg caaaggaccg ccgcagcacg ggcgctctca
     4141 ccgggcatcc cgcgctgcac gcccggctgc acggccacgc cgctccccga cgatccccgc
     4201 ggcccgtcca cttgaacagg cgcagctgca gactgcggcc ttcctcaacg tctgcaagat
     4261 cggtgggcgt cgcgcgccgc tcagcgcacg cgaccgagca gaccggcggg atcaggctcg
     4321 gcctgaaggc cgcggagcag cagagtccac aggtcatgca ggtgctcctc gatgtgccgg
     4381 cgtccctcca gggcgtcgga gacggtgtgc agaccgaaga aggagcagac gatgccgtgg
     4441 gcggtggcgg caggtggcac atcggcagcg agttcaccgt tacgggcggc ttcttcgagt
     4501 agctgtgtaa cggcttcgat ccagccgagg aacggtgcgg gaagctgggc gttgatggct
     4561 ttgcgttcgg cccaaaggcg tgctccggcc cggacgacga tgtcgtctcg aaaggcgcgt
     4621 gccacctcga agctcagccc gacgagtttc tccaaggccg gcagaccggg ctgccggtag
     4681 cggctgatca aatgcggcca cgtggcgaaa tgttcctcga cgatggcgag ggccagcttc
     4741 tccttgctgg cgtagtggaa atagatggcc ccgctggtgc gaccggaatg cgcactgatg
     4801 tcgctgatgc tggtaccggc gtaccctttc tcgtcgaaca aggttgccgc cgcctcaaga
     4861 aggaacctgc gcgtcgtttc cgcccggatc tgcacgtaca cctcaaacgt tgttcccgtt
     4921 ccgccggaag gaaacctacc gctccccgcg ccccggaatt caagggtgcg catgttcgaa
     4981 tttcggtatt ccctggtcgt atgagcacct caacggtcag agccgtcgga aagagtgagc
     5041 cgctcagctg gtcccgtacc gtcgagcggg agctggtgca ccggacctcg gtggcagagg
     5101 tcctccttac ggatgtacgg ccgaccgata ccccgcatgc cttcctggcg gcggcgtcat
     5161 ggccgcgcgc gcatgcgacg ttcccccgcg acgggtcgca gcggcacagc ccgctgattc
     5221 tggtggaaac actgcgtcag ctcggtctct acattccctt gcggttctac gcggttccgg
     5281 aggtcttccg tgcggtcatc accgacctgt acttccacct cagccccgcc gacgagttgc
     5341 cggcccgcgc cggagcgacc gaaatcacct gctcggcacg ggtcagcgcc gtacggcggg
     5401 tgccggacga gtccgccgtc gggctgcgac tgcatgtcac cttcgccgcg tgcggggtgg
     5461 ctttcggcca aggtggcggg ggcgtgcgat tcctggacgg cgcgcgcttc accgcgctgc
     5521 gcgccaggag cgcggtcgat gccatcccgg ctcgccctcc ggagggctgg taccacccgc
     5581 cggccgcaca gctgaccgtg aatcacccgc atgatgcggt cctcgctctc gaccagggcg
     5641 agctgctggt ctcgccggcc gacccgctgc acccgttctt cttcgaccac cccaccgatc
     5701 acgtgcctgg catggtgctg ttggaagccg cccgccaggc cgcctcgtac cgcagtcggg
     5761 gcaggctgag ccggcccctt ttcggcagcc tgaaggcctc ccggttcacc gaactgaccc
     5821 cacccgcacg cgtgctgtgc acccggcacg gctcgacgtg tgcattccgc atcctccagg
     5881 gcggcacaga gacggcttgc ggcaccttcg gttatacgta gagcacacaa tattgacaat
     5941 tgtgaattgg ttgacctttc tgcgttgcca tccgtaacgt aacgatcgct atgttccggc
     6001 tggagcggca gaactcctct ccatgggagc tgccctgccg gaatccctcg gcccggtgcc
     6061 gggcgtccgt gaaagcgacg ctgccctgcc tctcttgcga ccggtcgctt cggcgccttc
     6121 gggccccatc agaacggact cccgaatgac cgcagacagc atcctttcct ggacccccgc
     6181 ggccgtcgtt ttcgactgtg acggcacgtt gatggacacc gaacgccact gggaggacgc
     6241 ccgtgacatg gtgctgcaca gctacggctg cgcaccctcg ggcgaattcg cggaaaaggc
     6301 acgagggctg cactacaccg aatgcgggcg cttaatggcc gatgtggccg ggcgaccgga
     6361 actgaccggg gacatgacgg aatcattgct caagcacttc cgtgaactcg tcgccgcaga
     6421 ccccaccacc atgcccgggg cgccggaatt ggtgcattcg accgtgcagt tcgccccgct
     6481 ggccgtggcc agcaactgcc cgcttgaagt cgtcgagtcc tgcctgggaa tggctggact
     6541 ccgggattgc ttcagccaca ttgtggtacc caacgacacc acgagaccca aacctcatcc
     6601 ggacgtctac ctcaccgccg cccaactctg tggggtagag cccgctgaca cgctggccgt
     6661 tgaggactcc cactgcggaa tccaagctgc cgcaggcgcg ggcatgcggg tactcggagt
     6721 cggtccccgc cccagcgaca gggagacggc gttggccgac tggtgggtcc actcgctggc
     6781 agagccggcg gtgcaggggt gggccgacgc ccgcatccct gcccagggca ctggtcccac
     6841 tgggcccggg accacgacgt catgacgtca tgacggcagc ccggcccgcg gcgcacttca
     6901 taacggcccg cgggtcgggt ggcattcagg gtgacctgcg gtgcgggcgc caccgccagg
     6961 tgtccggcat cccgcgacac cgatggggcg acgccgttca actggctcat caggtcaact
     7021 ggctcttcag gccctaccag gggccggcat ggcccgaggc gtcgctacgg gaaatcgccg
     7081 tgggggcccc tggccacggc cccgggacga cgtgcggaac cggctgccgg ggtagaagga
     7141 tccggtgccc cgatgcgcgt cagcatctcc cgaccgccgg gggcgaccgc gccgcacctt
     7201 cccttctcgt tcaccacggt gccgcactcg cgggcgccgc ggctccagtg gcgagggtcg
     7261 ttgctgctgg agcggtgcgc cacgccctcc atgacctgac catggcattc atacccacgg
     7321 aggattcgct cgattccgcc cgagacggtg gacggtgggc agcaacggcg ccacaagttc
     7381 ccacagacca tcaggcacca ggtcggcagc catcacgcga agaatcttgt cggatcgatc
     7441 cccaactgcc aagctccaca aggcaattcc gaagcgatcc agcgggtcgc gatcaggccc
     7501 gagccgcacc gggccatcgc acgacactgc ggagcagagc cagaaaaacg atcgtcaatt
     7561 acaggttgac gacccttgat cgtcaactta aggttgacgc atgagtccac tcgacatcag
     7621 ccttgccgac ctgatcgccc ggctcgacga ggaactcccc gacgccaacg aactggcccg
     7681 catcagcgag gcgcgactgc gcgcccagac cctgtccgac ctcggtgacc agctcatcga
     7741 ccactacgtc agcaaggcca agcagaccgg cgcgtcgtgg accgagatcg gcgacgccat
     7801 cggggtgtcc aagcaggccg cccagcaacg ccacgcaccc ggccccttcg agcggtacac
     7861 caacctgaac cgccacagca tcgtgctggc acaggaggcc gcccgaacgc acaagcacga
     7921 ctccatcggc accgaacacc tcctgctcgg cctgctcgga gaaccccggg gcctggcata
     7981 cgaggtactg atcgcgaaaa ccaagtcgga gcaacgcatc cgcgacgcaa tcgaggaagc
     8041 gatgccgcca gccggacaga aggccctgcg aggacacatc gcgttccggc cggagagcaa
     8101 ggaagccatc gaccaggcac gccgctcagc gaccgacctc ggccacgact gggtcggcac
     8161 ggaacacgca ctgctgggcc tgatccacac cgaggagagc ccagccgcgc agatcctgca
     8221 caacctcggc ttcacgccgg acgaactgcg cgagacgatc aagaccgaga tcaccaaacg
     8281 atccgccgca cgcgacaagc agtagcgaaa cgaccaatca ccgcgcagga ccgggcggca
     8341 cagccttccc ccctcgacac gggacggtgc gctcgtgccg ccaaccccca cccgacgtcg
     8401 ccaaaaacct tgtagtgcac ggtgagtgac aggcagccgt gcactacgac tcatttcgaa
     8461 accgtcagta agaggtcagc aagcgccagc atcgctccac accagctcac cgctgaagag
     8521 gagttgtccg aggtgtgcag catctttcgg tgtggctttg tgtacttgaa agggctctgg
     8581 tcccgcgccg ccccggcttc gggatggaca ttaattgatc ttgctcagga atcggtcgat
     8641 ccctcaacgt ggcttggcgt ggactgcggt ggcacctgat gttcggtgag gagcgggaac
     8701 agtcgggtga ctgcggctac agccacggca ttcggtgagc gagtggcggg gtgatccccc
     8761 cggttgcggt agccggcgaa caagcggcgg atggaggtgg cgagttcggc tgtctcggcc
     8821 ggggtgaggt ggagcacggt gctgaaggat tcgtcctgct gccactccgc cggcgcctgg
     8881 tcccggtgag ccagccagtg ctggtagctc tcgtcctcca gcccgcgggc gagggcggtg
     8941 aattcttccg gctcttcctc gcccgtgtgt tgcggagtgt cccatcgcag cctccacggc
     9001 cgggcccggc ccccatggtg aggcgcttct tcgatgaggc cgtaccgggc gagttggcgc
     9061 agatggaagg agcacaggcc ggaactgtgc cccaggcggg cggcggcctc ggtggaggtg
     9121 agggtgccgg tttcggcgat caggtcgagc agggcaattc gtacggggtg gtcgggcagg
     9181 agccgccggg cccggtcctg cagggcctcc gcggcttcga ctcgacggtt tccatcactc
     9241 attttgcaaa gtctgctact ttgcaaagca cttggcaagc cgtcaggtct ctcggcggct
     9301 gggtgccggt ggctaggcgc tgcgtagaag ggattgatgg ttcatggctg ttgggttcgg
     9361 tgacacggtg cgggatcgcc gggtgcgtgt gcagggcaag cctgtcgagt cctatccacg
     9421 gctcgcgccg gaggtggagc gtggtgcggt gcgcaggatc accgtggtcg gggaggagat
     9481 actgcgccgc cgttgccgga aggtgactga gttcggcacg gcggagctgg cgaagctgat
     9541 cgatgacatg ttcgccacca accaggtggc ggagggggct gggctcgccg ccaaccaggt
     9601 ggatgtggat ctgcagttgt tcgtgtggga catcacggat gagtggggtg tgcgtcacgt
     9661 tgggcatatc gccaacccgg ttcttgagga agtatccgcc gacgaccgtc acctggtgga
     9721 ggaacccgaa ggctgcctgt cagtccccgg cccttaccgc gtggtccctc gcctcgaccg
     9781 tgctgtcgtc cggggcgtcg acaaggacgg caatcctctg gtgatcgagg gcagggggta
     9841 cttcgcccgg tgtctgcagc acgaaaccga tcacctgcac ggccacctgt atctggaccg
     9901 gctcgcacag cgggagcgga aagctgctct ccaggagatg caggactccc aggacaaggt
     9961 gttcgcccgg cgcgcctcgg cggcgaagcg gctcggcaag tgactggcgt tctctgaagt
    10021 tcagttgagg cgtgctgggg aggacgacgt cccgtagcta gggcgctggt gaaggcccag
    10081 gactgctccg ccctgcccga tacggccggg ttgggccgta cggctcaacc cggctcccct
    10141 cccccactgc cgcggtgccc gtgcccgcct aggagctgtc cggccgatca tgtggggagc
    10201 cagatgacga gggctgccgc gggccgcccg ccgcggcagc cctcccgcag ggggcggcct
    10261 gactgtcggt cttctccggg atcgtgtgcc ggcgccggcc ccggccgcag cgcgccggcg
    10321 tccccggctc agggcgcggt ggcttcccag gccgccttga tcatccggaa gacctcgtcc
    10381 accgtggccc cgggatcgtc cgcccggcag gccagcgcgt aggcgtcgat ggcgaaccgc
    10441 gcggtcgccc ggcaggccgt cgcactccgg gacaggtggg gatcggcggc cagcgccgct
    10501 gccagcgcct gcgcgtgacg caggctcatc gactcctcgt attcccgcag ggcgggtgac
    10561 tcctcgatca tgcgtcggac gggggcgctg ccctccgccg tgcagtgccg caccagggcc
    10621 tggacctcgc ggtgcagcgc cgggatgagc ggctcgtgcg gaggccggtc ggttaccgcc
    10681 ctcgtgaggc gctgctcgaa gtcctggtcg cgctcgaaca ccagggcctc ctttgaggcg
    10741 aagtgggcga agacggtggt gacggccacg tcggcctcgg ccgccacgtc acggatgccc
    10801 acggcgtcgt acccgcgctc caggaagagc cgcagcgcgg tgtcagcgat cttctgacgg
    10861 gtcgcggcct tcttgcgttc gcggcgtccg gacggctcgg tcatgccctg acgctaccag
    10921 g
//
