LOCUS       NZ_CP068988            11360 bp    DNA     circular CON 16-APR-2024
DEFINITION  Bacillus paralicheniformis strain SUBG0010 chromosome, complete
            genome.
ACCESSION   NZ_CP068988
VERSION     NZ_CP068988.1
KEYWORDS    RefSeq.
SOURCE      Bacillus paralicheniformis
  ORGANISM  Bacillus paralicheniformis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 4317835)
  AUTHORS   Bhatt,H.D., Bhatt,P.P., Patel,J.H. and Thaker,V.S.
  TITLE     Characterization of Bacillus species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4317835)
  AUTHORS   Bhatt,H.D., Bhatt,P.P., Patel,J.H. and Thaker,V.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2021) Department of Biosciences, Saurashtra
            University, University Road, Rajkot, Gujarat 360005, India
REFERENCE   3  (bases 1 to 4317835)
  AUTHORS   Bhatt,H.D., Patel,J.H. and Thaker,V.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) Biosciences, Saurashtra University,
            Kalawadroad, Rajkot, Guajarat 360 005, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2019
            Assembly Method                   :: CLC NGS Cell v. 11.0
            Assembly Name                     :: PMBP_Rhizobaci_2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: NZ_CP023665
            Genome Coverage                   :: 75.0x
            Sequencing Technology             :: IonTorrent
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003171815.2-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 07:39:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,350
            CDSs (total)                      :: 4,240
            Genes (coding)                    :: 4,181
            CDSs (with protein)               :: 4,181
            Genes (RNA)                       :: 110
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 30 of 59
            Pseudo Genes (incomplete)         :: 44 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 20 of 59
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:25:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2838485
            Orig. end                         :: 2849845
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP068988.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11360
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="11"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11360
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11360
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6360](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6360)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    128..142
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.94"
                     /tool="antismash"
     gene            complement(157..864)
                     /locus_tag="DI291_RS14290"
                     /old_locus_tag="DI291_14640"
     CDS             complement(157..864)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183759.1"
                     /locus_tag="DI291_RS14290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DI291_14640"
                     /product="DNA alkylation repair protein"
                     /protein_id="WP_128822637.1"
                     /transl_table=11
                     /translation="MDFETVMKELEALGTENMKKRYITNGAHEPLFGVATGKMKPLAKK
                     IKKNQPLAEQLYATGNYDAMYFAGIIADPITMTEADFERWIDEAYFYMLSDYVVAVTLA
                     ETDFAQAVADKWISSGEELKMSAGWSCYCWLLGNRPDDEFNVTKIAGMLDQVEHAIHDS
                     PDRTKSAMNNFIYTAAISYLPLHEKAVETAKAVGPVELRRDKKKSSILNASKNIQMDVE
                     KGRLGFKRKYVRC"
     gene            complement(1267..1890)
                     /locus_tag="DI291_RS14295"
                     /old_locus_tag="DI291_0475"
     CDS             complement(1267..1890)
                     /EC_number="2.-.-.-"
                     /GO_function="GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /GO_function="GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328063.1"
                     /locus_tag="DI291_RS14295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DI291_0475"
                     /product="L,D-transpeptidase"
                     /protein_id="WP_023855307.1"
                     /transl_table=11
                     /translation="MKHKRRIGLLIVASVSLVGTMAACSKSKAEMEQVNAEVNQPADTS
                     STETSKNVKTKTEKKEIDWTKPSDGKYPDIKNNDSIWIDVSTKKQEAYIKDGSKTIYTM
                     NISSGLDQTEDDSTPKGTYYVEPERGEWFFSPKYQEGAEYWVSWKNHGEFLFHSVPFNK
                     DKKVIEKEAKKLGKKASHGCIRLAIPDAKWIYENIPEKTKVVIS"
     gene            complement(2128..2670)
                     /locus_tag="DI291_RS14300"
                     /old_locus_tag="DI291_0480"
     CDS             complement(2128..2670)
                     /EC_number="2.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /locus_tag="DI291_RS14300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DI291_0480"
                     /product="GNAT family protein"
                     /protein_id="WP_023855308.1"
                     /transl_table=11
                     /translation="MKTIQVSETVMLREFKTEDAESLFELIDRSRNHLIEWLPWVDGVK
                     SVKDSQGFITFAQEEAVSGSSLHLGIFEKGRLAGKVCLYQINDRIKTASIGYYIGADFE
                     GRGLVTQAVKQLAAFAFQTRGLNRLEIKMISQNKRSLAVPQRLRFSYEGCLRESGEFQG
                     KPADQLIFSLLKREFQS"
     gene            complement(2648..3223)
                     /locus_tag="DI291_RS14305"
                     /old_locus_tag="DI291_0485"
     CDS             complement(2648..3223)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /locus_tag="DI291_RS14305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DI291_0485"
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="WP_049936996.1"
                     /transl_table=11
                     /translation="MSANVLGLLGSLRYPSKTEEALRICLKAAERRGCTTSIFDGRITP
                     LEFCDGRDEEDTYVEGVALLREKVREADAIVIASPEYSGSYSAFAKNMIDFLGPDYLQG
                     KWAGVIGVAAGNSAANTVAHLSAILARNGCWLVPTPVELPASHTIFSQHASSPTAKHLV
                     SLLNALGENIAFSVNQINRRQYENHTSQ"
     gene            complement(3213..3989)
                     /locus_tag="DI291_RS14310"
                     /old_locus_tag="DI291_0490"
     CDS             complement(3213..3989)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DI291_RS14310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DI291_0490"
                     /product="hypothetical protein"
                     /protein_id="WP_128822640.1"
                     /transl_table=11
                     /translation="MTHYIFPHHMIPFLGSDQQAYLMNEEGEIMKVGFLNERSNMYWGR
                     LKPGASIQLPDEETELIHVMNEISQREEFISPSIHMVTDDSFSKKLEAKFEQSGFFISS
                     TNQKGIHIVFSGFFNCDSFQRFSDDAQENQLHWLPLYFDGRIARVGPLLGPYLQFEDLR
                     KRLLAAAYFPDVTDSLWKAAQTAPFSEHHLPLVHLEWLLTQISWIVHDYSSANQLRLHM
                     HQISLDPANMETKRHPILKVPSPIPSHERKDECNVR"
     gene            4161..4304
                     /locus_tag="DI291_RS14315"
     CDS             4161..4304
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DI291_RS14315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_161513139.1"
                     /transl_table=11
                     /translation="MSKQVSEASKTLLEVLDEGRTFAVDPHHHALVHNPFVWEEEKDNE
                     EN"
     gene            complement(4360..4953)
                     /locus_tag="DI291_RS14320"
                     /old_locus_tag="DI291_0500"
     CDS             complement(4360..4953)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /locus_tag="DI291_RS14320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DI291_0500"
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_023855312.1"
                     /transl_table=11
                     /translation="MPFLCETAPVRTYARNQIVPLENSRIYIVQSGLVFIWETHLDGRQ
                     VLVNILDGNSAIQIHNFVHASYEMQIKKTSFLQITFLEAIDNQMNILHALRKQTFMSEE
                     LAAIHREKYVEHRLYFFLQFLKRHFGKEIKKEHYLIAVPITHEELASAVNSTRPTITKL
                     LTKMIDKNQVVYQQNGKNRWIILKPLFFSAAEPF"
     gene            complement(5001..6360)
                     /locus_tag="DI291_RS14330"
                     /old_locus_tag="DI291_0510"
                     /pseudo=""
     CDS             complement(5001..6360)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="DI291_RS14330"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DI291_0510"
                     /product="YcaO-like family protein"
                     /pseudo=""
                     /sec_met_domain="YcaO (E-value: 6.2e-50, bitscore: 168.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLPLDGQLLIDKRTGIINGLQKVDIEPVLPRKAVSWRAQVTNGR
                     ILANIPSDRIAGGTHFSNDQARNAAIGEAIERYSGNYKGGPFIRGSYQELKRKGYKLLD
                     PDRIPLYSTWQYAEKGFPFIPFTKDLKVNWVQGFSLTCKEEVLLPASLVYINYYEGIFA
                     NEPPVHFVNYAGIASGPTLEFALKSGLLELIERDATAIWWGCRGQIDAIDINDHPDLLN
                     SLEAREESEIRYTFLPLTTDINIPVIGAMLYDPVRQIACMGFRAGPIRKSLAEGCCRNG
                     SILSFIIGFTGSG"
     gene            complement(6326..7918)
                     /locus_tag="DI291_RS14335"
                     /old_locus_tag="DI291_0515"
     CDS             complement(6326..7918)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /locus_tag="DI291_RS14335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DI291_0515"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_023855314.1"
                     /transl_table=11
                     /translation="MISFRWEVAAEDGAMLCADAYVPAETGAFPVILTRTPYGKSAFSE
                     TAKAWCELGVAFVAQDVRGRYQSGGRWIPYSAEAKDGAATLKALKRASWCNGHIALMGE
                     SYGAFTAFLAAQSEIGRQTPSALITLVPAMGLKKTAFTSDRVFYLGDRLWWDASFGSSR
                     ENIEHLFSAVLNKHSSAVFTLPVIDIPTVFPMQLANWRRILENEHNEESLNLESVHQPC
                     LHIGGWYDPFVSHTIHNFKTLRKKEKRQALVIGPWSHEVNRIPAFEILHDQKHAVIPLG
                     SLEHAWLESIWSDKLYPFPEILLYVTGADQWIAPEEWPPAGTMDFTLYLRECRLSTDMP
                     AGGENTSSSYTYDPLQPVPSQEYPSKRNEIEKRADVLVFTSAPFVRDIILSGAPAVKLW
                     VSSTAPSTDFIVVITEVYPDGTSVYLTHGHIRTGDEKGGIRPQDVTISMEPRCHKIKNG
                     RRIRLSISSSSFPKYARHLNRNGDQHLQSSPQIAEQTIWHSEHMLSHLKIPIENSLLER
                     CSYEFTPGWPTAH"
     misc_feature    complement(7911..7922)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.25"
                     /tool="antismash"
     gene            8103..8981
                     /locus_tag="DI291_RS14340"
                     /old_locus_tag="DI291_0520"
     CDS             8103..8981
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1033: iron
                     compound ABC transporter, periplasmic"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /locus_tag="DI291_RS14340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DI291_0520"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_128822648.1"
                     /transl_table=11
                     /translation="MKKAIFLIAMFIVILSSCSSQPENSDTSHNKGSQKKEMSYTDDTG
                     HKISFSKTPKKIICLTEICSDTLHELGLKPEAVLEDGLVSQDEFYGKDHQIPLIGGTFY
                     EPNLQNIYQYEPDLVIGLGGVHDNLRDGLGDKAPLFLVNPNHYQDSLSFVETFGSMLGE
                     EKKAKQLTNDFKKKLEQVKKMSPKNKKALVMYGSDINFGIDTQSSIVGSILNEITDYPW
                     KVKGKQEGHNAGGTKYSLEEILKENPDVIFVETFNFGNNKGPSLSEQLANNRIWKRLKA
                     VKNDNVHEVRT"
     gene            9082..9729
                     /locus_tag="DI291_RS14345"
                     /old_locus_tag="DI291_0525"
     CDS             9082..9729
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /locus_tag="DI291_RS14345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DI291_0525"
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="WP_128822650.1"
                     /transl_table=11
                     /translation="MGVKHKRLLILYLVLALSCIYLFYLSLSAGSPSASFSSVFRILTK
                     GGESDPLLYVSIMEIRMPRAMIAFFIGMMFALSGTILQDTFHNGLAGPELLGVSAGASL
                     TMAVLTVLHLPVAFYLHPFISLAGGLAGGIVVILCTMGKANKQNMILIGAAVSAFFQSL
                     IIITISIGSQNDIGLLFFYLIGSLANRNWDHVIEIAPWFLVCVPALWPWQSR"
     gene            9714..10085
                     /locus_tag="DI291_RS14350"
                     /old_locus_tag="DI291_0530"
     CDS             9714..10085
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /locus_tag="DI291_RS14350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DI291_0530"
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="WP_128822652.1"
                     /transl_table=11
                     /translation="MAKPLNVLRLGDEAAQGRGVSVVKMRTFLLFLSVSLSAAGVAVCG
                     PVGYIAFIAPHLARRIMGTSNAFVILPLSGLIGSCTLMGTDLSARMLFYPQELPVGLFT
                     VMFGGLLLLFFLNKRRKST"
     gene            10082..11098
                     /locus_tag="DI291_RS14355"
                     /old_locus_tag="DI291_0535"
     CDS             10082..11098
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /locus_tag="DI291_RS14355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DI291_0535"
                     /product="iron ABC transporter permease"
                     /protein_id="WP_051303761.1"
                     /transl_table=11
                     /translation="MNSRNRFVMLLFLNVFFLIVFFSLSMILENNASPLSVISDMINGT
                     APAYRYEVIQDLKIPRGLTAILAGGMLGLAGAMIQSLSRNPLAEPGLIGVTAGGVCAAV
                     LWITFGPAHLQLSFFLPFFACFGGILAAFFVYVLSSYTKKMSSVFLLNGILISAVLQAV
                     TSFILLRHQEALGSILLWTIGSLNGRVWTHLNLLWPIAVPALIAGLLTAGIANAWRLGT
                     ESAVLLGLSLKKTNALLFFISALLTAGAVSIVGAIGFIGLIGPHIAKRIVGEDARKSFP
                     ISMLLSSLLLLTADLIAQYIFSFLPFSFISQNIQLPAGAVTVIMGVPFFLYLLRKQT"
ORIGIN
        1 attgactatt tctcaattcc tgtcttactt cattcacgat cacttttgct aattcaattc
       61 tttcttctgt atcatatcgt tttgccatct gttcaaaaac gttatcttcc atgttccact
      121 ccctatttat ttttagaaat ttcactgcag actgtgctaa catcttacat atttccgttt
      181 gaaaccaagg cggcctttct caacatccat ttgaatattt ttggaagcgt tcaggatact
      241 gctttttttc ttgtcccgcc gaagttcgac cgggcctact gcttttgcgg tttcaaccgc
      301 cttttcatga agcggcaaat atgaaatcgc ggctgtgtag ataaagttat tcatagccga
      361 ttttgttcga tccggagaat cgtgaatcgc atgttccacc tgatcaagca taccggcaat
      421 tttggtcaca ttaaattcat cgtccggtcg attacctaaa agccagcagt aacagctcca
      481 gcctgccgac attttaagct cttcaccgct tgagatccat ttatcagcaa cagcctgcgc
      541 aaaatcggtt tctgccaaag ttacggcaac aacataatca gatagcatat agaaatacgc
      601 ctcatcgatc cagcgctcaa aatccgcttc agtcattgtg attgggtcag caataatgcc
      661 ggcaaaatac atggcatcgt agttcccggt agcgtaaagc tgctccgcca aaggctgatt
      721 tttcttgatt ttctttgcga gcggcttcat tttgcctgtc gccacaccaa aaagcggttc
      781 gtgtgcacca ttcgttatgt atcttttttt catattttcc gtacccagcg cttcaagttc
      841 cttcataacc gtttcaaaat ccatgctttc acttccttta taaattaaat tcatttcttc
      901 aattccatta tcatactctt tcactttatc taatcatttc aaatcaactt catttgtttt
      961 taatgctctc cataaacagc tgcatcagca tcaaaagatc cgttgatctt ttttagccgc
     1021 tgtctgatca actgtccttt tacactctga cagggttttg cgttaacggt tttccttttg
     1081 tttcctaagt ccaaagaaaa ctaaatttcc tctatgttta ttatatgaaa cattctaaaa
     1141 agcagaaagg ggtctatttt tgaccgcctt tctgcttcat gcgtcaaatt cggatgccac
     1201 cgatatttgc aaaaagaaga ctcttaaaaa aagagtctcc taacttgata aataaaacga
     1261 gccaaatcag ctgatcacta cttttgtttt ttcaggtatg ttttcataaa tccattttgc
     1321 atctggaatc gcaagcctga tgcatccatg ggaagctttt ttccctaatt tttttgcttc
     1381 tttttcaatg acttttttat ctttgttgaa tggtacgcta tgaaataaga attcaccatg
     1441 atttttccat gaaacccaat actctgcacc ttcctgatat tttggggaga aaaaccactc
     1501 acctcgttca ggctctacat aatatgtacc ctttggtgtt gaatcatcct ctgtttgatc
     1561 gagacctgac gaaatattca tcgtatagat tgttttactt ccatctttta tgtaggcctc
     1621 ctgttttttt gtagacacat caatccagat ggaatcatta tttttaatgt cagggtattt
     1681 tccgtctgaa ggttttgtcc agtcgatttc ttttttctcc gtttttgttt ttacattctt
     1741 cgatgtttct gttgatgacg tatcagcagg ctggtttact tccgcgttca cctgctccat
     1801 ttcagctttt gactttgaac aagctgccat tgtgccaacc agagatacag aagctactat
     1861 caataatcca attcgccgtt tatgcttcat aagaagtctt tccccctata taaatcttaa
     1921 acattacgat ttggattgta gcttagttca tccttttttg tcaaagcaca aggccattca
     1981 aaaagctcag gcttcattca aactcggagc atcttcattt ccacgcttct ctaaaactcg
     2041 ttttaatccc agcactgccg gcacttcagt caattgttgt cgtggtcatt tttaaacgtt
     2101 agctaaaatc tttctttttc cgcatcctta tgattgaaat tcccttttta gcaacgaaaa
     2161 tataagctgg tcagcaggct ttccctgaaa ttctccgctt tctcgcaaac agccctcata
     2221 agagaaacga agtctctgag gtacagcgag gcttcgttta ttttggctga tcatttttat
     2281 ttctaagcga tttagccctc gcgtctgaaa ggcaaaagca gccagctgtt ttacagcttg
     2341 tgtcacgagt cctcttccct caaaatccgc acctatatag tagccaatag aggctgtttt
     2401 gattctgtca ttgatttgat ataagcacac tttccctgcc aaacggcctt tttcaaaaat
     2461 gccgagatgc agcgaagatc cggataccgc ttcttcttgt gcaaacgtaa tgaacccttg
     2521 actgtctttc acacttttta ccccgtcaac ccaaggaagc cattctatca aatgatttct
     2581 ggatcgatca atcaactcaa acaggctctc agcatcttct gtcttgaatt ctcttaacat
     2641 aacggtctca ctgacttgta tggttttcat attgcctccg atttatttgg ttaacgctga
     2701 aagcgatatt ttcgcccagt gcattcagca aacttaccaa atgctttgcg gtcggcgaag
     2761 acgcgtgctg tgaaaagatg gtatggcttg ccggcagttc tacaggggtg gggacgagcc
     2821 agcatccgtt tcgtgcaaga attgctgaaa gatgcgctac cgtattggcc gctgaatttc
     2881 cggctgccac ccctataaca cccgcccatt ttccctgcag ataatccggc ccgagaaaat
     2941 caatcatatt tttggcaaaa gcagaatagc ttccgctgta ttccggagac gcgatgacaa
     3001 ttgcatccgc ttcccgcact ttttccctca acaaagccac cccttccaca tatgtatcct
     3061 cttcatcgcg tccgtcacaa aattcaagcg gcgtgatcct gccatcaaat atacttgtgg
     3121 tacaacctct gcgctctgcg gccttcaggc atatccttaa agcttcttct gtttttgacg
     3181 gatagcggag acttccgaga agtcccagca cattagcgga cattacattc atcctttctt
     3241 tcatgacttg gaataggaga cggcactttt aatatcggat ggcgttttgt ttccatgttt
     3301 gcggggtcaa ggctgatctg atgcatatgc agccggagtt gatttgcaga gctgtaatca
     3361 tgaacgatcc agctgatttg cgtcagcagc cattccagat ggacaagagg gaggtgatgt
     3421 tctgaaaagg gagccgtttg cgctgccttc cataaagaat cagtgacatc gggaaaatat
     3481 gctgccgcta atagccgctt ccttaaatct tcaaattgaa gataaggacc aagcaatgga
     3541 cctactctgg caatacgtcc gtcgaaataa agcggcagcc aatgcagctg gttttcttgg
     3601 gcatcatcac tgaatctttg aaaagaatcg caattgaaaa atccgctgaa tacaatgtga
     3661 atcccctttt ggtttgtcga cgatataaaa aatccggatt gttcaaattt cgcctcaagc
     3721 ttttttgaaa aagaatcgtc agtaaccata tgaatagagg gagatatgaa ttcctctctt
     3781 tgagaaattt cattcatgac atggatcagt tctgtctctt catccggcaa ttgaatcgaa
     3841 gcgccaggtt ttaaacgtcc ccagtacatg ttagaacgtt cattcagaaa tcctactttc
     3901 atgatttctc cttcttcatt catcaaatag gcttgttgat cggaacctag aaacggaatc
     3961 atatgatgag gaaatatata gtgtgtcatc agatcaccct tttttctatc atcttaaata
     4021 aaattcgctg tcaaagctgt gtttctccat accattctgt cgtcattgat ctgacagaaa
     4081 tacgttgcca attgaaagaa aataaagatg agatcaagca tgcatgcaat ctcaaaaatt
     4141 tttcaaagag gtggagaaaa atgagcaaac aagtttcaga agccagcaaa acgttattgg
     4201 aagttttaga tgaaggcaga acatttgctg tagatccaca ccatcatgca ctggttcaca
     4261 atcctttcgt ttgggaagaa gaaaaagaca acgaagaaaa ttaataaaag ataaaaaaag
     4321 ctgccgtcaa tttgaagttg acgcagcttt tttttatctt tagaacggct ctgcagcaga
     4381 aaagaacagc ggctttaata tgatccagcg atttttcccg ttttgctgat agacaacctg
     4441 atttttatca atcattttgg ttaacaattt ggtaatggtc ggccttgttg aatttacagc
     4501 ggatgcaagc tcttcatgtg tgataggcac tgctattaaa taatgctctt ttttgatttc
     4561 ctttccaaaa tgtcttttta gaaattgaag aaaaaaatac agcctatgct ctacatattt
     4621 ttccctgtgg atcgctgcaa gctcttctga cataaatgtt tgttttctca atgcatggag
     4681 tatattcatt tgattatcaa tcgcttcaag aaatgtaatt tgcagaaagg acgttttttt
     4741 gatttgcatt tcatatgagg catggacaaa gttatgaatt tgaatggccg aatttccgtc
     4801 aaggatattc accaaaactt gacgtccgtc aagatgtgtt tcccatataa aaacaaggcc
     4861 tgattgaaca atgtaaatcc ggctgttttc caaaggaacg atttgatttc gcgcataggt
     4921 tctgacaggg gcggtctcac ataaaaaagg caatgaaaaa cacctctttt tataaaggtc
     4981 aatatcatcc ctcttcatga ttagctgtga ggaatgggct tcaaattgat atgatgctcg
     5041 tccagcacat gttctgacca ccctatgcgg tacggcactt catacagacg tttaggcgca
     5101 aggtatggaa aggctgccgg tgcattaggc acaagcccgg gagcgatcac tcgtgttact
     5161 gtgaaaccgg cagttctgac atcttcagtc gtcacatctg cataaaaaca ctcgatttga
     5221 tgttttgcca gagcctctgc aatatctgat gaagtaagag actgcttttc cggaagcatt
     5281 cccttttctt ctgcatcaaa gatatggcga atatcctgat gtgagtcagg atctaaataa
     5341 tactggctat tatgcaaaag atcaatcata tcatggtaat ccgatttaaa tgactgaaca
     5401 tatttcctgt cttttcgata ggaatgcagt gcagcctgat gcagtattcc tgtatgaacc
     5461 gcgtcccata acgggctatc cggatccagt aaatccaatg ataaatgata ggattgaacc
     5521 gtttctgcag cagccttcag caaggctttt tcggatcggg cctgctcgaa aacccataca
     5581 tgcaatttga cgaacagggt catacagcat cgccccaatg actggaatat ttatatctgt
     5641 tgttaaagga agaaaagtat agcgtatttc actttcttct cttgcttcca aactgttaag
     5701 aagatcggga tgatcattta tgtcaatggc atcaatctgg cccctgcatc cccaccagat
     5761 tgcggttgcg tccctctcta tcagttccaa aaggcctgat ttaagtgcaa attccaaggt
     5821 cggcccgctg gcaatgccgg cataattcac aaaatgaaca ggcggctcat tggcaaatat
     5881 cccttcataa tagttaatat aaacaagcga ggcgggcagc agcacctctt ctttacatgt
     5941 aagagaaaaa ccttgtaccc aattcacttt taaatccttt gtaaacggga taaaaggaaa
     6001 acctttttca gcatactgcc aagtagaata aagaggaatc cggtcagggt ccagcagctt
     6061 gtagcccttt cttttcaact cctgatagga gcctcgaata aagggccctc ctttataatt
     6121 tccggaatat cgctcaatcg cttccccgat tgctgcattc cttgcttgat cattggaaaa
     6181 atgagtgccc cccgcaatgc gatctgacgg aatattagca aggatccttc cattggtgac
     6241 ttgcgctctc catgagacgg ctttcctcgg taaaacaggt tcaatatcca ctttttgcaa
     6301 accattaata ataccggttc ttttatcaat gagcagttgg ccatccaggg gtaaactcat
     6361 atgaacacct ctctaacaat gaattttcta ttggtatttt gagatgtgac agcatgtgtt
     6421 cagaatgcca aatcgtctgt tcagcaattt ggggtgaaga ctgaaggtgt tgatctccat
     6481 ttcgattaag gtgcctggcg tatttcggaa atgaactgct cgatatcgaa agacgaatcc
     6541 ggcggccatt ttttattttg tggcatctcg gttccatcga tatcgtcacg tcttgcggtc
     6601 tgatacctcc tttctcatcg cctgttctta tatggccatg tgttaaatat actgacgttc
     6661 catccggata aacttcagtt atcacaacaa taaaatcggt tgaaggagcg gtagagctga
     6721 cccataattt tacggccgga gcaccggaca aaatgatgtc acgaacaaag ggggctgagg
     6781 taaatactaa tacatctgcc cttttttcaa tttcatttct tttagaagga tattcttggg
     6841 aaggaacagg ctgtaaagga tcgtaagtgt aagaggaact tgtgttttct ccccctgccg
     6901 gcatatcagt cgacaaccgg cattcgcgta aatataaagt gaaatccatt gtaccggctg
     6961 gcggccattc ctctggtgct atccattgat cagctcctgt cacatacagc aggatttcag
     7021 gaaatggata cagcttgtca gaccatatac tctcaagcca tgcatgttca agcgagccaa
     7081 gaggaatcac ggcatgtttt tgatcatgta agatttcaaa ggctggaatc cggttcactt
     7141 catggctcca cgggccaatc accagcgcct gacgcttctc tttttttctt aaggttttaa
     7201 aattatgaat ggtgtgggat acaaatggat cgtaccatcc tccaatatgt aaacaaggct
     7261 gatggacaga ctccaagttt aacgattctt cattgtgctc attctctaaa atccggcgcc
     7321 agttggcaag ctgcatggga aagactgttg gaatgtcaat caccggcaat gtgaaaacag
     7381 cggaagaatg cttgttcaat acggctgaaa acagatgctc tatattttct cgtgaagaac
     7441 cgaatgaggc atcccaccaa agccggtcgc cgagataaaa aacgcgatca gaagtgaatg
     7501 cagttttttt cagtcccatt gcaggcacga gtgtaattaa agctgacggc gtttgcctgc
     7561 caatttccga ttgagcagct aaaaaagctg taaacgctcc ataagattca cccattaatg
     7621 caatatgtcc gttgcaccac gaagccctct tcaacgcttt aagcgttgct gcaccatcct
     7681 ttgcttctgc agaatacggt atccatcttc cgcctgactg atagcgccct cttacatctt
     7741 gcgctacaaa cgcgacacct aattcacacc acgctttagc tgtctcggaa aatgctgatt
     7801 tgccataggg agttctcgtg agtataactg gaaatgcacc ggtttcagcc ggtacataag
     7861 catctgcgca aagcatggct ccatcctctg cagccacctc ccatcgaaaa gaaatcattt
     7921 tcgccctcct ttccatttgt cattcatcca acaatcaaat cgaaatggtt acgatttgaa
     7981 actcatcata atttttattc ctttatatgt caagaatttt tattttttcc atacttgcca
     8041 acgttgtaaa atgttattaa acttataaat agtaacaatt acgatttgaa aggggcatcc
     8101 atatgaagaa agccattttc ttgattgcca tgttcatcgt tatcctttct tcctgctcaa
     8161 gccagccgga gaacagtgat actagccata acaaaggcag tcaaaagaaa gaaatgtcgt
     8221 atacagatga cacgggccat aaaatttctt tctcgaaaac acctaaaaag attatttgtt
     8281 taacagaaat ttgcagtgat acattgcatg aattaggttt gaagcctgaa gctgtgctcg
     8341 aagacggact tgtcagccaa gacgaatttt atggaaaaga ccatcaaatt ccattaatcg
     8401 gcggcacgtt ttatgaacct aaccttcaga acatttatca atatgaaccg gatttggtga
     8461 ttgggcttgg cggtgttcat gataatttaa gagacggcct gggcgataaa gctcctctct
     8521 tcttagtcaa tcccaatcat tatcaagact cgctttcttt cgttgaaaca tttggttcga
     8581 tgcttggtga agaaaagaaa gcaaaacagc tgacaaatga ctttaaaaag aaattggagc
     8641 aagttaaaaa gatgtcgccg aaaaataaaa aggctttagt tatgtacggg tctgatatta
     8701 acttcggcat tgatacacaa agttccattg tcggttctat tttaaatgaa ataactgatt
     8761 acccgtggaa agtcaaagga aagcaagaag gacacaatgc cggtggaacc aaatactcgc
     8821 tggaagaaat cttaaaggaa aatcccgatg tcatctttgt cgaaaccttc aatttcggaa
     8881 ataataaagg cccttcatta tcagagcagc ttgctaataa ccgcatttgg aaacgattga
     8941 aagccgtgaa aaatgacaac gtccatgaag tccggacttg atttgggcaa atggtcgggg
     9001 tgtcggttcg ttaacaatta ttctccgtga agcaatggaa caattatatc caacgattga
     9061 tgttgaaaag gaataaaata aatgggcgtg aagcataaac gattattgat tttatatctc
     9121 gtccttgctt tatcatgtat atacctattc tatctttccc tttcagcagg ttctccttcc
     9181 gcttctttca gcagtgtgtt tcgcatattg acaaagggcg gagagagtga cccgctgctt
     9241 tacgtttcca tcatggagat tcggatgccg cgggcaatga tcgccttttt cataggaatg
     9301 atgttcgcgc tgagcggaac tatcctgcag gatactttcc ataatggtct ggctggtcct
     9361 gaactattag gtgttagtgc gggcgcttca ctcacaatgg cagtgttgac tgttctgcat
     9421 ttgcctgtag cgttttatct ccatccgttt ataagcctgg ctggaggact ggctggaggg
     9481 atcgttgtta ttttatgcac gatgggaaaa gccaataaac aaaatatgat tttaatcggc
     9541 gctgcagtca gtgccttttt ccaaagttta atcattatca ccatcagtat cggcagtcaa
     9601 aatgatatcg gtttgctatt tttttatttg ataggcagtc ttgccaacag aaattgggat
     9661 cacgtgatag aaatcgcccc ctggtttctt gtttgtgtac cggcgctttg gccatggcaa
     9721 agccgttgaa tgttcttaga ctcggagatg aagcggccca aggcagaggc gtttcagtcg
     9781 tgaaaatgcg tacttttctt ttatttctca gtgtttcgct ttcagccgcc ggcgttgccg
     9841 tatgcggccc tgttggatac atagcattta ttgcgcctca cttagcgaga cgcataatgg
     9901 gaaccagcaa tgcttttgtc attttaccgc tgtcagggtt gatcggaagc tgcacgttaa
     9961 tgggaaccga tttgagtgca agaatgttgt tttatccgca agaactgccg gtcggattgt
    10021 ttaccgtaat gtttggcggt ttattgctct tgttcttttt aaataaaagg aggaagagca
    10081 catgaactct agaaaccgat ttgtcatgct gctttttttg aacgtattct ttttaatcgt
    10141 gtttttttcg ctttccatga tcctggagaa caacgcatcc cccctgtctg tcatttctga
    10201 catgatcaat ggaactgctc ctgcttaccg gtatgaagtg atccaagatc tgaaaattcc
    10261 gaggggatta actgcgatat tggcaggcgg aatgcttggg cttgcaggcg ctatgattca
    10321 atccttatcg agaaatccat tggctgaacc ggggctgata ggtgttacag ctggcggagt
    10381 ttgcgctgct gttttatgga ttacatttgg tcctgctcat ttgcagcttt cattttttct
    10441 gccattcttt gcatgcttcg gcggaatttt ggcggccttt ttcgtttatg tattaagctc
    10501 ttataccaag aagatgtcgt ctgtcttttt gttaaacggc attttaattt cagcggtatt
    10561 gcaagctgtg acttcattta ttttgctaag acaccaagaa gcgcttggaa gcattttatt
    10621 gtggacaatc ggatcattga atggaagggt ttggactcac ttgaatcttt tatggccaat
    10681 tgctgttcct gcgctcattg cgggattgtt gacagcggga atagcaaatg cctggaggct
    10741 gggaacagaa tctgctgtgc tcctgggcct ttctttaaag aaaacaaacg ctcttttatt
    10801 tttcatctct gcccttttaa cagcaggagc ggtttcgatt gttggcgcaa ttggctttat
    10861 cggtttaatc ggccctcaca ttgccaaacg gatcgttggc gaggatgcga gaaaaagctt
    10921 tcctatcagt atgctgctat cttctctttt gctgttaaca gccgatttaa ttgcacagta
    10981 tatattttca ttcttgcctt tttctttcat cagccaaaac atccagcttc ccgcaggagc
    11041 cgtaaccgtc attatgggcg tgcctttttt tctttattta ttaagaaaac aaacatgata
    11101 aaagcgaggt gctatcaatc ataccatgga tgccattacg atcgaaaacc tgtctgtatc
    11161 aatctcaaat aacaacatta ttcgcaatct gtctgtgaac atccccaaca atgaaataac
    11221 cgggatactt ggcacaaatg gttcggggaa gtccaccctt ctccaaacga tatccaaatt
    11281 gctgaaaccc gattctggac gaatttctat tcacggaaag ccgcttacca gctattcggc
    11341 aaagcagctg gcacagactg
//
