LOCUS       NZ_QGHB01000025        10819 bp    DNA     linear   CON 09-DEC-2024
DEFINITION  Lentzea atacamensis strain DSM 45480 Ga0215650_125, whole genome
            shotgun sequence.
ACCESSION   NZ_QGHB01000025
VERSION     NZ_QGHB01000025.1
KEYWORDS    WGS; RefSeq.
SOURCE      Lentzea atacamensis
  ORGANISM  Lentzea atacamensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Lentzea.
REFERENCE   1  (bases 1 to 69345)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing
            the most valuable type-strain genomes for metagenomic binning,
            comparative biology and taxonomic classification
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 69345)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2018) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Expected Final Version            :: No
            Genome Coverage                   :: 90.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003148865.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 16:35:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,333
            CDSs (total)                      :: 9,254
            Genes (coding)                    :: 9,110
            CDSs (with protein)               :: 9,110
            Genes (RNA)                       :: 79
            rRNAs                             :: 6, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 6 (5S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 32 of 144
            Pseudo Genes (incomplete)         :: 121 of 144
            Pseudo Genes (internal stop)      :: 2 of 144
            Pseudo Genes (multiple problems)  :: 11 of 144
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:50:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 34661
            Orig. end                         :: 45480
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QGHB01000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10819
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10819
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10819
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5819](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5819)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(248..259)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     gene            658..1350
                     /gene="hemQ"
                     /locus_tag="C8D88_RS44735"
                     /old_locus_tag="C8D88_12538"
     CDS             658..1350
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="C8D88_RS44735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D88_12538"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_109642599.1"
                     /transl_table=11
                     /translation="MARLNYNELNDTIRYTMWSVFRVEPGKLPEDRSAAASETQNYLDA
                     LEGKGVVVRGVYDVAGLRADADFMIWWHAEEIEQVQAAYTGFRRTPLGKVSTPVWSQVA
                     LHRPAEFNRSHIPAFLANEEPRKYVCVYPFVRSYEWYLLPEEDRRKMLADHGKEARDFP
                     DVRANTVASFALGDYEWMLAFEADELHRIVDLMRHLRGTEARLHVREEIPFYTGTRVPA
                     AELVTNLP"
     gene            complement(1398..4472)
                     /locus_tag="C8D88_RS44740"
                     /old_locus_tag="C8D88_12539"
     CDS             complement(1398..4472)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012311.5"
                     /locus_tag="C8D88_RS44740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D88_12539"
                     /product="S8 family serine peptidase"
                     /protein_id="WP_109642601.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.8e-62, bitscore:
                     203.4, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRGNVKVLGAAFAALALTAGTATAASAAPQPTGPQRAVDEVTLVT
                     GDKVLLGQAQQVVAIQPGEGRKGMTFKTHRGADGHTMVVPTDAFGPLAAGRLDRRLFDV
                     TTLLEFGYRDRMPLIVDGQARLGARSSADWAALRAEAASGDKIWLDGIRKPSLDRSVAQ
                     IGAPAAHQRGITGKGVKVAIVDTGVDENHPDLKGQQIAEKNFTSDPDTTDRVGHGTHVG
                     ATVASAGSQYGGVAPGAQLLDAKVCVEFGCQESWILDGMRWAAEQGAQVVNMSLGGGDT
                     PEIDPLEQAVNDLTAKYGTLFVIAAGNSGGPGTVSSPSTAEAALSVGAVDRDDKLAYFS
                     SRGPRTGDSGLKPEVTAPGVEIVAAKAGSTEHVSASGTSMATPHVAGAAALLKQQHPEW
                     TPAQLKSALATTAKPAPGVSPFDQGAGRIDVAKAINQVLTADVVAVNLGLHEWPHGDDK
                     PETRSITYSNPTDADITLDVKITSGAPQGMFSLSASKVTVPAKGTAAVKVTGDTRVGTQ
                     DAAYTGEVVATSGTHTVRTLVGVEREVESYTLTIDMIGRDGQDPVDALTFVSGLDIPTS
                     KVVFGGGDQKIRLPKGTYVVDSSISSPNGMDMLFHPTVKVTGDLAIKQDARLAQPLSVT
                     VPEPTGDFVVGELSYRRAYGDRVLQAGLIAFGGFDGISVAQVGGPLSPKELASMATLHA
                     NSAAEANVYYRLAYPVAGSLPNGLTRAPGKKDLARVEATIGGLKPDSRALKGEFPTSVH
                     GVGGGSALYQVPAGSKAVDYVTTEDLRWQSIVSITHANGSREEAVSGFRTYEAGKTYQE
                     RENQPVFSPALPESPYPYAERNGTQLFIRVPMLSDASSGQGGSTVDSFRAALYRNGVLI
                     GEGAGGNFQNVPLPEGDFRAEASLDRSSQFEFSSKVSAAWTFRSKGTPGVTDTIPLSVV
                     RFAPKLDDNGAVPAGTLQRVGLVTQSQGDTGKVRITGVQYSHDDGKSWRNAGVAGNSAL
                     IYHPAGAKWTSLKATAKDARGNTVEVTVIHAYKVS"
     misc_feature    4437..4446
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    4534..4548
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.16"
                     /tool="antismash"
     gene            complement(4637..5008)
                     /locus_tag="C8D88_RS44745"
                     /old_locus_tag="C8D88_12540"
     CDS             complement(4637..5008)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671914.1"
                     /locus_tag="C8D88_RS44745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D88_12540"
                     /product="hypothetical protein"
                     /protein_id="WP_233440285.1"
                     /transl_table=11
                     /translation="MAEIIDRNDPSVARERLAAAQAELLNALLANGPAPAGFDEQRLDV
                     ERRALLSKRRGIVRMLGPAVADELGDRFRPLFDAYALENPRRAGSRARDDAAAFAEWCR
                     AAGELSAKRKARWKFWQSN"
     gene            complement(5001..5819)
                     /locus_tag="C8D88_RS44750"
                     /old_locus_tag="C8D88_12541"
     CDS             complement(5001..5819)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684640.1"
                     /locus_tag="C8D88_RS44750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D88_12541"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_109642603.1"
                     /sec_met_domain="DUF692 (E-value: 3.6e-86, bitscore: 280.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGLADLGVGIGWRSEIDLTVERLPGVDFVEVVAENLHIGHLPES
                     VTLLLERGVPVLPHAVSLSLGGADPVDLGRVEHLGKVAEAVGAPMVSDHVCFVRAGGLD
                     SGHLMPVPRTRDALDVLVANVELAQRNLPVPLALENVAALLEWPDGELSEGQFLAELVE
                     RTDCWLLVDVANLYANARNIGTDPERFLDEIPLERLAYVHVAGGVDHGDVYHDTHAHAV
                     LPEVLGVLDELCSRVKPPGVLLERDDNYPSDTEIASELAAIRAVVDKHRG"
     gene            complement(5819..6601)
                     /locus_tag="C8D88_RS44755"
                     /old_locus_tag="C8D88_12542"
     CDS             complement(5819..6601)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="C8D88_RS44755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D88_12542"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_170155375.1"
                     /transl_table=11
                     /translation="MTTQGTQLSAEQVGYLTAGPGRAAETALARLIDAGLVRVSRDGLV
                     SAVHQNNHGATTAIEARILSYARTPVRFDTIVRSAAHSTEMSMLHQHLLDRKLLQRPRR
                     RNEGWCFSLVIAVVLVLLGFVALEFLLGVPIAVLFFLRQRGRKAITGAGREALLRVTAT
                     DRVHAVAVYGFCGRVNRRPVADLFELSQSIVRMLPLKQNRKRRSSSDGGGSGSSCSSCG
                     SGCGSSSCSSSSGSSCSSSSSSSCGGSSCGGGGGGGGD"
     misc_feature    complement(6607..6621)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.17"
                     /tool="antismash"
     gene            complement(6625..7458)
                     /locus_tag="C8D88_RS44760"
                     /old_locus_tag="C8D88_12543"
     CDS             complement(6625..7458)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="C8D88_RS44760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D88_12543"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_146231921.1"
                     /transl_table=11
                     /translation="MAKSSTPQANWAAEEIGFLAGGPGRAAEAALARLMDGGLVRFSRE
                     GLVTAVHQNGHGATSPLEASILAGLSGGGRPIGLVVGPAAHSQEMGALHRNLVEKALAH
                     RQWGKTSGGRVALRVLLVLLGIAFLAAALFVDLNLLAVTLVAWFVAFLLRDKKMLTPIG
                     RGVLGHAQRNPRSRVDAVALHGLRGVVGRRRVADLYGLPPGILYPPPVHHHGKQKSHSD
                     SVGCGGVHEHHNCASSSSCSSSSCSSSSCSSSSSSCSSSSCGSSSSSSSCSSSSS"
     gene            complement(7551..8423)
                     /locus_tag="C8D88_RS44765"
                     /old_locus_tag="C8D88_12544"
     CDS             complement(7551..8423)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="C8D88_RS44765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D88_12544"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_109642609.1"
                     /transl_table=11
                     /translation="MQRPWGLSGPEFLQLYWIGLALAVLVAIIVRVRVRAGHSDQPVRS
                     LDMDELAYLAGGPKRVVETSIARLLTAGELRTSRRGTVQVTTTTSSLNPVDRAVITDSQ
                     RYTNRTVGLMIPSVSKDGVVIGIGRRLEEMGLVVHPDVAKSALRKGSIALWVLLAIGVA
                     RWVNGVAIDAPVGWLTLQLVITGALIFLLTRRGKHVRTSKGNRVLNEARTTSSGRAATS
                     DEALYAGAAGLVLFGGLAVYPDLAVRSSLLAASGGGYTGYTGCSAGSSSCSSGGGGGGC
                     GGGCGGGGS"
     gene            complement(8674..9096)
                     /gene="msrB"
                     /locus_tag="C8D88_RS44770"
                     /old_locus_tag="C8D88_12545"
     CDS             complement(8674..9096)
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422371.1"
                     /locus_tag="C8D88_RS44770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D88_12545"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_109642611.1"
                     /transl_table=11
                     /translation="MEPVVGATPKVVRSEEEWRAQLNPAEYAVLRQAGTERPWTGEYTE
                     TKTEGVYECRACGAELFRSDTKFDSHCGWPSFFSPLAGDAVLLREDRTLGMTRVEVLCA
                     TCHSHLGHVFEGEGYPTPTDQRYCINSVSLKLVPSE"
     misc_feature    complement(9095..9111)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 19.68"
                     /tool="antismash"
     gene            complement(9193..9483)
                     /locus_tag="C8D88_RS44775"
                     /old_locus_tag="C8D88_12546"
     CDS             complement(9193..9483)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099308.1"
                     /locus_tag="C8D88_RS44775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D88_12546"
                     /product="hypothetical protein"
                     /protein_id="WP_109642613.1"
                     /transl_table=11
                     /translation="MGDSKDENEKFQEELIGLDPQDPETQAFAEHLDRMKRQRPGYTVE
                     GYLEGVQDFADSANRTTGHQRLVAVIVVGMILLGVILTIWFALSDIITIIF"
     gene            complement(9508..10461)
                     /locus_tag="C8D88_RS44780"
                     /old_locus_tag="C8D88_12547"
     CDS             complement(9508..10461)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032563.1"
                     /locus_tag="C8D88_RS44780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8D88_12547"
                     /product="asparaginase"
                     /protein_id="WP_109642615.1"
                     /transl_table=11
                     /translation="MGHEVVAEVWRGDFLESVHHGTVVATDVAGIPALAVGRPGDVAFP
                     RSSNKPLQALAMLRAGLGLDGELLALACASHSGEDFHVEGALRILASAGLGEDALQCTP
                     DLPLGEEARARHLARGLAAAPKYMNCSGKHAAMLATCVANGWSTHDYLDPTHPLQVATR
                     ETIEDVAGEPVAQVGVDGCGAPIFAISLTGLARAFGRLASAPEGTLEHRVAVAMNAYPQ
                     WVGGTGRDVTVLMDALPGVIAKDGAEGVYAVGLPSGEGVALKIADGSSRARAVVVIAVL
                     KRLGVDVSKVEDLATVPVLGHGRAVGAVKPSLALAG"
     misc_feature    10497..10512
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.49"
                     /tool="antismash"
ORIGIN
        1 ctcggcggcg acgcccgagt acctggagtg cgacggcgtc gtgctcgcgg tcccggctcc
       61 gtcggcgcgc aaactgctgt ccgagatcgc gccgcaggtg tccgccgcgt acggcaaggt
      121 cgaggtcgcc tcgatggccg tcgtcgcgct ggcactgccg ccgggcaccg aactgccgga
      181 ccgctccggc atcctgatcg cggaggggga gcggtacagc gaccgcatca ccccgttcac
      241 cgccaaggcg ttcacgttct ccagccgcaa gtggtcgcac ttcggcgagc ccgtgctggt
      301 gcgcggctcg gtcgggcgcc gcggcgagac ggcgctcctg cagcgcagcg acgcggacct
      361 cgtgaaggcc gtgcgcaacg acctgtacga gctcaccggg atcaaggccg agcccatcga
      421 ccacgtggtg cagcgctggg gcggaggcct gccgcagtac ggcgtcgggc acgtcgacct
      481 cgtcgagacc atcgagaagg gcatcggcga cgtacccggc ctcgcggtgg ccggcgccac
      541 gctgcacggc gtcggcgtac cggcgtgcat cgccaccgcc gacgcggccg ccgcccgtgt
      601 cgcagcacac ctgctgggcc gcgttcgccg ttgacagggc cgtgccaaga tggacgtatg
      661 gcgcgcctga actacaacga gctcaacgac accatccgct acacgatgtg gtcggtgttc
      721 cgcgtcgagc cggggaagct gccggaagac cgcagcgccg ccgcctccga gacgcagaac
      781 tacctcgacg cgttggaagg caagggcgtc gtcgtccgcg gtgtctacga cgtggccggt
      841 ctgcgtgccg acgcggactt catgatctgg tggcacgccg aggagatcga gcaggtgcag
      901 gccgcctaca cgggcttccg ccgcaccccg ctcggcaagg tctccacgcc ggtctggtcg
      961 caggtcgcgc tgcaccgccc cgccgagttc aaccgcagcc acatccccgc gttcctcgcc
     1021 aacgaggagc cgcgcaagta cgtctgcgtc tacccgttcg tgcgctccta cgagtggtac
     1081 ctgctgccgg aggaggaccg ccgcaagatg ctcgcggacc acggcaagga agcccgcgac
     1141 ttccctgacg tgcgcgcgaa caccgtcgcg tcgttcgcgc tgggcgacta cgagtggatg
     1201 ctcgctttcg aggccgacga gctgcaccgc atcgtcgacc tgatgcggca cctgcgcggc
     1261 accgaggcgc ggctgcacgt gcgtgaggag atccccttct acacgggcac ccgcgtcccg
     1321 gccgccgagc tggtcacgaa cctgccgtga cgagaaaggg ccgcccctga accgggcggc
     1381 cctctccgtt cagcgcacta gctcaccttg taggcgtgga tcacggtcac ctcgaccgtg
     1441 ttgcccctgg cgtccttcgc cgtggccttc agcgaggtcc acttggcgcc ggccgggtgg
     1501 tagatcaacg cgctgttgcc ggccacaccc gcgttgcgcc acgacttgcc gtcgtcgtgc
     1561 gagtactgga cgccggtgat gcgcaccttg cccgtgtcgc cctgggactg ggtgaccaga
     1621 ccgactcgct gcagcgtgcc cgcgggcacc gcgccgttgt cgtcgagctt cggcgcgaac
     1681 cgcacgaccg agagcgggat ggtgtcggtc acgcccggcg tgcccttgga cctgaacgtc
     1741 cacgccgcgg acaccttgct gctgaactcg aactgcgagc tccggtcgag gctcgcctcc
     1801 gcgcggaagt ctccctccgg gagcggcacg ttctggaagt tgcccccggc gccttcaccg
     1861 atcagcacac cgttgcggta cagcgccgcc cggaaactgt ccacagtgga gccaccctgg
     1921 ccgctgctcg cgtcgctcag catcggcacg cggatgaaca gctgggtgcc gttgcgctcg
     1981 gcgtacgggt acggggactc gggcagcgcc ggcgagaaca ccggctggtt ctcccgctcc
     2041 tggtaggtct tgcccgcttc gtacgtgcgg aacccggaga cggcctcctc ccggctgccg
     2101 ttcgcgtggg tgatgctcac gatcgactgc cagcgcaggt cctcggtcgt cacgtagtcg
     2161 accgccttgc tgcccgccgg cacctggtag agcgccgaac cgccgcccac gccgtgcacc
     2221 gacgtcggga actcgccctt gagcgcccgg ctgtccggtt tgagaccgcc gatggtggcc
     2281 tccacgcgtg ccaggtcctt cttgcccggc gcgcgcgtca ggccgttggg caggctgccg
     2341 gcgaccgggt aggccagccg gtagtacacg ttggcctcgg ccgcggagtt cgcgtgcagc
     2401 gtcgccatcg acgccagctc cttcggcgag agcgggccgc cgacctgcgc caccgagatg
     2461 ccgtcgaagc caccgaacgc gatcagccct gcctgcagga ccctgtcgcc gtacgcgcgc
     2521 cggtacgaga gctcaccgac gacgaagtcc ccggtgggtt ccggcaccgt caccgacagc
     2581 ggctgcgcga ggcgggcgtc ctgcttgata gccaggtcgc cggtcacctt caccgtcgga
     2641 tggaacagca tgtccatgcc gttcggcgag ctgatcgagg agtcgaccac gtacgtgccc
     2701 ttgggcaggc ggatcttctg gtcgccacca ccgaagacca ccttggacgt cgggatgtcc
     2761 aggccgctga cgaacgtcag cgcgtccacg gggtcctgcc cgtcgcggcc gatcatgtcg
     2821 atcgtgaggg tgtagctctc cacctcgcgc tcgacaccga cgagcgtgcg gacggtgtgc
     2881 gtgccggacg tggcgacgac ctcgccggtg tacgcggcgt cctgcgtgcc gacgcgggtg
     2941 tcgccggtga ccttgacggc cgccgtgccc ttggcgggaa cggtgacctt cgaggcgctc
     3001 agcgagaaca tgccctgcgg cgcaccgctg gtgatcttca cgtcgagcgt gatgtcggcg
     3061 tcggtcgggt tgctgtaggt gatcgacctg gtctccggct tgtcgtcacc gtgcggccac
     3121 tcgtgcaggc cgaggttgac cgcgacgacg tccgcggtca gcacctggtt gatcgccttg
     3181 gccacgtcga tgcgaccggc gccctggtcg aacgggctca cgcccggtgc cggcttggcc
     3241 gtggtggcca gcgccgactt gagctgcgcg ggcgtccact ccgggtgctg ctgcttcagc
     3301 aacgccgcgg caccggccac gtgcggagtc gccatggacg tgccgctcgc cgagacgtgc
     3361 tcggtggagc ccgccttggc ggcgacgatc tcgacgcccg gtgcggtcac ctcgggcttg
     3421 agcccgctgt caccggtgcg agggcccctg ctggagaagt aggcgagctt gtcgtcgcgg
     3481 tcgacggcgc cgaccgacag cgcggcctcg gccgtgctgg gtgagctcac cgtgcccggc
     3541 ccgcccgagt tgcccgccgc gatcacgaac aacgtgccgt acttggcggt gaggtcgttg
     3601 accgcctgct ccagcgggtc gatctccggg gtgtcgcctc ccccgaggct catgttgacg
     3661 acctgggcgc cctgctcggc ggcccagcgc atgccgtcga ggatccacga ctcctggcag
     3721 ccgaactcga cgcagacctt ggcgtcgagt agctgggcgc ccggtgcgac gccgccgtac
     3781 tgcgacccgg cgctcgcgac ggtcgcgccg acgtgcgtgc cgtggccgac cctgtcggtg
     3841 gtgtccgggt cgctggtgaa gttcttctcc gcgatctgct gacccttgag gtccgggtgg
     3901 ttctcgtcca cgccggtgtc gacgatcgcg accttgacgc ccttgccggt gatgccgcgc
     3961 tggtgcgcgg ccggcgcgcc gatctgcgcg acgctgcggt cgaggctcgg cttgcggatg
     4021 ccgtcgagcc agatcttgtc accggaggcg gcctccgccc gcagagcggc ccagtcggcc
     4081 gaggaccgcg ctccgagtct cgcctgaccg tccacgatca acggcatccg gtcccggtag
     4141 ccgaactcca gcagcgtggt gacgtcgaag agtctgcggt cgagccggcc cgccgccagg
     4201 ggcccgaacg cgtccgtcgg cacgaccatc gtgtgcccgt cggcaccgcg gtgggtcttg
     4261 aaggtcatcc ccttgcggcc ctcgccgggc tgaatcgcga cgacctgctg tgcctgtccc
     4321 agaaggacct tgtctccggt gacgagggtg acctcgtcga ccgcccgttg tggaccggtg
     4381 ggttgcggtg cggccgacgc tgcggtggcc gttcccgcgg tcagtgcgag cgccgcgaag
     4441 gcggccccca gcactttcac atttccgcgc attcgaatcc cctcatggtt cgaaatgctg
     4501 cgcgctgtgg tccgcgcagg ccggaaggcc ttctgcgcaa tgtgaaaatg gccccccgac
     4561 cctttcgaac gtatcgacgg tggcgggggt cacacagggc tgaacgggcg attcttgagg
     4621 gagttctgtt cgcccgtcag ttcgactgcc agaacttcca gcgcgccttg cgtttggctg
     4681 acagttctcc tgcggcacgg caccattcgg cgaacgccgc cgcgtcgtcg cgggcacgcg
     4741 agcccgcgcg acgagggttt tccagggcgt acgcgtcgaa gagcggccgg aaccggtcgc
     4801 cgagctcgtc ggcgacagcg ggccccagca tccgcacgat gccgcggcgt ttggacagca
     4861 acgcgcggcg ttcgacgtcc agtcgttgtt cgtcgaatcc cgccggagcc ggtccgttcg
     4921 cgagcaatgc atttagcagc tcggcctgtg cggcggcaag cctttcgcgt gcgaccgaag
     4981 gatcattccg gtcgatgatt tcagccacgg tgtttgtcca cgacggcgcg gatcgcggcc
     5041 agttcggacg cgatttcggt gtcggacggg tagttgtcgt cgcgttccag cagaacgccg
     5101 ggtggtttca cgcgcgagca cagttcgtcg aggacgccga gcacttccgg cagcaccgcg
     5161 tgggcgtggg tgtcgtggta gacgtcgccg tggtcgacgc cgccggccac gtgcacgtaa
     5221 gccaggcgct ccagcggaat ctcgtcgagg aaccgctccg ggtccgtgcc gatgttgcgc
     5281 gcgttcgcgt acaggttcgc gacgtcgacg agcagccagc agtccgtccg ttcgacgagc
     5341 tcggcgagga actggccctc ggacagctcg ccgtccggcc actccagcag cgcggcgacg
     5401 ttctccagcg cgagcggcac cggcaggttc cgctgcgcca gctcgacgtt cgcgacgagc
     5461 acgtcgagcg cgtccctggt gcgcggcacg ggcatcaggt ggccggagtc gagcccgccc
     5521 gcgcggacga agcagacgtg gtcgctcacc atcggcgcgc cgacggcttc cgcgaccttg
     5581 ccgaggtgct cgacgcgacc gaggtcgacc gggtccgcgc caccgagcga cagcgacacg
     5641 gcgtggggga gcaccggaac tccgcgctcc agcagcagcg tcaccgactc cggcaggtgt
     5701 ccgatgtgga ggttctccgc gaccacctcg acgaagtcca cgccgggcag ccgttccacg
     5761 gtcaggtcga tctccgaacg ccagccgatt ccgacgccca ggtccgcgag gcccgccatc
     5821 agtcgcctcc tcctccgcca ccgccgccgc agctgctgcc accacagctg ctgctgctcg
     5881 acgacgagca gctgctgccg ctgctcgatg aacaactact gctaccacac ccgctgccgc
     5941 agctgctgca actcgacccg ctgccgccac cgtccgaaga ggaccgccgc ttgcggttct
     6001 gcttcagcgg cagcatcctg acgatgctct gggagagctc gaagagatcg gcgaccgggc
     6061 ggcggttcac cctgccgcag aagccgtaga ccgccacggc gtgcacccgg tcggtcgcgg
     6121 tcacccgcag gagggcctcc ctgcccgccc cggtgatggc cttgcgcccg cgctgccgca
     6181 ggaagaacag cacggcgatc gggacgccga gcaggaactc gagcgccacg aagccgagca
     6241 ggaccagcac gaccgcgatc acgagcgaga agcaccagcc ctcgttgcgg cgacgcgggc
     6301 gttgcaggag cttccggtcg agcaggtgct ggtgcagcat cgacatctcg gtgctgtgcg
     6361 cggccgaccg cacgatcgtg tcaaaccgga cgggtgtgcg cgcgtagctc aggattcgcg
     6421 cctcgatcgc cgtggtcgcg ccgtggttgt tctggtgcac ggccgagacg aggccgtcgc
     6481 gcgacacccg aacgagaccg gcgtcgatca gccgggcgag cgcggtctcg gcggcacgcc
     6541 cgggacccgc cgtcaagtaa ccgacctgtt ccgcggaaag ctgcgtcccc tgtgttgtca
     6601 ccggtgtgac catgagtttc cccctcaact gctggaactg ctgcagctgc tgctactact
     6661 actgctaccg caactcgaac tgctgcaact ggaactgctg ctcgaacagc tgctgctgct
     6721 gcaactgctg ctggagcagg aagacgacga cgcgcagttg tggtgctcgt gaacgccgcc
     6781 gcacccgaca ctgtccgaat gcgacttctg ctttccgtgg tggtgcaccg gtggcgggta
     6841 caggattccg ggcggcaagc cgtacaggtc ggcgaccctc cggcggccga ccacaccacg
     6901 cagtccgtgc aacgccacgg catccacccg gctgcgcggg tttcgctgtg cgtgccccag
     6961 gacgcccctg ccgatcggcg tgagcatctt cttgtcgcgc aggaggaacg ccacgaacca
     7021 cgccacgagc gtgacggcca ggaggttcag gtcgacgaag agcgccgccg cgaggaacgc
     7081 gatgccgagc aggaccagca ggacccgcaa cgccaccctg ccgccgctcg tcttgcccca
     7141 ctggcggtgg gccagcgcct tctcgaccag attgcggtgc agtgcaccca tctcctggct
     7201 gtgcgccgcc ggccccacca ccaggccgat cgggcgaccc ccgccggaca gcccggcgag
     7261 gatggacgcc tcgagcgggc tcgtcgcgcc gtgaccgttc tggtggacgg cggtcacgag
     7321 tccctcacgg gagaaccgca cgagaccgcc gtccatcagc cgggcgagag cggcctccgc
     7381 cgccctgccc ggtccccccg ccaggaaccc gatctcctcc gcggcccagt tcgcctgcgg
     7441 tgtcgaagac ttggccatca gcactcccct cactggtgcc cgtgcatctt cgacgtgaga
     7501 tctaccccca ggttcctggt gttacctgat tcagaccgga cagcgggaaa tcagctgccg
     7561 cccccgccgc agccgccccc gcagccgcca cctccgccac cgctcgaaca cgatgaagaa
     7621 cccgccgaac acccggtgta gccggtgtag ccgcccccgg aagcggcgag caacgagctg
     7681 cgcaccgcca gatcggggta caccgccaga ccgccgaaca gaaccagccc ggccgcgccc
     7741 gcgtacaggg cctcgtcgga cgtggcggca cggccgctgc tcgtggtgcg tgcctcgttc
     7801 agcacgcggt tccccttgga cgtgcggacg tgcttgccgc gccgcgtgag gaggaagatg
     7861 agcgcaccgg tgatgacgag ctgcaacgtc agccagccga cgggcgcgtc gatcgcgacg
     7921 ccgttgaccc agcgggccac accgatcgcg agcagcaccc acagcgcgat ggatcccttg
     7981 cgcagcgcgc tcttcgcgac gtccgggtgg accaccaggc ccatttcctc gagcctgcgg
     8041 ccgatcccga tgaccacgcc gtccttggag acggagggga tcatcagacc gacggtgcgg
     8101 ttggtgtagc gctggctgtc ggtgatgacg gcgcggtcca ccgggttcaa ggagctcgtg
     8161 gtggtcgtga cctgcaccgt gccacgccgt gaggtgcgaa gctcacccgc ggtgagcagc
     8221 cgcgcgatgg acgtttcgac cacgcgcttc ggaccgcccg cgagataggc gagctcgtcc
     8281 atgtcgaggg agcgcacggg ctggtcgctg tgacccgccc gcacgcgtac ccgaacgatg
     8341 atcgcgacca gcaccgccag tgccagcccg atccagtaga gctgaaggaa ctcaggtccg
     8401 gacaaccccc aaggcctctg catgcgaccc accccctgac tgccccgaaa gtgcttcggt
     8461 ggcgtccatg gtgtggtccg ggccacagcc ccgcaatgct agttgactaa gccttagggt
     8521 tgtccgtctt ttgtcaccag ttcgtcgctc tgaccagctc tcagctggac gaaccgccgt
     8581 cgactccgct ggagccttca ccgtcggtac cgaagaagat cattgcgcct cccaggtcgg
     8641 cccggcccga tcgaccggac tcggctcagt ctcctattcg gacggaacga gtttcaagga
     8701 gacggagttg atgcagtacc gctgatccgt cggcgtggga tacccctcac cttcgaaaac
     8761 atgcccgagg tggctgtggc aggtggcgca gagcacttcg acgcgggtca tgccgagggt
     8821 gcggtcctcg cgcaggagca ccgcgtctcc cgccagcggc gagaagaacg acggccagcc
     8881 gcagtgcgag tcgaacttgg tgtcgctgcg gaacagctcg gctccgcacg cacggcactc
     8941 gtagacgccc tcggtcttgg tctcggtgta ctcgccggtc cacgggcgct cggtgcctgc
     9001 ctggcgcagc accgcgtact cggcggggtt cagctgggcg cgccattcct cttcggaacg
     9061 cacgactttg ggggtggcgc cgacaacagg ttccatgtcc ctttataacg cgggcacggt
     9121 cctcgggctt cccggcgacc gccgcgtgca cccctgccgt aagaccttgt tgagaagtcg
     9181 tcttcgtttg gctcagaaga tgatggtgat gatgtcgctc aacgcgaacc agatcgtcag
     9241 gatgacgccg agcaggatca tcccgacgac gatgacggcc accagccgct ggtgcccggt
     9301 cgtgcggttc gccgagtcgg cgaagtcctg cacgccctcc aggtacccct cgacggtgta
     9361 gccgggtcgc tgccgcttca tccggtcgag gtgctcggcg aacgcctgcg tctcggggtc
     9421 ttgggggtcg aggccgatca gctcctcctg gaacttctcg ttctcgtcct tcgaatcgcc
     9481 cataacgctc aagggtagtg ccaggaatca accggccagg gcaagtgagg gcttgacggc
     9541 tccgacggcg cgtccgtggc cgaggacggg caccgtggcg aggtcctcga ccttgctcac
     9601 gtccaccccg agccgcttga gcaccgcgat cacgacgacc gcgcgggcgc gcgacgagcc
     9661 gtcggcgatc ttcagggcga caccctcgcc gctcggcagg ccaacggcgt acacaccctc
     9721 ggcgccgtcc ttcgcgatga cgccgggcaa ggcgtccatc agcaccgtga cgtcacggcc
     9781 ggtgccgccg acccactgcg ggtaggcgtt catcgcgacg gcgacgcggt gttccagggt
     9841 gccttccggg gcagaagcca ggcggccgaa cgcgcgcgcc aggccggtga ggctgatggc
     9901 gaagatcggg gcaccgcagc cgtcgacgcc gacctgggcg acgggctcgc cggccacgtc
     9961 ttcgatcgtc tcgcgcgtgg cgacctgcag cgggtgcgtc ggatccaggt agtcgtgcgt
    10021 cgaccagccg ttggcgacgc acgtggcgag catggcggcg tgcttgcccg agcagttcat
    10081 gtacttcggg gcggcggcga gaccgcgggc gaggtgccgg gcgcgggcct cctcgccgag
    10141 cgggaggtcg ggggtgcact gcaacgcgtc ctcgcccagg ccggccgagg ccaggatccg
    10201 cagcgcgccc tcaacgtgga agtcctcgcc cgagtgcgag gcgcaggcca gggcgagcag
    10261 ctcgccgtcg aggccgaggc cggcgcggag catcgcgagg gcctggagcg gcttgttcga
    10321 cgagcgcggg aacgcgacgt cgccgggacg tccgacagcc agcgcgggga tgccggcgac
    10381 gtcggtggcg acgacggtgc cgtggtgcac ggactccagg aagtcgcccc gccagacctc
    10441 tgccacaacc tcgtgtccca tgtcgcccct cccgtgtgcc cgagaaactg ccaactgtcg
    10501 acagttgaca acgcggctaa tgtaaccagg agacggcctg tgccgtccag agcaggagac
    10561 gaacgtgaca cctccgctgc acctcagtct ggccgggcag gccgtggacg tcctgcgcga
    10621 gcaggtgctc accggggaga tcccgccggg ctcacgggtc aacgaggtcg aggtggcgca
    10681 gaagctcggg atcagccgcg ggccgttgcg cgaggcgatc cggcacctgg cgtccgaagg
    10741 gctgctcacg ctggtgccgc accggggcgc gttcgtgccc gcggcggacg ccgacgaggt
    10801 caaggcgttg ttcgagctg
//
