LOCUS       NZ_CP029361            10399 bp    DNA     linear   CON 31-MAY-2024
DEFINITION  Streptomyces globisporus strain TFH56 chromosome, complete genome.
ACCESSION   NZ_CP029361
VERSION     NZ_CP029361.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces globisporus
  ORGANISM  Streptomyces globisporus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7488586)
  AUTHORS   Cho,G.
  TITLE     Complete genome sequence of Streptomyces globisporus TFH56 isolated
            from tomato flower
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7488586)
  AUTHORS   Cho,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2018) Division of Applied Life Science, Gyeongsang
            National University, Jinjudaero, Jinju 52828, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-MAR-2018
            Assembly Method :: canu v. 1.7; arrow polish v. SMRT link 5.1.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 37.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003147545.1-RS_2024_05_31
            Annotation Date                   :: 05/31/2024 02:17:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,917
            CDSs (total)                      :: 6,829
            Genes (coding)                    :: 6,492
            CDSs (with protein)               :: 6,492
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 337
            CDSs (without protein)            :: 337
            Pseudo Genes (ambiguous residues) :: 0 of 337
            Pseudo Genes (frameshifted)       :: 240 of 337
            Pseudo Genes (incomplete)         :: 121 of 337
            Pseudo Genes (internal stop)      :: 7 of 337
            Pseudo Genes (multiple problems)  :: 31 of 337
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:15:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6040033
            Orig. end                         :: 6050432
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029361.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="23"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    7..20
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.97"
                     /tool="antismash"
     gene            56..1114
                     /locus_tag="DIJ69_RS27165"
                     /old_locus_tag="DIJ69_27170"
     CDS             56..1114
                     /NRPS_PKS="Domain: Aminotran_5 (19-347). E-value: 9.5e-25.
                     Score: 79.2. Matches aSDomain:
                     nrpspksdomains_DIJ69_RS27165_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070339.1"
                     /locus_tag="DIJ69_RS27165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_27170"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_044371087.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.3e-17, bitscore:
                     62.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTILAPIHAAAVEFAPEVTYLNTSSWGLLPRRTIAAVKALADENA
                     AGRRVGAGSFEAVEAARAGFARLVGVHPDRVATGSSVTVHVGLIAASLPPGAEVLCPEG
                     EFSSVVSPFALRGDLRMRYAPLADLAGAVRDSTALVAFSAVQSADGRVADLAAVRAAAA
                     AHGARTLLDATQAAGWFPVDAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQDTLPALFA
                     GWVAAGAPWASNYGPLERLAPTARGFDEPPAFLSYHGAEHSLALLAEVGADALYAHATG
                     LAARLREGLATLGHKAVPGDSAIVAVPGLDGWQPELVEAGIAVSAPAGNLRISCHLYNT
                     EADVDRLLDFLA"
     aSDomain        113..1096
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DIJ69_RS27165_Aminotran_5.1"
                     /evalue="9.50E-25"
                     /label="DIJ69_RS27165_Aminotran_5.1"
                     /locus_tag="DIJ69_RS27165"
                     /protein_end="347"
                     /protein_start="19"
                     /score="79.2"
                     /tool="antismash"
                     /translation="YLNTSSWGLLPRRTIAAVKALADENAAGRRVGAGSFEAVEAARAG
                     FARLVGVHPDRVATGSSVTVHVGLIAASLPPGAEVLCPEGEFSSVVSPFALRGDLRMRY
                     APLADLAGAVRDSTALVAFSAVQSADGRVADLAAVRAAAAAHGARTLLDATQAAGWFPV
                     DAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQDTLPALFAGWVAAGAPWASNYGPLERL
                     APTARGFDEPPAFLSYHGAEHSLALLAEVGADALYAHATGLAARLREGLATLGHKAVPG
                     DSAIVAVPGLDGWQPELVEAGIAVSAPAGNLRISCHLYNTEADVDRL"
     gene            complement(1171..1686)
                     /locus_tag="DIJ69_RS27170"
                     /old_locus_tag="DIJ69_27175"
     CDS             complement(1171..1686)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123628.1"
                     /locus_tag="DIJ69_RS27170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_27175"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_044371089.1"
                     /transl_table=11
                     /translation="MTRATPAEDACTNALPTAALGGPVSHAVSRVARLHRIAAGKVLKG
                     VDLYPGQELLMMHLWDRGAVRQTELIRALELDPSTVTKMLQRLEQTGHVRRRPDPADRR
                     AVLVEATDESCALHTAVENAWGSLEEHTLAGLDPAERTELVRLLAKVEENLCRETEDCP
                     DRRGAASG"
     gene            1883..2953
                     /locus_tag="DIJ69_RS27175"
                     /old_locus_tag="DIJ69_27180"
     CDS             1883..2953
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="DIJ69_RS27175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_27180"
                     /product="alkene reductase"
                     /protein_id="WP_044371091.1"
                     /transl_table=11
                     /translation="MTTVFDPIDLSGTPLANRIALAPMTRSRAGEGGTATELTATYYAQ
                     RASAGLVITEGIQPSVVGQGYPSTPGLHSAEQVASWRTVTDAVHARGGRIFAQIMHAGR
                     IGHPVLLPDGLTPVSASPVKAEGQVYTHVGPKDFVEPHELTDAEVRATVADFVTASRNA
                     VEAGFDGVELHGANGYLIHQFLAPNTNLRTDAWGGSDEARTRFAVEVVEAVAAEIGPER
                     TALRISPGNPYNDIDEPAPEATYTALVRAIEPLGLAYLHILEATPIRELTLALRKAFSG
                     TLVINVHSEGPTGPGDHTVIDDGIADLISYGALFLANPDLPARLKAGGPFNTPDPSTFF
                     GGDAKGYTDYPALDAV"
     misc_feature    1884..1898
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.18"
                     /tool="antismash"
     gene            complement(2929..3963)
                     /locus_tag="DIJ69_RS27180"
                     /old_locus_tag="DIJ69_27185"
     CDS             complement(2929..3963)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070337.1"
                     /locus_tag="DIJ69_RS27180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_27185"
                     /product="VOC family protein"
                     /protein_id="WP_044371093.1"
                     /transl_table=11
                     /translation="MTSHVRHITIDCADAYALGSFWAQVLGAPLSDEDLPGDPEALLET
                     PGAAVLFVQVAEKKQSKNRVHLDIQPHDRSRDEEVERLLALGATLVGDHRRPNGRGWAT
                     LADPEGNEFCVECSAAERAALSGTRLPVTADDVTTAVRLAVDLLATAPADRWEARAGSL
                     EWTCWETAEHLGDDLFAYAVQLGPRTPPLDREVPFRWAPDREGGPHNAVFADRAAGPAG
                     LLGTLEASGALLASMTRTTPPGVRSYHGYGISDPEGFAAMGVVETLVHTHDLAEGLGLE
                     WAPPAALCDRALARLFPDAPAGGDRWAVLLWATGRAELPGHPRRTAWRWDGRPLEDQTA
                     SSAG"
     gene            complement(4101..4994)
                     /gene="thpD"
                     /locus_tag="DIJ69_RS27185"
                     /old_locus_tag="DIJ69_27190"
     CDS             complement(4101..4994)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="DIJ69_RS27185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_27190"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_030813807.1"
                     /sec_met_domain="PhyH (E-value: 1.1e-36, bitscore: 125.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPIAAKDLQSF
                     EHDGFLSIGELIAPDEVAVYQAELDRLVSDPLIRADERSIVEKQSQNVRSVFEVHKISE
                     VFAALVRDERVVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENFDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIKLFTGKAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENAAQEPFAAP
                     IRRPEFIGARDFTPVK"
     gene            complement(5001..5399)
                     /locus_tag="DIJ69_RS27190"
                     /old_locus_tag="DIJ69_27195"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="DIJ69_RS27190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_27195"
                     /product="ectoine synthase"
                     /protein_id="WP_030813810.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.2e-53, bitscore:
                     178.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGEKHWITPGTMYLLDGHERHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEA"
     gene            complement(5491..6753)
                     /gene="ectB"
                     /locus_tag="DIJ69_RS27195"
                     /old_locus_tag="DIJ69_27200"
     CDS             complement(5491..6753)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 1.7e-72.
                     Score: 236.2. Matches aSDomain:
                     nrpspksdomains_DIJ69_RS27195_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="DIJ69_RS27195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_27200"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_030813814.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.6e-77, bitscore:
                     257.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQELCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGITPDIVTLSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALQAYWADGQMEKQTLARGEQIEQTLL
                     AICAEESTASFRGRGLVWGMEFEDPARASAVCARAFELGLLLETSGPQSEVVKLLPPLT
                     ITPEELEEGLRTLARCVRETA"
     aSDomain        complement(5665..6669)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DIJ69_RS27195_Aminotran_3.1"
                     /evalue="1.70E-72"
                     /label="DIJ69_RS27195_Aminotran_3.1"
                     /locus_tag="DIJ69_RS27195"
                     /protein_end="363"
                     /protein_start="28"
                     /score="236.2"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQELCHRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGITPDIVTLSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALQAYWADGQMEKQTLARGEQIEQTLLAICAEESTASFRGRGLVWGMEFE"
     gene            complement(6949..7479)
                     /gene="ectA"
                     /locus_tag="DIJ69_RS27200"
                     /old_locus_tag="DIJ69_27205"
     CDS             complement(6949..7479)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123622.1"
                     /locus_tag="DIJ69_RS27200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_27205"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_073776390.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLSIDAPRVEDGAAIWRIARDSQVLDLNSSY
                     SYLLWCRDFAATSVVARGEDGEPIAFVTGYIRPDRPQTLVVWQVAVDSAHRGKGLAATL
                     LDALTARVAADQGLSSVETTITPDNAASDRLFTSYAQRHDVALEQEVLFDGDLFPEETH
                     LPEVLYRIGPFPT"
     misc_feature    complement(7479..7493)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.36"
                     /tool="antismash"
     misc_feature    complement(7634..7649)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7644..7659
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    7719..7735
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: strong, score: 27.08"
                     /tool="antismash"
     gene            complement(7790..8896)
                     /locus_tag="DIJ69_RS27205"
                     /old_locus_tag="DIJ69_27210"
     CDS             complement(7790..8896)
                     /NRPS_PKS="Domain: Aminotran_5 (18-284). E-value: 1.6e-28.
                     Score: 91.6. Matches aSDomain:
                     nrpspksdomains_DIJ69_RS27205_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123620.1"
                     /locus_tag="DIJ69_RS27205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_27210"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_030813820.1"
                     /sec_met_domain="Aminotran_5 (E-value: 4.3e-22, bitscore:
                     77.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLTAGHFAAIERRVARLLATEQDVVITQGEALLPLE
                     GCIRSGARPGSTALNVVTGPYGQTFGNWLRDCGAQVVDLAVPFHTAVTAEQVAEALAEH
                     PETDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSARAWERIAANPAAPRRSYLSLLDWKERWIDGGRAALPHAP
                     AQLEMLGLEACVARIEDEGLETVMARHASAAAATRAGAVALGGGLEPYVHAARDAAPVA
                     TTLRAPAGTDAAALVAAALAADPALPLVAGGGALSKEMIRVNHYGADATRGAVLSSLAA
                     LGAALTDAGRQVDIEAARRAVSETWPSR"
     aSDomain        complement(8045..8842)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DIJ69_RS27205_Aminotran_5.1"
                     /evalue="1.60E-28"
                     /label="DIJ69_RS27205_Aminotran_5.1"
                     /locus_tag="DIJ69_RS27205"
                     /protein_end="284"
                     /protein_start="18"
                     /score="91.6"
                     /tool="antismash"
                     /translation="IERRVARLLATEQDVVITQGEALLPLEGCIRSGARPGSTALNVVT
                     GPYGQTFGNWLRDCGAQVVDLAVPFHTAVTAEQVAEALAEHPETDFVSLVHAEAATGNT
                     NPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAVSV
                     SARAWERIAANPAAPRRSYLSLLDWKERWIDGGRAALPHAPAQLEMLGLEACVARIEDE
                     GLETVMARHASAAAATRAGAVALGGGLEPYVHAARDAAPVATTL"
     gene            9057..10133
                     /locus_tag="DIJ69_RS27210"
                     /old_locus_tag="DIJ69_27215"
     CDS             9057..10133
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512211.1"
                     /locus_tag="DIJ69_RS27210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_27215"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_044371097.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 9.6e-17, bitscore:
                     59.8, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRVKGRVLVGPDEVRDELWAVDGRITYERPPGADDAVTVRGWAL
                     PGLVDAHCHVGLDRHGPVDAATAEKQALTDREAGTLLIRDAGSPSDTRWIDDREDLPKI
                     IRAGRHIARTRRYIRNYGHEIEPGDLVAYVAQEARRGDGWVKLVGDWIDRDAGDLTACW
                     PRGEVEAAIAEAHRLGARVTAHCFAEESLRDLVEAGIDCIEHATGLTEDTIPLFAERGV
                     AIVPTLVNIATFPDLAAGGEAKFPRWSAHMRQLYDRRYDTVRSAHDAGIPVYVGTDAGG
                     SLAHGLVAGEVAELVKAGIPPLEALSATAWGARRWLGRPGLDEGAPADLVVYDADPRAD
                     VRVLAAPRHIVLNGRVIG"
ORIGIN
        1 ttccccttaa ctctccgtga ttgacttcgg gttgaggggg ctccagggtg gacgcatgac
       61 gatcctcgcg cccatccacg ccgcagccgt cgaattcgcg cccgaggtca cctacctcaa
      121 cacctccagc tggggcctgc tgccccgccg gacgatcgcc gccgtcaagg ccctggccga
      181 cgagaacgcc gcgggccgcc gggtgggggc cggcagcttc gaggccgtgg aggccgcccg
      241 ggccggcttc gcccggctcg tcggggtcca cccggaccgg gtcgccaccg gcagctccgt
      301 caccgtccat gtcggcctga tcgccgcatc gctgccgccc ggcgccgaag tcctctgccc
      361 cgagggcgag ttctcctccg tggtcagtcc gttcgcgctc cgcggcgatc tgcgcatgcg
      421 gtacgcgccg ctcgcggacc tcgccggggc cgtccgggac tcgaccgcgc tggtcgcctt
      481 ctccgccgtg cagtcggccg acggccgggt cgccgacctc gccgccgtcc gtgccgccgc
      541 cgcggcgcac ggggcccgca ccctgctgga cgccacccag gcggcgggct ggttcccggt
      601 ggacgccggg gcgtacgact acaccgtcac cggcggtttc aaattcctgc tctgcccgcg
      661 cggtacgtcg ttcctgaccg tcaccgagga ggcgcaggac accctgccgg ccctcttcgc
      721 gggctgggtc gccgcgggcg ccccctgggc gagcaactac ggcccgctgg agcgcctcgc
      781 ccccaccgcc cggggcttcg acgagccgcc cgccttcctc tcgtaccacg gggccgagca
      841 ctccctcgcc ctgctcgcgg aggtcggcgc cgacgcgctg tacgcgcacg ccaccgggct
      901 cgccgcccgg ctgcgcgagg ggctcgcgac cctcgggcac aaggccgtgc ccggggattc
      961 ggcgatcgtc gccgtaccgg ggctcgacgg ctggcagccc gaactggtgg aggccgggat
     1021 cgccgtctcg gcgccggccg ggaatctgcg gatctcctgc cacctctaca acaccgaggc
     1081 cgatgtggac cggctgctgg acttcctggc ctgagccgta cggacgcggc tcggtccgta
     1141 cgggaacggc tcaggctgta cgggtacggc tcagccggac gcggccccgc gccggtcggg
     1201 gcagtcctcg gtctcccggc agaggttctc ctcgaccttc gccagcagcc ggaccagctc
     1261 cgtgcgctcg gccgggtcca ggcccgcgag cgtgtgctcc tccaggctgc cccaggcgtt
     1321 ctcgaccgcg gtgtgcagcg cgcagctctc gtccgtcgcc tcgaccagca cagcccgccg
     1381 gtcggccggg tcggggcggc ggcgcacatg cccggtctgc tccagccgct ggagcatctt
     1441 cgtgaccgtg gacgggtcca gctccagggc ccggatcagc tccgtctgcc ggaccgcccc
     1501 ccggtcccac aggtgcatca tcagcaactc ctgccccggg tagaggtcca cccccttgag
     1561 caccttgccc gccgcgatcc ggtgcagccg cgccacccgg gagacggcgt ggctgacggg
     1621 cccgccgagc gccgccgtgg gcagcgcgtt cgtacaggcg tcttccgcgg gggtggcgcg
     1681 ggtcatggcg tgctcctggg atcctcgggg cggtgagcgg cggcttcgtg ggggcctggg
     1741 tggcgccttc cgatgatgcc cgacggtttt cgacaacttt accttggtcg gccaatgaat
     1801 gggttacagt tgccctcggc ggcaatcatt ggccgaccac atactttctg ttggccgacc
     1861 acatacctat tcgggagccc tcatgaccac cgttttcgac ccgatcgatc tgtccggcac
     1921 ccccctggcc aaccgcatcg cgctggcccc gatgactcgc agccgggccg gcgagggcgg
     1981 caccgccacc gagctgacgg ccacgtacta cgcccagcgc gcctcggcgg gactcgtgat
     2041 cacggagggc atccagccgt ccgtcgtggg ccagggctac ccctccacgc ccggcctgca
     2101 cagcgccgag caggtcgcct cctggcgtac ggtcaccgac gcggtgcacg cgcggggcgg
     2161 caggatcttc gcccagatca tgcacgccgg ccgcatcggc cacccggtcc tgctgccgga
     2221 cggtctcacc cccgtcagcg cctccccggt caaggccgag ggacaggtct acacccacgt
     2281 cggaccgaag gacttcgtcg agccgcacga gctgaccgac gccgaggtcc gcgcgaccgt
     2341 cgccgacttc gtcaccgcct cccgcaacgc ggtggaggcc gggttcgacg gggtcgagct
     2401 gcacggcgcc aacggctacc tcatccacca gttcctcgcc cccaacacca acctgcgcac
     2461 cgacgcgtgg ggcggctccg acgaggcccg cacccgcttc gccgtcgagg tcgtcgaggc
     2521 cgtcgccgcc gagatcggcc cggagcgcac cgccctgcgc atctcgcccg gcaacccgta
     2581 caacgacatc gacgagcccg cgccggaggc cacgtacacc gcgctggtcc gcgcgatcga
     2641 gccactcggc ctcgcctatc tgcacatcct ggaggccacc ccgatccgcg agctgaccct
     2701 cgccctgcgc aaggccttct ccgggaccct cgtcatcaac gtgcactccg agggccccac
     2761 cggccccggc gaccacacgg tgatcgacga cggcatcgcc gacctgatct cctacggggc
     2821 gctcttcctc gccaacccgg acctgcccgc ccggctgaag gcgggcggcc cgttcaacac
     2881 ccccgacccg tcgaccttct tcggcggcga cgccaagggc tacaccgact acccggcgct
     2941 cgacgccgtc tgatcctcca ggggccgccc gtcccaccgc cacgcggtgc ggcgggggtg
     3001 gcccggcagt tcggcacggc cggtggccca cagcaggacg gcccaccggt cgccgcccgc
     3061 cggcgcgtcc gggaagagcc gggccagcgc ccggtcgcag agcgcggcgg gcggcgccca
     3121 ctccaggcca agaccctccg ccaggtcatg ggtgtgcacc agggtctcca ccacgcccat
     3181 cgcggcgaag ccctccgggt cggagatccc gtacccgtgg tacgagcgca ccccgggcgg
     3241 cgtcgtccgc gtcatcgagg ccagcagcgc cccgctcgcc tccagcgtgc cgagcagccc
     3301 ggccgggccg gccgcccggt cggcgaagac cgcgttgtgc ggaccgccct cccggtccgg
     3361 cgcccaccgg aagggcacct cgcggtccag cggaggcgta cggggcccca actgcaccgc
     3421 gtacgcgaac agatcgtcgc ccaggtgctc cgccgtctcc cagcaggtcc actccagcga
     3481 accggcccgg gcctcccagc ggtccgccgg ggccgtcgcc agcaggtcca ccgcgagtcg
     3541 cacggcggtc gtcacgtcgt cggcggtcac cggcagccgg gtcccgctca gcgccgcccg
     3601 ctcggccgca ctgcactcca cgcagaactc gttgccctcg gggtcggcca gcgtcgccca
     3661 gccgcgcccg ttgggcctgc ggtggtcgcc gacgagggtc gcgccgagcg cgagaagccg
     3721 ctcgacctcc tcgtcccggg agcggtcgtg cggctggatg tcgagatgga cgcggttctt
     3781 ggactgtttc ttctcggcca cctgcacgaa gaggaccgcc gcgccgggcg tctccagcag
     3841 cgcctccggg tcgccgggca ggtcctcgtc ggagagcggg gcaccgagca cctgggccca
     3901 gaagctgccc agcgcgtagg cgtcggcgca gtcgatcgtg atgtggcgta catgggaagt
     3961 catggcggac accctccccg ccgactcggt ccccggtcca gcgagttacg cggcgagccg
     4021 cccgtgcccc ggacccgtac ggcacgcgcg aaggggccgg gagggcgtac cgtcccggcc
     4081 ccttccgcct gcgcgtcgcg ctacttcacc ggggtgaagt cccgcgcccc gatgaactcg
     4141 gggcggcgga tcggagccgc gaagggctcc tgcgccgcgt tctccacgct gttgaagacg
     4201 atgaagacgt tgctgcgcgc gtacggggtg atgttgtcgc ccgagccgtg catggcgttg
     4261 cagtcgaacc aggtcgccga accggccttg cccgtgaaga gcttgatgcc gtggcggtcg
     4321 gccatcttcg tcagcgcctc gtcggacggg gtgccggcgt cctgcatctg gagcgacttc
     4381 ttgtagttgt ccttcggcgt ctcgcccgca cagccgagga acgtcttgtg cgaaccgggc
     4441 atgatcatca gcccgccgtt ggtgtcgaag ttctcggtca gcgcgatcga cacggacacc
     4501 gtccgcatgt tcggcagacc gtcctcggcg tgccaggtct cgaagtccga gtgccagtag
     4561 aagcccgtgg ctccgaagcc cggcttcacg ttgatccgcg actggtggac gtacacgtcc
     4621 gagccgagga tctggcgggc ccggcccacc accctctcgt cgcggaccag cgcggcgaag
     4681 acctcgctga tcttgtggac ctcgaagacg gaccgcacgt tctgcgactg cttctcgacg
     4741 atcgagcgct cgtctgcgcg gatcagcggg tcggagacca gccggtccag ctccgcctgg
     4801 tagacggcga cctcgtccgg cgcgatcagc tccccgatgc tgaggaagcc gtcgtgctcg
     4861 aagctctgga gatccttggc ggcgatcgga cccggtgcgc ccggcgcgga ccagatgacc
     4921 gggtcctggc ggggagtggt catctcggcg gcgccgcgcg aggggtacag atcggcgcgt
     4981 acttcggtgg tcatggtggt tcaggcctcc tcggtcagca gtgggtagac accgttctcg
     5041 tcatggtcct cccgtccggt gacgggggga ttgaagacgc acacgcagcg gaagtcggtc
     5101 ttgggccgca tggtgtgccg ctcatgcccg tccagcaggt acatggtccc gggcgtgatc
     5161 cagtgcttct cgccggtctc gtcgttggtg agctcggcct cgccctcggt gcacagcacc
     5221 gcctcgatgt ggttcgcata ccacatcgag gtctccgtgc ccgcgtacag cacggtctcg
     5281 tggagcgaga agcccacctt ctccttggcg agcacgatgc gcttgctctc ccaggtgccg
     5341 gacgcggcct tcacatgccg gtcggtgttc tcgatgtcac tgaacgatcg gacgatcacg
     5401 gtgagcggtt gcctttctct tctctgcaca gggcccgggc agcgaagccc gggccccctc
     5461 ttcctcttcg cctgccccgg cagcgccggg tcaggccgtc tcgcggacgc agcgggccag
     5521 cgtgcgcagg ccctcttcca actcctcggg ggtgatggtc agcggcggca gcagcttgac
     5581 gacctcgctc tgcgggccgg aggtctccag caggagcccc agctcgaagg cgcgggcgca
     5641 gaccgcggag gcgcgggcag ggtcctcgaa ctccatgccc cacaccaggc cccggccgcg
     5701 gaagctcgcg gtcgactcct cggcgcagat ggccagcagc gtctgctcga tctgctcgcc
     5761 gcgggccagg gtctgcttct ccatctggcc gtccgcccag tacgcctgca gcgcggcggc
     5821 ggcggtgacg aaggccgggt tgttgccgcg gaaggtgccg ttgtgctcgc ccggctccca
     5881 gatgtccagc tccggcttga acaggcagag cgacatcggc agcccgtacc cgctgatgga
     5941 tttcgacagc gtgacgatgt ccggggtgat cccggcctcc tcgaaggaga agaagccgcc
     6001 ggtacggccg cagcccatct ggatgtcgtc gacgatcagc agcatgtcct ggcggtggca
     6061 cagctcctgc agcgcccgca gccactcggc gcgggcgacg ttgatgccgc cctcgccctg
     6121 gaccgtctcg acgatcaccg cggcgggctt gttcagcccg gagccctggt cctccagcag
     6181 ccgctcgaac cacaggaagt ccgggacctg gccgtcgaag tagttgtcga acggcatcgg
     6241 ggtgccgtgc accagcggaa tcccggcccc ggcgcgcttg aaggcgttgc cggtgacggc
     6301 cagcgagccc agcgacatgc cgtggaaggc gttggtgaac gagacgacgg actcgcggcc
     6361 cttgacctta cgggccagct tcagcgccga ctcgacggcg ttggtgcccg tcgggccggg
     6421 gaacatcacc ttgtacggca ggtcgcgcgg gcgcaggatc acgttctgga acgtctccag
     6481 gaacgcccgc ttggcggtcg tcgccatgtc caggccgtgg gtgatgccgt cgcgctcgat
     6541 gtagtcgatc agcgctcgtt tcagcaccgg gttgttgtgg ccgtagttga gcgagccggc
     6601 cccggcgaag aagtccaggt aggagtggcc gtcctcgtcg gtcagccggg cgccctgcgc
     6661 gcggtcgaag acggcgggcc agccgcggca gtagctgcgg acctcggact ccagggtctc
     6721 gaagacgctc agtgcgggcg gggtgatggt cacagcgggt ctcctgcgtg agggggtgtg
     6781 acgggcggtg gtctggtgct ccccgccgca cggatgcggc cgggaccgcg ccgggtggtc
     6841 ggccggcgcg aaaagtcgtg ggggccgggc gcacgggtcg ggtgcgccgg tccggtgtcg
     6901 ggcggcgtgc gtacgggtcg ggtacgcggg gggcacgccc gggcgggctc aggtggggaa
     6961 cgggccgatc cggtagagca cctcgggcag gtgcgtctct tcggggaaca ggtcgccgtc
     7021 gaagagaacc tcctgctcca gggccacgtc gtgccgctgg gcgtaggacg tgaacagccg
     7081 gtcggacgcc gcgttgtccg gggtgatcgt cgtctccacg gacgacaggc cctggtcggc
     7141 ggcgacccgg gcggtcagtg cgtccagcag agtcgcggcc agtcccttgc cgcggtgggc
     7201 cgagtcgacg gccacctgcc agaccacgag ggtctggggg cggtcgggcc ggatgtaacc
     7261 cgtcacgaag gcgatgggct cgccgtcctc gccacgggcg accacggagg tcgcggcgaa
     7321 gtcacggcac cacagcaggt agctgtacga ggagttcagg tccaggacct gggagtcgcg
     7381 ggcaatgcgc cagatcgcgg ctccgtcctc cactcgcggc gcatcgatac tgaggaattc
     7441 gcttcgggca cgggcaaaat ctgcaggtgc ggcggtcatg aaattgaatt tacccagcaa
     7501 atctcaaaat tgcatcggcg aaaggggttg ggtggagggc gtccatgtgt tatcaggcgt
     7561 gcgcgacctc cccgtcgaat caggggtgac ttcggcacgt ttgtcctgaa tttaacgggc
     7621 ataacgccga gcttgtggag tcggtcacaa ggtcgtaacg ctctcggtac gcgcccgaat
     7681 cgtgaagtcg gatctgcggg aaaactggtg cgtttaggtg ctgaaaaagc gggcagaaga
     7741 atgcgggaag ctgttttccg attaacagct tcccgtatgg atgggtaatt tatcggctcg
     7801 gccaggtttc cgaaacagcc cggcgggcag cctcgatatc gacctgccgg cccgcatcgg
     7861 tcagcgccgc ccccagagcc gcgagcgagg acagcaccgc accccgcgtc gcatccgccc
     7921 cgtaatggtt gacccggatc atctccttgg acagcgcccc gccgcccgcg accagcggca
     7981 gcgcggggtc cgccgccagt gccgccgcga ccaacgcggc cgcgtccgtc cccgccggcg
     8041 ctcgcagcgt cgtcgccacc ggggccgcgt cccgcgccgc gtggacgtac ggctcaagac
     8101 cgccgcccag cgccaccgct ccggcccggg tcgccgcggc ggccgaggcg tggcgggcca
     8161 tcaccgtctc cagcccctcg tcctcgatcc gggccacgca cgcctccagg cccagcatct
     8221 ccagctgcgc cggggcgtgc ggcagagccg cgcggccgcc gtcgatccag cgctccttcc
     8281 agtccagcag cgagaggtag gagcggcgcg gggcggccgg gttggcggcg atccgctccc
     8341 aggcccgggc gctcaccgac accgcggaga ccccggcggg cccgcccatg gccttctgcg
     8401 cgccgatcac gcacaggtcg acgccccagg cgtccggcag cagcggctcg gcgcccaccg
     8461 aggcgaccgc gtccagcatg aacagcgcgc cgtgcgcccg gaccacctcg ccgatctccg
     8521 cgaccgggtt ggtgttgccg gtcgccgcct ccgcgtgcac cagcgagacg aagtcggtct
     8581 ccgggtgctc ggcgagcgcc tcggcgacct gctcggcggt caccgccgtg tggaagggca
     8641 ccgcgaggtc gacgacctgc gcaccgcagt cccgcagcca gttgccgaag gtctgcccgt
     8701 acgggcccgt gaccacgttc agcgcggtcg agccgggccg cgcgccactg cggatacagc
     8761 cctccagcgg cagcagcgcc tcgccctggg tgatgacgac gtcctgctcc gtggcgagga
     8821 gtcgggccac gcgccgctcg atcgccgcga agtggccggc ggtcagcggg ggaaggtcga
     8881 ggaaggggtg cgtcacggtg gggctctctt cgggttcgtc cgtcggtcga aaggcactgg
     8941 cagcctaccg acggcccacc ggcggcctcg tacagtgccg cgtatgagtg atcaggtgag
     9001 tgatcgggtg aggggcctgg cgggtggtcg gagtgcgtga cggggaggag cggccggtgc
     9061 tgcgggtgaa ggggcgggtg ctcgtcggcc cggacgaggt acgggacgaa ctgtgggccg
     9121 tggacggacg gatcacctac gagcggccgc ccggcgccga cgacgcggtg acggtcaggg
     9181 gctgggcgct gcccggcctc gtcgacgcgc actgccatgt cggtctcgac cggcacggcc
     9241 cggtcgacgc ggcgaccgcc gagaagcagg cgctgaccga ccgggaggcc ggcaccctgc
     9301 tgatccggga cgccggatcg ccctccgaca cccgctggat cgacgaccgc gaggacctgc
     9361 cgaagatcat cagggccggc cgccacatcg cgaggacccg ccgctacatc cgtaactacg
     9421 ggcacgagat cgagccgggc gacctcgtcg cgtacgtcgc ccaggaggcc cggcgcggcg
     9481 acggctgggt caagctggtg ggggactgga tcgaccgcga cgccggggac ctcaccgcct
     9541 gctggccgcg cggcgaggtc gaggcggcca tcgccgaggc gcaccggctg ggcgcccggg
     9601 tcaccgcgca ctgcttcgcc gaggagtcgc tgcgggatct ggtggaggcg ggcatcgact
     9661 gcatcgagca cgccaccggc ctcaccgagg acaccatccc gctcttcgcc gaacgcggcg
     9721 tcgcgatcgt cccgaccctg gtcaacatcg ccaccttccc cgatctcgcc gcgggcggcg
     9781 aggccaagtt cccccgctgg tcggcccata tgcggcagct gtacgaccgc cgctacgaca
     9841 cagtgcgctc cgcccacgac gccgggatcc cggtctacgt cggcaccgac gcgggcggct
     9901 ccctggcgca cggcctggtc gcgggcgagg tcgccgagct ggtcaaggcg ggcatcccgc
     9961 ccctggaggc cctctccgcg accgcgtggg gcgccaggcg ctggctgggg cgacccgggc
    10021 tcgacgaggg ggccccggcg gacctggtcg tgtacgacgc ggacccgcgc gcggacgtcc
    10081 gggtgctggc ggcgccccgg cacatcgtgc tgaacgggcg ggtcatcggc tgacccggcg
    10141 gtgtgttgac gggcagtgac ggatgcggct gtcggctcgt tggtaaagcc cggcccgttc
    10201 ccgtacctcg tccctgccgt gtgaaacggg tgacggggag aggcgggagg gaacgcacgt
    10261 gccgaaagat tcgaatatgc gcgcggaaac ccccctttgg ggtgaactca cttcaggtat
    10321 ccgccagttc accctcagtg cgtaaagatt cacggagtcg aggccaccgg cgcaggcgat
    10381 gtcccccatt ggactgtgc
//
