LOCUS       NZ_CP029361            10375 bp    DNA     linear   CON 31-MAY-2024
DEFINITION  Streptomyces globisporus strain TFH56 chromosome, complete genome.
ACCESSION   NZ_CP029361
VERSION     NZ_CP029361.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces globisporus
  ORGANISM  Streptomyces globisporus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7488586)
  AUTHORS   Cho,G.
  TITLE     Complete genome sequence of Streptomyces globisporus TFH56 isolated
            from tomato flower
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7488586)
  AUTHORS   Cho,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2018) Division of Applied Life Science, Gyeongsang
            National University, Jinjudaero, Jinju 52828, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-MAR-2018
            Assembly Method :: canu v. 1.7; arrow polish v. SMRT link 5.1.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 37.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003147545.1-RS_2024_05_31
            Annotation Date                   :: 05/31/2024 02:17:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,917
            CDSs (total)                      :: 6,829
            Genes (coding)                    :: 6,492
            CDSs (with protein)               :: 6,492
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 337
            CDSs (without protein)            :: 337
            Pseudo Genes (ambiguous residues) :: 0 of 337
            Pseudo Genes (frameshifted)       :: 240 of 337
            Pseudo Genes (incomplete)         :: 121 of 337
            Pseudo Genes (internal stop)      :: 7 of 337
            Pseudo Genes (multiple problems)  :: 31 of 337
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:15:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2096982
            Orig. end                         :: 2107357
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029361.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10375
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="12"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10375
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10375
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5375](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5375)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(1182..1901)
                     /locus_tag="DIJ69_RS09100"
                     /old_locus_tag="DIJ69_09100"
     CDS             complement(1182..1901)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /locus_tag="DIJ69_RS09100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_09100"
                     /product="HAD family hydrolase"
                     /protein_id="WP_032794409.1"
                     /sec_met_domain="HAD_2 (E-value: 2.1e-33, bitscore: 114.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAERPAILFDLDGTLLDTPGAIANTLHRVLAETGHGDVPDERVR
                     ATVGRPLTAVFADLTGSPEDSAPVAEAVALFRELFRRTVVPEAAALVFPGIPDLLRTLR
                     RSGYRTAVVTSKIRPSAMELLEPAALVDQFDAIICHGMAERGKPHPDLALLAAERIGSP
                     AHLCTVVGDAVDDMRMAAGAGMRAIGVTYGVASARQLTASGAVQVVETVADLARSLGRE
                     PSPPTAPAPRAAPVPLP"
     gene            complement(1898..2878)
                     /locus_tag="DIJ69_RS09105"
                     /old_locus_tag="DIJ69_09105"
     CDS             complement(1898..2878)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060202.1"
                     /locus_tag="DIJ69_RS09105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_09105"
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="WP_030810967.1"
                     /transl_table=11
                     /translation="MTAVTTALHRALAADPDLLVLDEEVAEALHEGRPVVALESSAIPQ
                     GPAFPANLDLALDIDAAVRASGSVPARIALLGGKILVGMDKDRLEQFATDTSIPKVSTR
                     GVGLTLAGGGAGGTTVSSSLLAADLAGIPVFAVAGIGGVHRDAPQSFDISADLVQFTRS
                     RTAVVCAGAKSMLHPAWTLEWLETAGIPVVGYRCDDFPAYLSVSTGVRNPHRIDDLREI
                     AAVAATHWQVGNETSLLVTHPISEADGIPTADIEAALADATALAEAAGVTGPAITPYLL
                     KAIAEATEGRTSAANRAVLLSTATLAGEFAVQMSLELAERAGESQ"
     gene            complement(2956..3663)
                     /locus_tag="DIJ69_RS09110"
                     /old_locus_tag="DIJ69_09110"
     CDS             complement(2956..3663)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060204.1"
                     /locus_tag="DIJ69_RS09110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_09110"
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="WP_044372119.1"
                     /transl_table=11
                     /translation="MTSPLPASAAPLDPFLHLTRPTSFLDYETDAVQEFVDHVVDDRSA
                     DRRTNAVKLYYAVRDTVDYEVYGADLSVTGLKASSTALARSGFCLHKSALYAAAVRALG
                     IPSRLVYGDVRNHLASPRLLAHIGGDVFFHGLTQVYLEGAWVKATPVFNKLLCKLYGMQ
                     ALEFDGRGDSLHHPYGQEGGAMEFVKDHGTFDDLPYEFVMASMRSSHPRFVEGDGTVSG
                     GSLADEAEPAVKA"
     gene            complement(3718..4857)
                     /locus_tag="DIJ69_RS09115"
                     /old_locus_tag="DIJ69_09115"
     CDS             complement(3718..4857)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060205.1"
                     /locus_tag="DIJ69_RS09115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_09115"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_052807712.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.6e-13, bitscore:
                     49.9, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.2e-22, bitscore:
                     78.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.8e-32, bitscore:
                     111.3, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRPPNAPPDDLFTLMTPWAERLNTDLDALDREAEFSHEKWSAVR
                     ESGILRTPFDTEHGGHGRSLTDTMRVLEGLGHVSRDSGLSFSTSTQMVSVGVPLQRFGS
                     AELKGRHLPGILSGETITAHAITEESGGSDAMNTATTAVRDGDHYVVDGGKMFITNAPV
                     AGLFLLYVRTGDPGPFGLTCLLVEAGTPGLTVGPAMDKIGLRTSPIGTLDFRGLRVPVA
                     QRVGKEGAGFLVLDYVMKREVLFAFSITLGEMTRRLEETIAFARKREQFGKPIGSYQAV
                     SHKIANMSIEVETARKWLRDTGAKVEERQDASLDLASTKTVVSEANVRTALDAVQIHGG
                     RGVLTAHGVERDLRNSIAGTIYSGTSEIQRNRIAALLGL"
     misc_feature    complement(4817..4832)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.35"
                     /tool="antismash"
     gene            complement(5001..5375)
                     /locus_tag="DIJ69_RS09120"
                     /old_locus_tag="DIJ69_09120"
     CDS             complement(5001..5375)
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452967.1"
                     /locus_tag="DIJ69_RS09120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_09120"
                     /product="ectoine synthase"
                     /protein_id="WP_010060207.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.6e-29, bitscore:
                     100.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIIRSVDDVVPVQWGNGTSHRLLTQADEMGFAVAVTLVRAGTTSA
                     LQYRNHLEACFCVAGSGEVVEKDGTHHKILPGMIYALDQHDPHYLVASDDEDLHLISVF
                     NPPIVGNEKHTLSEDGFSQY"
     misc_feature    5361..5376
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.75"
                     /tool="antismash"
     gene            complement(5433..5699)
                     /locus_tag="DIJ69_RS09125"
                     /old_locus_tag="DIJ69_09125"
     CDS             complement(5433..5699)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1147: putative acyl carrier protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060209.1"
                     /locus_tag="DIJ69_RS09125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_09125"
                     /product="acyl carrier protein"
                     /protein_id="WP_030810959.1"
                     /transl_table=11
                     /translation="MTSTDIAAEIHEYIVATFLDGEDCADLTHDYDLIANGVIDSLGLV
                     RMVSHIGRLYELPVDELDFGPADFRSIAAITAFIGANSRALAA"
     gene            complement(5696..7162)
                     /locus_tag="DIJ69_RS09130"
                     /old_locus_tag="DIJ69_09130"
     CDS             complement(5696..7162)
                     /NRPS_PKS="Domain: AMP-binding (12-389). E-value: 8e-68.
                     Score: 220.8. Matches aSDomain:
                     nrpspksdomains_DIJ69_RS09130_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060210.1"
                     /locus_tag="DIJ69_RS09130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_09130"
                     /product="AMP-binding protein"
                     /protein_id="WP_030810956.1"
                     /sec_met_domain="AMP-binding (E-value: 5.3e-68, bitscore:
                     228.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSPQLVDAVLAHGENRPERVALVSHRRTVTYRELADTVRTFRHR
                     LGRLGPVAGNTVCVPAHKSPETLALMLAAFREGLVVLAPSPDLGGAARDRLCRQARASH
                     LLAVTDNGVISAEPVAQDAADASRFGTPDPGITRLLLTTSGSTGTPKIVPIPLDGFAAF
                     ADWAARDFGLTADDTSLSYAPLNFDLALLDVWTFLREGARVVLVDQDRAADGAHLRHLV
                     AAHTVTFVQGVPLLHRLLTGDGTAFPTVRQVVTTGEALGPDLLRPLAAAFPGARFHNVF
                     GCTETNDSFIHPFSPATCEGRLPIGRPVEGVRAELLTPEGKRLSGPGMGELLVTTPFQT
                     SGYLESSLNAKAFTEIEGHPYYRTGDLVTRGTDGLLYLEGRADWQVKIRGVRTNLLEVE
                     TVIASHPDVTEAVVVALPDEQAGVRLHAQVVRRPTSSLTGLGLRAHAGRHLPRHAIPSS
                     VRVTEAPLPRTSTGKPDRGLIKADRLKESA"
     CDS_motif       complement(5966..6025)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DIJ69_RS09130_0002"
                     /evalue="1.40E-06"
                     /label="NRPS-A_a8"
                     /locus_tag="DIJ69_RS09130"
                     /protein_end="399"
                     /protein_start="379"
                     /score="20.8"
                     /tool="antismash"
                     /translation="GRADWQVKIRGVRTNLLEVE"
     aSDomain        complement(5996..7126)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DIJ69_RS09130_AMP-binding.1"
                     /evalue="8.00E-68"
                     /label="DIJ69_RS09130_AMP-binding.1"
                     /locus_tag="DIJ69_RS09130"
                     /protein_end="389"
                     /protein_start="12"
                     /score="220.8"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="HGENRPERVALVSHRRTVTYRELADTVRTFRHRLGRLGPVAGNTV
                     CVPAHKSPETLALMLAAFREGLVVLAPSPDLGGAARDRLCRQARASHLLAVTDNGVISA
                     EPVAQDAADASRFGTPDPGITRLLLTTSGSTGTPKIVPIPLDGFAAFADWAARDFGLTA
                     DDTSLSYAPLNFDLALLDVWTFLREGARVVLVDQDRAADGAHLRHLVAAHTVTFVQGVP
                     LLHRLLTGDGTAFPTVRQVVTTGEALGPDLLRPLAAAFPGARFHNVFGCTETNDSFIHP
                     FSPATCEGRLPIGRPVEGVRAELLTPEGKRLSGPGMGELLVTTPFQTSGYLESSLNAKA
                     FTEIEGHPYYRTGDLVTRGTDGLLYLEGRADWQVKIR"
     CDS_motif       complement(6698..6745)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DIJ69_RS09130_0001"
                     /evalue="1.50E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="DIJ69_RS09130"
                     /protein_end="155"
                     /protein_start="139"
                     /score="14.7"
                     /tool="antismash"
                     /translation="LLTTSGSTGTPKIVPI"
     gene            7666..8988
                     /locus_tag="DIJ69_RS09135"
                     /old_locus_tag="DIJ69_09135"
     CDS             7666..8988
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886446.1"
                     /locus_tag="DIJ69_RS09135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DIJ69_09135"
                     /product="transposase"
                     /protein_id="WP_078955553.1"
                     /transl_table=11
                     /translation="MSRAVMSGRAAGPGLGRLEVVEPTDTVTELLATLISPVLRRRDQR
                     RRAEQYVRGLLSVDGRKSVRRIASSVGGGAAEQGLHHFVAHSTWEWNAMRERLARHVQG
                     VMPPQAWVVQPVSIPKVGVHSVGVHRRFVPHLGQADSGQQAFGVWFAAEDMSVPVSWRL
                     HLPGDWVHDEEHRRRADIPASVVEETLEECASASVLGAVLGWRVPRAPVVMSAAGPDVG
                     GTMQRHRAAGQPLLLRISGTTGLTVADPSMPGHGAGRVSAHRIAASVRALRRPVGWPVP
                     GGAPGEAGRSSVVAVRVVLPAPGPRASRADAGRPTGLLLAEWLDPDGPPAGFWLTEMAD
                     TPVPALVRLARLADRVAGDFATLGDRVGLRDFEGRSFTGWHRHVTLASIAYAAIALGGR
                     DPWSGPERLGGPGPTVGFGAPAGLSGPTGPDGVEALFAPTG"
     gene            complement(9157..10071)
                     /locus_tag="DIJ69_RS35290"
     CDS             complement(9157..10071)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /locus_tag="DIJ69_RS35290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_244219277.1"
                     /transl_table=11
                     /translation="MTGGAEHLGTLVVHGPGPAEDDRQRLMALTAQTVAVLMLMQRSTV
                     VAEAHVRDDFFHELLASQLSPEQLATRARRLGVNLAQPHVVVVARPEGGAQGRAVVWAS
                     SYVHQLSGLKYVDGEHIVLLVPGDDPTAAGRTVSRELSVVLAHPVTAGAAGPVTALGAV
                     RSTYQEAQRCLDALTALGGTGGAASQRELGFLGLLLSERHDVGGFITSTLGPVLDYDSQ
                     QATELLRTLEEYFTSGNSPTRAAETLHVHPNTVSRRLERITYLLGSDWQEPARALEVQL
                     ALRLQRARQVLEHEGGPPSRPGP"
     misc_feature    complement(9895..9897)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(10353..10367)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.27"
                     /tool="antismash"
     misc_feature    10354..10368
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.52"
                     /tool="antismash"
ORIGIN
        1 aggtggagag gcccagcagg agcagtcccg ccgcaccgat ggcgaaggcg agggtcagcg
       61 tgcgccgtac gtccaggatg ttcaggagtc ggccggcgag gacgttgccg agggtgatgc
      121 aggcggccag cggcaggaag gcgatgcccg cggccagcgg ctcgtacccg cggccgtcct
      181 ggaggagcag ggtgacgagg tagaactcga cgccggccac gctcgccatg tagagggcgg
      241 aggagccgca gcccacgacc aggctgcggg tgcgccggag caccggtgcc accagcggct
      301 gtcggctggt tcgttcccgt acgacgaaca gggcgagcag cgccgcgccc accgcgaggc
      361 tgatcagggt ggcgggggcg gtccagcccg agtccgcggc cagcgtcagg ctcagggcga
      421 agaccaggac cgtggcggtg cagagcacgg cgttgacgat gttcagcggg acacgcgtgc
      481 cgccctcgcg gcgtccgggc agcagccggg gggcggccag cgcgcacacc acggcgaacg
      541 gcaggttcac gaagaaggtc cagcgccagg acaccgtggt cagcagcccg ccgatgacga
      601 cccccgcggc cagcccgctc gcgcccaccg cgccccacac cgcgagagcc cgggtgcgtt
      661 cccggcctgc cgggaaggtg gtgttgacca gggccaggat ggcgggctgg agtgccgcgg
      721 cggcgacgcc ctgggcgccg cgggcgatca gcagcaggga ggcgtccccg gcgagtccgc
      781 ccaggagtga ggagacgccg aacaggacga gcgcgccgat gaacatgttg cgtgcgccga
      841 gccggtcgcc gatccggcct ccggcgatca ggaatccggc gaagaacacc gagtaggcgc
      901 tgacgaccca ttggacggcg ctgccggaca ggttcagctc cgcgcccatg ctgggcaggg
      961 cgacgaagat gacggagaag tcgagggcga tgaggaactg ggccgcggag agggtgagca
     1021 gccctccggt caggcttcgc tcggacttcg ccgtcgcggg aggggggtcg gcgtgctgca
     1081 tggtgagcca cttccgttcg tgcgtgggat gcgtgggggc ggatgcgtgt gcggggttcg
     1141 tgcgggggat tcgtgcgggg ggaacaggaa atgagggggt gtcaggggag cggtacgggg
     1201 gccgcgcgcg gggccggggc ggtgggcggc gagggttccc ggcccaggct gcgggccagg
     1261 tccgcgaccg tttccaccac ctgtacggcg ccggaggcgg tcagctgacg cgccgacgct
     1321 acgccgtagg tgacgccgat ggcccgcatg ccggcgccgg cggccatccg catgtcgtcg
     1381 acggcgtcgc cgaccaccgt gcagaggtgg gcggggctgc cgatccgttc cgccgcgagg
     1441 agcgccaggt ccgggtgcgg tttgccgcgc tccgccatgc cgtggcagat gatggcgtcg
     1501 aactggtcga ccagggccgc cggttcgagc agttccatgg cgctggggcg gatcttgctg
     1561 gtgacgacgg ccgtccggta gccgctccgg cgcagggtgc gcaggaggtc ggggataccg
     1621 gggaagacca gcgccgcggc ctccggcacc acggtccggc ggaacagctc ccggaagagg
     1681 gccacggctt ccgccaccgg tgcgctgtcc tccggactgc ccgtcagatc ggcgaagacc
     1741 gcggtcagcg ggcggcccac ggtggcacgg acacgctcgt ccggcacgtc gccgtggccg
     1801 gtctcggcca ggacccggtg cagggtgttc gcgatggctc ccggggtgtc cagcagggtg
     1861 ccgtcgaggt cgaagaggat cgcggggcgc tcggcgctca ctgggactcc cccgcgcgct
     1921 ccgccagttc gagggacatc tgcacggcga actcaccggc gagggtggcg gtcgacagca
     1981 gcaccgcgcg gttggcggcg gaggtgcgcc cttcggtcgc ttcggctatc gccttgagaa
     2041 ggtacggcgt gatggcgggg ccggtcactc ccgcggcctc cgcgagggcg gtggcgtccg
     2101 ccagagccgc ctcgatgtcc gcggtcggga tgccgtccgc ttcgctgatg gggtgggtga
     2161 cgagcaggga ggtctcgttg ccgacctgcc agtgggtggc cgccacggcg gcgatctcac
     2221 ggaggtcgtc gatacggtgc gggttgcgga ctccggtgct cacgctgagg taggcgggga
     2281 agtcgtcgca gcggtagccg acgacgggga tccccgcggt ctccagccat tccagggtcc
     2341 acgccgggtg cagcatcgac ttggcccccg cgcagaccac ggcggtgcgg ctgcgggtga
     2401 actgcaccag gtcggcggag atgtcgaagc tctgcggggc gtcccggtgg acgccgccga
     2461 tgccggccac ggcgaacacc gggatgcccg cgaggtcggc cgccagcagg gaggaggaca
     2521 ccgtcgtccc gccggcgccg ccgcccgcca gggtgaggcc cacgccccgg gtgctgacct
     2581 tggggatgga ggtgtcggtg gcgaactgct ccaggcggtc cttgtccata ccgacgagga
     2641 tcttgccgcc gagcagggcg atccgggcgg ggaccgagcc cgaggcgcga acggccgcgt
     2701 cgatgtcgag tgccaggtcg aggttggccg ggaaggccgg gccctgcgga atggccgagg
     2761 actccagggc gacgaccggc cgtccctcgt gcagcgcctc ggccacctcc tcgtcgagga
     2821 ccaacaggtc ggggtctgcg gcgagggccc ggtgcagggc ggtggtcaca gcggtcatgg
     2881 tgggactcct tgggatgaca agtcgttctc gaaggcgggg agttgcatgg atccgggcgt
     2941 gcgaggtccg gggattcagg ccttgacggc gggctcggcc tcgtcggcca gcgagccgcc
     3001 cgagaccgtc ccgtccccct cgacgaaccg ggggtgggag ctgcgcatgg aggccatgac
     3061 gaactcgtag ggcaggtcgt cgaaggtgcc gtggtccttc acgaactcca tggccccgcc
     3121 ctcctggccg tacgggtggt ggaggctgtc gccacgaccg tcgaactcca gcgcctgcat
     3181 gccgtacagc ttgcagagca gcttgttgaa gaccggggtg gccttcaccc aggcgccctc
     3241 caggtagacc tgggtcaggc cgtggaagaa gacgtcgccg ccgatgtggg cgagcaggcg
     3301 gggcgaggcg agatggttgc ggacatcgcc gtagaccagc cgggagggga tgcccagggc
     3361 tcgtaccgcc gccgcgtaca gggcggactt gtgcaggcag aagccggagc gggcaagcgc
     3421 ggtgctggag gccttgaggc cggtgacgga caggtcggcg ccgtagacct cgtagtcgac
     3481 ggtgtcgcgg accgcgtagt acagcttcac cgcgttggtg cggcggtcgg cggagcggtc
     3541 gtcgaccacg tggtcgacga actcctggac cgcgtccgtc tcgtagtcga ggaaggaggt
     3601 cggccgggtg aggtggagaa acgggtcgag cggggcggcg gatgcgggca gaggggaggt
     3661 catggcggtc ccgtgggtcg ggcggcgcga ggccgggtgg caggggcggg ggccgggtca
     3721 gaggccgagg agcgcggcga tgcggttgcg ctggatctcg ctggtgccgg agtagatcgt
     3781 tccggcgatg gagttgcgca ggtcgcgctc gacgccgtgg gcggtgagga cgccgcgtcc
     3841 gccgtggatc tgaacggcgt cgagcgcggt acggacgttg gcctcggaga ccaccgtctt
     3901 ggtcgaggcg aggtcgaggg aggcgtcctg ccgctcctcc accttggcgc cggtgtcgcg
     3961 cagccacttg cgggcggtct cgacctcgat gctcatgttg gcgatcttgt gggacacggc
     4021 ctggtagctg ccgatcggct tgccgaactg ttcgcgcttc ctggcgaagg cgatcgtctc
     4081 ctccaggcgc cgggtcatct cgcccagggt gatggagaag gcgaacagca cctcgcgctt
     4141 catcacgtag tccaggacca ggaatcccgc cccttccttg ccgacccgct gggcgacggg
     4201 aacccgcagg ccacggaagt ccagggtgcc gatcgggctg gtgcgcagcc cgatcttgtc
     4261 catggcggga cccacggtca gaccgggtgt gcccgcctcg acgagcaggc aggtgaggcc
     4321 gaacgggccg gggtcgcccg tgcggacgta gagcaggaac aggccggcca cgggcgcgtt
     4381 ggtgatgaac atcttgccgc cgtcgaccac gtagtggtcg ccgtcccgta cggcggtggt
     4441 cgccgtgttc atcgcgtcgg agcctccgga ctcctcggtg atggcgtgcg ccgtgatcgt
     4501 ctcgccggag aggatgcccg gcaggtgacg gcctttcagt tccgcgctgc cgaaccgctg
     4561 gaggggaacg cccacggaga ccatctgggt ggaggtggag aaggagaggc cggagtcgcg
     4621 ggacacgtgc ccgagcccct ccagtacccg catggtgtcg gtcagcgacc ttccgtggcc
     4681 gccgtgttcg gtgtcgaacg gggtgcgcag gatgccggac tcgcggacgg cggaccactt
     4741 ctcgtgggag aactccgctt cccggtccag cgcgtcgagg tcggtgttga gccgttcggc
     4801 ccagggggtc atcagggtga agagatcgtc ggggggcgcg ttcggggggc gggacatgag
     4861 gttccttcgt caggaggggc gagggtccgt cggggcgcac ggcatgaggg tgcgcggcgc
     4921 cccgacggcg gggaggcagg gcgcggccgg cccctggcgg gacgcggtgg gtgcggcatc
     4981 ggccggggag ggcgtacggc tcagtactgg gagaagccgt cctcggagag cgtgtgcttc
     5041 tcgttgccga cgatcggcgg gttgaagacc gatatgaggt gcagatcctc gtcgtcggag
     5101 gccaccaggt agtgggggtc gtgctggtcg agggcgtaga tcatgccggg caggatcttg
     5161 tggtgggtgc cgtccttctc gacgacctcg cccgagccgg ccacgcagaa gcaggcctcc
     5221 aggtggttgc ggtactggag ggcgctcgtg gtgcccgctc gcaccagcgt gacggcgacg
     5281 gcgaagccca tctcgtccgc ctgggtgagc agtcggtggc tggtgccgtt gccccactgg
     5341 acgggaacga cgtcgtcgac actgcgaatg atcatgagga actccttgga aagtgaggaa
     5401 gaaggaccgg gggtgcggag gagccggcgg ggtcaggccg ccagggcgcg cgagttggcg
     5461 ccgatgaacg cggtgatggc ggcgatgctg cggaagtcgg cggggccgaa gtccagctcg
     5521 tcgaccggaa gttcgtagag gcgtccgatg tgggagacca tccggaccag acccaggctg
     5581 tcgatcactc cgttggcgat caggtcgtag tcgtgggtga ggtccgcgca gtcctcgccg
     5641 tcgaggaacg tggccacgat gtactcgtgg atctcggccg ctatgtcggt actggtcatg
     5701 ctgactcctt gaggcgatcg gccttgatga ggccgcggtc gggcttgccg gtggacgtgc
     5761 ggggcagagg ggcctcggtg acgcgcaccg aggacgggat ggcgtggcgg ggcaggtggc
     5821 ggccggcgtg ggcgcgcagg cccagtccgg tgagggagct ggtggggcgg cgcaccacct
     5881 gggcgtgcag gcggactccg gcctgttcgt cggggagggc gaccaccacc gcctccgtga
     5941 cgtcggggtg ggaggcgatg acggtctcca cctccaggag gttggtgcgc acgccgcgga
     6001 tcttgacctg ccagtcggcg cggccctcca ggtacagcag gccgtccgtg ccacgggtga
     6061 cgaggtctcc cgtgcggtag taggggtgac cctcgatctc ggtgaacgcc ttcgcgttga
     6121 gggaggattc gaggtagccg ctcgtctgga agggggtggt gaccagcaac tcccccatgc
     6181 cggggccgga gagccgcttc ccttcggggg tgagcagttc ggcgcgtacg ccctcgaccg
     6241 ggcggccgat gggcagccgg ccctcacagg tggcgggtga gaaggggtgg atgaaggagt
     6301 cgttggtctc cgtgcagccg aacacgttgt ggaagcgcgc cccggggaac gccgcggcca
     6361 gcgggcgcag caggtccggc ccgagcgcct cgcccgtggt gacgacctgg cggacggtgg
     6421 ggaaggctgt gccgtcaccg gtcagcagcc ggtgcagcag gggcacgccc tggacgaagg
     6481 tgacggtgtg cgcggcgacc aggtggcgca ggtgggcgcc gtcggccgcg cggtcctggt
     6541 cgaccaggac gacccgggcc ccttcccgca ggaacgtcca gacgtccagc agggcgaggt
     6601 cgaagttgag cggggcgtag gagaggctcg tgtcgtccgc cgtgaggccg aagtcccgtg
     6661 cggcccagtc cgcgaaagcg gcgaagccgt cgaggggaat gggcacgatc ttgggtgtgc
     6721 ccgtcgagcc cgaggtggtg aggagcagcc gggtgatccc ggggtcgggc gtgccgaacc
     6781 gggaggcgtc ggcggcgtcc tgggcgaccg gctcggccga gatgacaccg ttgtcggtga
     6841 cggccagcag gtgggaggcg cgggcctgcc ggcacagccg gtcccgcgcc gcgccgccca
     6901 ggtccggaga gggcgccagc accaccaggc cttcgcggaa ggccgcgagc atcagggcga
     6961 gggtctccgg ggacttgtgg gccggaacgc acacggtgtt tcccgcgacc ggtcccagcc
     7021 ggccgagccg gtggcggaac gtgcgtacgg tgtcggccag ttcccggtag gtgacggtgc
     7081 gccggtggct caccagcgcg accctttcgg gtctgttctc gccgtgggcg agaacggcat
     7141 cgacgagctg cggtgacggc atggaggttc ccctgtcgga tcggatcgcg tggagcggtg
     7201 ggaagcggcg cttcggtgct gtacagcggg gcgacggtgc cgcgctgcgg agcaggtcgt
     7261 gcggacgttc tcccgtgacc tctcccctgc ggtaccgagc gggccgggtc gtcgggcccg
     7321 gcgctgcggt gggttgttcg gtcatcacca tgcgaagcga gcggaagccc tcacaagcgg
     7381 tgtcctggct tctcgtcagg tccgccggac tccgtcagat acgccgactt cgtcaggtcg
     7441 cacgccgggg gtgcgcgggg gcggcatgtg ccccggggtg gtgcccccac cacacctcca
     7501 ggcctcccgg gagtccgcag gaccggctcg gaccggcccc catggtggtc cggccaccac
     7561 ccgcacgccg gatggggtcg tcgtgacacc tctgcccatg accgccgggg cgggcagagt
     7621 gacggtcatc gggagaggca gcgaacagga cagggcggga gaggcatgag cagagcagtg
     7681 atgtcgggcc gcgcggccgg accgggtctc ggccggctgg aggtcgtgga gccgacggac
     7741 accgtcaccg agctgttggc cacgctgatc tccccggtcc tgcgccgcag ggaccagcgc
     7801 aggcgggccg agcagtacgt acggggtctg ctgtcggtgg acggccgcaa gtcggtgcgc
     7861 aggatcgcca gcagtgtcgg cgggggcgcg gccgaacagg gcctgcacca cttcgtcgcg
     7921 cactccacct gggagtggaa cgccatgcgt gagcggctcg cccgtcacgt ccagggggtg
     7981 atgccaccac aggcgtgggt ggtgcagccg gtgtccatcc cgaaggtggg cgtccactcg
     8041 gtgggggtgc accgccggtt cgtccctcat ctgggccagg ccgacagcgg ccagcaggcg
     8101 ttcggtgtct ggttcgccgc cgaggacatg agcgtgccgg tcagctggcg gctgcacctg
     8161 ccgggtgact gggtgcacga cgaggagcac aggagacggg ccgacatccc cgcttcggtg
     8221 gtcgaggaga cgctggagga gtgcgcttcg gcctcggtgc tgggcgccgt gctcggttgg
     8281 cgggtgccga gggcgcccgt ggtgatgagt gccgcgggcc ctgacgtggg gggcacgatg
     8341 caacgtcacc gcgccgccgg acagccgttg ttgctgcgga tctccgggac gacggggctg
     8401 accgtggccg acccgtcgat gcccgggcac ggtgcggggc gggtgagcgc gcaccgtatc
     8461 gccgcgtcgg tacgggcgct gcgccgcccc gtcgggtggc cggtgccggg cggcgcaccg
     8521 ggcgaggccg gccgctcctc ggtggtggcc gtgcgcgtgg tgctgcccgc cccggggccg
     8581 cgggcctccc gcgcggacgc cgggcggccg acggggctgc tgctggcgga gtggctcgat
     8641 cccgacggac cgccggccgg gttctggctc accgagatgg ccgacacccc ggttccggcg
     8701 ctggtgcggc tggccagact ggccgaccgg gtggccgggg acttcgcgac gctgggcgac
     8761 agggtcgggc tgcgggactt cgagggccgg tccttcacgg gatggcaccg tcatgtcacg
     8821 ctcgcctcga tcgcgtacgc ggccatcgct ctcggcggcc gtgacccatg gtcggggccg
     8881 gagcgtctcg gtgggcccgg gccgacggtc ggcttcggag cgccggcggg tctgagcggg
     8941 ccgacggggc cagacggggt cgaggcgctc ttcgcaccga ccggctgagg ccgatgccga
     9001 gggtccggcg gcctggaggt caccgcggcg cgccgccggt cgtcagggat gcgttgcggt
     9061 acgcggcgcc gctgtcatca gggacgcgtt gcggtacgtg gcaccgctgt catcagggac
     9121 gcgttgcggt acgtggcgcc gctgtcgtca cggtgctcac gggcccggac gggaaggcgg
     9181 tccgccctcg tgctccagca cctggcgagc gcgttgcagg cgcagggcga gttgcacctc
     9241 cagggcgcgg gccggctcct gccagtcgct gccgagcagg tacgtgatgc gttccaggcg
     9301 ccgcgacacg gtgttggggt ggacgtgcag cgtctcggcg gcccgggtcg ggctgttgcc
     9361 cgaggtgaag tactcctcca gggtccgcag cagctcggtc gcctgctgac tgtcgtagtc
     9421 cagcacgggg cccagcgtcg aggtgatgaa tccgccgacg tcatgccgct ccgacaacag
     9481 cagccccaga aagccgagtt cccgctggga ggcggctccg cccgtgccgc ccagagcggt
     9541 cagtgcgtcc aggcagcgct gtgcctcctg gtaggtgctc cgcacggctc ccagggcggt
     9601 gaccggtccg gcggctcccg cggtgacggg gtgggcgagc accacggaca gttcccggga
     9661 cacggtccgc ccggcggcgg tgggatcgtc gcccggcacc agcagcacga tgtgttcccc
     9721 gtcgacgtac ttgagtcccg acagctgatg cacgtaggac gaggcccata cgaccgcccg
     9781 gccctgcgcg ccgccttcgg gacgggccac cacgacgaca tgcggctgag cgaggttgac
     9841 gccgagccgg cgggcacggg tcgccagttg ttccggggag agctgggagg cgagtaactc
     9901 gtggaagaag tcgtcgcgga cgtgtgcctc ggccaccacg gtgctgcgct gcatgagcat
     9961 cagtacggcg accgtctggg cggtgagcgc catcagccgt tgccggtcgt cctcggccgg
    10021 ccccggcccg tggacgacga gggtgcccag gtgctccgcg ccgccggtca ccgcgtggac
    10081 ccacacctcg gtgtcgtcgt ccgccgtgct ctcgcagcgg atcggctctc tgcgcgcgta
    10141 ggagtccagg gagggcccgt acgagtgcgg cctcgtcgac ggagagcggc cctcgtccgg
    10201 tggtgccgtc cccggcttcc gtaccggcgc tcctggctgc ggacgaccgg gcggcggacg
    10261 ctttcggcgc gctcggtgaa ccgccgcgcg cctggccggt ggcgggcgcc gggaatcccg
    10321 cggtccgttc cagatccttg ccgaccgcgt cgcggaccat cagttccgag ccgag
//
