LOCUS       NZ_CP029254            11989 bp    DNA     linear   CON 23-OCT-2024
DEFINITION  Streptomyces spongiicola strain HNM0071 chromosome, complete genome.
ACCESSION   NZ_CP029254
VERSION     NZ_CP029254.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces spongiicola
  ORGANISM  Streptomyces spongiicola
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7180417)
  AUTHORS   Zhou,S. and Huang,X.
  TITLE     Complete genome sequence of the Type Strain of Streptomyces
            spongiicola HNM0071, the producer of staurosporine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7180417)
  AUTHORS   Zhou,S. and Huang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2018) Institute of Tropical Agriculture and
            Forestry, Hainan University, No 58, Haikou, Hainan 570228, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 850.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003122365.1-RS_2024_10_23
            Annotation Date                   :: 10/23/2024 01:04:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,921
            Genes (coding)                    :: 5,739
            CDSs (with protein)               :: 5,739
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 47 of 182
            Pseudo Genes (incomplete)         :: 161 of 182
            Pseudo Genes (internal stop)      :: 9 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:10:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6451496
            Orig. end                         :: 6463485
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029254.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11989
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="24"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11989
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11989
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6989](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6989)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            62..871
                     /locus_tag="DDQ41_RS28120"
                     /old_locus_tag="DDQ41_28120"
     CDS             62..871
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1292: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049582.1"
                     /locus_tag="DDQ41_RS28120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_28120"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_109296978.1"
                     /transl_table=11
                     /translation="MTGQDPAAVRIDTGKPHPARMYDWFLGGKDNYPVDEEMARQLLEL
                     DARGRDMARVNRAFMHRATRWLGGNGIRQYLDVGTGIPTEPNLHQIAQQVAPDSRVVYC
                     DNDPIVLAHAEALLRSTPEGATRYIQADARRPGAIIEAAGEVLDFDRPVALSLLALLHF
                     VDDEDGAYELVNRLVGRLAPGSYLVLSHTTGDFDPEKAAVARAMYKERGLTLRPRSRDE
                     FTRFFDGLDLVAPGVSLTAEWHPGLGEAVAVTGDEPIPGYAALARVR"
     gene            1495..3906
                     /locus_tag="DDQ41_RS28125"
                     /old_locus_tag="DDQ41_28125"
     CDS             1495..3906
                     /EC_number="7.1.2.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0120029 - proton export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019359601.1"
                     /locus_tag="DDQ41_RS28125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_28125"
                     /product="plasma-membrane proton-efflux P-type ATPase"
                     /protein_id="WP_262508600.1"
                     /transl_table=11
                     /translation="MRDRRTDEDRRADERRAADAGLDAAQAAARRQRYGPNVLEEERRS
                     VLLEFLSHFWGPIPWMIEAALVLTAVTRRWLDFAIILALLLLNGLVGFWEEHQARGAIA
                     ALRKRLAKVAQVKRDGQWTTLPASELVPGDLVRVVRGQVVPADGTVVEGEGEVDESALT
                     GESLPVAKNPGDGMYSGSAVSRGGPVVRVLATGADTEFGRTAQLTGRAAPVSHFQRAVV
                     NIGKYLSLLALALVAVIVVLSLLRGSGVTRTMEFALVVVIASIPVALPAVLSVTMAVGA
                     RYLAGREAVVSHLPAVEEMAGVDVLCADKTGTITRNELAVADVVVLREGTDEGRVLLQA
                     ALTAEPGGGDPVDEAVLEACGAERLEGWEVTDFTPFDSARKFARAEVRGPGAERAVAKG
                     AVQAVLGLAEADASVAQRVEEVTRGFAGRGFRAMAVACADGGEWRVTGMLGLQDPPRED
                     SRATLEEAGRLGVQVRMVTGDRVEIAREICGRVGMGTDVLDAGRIEHLQGDDLARAVER
                     ADGFAQVVPEDKYRIVKALQEHGHIVGMTGDGVNDAPALQRADAGIAVFGATDAARAAS
                     DIVFLSPGLSTIIEAVHRSREVFRRMKNYAIYRITETIRVVLFVTATIVVFAFFPVTPI
                     QVVLLAILNDAAILSIAYDRVRPSSRPERWDLTEVMAVAAVLGLFGLVSTFVLVWVTHL
                     PLGLADADVRTLIYLKLSVSGHLTVFLARTRGPFWSYRPSWILLTAVIGTQILATAIAL
                     TGFLMHPIGWSLVGLVWGWAFAEFLLLDPVKLMAYRVLERHGKAAKAVSS"
     misc_feature    complement(1500..1520)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.37"
                     /tool="antismash"
     gene            complement(4183..4878)
                     /locus_tag="DDQ41_RS28130"
                     /old_locus_tag="DDQ41_28130"
     CDS             complement(4183..4878)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088744.1"
                     /locus_tag="DDQ41_RS28130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_28130"
                     /product="DUF5825 family protein"
                     /protein_id="WP_109296980.1"
                     /transl_table=11
                     /translation="MTSAVHPPNTAAGGIPLTVTAWRDYDPDACALPGMALGSHRLTGP
                     MGEETERLWGLGARRVTLSRTVDLTPAANTAPAAARRTVRSLCLVRDLTARAVLVEWRL
                     RVGPGDDETWKLLSHLQPPQRLEGAKGAQEQLIAWRGTHYLCKCLWRQGPGFLQIRDRR
                     WGDLRRFTCDEPHYHEAIARLDPGAPAADVAPDALADFTEEHLVLRVGDLAWWLPYRVK
                     RWIQEAMAV"
     gene            complement(5001..6989)
                     /locus_tag="DDQ41_RS28135"
                     /old_locus_tag="DDQ41_28135"
     CDS             complement(5001..6989)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606751.1"
                     /locus_tag="DDQ41_RS28135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_28135"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_109296981.1"
                     /sec_met_domain="TIGR03975 (E-value: 1e-185, bitscore:
                     617.0, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILKRSVDERVPGAAAEVLHANLEF
                     TDWITARRGFTADDYQYYSLSSYFMGCGDWVFSSALYDDPSWREEEFASVMKGKLRGSR
                     MRMTRELHREVPQFVEEIARRIVGLAPDVVGFTSTFQQNTAALAAARHVKRLAPHIATV
                     VGGANCDAEQGAAVHRNFPFVDYVVRGEGETAFPELVRALAGGRTGLDAVPGLCHRAPD
                     GTSTVNPMATAPLPPAHILPPDYSGYFERLAGSVARNWVEPKLVVEGARGCWWGEKHHC
                     TFCGLNGSFMQFRSKSPEVFYEEIMDLARRHRVLDMYVVDNILDMGYLGTVLPRIIESG
                     FDLRLHIEIKANMRRSQLRTLSEAGLIYVQPGIESLNNRVLDLMDKGVSGCQNVRMLRD
                     GAETGLSVSWNYLHGFPGETADDYEPVIAQIPALEHLDPPVDLSARIAIERFSPYFNRP
                     ELGFTGLRPEAHYRFTYDLPEEELYDLAYVFEAPERGIGEPTVTALNDALAGWKRHHAD
                     ARLTHTDLGDRIVLVSRRRAFPWRAMELTEPFEIALFRLLDQPHAPAALARKAAARVPG
                     DPRGREEVERVLDEWVARGIVFTDGGQYVHVAPAAVNEDLLRLDFMRHLHTAGAGAPRE
                     AGGGGSGAAARTPADRDRGPAQPPVTV"
     gene            complement(7380..9464)
                     /locus_tag="DDQ41_RS28140"
                     /old_locus_tag="DDQ41_28140"
     CDS             complement(7380..9464)
                     /NRPS_PKS="Domain: Abhydrolase_1 (410-549). E-value:
                     3.3e-10. Score: 31.9. Matches aSDomain:
                     nrpspksdomains_DDQ41_RS28140_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569707.1"
                     /locus_tag="DDQ41_RS28140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_28140"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_262508601.1"
                     /sec_met_domain="PF00561 (E-value: 5e-08, bitscore: 31.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 4.8e-34, bitscore:
                     116.1, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRPPTGNGPHRPVRAPASAAPRAAADGGGGEAPGPRFRTYRAEL
                     PDVCAADPARMVFTADADGRCQVFTWDATTGRAEQVTDRPGGTLRCAIDHGARVWWFAE
                     DADGRGHWRFRSFGGGGDRRGLEGVADGSPRGLGLTPDGTVAVATAADGGTTVHIGPRG
                     GRARTVTRVEGHAVLAGISPDGRHLAVGGPAGSDRAVTVLSPDGTVRAVLGGGPHGGEL
                     WALGFRPSTGDDGTRTGDATGDGDATRTGDGTGDGGDGTELLLVRQHRDRYVLAVWRPG
                     AGPVTWEWCAFDTEITASWYPDGGAVLVRQDRHGRSLLHRADPRTRSVSAVPAPPGTLL
                     AAAARPGGDVHCLWTSASQPPRMLSTAGTPLPPLGTVDGSVPGDHRETWTPGPDGPVHS
                     LLALPEGAAPAPAVFLVHGGPADHDRDAYDGVVHSLVASGFAVVRANYRGSTGYGPRWR
                     RAYPAGVGLTQVQDLAAVRADLVRRGLVRADATALWGTSWGGYLALLAAGVDPALWHAA
                     VAVKPVADCAAAHRTATPALRALDERLFGGTPDTVPEAYARSSPVRYVPRVRAPLLVVA
                     AERDDKCPPEQVRDYLRALALAGVRHEAMWLDTGHDGYDGRHHVAVLRRAIGFLGRELC
                     GSGRPDSRAARRPTPDVPLRPERDPGPPPDAATGTGTTPAHRRGGHRAEGRRQQRPAGG
                     "
     aSDomain        complement(7818..8234)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DDQ41_RS28140_Abhydrolase_1.1"
                     /evalue="3.30E-10"
                     /label="DDQ41_RS28140_Abhydrolase_1.1"
                     /locus_tag="DDQ41_RS28140"
                     /protein_end="549"
                     /protein_start="410"
                     /score="31.9"
                     /tool="antismash"
                     /translation="PAVFLVHGGPADHDRDAYDGVVHSLVASGFAVVRANYRGSTGYGP
                     RWRRAYPAGVGLTQVQDLAAVRADLVRRGLVRADATALWGTSWGGYLALLAAGVDPALW
                     HAAVAVKPVADCAAAHRTATPALRALDERLFGGTP"
     gene            complement(9461..10984)
                     /locus_tag="DDQ41_RS28145"
                     /old_locus_tag="DDQ41_28145"
     CDS             complement(9461..10984)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129146.1"
                     /locus_tag="DDQ41_RS28145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_28145"
                     /product="MFS transporter"
                     /protein_id="WP_109296982.1"
                     /transl_table=11
                     /translation="MARASSSGGADGLGLRGAPGGHPRPAPDGTPDGPDRGARRRGTPR
                     DIGLLWGANTADALGTQMSGVAFPLLLLGLGHPPATVGLLAGAGVLVTLVCGPVAAVAA
                     DRGLRKPVMAGSAVVAAAAMGAVALSVAGGRPPLALLLGALLVERTATACFEAAARGTV
                     ALVAAPREVPRVVAGLEAGDRAALLAGPLLGGVLYQLARPLPFAADALSYVVTSLCVRS
                     MRSDLRAGSAAAAPSPDDTPGAAPLPAPDPAGPPGESPRRPAPPHPPGTPAPAPATPGA
                     PVPGGAADVPDAGPPERTPTPLSAPASHARDALAGLVLLRCAPRLRLILVWTTTVNAAL
                     AALYFAAIFALQDRGGPVAMGLVLAASGGAGLAGALAAPAVVRRVGGARTLVAVTWLTV
                     LPAAGLTVARSPWGLGACFGAFCLLLAPATVVVQSTVVADTPHHLQARTGALMAMAVVG
                     AGAGSPVVAGMLATYAGPAATPAVCALALAVLALFTGLRADRLRSGGRP"
     misc_feature    complement(10940..10942)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    11140..11153
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    complement(11466..11481)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
ORIGIN
        1 cgacgtgcgg ttccgccgca caccgcacac accgcccacc cccgaccaca ggaggcgcca
       61 gatgaccggg caggaccccg ccgccgtacg catcgacacc ggcaagccgc acccggcccg
      121 gatgtacgac tggttcctcg gcggcaagga caactacccg gtcgacgagg agatggcccg
      181 gcaactcctc gaactcgacg cccgcggccg cgacatggcc cgggtcaacc gggccttcat
      241 gcaccgcgcc acccgctggc tcggcggcaa cggcatccgc cagtacctcg acgtcggcac
      301 cggcatcccc accgagccca acctccacca gatagcccag caggtcgcgc ccgactcccg
      361 tgtcgtctac tgcgacaacg acccgatcgt cctcgcccac gcggaagccc tgctgcgcag
      421 cacccccgag ggcgccaccc ggtacatcca ggccgacgcg cgccgtcccg gcgccatcat
      481 cgaggcggcc ggcgaggtcc tcgacttcga ccggcccgtc gccctgtcgc tgctcgcgct
      541 gctgcacttc gtggacgacg aggacggcgc ctacgaactg gtgaaccggc tcgtcgggcg
      601 gctcgctccc ggcagctacc tggtgctgtc ccacaccacc ggcgacttcg accccgagaa
      661 ggccgccgtg gcccgggcga tgtacaagga gcggggcctc accctgcgcc cccggtcccg
      721 ggacgagttc acccggttct tcgacggcct cgacctcgtc gcgcccgggg tgtcgctgac
      781 cgccgagtgg caccccgggc tcggtgaggc cgtcgccgtc acgggtgacg agccgatccc
      841 cggctacgcg gccctcgcgc gcgtccgctg agggagtccg gccggccccc gcgcgcgtca
      901 gcgcccggcg tcgggcaccg ggctcccccc gggcccggtg tccgggtcct cgcccaccgc
      961 atccgcacgg ccgtccccac ccgctctgcc gccccctcga cgccccgtcc ggcgccgggg
     1021 tggtcccggc cgccggtctt ccccggtgcc cgcgcgctcg aatccggtac ccgcgcggtg
     1081 gcgggcccag tacccgtgcg gtggcgggcc cagcggctac ccggggcaag cggggccggc
     1141 acgtaccgcc ggcgcggtga gtgatcgccg cgttcccggc acggcatccc ggcctgcccg
     1201 taccggccgc ttgcgatctc cggtcggcgg gcgttacctg gcagtagacc gggctggagg
     1261 gtcccttgag ccggggtcgc gcggtcgcgt cccggccggt gccccgcctg cgggccctcg
     1321 ctcgacacca cgctgatccg tacggggcgg acagcggctc ggcgggccgc cacccggcgc
     1381 cgggcccgcc gccggaccgg ggaagacccg gggacgggag gtcgcagggt gcctggttcc
     1441 gcggccgggg gcagcgagcc caccgtggcg tctcacggtg ccggtgggcg ggcggtgcgt
     1501 gatcggcgca ccgacgagga ccggcgggcc gacgagcgca gggccgccga cgccgggctg
     1561 gacgcagcgc aggccgccgc acggcggcag cgctacgggc ccaacgtcct ggaagaggag
     1621 cgacgcagcg tcctgctgga gttcctgtcg catttctggg gcccgatccc gtggatgatc
     1681 gaggccgccc tggtgctgac ggcggtgacg cggcggtggc tggatttcgc gatcatcctg
     1741 gccctgctgc ttctcaacgg gctcgtgggc ttctgggagg aacaccaggc gcggggcgcg
     1801 atcgccgcgc tcaggaagcg cctggcgaaa gtggcgcagg tcaagcggga cggacagtgg
     1861 acgacgcttc cggcctccga actggttccg ggagacctcg tccgtgtcgt ccgagggcag
     1921 gtggtgccgg cggacgggac ggtggtcgag ggggagggcg aggtggacga gtcggcgctg
     1981 accggggagt ccctgccggt ggccaagaac cccggtgacg gcatgtactc cggatcggcg
     2041 gtgtcgcgcg gcgggccggt ggtgcgcgta ctggccacgg gcgcggacac ggagttcggc
     2101 cgtaccgctc agctgacggg gcgggcggcg ccggtgagcc acttccagcg cgcggtcgtc
     2161 aacatcggga agtacctgag tctgctcgcg ctcgcgctgg tcgccgtgat cgtcgtgctc
     2221 tccctgctgc gtggcagcgg ggtgacccgc acgatggagt tcgcactggt ggtggtcatc
     2281 gcctcgatcc cggtcgcgct gccggcggtg ctgtcggtga ccatggccgt cggagcccgg
     2341 tacctggccg gacgggaggc cgtcgtcagc catctgccgg cggtggagga gatggccggg
     2401 gtggacgtgc tgtgtgccga caagaccggc acgatcaccc gcaacgagct ggccgtcgcc
     2461 gacgtcgtcg tactccggga ggggacggac gaggggcggg tgctgctgca ggccgccctc
     2521 accgcggagc cgggcggcgg cgacccggtc gacgaggccg tcctggaggc gtgcggtgcc
     2581 gagcggctgg agggctggga ggtcaccgac ttcacaccgt tcgactcggc ccgcaagttc
     2641 gcccgggccg aggtgcgggg ccccggagcg gaacgcgccg tggcgaaggg ggcggtgcag
     2701 gccgtcctcg gcctcgccga ggccgacgcc tcggtcgcgc agcgcgtgga ggaggtgaca
     2761 cggggcttcg cgggccgggg tttccgcgcc atggccgtcg cctgcgccga cggcggcgag
     2821 tggcgggtga ccgggatgct gggactgcag gacccgcccc gggaggactc ccgtgcgacg
     2881 ctggaggaag ccgggcggct gggcgtgcag gtcaggatgg tcaccggcga ccgggtggag
     2941 atcgcccggg agatctgcgg ccgggtgggc atgggaaccg acgtgctgga cgccggacgg
     3001 atcgagcacc ttcagggcga cgacctcgct cgcgcggtcg agcgggccga cgggttcgcg
     3061 caggtggtac ccgaggacaa gtaccgcatc gtgaaggcgc tccaggaaca cggccacatc
     3121 gtcggcatga ccggtgacgg agtgaacgac gcccccgccc tgcagcgcgc ggacgcgggg
     3181 atcgcggtct tcggcgccac cgacgcggcc cgcgccgcct ccgacatcgt cttcctgtcc
     3241 ccgggactgt cgacgatcat cgaggccgtc caccggtccc gtgaggtgtt ccggcggatg
     3301 aagaactacg cgatctaccg gatcaccgag accatccggg tggtgctgtt cgtcaccgcc
     3361 accatcgtgg tgttcgcgtt cttcccggtc acaccgatcc aggtggtcct cctggccatc
     3421 ctcaacgacg cggccatcct gtccatcgcc tacgaccggg tacggccctc ctcacggccg
     3481 gagcgctggg acctgaccga ggtgatggcc gtcgccgccg tccttggcct gttcggcctg
     3541 gtctcgacgt tcgtcctggt gtgggtgaca catctgccgc tcggactcgc cgatgccgac
     3601 gtgcggaccc tgatctacct caagctgtcc gtcagcgggc acctcacggt cttcctggcc
     3661 cgcacccgcg gcccgttctg gtcctaccgg ccgtcctgga ttctgctcac ggccgtgatc
     3721 ggcacccaga tcctcgccac cgcgatcgcc ctgaccggat tcctgatgca tcccatcggc
     3781 tggagcctgg tcggactggt atggggttgg gccttcgccg agttcctgct gctggacccg
     3841 gtcaagctga tggcctaccg cgtcctggag cgccacggca aggcggcgaa agccgtcagc
     3901 tcctgatacc cctgcctgat acccccgctt gatcccactg cttggtctcc ctgcctgatc
     3961 ccctgcttgg tctccccgcc tggtccccct gccgggtcct cttgcctggt cccccctgcc
     4021 gggtcccccc cgtccgcggc gcctggcccg tgccggttcc cgcggcgacc tcgaagcaca
     4081 tcaggcgtcc cacgggttcg ccccggcgcc tcatcgtctc cgtccggacc acccgctccg
     4141 gggcctcgcg ggccgcccga actgcgaggc cccacgccga actcagacgg ccatcgcctc
     4201 ctggatccag cgcttcaccc ggtacggcag ccaccaggcc aggtcgccga cgcgcagcac
     4261 gagatgctcc tcggtgaagt cggccagggc gtccggtgcc acgtccgccg ccggggcgcc
     4321 ggggtccagc cgggcgatgg cctcgtggta gtgcggctcg tcgcaggtga agcggcgcag
     4381 gtcgccccag cggcggtcgc ggatctggag aaaccccgga ccctgccgcc acaggcactt
     4441 gcacaggtag tgggtgccgc gccacgcgat cagctgctcc tgggcgccct tcgcaccctc
     4501 cagccgctgc ggtggctgga gatggctcag cagcttccat gtctcgtcgt cgccggggcc
     4561 gacccgcagc cgccattcga cgagcacggc acgggcggtg aggtccctga ccaggcacag
     4621 tgaccggacc gtgcggcggg ccgcggcagg ggcggtgttg gcggcgggcg tcaggtcgac
     4681 ggtgcgggac agcgtgaccc gccgggcgcc gaggccccac agccgctcgg tctcctcgcc
     4741 catcggaccc gtcagccggt ggctgccgag cgccatgccc ggcagggcgc aggcgtccgg
     4801 gtcgtagtcg cgccaggcgg tgaccgtcag cgggatgccg ccggcggccg tgttcggggg
     4861 gtggactgcg ctggtcatgg tggtctcctg gagggtgcgg ttaccggggc tgcggtgtcg
     4921 gaccgtcacg gggcccgacg gtcaccggtg tcggaccgtc acggggcctg acggtcacgg
     4981 gtgtcggacc gtcacgggtg tcagaccgtc acgggcggct gggcggggcc gcggtcccgg
     5041 tccgccgggg tccgggcggc ggcgccgctc ccgccgccgc ccgcctcccg cggcgctccg
     5101 gcaccggcgg tgtgcagatg ccgcatgaag tccagccgca gcaggtcctc gttcaccgcg
     5161 gccggggcga cgtgcacgta ctgtcccccg tcggtgaaga cgatgccccg tgccacccac
     5221 tcgtcgagga cgcgctccac ctcctcccgg ccgcgcgggt cgccgggtac gcgggccgcg
     5281 gccttgcggg cgagcgcggc cggcgcgtgc ggctggtcca gcagccggaa cagcgcgatc
     5341 tcgaacggct cggtcagctc catggcacgc cacgggaacg cgcggcgccg gctgacgagc
     5401 acgatccggt caccgaggtc ggtgtgcgtc agccgggcgt cggcgtggtg cctcttccat
     5461 ccggccagcg cgtcgttgag ggcggtcacg gtcggttcgc cgatgccgcg ctccggggcc
     5521 tcgaagacat aggcgaggtc gtacagctcc tcctcgggca ggtcgtaggt gaagcggtag
     5581 tgcgcctcgg gccgcagccc cgtgaagccc agctcggggc ggttgaagta cgggctgaac
     5641 cgttcgatgg cgatgcgggc ggacaggtcg acgggcgggt cgagatgctc cagtgccggg
     5701 atctgcgcga tgaccggctc gtagtcgtcc gcggtctcac cggggaagcc gtgcaggtag
     5761 ttccaggaca ccgacaggcc cgtctccgcc ccgtcgcgca gcatccgcac gttctggcat
     5821 ccgctgacgc ccttgtccat caggtccagc acccggttgt tgaggctctc gataccgggc
     5881 tgcacgtaga tgagccccgc ctcggacagg gtgcgcagct gggaccgccg catgttcgcc
     5941 ttgatctcta tgtgcagccg caggtcgaag ccgctctcga tgatcctggg cagcacggtg
     6001 cccaggtagc ccatgtcgag gatgttgtcg acgacgtaca tgtccaggac ccgatggcgc
     6061 cgggccaggt ccatgatctc ctcgtagaag acttccgggc tcttgctgcg gaactgcatg
     6121 aacgaaccgt tgaggccgca gaacgtgcag tggtgcttct ccccccacca gcagccgcgg
     6181 gccccttcga cgacgagctt cggctccacc cagttgcggg ccaccgaccc ggccaggcgc
     6241 tcgaagtagc cgctgtagtc gggcgggagg atgtgggcgg gcggcagcgg cgccgtggcc
     6301 atcgggttga cggtgctcgt gccgtccggc gcccggtggc acaggcccgg gacggcgtcg
     6361 agaccggtcc gccccccggc cagggcccgt accagttccg ggaacgcggt ctccccctcg
     6421 ccgcgcacca cgtagtccac gaacgggaag ttgcggtgga ccgccgcgcc ctgctcggca
     6481 tcgcagttgg ccccgcccac gaccgtcgcg atgtgcggcg cgagccgctt gacgtggcgg
     6541 gcggccgcga gcgcggcggt gttctgctgg aaggtggagg tgaagccgac gacgtccggg
     6601 gcgagcccga cgatgcgccg ggcgatctcc tccacgaact gcggcacctc gcggtgcagt
     6661 tcgcgcgtca tccgcatccg tgatccgcgc agcttgccct tcatcaccga cgcgaactcc
     6721 tcctcccgcc aggaggggtc gtcgtagagc gcggaggaga acacccagtc gccgcacccc
     6781 atgaagtagg aggacaggga gtagtactgg tagtcgtccg cggtgaaccc tcggcgcgcc
     6841 gtgatccagt cggtgaactc caggttggca tgcagcacct cggccgcggc tcccgggacg
     6901 cgctcgtcga cactgcgctt gaggatgccg agggcgaggg agggcaggtc gatgggggac
     6961 cagggcatgt tgaccagcag tacgcgcaca tcggctcctt gacggtcctc gagcggtggg
     7021 tcggcggggc cccgacgggt ctcgccggcc ccgtgggggc tcttcgcggt ggtgtgtcgg
     7081 gtgcggtggt gtgtcgggtg ccgggggtgg gacggccggc cgggcggacc cctcgggccg
     7141 gtccgcccgg tggcggccgg ccgcgccggg cgcgccgggt gggcgccggc aagagccgac
     7201 tcgtcctccg gcccctgcgc gccgcgccgg gggcgccggg cgggcgccgg gctcgggggt
     7261 accggccggc ccgggtgtgc gccgggccgg ccgggccgga tcagtcctcc tcggagtcct
     7321 cggcgttgcg gaacgggatc gtgatggtga tcccgatgcc cggcttgcgg ttctcctcgt
     7381 cacccgccag cgggtcgttg ttgacgacgt ccttctgcac gatgtcctcc tctccggtgt
     7441 gctggggtgg tgcccgtccc ggtggccgcg tccggcggcg gtccgggatc ccgctccggc
     7501 cggagcggga cgtctggggt cggccggcgc gccgcccggg agtccgggcg gccggagccg
     7561 cacagctcgc ggcccaggaa accgatggcg cgccgcagca cggcgacgtg gtggcggccg
     7621 tcgtagccgt cgtgccccgt gtcgagccac atggcctcgt gccggacgcc tgccagtgcg
     7681 agcgcccgga ggtagtcgcg cacctgctcc ggcgggcact tgtcgtcgcg ttcggccgcg
     7741 acgaccagca gcggggccct cacccggggc acgtagcgga ccggggagct gcgggcgtac
     7801 gcctcgggca ccgtgtcggg cgtgccgccg aacagccgtt cgtcgagggc gcgcagcgcc
     7861 ggggtggcgg tgcggtgcgc ggccgcgcag tcggccaccg gtttcacggc gacggcggcg
     7921 tgccagaggg cggggtcgac acccgcggcg agcagcgcca gatagccgcc ccacgaggtg
     7981 ccccacagcg cggtggcgtc ggcccgcacc agaccgcggc ggaccaggtc ggcccgtacg
     8041 gcggcgagat cctgcacctg ggtcaggccg acgcccgcgg ggtaggcgcg gcgccagcgc
     8101 ggcccgtagc cggtggagcc ccggtagttg gcgcgcacga ccgcgaaacc ggaggccacc
     8161 agggagtgca cgacaccgtc gtaggcgtcg cggtcgtgat ccgcgggtcc gccgtgcacc
     8221 aggaacaccg ccggagccgg cgccgccccc tccggcaggg cgagcaggct gtgcaccgga
     8281 ccgtccgggc ccggggtcca tgtctcgcgg tggtcgccgg gtacggagcc gtcgacggtg
     8341 ccgagcggcg gcagcggggt gcccgcggtc gacagcatcc gcggcggttg cgaggccgac
     8401 gtccacaggc agtgcacgtc cccgccgggg cgcgcggccg cggccagcag ggtgccgggc
     8461 ggcgccggca cggcggagac cgagcgcgtg cgcgggtcgg cgcggtggag caggctgcgg
     8521 ccgtggcggt cctgccggac cagcacggcg ccgccgtcgg ggtaccagga ggcggtgatc
     8581 tccgtgtcga acgcgcacca ctcccaggtg accggtcccg ctccggggcg ccagacggcg
     8641 aggacatagc ggtcccggtg ctgccggacg aggagcagtt cggtgccgtc gccgccgtcc
     8701 ccggtgccgt cgccggtccg ggtggcgtcg ccgtccccgg tggcgtcgcc ggtccgggtg
     8761 ccgtcgtcgc cggtcgacgg ccggaagccg agagcccaca gttccccgcc gtgcgggccg
     8821 ccgcccagta ccgcgcgtac ggtgccgtcg ggggacagca cggtcaccgc gcggtcggag
     8881 cccgccggtc cgccgacggc cagatgccgc ccgtcgggtg agatccccgc gagcacggcg
     8941 tgaccctcga cgcgggtcac ggtcctggcc cggccgccgc gcgggccgat gtggacggtg
     9001 gtgccgccgt cggcggccgt ggcgacggcg accgtgccgt cgggggtcag accgaggccc
     9061 cggggggagc cgtccgcgac cccctccagg ccgcgccggt ccccgccgcc gccgaaggac
     9121 cggaaccgcc agtggccgcg gccgtcggcg tcctcggcga accaccacac ccgggcgccg
     9181 tggtcgatcg cgcagcgcag cgtgccgccc ggacggtccg tcacctgctc ggcccggccg
     9241 gtcgtcgcgt cccaggtgaa cacctggcag cgtccgtcgg cgtcggcggt gaacaccatc
     9301 cgcgccgggt cggcggcgca gacgtccggc agttcggcgc ggtaggtccg gaagcgggga
     9361 ccgggggcct cgccgccgcc gccgtcggcg gcggcccggg gggccgcgga cgccggggcg
     9421 cggacggggc ggtgcgggcc gttcccggtg ggcgggcggg tcacggccgg cctccggagc
     9481 gcagacggtc ggcccggagg ccggtgaaca gggcgaggac ggcgagggcc agggcgcaga
     9541 ccgccggggt cgccgcgggg ccggcgtagg tcgcgagcat cccggcgacc accggcgaac
     9601 cggccccggc ccccacgacc gccatggcca tcagcgcgcc ggtcctcgcc tggaggtggt
     9661 gcggggtgtc ggcgacgacg gtggactgga cgaccaccgt ggccggtgcg agcagcaggc
     9721 agaacgcccc gaagcaggcc ccgaggcccc agggcgacct tgccacggtg aggccggcgg
     9781 cggggagcac cgtcagccag gtcacggcca ccagcgtccg ggcgccgccc acccgccgta
     9841 cgacggcggg cgcggcgagg gcgccggcca gcccggcgcc gccggacgcc gccagcacca
     9901 gccccatcgc gaccgggccg ccgcggtcct ggagcgcgaa gatcgcggcg aagtagaggg
     9961 cggcgagtgc cgcgttgacc gtggtggtcc agaccaggat gagacgcagg cggggcgcgc
    10021 aacggagcag tacgaggccg gcgagggcat cgcgggcgtg cgaggccggc gcgctcagcg
    10081 gagtgggcgt ccgctcgggg ggtccggcgt ccgggacgtc cgccgcaccg ccgggcacgg
    10141 gggcgccggg cgtcgcggga gcgggggccg gggtgccggg cgggtgcggc ggtgccggcc
    10201 gccgcgggga ttcgccgggc gggcccgccg gatccggtgc gggcaggggc gcggcccccg
    10261 gcgtgtcgtc cggtgagggg gcggccgcgg ccgagccggc ccggaggtcg gagcgcatgg
    10321 accggacgca cagggatgtc accacgtacg acagcgcgtc ggccgcgaag ggcaggggcc
    10381 gggcgagctg gtacagcacc ccgccgagga gcggaccggc caggagcgcg gcgcggtcgc
    10441 cggcctccag cccggccacg acgcggggca cctcccgggg cgcggcgacg agcgcgacgg
    10501 tgccgcgggc ggcggcctcg aagcacgcgg tggccgtgcg ttcgacgagc agcgcaccga
    10561 ggagcagcgc gagcggggga cggccgcccg ccaccgacag ggccaccgcg cccatggccg
    10621 ccgcggccac gacggcggag ccggccatca cgggtttgcg tagtccccgg tccgccgcca
    10681 ccgccgcgac cggcccgcac accagggtga cgagcacgcc cgcgccggcg aggagcccga
    10741 cggtcgccgg cgggtggccg agtccgagga ggagcagcgg gaaggcgacg ccggacatct
    10801 gggtgccgag ggcgtcggcg gtgttggcgc cccacagcag tccgatgtca cgcggggtcc
    10861 cgcggcggcg ggcaccgcgg tcggggccgt cgggtgtgcc gtccggggcc gggcgggggt
    10921 gtccgcccgg cgcgccccgt aagccgaggc cgtcggcgcc gccggaagac gaagcacgtg
    10981 ccatgccctg cctcccccgg gccgcggtgg cacctcccgc cgtcacgtca ccggtacctc
    11041 cgttctcgcg gagagcacgc agatgtgcgg gaaatgtgcg ggaagtgaaa tcgagcggag
    11101 cctagccacg gcgattcggg caccgcaagc agtcggacgt cgaccgaatc tgacgcaaat
    11161 cgatcgtcgg ccatcgctgt acgagggcgg gcggcatcat cgcgccttac ctgtccggtt
    11221 ctggcccgcg accggccgcg gaacgcaccg cgccccccgg aaccgcggct tccgcgcctc
    11281 cgcttcgcat cggcgcacct ctcttcccct cctgcccctg aacgggtgga acctgccgcc
    11341 tccggtgcac ctgtgcgcgt tccgctccgt gcgcggtggg ccggggccca gtcgtcacgc
    11401 tgggggtgtt tgtccgcgca aagcgctccg ggcgtgcgct ccgaacggtg ctccgcgtgg
    11461 ccggtgtgac ggatgagtca catcggtctc ggctcgcgcg ccgccgggcg gcgcaccggc
    11521 cgcgctctcc gaatggcgcg aaagcgcccc tcccgtcggc gccgggagcc cggcgcggaa
    11581 cgcgttcacc ccgtccggcg ggataccggc gtgcgggccg cacccagggg gaagccgcgc
    11641 acgaaaccgg tcgccccgcg ccggtcgccc cacgccggtc cgccgccggc ggccgggtcc
    11701 gtccacgcct ctgcgcctcc ccggtaccct gccggcgtct ccccggcatc ttgccggtgc
    11761 cttcgaagtg ccccggtgcc ttcgcggtgc ccccggtgcc ttgccggcgc tttcccggta
    11821 cctccccggt acctccccgg taccttcgcg gacgagcggc gtcggtccgc cgggaactgc
    11881 cctcggcccg cttcggccgc gcgccgcttc ggccgccgcc gcgccgcctc cgtgcccggc
    11941 cgcggcgtca gccgcctccc cgccgcagac gtaccgtcac gatctggaa
//
