LOCUS       NZ_CP029254            11611 bp    DNA     linear   CON 23-OCT-2024
DEFINITION  Streptomyces spongiicola strain HNM0071 chromosome, complete genome.
ACCESSION   NZ_CP029254
VERSION     NZ_CP029254.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces spongiicola
  ORGANISM  Streptomyces spongiicola
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7180417)
  AUTHORS   Zhou,S. and Huang,X.
  TITLE     Complete genome sequence of the Type Strain of Streptomyces
            spongiicola HNM0071, the producer of staurosporine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7180417)
  AUTHORS   Zhou,S. and Huang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2018) Institute of Tropical Agriculture and
            Forestry, Hainan University, No 58, Haikou, Hainan 570228, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 850.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003122365.1-RS_2024_10_23
            Annotation Date                   :: 10/23/2024 01:04:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,921
            Genes (coding)                    :: 5,739
            CDSs (with protein)               :: 5,739
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 47 of 182
            Pseudo Genes (incomplete)         :: 161 of 182
            Pseudo Genes (internal stop)      :: 9 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:10:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5988096
            Orig. end                         :: 5999707
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029254.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11611
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="21"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11611
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11611
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6611](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6611)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(280..295)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.0"
                     /tool="antismash"
     gene            625..2070
                     /gene="hemG"
                     /locus_tag="DDQ41_RS26110"
                     /old_locus_tag="DDQ41_26110"
     CDS             625..2070
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888540.1"
                     /locus_tag="DDQ41_RS26110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_26110"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_109296654.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 4.4e-07, bitscore:
                     27.9, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="DAO (E-value: 1.3e-09, bitscore: 36.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLPTSEDPRARAGRVVVIGGGIAGLAAAHRLATSGVRVTLLEAGG
                     RLGGKLRTGEIAGAPVDLGAESVLARRPEAVALARAVGLGDRLRPPATTTASVWTRDAL
                     HPMPRGHVMGVPGTPEALSGLLSAEGVARIGRERELPPAELAEDVAVGTFVAERLGHEV
                     VDRLVEPLLGGVYAGDAYRISMRAAVPALFDAARAAGGNLLDGVREVQRRTAERHETGP
                     VFMGLDGGVGTLPAAVADAVRAAGGEILTETPVLRLTRGADGWRIRTDAAELIADGVVL
                     AVPAWSASVLLADAAPAASAELSRVEYASMALVTLAFRAADTAVLPAGSGFLVPPVDGR
                     TIKASTFSAQKWGWVADRAPGLFLLRTSVGRYGEEKQLDREDDDLVAASLGDLAEATGL
                     TARPVASRVTRWTDGLPQYPVGHLGRVARVRDAVAALPGLRVCGAAYDGVGIPACVGSG
                     QRAADEVIATAPLVRGAVREGRE"
     gene            2075..2794
                     /gene="hemQ"
                     /locus_tag="DDQ41_RS26115"
                     /old_locus_tag="DDQ41_26115"
     CDS             2075..2794
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962204.1"
                     /locus_tag="DDQ41_RS26115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_26115"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_109296655.1"
                     /transl_table=11
                     /translation="MTAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPQDADRAGYA
                     GEVQELFDRLAAEDVTVRGTYDLSGLRADADLMVWWHAETADRLQDAYNLFRRTRLGRA
                     LEPVWSNMALHRPAEFNKSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRRMLAD
                     HGRMARGYPDVRANTVASFSLGDYEWLLAFEADELYRIVDLMRHLRASEARMHVREEVP
                     FYTGRRKSVADLVAGLA"
     gene            complement(2865..4460)
                     /locus_tag="DDQ41_RS26120"
                     /old_locus_tag="DDQ41_26120"
     CDS             complement(2865..4460)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (101-475). E-value:
                     3.8e-23. Score: 74.3. Matches aSDomain:
                     nrpspksdomains_DDQ41_RS26120_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888542.1"
                     /locus_tag="DDQ41_RS26120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_26120"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_109296656.1"
                     /sec_met_domain="PF00561 (E-value: 2.6e-26, bitscore: 91.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKAALYGAAGSLILSALAAAPAAGTPRSGGPEDRGTAVAAARAV
                     AAGVTFGACPAEEDLPPTVECAAVRVPLDYADPWGRQISIAVSRVRAAGGPSVRQGALL
                     YNPGGPGGSGMYFPALADEPEWKRIAEAYDLVGYAPRGVGRSAPISCQHPAEFTKAPTS
                     APVHPSAAEKRQGVARARAYAQGCARNAGPALRHYHTLNNVRDLEVLREALGERRLTFL
                     GASYGTYLGALYAMTFPDRVRRMVLDSPVDPDPAKIWYRNNLEQSLAFESRWADFRAWV
                     ARHHATYGLGTTPRQVQAAYDRARALLAENPADGAVGPGQLQAAFTRTVYNDLYWPRRA
                     LALSAYLKGDPRQLVKQAKPRASAAAEEENGNAVYTAVQCNDAPWPTDWAVWDRDNTAL
                     ARRAPFETWANVWMNLPCAYWQQPRQRPVDVRTGPGDVPPLLILAAERDAATPYAGALR
                     LQERLAGSVLVTERDAGTHGISGGDNACVNGHMEAYLLTGRTPVRRASCAPHPQPDPVS
                     LERRAVERVLPPAV"
     aSDomain        complement(3036..4157)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DDQ41_RS26120_Abhydrolase_1.1"
                     /evalue="3.80E-23"
                     /label="DDQ41_RS26120_Abhydrolase_1.1"
                     /locus_tag="DDQ41_RS26120"
                     /protein_end="475"
                     /protein_start="101"
                     /score="74.3"
                     /tool="antismash"
                     /translation="ALLYNPGGPGGSGMYFPALADEPEWKRIAEAYDLVGYAPRGVGRS
                     APISCQHPAEFTKAPTSAPVHPSAAEKRQGVARARAYAQGCARNAGPALRHYHTLNNVR
                     DLEVLREALGERRLTFLGASYGTYLGALYAMTFPDRVRRMVLDSPVDPDPAKIWYRNNL
                     EQSLAFESRWADFRAWVARHHATYGLGTTPRQVQAAYDRARALLAENPADGAVGPGQLQ
                     AAFTRTVYNDLYWPRRALALSAYLKGDPRQLVKQAKPRASAAAEEENGNAVYTAVQCND
                     APWPTDWAVWDRDNTALARRAPFETWANVWMNLPCAYWQQPRQRPVDVRTGPGDVPPLL
                     ILAAERDAATPYAGALRLQERLAGSVLVTERDAG"
     misc_feature    complement(4512..4525)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.06"
                     /tool="antismash"
     gene            complement(5001..6611)
                     /locus_tag="DDQ41_RS26125"
                     /old_locus_tag="DDQ41_26125"
     CDS             complement(5001..6611)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037016.1"
                     /locus_tag="DDQ41_RS26125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_26125"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_109296657.1"
                     /sec_met_domain="DUF692 (E-value: 1.2e-91, bitscore: 305.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKHLDRLGTGIGWRPEIAAAVERLPGLDWVEVVAENVCPGHLPDS
                     LRRLRERGVTVVPHGVSLGLGGAGRPDPRRLADLAERAEALGAPLVTEHIAFVRAGGAL
                     TASPPLEAGHLLPVPRTRDALDVLCENVRIAQDGLPVPLALENIAALISWPGEEMTEGR
                     FLAELVERTGVRLLVDVANLHTNRVNRAEDPLRALDELPVEAIAYVHVAGGVERDGVWH
                     DSHAHPVPRPVLDLLAELCSRTDPPGVLLERDDAFPPGEELAAELDVIRAVLDSPPRRA
                     PGTRAADGPAPGALAGLPDGARGAAGRDTADTAHPAGRANPADDAHRADDADTTHDAYP
                     SDDVDTTDTTDATDATDATDATDAADAADGCSATGAADGCSAAHEPVSPAGATAQCRAP
                     DDIARERLGLAQAALLSALVAGTPAPEGFDARRLRVQSRALAAKRADVVARVAPELPQI
                     LGTGYRPAYLGYATSRPMRGGYRRDALDFAEQLLAAGRPEDPVARRRLAQWWRDRAGPR
                     PPRRATRFVRAARAALVRR"
     gene            complement(6804..7583)
                     /locus_tag="DDQ41_RS26130"
                     /old_locus_tag="DDQ41_26130"
     CDS             complement(6804..7583)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018847910.1"
                     /locus_tag="DDQ41_RS26130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_26130"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_172607569.1"
                     /transl_table=11
                     /translation="MRRVNGTALIIAALVATVGALAFPVWSYADRSGTGQANLNASSVG
                     TQWGPLTATDRDFLVKVRLAGLWELPAGQQALERAPTQAIKDAGDHLIVGHTDLDQRAR
                     DVAAKLGVELPNVPTEQQQGWLRELSAASGREYEYKFANLLRNAHGKVFALIAQVRHST
                     RNTLIRQLASDANQTVLDHITMLENTGMVDFDAIANEAAGAATASPTGPPPPDGNLPPA
                     PTPAVPPGGIETTSEPSTQPGPPTAVNTNRPAPGASS"
     misc_feature    7902..7920
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.16"
                     /tool="antismash"
     gene            complement(8060..8743)
                     /locus_tag="DDQ41_RS26135"
                     /old_locus_tag="DDQ41_26135"
     CDS             complement(8060..8743)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888545.1"
                     /locus_tag="DDQ41_RS26135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_26135"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_394342194.1"
                     /transl_table=11
                     /translation="MRQEPVPRDEAPQFVLPLVVRIERNEPPSRTDALETAARAVVLML
                     SDARSSGDGEWAARVRDWQDGRIRKVVRRARGAEWRKASELPGITAAGGGAEVRVFPPV
                     PLDGWPRELSKLQVSGTELEDRGQPVGDAGPGCALLWLNPGLHMSTGKEMAQVGHGAQL
                     LWWDMDEAGRTAWRDAGFPLAVRTAAAGRWAELAARGLPTVRDAGFTEVAPGSVTVVAE
                     HPVRH"
     misc_feature    complement(8911..8924)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.24"
                     /tool="antismash"
     misc_feature    complement(8916..8925)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            9101..10162
                     /locus_tag="DDQ41_RS26140"
                     /old_locus_tag="DDQ41_26140"
     CDS             9101..10162
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888546.1"
                     /locus_tag="DDQ41_RS26140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_26140"
                     /product="S1 family peptidase"
                     /protein_id="WP_109296660.1"
                     /transl_table=11
                     /translation="MGIAAAAVLLPNANASQSGSEDERTAPRKLSAASAADLLQQLGRQ
                     LGESYGGAYYDASKQQLIVNIVGDDNDVEIEVKRAGAVARSVQNSFSTLKSATRTLSSE
                     ATVPGTAWAIDPRNNQILVTADRTVTGERWNTVESAVESLGDGVARIQKSKGEFKPLLE
                     GGDAIFSNGGARCSLGFNVTTQDGQSGFLTAGHCTAASEQWSEQQGGQPVATAQESVFP
                     GEGDFALVTYDDPNTEAPSAVDLGDGRALEIGQAADAAVGQEVFRMGSTTGLNGGQVTG
                     LNATVNYAEGTVTGLIQTNVCAEPGDSGGALFTRDGNAIGLTSGGSGDCTSGGETFFQP
                     VTTALEAVGAQIG"
     misc_feature    9134..9152
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 24.95"
                     /tool="antismash"
     misc_feature    complement(10987..10999)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.13"
                     /tool="antismash"
ORIGIN
        1 acctccacgg ccagctcggc ggtcctgatg acatgtgccg cgggcaggcg ctgcgtccca
       61 ccgtcgtcgg gcgcgccgtc gtcgggcgcg ccggctctcg cgccggcggc tccggccgag
      121 gcgtcggcgg cggatccggg ggcggcggcc gtcgcggcct cgcctccggc ggcccggtcg
      181 gcggtcgagg cggatccgtc gtcggaggcg ccgcagccgg cgagtgagag tgagacgacg
      241 agtagcgccg ccgccggcct gtgccgtgca cgcatggtgg tccccccagg ggaatgcgat
      301 gggtttcggt gccggtttcg acgtgcggcc gccgaacggg gttgccccgt caggttccga
      361 ggcggtcacg gtagggactc gattggggac gccgagccac cgtgcgctgc gccgccgacc
      421 gcggaccacg gaccgccgac cgccgaccgc ggaccacgga ccgccgaccg cggaccacgg
      481 accgcgccgc ccggtcagcg cggcccccac cggcccgtgc acgcgagacc agcccgtgca
      541 cgggagaccg gcccctgcac gggcaccggc cgccgagccc gagccgatgc cggtccgccc
      601 ggggcggttt gagagagtgg aggcatgctg cccacgtcag aggacccccg ggcccgcgca
      661 ggccgtgtcg tcgtcatcgg cggtggtatc gccggcctcg cggcggcgca ccggctggcc
      721 acgtccggag tgcgggtgac cctgctggag gccggcggcc ggctcggagg caagctccgg
      781 accggggaga tcgccggagc ccccgtcgac ctcggcgccg agtcggtgct cgcccggcgg
      841 cccgaggcgg tcgccctcgc acgcgccgtc gggctcggcg accggctgcg gcccccggcc
      901 accaccaccg cctccgtctg gacccgcgac gccctgcacc ccatgccccg gggccatgtg
      961 atgggtgtcc ccggcacccc ggaggcactg tccgggctgc tgtccgccga gggcgtcgcc
     1021 cggatcgggc gggagcgcga actgcccccg gctgagctgg cggaggacgt cgccgtcggc
     1081 acgttcgtgg ccgagcgcct cggtcacgag gtcgtcgacc ggctcgtcga gccgctgctc
     1141 ggcggcgtgt acgcgggcga cgcctaccgc atctccatgc gcgccgccgt ccccgccctc
     1201 ttcgacgctg cccgggccgc cggaggcaac ctgctggacg gcgtccgcga ggtgcagcgc
     1261 cggaccgccg agcggcacga gaccggcccc gtgttcatgg gcctcgacgg cggggtcggc
     1321 accctcccgg cggcggtcgc cgacgccgtc cgggccgccg gcggcgagat cctcaccgag
     1381 accccggtgc tccgtctgac ccgcggcgcc gacggctggc ggatccgcac cgacgcggcg
     1441 gaactcatcg cggacggtgt cgtcctcgcg gtccccgcct ggtctgcctc cgtgctcctc
     1501 gccgacgcgg caccggcggc gtccgccgag ctctcccggg tcgagtacgc gtcgatggcc
     1561 ctcgtcacgc tcgccttccg cgccgccgac acggccgtgc tgcccgccgg gtccggcttc
     1621 ctcgtacccc cggtggacgg gcgaacgatc aaggcgtcca cgttctccgc gcagaagtgg
     1681 ggctgggtcg ccgaccgcgc ccccgggctc ttcctgctgc gcacctccgt cggccggtac
     1741 ggcgaggaga agcagctcga ccgggaggac gacgacctcg tggccgcctc gctcggggac
     1801 ctcgccgagg cgaccgggct caccgcccgc cccgtcgcct cccgcgtcac ccgctggacc
     1861 gacgggctgc cgcagtaccc cgtcggccac ctgggccggg tcgcccgcgt ccgggacgcc
     1921 gtggccgcgc tgcccgggct gcgggtctgc ggggcggcgt acgacggcgt cggcatcccc
     1981 gcctgcgtcg gcagcgggca gcgcgcggcg gacgaggtca tcgccacggc ccccctggtg
     2041 cggggcgccg tccgcgaagg gcgagaatag ccccatgact gcgcccgaga agatcccgaa
     2101 cgccggtaag aaggcgaagg acctcaacga ggtcatccgc tacaccctgt ggtccgtgtt
     2161 caagctgcgg gacgtgctgc cgcaggacgc ggaccgcgcc gggtacgcag gcgaggtcca
     2221 ggagctgttc gaccggctcg cggccgagga cgtcaccgtt cgcggtacgt acgacctgtc
     2281 cggtctgcgc gccgacgccg acctgatggt ctggtggcac gccgagaccg cggaccggct
     2341 ccaggacgcg tacaacctct tccgccgcac ccgcctcggc cgcgcgctgg agccggtctg
     2401 gtcgaacatg gcgctgcacc gccccgccga gttcaacaag tcccacatcc ccgccttcct
     2461 cgccgacgag accccgcgcg actacgtctc ggtgtacccg ttcgtacgct cctacgactg
     2521 gtatctgctg cccgacgagg accgccgccg gatgctggcc gaccacggca ggatggcccg
     2581 cggctacccg gacgtccgcg ccaacaccgt cgcctccttc tccctcggcg actacgagtg
     2641 gctgctcgcc ttcgaggccg acgagctgta ccggatcgtc gacctcatgc ggcacctgcg
     2701 cgcgtccgag gcgcggatgc acgtccgcga ggaggtcccc ttctacaccg ggcgccgcaa
     2761 gagcgtcgcc gacctggtgg ccggtctggc ctgacggccg agaaccaccc ggaccaggag
     2821 gcgggacccc cgcggggcgg cccgcctccc cccgacccgg aagatcagac ggcgggcggc
     2881 agcacccgct cgaccgcccg ccgctccagc gacaccgggt ccggctgcgg gtgcggcgcg
     2941 cacgacgcgc gccgcaccgg cgtcctgccg gtcagcaggt acgcctccat atggccgttg
     3001 acgcaggcgt tgtcgccgcc cgagatgccg tgcgtccccg catcccgctc cgtgaccagc
     3061 accgaacccg ccaggcgctc ctgcagtctc agggcgccgg cgtacggggt ggccgcgtcc
     3121 cgctccgccg ccaggatcag cagcggcggc acgtcccccg gcccggtgcg cacgtcgacc
     3181 ggccgctgcc gcggctgctg ccagtacgcg cacggcaggt tcatccagac gttcgcccag
     3241 gtctcgaacg gcgcccgccg cgccagcgcg gtgttgtccc ggtcccacac ggcccagtcc
     3301 gtgggccagg gagcgtcgtt gcactgcacg gccgtgtaga cggcgttgcc gttctcctcc
     3361 tcggcggccg ccgacgcgcg gggcttcgcc tgcttgacga gctgccgcgg atcgcccttc
     3421 agatacgccg acagggccag ggcgcggcgc ggccagtaca ggtcgttgta gacggtccgg
     3481 gtgaacgccg cctggagctg gcccgggccg accgcgccgt ccgccgggtt ctccgccagc
     3541 agggcgcgcg cccggtcgta ggccgcctgc acctgccggg gcgtcgtgcc gagaccgtag
     3601 gtggcgtggt gccgggcgac ccaggcgcgg aagtccgccc accggctctc gaacgcgagc
     3661 gactgctcca ggttgttgcg gtaccagatc ttcgccgggt ccgggtcgac gggggagtcg
     3721 agcaccatcc ggcgcacccg gtcggggaac gtcatcgcgt acagggcgcc cagataggtg
     3781 ccgtaggagg cgccgaggaa ggtcagccgc cgttcgccga gggcctccct cagcacctcc
     3841 agatcgcgga cgttgttgag cgtgtggtag tgccgcaggg ccgggcccgc gttgcgggcg
     3901 cagccctgcg cgtacgcccg ggcacgcgcc acgccctgcc gcttctcggc ggcggaggga
     3961 tggacggggg ccgaggtcgg cgccttggtg aactccgccg ggtgctgaca ggagatgggc
     4021 gcggagcggc ccacgccgcg cggcgcgtag cccaccaggt cgtacgcctc cgcgatgcgc
     4081 ttccactcgg gttcgtcggc gagtgcgggg aagtacatgc cggagccgcc gggcccaccg
     4141 gggttgtaga ggagcgcgcc ctggcgcacg gacggcccgc ccgccgcccg cacccggctg
     4201 acggcgatac tgatctgccg cccccacggg tcggcgtagt cgaggggcac ccggaccgcg
     4261 gcgcactcga cggtcggcgg caggtcctcc tccgccgggc aggcgccgaa ggtgactccg
     4321 gcggccacgg cacgggcggc ggcgacggcg gtaccgcggt cctccgggcc gccggaccgg
     4381 ggcgtcccgg ccgccggggc cgccgcgagg gccgagagga tcagggagcc ggcggctccg
     4441 tacagggctg cttttctcat cgcgttccct tcgcaccacc ggtcgtggct cgaaggtcac
     4501 cgggggtccg ggttttgtaa aggatcttgc tcgggtgtcg cggtcgatga cccttttgct
     4561 gcacgtgcga tgtcgcgcat gtgctgctgt gcaggtggcg tcgcgcatgt gctgttgcgt
     4621 tcgtgccgct gtgttgtgct gtgttgtgct gatctgtgtt gtgcttgtgc cgtcgtgtct
     4681 gtgccgttgt gtgtggtgtt gtgttcgtgg cgtcgtgtat gcggcgttga gggcgggcgc
     4741 ttgggcgcgg tgtcccgggc gggatgtgtc atcttcgcgt ggccgaaggc tcgttgccgg
     4801 ggatgctcgt tgccggtcgg cccgccgcag ggccgtcgga ttggggccgt ccgcttggac
     4861 ggccgtccgc tccgaggcgg tccgtcgccg gttccgaggg cggtccgtcg ccggtcgcgt
     4921 caccccgggg cggctgtcgc gggccgccct cgtggacgcg gcctccggca ggtggtgcgc
     4981 cgggccggcc cggcccgggc tcacctcctg acgagggcgg ctcgggctgc gcggacgaag
     5041 cgcgtcgcgc ggcgcggtgg acggggcccc gcccggtccc tccaccactg ggcgagccgg
     5101 cggcgggcga ccgggtcctc gggccgcccc gcggcgagca actgctcggc gaagtccagc
     5161 gcgtcccgcc ggtagccgcc gcgcatcggg cgggacgtgg cgtagccgag gtacgcgggc
     5221 cggtagccgg tgccgaggat ctgcggcagt tctggcgcca ccctggcgac gacgtcggcc
     5281 cgcttggcgg cgagcgcccg gctctgcacc ctcagccgcc gggcgtcgaa cccctccggc
     5341 gccggcgtcc cggcgacgag cgcggacagc agggcggcct gtgcgagccc gaggcgctcc
     5401 cgcgcgatgt cgtccggagc gcggcactgc gccgtcgcgc cggccgggga caccggctcg
     5461 tgcgccgcgc tgcacccgtc cgccgcgcct gtcgcgctgc acccgtccgc cgcgtccgcc
     5521 gcgtccgtcg cgtccgtcgc gtccgtcgcg tccgtcgcgt ctgtcgtgtc cgtcgtgtcc
     5581 acgtcgtccg acgggtacgc gtcgtgcgtc gtgtccgcgt cgtccgctcg gtgcgcgtcg
     5641 tccgccgggt ttgctctgcc tgccgggtgc gccgtgtccg ccgtgtcccg cccggccgcc
     5701 ccgcgggcac cgtccgggag ccccgcgagg gccccgggcg ccggcccgtc cgccgcccgg
     5761 gtgcccggtg cgcgtcgtgg gggcgagtcg aggaccgcgc ggatcacgtc cagttccgcg
     5821 gccagctcct ccccgggagg gaaggcgtcg tcccgctcca gcagcacccc tggcgggtcg
     5881 gtgcgcgagc acagttcggc gaggaggtcg aggaccggcc gcggcaccgg gtgggcgtgg
     5941 ctgtcgtgcc agacgccgtc gcgctccact ccgccggcga catggacgta cgcgatcgcc
     6001 tcgacgggca gctcgtccag ggcccggagc gggtcctcgg cccggttgac gcggttggtg
     6061 tgcagattgg cgacgtcgac cagcagccgc accccggtgc gttcgacgag ctccgccagg
     6121 aaccggccct ccgtcatctc ctcgccgggc caggagatca gggcggcgat gttctccagg
     6181 gcgagcggca cgggcaggcc gtcctgtgcg atgcggacgt tctcgcagag gacgtcgagc
     6241 gcgtcccggg tgcgcgggac ggggagcagg tgcccggcct cgagcggggg cgaggcggtc
     6301 agcgcgccgc cggcgcggac gaacgcgatg tgctcggtca cgagcggcgc ccccagtgcc
     6361 tcggcgcgtt cggccagatc ggccagccgg cgcgggtccg gccgtcccgc cccgccgagt
     6421 cccagggaga caccgtgcgg gacgaccgtg acgccgcgct ccctcaaccg ccgtagcgag
     6481 tcggggagat ggccggggca gacgttctcc gcgacgacct cgacccagtc gagaccgggc
     6541 agccgctcca cggcggcggc gatctccggg cgccagccga taccggtgcc gagcctgtcg
     6601 aggtgcttca tgggtccccc tcctccgtgt ctggaggggt catggccccg acgggccatg
     6661 accaatcccg gggagcggac gttcagagct tcatttgagg ttgcgcacac cccggtcggg
     6721 tgccgggccc gttgaaagcg cgggcccggc gcggtggtgg cgggtgcgcc gggagcctcc
     6781 cggcgcgggc ccgccacgcg tcgtcagctg ctcgcgcccg gtgccggccg gttggtgttg
     6841 acggcggtcg gcggccccgg ctgggtcgag ggttccgagg tggtctcgat cccgccgggc
     6901 ggcacggccg gggtgggcgc cgggggcagg ttgccgtccg gcggcggcgg ccccgtgggg
     6961 ctggccgtcg cagcgcccgc ggcctcgttg gcgatcgcgt cgaagtccac catgccggtg
     7021 ttctcgagca tggtgatgtg gtccagcacg gtctggttcg cgtcggacgc gagctgacgg
     7081 atcagggtgt tgcgggtgct gtggcggacc tgggcgatga gggcgaacac cttgccgtgg
     7141 gcgttgcgca gcaggtttgc gaacttgtac tcgtactcgc ggccgctggc cgccgacagc
     7201 tccctcagcc agccctgctg ctgctcggtg ggcacgttcg gcagctccac gcccagcttc
     7261 gccgccacgt cgcgggcacg ctggtcgagg tcggtgtgac cgacgatgag atggtcgccc
     7321 gcatccttga tcgcctgggt cggggcacgc tcgagtgcct gctgaccggc gggcagctcc
     7381 cacagcccgg cgagccgcac cttgaccagg aagtcgcggt cggtcgccgt cagcggcccc
     7441 cactgggttc ccacggacga ggcgttgaga ttggcctggc cagtcccgga ccggtcggcg
     7501 taggaccata cggggaaagc gagcgcgccc actgtggcga ccagtgccgc gatgatgagg
     7561 gctgtcccgt tgacgcgacg cagcaagtgt gcctcccgat ccgatgtgcg taccgcggaa
     7621 ccccggctag gtcaccggcg gttcgaactg ctgggcagta ccgggaggta cgtacggggg
     7681 agcggtcccg ttcagcccgg ggccggaaga tctccgcgcg gcggggtgag gaggcccgcg
     7741 tgccgcccgg gagggtctcg gtagcgcccc gggagggtct cggtaccgcc gggacggtct
     7801 ccgctccgca agcgaagatc tcgcgtgcgg ggcgggagcg cgcccgtacc gggtgggatc
     7861 acccggacgg atcccctgta agggagccgc tgtgcgggag ccgctgtaca gaacccccgg
     7921 tacagaacct cccccgtaca gaaccgcccg tacagccccc cggtacagaa ccccccggca
     7981 cagaaccccc gtcacggcgc gggagagtgc cctaccgctc gggaggagag gaggagcgcc
     8041 cgtgccgtcc ggcagggcgt cagtgccgca ccgggtgctc ggcgacgacc gtcacggagc
     8101 ccggggcgac ctccgtgaaa cccgcgtccc ggaccgtcgg caggccgcgc gcggcgagtt
     8161 cggcccagcg gccggcggcg gcggtccgga cggcgagcgg gaagccggcg tcccgccacg
     8221 ccgtccgccc ggcctcgtcc atgtcccacc acagcagctg cgccccgtgc ccgacctgcg
     8281 ccatctcctt gcccgtcgac atgtgcagcc cggggttgag ccagagcagg gcacagccgg
     8341 gtccggcgtc gccgaccggc tgtccgcgat cctccagttc cgtgcccgac acctggagct
     8401 tcgacaactc cctgggccag ccgtccagtg gcaccggcgg gaacacccgc acctcggcac
     8461 cgcccccggc cgccgtgatg ccgggcagct ccgacgcctt gcgccactcg gcgccgcgcg
     8521 ccctgcgtac caccttgcgg atccggccgt cctgccagtc ccgtacgcgc gccgcccatt
     8581 cgccgtcccc ggacgaccgc gcgtcggaga gcatcagcac gaccgcgcgg gcggcggtct
     8641 ccagcgcgtc ggtgcgggac ggcggctcgt tccgctcgat ccggaccacg agcggcagga
     8701 cgaactgggg ggcctcatcg cgcggcaccg gctcctgacg caaggggctg ttggtgctgc
     8761 cactgctgtc cgtgctgccg gtgctgccgg tgctgcccgc gctggccgtg ctgtccctgc
     8821 tgttcacccg cgcattctgc cagcctgccg gaaggcaagg gggcccggcc cgcccgggtg
     8881 gggacgcggc cggccgccca gtctctgacg ttgcgtcaaa aaatattctt tgcacgggtg
     8941 atgaacggac acggggacgg cgtccgtatc aagggccgga cgcgttcccc ccctcccggt
     9001 cccccctcgg cgaccggagt ccgtaccccc cgcaggaggc gaagagttga gtcacaggcg
     9061 tatacccaag cggaaggcga tactcgccgg agccggagcg gtgggcatcg ccgcagccgc
     9121 agtactgctg ccgaatgcca acgcttcgca gtccgggtcc gaggacgagc ggaccgctcc
     9181 cagaaagctg agtgcggcat ctgccgcgga tctcctccag cagctgggca ggcaactcgg
     9241 cgagtcctac ggcggcgcgt actacgacgc gtcgaagcag cagctcatcg tgaacatcgt
     9301 cggcgatgac aacgatgtgg agatcgaggt caagcgggcc ggtgcggtgg cccgcagcgt
     9361 gcagaacagc ttctccaccc tgaagtccgc cacccgtacg ctctccagcg aggccacggt
     9421 gccgggcacg gcctgggcca tcgaccccag gaacaaccag atcctggtga ccgccgaccg
     9481 caccgtcacc ggcgaacggt ggaacaccgt cgagtcggcc gtggagtcgc tgggcgacgg
     9541 ggtcgcccgg atccagaagt cgaagggcga gttcaagccc ctgctggagg gcggcgacgc
     9601 catcttcagc aacggcggtg cgcgctgctc gctcggcttc aacgtcacga cccaggacgg
     9661 gcagtccggc ttcctcacgg caggccactg cacagccgcc tccgagcagt ggtccgagca
     9721 gcagggcggc cagccggtcg ccaccgccca ggagtccgtc ttccccggtg agggcgactt
     9781 cgcgctggtg acgtacgacg acccgaacac cgaggcaccg agcgccgtcg acctcggcga
     9841 cggacgggcc ctggagatcg ggcaggcggc agacgcggcc gtcggccagg aggtcttccg
     9901 catgggcagc accaccggcc tcaacggcgg gcaggtgacc gggctgaacg ccaccgtgaa
     9961 ctacgccgag ggcaccgtca ccggtctgat ccagaccaac gtctgcgccg aaccgggcga
    10021 cagcggcgga gcgctgttca ccagggacgg caacgccatc ggcctcacgt ccggcggcag
    10081 cggcgactgc acctccggcg gcgagacctt cttccagccg gtgacgaccg cgctggaggc
    10141 ggtgggcgcg cagatcggct gacgcgggcg tacgggtacc gccgccggcc gcgccgctcg
    10201 cggaccgtac gggggcggcg ctgacgcccc ggccgctcac ggaccgcacg gccggcccgt
    10261 acgggggcgt accgcccgtc gggacgcggt ggcagggacg gccccaaccg gacccccgcc
    10321 gcgaccccgg ccggagccgt ggtcggagcc ctcgtcggaa acctcacgga agccgaccgg
    10381 ggtcccggcc gcgacccaac cggaccccaa ccggaccccg gtcggaccca ggtcgggacc
    10441 ccgaccggac cccggccccg gctccggctc gggccggaag gggagagcga gggcaggagg
    10501 agaggtggcg gcacggcccc gggcatcggc ggatgcccgg ggccgtgcgg ctgcccggac
    10561 cgccgtgcgg ccccggccgt tcgccggcag ccccctgccc gccccgacac cgaaccggtc
    10621 cttcgggcgg tgcggggttg gtgttcccga ggtgttccgg gggcagcccg ccgaccgtcc
    10681 aggtgcgctg ccttccgccg cccaggtgcg gtaaccgccg cggccggggt gcggcggaga
    10741 cgtcggccgg gagcgatcgc ggtgcgcccg cctccgccgg ggcccgcccg gcccgtagcc
    10801 cggcctatag cccggcctgt agcccgggcc tgtagcccgg ccgggccccg gccggggccg
    10861 gagggagctg gagaccggcc cgcagccggc gccggcccgg cggagcaggc gctcagtgcc
    10921 aggggccggt caccgcgaac gtcgtaccgg gcgtgtagca gttgacgtac atggtcccgc
    10981 cgtccggtga gaacgtcacg cccgcgaact cgccccactc gggttcctcc ggggtgccgg
    11041 tgttctggcg gttgcgcgcc atcgcgtaca cttcgccccg cctggtcagc ccgtacacgt
    11101 gctgggcgcc gttgccgtcc tcgcacacca tcagcccgcc gcccggggcg aggcagatgt
    11161 tgtccgggga ctcaccgggc agttgcaggt cggtcgccgg gccgaagacg acgaccaggg
    11221 tcagccgccg ccgctccggt tcgtaccgcc acacctgccc gaagtggtcg gcggccgacc
    11281 cgtcggtgct gcgggcgaaa ctgcacacga agtagacgga cgagccgccc cagtagcagc
    11341 cctcgagttt ctgggcgtgg gtgatgcccc tcgggccgaa gtcctgtagc cgcacggggg
    11401 tctgacgcgc ctgcggatcg ggcacgggga cccactggac tccgtcgaag cgtgccccgg
    11461 tctcctggat cgcggacagg tccggcaccc ccgggacccg catcgcctcc agccgtccgc
    11521 ccgcgcgcag cgagccggtg ccgccgagcg gtctctccgg caggaagcgg tagaacagcc
    11581 cgaacggccg gtcgaaggcg tcctccgtct c
//
