LOCUS       NZ_CP029254            10294 bp    DNA     linear   CON 23-OCT-2024
DEFINITION  Streptomyces spongiicola strain HNM0071 chromosome, complete genome.
ACCESSION   NZ_CP029254
VERSION     NZ_CP029254.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces spongiicola
  ORGANISM  Streptomyces spongiicola
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7180417)
  AUTHORS   Zhou,S. and Huang,X.
  TITLE     Complete genome sequence of the Type Strain of Streptomyces
            spongiicola HNM0071, the producer of staurosporine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7180417)
  AUTHORS   Zhou,S. and Huang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2018) Institute of Tropical Agriculture and
            Forestry, Hainan University, No 58, Haikou, Hainan 570228, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 850.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003122365.1-RS_2024_10_23
            Annotation Date                   :: 10/23/2024 01:04:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,921
            Genes (coding)                    :: 5,739
            CDSs (with protein)               :: 5,739
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 47 of 182
            Pseudo Genes (incomplete)         :: 161 of 182
            Pseudo Genes (internal stop)      :: 9 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:10:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3073234
            Orig. end                         :: 3083528
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029254.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10294
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="11"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10294
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10294
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5294](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5294
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(468..1433)
                     /locus_tag="DDQ41_RS13400"
                     /old_locus_tag="DDQ41_13400"
     CDS             complement(468..1433)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018510823.1"
                     /locus_tag="DDQ41_RS13400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_13400"
                     /product="EamA family transporter"
                     /protein_id="WP_394342183.1"
                     /transl_table=11
                     /translation="MAAATVALPEAAGGLRSGELGPGELGSGELGPGARRARGSLGPVA
                     LVVAGGLSVQFGAAVAVLLMPRAGALGVVTLRLVLAAVVLLLVCRPRLRGHSRDGWGTV
                     VAFGAAMAGMNMLFYQAVDRIPLGAAVTLEVLGPLALSVIVSRRLVNLVWAGLALGGVV
                     LLSGGGFDRLDPVGAAFALGAGVMWAAYIVFSARTGRRFPQADGLALAMAVGAVLSLPF
                     GLAEAGGKLLVPSTIGLGLLVALMSSVLPYTLELLALRRLPAPTFAVLMSLEPAIAATA
                     GFLVLHQALSAGDALAIALVVAASMGAVRTQVGAGRTARK"
     gene            1653..2510
                     /locus_tag="DDQ41_RS13405"
                     /old_locus_tag="DDQ41_13405"
     CDS             1653..2510
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889581.1"
                     /locus_tag="DDQ41_RS13405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_13405"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_109294714.1"
                     /transl_table=11
                     /translation="MSIELRHLRCFLAVAEERSLTRAATALHLTQPAVSRTLAALERHL
                     GVRLVDRSTHHLHLTAEGRAFHEQAAAAVAAFDDALDPARLRRRPLRLGHAWSAFGAFT
                     TPLLRRWQREHPETPLELLRIDDRTAGLARGEVDAAVLRGTVDTPGLVTAPLFTEPRVA
                     AVTSDGPLAGRTSLTLADLAAVPVILNTVSGLTTPDLWPAHSRPAATLTVGNTDDWLTA
                     IAAGRGAGASAASTAEMHPHPGVTYRPLTDAPPVPVLLAWRATAPHPALGALAALAREL
                     GAEG"
     gene            complement(3123..4583)
                     /locus_tag="DDQ41_RS13410"
                     /old_locus_tag="DDQ41_13410"
     CDS             complement(3123..4583)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469705.1"
                     /locus_tag="DDQ41_RS13410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_13410"
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="WP_109294715.1"
                     /transl_table=11
                     /translation="MLIRLRSRRSVLVWLVPFLWTVVLGLWGLSREGSVWRDEAATWQV
                     ALRSTRDIARMLEHVDVVHALYYLFMHALFEGFGPSTTVLRLPSVLAVAAGAACVAEIG
                     RRLSGNPVGLGGGMAVGLIPSVQFHLQEGRPYALVAAGAGLSTLLLVSILQGGTNRWGR
                     WAAYGCTVLVCGLLNWLSLLILPAHLATLVWARAERGTWVRWAAASTAATAGVTPLIFF
                     SRTQSGQVSWIPPLTWHMLIGPAVLLAIAGVGTLLDRPRARRLSAAAVGLPLLAVPQLG
                     LIGLSLFKPLFVDRYVLFSLLGLALMIGVVLGALVGAVQRRSRNAAHWVVPVAVAVASV
                     ALLPQAIAKRSPHSRVDDVLAAAQRVERWERPGDAVLFVPAARRDTKLVSPHAFTGLQD
                     IALEDTPERSATLKGVERDAARIRAEILARQRVILVTDAEDVSRPASSARDRAKMSTLR
                     EHFRPVDDEQVRGRRVLVYERCTPGPPS"
     misc_feature    complement(4329..4331)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(4548..4550)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(4799..4813)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     gene            5001..5294
                     /locus_tag="DDQ41_RS13415"
                     /old_locus_tag="DDQ41_13415"
     CDS             5001..5294
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.5"
                     /locus_tag="DDQ41_RS13415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_13415"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_109294716.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 7.9e-10,
                     bitscore: 37.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKRTALKATLASAALILGAAAPAMAITVDVGGGRWTYDQGANSA
                     WSNYYHSANKHASSVKIGANIFRSGCTSAGSWSLASGGKNGGAINWYYDPSC"
     misc_feature    complement(5407..5423)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.34"
                     /tool="antismash"
     gene            5538..7568
                     /locus_tag="DDQ41_RS13420"
                     /old_locus_tag="DDQ41_13420"
     CDS             5538..7568
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DDQ41_RS13420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DDQ41_13420"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_162602674.1"
                     /transl_table=11
                     /translation="MRSLDESRVLGSVALVDVIDSDASASSARVVDVVESVVSGSRIGV
                     AREIPDLKNPEGLRHLYLATGDRGSATAAWMDEGYPSFSRNVETRVHPLSDIKHLDPRG
                     AYHVFGPPEAVDALVAEFGKLGLRVNVAYPLSYTTLATDYAGDPLPWSLGVMALAVVTL
                     TGASVLLRAKTYAVLRLQGMSYTDILVRDLRQTAGFRLVSSGGVVAVALGVLRLYNDWA
                     RLGLFASIALGIAALLTVLVLASHAAVLALVFRVGILSSLKGELPTRAATISLYVVRVP
                     ALLLALSIALSVAMAGQNVLKRQENRDAYRSVGEAVSIRLSSAFATRPDELDRKVGPWL
                     RAADTRGEIVVAGRGDLQMAAPNANLPAGEILIANETYVREQQVLDQEGRRYVPEAGGT
                     KTSQPRPVRVLIPASYAPHTKAITKAVSDKLDPGRGRNIPVETLGAKNGQRLFGYNTGA
                     LVYSSTHPANEDRSLMRDPIVVVVPNGSDFLTDDAYVTFATQEGVVFPDPGDVLSAIES
                     VGLEDYVSSVSPVGQRIAAELSDAVGDFRMQMFNLALAVTVLLVAGVGICIIYTRKNAQ
                     AIFVRHFSGWTFAASHRFVLAVETAIAIVFAIRIPIEVWQQNREIAEFTEAGLPAPFPP
                     LHVEFADLSVIAGLAVVELAVILLALSALHRRIVKEGSSEA"
     gene            7620..8276
                     /locus_tag="DDQ41_RS13425"
                     /old_locus_tag="DDQ41_13425"
     CDS             7620..8276
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006374867.1"
                     /locus_tag="DDQ41_RS13425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_13425"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_109294718.1"
                     /transl_table=11
                     /translation="MIDIEGLSKKFGERTLWHDVSLTVGQGEMLALIGPSGSGKSTLLN
                     CLGLLDRPNAGAIRHEGRDITRFSRREARLFRRDVLGYLFQGYALIENATVEENLEVAV
                     KPRRALRGKTGQSLGQALQRVGLAGRHKETVHRLSGGEQQRVALARLIVQQPTLVLADE
                     PTAALDRDNTAMVLDSLREMSRSGCAVVIATHDDTVREACDNVFAVGAPLASQSS"
     gene            8799..9629
                     /locus_tag="DDQ41_RS13430"
                     /old_locus_tag="DDQ41_13430"
     CDS             8799..9629
                     /EC_number="3.1.3.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1179: hydrolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889575.1"
                     /locus_tag="DDQ41_RS13430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_13430"
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="WP_109294719.1"
                     /transl_table=11
                     /translation="MRHPRSRPRLIATDLDGTLLRRDGTVSERTLRALRTAVEGGADIV
                     FVTARPPRYVDALATATGLAGTAVCSNGAQVYDVRARTVIRSRTLVPDTARRVATVLAE
                     AAPELGFAVETGLRVLYEPSYLLRFSGGLAAQSPVASLGDLWLLDVPIVKLLAHSHRCD
                     ADVLVALAEEAAGGEAQFTHSGGRGLLEVSARGVTKAAALSALCAERGVTAGEVVAFGD
                     MPNDLAILRWAGSGYAMGNAHPSVLAAVPRRTASNEDDGVALVLERLFGEARPR"
     misc_feature    9872..9887
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 19.67"
                     /tool="antismash"
     misc_feature    10209..10229
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.8"
                     /tool="antismash"
     misc_feature    complement(10271..10285)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.44"
                     /tool="antismash"
ORIGIN
        1 atcgacctgg cgctcatggg cggcccgtac caggggaagc gggcgcccgg gggcgcttcg
       61 tgcagcgcct gccggagccg ttcgcggccg gtgcgccagt gggcgagcag ttcggcgggc
      121 ggccggcggg ccccggcctc ggcggcccgg tcgacgaagg cgtccggctc ggcgagcgcc
      181 ttctcgacct cccgggcgaa ggcgccgggg ctggtggcgg cgagcagcgc gacctcgtcg
      241 gtccaggcga ggtgggcgat ctggtgggcg acggtccagc cggcggcggg ggtcgccgtc
      301 ccccattcct cgtcgctcga cccggccacc agccggtcga gttcgtcgct ctctgcgcgc
      361 aggtcgtcga tgacggccgc ggggtcggac acggggcgct ccctccggga cacggcgtgg
      421 tgcccggagc atgccagcgg acagcgaaac aagcaagcgt gcttgcttta ctttctcgcc
      481 gtacggcccg cgcccacctg ggtgcgcacc gcgcccatac tcgccgcgac gaccagggcg
      541 atcgccagcg cgtcgccggc ggacagcgcc tggtgcagca cgaggaaccc ggccgtcgcg
      601 gcgatcgccg gctccaggct catcagcacg gcgaaggtgg gcgcgggcag ccgccgcagg
      661 gcgagcagct ccagcgtgta cggcagcacg gacgacatca gcgcgaccag gaggcccagc
      721 ccgatcgtgg acgggacgag cagcttgccg cccgcctcgg cgaggccgaa cggcagactg
      781 aggacggcgc cgaccgccat cgccagggcc agcccgtcgg cctgcgggaa gcggcgtccg
      841 gtgcgggcgc tgaacacgat gtacgccgcc cacatcacac ccgcgccgag cgcgaacgcg
      901 gcaccgaccg gatcgagccg gtcgaagccc ccgccgctca gcaggacgac gccgccgagc
      961 gcgaggcccg cccagacgag gttcaccagc cgccgggaga cgatcacgga gagcgccagc
     1021 gggccgagca cctcgagggt caccgcagcg cccagcggaa tccggtcgac ggcctggtag
     1081 aacagcatgt tcatgcccgc catcgccgcg ccgaaggcga cgaccgtccc ccagccgtcg
     1141 cgggagtggc cgcggagccg gggccggcag accagcagca gcacgacggc ggcgaggacc
     1201 agccggagcg tgacgactcc gagcgccccc gcgcggggca tcagcagcac ggccacggcc
     1261 gcgccgaact gcacggacag acctccggcg acgaccaggg ccaccggccc gagcgaaccc
     1321 cgggcgcgcc gtgcacccgg ccccagttcg cccgacccca gttcacccgg ccccagttca
     1381 cccgacctca gcccaccggc ggcctccggg agggcgaccg tggcggccgc cacccgctcc
     1441 gcgccctcgc ggccgccacc acccggggac gcatccgcgc cacggagcct gcccatcgat
     1501 cccccttcgt ccacttgaat aaacccgcaa ttcaaaatac tgcaccggaa gccactgcac
     1561 aggacgcacc cgccccacct cgcccccttc acgctacgga agcccgcgcc acccgtgaaa
     1621 tgccgattgg tctgcggtta tgctccgcac acatgagcat cgagctgcgt catctccgct
     1681 gcttcctcgc cgtcgcggag gaacggagcc tgacccgggc cgccaccgca ctgcacctca
     1741 cccagccggc cgtctcccgc acgctcgccg cgctggaacg gcacctcggc gtgcgcctcg
     1801 tcgaccgttc cacccaccac ctgcacctca ccgccgaggg ccgggcgttc cacgagcagg
     1861 ccgccgccgc ggtggccgcc ttcgacgacg ccctcgaccc cgcgcggctg cgccgccggc
     1921 ccctgcgcct cgggcacgcc tggtccgcct tcggcgcgtt caccaccccc ctgctacgcc
     1981 gctggcagcg ggaacacccg gaaacgccgc tggaactcct ccgtatcgac gaccgcacgg
     2041 cggggctggc gcgcggcgag gtggacgccg ccgtgctgcg cggcacggtc gacacccccg
     2101 gactggtgac ggcaccgctg ttcaccgagc cccgggtcgc cgccgtcacc tccgacgggc
     2161 cgctcgccgg ccgcacctca ctgacgctcg ccgatctggc cgccgtcccg gtcatcctca
     2221 acaccgtctc cgggctgacg acccccgacc tctggcccgc ccattccagg ccggcggcca
     2281 cactgaccgt cggcaacacc gacgactggc tgacggcgat cgccgcgggc cgcggcgccg
     2341 gggcgtccgc ggcgtcgacg gcggagatgc acccccaccc gggcgtcacc taccgtccgc
     2401 tcaccgacgc acccccggtc ccggtgctcc tcgcctggcg cgccaccgca ccgcacccgg
     2461 cgctcggagc actggcggcg ctggcgcgcg aactcggcgc cgagggctga agggaggcgg
     2521 cacggcgacc ggtcgcgact cgggccgacc cggccgcccg ttgcgccccg gagcggtcgg
     2581 ttgcacgcct acgcggtctg ttgagccccg acgcgacccg accggccccg tgccgcgtcg
     2641 ggcagggctt gcccgtttct cacactctta cgcccccgta ctgacaagcg ccgtcacctc
     2701 gaccaggccg agccaggcgg acgcaatcgg caccggcaga ggcccggtgt ccggcgcggc
     2761 caccgaccgc atcgccgggg ccagttcgct ctgtggcgct tcctcctcac ccgtaccgtg
     2821 aacctacgcc cgcagagtct cggcctcggc agcccgtcgc cgccggaagg cggcctgccg
     2881 gcacttcgcc gaacacgtct gccgcgtccc gctacggccc tgcaccaact ccgtgccaca
     2941 gatctcgcac cgtgccgtcg caaccctctc gggttcgaca ggtaacaatc cggaacgatg
     3001 cagaggtgcc cccgtctaca tcccccaccg gaatggaacg cggcctcggg gttcgtcggc
     3061 gcacccctcc ttcggctcgg cgacgggtac cacgcccgct cggacggggc caggctccga
     3121 agtcaggacg gcgggccggg tgtgcacctc tcgtacacga gcaccctcct gccgcggacc
     3181 tgctcgtcgt ccaccggccg gaagtgctcc cggagcgtgg acatcttggc cctgtcccgc
     3241 gccgaactcg ccggcctgga gacgtcttcc gcgtcggtga cgaggatgac gcgctgccga
     3301 gcgagtatct cggcccgtat gcgggcggca tcccgttcca ctcccttcag cgtcgcggag
     3361 cgttccggcg tgtcctccag agcgatgtcc tgtaggcccg tgaacgcgtg cggggagacc
     3421 agcttggtgt cccgccgcgc cgccgggacg aacagcacgg cgtcgccggg cctctcccac
     3481 cgctccaccc gctgcgcagc cgccaggacg tcgtccaccc ggctgtgcgg ggagcgtttc
     3541 gcgatcgcct gcggcaacag ggccaccgag gcgacggcga ccgcgacggg aacgacccag
     3601 tgcgcggcgt tccgcgaccg gcgttgcacc gccccgacca gcgcgccgag tacgacaccg
     3661 atcatcaggg ccaagcccag caggctgaag agcacatacc ggtccacgaa gagaggcttg
     3721 aacagcgaga gcccgatgag gccgagctgc gggaccgcga gcaggggaag accgacggcc
     3781 gctgccgaca gccgtcgcgc ccggggccgg tccagcaggg ttccgacgcc tgcgatggcc
     3841 agcagtaccg ccggcccgat cagcatgtgc caggtcagcg gaggtatcca ggagacctgc
     3901 ccggactggg tacggctgaa gaagatcagt ggcgtcacac ctgccgttgc cgccgtggat
     3961 gcggcggccc agcgaaccca cgtcccgcgc tccgcccgtg cccataccag ggtcgccagg
     4021 tgcgcgggca gtatcagcaa cgagagccag ttcagcaggc cgcacaccag gaccgtgcag
     4081 ccgtaggcgg cccagcgacc ccaacggttc gttcccccct gcagtatcga cacgagcagc
     4141 agtgtcgaga gccctgcgcc cgccgccacc agcgcgtacg gacggccttc ctgaaggtgg
     4201 aactgcacgg agggaatcaa cccgaccgcc atgcctccgc ccagccccac cggattcccg
     4261 gaaagccgcc gcccgatttc cgccacgcat gccgctcccg cggcgaccgc gagcacggaa
     4321 ggcagccgta acacggtggt cgaagggccg aacccctcga acagcgcgtg catgaacagg
     4381 taatagaggg cgtggacgac gtcgacgtgt tccagcatgc gcgctatgtc cctcgtcgac
     4441 cggagtgcga cctgccaggt cgcggcttcg tcacgccaga cactgccttc cctggacagg
     4501 ccccacaaac cgagcacgac ggtccacagg aaagggacaa gccagactaa gacactccgc
     4561 cgagagcgga ggcggatcaa catcttcaaa gtcggtcgcc ccccggccgg ccaaatcaac
     4621 gcagtcgttc gcaaatcgtg gcgcacggcg cgaatccggt gacgggcagg ccgccgacag
     4681 cgcctgggtg atcctatccg aaaggatcgc cacagggcgg ggaggtggcg acatacaggc
     4741 gtcggggctc catgcgaggc gacgttttca cgccatgaag gcctcgaatt gacgcaaaat
     4801 cgagagacgt gaggcaaaac ggatcagaga attccggagt caacttcgcc gagtcgggcg
     4861 ggaatggccg attccgccat caggatgcgg cggaatagtg acggtgatcc aggtcacccc
     4921 aatactcggt tgacggttca tcgaggcatt gacaaagatc tcgactcgga aaatcgagca
     4981 acacacaagg ggaagcgttc atgaagaaga gaactgctct gaaggccacg ctcgcctctg
     5041 cggcgctgat tctcggtgcg gccgctccgg cgatggccat cacggtggac gtcggcggcg
     5101 gccgttggac ctacgaccag ggcgcgaaca gtgcttggtc gaactactac cactcggcca
     5161 acaagcacgc gtcctcggtg aagattggcg ccaacatctt ccggagcggt tgcacttcgg
     5221 ccggtagctg gtcgctggcg tccggaggta agaacggtgg cgccatcaac tggtactacg
     5281 accccagctg ctgattcacc gcgctcagat cgtgagagtg tgcagccggt agattccacc
     5341 gagtaagccg aagctccggt ccgacgcggc tacgggtgta cagtcctact acccgtagcc
     5401 gtgtcttgcc tctctttgtt tcacctgaca tgcacacccc ccggaacttc gaaataagat
     5461 gggcaatgct acagcgaggt atcaggttcg ttcacgtcgt cacactgctt ttctccgcat
     5521 tgcttgcctt cctcttcgtg cgaagcctcg acgagagccg agttctggga agcgtcgccc
     5581 ttgtcgacgt gatcgactcc gatgcctcgg cgagttccgc gcgcgtcgtg gacgtcgtgg
     5641 aatcggtcgt gtccgggagt cgaatcggcg tggcccgcga gatcccggat ctgaagaatc
     5701 ccgaggggct ccggcacctt tacctcgcga cgggagaccg cgggtcggca actgcggcct
     5761 ggatggacga ggggtacccg tcgttcagcc ggaacgtcga gacccgtgtc catcccctgt
     5821 ccgacatcaa gcacctcgat cctcgcggcg cgtaccacgt cttcgggcct ccggaagccg
     5881 tcgacgccct ggtcgccgag ttcggcaagc tcgggctccg tgtcaacgtt gcgtacccgc
     5941 tgtcctatac gaccctggcg accgactacg ccggcgatcc cctgccctgg tccctgggcg
     6001 tcatggccct cgccgtcgtg accctgaccg gggccagtgt cctgctccga gccaagacgt
     6061 acgccgtcct tcgcctgcag ggcatgtcct acaccgacat cctggttcgc gatctgaggc
     6121 agacagcggg cttccggctc gtctcctcgg gtggcgtggt ggcggtggcg ctcggtgtcc
     6181 tgcgcctcta caacgactgg gccagactcg gcctgttcgc ctcgatcgcc ctgggaatcg
     6241 ccgccctcct gaccgtcctt gtgctcgcct cccacgccgc ggtactcgcg ctcgtcttcc
     6301 gggtcggaat cctctcgtcg ctgaagggcg aactgcccac acgagcagcg accatcagcc
     6361 tctacgtggt ccgcgtgccg gcactcctcc tcgctctgag catcgcgttg agcgttgcca
     6421 tggccggtca gaacgtcctg aagcggcagg agaaccgcga cgcctaccga agcgtagggg
     6481 aggccgtgtc gattcgtctc agcagtgcgt tcgccacccg acccgacgaa ctggaccgaa
     6541 aggtcgggcc gtggctccgg gcggccgata cgcgaggcga gatcgtcgta gccggccggg
     6601 gagacctcca gatggcggca ccgaatgcga acctgccggc aggcgagatc ctcatcgcga
     6661 acgagaccta cgtccgtgag cagcaggttc tcgaccagga gggccgacgt tacgtccccg
     6721 aagccggcgg cacgaagacc tcgcagcccc gccccgtgcg cgtgctcatc cccgcctcct
     6781 acgcacctca cacgaaggcg atcaccaagg cggtctccga caagctcgac ccagggcgtg
     6841 gccggaacat ccccgtcgag actctcggag cgaagaacgg gcagcgactg ttcgggtaca
     6901 acaccggtgc cttggtctac agctcgacac accccgcgaa cgaggacaga tcactgatgc
     6961 gcgatccgat cgtggtcgtg gtccccaacg ggtcggactt cctcaccgac gacgcctacg
     7021 tcaccttcgc gacccaggaa ggcgtcgtct ttcccgaccc cggcgacgtc ctgagcgcca
     7081 tcgaatcggt cgggctggag gactacgttt cgtccgtctc cccggtcgga cagcggatcg
     7141 cggcggagtt gagcgacgcg gtcggcgact tccggatgca gatgttcaac ctggccctcg
     7201 cggtgacggt cctgctggtc gccggggtcg ggatctgcat catctacacg cggaagaacg
     7261 cgcaggccat cttcgtgcgt cacttcagcg gatggacgtt cgccgcatcc caccgcttcg
     7321 ttcttgcggt ggagacagcc atcgcgatcg tcttcgcgat acggattccc atcgaggtgt
     7381 ggcagcagaa ccgtgagatc gccgagttca ccgaggcagg tctgccggcc cccttccccc
     7441 cgctgcacgt cgagttcgca gacctgagcg tcatcgccgg cctggccgtg gtcgaactcg
     7501 ccgtgatcct cctcgctctg tcggcgctgc accgccgcat cgtgaaggaa ggctcgtccg
     7561 aggcgtgacc cctcgagaac cgaagcaacc cccgcccccc atccagccgg agtgagattg
     7621 tgattgacat cgagggactg tcgaagaaat tcggcgaacg gacactgtgg cacgacgtca
     7681 gcctgacggt cggtcaaggc gagatgctcg ccctcatcgg tccgagcggc tctggcaagt
     7741 cgaccctcct gaactgcctg gggctgctcg acaggccgaa cgccggagcc atccgccacg
     7801 agggcaggga catcacacga ttcagcaggc gtgaggcacg cctcttccgc agggatgttc
     7861 tcggctatct cttccagggg tacgccctca tcgagaacgc gaccgtggag gagaacctcg
     7921 aagtggccgt aaagccacgc cgtgcgctac ggggcaagac cggacagtcc ctcgggcaag
     7981 cactccagcg cgtcgggctg gccgggcggc acaaggagac agtccaccgt ctcagcggtg
     8041 gcgagcagca gcgagtggcc ctcgcccgcc tcatcgtcca gcagccgacg ctcgtcctcg
     8101 cggacgagcc gaccgccgcc ctcgaccgtg acaacaccgc catggtcctg gactcgctcc
     8161 gggagatgag cagatccggg tgcgcggtgg tgatcgccac ccatgacgac accgtccgcg
     8221 aggcctgtga caacgtgttc gccgtgggag cgccgcttgc gtcccagtct tcctgaatcc
     8281 ggccgcgacc gcggcacagg gcgcaacgca gcccggcgcg ctgggcgcgg gatccgggcc
     8341 gcggcgggca ctccacggca tgggaacggc cggaaccaga ccggcccttg gctctgcagg
     8401 ggacaaggcc gcctcgcgcc tccgagatcg tctcctccgc tgcttcgatg cggcgatgcg
     8461 tgcagcttca gccgggcacc ctgcgtacct ccacgatggt gtgtccgtgc cctggaaacc
     8521 tcgccgctcc ggcaagcatg ggaccgaggc ggaactgtcc ggcgccgcgg acgggaagtt
     8581 cgtcgggacg accagcgtgt gcgagaggcc gaacgtgatc gggttcttgt acatgaggat
     8641 cgcctcgatc gggtcgtcac ccaccaggcg tttcaccgtg gcctcggcga agtggcgggc
     8701 tgacccggtc gatgcggcaa cgcagcgagt cgccgggccg gccgcgccgg acatcacgac
     8761 ggggcgcatg tcgcctgacg cggccctagc atgggccgat gcggcaccct cgctcccgcc
     8821 cccggctgat cgccaccgat ctggacggca cgctgctgcg ccgcgacggc accgtgtcgg
     8881 aacgcaccct gcgcgccctg cggacggcgg tcgagggcgg ggccgacatc gtcttcgtca
     8941 ccgcccggcc gccgcgctat gtggacgccc tggccacggc cacgggactc gcgggcacgg
     9001 cggtgtgcag caacggcgcc caggtgtacg acgtgcgggc gcgtaccgtc atccggtccc
     9061 ggactctggt accggacacc gcccgcaggg tggcgaccgt cctcgccgaa gcggcccccg
     9121 agctggggtt cgccgtggag acgggcctga gggtgctgta cgaaccctcg tatctgctgc
     9181 gtttcagcgg cgggctcgcc gcgcagtcgc cggtggcgtc gctgggcgac ctgtggctgc
     9241 tggacgtccc catcgtcaag ctgctcgccc actcccaccg gtgcgacgcg gacgtgctcg
     9301 tggcgctggc ggaggaggcg gcgggcgggg aggcgcagtt cacccactcg ggcgggcgcg
     9361 ggctgctgga ggtcagcgcg cggggcgtca ccaaggccgc cgcgctgtcc gcgctctgcg
     9421 cggaacgggg cgtcaccgca ggggaggtgg tggcgttcgg cgacatgccg aacgacctgg
     9481 cgatcctgcg ctgggcgggc agcggctacg cgatgggcaa cgcgcacccc tccgtactcg
     9541 ccgcggtccc ccggcggacc gcgtccaacg aggacgacgg cgtggccctg gtcctggagc
     9601 gcctcttcgg cgaagcccga ccccggtgag cctcggctga cgtccccgac ccgaccaacc
     9661 ccgttggaga acggggcata cacgtcggcc cggggccgtg cccggcagcg tgaaattctg
     9721 tacggcggcc ccggagccgc gtgggctccc gtctcagtcg cgggggaact gcccggtcgc
     9781 gagggtgaga tcgacgggcg tgccggtgag gtcgacgtcc gcgccgaagg cgacggtggt
     9841 ctccacgagg tagtcgccgc ccttgggctg ggtgtacacg tggcaacggc cgacgtaggg
     9901 gtcggcgatc aggtagaccg gcacctcggc ggtcgcgtag gcccgcttct tggggccgta
     9961 gtcgttggcg ccggtgctcc tggagacgac ttcgacgacg aagaccacgt cctcatgaca
    10021 ccactgccct ttggcgttct tctcggcgtc tcccctgaga agggtcaggt cggtggcgaa
    10081 cccgttcagg tggccggggt agtcgatgcg aacgtccgac ttgatccttt tccgggggta
    10141 ctgccctcgc aactggtcgt agacgtccgc gatgatgtcc cagtgcgcgt cccgctgcgg
    10201 cgacatgaat agggtcccct cgacgatctc gaccttgtac ccctcgggga cgggcatccg
    10261 ctcgagcgcc tcgaacatct cgtccagcgt gcgc
//
