LOCUS       NZ_CP029254            10993 bp    DNA     linear   CON 23-OCT-2024
DEFINITION  Streptomyces spongiicola strain HNM0071 chromosome, complete genome.
ACCESSION   NZ_CP029254
VERSION     NZ_CP029254.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces spongiicola
  ORGANISM  Streptomyces spongiicola
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7180417)
  AUTHORS   Zhou,S. and Huang,X.
  TITLE     Complete genome sequence of the Type Strain of Streptomyces
            spongiicola HNM0071, the producer of staurosporine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7180417)
  AUTHORS   Zhou,S. and Huang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2018) Institute of Tropical Agriculture and
            Forestry, Hainan University, No 58, Haikou, Hainan 570228, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 850.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003122365.1-RS_2024_10_23
            Annotation Date                   :: 10/23/2024 01:04:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,921
            Genes (coding)                    :: 5,739
            CDSs (with protein)               :: 5,739
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 47 of 182
            Pseudo Genes (incomplete)         :: 161 of 182
            Pseudo Genes (internal stop)      :: 9 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:10:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1008081
            Orig. end                         :: 1019074
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029254.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10993
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="5"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10993
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10993
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5993](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5993
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            204..809
                     /locus_tag="DDQ41_RS04275"
                     /old_locus_tag="DDQ41_04275"
     CDS             204..809
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138908.1"
                     /locus_tag="DDQ41_RS04275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_04275"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_109293273.1"
                     /transl_table=11
                     /translation="MARPRQFDETEAVTSATELFWRRGYHATSVRDLGDELKLTPSSLY
                     RTFTDKHTLFLRALDHYRATESAEARQHLNAADRPVREALREWMLWLVGCPAGGKPGSG
                     CFVVNTATELGTADEKVSRRTEAAFEVTRQDLRSALAGGRDRGELSADLDIDAAVELLF
                     TTVLGLRVRERAGHDIERLTAAIDAAIRALGPAPAEPA"
     gene            complement(<887..1078)
                     /locus_tag="DDQ41_RS32220"
                     /pseudo=""
     CDS             complement(<887..1078)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013646.5"
                     /locus_tag="DDQ41_RS32220"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="polyketide oxidase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MFDLDLAEVSAEGPVIAGSPQAYAEHVFAERAGGLGARILRGQEC
                     TAVHDERVRVDLRSADGTR"
     gene            complement(1051..1515)
                     /locus_tag="DDQ41_RS32225"
                     /old_locus_tag="DDQ41_04285"
     CDS             complement(1051..1515)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DDQ41_RS32225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DDQ41_04285"
                     /product="hypothetical protein"
                     /protein_id="WP_262508348.1"
                     /transl_table=11
                     /translation="MVDGADTHRLDLLTVLRLLAEEAPLESYEELLLRAGQECSAAERL
                     RLEQAVRLAYVIHASQDRHRQREDALAELVDTASDMIRPYGPDELLYVITRRVKRIVNA
                     DLACIGLREDDGSLCTAAGCARRCSRDRRAGDGPASPSTSRGCSTSTSPR"
     gene            complement(1734..2126)
                     /locus_tag="DDQ41_RS32230"
     CDS             complement(1734..2126)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DDQ41_RS32230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_262508349.1"
                     /transl_table=11
                     /translation="MDGGPVHGVAKGPHLRSHAVLLPHSWQRAVDRWIEADFRLGRRRE
                     LCADLAGVLARFHSPAPLYTQLKADLDHDGQDPEVIRRYLRRRRGSPALTARRGVPDRV
                     APRTPRGGDLARSAAPPPGLPRLQRI"
     gene            complement(<2132..2602)
                     /locus_tag="DDQ41_RS32975"
                     /old_locus_tag="DDQ41_04295"
                     /pseudo=""
     CDS             complement(<2132..2602)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015649.5"
                     /locus_tag="DDQ41_RS32975"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_04295"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLLLVGSAIVEPAEGGDGDMDLRVLGGLAAHEGGVPISPSAVLPR
                     QVLALLAASADRVVPTCAIAEELWPQGAPAHAEQAMESHTRQLRADIGAALQAAGSDRS
                     AEDVLVRVPGAYRLDTGGGSSDARAFERTAGAGYRALEAGDLHLAGRRLREAV"
     misc_feature    complement(2591..2593)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(2597..2599)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    2602..2618
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.67"
                     /tool="antismash"
     misc_feature    2605..2614
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.91"
                     /tool="antismash"
     misc_feature    complement(2611..2624)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.6"
                     /tool="antismash"
     gene            2711..3415
                     /locus_tag="DDQ41_RS04300"
                     /old_locus_tag="DDQ41_04300"
     CDS             2711..3415
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133274.1"
                     /locus_tag="DDQ41_RS04300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_04300"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_109293276.1"
                     /transl_table=11
                     /translation="MRQERASRTRRALLRAAAEEFDRLGYAGASLAGIARAAGISLGAL
                     TFHFSSKQELSTAVSEQGCAATRALVARTGARGEPPVQCAVSLTLGLVELLEKDASVRA
                     AARLDREAADAGHGWQGVWAPVIHECLRAAADAEGSPVGDSAALAALAVLLVCGTEATL
                     PRPAGPGSPAGPAGHGGQQGNSALLARIWRQILPQTAQAGCGPGRPPQPRGSHTALHPP
                     STGTPAPVTNEA"
     gene            complement(3355..4086)
                     /locus_tag="DDQ41_RS04305"
                     /old_locus_tag="DDQ41_04305"
     CDS             complement(3355..4086)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (0-163). E-value: 3.1e-09. Score:
                     28.9. Matches aSDomain:
                     nrpspksdomains_DDQ41_RS04305_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892884.1"
                     /locus_tag="DDQ41_RS04305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_04305"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_245991568.1"
                     /sec_met_domain="adh_short (E-value: 5.7e-33, bitscore:
                     112.8, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.5e-30, bitscore:
                     105.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTGASSGIGEAAARLFAAQGAAVTVMARREDRLHALAEDISAAG
                     GSALVTAGDVTSPADVRRAVTAAVAMYGRLDAAFNNAGWGSVGSPLHETDDAVFEQIMD
                     VNVRGVWNCLKEQVPVMLRQGAGSIVNTSSTAGSFATGAIAPYVAAKHAVLGLTRAAAA
                     EYGDRGIRVNALMVGSTRTELMETAMEQVPRLEQAAVARAVQHRLADPGEVAEAALWLC
                     SDHASFVTGAGVPVDGGCSAV"
     aSDomain        complement(3598..4086)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DDQ41_RS04305_PKS_KR.1"
                     /evalue="3.10E-09"
                     /label="DDQ41_RS04305_PKS_KR.1"
                     /locus_tag="DDQ41_RS04305"
                     /protein_end="163"
                     /protein_start="0"
                     /score="28.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="MVTGASSGIGEAAARLFAAQGAAVTVMARREDRLHALAEDISAAG
                     GSALVTAGDVTSPADVRRAVTAAVAMYGRLDAAFNNAGWGSVGSPLHETDDAVFEQIMD
                     VNVRGVWNCLKEQVPVMLRQGAGSIVNTSSTAGSFATGAIAPYVAAKHAVLGLTRAAAA
                     "
     CDS_motif       complement(4018..4083)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DDQ41_RS04305_0001"
                     /evalue="6.60E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="DDQ41_RS04305"
                     /protein_end="23"
                     /protein_start="1"
                     /score="12.4"
                     /tool="antismash"
                     /translation="VTGASSGIGEAAARLFAAQGAA"
     gene            complement(4238..4867)
                     /locus_tag="DDQ41_RS04310"
                     /old_locus_tag="DDQ41_04310"
     CDS             complement(4238..4867)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270948.1"
                     /locus_tag="DDQ41_RS04310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_04310"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_109293277.1"
                     /transl_table=11
                     /translation="MAGRVIRQERAVQTRAALLRAAAEVFAESGYAGANVSAIAERAGL
                     TLGALYFHFRNKGQLAREIVLSQPDLVVPPHPCRGLQHAVDVTLTWAYQLLDDPLLLAG
                     ARLVMEQEHFAGPSQNSHQQWTRLVSEDLHEAKRRRELRAGVDVEALARLVVNACTGAQ
                     MHSHLETRRRDLPGRVEEMWRCLLPAIAVPSAVKGLRFGASRAKGV"
     misc_feature    complement(4885..4901)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.03"
                     /tool="antismash"
     misc_feature    4894..4909
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.62"
                     /tool="antismash"
     gene            5001..5993
                     /locus_tag="DDQ41_RS04315"
                     /old_locus_tag="DDQ41_04315"
     CDS             5001..5993
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270946.1"
                     /locus_tag="DDQ41_RS04315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_04315"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_109293278.1"
                     /sec_met_domain="AfsA (E-value: 4.9e-14, bitscore: 50.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHSSAHTDLRTSDLQRLARKASPGEVFVRDWREESPQTHVVALGW
                     PRSHTFYTLRSGATSPLLFVEAVRQALAVLSHTAQEIPLDHRLGRDSARCTFAPAAFRE
                     RRAPTDVELRVRHSGVTRRRLGSVRLTARIEATCGGEVLGCAEIGYATHPPVIYDRLRG
                     TYADAQQSFAAALPPARPVTPALVDRTHPGDVVIACTSKAHHYQLRTDTSNSVLFDHPH
                     DHVPGIVLLEAVVQAVHASAPGHARLASLDTAFLRYVEFDMPCWVDVAPGPPDAQGGSR
                     LLVTGTQNGRKAFSATLAFDSPTTSLEPAVPAGPERLGTGPTPAACPAG"
     misc_feature    5025..5027
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6125..8224)
                     /locus_tag="DDQ41_RS04320"
                     /old_locus_tag="DDQ41_04320"
     CDS             complement(6125..8224)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010276747.1"
                     /locus_tag="DDQ41_RS04320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_04320"
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="WP_162602616.1"
                     /transl_table=11
                     /translation="MRHSRSRRLAAFTTALAATPWALAAVPAQAVTGPAVAGSSYAFTA
                     RVEIGEGEDRRACSGALVDARWVLTAASCFTGGLAELAPGKPAEKTVATIGRADLTSTG
                     GHVSEIVDLVPRAGRDLVMARLAAPAAGITPVKISATPAGQGETVTVAGYGRTKTEWVP
                     DKLHTAGFTVGAVTDTGLDIVGKTAGDAICKGDTGGPLLRDNNGTPELVAVASRSWQGG
                     CFGQDAAETRTDAIAARADSTSLGSRLGTGQQLLPGDMLASATNRLTMQTDGDLVIASS
                     AGKVLWSTGTGGNPGATARFTGTGALQVVASDGTVLWQTGTTAPGGYVRLQDRGNLVVY
                     DAQDRSQWSSGTAVRHDYNGDGRSDLASWYEYPDQHDAVHTFLADQDGTLKAPFTAWSR
                     TVPGWDPSLMKITTGDYNGDGRGDLAVAYQYTAGVKLWTWTATSDGRFNAPFSSWEGDW
                     QLDRMTLHSGDFDGDGRDDIAAWYRYTEGNKLWTFPADAQGGFTAPSVSWSTSSWDVTN
                     CKFAVGDFDADGRDDLAVLYRYSDGSVKIWSFPASAAGGFGNPVESWTSTSWGDWARTD
                     FHAGDFDGDGRDDVATWYDYADGSDAIHVFAGASTEDGTFQAPRTAWNAVAGRFTRSSM
                     KLLAGDFDGDGRDDLATVYGYADSTVKMFTWTVKPDGTFNEPAAGWHSPAGGWFTRTQV
                     FGRYN"
     gene            complement(8302..8481)
                     /locus_tag="DDQ41_RS04325"
                     /old_locus_tag="DDQ41_04325"
     CDS             complement(8302..8481)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DDQ41_RS04325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DDQ41_04325"
                     /product="hypothetical protein"
                     /protein_id="WP_109293279.1"
                     /transl_table=11
                     /translation="MEILAVHAVRGDDCTTRVRMAVGSEEGSFATAENTWTGVGESADE
                     QGLEYMLVAIRSTK"
     gene            8949..9818
                     /locus_tag="DDQ41_RS04330"
                     /old_locus_tag="DDQ41_04330"
     CDS             8949..9818
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010470392.1"
                     /locus_tag="DDQ41_RS04330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_04330"
                     /product="hypothetical protein"
                     /protein_id="WP_316681334.1"
                     /transl_table=11
                     /translation="MATTVALVILALDPGRVVGWFSDSNRAEKPLGPESHRPIQAPSAE
                     GFRQPTLDEPFRDSPAARWANGTAGITVPEARATGWMSRAQVERALEQTRDFLAAANLD
                     PGALHGGHPEEAIALINPHQQDVQTYLKAAFHSPSEQNDPLLLFSRFDASRVRLVGDVV
                     KTRGRITYLEGERGALQVTSDVTYVYPVAPATGGDEVVRTIVRREVVLSWDDPAKVITE
                     PGTFSLVSYKVDMTNGGCDNSTGYLTPPFGTERAETGAGPDVDPYDRSTSMSERTTPDE
                     GCGTATRS"
ORIGIN
        1 ggtcaccagc aggaaggttc ccagggccac cgcggagacc gccatccagc ggcttctctc
       61 cgggacctct ggttccagtg cctgggcggg gttctgttct gcggtcgtca agggagcctc
      121 cccaataatt tgggagcgat cgctcccata cggtcgacac tagtatggga gcgatcgctc
      181 cgcaagtcgg gtaaggtgaa ctcatggcac gccctcgaca gttcgacgaa acggaagcgg
      241 tcacgtcggc gaccgagctg ttctggcgac gcggttacca cgccacctcg gtgcgcgacc
      301 tgggagacga gctgaagctc acgcccagca gcctctaccg gacgttcacg gacaagcaca
      361 cgctgttcct gcgcgccctc gaccactacc gggccaccga gtcggccgag gcccggcagc
      421 atctgaacgc ggcggaccgc cccgtgcggg aggcactgcg ggaatggatg ctgtggctgg
      481 tcggctgccc ggccggcggc aagcccggca gcgggtgctt cgtggtcaac accgccaccg
      541 aactcggcac cgccgacgag aaggtcagcc ggcggaccga ggccgccttc gaggtcaccc
      601 ggcaggacct gcgctccgca ctggccgggg gccgcgaccg cggcgagctg tccgccgacc
      661 tcgacatcga cgccgcggtg gagctgctgt tcaccacggt cctcggcctg cgggtgaggg
      721 agcgcgccgg ccacgacatc gagcgtctga ccgccgcgat cgacgccgcg atccgcgccc
      781 tggggcccgc acccgcggag cccgcctgac ggcaccgggc aggcgggcgg cggacggcgc
      841 tgcgcacccc gtcgcagccg accacccacc gtgcggtgac gtggctgcgg gtcccgtccg
      901 cgctccggag gtccacgcgg acgcgctcgt cgtggacggc ggtgcactcc tggccgcgca
      961 ggatcctcgc gcccagtccg ccggcccgct cggcgaagac gtgctcggcg taggcctggg
     1021 ggctgccggc gatgacgggg ccctccgcgc tcacctcggc gaggtcgagg tcgaacatcc
     1081 ccgcgaagtg gaaggggacg ctggtccgtc ccccgcgcgc cggtcgcggc tgcaccgcct
     1141 cgcgcagccc gcggcggtgc acaggctgcc gtcgtcctcg cgcaggccga tgcacgcgag
     1201 gtccgcgttg acgatccgtt tgacccgccg ggtgatgacg tacagcagct cgtccgggcc
     1261 gtacgggcgg atcatgtcgc tggcggtgtc cacgagttcg gcgagcgcgt cctcgcgctg
     1321 gcggtgccgg tcctgggagg cgtgtatgac gtaggccagt ctgacggcct gttcgaggcg
     1381 gagccgctcg gcggcggagc attcctggcc ggcgcgcagc aggagttcct cgtagctctc
     1441 cagcggcgcc tcctcggcga ggagtctgag cacggtgagc aggtcgaggc gatgtgtgtc
     1501 cgctccgtcc accatgccct gccccccgtc ggctgcgcgg ctccgtcgaa actaggcaga
     1561 gccggattcg ccagtcaaac ccctcttccg ggcgtgcgcc gagcgcccgt tcggccgccg
     1621 cgggcccggc gtgcggcggt gcccgggacc gtgccgtcac cgcttccgca ggcggcaccg
     1681 gccggtccgt cacgtcgcgc gggcggggcc ggcgcggagg gcccacccgc cggtcagatc
     1741 ctctggagtc gcgggagtcc gggtgggggc gcggcgctgc gggccaggtc gccgccgcgc
     1801 ggggtccgcg gtgccaccct gtccggcacg ccccggcggg cggtgagcgc cggggagccg
     1861 cggcgtcgac gcagataccg cctgatcacc tcggggtcct ggccgtcgtg gtccagatcc
     1921 gccttcagct gggtgtagag cggcgccggt gagtggaacc gggcgagcac cccggcgagg
     1981 tccgcgcaga gttcgcgccg gcgtccgagc cggaagtccg cctcgatcca ccggtcgacg
     2041 gcgcgctgcc aggagtgggg gagcagcacc gcgtgggagc gcaggtgcgg tcccttggcg
     2101 actccgtgaa cggggccgcc gtccacaggt ccaccgcctc acgcagccgg cggcccgcca
     2161 ggtgcaggtc tcctgcctcc agcgcccggt agcccgctcc ggccgtccgc tcgaacgccc
     2221 tggcgtcgct ggagccgccg ccggtgtcca gccggtacgc accgggcacc cggaccagca
     2281 cgtcctcggc cgaccggtcg ctgccggccg cctgcaacgc cgcgccgatg tcggcacgca
     2341 gctgacgggt atgggattcc atcgcctgtt cggcgtgcgc gggcgccccc tgcggccaca
     2401 gttcctcggc gatggcgcac gtgggtacga cgcggtcggc ggatgccgcc agcagggcga
     2461 gcacctgcct gggcaggacc gcggacggcg agatcggcac tcccccctcg tgcgccgcca
     2521 gtcctccgag caccctcagg tccatgtcgc cgtccccccc ctcagccggc tcgacgattg
     2581 ccgacccgac taacaataac atggtaattt ttttgttttg gaaccctccg gagttcacga
     2641 acagggctaa tctggcgggg cggtcaccac cagcggcatt gcgtctgcga agctccgggg
     2701 agaggggacg atgaggcagg agagagccag tcgcacccgt cgcgccctgc tgagggctgc
     2761 cgcggaggag ttcgaccgcc tcggttacgc gggagcgtca ctcgccggca tcgcgcgggc
     2821 cgcgggaatc tccctcgggg cgctgacttt ccacttctcc tccaagcagg agttgtcgac
     2881 ggccgtcagc gaacaggggt gcgccgcgac ccgcgccctc gtggcccgca ccggtgcgcg
     2941 cggggaacct ccggtgcagt gcgccgtgtc gctcacgctc ggcctggtgg aactcctgga
     3001 gaaggacgca tcggtgcggg ccgcggcgcg gttggaccgc gaggccgccg atgccgggca
     3061 cgggtggcag ggtgtctggg caccggtgat ccacgagtgc ctgcgggcgg cggccgacgc
     3121 ggaggggagc ccggtcgggg actcggccgc gctcgcggcc ctggcggtgc tgctggtctg
     3181 cgggaccgag gcgaccctcc cccgccccgc cggccccggc agccctgccg gccctgccgg
     3241 ccacggcggc cagcagggca actcggccct gctggcccgc atctggcggc agatcctgcc
     3301 tcagacggcc caggccgggt gcggccccgg gcggccgccc cagccccgcg gttctcacac
     3361 ggcgctgcat cctccgtcca cgggcacccc ggcccccgtg acgaacgagg cgtgatcgct
     3421 gcagagccag agcgccgcct cggccacttc ccccgggtcg gcgagacggt gctggacggc
     3481 ccgcgcgacg gccgcctgct ccagccgggg aacctgctcc atcgccgtct ccatcaactc
     3541 ggtgcgcgtg ctgccgacca tcagcgcgtt gacgcggatg ccgcggtcgc cgtactcggc
     3601 ggccgcggcc ctggtgagac cgagcaccgc gtgcttggcc gccacgtacg gggcgatggc
     3661 accggtcgcg aacgaccccg ccgtgctcga cgtgttgacg atcgaccccg ccccctgccg
     3721 cagcatgacc gggacctgct ccttgaggca gttccacaca ccccgcacgt tcacgtccat
     3781 gatctgctcg aagacggcgt cgtccgtctc gtgcagcggt gaaccgacac tcccccaacc
     3841 cgcgttgttg aaggccgcat cgagccggcc gtacatcgcg accgccgcgg tcacggcccg
     3901 ccgcacgtcc gccggcgacg tcacgtcccc ggccgtcacc agggcactgc cgccggcggc
     3961 cgagatgtcc tccgccagcg cgtgcagcct gtcctcgcgc cgcgccatca cggtcaccgc
     4021 cgccccctgg gccgcgaaca gccgagccgc ggcctcacct atgccactgg acgctccggt
     4081 gaccatgacg gtcttgcccc ggagcaggcc ccgcgcggac tccaccacgg tgttcccctc
     4141 tcgccttgac gatcacatga cgtccagacc gtaagggtgc cgccgggcgg agcccaagac
     4201 ggagcccaag gccgagccca aggcggtgcc cggtccgtca cacgcccttc gcccgggacg
     4261 cgccgaaccg cagtcccttc acggcgctcg gcaccgcgat cgccggaagc aggcaccgcc
     4321 acatctcctc cacccggccc ggcaggtccc ggcgccgggt ctccagatgg gaatgcatct
     4381 gcgcccccgt gcacgcgttg accaccagcc gggccagcgc ctccacatcg actcccgccc
     4441 gcagctcccg ccgccgcttc gcctcgtgca ggtcctcgga gaccaaacgc gtccactgct
     4501 ggtgggagtt ctgcgagggc ccggcgaagt gctcctgttc catcaccagc cgcgccccgg
     4561 cgagcagcag cgggtcgtcg agcagctggt acgcccaggt gagggtcacg tcgacggcgt
     4621 gctgcagtcc acggcagggg tgcgggggca ccacgaggtc cggctgggag agcacgatct
     4681 cgcgggcgag ctgccccttg ttgcggaagt ggaagtacag ggcgcccagc gtgaggccgg
     4741 cccgttccgc gatcgccgac acgttcgctc cggcatagcc ggactccgcg aacacctcgg
     4801 ctgcggctcg cagcagcgcg gccctcgtct gtacggcccg ctcctgcctg atcactcgac
     4861 ctgccatgac tccacccatc cctcaataaa gaaaattgca acctttatct ttacccaggg
     4921 ctcactggaa cacctgatcg actccggatc tcgtggtgac aggcccctct gctcgccgac
     4981 gtcccaaggg ggaggacgca atgcacagca gcgcacacac cgacttacgc accagcgatc
     5041 ttcagcggct cgcccgcaag gcgagccccg gagaggtctt cgtccgggac tggcgcgagg
     5101 agtccccgca gacccatgtc gtcgctctcg gctggccgcg ttcccacacc ttctacacgc
     5161 tccgcagcgg agccaccagc ccgctgctct tcgtcgaggc cgtccggcag gcactcgcgg
     5221 tcctgtcgca cacggcccag gagatacccc tcgaccaccg cctcggacgg gactccgccc
     5281 gctgcacctt cgccccggcg gccttccggg agcgccgggc gcccaccgat gtggaactgc
     5341 gcgtcaggca cagcggcgtc acccggcgcc ggctcggctc ggtccggctc accgcccgca
     5401 tcgaggccac ctgcggcggc gaggtcctgg gctgcgccga gatcgggtac gccacccacc
     5461 cgcccgtcat ctacgaccgc ctgcgcggga cgtacgccga cgcacagcag tccttcgccg
     5521 cagcgctccc gccggcacgg cccgtcactc cggcgctggt cgaccgcacc cacccggggg
     5581 acgtcgtcat cgcgtgcacc tccaaggcgc accactacca actgcgcacg gacacctcca
     5641 actccgtgct cttcgaccac ccgcacgacc acgtgccggg catagtgctg ctcgaagcag
     5701 tggtccaggc cgtgcacgcc tcggcaccgg gacacgcgcg actcgcctcc ctcgacaccg
     5761 ccttcctgcg gtacgtcgag ttcgacatgc cctgctgggt cgacgtcgcg cccgggccgc
     5821 cggacgcgca gggcggttcc cggctcctgg tgaccggcac ccagaacggc cggaaggcct
     5881 tctccgcgac cttggccttc gactccccga ccacctccct cgagcccgcg gtgcccgccg
     5941 gcccggaacg cctcggcacc gggcccactc cggcggcctg cccggccggg tagaacgccc
     6001 ggcggcgccc ggccggcgag ccaccgcacg atctcccgga gtccacccga ccccggagat
     6061 cacgcggtgg cctcaccccg ttccggcagt gcccgccgca cgatcgggag gcggggccgg
     6121 ggcgtcagtt gtagcggccg aagacctggg tgcgggtgaa ccagccaccg gcgggggaat
     6181 gccatccggc tgccggctcg ttgaaggtgc cgtccggttt cacggtccag gtgaacatct
     6241 tgacggtgct gtccgcgtag ccgtacacgg tggccaggtc gtcgcggccg tcgccgtcga
     6301 agtcaccggc caggagcttc atcgacgagc gcgtgaagcg tccggcgacc gcgttccagg
     6361 cggtcctggg ggcctggaag gttccgtcct ccgtactggc gccggcgaag acatggatgg
     6421 cgtcgctgcc gtcggcgtag tcgtaccagg tggcgacgtc gtcgcggccg tcgccgtcga
     6481 agtcacccgc gtggaagtcg gtgcgggccc agtctcccca ggaggtgctg gtccacgact
     6541 ccacggggtt gccgaagccg ccggcggcac tggccgggaa gctccagatc ttgacgctgc
     6601 cgtccgagta ccggtacagc acggcgagat cgtcgcgacc gtcggcgtcg aagtcaccga
     6661 cggcgaactt gcagttggtc acgtcccagc tggaagtgct ccaggagacg gacggggcgg
     6721 tgaagccacc ctgggcgtcg gccgggaacg tccacagttt gttgccctcg gtgtagcggt
     6781 accaggcggc gatgtcgtcc cggccgtcgc cgtcgaagtc gccgctgtgc agggtcatac
     6841 ggtccagctg ccagtcgccc tcccatgacg agaagggggc gttgaagcgg ccgtcgctcg
     6901 tggcggtcca cgtccacagc ttgacccccg cggtgtactg gtaggcgacg gcgaggtcac
     6961 ctcgcccgtc accgttgtag tcaccggtgg tgatcttcat gagcgagggg tcccagcccg
     7021 ggacggtccg gctccacgcg gtgaacggcg ccttgagcgt cccgtcctgg tcggcgagga
     7081 aggtgtggac ggcgtcgtgc tggtcggggt attcgtacca ggatgccagg tcgctgcggc
     7141 cgtcgccgtt gtagtcgtgc ctgacggccg tgccgctgga ccactgcgac cggtcctggg
     7201 cgtcgtagac gacgaggttg ccgcggtcct gcaggcggac atacccgccg ggggcggtgg
     7261 tgccggtctg ccacagcacg gttccgtctg aggcgactac ctggagggca ccggtgccgg
     7321 tgaaccgggc ggtggcgccc gggttgccgc ccgtgccggt ggaccacagc accttgcccg
     7381 cgctggaggc gatgacgagg tcgccgtcgg tctgcatggt caggcggttg gtggcggagg
     7441 cgagcatgtc gccgggcagc agttgctgcc cggtgccgag gcggctgccg aggctggtgc
     7501 tgtcggcccg cgcggcgatc gcgtcggtac gggtctcggc cgcgtcctgg ccgaagcagc
     7561 cgccctgcca ggagcggctg gcaacggcga cgagttcggg ggtcccgttg ttgtcgcgca
     7621 ggagcgggcc gccggtgtcg cccttgcaga tcgcgtcacc ggctgtcttg ccaacgatgt
     7681 caaggccggt gtccgtaacc gcgccgacgg tgaagccggc ggtgtggagc ttgtcgggca
     7741 cccactccgt cttcgtccgg ccgtatccgg ccaccgtcac cgtctcgccc tgaccggccg
     7801 gagtcgcgga gatcttcacc ggggtgatac cggcagcggg tgccgccagg cgcgccatga
     7861 ccaggtcacg gcccgcgcgg ggcaccaggt cgacgatctc gctgacatga ccgccggttg
     7921 aggtgagatc ggcccggccg atcgtcgcca ccgtcttctc ggcaggcttg ccgggggcga
     7981 gttcggccag cccaccggtg aagcacgaag cagccgtcag cacccaacgc gcatcgacca
     8041 gggcacccga acaggcacgc ctgtcctccc cctcaccgat ctccaccctc gcggtgaacg
     8101 cgtaggagct gccggcgacg gcgggaccgg tcaccgcctg agcgggtacg gcggccagcg
     8161 cccatggcgt ggcggcgagg gctgtggtga acgcggcgag gcgacgggat ctggagtgtc
     8221 gcatgtccct gcttcctggg gtggtcggcg tgtctggtgg ccactggagc gaagtacggc
     8281 ggagatcggg gcgctgaact gctacttggt ggacctgatc gctacgagca tatattcgag
     8341 cccctgctcg tcagcgctct cacccacccc ggtccaggtg ttctcggcag tggcgaaaga
     8401 cccctcctcg gagccgacgg ccatacggac ccgcgtggtg cagtcgtcgc ccctgaccgc
     8461 gtggaccgcg aggatctcca ctcgctcaga gctgccggca ggggacgaga agggagacgt
     8521 tgcggcaacg gcgtccgtcg cccgcggggc gttcggctgg atgacattcc gttgagagat
     8581 cgcatgcggg ccccggccca cccaggcggc gtgatgtgcc cgaggtggac ggtgagcgct
     8641 gcggccgttg tgtcatcatg cggagcgccc tgggtgggga agagggccaa cgcgtgtgga
     8701 gaggcgagga gttcggtgtg aatgcgggtg gggagcccag cggttcgtca gtacctgacg
     8761 aggaatggga acggtttctc cgggattcga tgaccggtgc ggcgaacgct cccagggaac
     8821 cgtcggcacg ggcccgggcc gtggcgaggc ggatgcgcga ggagcccgcc ggcgcggagg
     8881 ggtggcggac gtactcgttg gcccgtcccg gtcccagccg gaagaagcgc tggtacatgg
     8941 tcgggctctt ggctaccacg gtggccctgg tgatcctggc gctggatccg ggacgggtgg
     9001 tcggatggtt cagcgacagc aaccgggccg agaagccgct cggcccggaa tcgcatcgcc
     9061 cgatccaggc accttccgcg gagggcttcc gacagcccac gctcgacgaa ccgttcagag
     9121 actcgcccgc ggctcgctgg gcgaacggca cggccgggat cacggtaccc gaggccaggg
     9181 cgaccggctg gatgagtcgg gcgcaggtgg agcgggcgct ggagcagacc cgggacttcc
     9241 tcgctgcggc gaacctggac ccaggggcgc tgcacggcgg acatcccgaa gaggcgatcg
     9301 cgctgatcaa ccctcaccaa caggacgtcc agacctatct gaaggccgca ttccactcgc
     9361 cgagcgagca gaacgacccg ctgctgctct tcagccggtt cgacgcctcg cgcgtgcggc
     9421 tcgtcgggga cgtggtgaag acccggggcc ggatcacgta cctggagggc gaacgcggcg
     9481 cactgcaggt gaccagcgac gtcacctacg tgtacccggt cgcgccggca acgggcggcg
     9541 acgaggtcgt gcgtacgatc gtgcggcgcg aggtggtgct gagctgggac gaccccgcga
     9601 aggtgatcac cgagccggga accttctccc tggtctcgta caaggtcgac atgaccaacg
     9661 ggggctgcga caactccacc ggctacctca ccccgccctt cggcacggaa agggcggaaa
     9721 ccggcgcggg ccccgacgtc gacccgtacg accgcagcac ttcgatgagc gagcggacga
     9781 ccccggacga aggatgcggc accgcgacac ggtcgtagga cgtcttccgg ccattccgca
     9841 tcaccgccac accaggcccc tggcgtccgc cggccccgca cgccagggac tcctcagggg
     9901 ccttcggcgc tgcccgagga cttccgaggg acccttcgcg gccgggagtg tcccagatct
     9961 agcgtcgtac ctgaatcagc gcgtgagttc cactcgtacg ccaggcagcc ccggaccgtt
    10021 cgagcgtgtc cagcgctccc tgctccagac cgacgatgac gtcggacttc agggtccgga
    10081 gcgccgcgac ggcccccggg aagtatgcgg tgagatccgc gaagggcgcc gtggggtgcc
    10141 acagctggtc gcccaccaga cggcggtagt tgaggtcgcc cttcaggatg gtgagcgtgg
    10201 cgctcgcgaa cccgacacga agatcctcgg gcatctcctc atacggcaga ggagcgcaga
    10261 agaacgagtg ggtgcggacc tccagggttc cggccgccat ggccttccag agtcggccgc
    10321 cgatccgacc ggcttcgccg ggcgcctggg tgaggcggcg aaggcagtcg accacgtccg
    10381 ccgtcatcgc gtcggagacg tagtacgggt agggcttgac gtaaagcgca acccgctcgg
    10441 catgcccgtg ttcgaggagg tggtcgatga gaacgaggtc gggaatcagc tcacgtcccg
    10501 cgttgtcggc caccacggct acggtggccg aggtgccggc gggcaggagc tgccacagcg
    10561 tggcactgtc gtcggcgacc agcgcagagg tcgcggcgtc actcgttccc gactcccctg
    10621 ccgagatgct gaagccgagg tccgcgcggt tgccccaaag cgacgcgtgc agcagcgccg
    10681 ttgcccgctc atgggacgga gcgtaggcga gcgcgtccag ggcccgcagc tcctcctcca
    10741 ccgcctcgcc tcgcagttcg gcccgcttga acggcgcgaa cggatccact ccctgccacg
    10801 gcccgctgcc gatgtacccg accgcgctga gcagcctgcg gtagaagtag ctctccgccc
    10861 acaggaacgg ggcgtcgtac cacgagcgcc cgaagtactc ccgcccccag tccgcccagc
    10921 gctcgtagtc gcgctccgag gggccaagca gctcgatcac accgttggtg atctcgtcca
    10981 gcagggcgtc aag
//
