LOCUS       NZ_CP029254            10429 bp    DNA     linear   CON 23-OCT-2024
DEFINITION  Streptomyces spongiicola strain HNM0071 chromosome, complete genome.
ACCESSION   NZ_CP029254
VERSION     NZ_CP029254.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces spongiicola
  ORGANISM  Streptomyces spongiicola
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7180417)
  AUTHORS   Zhou,S. and Huang,X.
  TITLE     Complete genome sequence of the Type Strain of Streptomyces
            spongiicola HNM0071, the producer of staurosporine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7180417)
  AUTHORS   Zhou,S. and Huang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2018) Institute of Tropical Agriculture and
            Forestry, Hainan University, No 58, Haikou, Hainan 570228, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 850.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003122365.1-RS_2024_10_23
            Annotation Date                   :: 10/23/2024 01:04:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,008
            CDSs (total)                      :: 5,921
            Genes (coding)                    :: 5,739
            CDSs (with protein)               :: 5,739
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 47 of 182
            Pseudo Genes (incomplete)         :: 161 of 182
            Pseudo Genes (internal stop)      :: 9 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:10:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 497795
            Orig. end                         :: 508224
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029254.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10429
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="4"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10429
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10429
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5429](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5429
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(450..461)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            complement(1209..2297)
                     /locus_tag="DDQ41_RS02120"
                     /old_locus_tag="DDQ41_02120"
     CDS             complement(1209..2297)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989359.1"
                     /locus_tag="DDQ41_RS02120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_02120"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_109292919.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 8.2e-18, bitscore:
                     63.1, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDHAVLHVTGRVLVGPDDVRDELWVVGGRVTYRRPATGDVRTVR
                     GWALPGLVDAHCHVGLDRHGAVDEATSEKQALTDRDAGTLLIRDAGSAADTRWIDDRED
                     LPRIIRAGRHIARTRRYIRNYAHEIEPEDLVAYVAREARRGDGWVKLVGDWIDREVGDL
                     AACWPRGAVEAAIAEAHRLGARVTAHCFAEDSLRDLVEAGIDCIEHATGLTEDLIPLFA
                     ERGVAIVPTLVNIATFPQLAAGGESKFPRWSDHMRRLHERRYDTVRSAWDAGIPVYVGT
                     DAGGSLPHGLVAAEVAELVKAGLPAVDALSATTWRARDWLGRPGLEEGAPADLVVYESD
                     PRADVRVLAAPSAVVMRGRVVG"
     misc_feature    2652..2670
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.59"
                     /tool="antismash"
     misc_feature    complement(2674..2691)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.01"
                     /tool="antismash"
     misc_feature    2803..2818
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2951..2965
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.34"
                     /tool="antismash"
     gene            2974..3540
                     /gene="ectA"
                     /locus_tag="DDQ41_RS02125"
                     /old_locus_tag="DDQ41_02125"
     CDS             2974..3540
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986839.1"
                     /locus_tag="DDQ41_RS02125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_02125"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_262508332.1"
                     /transl_table=11
                     /translation="MTTALSIDSPSVEDGAAIWRIARDSEVLDLNSSYSYLLWCRDFAA
                     TSLVARGPDGEPIAFVTGYLRPERPDVLVVWQVAVDGEHRGRGLAGTLLDALTHRVVPR
                     GIRAVETTVTPDNTASDRLFASYAARHGASLDREVLFDGGLFPDGGHQPEVLYRIGPIG
                     PIGPMEPTEAVGPGASIGTAAPVGD"
     gene            3672..4940
                     /gene="ectB"
                     /locus_tag="DDQ41_RS02130"
                     /old_locus_tag="DDQ41_02130"
     CDS             3672..4940
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 1.5e-72.
                     Score: 236.4. Matches aSDomain:
                     nrpspksdomains_DDQ41_RS02130_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="DDQ41_RS02130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_02130"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_109292920.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.1e-76, bitscore:
                     255.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALLDYLERDGITHGLDMATTAKRAFLESFQNAVLRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGQVQDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALADLCRRQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTLSKSISGYGLPMA
                     LCLFNPELDVWEPGEHNGTFRGNNPAFVTAAAALDAYWADGQTEKQTIARGEQVEQALL
                     GICDENSALGAHYRGRGLVWGLEFTDKSRASAVCARAFELGLLLETSGPESEVVKLLPP
                     LTVTPDELDEGLRTLARAVRETA"
     aSDomain        3756..4766
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DDQ41_RS02130_Aminotran_3.1"
                     /evalue="1.50E-72"
                     /label="DDQ41_RS02130_Aminotran_3.1"
                     /locus_tag="DDQ41_RS02130"
                     /protein_end="365"
                     /protein_start="28"
                     /score="236.4"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHTYLDFFAGAGSLNYGHNNPVLKRALLDYLE
                     RDGITHGLDMATTAKRAFLESFQNAVLRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGQVQDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALADLCRRQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTLSKSISGYGLPMALCLFNPELDVWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWADGQTEKQTIARGEQVEQALLGICDENSALGAHYRGRGLVWGLEFT"
     misc_feature    complement(4984..4998)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.44"
                     /tool="antismash"
     gene            5001..5429
                     /locus_tag="DDQ41_RS02135"
                     /old_locus_tag="DDQ41_02135"
     CDS             5001..5429
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018890221.1"
                     /locus_tag="DDQ41_RS02135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_02135"
                     /product="ectoine synthase"
                     /protein_id="WP_109292921.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.9e-52, bitscore:
                     174.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLKDIENTDRHVRSKSGTWESKRIVLAKEKAGFSLHETVLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEAHRIEPGTMYLLDGNERHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGTYPAPGPEARGDHQHTEEG"
     gene            5432..6322
                     /gene="thpD"
                     /locus_tag="DDQ41_RS02140"
                     /old_locus_tag="DDQ41_02140"
     CDS             5432..6322
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="DDQ41_RS02140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_02140"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_109292922.1"
                     /sec_met_domain="PhyH (E-value: 6.6e-36, bitscore: 123.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAITDLYPTRGATETAVPRRDPVVWSAPGAPGPISAADLQGYE
                     HDGFLAMPELLTPAEVEVYRTELDRLVDDPLIRADERSIVEPRSQSVRSVFEVHKISEV
                     FGALVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMR
                     TVSVSVALTENHDTNGGLMIMPGSHKTFLGCAGETPKDNYRKSLQMQDAGTPSDEALTA
                     FADRHGIRLFTGRAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENAAREPFAAPV
                     PRPTFIGARDFTPVR"
     gene            complement(7005..8048)
                     /locus_tag="DDQ41_RS02150"
                     /old_locus_tag="DDQ41_02150"
     CDS             complement(7005..8048)
                     /NRPS_PKS="Domain: Aminotran_5 (47-340). E-value: 1.7e-23.
                     Score: 75.1. Matches aSDomain:
                     nrpspksdomains_DDQ41_RS02150_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948835.1"
                     /locus_tag="DDQ41_RS02150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_02150"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_109292924.1"
                     /sec_met_domain="Aminotran_5 (E-value: 5e-17, bitscore:
                     60.3, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2.1e-05, bitscore:
                     22.3, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.8e-06,
                     bitscore: 25.9, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METLGGAEFAPTSTYLNTSFCGLLPRRSVEAVGRLAREIADGRPE
                     GSGSTGAVEAARASFARLVGVSADRVALGGSVAVHVGLIAASLPAGAEVVVPEGEFSSV
                     VTPFTTRDLKVRYAPLDSLAEAVGPGTALVALSAAQSADGRIADLPAVRAAAAGHGARV
                     LLDASQAAGWLPLDAGRYDYTVTAGFKYLLCPRGASFLTVTEEAQESLVPVHAGLYAAE
                     DPAGSTYGPVEHFARSARRYDEPPSYLSYHGAAQSLPLLEEAGIATVHAHATALAARFR
                     DGLVRLGHEPVPGDSAIVGVPGLGERKPLLDRAGVVVSARAGILRVSFHFYNSAADVDR
                     ALDVLAG"
     aSDomain        complement(7029..7907)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DDQ41_RS02150_Aminotran_5.1"
                     /evalue="1.70E-23"
                     /label="DDQ41_RS02150_Aminotran_5.1"
                     /locus_tag="DDQ41_RS02150"
                     /protein_end="340"
                     /protein_start="47"
                     /score="75.1"
                     /tool="antismash"
                     /translation="GSTGAVEAARASFARLVGVSADRVALGGSVAVHVGLIAASLPAGA
                     EVVVPEGEFSSVVTPFTTRDLKVRYAPLDSLAEAVGPGTALVALSAAQSADGRIADLPA
                     VRAAAAGHGARVLLDASQAAGWLPLDAGRYDYTVTAGFKYLLCPRGASFLTVTEEAQES
                     LVPVHAGLYAAEDPAGSTYGPVEHFARSARRYDEPPSYLSYHGAAQSLPLLEEAGIATV
                     HAHATALAARFRDGLVRLGHEPVPGDSAIVGVPGLGERKPLLDRAGVVVSARAGILRVS
                     FHFYNSAADVDR"
     gene            8268..8738
                     /locus_tag="DDQ41_RS02155"
                     /old_locus_tag="DDQ41_02155"
     CDS             8268..8738
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436199.1"
                     /locus_tag="DDQ41_RS02155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DDQ41_02155"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_109292925.1"
                     /transl_table=11
                     /translation="MEVVLREVRRPDLDTFFALMQDPEAARMAAFTPGDAGDRARFDAR
                     WERVLRTDDVNRTVVADGVVAGHAAVYGPPGEREVTYWIDRALWGRGVATSALRALLLL
                     VPERPLHARAAADNAGSLRVLAKCGFTVAGRGTGFAHARGGETDELLLRLDR"
     misc_feature    complement(9155..9169)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.85"
                     /tool="antismash"
ORIGIN
        1 atctcgcggt caacgtccgc accggcaccc ctccggtgtg gctgcgggag cgcatcgcgg
       61 cgtcactggc gtcgctcgcc gcctacccgg acggccgtgc ggcacgggcg gcggtcgcgg
      121 cgcggcacgg ggtgccggtg cagcgggtgc tgttgaccgc gggcgcggcg gaggccttcg
      181 tgctgatcgc gcgggccctg cgggtgtacc gcccggtggt ggtgcacccc cagttcacgg
      241 agcccgaggc ggcgctgcgg gacgcggggt acgaggtggg ccgcgtggtg ctgcgcgcgg
      301 aggacggctt ccggctggac ccggcggccg tgcccgagtc cgcggacctg gtcgtcgtcg
      361 gcaaccccac caacccgacc tcggtcctcc atcctgccgc cgagttggcc cggctcgccc
      421 ggcccgggcg gatcctggtc gtcgacgagg cgttcatgga cgcggttccc ggtgaaccgg
      481 aaaccctcgc cccgaggacc gatgtgcccg gactgatcgt ggttcgcagc ctcaccaaga
      541 catgggggct cgcggggctg cggatcggct acgtcctcgc cgggccgggg acggtcgccc
      601 ggctcgggcg ggcccagccg ctctggccgg tggggacccc cgcgctcgcc gcggccgagg
      661 cgtgcatgtc tccgggggcg ctcgcggagg ccgcccgcgc ggcggagcgg atcgcggcgg
      721 accgggccca tctgctggcc ggtctcgcgg agttcgacgg ggtacgggcg gtgccggcgg
      781 cggcgggccc gttcgtcctc gtccgcatcg accgcgcgga cgaggtacgc ggacggctgc
      841 gggcgctcgg cttcgccgcc cggcgcgggg acaccttccc ggggctggac cgcgactggc
      901 tgcggctcgc ggtccgcgac cgggccacga cgaaccgctt cctccagggc ctcgaccagg
      961 ccctgaggtt cgtcgcggtg taggcgccac gctggggggc gtccagtgaa gtgctcggcg
     1021 acgcgcccag tcgcgtacgc agtgccgcgc ccagtgacgt acgcagtgcc gcgcgcagtg
     1081 acgtacgcag tgacgtacgc agtgaagtgg cgcgtccacg cggggagacg cggcgggggt
     1141 gcgcgcgagc gggcggcggc tgcgaggtgg cggggcgaag ggaaccgtcc ggtcggggtc
     1201 gcggtccgtc agccgacgac gcgcccccgc atgaccaccg cgctcggcgc cgccagcacc
     1261 cgtacgtccg ccctcgggtc gctctcgtac acgaccagat ccgccggggc tccctcctcc
     1321 aggcccggcc gccccagcca gtcccgcgcg cgccaggtgg tcgcggagag cgcgtccacc
     1381 gccgggagcc cggccttgac cagttcggcg acctctgcgg ccacgagccc gtgcggcaga
     1441 gagccgccgg cgtcggtgcc gacgtagacg gggatgcccg cgtcccaggc cgagcggacg
     1501 gtgtcgtaac ggcgttcgtg gagccgtcgc atatggtccg accagcgggg gaacttgctc
     1561 tcgccaccgg ccgcgagctg cgggaaggtg gcgatgttga ccagcgtcgg gacgatggcc
     1621 acaccgcgct cggcgaacag cgggatcagg tcctcggtca gtccggtggc gtgctcgatg
     1681 cagtcgatgc ccgcctcgac gaggtcgcgc agcgagtcct cggcgaagca gtgggcggtg
     1741 acccgggcgc cgaggcggtg cgcctcggcg atggcggcct cgaccgcgcc gcgcggccag
     1801 caggcggcga ggtcgcccac ctcacggtcg atccagtcgc cgacgagctt gacccagccg
     1861 tcgccgcgcc gggcctcgcg ggccacgtag gcgacgaggt cctcgggctc gatctcatgg
     1921 gcgtagttgc ggatgtagcg gcgggtgcgc gcgatgtggc gcccggcacg gatgatcctc
     1981 ggcaggtcct cgcggtcgtc gatccagcgg gtgtcggcgg cggatccggc gtcgcggatg
     2041 agcagggtgc ccgcgtcgcg gtcggtcagc gcctgcttct cgctggtcgc ctcgtccacc
     2101 gcgccgtgcc ggtcgagccc gacgtggcag tgcgcgtcga ccagaccggg gagcgcccag
     2161 ccccggacgg tgcggacgtc cccggtggcc gggcgccggt aggtgacgcg cccgccgacc
     2221 acccacagct cgtcccgtac gtcgtccggc ccgacgagca cccgccctgt cacgtgcagc
     2281 accgcgtgat cgctcatggg gagcactcta cgagtcccgc cccggtggcc gtcccgcgac
     2341 cggggacgcc cggcgcagcg gggacacggg gaacgccgag taccccgagt acgccgagta
     2401 cgccgagcca ccgggcacgc tgagcacccc gggcaccccg agtacgccgc ggacgccgcg
     2461 gacaacgacg gcaccgcggg caccgacggc accgcgggca ccgacggcac cgggaacccc
     2521 ggcgcgcgtc agcggtgccg ttccgcactg cggatgccgc cggccccacg ccgtggaaac
     2581 cgccggcgcc ggtcgcgcgg acttccgggg gcgcgccttc gagggctcgg cagaaccaat
     2641 tccccgaacc gcaccgaaaa aacaaacagc gcattaatag gagaattacg tgagccacct
     2701 tttacaagag ctgcctcccg agttcgtctg cccgctttcc ggagcgctaa acgcatcggt
     2761 tcccaagaga tcccaagagc cattccgcgg gccccaccgc catgtgacgg acaccacaca
     2821 aacggcgttc tgtccgataa ctcccggtca aaaacggata attcgcccca ccctcacgcc
     2881 agggtgcgcg cccgcgcgat aacatatagc gcgggactcg ccaacccctc cccacgatgc
     2941 aatttatgaa tttgctcggt aaattaaatc cgcatgacca ccgcattgag catcgacagc
     3001 ccgagcgtgg aggacggagc cgccatctgg cgcatagccc gcgactccga ggtcctggac
     3061 ctcaactcct cgtacagcta tctgctgtgg tgccgcgatt tcgccgcgac ctccctggtg
     3121 gcccgcggcc cggacggcga gccgatcgcc ttcgtcaccg gttacctccg gcccgagcgt
     3181 ccggacgtgc tggtcgtatg gcaggtcgcc gtcgacggcg agcaccgcgg ccgcggactg
     3241 gccggcacgc tcctcgacgc actcacacac cgggtcgtcc cgcgcggcat ccgcgcggtc
     3301 gagacgaccg tcaccccgga caacaccgcc tcggaccgcc tcttcgcctc gtacgcggcc
     3361 cggcacggcg cgtcactgga ccgcgaggtc ctgttcgacg gcgggctctt ccccgacggg
     3421 ggacaccagc ccgaggtgct gtaccgcatc ggacccatcg gacccatcgg acccatggaa
     3481 cccacggaag ccgtcggccc cggcgcctcg atcggcaccg ccgcccccgt cggcgactga
     3541 gaccggcgct ccggccggtc ccggacaccc gttccgcgcc gggccgcccc gggcaggccc
     3601 gcgcgacccc gcacgacccc gcacgacccg catcccagct ccgtccgacc ccacatcagg
     3661 agtgaaacgc tgtgaccatc accccgcccg ccctgagtgt cttcgagacc ctggagtcgg
     3721 aggtgcgcag ctactgccgc ggttggcccg ccgtcttcga ccgcgcgcag ggcgcccgcc
     3781 tcaccgacga ggacggccac acctatctcg acttcttcgc cggcgcaggg tcgctcaact
     3841 acggccacaa caaccccgta ctcaaacgcg cgctgctgga ctacctggaa cgcgacggca
     3901 tcacccacgg cctggacatg gcgacgaccg ccaagcgcgc cttcctggag agcttccaga
     3961 acgccgtact gaggccgcgt gacctgccct acaaggtgat gttccccggc ccgaccggca
     4021 ccaacgccgt cgaggccgcg ctgaagctgg cccggaaggt gaagggccgc gagtcgatcg
     4081 tctccttcac caacgccttc cacgggatgt cgctcggctc cctcgccgtg accggcaacg
     4141 ccttcaagcg cgccggcgcc ggcatcccgc tggtccacgg caccccgatg ccgttcgaca
     4201 actacctgga cggccaggtc caggacttcc tctggttcga gcgcctgctc gaggaccagg
     4261 ggtccgggct gaacaagccc gccgcggtca tcgtcgagac cgtgcagggc gagggcggca
     4321 tcaacgtcgc ccgccccgag tggctgcggg cgctggccga cctgtgccgg cgtcaggaca
     4381 tgctgctcat cgtcgacgac atccagatgg gctgcggccg cacgggtgcc ttcttctcct
     4441 tcgaggaggc gggcatcacc ccggacatcg tcaccctgtc gaagtccatc agcggctacg
     4501 gcctgcccat ggcgctctgc ctgttcaacc cggaactgga cgtctgggag ccgggcgagc
     4561 acaacggcac gttccgcggc aacaacccgg ccttcgtcac ggcggccgcc gcgctggacg
     4621 cctactgggc cgacgggcag acggagaagc agaccatcgc ccgcggcgag caggtggagc
     4681 aggccctcct gggcatctgc gacgagaact ccgccctcgg cgcccactac cgcggccgcg
     4741 gtctggtctg gggcctggag ttcaccgaca agagccgtgc gtcggcggtc tgcgcgcggg
     4801 cgttcgagct gggcctgctg ctggagacct cgggcccgga gagcgaggtg gtgaagctgc
     4861 tgccgccgct caccgtgacg cccgacgagc tggacgaggg ccttcgcacg ctggcccgtg
     4921 ccgtccgcga gaccgcctga ggcccccgcg gcaccccgac ccaccccgta cggcgaggaa
     4981 agaaaggttc tgaaagcacc gtgatcgtcc gatcgctcaa ggacatcgag aacaccgacc
     5041 ggcacgtccg gtcgaagtcc ggcacctggg agagcaagcg gatcgtgctc gccaaggaga
     5101 aggcgggctt ctccctccac gagaccgtgc tgtacgccgg tacggagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtgctctgcg tggagggcga ggcggagctg acgaacgacg
     5221 agaccggtga ggcgcaccgg atcgagcccg gcacgatgta cctgctggac ggcaacgagc
     5281 gccacaccct gcgtcccaag acggacttcc gctgcgtctg cgtcttcaac ccgcccgtca
     5341 ccgggcgcga ggaccacgac gagaacggca cctaccccgc ccccggaccc gaggcccggg
     5401 gggaccacca gcacaccgag gagggctgac catgaccacc gcgatcaccg acctctaccc
     5461 gacccgcggc gccaccgaga ccgccgtccc ccgccgggac ccggtggtgt ggtcggcgcc
     5521 cggagcgccg ggcccgatct ccgcagccga cctccagggg tacgagcacg acggcttcct
     5581 cgccatgccg gagctgctca ccccggccga ggtggaggtc taccgcaccg agctggaccg
     5641 gctggtcgac gacccgctga tccgcgccga cgagcgctcg atcgtcgagc cgaggtcgca
     5701 gtcggtccgt tcggtcttcg aggtccacaa gatcagtgag gtcttcgggg ccctggtacg
     5761 cgacgagcgc gtcgtgggcc gggcgcggca gatcctcggc tccgacgtct acgtccacca
     5821 gtcgaggatc aacgtcaagc ccggattcgg ggcctcgggc ttctactggc actcggactt
     5881 cgagacctgg cacgccgagg acgggctgcc gaacatgcgg accgtgtcgg tgtcggtcgc
     5941 cctcaccgag aaccacgaca ccaacggcgg gctgatgatc atgcccggct cgcacaagac
     6001 gttcctgggc tgcgccggtg agacgcccaa ggacaactac cggaagtcgc tgcagatgca
     6061 ggacgccggc accccgtcgg acgaggcgct gaccgcgttc gcggaccggc acggcatccg
     6121 gctgttcacc ggccgggccg gctcggcgac ctggttcgac tgcaacgcga tgcacggttc
     6181 cggggacaac atcaccccgt acgcgcgcag caatgtcttc atcgtgttca acagcgtgga
     6241 gaacgccgcg cgggagccgt tcgcggcacc ggtgccgcgc ccgacgttca tcggcgcgcg
     6301 ggacttcacg ccggtgaggt gaactcgggg ccggtcccgg gggacgcccc gggacacggc
     6361 gtcggagcgc ggtggtccca cctcggtggc gccgccgtga gatgcgggca gggggccggc
     6421 cgctcgtgcg gccggcccct tccccgtgcg cggaccggtg ccgggagccc ttccgcccgg
     6481 accgggcagc cctcgccgcc ggcggagggc cggatgccct ccgtgatgac gagcccggcc
     6541 gaggcgccgc tgcgtgtggt ggtcgacggt ggagccggtc ggatgaggcc cgggccgaag
     6601 gcgcggctgc gggtcatggg cgccgtggcg atgcggctgg cgagcggtgt gccgcggagg
     6661 tcgaacgggt cgaaggcggt ggtcatgaca gctcccacgg agacgggcgg agaacactgc
     6721 cggtcggccg ggcatcggcc cggcggtcac ccggcccggc ccggcccggg aacacacccc
     6781 gcggggcgga ggggaatcgc acccggccct gagccgggcc gtcacggccg caccggacac
     6841 ccgccccggc cccgggcggc ggcagtccga ccgccccgtg cccggcagga cctcacccgg
     6901 cccggaaggt cccgcacccg gccccagggg ggcacgccgg gctgccccgc cgcgagcctc
     6961 ccccgccgcc gggcgcgccc gtcccggccg ccgggcgcgc ccggtcagcc cgcgagcacg
     7021 tcgagggccc ggtcgacatc ggcggccgag ttgtagaagt ggaacgagac ccgcagaatc
     7081 ccggcgcggg ccgacacgac gactcccgcg cggtcgagca ggggcttgcg ctcgccgagt
     7141 ccgggtaccc cgacgatcgc cgagtcgccg ggcacgggct cgtgcccgag ccgcaccagc
     7201 ccgtcccgga accgggccgc gagcgcggtg gcgtgggcgt gcaccgtcgc gatgcccgcc
     7261 tcctccagca gcggcaggga ctgcgcggcc ccgtggtagg agaggtacga gggcggctcg
     7321 tcgtaccgcc gggcggagcg ggcgaagtgc tcgacgggtc cgtaggtgct ccccgccggg
     7381 tcctcggccg cgtacagacc cgcgtgcacc gggaccagcg actcctgcgc ctcctcggtg
     7441 acggtgagga aggacgcgcc gcgtgggcac agcaggtact tgaacccggc cgtgaccgtg
     7501 tagtcgtacc gccccgcgtc cagcggcagc cagcccgcgg cctggctcgc gtccagcagg
     7561 acgcgcgccc cgtgcccggc cgcggcggcc cgcaccgccg ggagatccgc gatccggccg
     7621 tcggcggact gcgcagcgga cagggccacc agcgcggtgc cgggccccac cgcctcggcc
     7681 agcgagtcga gcggcgcgta ccgcaccttc aggtcccggg tggtgaaggg ggtgaccacg
     7741 gagctgaact cgccctcggg cacgacgact tccgccccgg cgggcaggga ggccgcgatc
     7801 agccccacat ggaccgcgac cgagccgccg agcgcgaccc ggtccgcgga cacccccacc
     7861 agccgggcga aggacgccct ggcggcctcg acggccccgg tgctccccga cccctccggg
     7921 cgcccgtcgg cgatctcccg ggcgagcctt ccgaccgctt ccaccgagcg tctcggcagc
     7981 aggccgcaga aggaggtgtt cagatacgtc gacgtgggcg cgaactcggc gcctcccagg
     8041 gtctccatgc gcccccaccc taacggggcc ggcctccccg gccgcggcct cgaccgaccc
     8101 gctccagcgg cttcccggtt cgaaggcccg tactccacac tggtgccgca cccgccgcgg
     8161 cctcctcccc cgggtggctc ggtggccgaa ccccgaccgc gagcagaagg ccaagggcgc
     8221 gctgtccggc gcccgggccg gccggctccc gtcaacagga ggctctgatg gaggtcgtcc
     8281 tccgcgaggt ccggcgcccc gatctcgaca ccttcttcgc gctgatgcag gacccggaag
     8341 ccgcgcgcat ggcagcgttc acccccgggg acgcgggcga ccgggcccgg ttcgacgccc
     8401 gctgggaacg ggtcctgcgc acggacgacg tcaaccgcac cgtcgtcgcc gacggggtcg
     8461 tggcgggaca cgccgccgtg tacgggccgc caggtgagcg cgaggtgacg tactggatcg
     8521 accgtgccct gtgggggcgc ggtgtggcga cctcggccct gcgggcactg ctgctgctgg
     8581 taccggaacg gccgctgcac gcccgggcgg cggccgacaa cgcgggctcc ctgcgggtac
     8641 tggccaagtg cgggttcacg gtggcggggc ggggcaccgg cttcgcccac gcgcggggcg
     8701 gggagaccga cgagctgctg ctccggctcg accgctgaag acggcgcggg cgcggccctc
     8761 cgtccggcgg ccgagccccg cacgggggag gaccggacgc ggccggccgg gggcggcctc
     8821 ccggaacgga cggcgccggc cgggcggtcc cggacaggcg agtcccgggc aggcgagtcc
     8881 cggggggcga accctcccgg gcaggtcggt cccggtcggc ggctcaccgc cgcagaacgt
     8941 gctcggcccg acccgttcgg ggaatcacgc cggggaatca cgccggggaa ccccgccggg
     9001 gaaccccgct gagggaatca cgccggggaa ccccgcccgg ggacgccctc ccgggaaacc
     9061 ccgcccgggg cgctcccctc ccctgatgag ggttcggtga gaaaaccgac ctgtccggaa
     9121 cggtactccc cgtgcgactc gtccttctcc gagatgaaca agacgatcag gcgcaccgcg
     9181 gtcttcagcc tgctcctggt gttcgccctg ctgctgcggg cgacctgggt gcaggcctac
     9241 gaggccaagg cgctcgcgga cgaccagcac aaccggcgga acaccatcgc gcagtacgcg
     9301 cagccgctcg gcgacatcgt cgtggccggt tccccggtca ccggctcgca gcggacgggg
     9361 ggaggcgatc tcgcgtacaa gcgcacgtac acggacggcg cgctgtacgc acccgtcacc
     9421 ggctacagct cacaggcgta cggggccacc cagctcgaag gcgtctacgg cgatgtgctc
     9481 gacggcacgg acagccggct gaagaacccg ctggacgtcc tcatcgacaa gcaggcccag
     9541 cccggggacg tcctcaccac catcgacccg gcggtccagc aggcgggctt cgaggcgctc
     9601 ggcggcaaga agggcgccgc ggtcgccgtc gaccctgcca ccgggcggat cctggccgtg
     9661 gtgagcactc cgtcgtacga cccgtcgcgg atcagcggga ccacggacgg cgaggcgtgg
     9721 cagcagctca ccggggatga ggaccggccc atggtcaacc gggccctgcg gcagccgctg
     9781 ccgcccggct cgaccttcaa actggtggtc gccgcggcgg cgctggagga cgggctgtac
     9841 tcgtccgtgg acgagccgac cgacagcccc cagccctaca ccctgccgaa cacccggacg
     9901 gtgctggtca acgagaacgc ttccgccccc tgcgaggacg ccacgatccg caccgccctg
     9961 cggtactcct gcaacaacgt cttcggcagg atggcggagc agctcggcaa ggacaagctc
    10021 cgggcgatgg cggagaagtt cggcttcaac gactccgagc aggacgtccc cgtgcgcgcc
    10081 gccacgagtg tgtacccgac ggacatggac gaggcgcaga cggcactgac cggcatcggg
    10141 cagttcgagg tcacggccac cccgctgcag atggccatgg tgtccgccgc cctggccaac
    10201 gacgggctgc tggcgtcgcc gcacatggtg tccagggtcg tggacgcgga cgggaccaca
    10261 ctgcgggagt acccggactc cgacaccgag cggatcgtgt cctcgcggac ggcggagcag
    10321 ctgcgcagcg cgatggtcac ggtcgtggag gacggcaccg gcagcaacgc ccggatcgac
    10381 ggcgccgagg tcggcggcaa gacgggcacc gcccagcacg gcgaggaca
//
