LOCUS       NZ_KZ819178            11368 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. CS131 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ819178
VERSION     NZ_KZ819178.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS131
  ORGANISM  Streptomyces sp. CS131
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7968990)
  AUTHORS   Malmierca,M., Ceniceros,A., Mendez,C., Salas,J.A. and Olano,C.
  TITLE     Genome analysis of twelve Streptomyces spp. associated with Peruvian
            leafcutter ants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7968990)
  AUTHORS   Malmierca,M., Ceniceros,A., Mendez,C., Salas,J.A. and Olano,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.88x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003116755.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 09:38:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,994
            CDSs (total)                      :: 6,912
            Genes (coding)                    :: 6,769
            CDSs (with protein)               :: 6,769
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 41 of 143
            Pseudo Genes (incomplete)         :: 122 of 143
            Pseudo Genes (internal stop)      :: 12 of 143
            Pseudo Genes (multiple problems)  :: 31 of 143
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:40:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7505167
            Orig. end                         :: 7516535
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ819178.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Professor JA Salas
            laboratory.
FEATURES             Location/Qualifiers
     region          1..11368
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="36"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11368
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11368
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6368](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6368
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            193..1260
                     /locus_tag="DBP20_RS33520"
                     /old_locus_tag="DBP20_33540"
     CDS             193..1260
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955794.1"
                     /locus_tag="DBP20_RS33520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_33540"
                     /product="iron ABC transporter permease"
                     /protein_id="WP_109181128.1"
                     /transl_table=11
                     /translation="MTFVDTAPGPARRARRSLRAPGVRLGGLVLLVAVVACAGLFAGRG
                     VTPEGVRAVLLGAGDDPTAEHIVLRLRLPRVLVALVAGACLGVAGLVLQSALRNPLAGP
                     EVTGVTPGAVLGAVTATGLGLAGWESPTAVVVAACIGGFAGAGLLWMLAGRDRGDPEQT
                     AVYGVLVSAVLAGLTAVVLLVAPGELGSVVQWLIGSTEGRVWQHWNLLWPWAACWGAAA
                     WLLAGPLTLLRCGDEQAAAAGLSTGRGRGAALVCAVALTAGAVSAVGALGFVGLLVPHL
                     ALAVFGADLRVTLPGAALLGAAVVCGADAAAQLLSRLLATVLDADRLTLPVGALTTCVG
                     AGLLLVVARRRADER"
     gene            1299..2144
                     /locus_tag="DBP20_RS33525"
                     /old_locus_tag="DBP20_33545"
     CDS             1299..2144
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059071.1"
                     /locus_tag="DBP20_RS33525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_33545"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_109181695.1"
                     /transl_table=11
                     /translation="MNSGVPKVPDVVRAEGLAFGYPGRPVLRGVDLSIRAGELVALIGL
                     NGCGKSTFLKLAAGLLTPSAGRVLLDGDDVARLPRRTAARRVALLHQSAPSVPGLTVRQ
                     LVRQGRYAARGPLGMLREGDDEVTSRALADVGVTAWADRPVDALSGGERQRVRLAMALA
                     QDAPVLFLDEPTTYLDLRHQLEVLQTVVRLRAERRLTVVMVLHDLNHAARFADRIVALR
                     EGRIAADGPPAEVVTRHLLAEVLGVDGRVGTDDVGGWPVCYPDHPLDPLQLLRNENRIH
                     "
     misc_feature    2171..2187
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 19.44"
                     /tool="antismash"
     gene            2213..2347
                     /gene="amiA"
                     /locus_tag="DBP20_RS36460"
     CDS             2213..2347
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508296.1"
                     /locus_tag="DBP20_RS36460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="streptamidine family RiPP"
                     /protein_id="WP_244330664.1"
                     /transl_table=11
                     /translation="MEQNKVFTPIADQGQLAHLSATHSNALVENPFDDAVEADTAAEK"
     misc_feature    complement(2220..2234)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.28"
                     /tool="antismash"
     assembly_gap    2392..2445
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2507..3451
                     /locus_tag="DBP20_RS33530"
                     /old_locus_tag="DBP20_33550"
     CDS             2507..3451
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059073.1"
                     /locus_tag="DBP20_RS33530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_33550"
                     /product="hypothetical protein"
                     /protein_id="WP_109181129.1"
                     /transl_table=11
                     /translation="MTRSRLAPGAGIVTVPGSAPVLRTSGGEFLRIDTGGVTGEALVRR
                     LVGEEAPDEHPQSAELARLVEAFETGGHAVSAPPRAPLAGRTVHVLGDPLLAGPVARCA
                     GAEGAEVQPITPDKVAELVRATPDHRTEGRLAVVWCLDSPVAEGLWDAADRLPDRRIAW
                     LRCHREGSHVWTEPLAAAAGDVTSRHVRLRRLAATPAHRELAAYWAGHRTPDTGPHPTA
                     ASAALIAALLTADLIAWAEAEAEAEAEAEAEGVGGVGRQSAGLPARRRLRRIDLRDLTF
                     AEHPVLPVPDVAPLPAPAPAGTRSRSDTAGARP"
     gene            3448..5004
                     /locus_tag="DBP20_RS33535"
                     /old_locus_tag="DBP20_33555"
     CDS             3448..5004
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377868.1"
                     /locus_tag="DBP20_RS33535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_33555"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_109181130.1"
                     /transl_table=11
                     /translation="MNTRTVSVLAPDGAPLATDVCLPDGDRPRPAVLIRTPYGRDAHRA
                     ELRGWAAHGFAALAQDVRGRHGSPGEWHPYRDHEKADGAATVAWVRAQAWSNGEVVAVG
                     ASYAAYCALVTALDAPGAAPGPVHRDGVPDAVIAAVPALGLTETAREPGGPERLWARAG
                     WWAAHGDRSDSDPDALERALADDPRLLEHLPVARLAERLATGLGRELPSWPRLWADRQR
                     GRLIALGSAARLPLLAVGGTRDPFADDTVALWRDWGGPARLLLGPWGHRLTADRSAPAR
                     ARVNLGALYVRWVRAALAGRLEPTRHGAIALGDSGRWHSTHRAVSPPEAEHRWPFGSPT
                     GLRLLHGAEFSADPERPVRSDDLAVPVGDGHADRCLLLSPPLPRPLDLAGAAVAKINAT
                     ADTPSADWAVRLTALDPSGRADPLAFGIVRRTGPPGEAADITVPLGTLGRRLPAGTRLR
                     AEIAGHHFPAHARNPHTGDDPVTATRLAPSRRTVNPRGSALHLPVVARRRYVEPVPEIC
                     R"
     gene            5001..6368
                     /locus_tag="DBP20_RS33540"
                     /old_locus_tag="DBP20_33560"
     CDS             5001..6368
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059075.1"
                     /locus_tag="DBP20_RS33540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_33560"
                     /product="YcaO-like family protein"
                     /protein_id="WP_109181696.1"
                     /sec_met_domain="YcaO (E-value: 4.2e-45, bitscore: 153.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSALPLEALVDPVSGIVRAVAPVEHPAGAPPRYTAMTADVADAR
                     RLGAWPADRVSLGTTFGDPGGAWIAAVAEAVERYCGNRIPPSGHPQEPRRATAAELTAE
                     GARLYGPGALPAYAPWQYGRPGFPYAELTPDTPALWTRAVENGEPCWAPVALTHLNWRQ
                     GELRSLPRTHHLNYAGIATGQGLDDAVERGLLEVVERDALELWWHLDGPTRGIDSASVP
                     GLTEDLAGCGLDVHIVEMPSEFAPCVAALVHDPGRGMYAAGFACRYDPAEAARKAVLEA
                     VHTWVFTQGTVDADGWVYRAIEAGMLARGLYLDHRADRRYLDDCGPEFAAVRDLGAHVQ
                     VWLDVRMAPLARRFTEPALGVVPVTAIAPGSRTALDAALAAGGHRVITADLTTEDVAET
                     TLRVARVLVSGLIPNAPAAFGYFGCPRFAEAALARGWRTQRPAGPQDFTLAPPPHM"
     gene            6368..7855
                     /locus_tag="DBP20_RS33545"
                     /old_locus_tag="DBP20_33565"
     CDS             6368..7855
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129282.1"
                     /locus_tag="DBP20_RS33545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_33565"
                     /product="nitroreductase"
                     /protein_id="WP_109181131.1"
                     /transl_table=11
                     /translation="MERASMPDTPDRPAGPARGAVAVPPAAPDLLRAALADARSPRVPG
                     LAPYAPERPFPWNAPVTANAGELGDAIDLDRVLRLSLAAPVGTPSRLRPVPSAGALHPV
                     RAHLLLGQGCSLPPGRYAYDPRTHRAHLRGPAPDGVPPGALVVLAVVASRTVAHYGHRA
                     WPLLLLDTGHAAAALALAGATTTDVLVSLDADGSLLSAAAGLPDAPDWQDAWPGTEPEL
                     PLAAILLTPPGGPSGIEPPLRAWAALPRGSAPTPRPGADTTPPRELAAARHLLHHIGEA
                     PGRPGGTWHPASRPGQVTDEALESRRSAPPEDLHRPPGKELLSRILATAENVRPDGPAW
                     TVAVGNAAPALYTAAPGGQGLDVRAAGDARPTLAHWAAGQQWIATSGAVLLAHGCPDDA
                     SHATVRSSHLVAGYAAGAAQAHATALGLRSRPIGSWQQADLGAAFGDTPGRDWIVHGLA
                     LGAPPSAADSLTTEATPATPPTASAAPAAPSGEEERP"
     gene            7852..9591
                     /locus_tag="DBP20_RS33550"
                     /old_locus_tag="DBP20_33570"
     CDS             7852..9591
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955801.1"
                     /locus_tag="DBP20_RS33550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_33570"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_109181132.1"
                     /transl_table=11
                     /translation="MMLHPELLRAARTARRPLGLATALLAAVTATHLAQAVLLALVLSR
                     IAQGETGALAPLLAAVIAVVLVRAALGRAQRLTAVTAGATVRVRLRDTLMERLGALGPT
                     SITGARAGAVRATLVDGVEGVDAYLSRYLPQLLITAAVPPLLLVAVALVEPYAALALAP
                     ALLLALFGPRAWDRLLARRGKEHWDTYEALAADYLEALQGMPTLRNAGAVGRVRERLER
                     RSTALHRSTVAKLRVSLVDTGLTDLAVQGGTVAAVLVACSSAATGRTAATGTYLLLLLA
                     SECFRPVRDLSREWHAGYLGVSAADGIAALRTAEAAVPDRGTVTAAWESAPEICFQDVH
                     FTHPGGARPALDGVSFTAPAGRTTAVVGPSGAGKSTVLSLLLRQRDPDGGRVSVSGTDT
                     AAYTLASLRRGIAVVSQETYLFHATIAENLRIARPSATDGQLRAAARTAGIDEEIARLP
                     GGYATLVGERGATLSGGQRQRLALARALLADAPVLILDEATSAVDERGQDRIVRALATA
                     GRGRTCVVVAHRLDAVRHADHIVVLDAGRVAATGDHTTLLAGGGVYASLVAAGHTAEEE
                     RAA"
     misc_feature    11339..11353
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 17.84"
                     /tool="antismash"
ORIGIN
        1 ccagccggcc cggctcgcgc tcgccccggt cgagacgccc gtcggcgcct ggaccgccct
       61 ggtcggcgtg cccgtcgggg tcctgctgct gaaacggggg cggcggcccg cccgggaccg
      121 ggggcccgtg tccctgcccg cgccggcgac tccgtcggac gcccccgctt ccgtactgcg
      181 gaaggcggaa cgatgacctt cgtcgacacc gcgcccggac cggcccgccg cgcccgccgg
      241 agcctgcgcg cgccgggcgt ccggttgggc ggcctggtgc tgctcgtcgc cgtcgtcgcc
      301 tgcgccggac tcttcgccgg ccggggcgtc acccccgaag gcgtgcgggc cgtgctgctc
      361 ggggccggcg acgaccccac cgccgagcac atcgtgctcc gactccggtt gccccgcgtg
      421 ctggtggccc tggtcgccgg ggcctgcctc ggagtggccg gactcgtgct ccagtcggcc
      481 ctgcgcaacc cgctggccgg acccgaggtc accggcgtga cacccggtgc ggtactcggt
      541 gcggtgaccg cgaccggcct cggcctcgcg ggctgggagt cccccacggc cgtggtcgtc
      601 gcggcgtgca tcggcggctt cgccggagcg ggactgctgt ggatgctggc cgggcgggac
      661 cggggtgacc cggagcagac cgccgtctac ggggtcctcg tctcggcggt gctcgccgga
      721 ctcaccgcgg tcgtcctcct ggtggccccc ggtgaactcg gcagtgtcgt ccagtggttg
      781 atcggctcca ccgagggccg ggtctggcag cactggaatc tgctctggcc gtgggccgcc
      841 tgctggggcg cggcggcctg gctgctcgcc gggccgctga ccctgctgcg ctgcggcgac
      901 gaacaggccg ccgccgctgg cctgtccacc ggccggggta ggggagcggc gctcgtctgc
      961 gcggtggcgc tgaccgccgg cgccgtgtcg gcggtgggcg ccctcggatt cgtcgggctg
     1021 ctggttcccc atctggccct ggccgtcttc ggcgccgatc tgcgcgtcac gcttccgggc
     1081 gccgcactgc tcggagcggc ggtggtgtgc ggggcggacg ccgcggccca actgctgtcc
     1141 cggctgctgg ccaccgtgct cgacgcggac cggctgaccc ttccggtcgg cgccctgacc
     1201 acctgtgtgg gagcggggct gctgctggta gtggcacggc gacgggcgga cgagagatga
     1261 ggaggagaac agtggagacg agcgcgacgg agagcgcgat gaacagcggt gtgccgaaag
     1321 tcccggacgt cgtccgggcg gaggggctgg ccttcggcta ccccggacgc ccggtgctcc
     1381 gcggggtgga cctgagcatc cgggccggtg aactggtcgc cctcatcggg ctgaacggct
     1441 gcggcaagag caccttcctc aagctcgccg ccggactgct caccccgagc gccgggcgcg
     1501 tcctgctcga cggcgacgat gtggcgcggc ttccccgccg cacggccgcc cggcgcgtgg
     1561 cactgctcca ccagtcggcg ccctccgtcc cgggactgac cgtgcgtcaa ctcgtacgcc
     1621 aggggcgcta tgccgcccgg gggccgctcg ggatgctgcg ggagggcgac gacgaggtga
     1681 cgtcgcgggc gctcgcggac gtcggtgtca ccgcctgggc cgaccggccc gtggacgcgc
     1741 tgtccggcgg cgaacgccag cgtgtccgcc tggccatggc gctggctcag gacgcgcccg
     1801 tgctgttcct cgacgagccg accacctatc tcgatctgcg gcaccagctg gaggtgctcc
     1861 agacggtcgt acggctgcgc gcggagcgtc ggctgacggt ggtgatggtg ctgcacgacc
     1921 tcaaccacgc cgcccggttc gccgatcgga tcgtcgcgct ccgggaaggc cgcatcgccg
     1981 ccgacgggcc cccggccgag gtggtcaccc ggcatctgct ggccgaagtc ctcggcgtgg
     2041 acggccgggt ggggaccgac gacgtgggcg gctggcccgt ctgttaccca gatcaccctc
     2101 tcgacccgct ccagctattg cgtaatgaaa atcgtattca ttaatgtctg tgctgcggca
     2161 cgcgccaacg ccgcgaacca cacccaaccg atgtgaatgg aggcttcaac tcatggagca
     2221 gaacaaggtg ttcaccccga tcgccgacca gggtcagctc gctcacctct ccgccaccca
     2281 ctcgaacgcc ctcgtcgaga accccttcga cgacgccgtc gaggccgaca ccgcggccga
     2341 gaagtaagcg actcctgccg ctgaaggcag gtctcccgcg tggcgcgggg gnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgggc caagcgtgcc
     2461 gtccccgcct tccccgcctt ccttcccttc cgacccggag gattccgtga cccgcagtcg
     2521 actcgctccc ggtgccggga tcgtcaccgt tcccggctcc gcccccgtgc tccggacctc
     2581 cggaggggag ttcctgcgca tcgacaccgg cggcgtcacc ggggaagccc tggtgcgccg
     2641 gctcgtgggc gaagaggcac cggacgaaca cccgcagtcc gccgaactcg cccggctcgt
     2701 cgaggcgttc gagaccggtg ggcacgctgt gtcagccccg ccccgggctc cgctcgccgg
     2761 ccgcaccgtc catgtcctcg gcgaccccct gctcgccggg cctgtggcgc gctgtgccgg
     2821 ggcggaaggg gccgaggtcc agccgatcac cccggacaag gtggcggagc tcgtccgggc
     2881 caccccggac caccgcaccg aaggccgtct cgccgtggtg tggtgcctgg actcgcccgt
     2941 ggccgaaggg ctctgggacg cggcggaccg gttgccggac cgacgtatcg cctggctgcg
     3001 ctgccaccgt gagggctccc acgtctggac cgagccgctt gccgcggccg ccggagatgt
     3061 cacctcccgg catgtccgac tccgcagact cgcggccacc cccgcccatc gcgaacttgc
     3121 cgcgtactgg gccggacacc gcacccccga caccggaccg caccccaccg cagcgtccgc
     3181 agcgctgatc gcggcgctgc tcaccgccga cctgatcgcc tgggccgaag ccgaagccga
     3241 agccgaagcc gaagccgaag ccgaaggagt gggcggagtg ggtcggcagt cggccggtct
     3301 cccggcccgc cgtcggctgc gccggatcga tctgcgcgat ctcaccttcg ccgaacaccc
     3361 cgtgctcccc gttccggacg tcgcgccgct gcccgcgccg gcaccggcgg ggacgcgttc
     3421 acggtccgac accgccgggg cccgcccgtg aacacccgta ccgtgtccgt cctcgcgccg
     3481 gacggcgccc cgctcgccac cgacgtatgc ctcccggacg gggaccgccc ccgccccgcc
     3541 gtcctgatcc gcaccccgta cgggcgcgac gcccaccgcg ccgagctgcg cggctgggcg
     3601 gcgcacggat tcgccgccct cgcccaggac gtacgcggcc gccacggctc accgggggag
     3661 tggcacccgt accgggacca cgagaaggcg gacggcgcgg ccaccgtcgc ctgggtgcgt
     3721 gcacaggcgt ggagcaacgg agaggtcgtc gccgtcggcg cctcctacgc cgcgtactgc
     3781 gccctcgtca ccgcactcga cgcccccggc gctgctcccg gtcccgtaca ccgcgacgga
     3841 gtccccgacg ccgtcatcgc cgccgttccc gcgctcgggc tcaccgagac cgcgcgcgag
     3901 cccggggggc ccgagcggtt gtgggcccgg gccggctggt gggcggccca cggcgaccgg
     3961 agcgactccg acccggacgc cctggagcgc gcgctggccg acgacccccg gctgctcgaa
     4021 caccttccgg tcgcccgcct ggcggagcgg ctggccacgg gtctcgggcg tgaactgccg
     4081 tcctggccgc ggctctgggc cgaccggcaa cgcggccgcc tcatcgcgct cgggtccgcc
     4141 gcccgcctgc cgctgctcgc ggtcggcggc acccgcgacc ccttcgccga cgacaccgtg
     4201 gccctctggc gcgactgggg cggaccggct cgtctgctgc tcggcccctg gggacaccgc
     4261 ctcaccgccg accggtccgc acctgcccga gcgcgtgtca acctcggtgc cctgtacgta
     4321 cggtgggtcc gcgccgcgct cgccgggcgc ctggagccga cccgccacgg ggccatcgcc
     4381 ctcggcgaca gcggccgttg gcacagcacc caccgggcgg tttcccctcc cgaggccgag
     4441 catcgctggc ccttcggttc ccccaccggt ctacggctgc tccatggagc cgagttcagc
     4501 gcggacccgg agcgccccgt gcgctccgac gacctcgccg taccggtcgg ggacggccac
     4561 gccgaccgct gtctgctgct ctcgccgcct ctgccccgcc ctctcgatct ggcgggggcc
     4621 gccgtcgcca aaatcaacgc caccgccgac acgccgtcgg ccgactgggc cgtacgtctg
     4681 accgcgctcg acccgtccgg ccgggccgac ccgctcgcct tcggcatcgt ccggcgcacc
     4741 ggacctcccg gcgaagccgc cgacatcacc gtgccgctcg gcaccctagg gcgcaggctg
     4801 cccgccggga ccaggctgcg ggccgagatc gccggacacc acttccccgc ccacgcccgc
     4861 aacccgcaca ccggcgacga cccggtcacg gccacccggc tcgccccgtc ccgccgcacc
     4921 gtgaaccccc ggggcagcgc cctccacctg cccgtcgtcg cccgacgccg ctacgtcgag
     4981 cccgtaccgg agatatgccg ttgaccagcg ccctgcccct cgaagccctc gtcgaccccg
     5041 tcagcgggat cgtccgcgcc gtcgcccccg tcgaacaccc cgccggtgca ccgccccgct
     5101 acaccgcgat gaccgccgac gtcgccgacg cccgccgcct cggcgcctgg cccgccgacc
     5161 gggtctcgct cggcaccacg ttcggcgacc ccgggggcgc ctggatcgcc gcggtggccg
     5221 aggccgtgga gcgctactgc ggcaaccgca tcccgccgtc cggccacccc caggagcccc
     5281 ggcgggccac cgcggccgag ctgaccgccg agggcgcccg cctctacggc cccggcgcgc
     5341 tgccggccta cgcaccgtgg cagtacggcc gcccgggctt cccgtacgcc gaactcaccc
     5401 ccgacacacc ggcgctgtgg acccgggccg tcgagaacgg ggaaccctgc tgggcccccg
     5461 tcgccctgac ccacctcaac tggcggcagg gagagctgcg ttcactgccg cgcacccacc
     5521 acctcaacta cgcgggcatc gccaccgggc agggcctcga cgacgccgtc gagcgcgggc
     5581 tcctcgaagt cgtcgagcgc gacgcgctgg agctgtggtg gcatctggac ggacccaccc
     5641 gcggcatcga ctcggcgagc gtacccgggc tcaccgagga cctggccggc tgcgggctgg
     5701 acgtgcacat cgtggagatg ccctccgagt tcgccccctg tgtcgcggcc ctcgtgcacg
     5761 accccggacg cggtatgtac gcggcggggt tcgcctgccg ttacgacccg gcggaagcgg
     5821 cccgcaaggc cgtcctggag gccgtgcaca cctgggtgtt cacccaaggc acggtcgatg
     5881 ccgacgggtg ggtgtaccgg gcgatcgagg ccgggatgct cgcccgcgga ctctacctcg
     5941 accaccgggc cgaccggcgc tatctcgacg actgcgggcc cgagttcgcc gctgtccgcg
     6001 acctcggcgc ccatgtccag gtctggctgg acgtccggat ggccccgctc gcccgccgct
     6061 tcaccgaacc cgccctgggc gtcgtcccgg tcaccgcgat cgcaccgggc agccgcaccg
     6121 cgctggatgc ggccctcgcc gccgggggcc accgcgtcat caccgcggac ctcacgacgg
     6181 aggacgtcgc cgagaccact ctgcgcgtcg cccgggtgct cgtctccggg ctgatcccta
     6241 acgccccggc agccttcggc tacttcggct gcccgcgctt cgcggaagcg gcgctcgccc
     6301 ggggctggcg cacccaacgg cccgcagggc cgcaagactt caccctcgcc ccacccccgc
     6361 acatgtgatg gagcgcgcca gcatgcccga cacgccggac cgtcccgccg ggcccgctcg
     6421 tggggcggtt gccgtcccgc ccgccgctcc ggacctcctg cgcgctgcac tcgcggacgc
     6481 ccggtcgcca cgcgttccgg ggctcgcccc gtacgcgccg gagcgaccgt ttccctggaa
     6541 cgcgcccgtg accgcgaacg ccggcgaact cggggacgcg atcgacctgg accgtgtgct
     6601 gcggctctcc ctggccgcgc ccgtcggcac accgagccgg ctgcgacctg ttccctcggc
     6661 gggcgccctg cacccggtgc gtgcccacct cctgctcggg cagggctgtt cgctgccgcc
     6721 cgggcgctat gcctacgacc cgcgcaccca ccgggcccac ctgcggggcc cggcccccga
     6781 cggcgtgccc ccgggcgccc tcgtcgtcct tgccgtcgtc gcgtcccgga ccgtcgcgca
     6841 ctacggtcac cgggcctggc cgctgctgct gctcgacacg ggacatgccg ccgccgccct
     6901 cgcgctggcc ggggccacga ccaccgatgt gctggtctcc ctcgacgcgg acggttccct
     6961 cctgtccgcc gccgccggac tgcccgacgc cccggactgg caggacgcct ggccgggcac
     7021 cgagccggaa ctgcccctcg cggcaatcct gttgacgccc ccgggcggcc catccggcat
     7081 cgagccgccg ctgagagcct gggccgcgct gccccgcggc tcggccccga cgccacgacc
     7141 aggagccgac accacaccac cccgtgaact ggccgcggca cggcacctgt tgcaccacat
     7201 cggcgaagcg cccgggcggc ccggcggcac ctggcacccg gcgtcgcgcc ccgggcaggt
     7261 gaccgacgag gccctggagt cgcgccgcag cgcccctccc gaggatctgc accgcccgcc
     7321 cggcaaggag cttctgtccc gcatcctggc caccgccgag aacgtgcggc cggacgggcc
     7381 cgcgtggacc gtggccgtgg gcaatgcggc cccagccctg tacaccgcgg cacccggcgg
     7441 gcagggcctc gacgtccgcg ccgcgggcga cgcgcgcccc accctcgccc actgggccgc
     7501 gggccaacag tggatcgcga cctcgggcgc ggttctcctg gcccacggct gccccgacga
     7561 cgcctcgcac gccaccgtcc gcagcagcca cctcgtcgcc ggatacgcgg ccggcgcggc
     7621 ccaggcgcac gccaccgcac tgggcctgcg ctcacgcccc atcggctcct ggcaacaagc
     7681 ggacctgggc gcggcgttcg gcgacacccc cggacgcgac tggatcgtgc acgggctggc
     7741 cctcggcgca cccccatcgg cagcggactc cctcacgacc gaagccactc cagccacccc
     7801 acccacggca tccgccgccc cggcggcccc gtccggggag gaagaacgcc catgatgctc
     7861 caccccgaac tcctgcgcgc cgcgcgcacc gcgcggcgcc cgctcggcct cgccaccgca
     7921 ctcctcgcgg cggtcaccgc aacccatctc gcccaggccg tcctcctcgc cctcgtgctc
     7981 tcccggatcg cgcaggggga gacgggcgcc ctcgcccccc tgctcgcggc cgtgatcgcc
     8041 gtcgtcctgg tccgagccgc cctcggccgc gcccagcgcc tgaccgccgt cacggccggc
     8101 gccaccgtac gggtgaggct gcgcgacacc ctgatggagc ggctcggcgc gctcggcccc
     8161 acctcgatca ccggtgcgcg ggccggagcc gtacgggcca cgctggtgga cggggtcgag
     8221 ggcgtcgacg cgtacctctc gcgctatctg ccccaactcc tcatcaccgc cgccgtaccg
     8281 ccgctgctgc tcgtcgccgt cgctctcgtc gagccgtacg cggccctcgc cctcgcgccc
     8341 gccctgctgc tcgccctctt cgggccgcgc gcgtgggacc ggctgctggc gcgacgcggc
     8401 aaggaacact gggacaccta cgaagccctc gccgccgact acctggaagc cctccagggg
     8461 atgccgacgc tgcgcaacgc cggtgccgtg ggccgcgtac gggaacggct ggagcggcgg
     8521 tcgaccgccc tgcaccggag caccgtcgcc aaactgcggg tctcgctcgt cgacaccggc
     8581 ctcaccgacc tggccgtcca gggcggcacg gtcgccgccg tcctcgtggc gtgctcctcg
     8641 gccgccaccg gccgcaccgc cgccaccggc acctacctcc tgctgctgct cgcctccgaa
     8701 tgcttccgcc ccgtccgcga cctctcccgc gaatggcacg cgggctacct cggggtgtcg
     8761 gcggccgacg gcatcgccgc cctgcggacc gccgaggccg ccgtcccgga ccggggcacc
     8821 gtgaccgccg cctgggagag cgcgcccgaa atctgtttcc aggacgtgca cttcacccac
     8881 ccgggtggtg cccggcccgc cctcgacggc gtgtccttca cggcccccgc cgggcgcacg
     8941 accgccgtcg tcggcccgtc cggcgcgggc aagtcgaccg tgctgagcct tctgctgcgg
     9001 cagcgggacc cggacggggg ccgcgtctcc gtgtccggca cggacaccgc cgcgtacacc
     9061 ctcgcctcgc tgcgccgggg catcgccgtc gtctcgcagg agacctatct cttccacgcc
     9121 acgatcgccg agaacctccg catcgcccgc ccgtccgcca cggacggaca actgcgcgcc
     9181 gccgcccgca ccgccgggat cgacgaggag atcgcccggc tgccgggcgg ttacgccacg
     9241 ctcgtcggtg aacgaggcgc cacgctctcc ggcggacagc gccaacggct cgccctggcc
     9301 cgcgcgttgc tcgccgacgc tccggtcctg atcctcgacg aggccaccag cgccgtcgac
     9361 gaacgcggtc aggaccggat cgtccgggca ctggcgacgg ccggccgcgg ccgcacctgc
     9421 gtcgtcgtcg cccaccggct cgacgccgtc cgccacgccg accacatcgt cgtcctcgac
     9481 gcgggccgcg tggccgcgac gggcgaccac accaccctgc tcgcgggcgg cggcgtctac
     9541 gcctcgctcg tcgcggccgg acacaccgca gaggaagagc gcgccgcatg accccgaccc
     9601 aggctccgga cccggcccag gcacagttcc ttgccccgcc cacgaacagg tcccctgact
     9661 cggccccgac cggcgccctg cgctccctcc tcccggtcct cggcgcgcac cgcgccaccg
     9721 tgctccgcac ctgcctggcc gccctcgtcg accaggccgc cctggtggcc ctggtcaccc
     9781 tcgccgcgca caccgtgggc accgccgtca tcgagcaccg cccgcccgcc cccgggaccg
     9841 tcgccgtcct gatcgggctc gtcctcctcc gggcgctggc cacctggcgc gagatggacc
     9901 tctcgcacga cctcgcctac cgggtcctgg ccgaactgcg cgtgagggtc ttcgacggac
     9961 tggcccgcag cgcccccgcc cggatcgcgg gccggcgcag cggcgacctc gcggccaccg
    10021 cgctcgggga cgtcgaggca ctggagttct tctacgccca cgccatcgcc caactcctcg
    10081 cctccggcgt ggtcttcacg gtgtcggcgg ccctgctggc agccctcggg ccctggctgc
    10141 tgctcgccgt cgtacccgcc gccctcctcc tggtctggtc gccgctgatc gaggcgcgcg
    10201 gacgggccga acgcgggcac cgcacccgga gcgcgctcgc cgagctgtcg tcggagacgg
    10261 tggagagcgt cgacggactg cgcgagctcc tgatgacggg gtcgctgaac cgcaggcggg
    10321 cccggctccg gtccgccggg cggcgcctcg cccgcgccca gcgcgccgaa cagtcgtggg
    10381 agaccggtgc gggcgcggcc cgcgacctgc tggtcgtcgc cgccgtcatc ggggtcgtgg
    10441 cggccgccgc cgacgccgcg tccgccggac gcctcgacgg ggcatgggcc ccggccgcca
    10501 tggccctcgc cctgggcgcc ctggccccgg tcgcggaatc ggcggccgcg ctcggcaggg
    10561 cgggtggtct gcgcgccgcc gcggcccggg tcagggccgc cgtgcacgcc cccgcgggag
    10621 cgcccgccgc caccgacccc cgccccgtac caccgggccc gctcggcctg cggctgcgcg
    10681 gcgtactgtt cggctacggc ggagcggccg tactggacgg cgtcgacctc accgtccggc
    10741 cgggggagac cgtggccctc gtcggagcgt ccggggccgg caagtcgacc tgcgcccacc
    10801 tcctggcccg ctactgggac ccggccgaag gcgccgtgga actcgtgccc cccgccccgg
    10861 cccaccccgt cgacctgcgg gacctcgccg aggacgacct gcgcgccgcc gtgtccgtcg
    10921 tggggcagga ggccccgctc ttccacggca cgctcgccga gaacctccgc ctcggcgccc
    10981 ccgacgcgac ggcggacgag ctggacgcgg tggcccggat ctgcggcgtc gacaccgtcg
    11041 ccgacgccct gcccgacggt cttgacaccg ccgtcggcga gcgcggcgcg actctctccg
    11101 gcggtcagcg cgcccgggtg gccctggccc ggggtctgct gagcggcccg cgcgtcctcg
    11161 tcctggacga gacgaccgcc cacctggacc accggggcga cgcggaactg gcccgggcac
    11221 tcgcggccga ctctgccacc cgggcgaccc tggtgatcgc ccaccgcccg gccaccgtcc
    11281 gcagggccga ccggatcgcc gtcctggacg gcgggcgcat cgtcgaggag ggcacctggg
    11341 acgagctgat ccgggccgac ggcccact
//
