LOCUS       NZ_KZ819178            10798 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. CS131 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ819178
VERSION     NZ_KZ819178.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS131
  ORGANISM  Streptomyces sp. CS131
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7968990)
  AUTHORS   Malmierca,M., Ceniceros,A., Mendez,C., Salas,J.A. and Olano,C.
  TITLE     Genome analysis of twelve Streptomyces spp. associated with Peruvian
            leafcutter ants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7968990)
  AUTHORS   Malmierca,M., Ceniceros,A., Mendez,C., Salas,J.A. and Olano,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.88x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003116755.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 09:38:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,994
            CDSs (total)                      :: 6,912
            Genes (coding)                    :: 6,769
            CDSs (with protein)               :: 6,769
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 41 of 143
            Pseudo Genes (incomplete)         :: 122 of 143
            Pseudo Genes (internal stop)      :: 12 of 143
            Pseudo Genes (multiple problems)  :: 31 of 143
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:40:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7264795
            Orig. end                         :: 7275593
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ819178.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Professor JA Salas
            laboratory.
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="34"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5798)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1400..1828
                     /gene="panD"
                     /locus_tag="DBP20_RS32505"
                     /old_locus_tag="DBP20_32525"
     CDS             1400..1828
                     /EC_number="4.1.1.11"
                     /GO_function="GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="panD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964661.1"
                     /locus_tag="DBP20_RS32505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_32525"
                     /product="aspartate 1-decarboxylase"
                     /protein_id="WP_093806913.1"
                     /transl_table=11
                     /translation="MLRTLFKSKIHRATVTQADLHYVGSVTIDAALMEAADLLPGELVH
                     IVDIDNGARLETYVIEGERGSGVIGINGAAAHLVHPGDLVILISYAQVDDAEARALVPR
                     VVHVDADNRIVELGTDASAPVPGSGTERSPQAVVAGGR"
     gene            1867..2220
                     /locus_tag="DBP20_RS32510"
                     /old_locus_tag="DBP20_32530"
     CDS             1867..2220
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955645.1"
                     /locus_tag="DBP20_RS32510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_32530"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_109180980.1"
                     /transl_table=11
                     /translation="MAENAAVEIRDDREHGNLVAYEGGESAGTIAYFVMDPAPGALVAV
                     HTVVRPEHEGKGIAGALVREFYAMAAREGVPVVPLCPYAAKWAKRHPDEAPEPSAELVR
                     EAERQLGHHPELF"
     gene            complement(2236..3006)
                     /locus_tag="DBP20_RS32515"
                     /old_locus_tag="DBP20_32535"
     CDS             complement(2236..3006)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072866.1"
                     /locus_tag="DBP20_RS32515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_32535"
                     /product="PIG-L family deacetylase"
                     /protein_id="WP_109180981.1"
                     /sec_met_domain="PIG-L (E-value: 1.6e-22, bitscore: 79.2,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLPSVLAVHAHPDDEALFCGGVLAQHAASGARTAVVTATWAEGT
                     HRAAELARALDVLGAGPPRLLGYADARVPESAPGRARFLDAPLDEAVAALVGHIRDVRP
                     DIVITTDAYGGMTGHPDHVHAHRVTALAVRSAGLPGFCPGAGSPWQPSALHLATHPRSV
                     AVALGRRLARSGTPTEALYCSEDAWITTTVDVRPWLPQKLAAILAHRSEVERGAAPGRI
                     AALPPAVQREVLGAEWYIREDLGRRGGSATELSA"
     misc_feature    3111..3123
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
     gene            complement(3578..4942)
                     /locus_tag="DBP20_RS32525"
                     /old_locus_tag="DBP20_32545"
     CDS             complement(3578..4942)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072867.1"
                     /locus_tag="DBP20_RS32525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_32545"
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_109180982.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 3.6e-38, bitscore:
                     130.0, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 9.3e-08,
                     bitscore: 30.9, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQTTSAPHHGHGTAPPAARFDAVVEAAVQQGLLDEGDPVAGFID
                     TEGVRASVGALHDAFAATPGVLHTFAAKACSLIPVLRLLAGSGMGCEVASPGELRLALD
                     AGFPASRIVLDSPAKTREEIRLALALGVAVNADSLAELRRIDALRNPSTTSVLGLRINP
                     QVGGGSIGPMSTATDTSKFGVALRDPGAREAIVDAFRERPWLTRLHAHVGSQGCPLELI
                     AAGVAQTYELAEEINGTLGVRQITSLDIGGGLPVNFADETVRPTYADYVAALTAAAPGL
                     FSGHYALVTEFGRSLLAKNGFIGARVEYTKDAGGRRIALTHAGAQTATRTALMPDAWPL
                     RIGAFGPDGVPKGGPVLAQDIAGPCCFAGDVVAYGRELPELAEDDVVVLYDTGAYYFST
                     PWAYNSLPRPAVYGFRVARPTGAVTFATVREPQSLDSIAAESGLAHVDALIERDG"
     gene            complement(5001..5798)
                     /locus_tag="DBP20_RS32530"
                     /old_locus_tag="DBP20_32550"
     CDS             complement(5001..5798)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955648.1"
                     /locus_tag="DBP20_RS32530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_32550"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_032774535.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1.5e-87, bitscore:
                     291.6, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVTPSAWDEIEEEARRTFRRHVAGRRVVDVSDPDGP
                     TLAAVGDGHLRDIDPPTPDVLARARTSMPVVEWRVPFTVSRQAVDDVERGSADSDWQPV
                     KDAARTCAFAEDMAIIDGYGAAGITGLRDGSSHEPLPLPADARDYPVAVSQAVTRLRLA
                     GVDGPYRLLLGADAFTEAAETSDHGYPVKTHLSRLVDDEILWAPAVKGGVLLSTRGGDF
                     ELCLGQDLSIGYADHDATSIHLYFHQAFTFRMLTPEAVVGLIA"
     gene            complement(5798..6898)
                     /locus_tag="DBP20_RS32535"
                     /old_locus_tag="DBP20_32555"
     CDS             complement(5798..6898)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456253.1"
                     /locus_tag="DBP20_RS32535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_32555"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_109180983.1"
                     /transl_table=11
                     /translation="MSDAVPDPVAVQPVVAPLTNAALILVGTIEPGGESAVRDVLPDLA
                     AVARSLGFRFPDADLACVAGFGSSAWDRLFTGPRPAHLHSFPELRGPLHHAPATPGDVL
                     LHIRAERMDVCYAWAAQLLDKLGGALRIVDETHGFRYLDHRDLLGFVDGTENPVGDDAR
                     AAALVGEDDDPDFEGGSYVVVQKYLHDLTSWNALTVEEQERVIGRTKLDDVEFPDEDKP
                     ADSHLALSTITDPDGTERDILRANMPFGSFGKGEFGTYFIGYAADPDVTERMLRNMFLG
                     DPPGTHDRILDFSTAVTGSLFFAPRADFLDAPPPLPSLARSGNRPEPVAAPVSPEPAPA
                     PVRQEPVPAASDHGSLRIGSLQESAQ"
     misc_feature    6883..6897
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.0"
                     /tool="antismash"
     gene            complement(7116..8558)
                     /gene="gndA"
                     /locus_tag="DBP20_RS32540"
                     /old_locus_tag="DBP20_32560"
     CDS             complement(7116..8558)
                     /EC_number="1.1.1.44"
                     /GO_function="GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_process="GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="gndA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612845.1"
                     /locus_tag="DBP20_RS32540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_32560"
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="WP_109180984.1"
                     /transl_table=11
                     /translation="MSGTARIGVTGLAVMGRNLARNFARNGFTVALHNRTASRTDALVE
                     QFGDEGTFVAAHTPEEFVAALERPRRLVIMVKAGEPTDAVIQEFAPLLEEGDVIIDGGN
                     AHFEDTRRRERELRERGIHFVGVGISGGEEGALNGPSIMPGGSAESYASLGPMLERIAA
                     QAKDGTPCTAHIGPDGAGHFVKMVHNGIEYADMQLIAEAYDLLRSVAGYSPAQIADVFR
                     TWNTGRLDSYLIEITAEVLAHTDAATGKPFVDIVQDRAEQKGTGRWTVQIALDLGVPVS
                     GIAEAVFARSLSGHADLREAARGLPGPTPEPLDEAGAAAFADKVEQALYASKIVSYTQG
                     FHQIRAGSEAYDWDIDLGSVAAIWRAGCIIRAAFLDRIRAAYDSRPDLVSLLSDKEFGQ
                     EIGAAQEDWRAVVAEAVRQGVPTPGFAAALAYYDGLRAERLPAALTQGQRDFFGAHTYR
                     RTDREGSFHTLWGGDRSEVGAG"
     gene            complement(8710..9252)
                     /locus_tag="DBP20_RS32545"
                     /old_locus_tag="DBP20_32565"
     CDS             complement(8710..9252)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964650.1"
                     /locus_tag="DBP20_RS32545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_32565"
                     /product="DUF5709 domain-containing protein"
                     /protein_id="WP_109180985.1"
                     /transl_table=11
                     /translation="MSDSDWGDDVYQPQEPEASDPAEQLGAEDTLMDPSGTGDPLDEGY
                     APPDRPWVVEGLGTTANERHTGESLETRLTREVPEPDGSLGDGVGDLVDGDGEPWDDEV
                     GVARAGRLTQQADGTVPATLTASDVGIDGAAASAEEAAMHVIPEPGDDDALLEPSPVPG
                     THGSDTATGGTTAPPSP"
     gene            9500..10483
                     /locus_tag="DBP20_RS32550"
                     /old_locus_tag="DBP20_32570"
     CDS             9500..10483
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129108.1"
                     /locus_tag="DBP20_RS32550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_32570"
                     /product="hypothetical protein"
                     /protein_id="WP_109180986.1"
                     /transl_table=11
                     /translation="MGYGQQSPGGDHHQQGPWPPHTGQPPAPGGAPTGPWNAPTEPAGR
                     SVGASGPWSGHTGPPPSPSGGRRRSSVIAVVAATAVVLAAGITGFLVLGDDGDDGKPDA
                     GPTGADVVPPSASPGEPRGGAGDGTAPLVPGWKTVINPKRGIAFDVPVDWAVKSSGWVT
                     YVAENGDPDETPLVGFAAPAILKEKWCRSDDDGNGTQEDTPLASVGSRAEPDARDTTEA
                     ARENARLWVYGSYAQPAKEKVTTGAAKPFTTKSGLTGKVATATSSGVDGTGRCAHDGKA
                     TAFAFENPAGETLSWTFVGVRGVDDEVPEPTVRKILGTVRLVDSTP"
ORIGIN
        1 cccgctcgta gtcaccgagt gcagcgaggg agcgggctcg ggctgtgttg acgtacgcgg
       61 tatcgagcca ggaaccccag acgccgtcgg agtccgcacg ggcgagaagg gcgagcgcgg
      121 tgtcgtaggc cttatgggct tcactggcct ctccgagcac tgcatgtgcg tgagcctcgt
      181 gcagaaccgc gatgacctcc aggcggctcc cgggccgggc ggtgcgtcgg gcagcggtag
      241 cgagcccgag ggcgtcggcg ggatgtccgg tgtcaacggc gagttgggcc tttggctgtt
      301 gcggtagggc tccagcggct ccagaccgac cgaccgcatg tccgcctcgg cccgctcggc
      361 cccgccggcg cgctcctgaa agatcgcccg gcggctctca ttcgcgcacc cgacccgtcc
      421 agcgctgaga tacgccggtc gcggggagaa gatgtttccg cacggcaggt gccgggaccg
      481 ccacggcttc ccgctcccgg ggtagggctc cagcggctcg tatccggccg cccgcatcgc
      541 gatggtgtgc ctggccccgg ctgcttcacc gcgcgagcgc cctgcctcct cgcggtcgga
      601 ggaaggagcg cgggagtatg agcgaaccat gcgacgattc tcgctcttca gggcgagcgg
      661 cgagccctgg ttcgtggttc tcccccagac tcggctcgcg tgggtgccgg ctgtcgtctg
      721 gtttcatgac gaccacccgg ggagagaccc tgctgttccg tcttatagcg tcgcggccgg
      781 ccgcgtgctg atgggttctg tcggatcatt gaatagacgt tcgcgtccca ggcgctcagc
      841 cctgctcgct tacagcaatg tcggcagccg ggcccatagg cgcttccgtt ctcagcgccg
      901 aagggactgt gcgtcgccgg ccggggtcgg cttcctaccg cgcctacggc tcgcgggcca
      961 gccgggcggg aagccgtcgc tcgtgagttc cacgctgccg aggggcctcg gacgggcgac
     1021 agatagcgag ccgacttccg gagcggtcga gggtcgaccg gctgatggcc aaagaggctg
     1081 tgttccgtga accggcgcga tgtcggtgcc cgacagtttg ctggtgcgtg ggcgtccgtc
     1141 gtccgtgtgc agtggggttt ccccgcatgc ctgatgactc atcaggtaag tcagcattcg
     1201 gtcagtatga gtccccacgt gctttggggt gatcgccccc aacagacgcc tgagtgaagg
     1261 gaaagaccca ggtaggaacc cgtctcgacc gacccgagcg atgttcctca caaacgtcgt
     1321 ccggcgcgtg ccggtgtaca gtcgagaccg cccttgacct gcagaaagca ggcagggagc
     1381 cgtctcagag gagtcccaag tgttgcgcac tctcttcaag tccaagatcc accgggccac
     1441 cgtcacccag gccgacctgc actacgtcgg atccgtgacg atcgacgccg ctctgatgga
     1501 ggccgccgac ctgctgcccg gcgagctggt ccatatcgtt gacatcgaca acggcgcccg
     1561 cctggagacg tacgtcatcg agggcgagcg cggctccggc gtcatcggga tcaacggcgc
     1621 cgccgctcac ctcgtgcacc ccggtgacct ggtcatcctg atcagttacg cacaggtcga
     1681 cgatgcggag gcccgtgctc tcgtgccgcg ggtcgtgcac gtggacgcgg acaaccggat
     1741 cgtggagctc ggcaccgacg cctcggcgcc ggtccccggg agcggtacgg agcggagccc
     1801 gcaggccgtc gtcgcgggcg gccgatagcg gcgtccgggt ccaggaccag gagagggcgg
     1861 aagccgatgg cggagaacgc tgctgtggag atcagggacg accgggagca cggcaacctc
     1921 gtggcgtacg agggcggtga gtcggccggg accatcgcgt acttcgtcat ggacccggct
     1981 cccggcgcgc tcgtggccgt ccacaccgtc gtccggcccg agcacgaggg caagggcatc
     2041 gcgggcgcgc tcgtgcggga gttctacgcg atggccgccc gtgagggcgt gcccgtcgtc
     2101 ccgctgtgcc cgtacgcggc gaagtgggcg aagcgtcacc ccgacgaggc acccgagccg
     2161 tcggccgagc tcgtccggga ggcggagcgg cagctcgggc atcaccccga gctgttctga
     2221 ccgtcccggg gcggatcacg cgctcagttc cgtggccgat ccgccccggc gccccaggtc
     2281 ctcccggatg taccactcgg cgcccagcac ctcgcgctga acggccggcg gcagagcggc
     2341 gatccggccg ggtgccgccc ctcgttccac ctcgctgcgg tgcgcgagaa tcgcggccag
     2401 cttctgcggc aaccaggggc ggacgtcgac ggtcgtcgtg atccaggcgt cctccgagca
     2461 gtacagcgcc tcggtggggg tgcccgacct ggccagccgt cggcccagcg cgacggccac
     2521 cgagcggggg tgtgtggcca ggtggagggc gctcggctgc caggggctgc ccgcgcccgg
     2581 acagaacccg ggtagtccgg cggaccgcac cgccagcgcg gtgacccggt gggcgtggac
     2641 gtggtccggg tggccggtca tgcccccgta ggcgtccgtc gtgatcacga tatccgggcg
     2701 tacgtcccgg atgtggccga ccagggccgc cacggcttcg tccagcggcg cgtccaggaa
     2761 ccgggcccgg cccggggccg actccggtac gcgggcgtcc gcgtagccga gcagccgcgg
     2821 tgggcccgcg cccaggacgt ccagtgcccg ggcgagctcg gcggccctgt gcgtgccctc
     2881 tgcccaggtc gccgtgacca ccgccgtacg ggccccggac gccgcgtgct gggccaggac
     2941 gcccccgcag aacagggcct cgtcgtccgg atgggcgtgg acggcgagaa cactcggcag
     3001 gggcatggcg gaacctttca cggcggtggc gacggtcccg gatccgggcg ggcccgggtg
     3061 tcgtgcccgg agcctgccca ctccggaccg ccccgcacct gggcccgtac gtgccgtggt
     3121 caccgggcgc atgtgtggac tcggcaggcg cgggcagccg caccgggaga cggtggagga
     3181 aagctccatc tgtccttttc gtgccgttgt ggaggatcgc gatgacccac ccgtaccccg
     3241 accccgtacc gccctcgccg acccctccgc ccgtgccggg accgcctacg ccggtgccca
     3301 gcccgcctcc cgtgccgccg gggcccggtc cgatgccgcc cggtccggca ccggacccga
     3361 tcccgcagcc gccggcgccc ggccccgacc ccgtaccgga tccggaaccg gagcctcagc
     3421 ccgccggaac ctgaggatcc gggaccggag cctcagccgg ccgagacctg agcatccgga
     3481 accggagcgt caggcccccc cccgggggac ctgaccgggg gagcccccgg gcggcgatga
     3541 gcgccgtccg ggggctcctc ggcctcggac ggcggcctca gccgtcccgc tcgatcaggg
     3601 cgtccacgtg cgccagcccg ctctccgccg cgatcgagtc cagcgactgc ggctcccgta
     3661 ccgtcgcgaa cgtcaccgcc cccgtgggcc gcgccacccg gaatccgtac accgccgggc
     3721 ggggcaggct gttgtatgcc cacggggtgg agaagtagta cgcccccgtg tcgtacagca
     3781 cgaccacgtc gtcctcggcc agctccggca gctccctccc gtacgccacc acgtcccccg
     3841 cgaagcagca cggccccgcg atgtcctgcg ccagcaccgg gccgcccttg gggacgccgt
     3901 ccgggccgaa cgcaccgatc cgcagcggcc aggcgtccgg catcagggcg gtacgggtcg
     3961 cggtctgcgc ccccgcgtgg gtgagggcga tccggcggcc tccggcgtcc ttcgtgtact
     4021 ccacccgggc cccgatgaac ccgttcttcg ccagcagcga ccggccgaac tccgtgacca
     4081 gcgcgtagtg cccggagaag aggccggggg cggccgccgt cagggccgcc acgtagtccg
     4141 cgtaggtggg gcgcacggtc tcgtccgcga agttgaccgg gagcccgccg ccgatgtcca
     4201 ggctcgtgat ctgccggacg cccagtgtgc cgttgatctc ctcggccagc tcgtacgttt
     4261 gggccacccc ggccgcgatc agctccaacg ggcagccctg ggaacccaca tgggcgtgca
     4321 gccgggtcag ccacggccgt tcccggaagg catccacgat cgcctcgcgg gcccccgggt
     4381 cgcgcagggc caccccgaac ttcgacgtgt ccgtcgccgt gctcatcggc ccgatcgagc
     4441 caccgcccac ctgagggttg atccgcagcc cgagcacgga ggtcgtgctc gggttccgca
     4501 acgcgtcgat gcggcgcagt tcggcgaggc tgtcggcgtt gacggccacg ccgagggcga
     4561 gcgccagccg gatctcctcg cgggtcttgg cgggggagtc caggacgatg cgggacgcgg
     4621 ggaacccggc gtccagggcg aggcgcagtt caccggggct cgccacctcg caccccatgc
     4681 cggagccggc cagcagcctc agcaccggga tgagggagca ggccttggcc gcgaaggtgt
     4741 gcagcacccc gggcgtggcg gcgaaggcgt cgtgcagcgc cccgacggag gcgcgtaccc
     4801 cctccgtgtc gatgaacccg gcgaccggat cgccctcgtc cagcagccct tgctggacgg
     4861 ccgcttcgac gacggcgtcg aagcgggcgg cgggcggtgc agtgccgtgc ccgtggtgtg
     4921 gtgcggaagt cgtctgcgtc atacggccag agtccctgat ggctgccccc aggtcttcgg
     4981 ctcggcccga ccggaagcgg tcaggcgatc aggccgacga cggcctccgg cgtcagcatc
     5041 cggaaggtga acgcctgatg gaagtagaga tggatgctgg tggcgtcgtg gtccgcgtaa
     5101 ccgatcgaca ggtcctgccc caggcagagt tcgaagtcgc cgccccgcgt cgacagcagc
     5161 accccgccct tgaccgcggg cgcccacagg atctcgtcgt ccaccagccg gctcagatgc
     5221 gtcttgaccg gatacccgtg gtccgaggtc tcggccgcct cggtgaaagc gtccgcgccg
     5281 agcagcagcc ggtacggtcc gtcgacaccg gccagccgca ggcgcgtcac tgcctggctg
     5341 accgccaccg gatagtcccg cgcgtcggcg ggcagcggca gcggttcgtg cgaggagccg
     5401 tcccgcagcc cggtgatccc ggccgccccg tacccgtcga tgatcgccat gtcctcggcg
     5461 aaggcacagg tgcgtgccgc gtccttgacc ggctgccagt cgctgtcggc ggagccgcgc
     5521 tccacgtcgt ccacggcctg gcggctcacc gtgaacggaa cccgccactc gacgaccggc
     5581 atcgacgtgc gggcccgggc cagcacgtcc ggagtgggcg gatcgatgtc gcgcagatgg
     5641 ccgtcgccca cggcggccag cgtcggaccg tccgggtcgg acacgtcgac gacccggcgg
     5701 ccggccacat ggcggcggaa ggtgcgccgg gcctcctcct cgatctcgtc ccaggcggac
     5761 ggggtgaccg gtgcgagttc gcggtgcaga ttgttcatca ctgggcactt tcctgcaggc
     5821 tgccgatgcg cagcgaaccg tggtcggatg cggccgggac cggctcctgc cggacggggg
     5881 caggcgccgg ttccgggctg accggtgcgg cgaccggttc cggccggttc ccggagcggg
     5941 ccagggaggg caggggcggc ggggcgtcca ggaagtcggc gcgcggagcg aagaagaggg
     6001 agccggtgac cgccgtggag aagtcgagga tgcggtcgtg cgtcccgggc ggatcgccga
     6061 ggaacatgtt gcggagcatg cgctcggtga cgtcgggatc ggcggcgtac ccgatgaagt
     6121 acgtaccgaa ctcgcccttc ccgaagctcc cgaacggcat gttcgcccgc aggatgtcgc
     6181 gttcggtgcc gtccgggtcg gtgatggtgc tgagggcgag gtgggagtcg gcgggcttgt
     6241 cctcgtcggg gaactccacg tcgtcgagct tggtgcggcc gatgacgcgt tcctgctcct
     6301 cgacggtgag agcgttccag gacgtcaggt cgtgcagata cttctgtacg acgacgtagc
     6361 tgccgccctc gaagtccggg tcgtcgtcct cgccgaccag cgccgccgca cgggcgtcgt
     6421 cgccgaccgg gttctccgta ccgtccacga aaccgagcag gtcccggtgg tcgaggtagc
     6481 ggaagccgtg cgtctcgtcc acgatccgca gggctccgcc gagcttgtcg agcagctggg
     6541 ccgcccacgc gtagcagacg tccatccgct cggcccggat gtgcagaagg acgtcacccg
     6601 gcgtcgcggg cgcgtggtgg agcggccccc gcaactccgg gaacgagtgc aggtgtgcgg
     6661 ggcggggccc ggtgaacagg cggtcccagg cgctggagcc gaaccccgcc acacaggcga
     6721 gatcggcatc cgggaagcgg aagccgagcg accgggccac ggccgcgaga tccggcagca
     6781 catcccgtac cgcgctctcg ccgcccggct cgatcgtgcc gaccaggatc agcgccgcgt
     6841 tcgtcaacgg cgccacgacg ggctgtacgg cgacgggatc ggggacggcg tcggacatgc
     6901 gcgcgctcca ggacgaaggt gacaggagaa caggactcgc agccactaag ccacgtcccg
     6961 cagccccccg cacccgcagt ggtccggccg ggtcagcccg catggccccg cccccgcccg
     7021 cacaccggga gcgccctgcc gggtcagccc gactcgggcc gcacaccggt cgtgtcgggg
     7081 cagcccgcct cgaccctcgg accggtcgtt ccgggtcagc ccgccccgac ctcggaccgg
     7141 tcgccgcccc agagcgtgtg gaacgaaccc tcccggtccg tacgccggta cgtgtgtgcc
     7201 ccgaagaagt cccgctggcc ctgcgtgagc gccgcgggca gccgctccgc gcgcagcccg
     7261 tcgtagtacg cgagcgcggc ggcgaagccc ggcgtcggca ctccctggcg cacggcctcg
     7321 gccaccaccg cccgccagtc ctcctgcgcc gcgccgatct cctgcccgaa ctccttgtcg
     7381 gagagcagac tgaccaggtc gggccggctg tcgtaagcgg cgcggatacg gtcgaggaac
     7441 gccgcccgga tgatgcaccc cgcccgccag atcgccgcca cggacccgag gtcgatgtcc
     7501 cagtcgtagg cttcgctgcc cgccctgatc tggtggaagc cctgcgtgta cgagacgatc
     7561 ttggaggcgt acagggcctg ctccaccttg tccgcgaagg ccgccgctcc ggcctcgtcg
     7621 agcggctcgg gcgtgggccc cggcaggccg cgcgccgcct cccgcagatc ggcgtggccc
     7681 gagagcgacc gggcgaagac cgcctcggcg atccccgaca ccgggacccc gagatcgagc
     7741 gcgatctgta cggtccagcg cccggtgccc ttctgctcgg cccggtcctg cacgatgtcg
     7801 acgaacggct tcccggtcgc cgcgtccgtg tgggcgagga cctccgccgt gatctcgatc
     7861 aggtacgagt cgagccggcc ggtgttccac gtacggaaga cgtcggcgat ctgtgcgggg
     7921 gagtacccgg cgacggagcg cagcaggtcg tacgcctcgg cgatcagctg catgtccgcg
     7981 tactcgatgc cgttgtggac catcttcacg aagtgacccg cgccgtccgg gccgatgtgc
     8041 gcggtgcacg gagtgccgtc cttcgcctgc gcggcgatcc tctccagcat cgggcccagc
     8101 gacgcgtagg actccgccga accgcccggc atgatgctgg gcccgttcag cgcgccctcc
     8161 tcgccgccgg agatccccac cccgacgaaa tggatcccgc gctcgcgcaa ctccctctcg
     8221 cggcggcggg tgtcctcgaa gtgggcgttc ccgccgtcga tgatgacgtc gccctcctcc
     8281 agcagcggcg cgaactcctg gatcaccgcg tcggtgggtt cccccgcctt caccatgatc
     8341 accagacgcc gggggcgttc cagcgcggcg acgaactcct cgggcgtgtg cgcggcgacg
     8401 aacgtgccct cgtcgccgaa ctgctcgacc agggcgtcgg tgcgggacgc ggtgcggttg
     8461 tgcagggcga cggtgaagcc gttgcgggcg aagttccggg cgaggttgcg gcccatgacc
     8521 gcgagtccgg tgacgccgat ccgagcagtg ccgctcatgt gtgtgctcct ggggatgcga
     8581 gtgcgtgggg tgcggtgggc gccgctcttc gggggctgag gtgcggccgc aggagtacgg
     8641 gtccggggac agccgtgttc aggtgtgggc gctcacggcg gcccgctcag gggcccaggc
     8701 gcggcccgtt cagggcgagg gcggggcggt cgtgccaccg gtggcggtat cggacccgtg
     8761 agtgcccggg accggcgagg gctccaggag cgcgtcgtcg tcccccggct cggggatcac
     8821 gtgcatcgcg gcctcctcgg ccgacgccgc cgctccgtcg atgcccacgt cgctggcggt
     8881 gagggtggcc gggaccgtcc cgtccgcctg ctgggtcagc cgcccggcac gggcgacgcc
     8941 cacctcgtcg tcccagggct cgccgtcgcc gtccacgaga tcgcccacac cgtcgccgag
     9001 cgagccgtcc ggctcgggaa cctccctcgt gaggcgggtt tcgagggact cgccggtgtg
     9061 ccgctcgttg gccgtggtgc cgagtccctc gaccacccac gggcggtccg ggggcgcgta
     9121 gccctcgtcc agcggatcgc cggtgccgga ggggtccatg agcgtgtcct ccgccccgag
     9181 ctgctcggcg gggtcggaag cctcgggttc ctggggctgg tagacgtcgt ccccccagtc
     9241 gctgtcgctc atggcgcgtc ctttcctccg gggatctccg ggagtaccga ataccgcacg
     9301 taccgaatac cgctgtcccc tgtacgcaat tccactccgg tcccgctccg gccgcaaagg
     9361 gaagatcgga ttcgggtgcc ccgcacaggt ggcgtgaaac ggtccgtacg caggtcgcgc
     9421 ccggttcccc ggcgcacgga gacttatggg acgccgggcc tttcgaatcg tcgaacagct
     9481 cggcataggg tggccctcca tggggtacgg acagcagtcg cccgggggcg atcaccacca
     9541 gcagggacca tggccgccgc acacgggaca gccgccggct cccgggggcg cgccgaccgg
     9601 cccctggaac gcgcccaccg agccggccgg gcggtccgtc ggggcctccg gcccctggag
     9661 cgggcacacc ggcccgcccc cttccccgtc cggcggccgt cgccgcagct cggtcatcgc
     9721 cgtcgtcgcg gccaccgcag tcgtgctggc cgccggaatc accggattcc tggtgctcgg
     9781 ggacgacggg gacgacggga agcccgacgc gggacccacc ggagcggacg tcgtcccgcc
     9841 gtccgcgtcc ccgggggagc cccggggcgg agccggggac ggcacggcgc ccctcgtgcc
     9901 cggctggaag acggtgatca accccaagcg cggcatcgcc ttcgacgtac cggtggactg
     9961 ggcggtgaag tcgtccggat gggtgacgta cgtcgcggag aacggcgacc ccgacgagac
    10021 cccgctcgtc ggattcgccg ccccggcgat cctcaaggag aagtggtgcc ggtccgacga
    10081 cgacggcaac ggcacccagg aggacacccc gctggcctcc gtgggctccc gggccgagcc
    10141 cgacgcccgc gacaccaccg aagcggcccg ggagaacgcc cgcctctggg tctacggcag
    10201 ctacgcccag ccggcgaagg agaaggtcac caccggggcc gcgaagccgt tcaccacgaa
    10261 gtccggcctc accgggaagg tggcgacggc cacctcgagc ggagtcgacg ggacgggccg
    10321 ctgcgcccac gacggcaagg cgaccgcgtt cgcgttcgag aacccggcgg gggagaccct
    10381 ctcctggacg ttcgtcggtg tccgcggcgt cgacgacgag gtgcccgagc cgaccgtgcg
    10441 gaagatcctg ggtacggtcc ggctcgtcga cagcaccccc tgaccgcaac caagatcaac
    10501 tcactggcgg cggcaccggt gacctgcgat gctgacccgg ccgcgccgaa cgccggacgg
    10561 ggcaggagcg ggggaaccgg tggctgagga cgtcttcacg acgaagtccg gcacggtacg
    10621 ggggcggcgc gccgcccggg accacggcat cgtcgtcgta cgcggactcc cgtacgcggc
    10681 cccgccgttc ggtgccctcc ggttccggga gccgcagccg ccggacccct gggacggcgt
    10741 gcgcgactgc gccgcgttcg gcccggtggc cccgcagtcg gccgaactgc cgggcgcg
//
