LOCUS       NZ_KZ819178            11329 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. CS131 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ819178
VERSION     NZ_KZ819178.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS131
  ORGANISM  Streptomyces sp. CS131
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7968990)
  AUTHORS   Malmierca,M., Ceniceros,A., Mendez,C., Salas,J.A. and Olano,C.
  TITLE     Genome analysis of twelve Streptomyces spp. associated with Peruvian
            leafcutter ants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7968990)
  AUTHORS   Malmierca,M., Ceniceros,A., Mendez,C., Salas,J.A. and Olano,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.88x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003116755.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 09:38:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,994
            CDSs (total)                      :: 6,912
            Genes (coding)                    :: 6,769
            CDSs (with protein)               :: 6,769
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 41 of 143
            Pseudo Genes (incomplete)         :: 122 of 143
            Pseudo Genes (internal stop)      :: 12 of 143
            Pseudo Genes (multiple problems)  :: 31 of 143
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:40:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6434020
            Orig. end                         :: 6445349
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ819178.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Professor JA Salas
            laboratory.
FEATURES             Location/Qualifiers
     region          1..11329
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="28"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11329
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11329
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6329](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6329)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    384..399
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.74"
                     /tool="antismash"
     misc_feature    complement(935..951)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.3"
                     /tool="antismash"
     gene            1362..2090
                     /gene="hemQ"
                     /locus_tag="DBP20_RS28995"
                     /old_locus_tag="DBP20_29015"
     CDS             1362..2090
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="DBP20_RS28995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_29015"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_006128414.1"
                     /transl_table=11
                     /translation="MSAPETVTSSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRA
                     GYADEVQELFDQLAAKDITVRGTYDLSGLRADADIMIWWHAETSDQLQDAYNLFRRTKL
                     GRALDPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARLHVRE
                     EVPFYTGRRKSVADLVAGLA"
     gene            complement(2247..3809)
                     /locus_tag="DBP20_RS29000"
                     /old_locus_tag="DBP20_29020"
     CDS             complement(2247..3809)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (101-476). E-value:
                     5.8e-20. Score: 63.9. Matches aSDomain:
                     nrpspksdomains_DBP20_RS29000_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="DBP20_RS29000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_29020"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_109180529.1"
                     /sec_met_domain="PF00561 (E-value: 3.7e-25, bitscore: 87.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAALYTSIGSLVLTALAAAPAGAWDRPGSAEARGTATAAARAT
                     AAGIDFAACPEEEMLPDSLKCGTVKVPLDYADPDGRQLELTVSRTPATGPAKERQGAFV
                     YNPGGPGASSITFPLAGELPEWKEIAEAYDLVGYAPRGVNGSSAPLSCQDPSAYTKGPT
                     DAPTHPTQEYKERRVARAKAYARGCAEHAGETLRHYTSLNNARDLDVLRAALGESRLTF
                     MGASYGTYFGALYATLFPSHVRRMVFDSAVDPDPEQIWYRSNMGQSLAFEARWEDFRRW
                     VAKHDDVYHLGTTPEAVQRRYDEVRAELAVKPAGGKVGPGQLHSAFLGAGYYDDYWAMR
                     ATALSEYVRGNPEPLVAQASPRAVAAKDNENAQAVYTAVECNDAPWPEDFEVWDRDHTE
                     LARVAPFEAWDNAFSNLPCAFWPAPRQRPLDVRVGWGELPPTLILAAERDAATPYKGAL
                     ELRKRLPGSSLVTERDAGTHGIGGAGNTCVDDHLRRYLLTGEVPGRGADCAAHPEPNPV
                     SLD"
     aSDomain        complement(2382..3506)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DBP20_RS29000_Abhydrolase_1.1"
                     /evalue="5.80E-20"
                     /label="DBP20_RS29000_Abhydrolase_1.1"
                     /locus_tag="DBP20_RS29000"
                     /protein_end="476"
                     /protein_start="101"
                     /score="63.9"
                     /tool="antismash"
                     /translation="AFVYNPGGPGASSITFPLAGELPEWKEIAEAYDLVGYAPRGVNGS
                     SAPLSCQDPSAYTKGPTDAPTHPTQEYKERRVARAKAYARGCAEHAGETLRHYTSLNNA
                     RDLDVLRAALGESRLTFMGASYGTYFGALYATLFPSHVRRMVFDSAVDPDPEQIWYRSN
                     MGQSLAFEARWEDFRRWVAKHDDVYHLGTTPEAVQRRYDEVRAELAVKPAGGKVGPGQL
                     HSAFLGAGYYDDYWAMRATALSEYVRGNPEPLVAQASPRAVAAKDNENAQAVYTAVECN
                     DAPWPEDFEVWDRDHTELARVAPFEAWDNAFSNLPCAFWPAPRQRPLDVRVGWGELPPT
                     LILAAERDAATPYKGALELRKRLPGSSLVTERDAG"
     gene            complement(3958..4995)
                     /locus_tag="DBP20_RS29005"
                     /old_locus_tag="DBP20_29025"
     CDS             complement(3958..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072528.1"
                     /locus_tag="DBP20_RS29005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_29025"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_204163128.1"
                     /transl_table=11
                     /translation="MNTLALSATLAVVVSSVLLVARAVAERSRRPSPGPGVALHDLYEV
                     AFLNGGPARVVDTALTALHADGRLAVGGPGIVAVQRSQANDPVERAVFEALAAAPNGAL
                     HVLREAVMRHPAVQEVGDGLAARGLLVAPHRLRPLRRWGLTQGIACLFALPLSLFLTFV
                     QFVTDDSLDFSVPFIMKMLPAIVIGSFTGLIVANSSAGRLTASGRHAAQSFRAAYAHVV
                     APAHLVATLGLRALPDPVFQAQLMAAARLGAPRRRSSGRRLTGSTATGVTAVGVATVWC
                     AASGPGGSSCGGSTGGGSGSSCGGGSGCSSGSGCGGGSGSSCGSSSGSSCGGGGGGSSC
                     GGGGS"
     gene            complement(5001..6329)
                     /locus_tag="DBP20_RS29010"
                     /old_locus_tag="DBP20_29030"
     CDS             complement(5001..6329)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072529.1"
                     /locus_tag="DBP20_RS29010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_29030"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_109180531.1"
                     /sec_met_domain="DUF692 (E-value: 3.6e-91, bitscore: 303.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELGIGIGWRPEIAAEVEALSGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVALGLGGADRPAPDRLAALAARAELLGAPLVTEHIAFVRAGGPLTASP
                     RLEAGHLLPVPRTWDALDVLCENVRIAQDSLPVPLALENIAALITWPDEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNLGQDPAKALDELPVEAIAYVHVAGGVERNGVWHDTHA
                     HPVSGPVLDVLAELRSRVDPPGVLLERDDAFPPGAELAGELDAIRATLRRAAPSAGPGP
                     DRGTAPAPGGTAGAEGPTAAPAPVSAATRDRTAVAQTALLSALVAGTPAPEGFDHRRLR
                     VQSRALAAKRADVVAKVAPELPEILGDGYRAAFLAYAGSRTMSGGYRRDALDFAEHVLI
                     AGGPADPAARRRLAYWWQDRSGSRPPRRTTRLVRAARAVLVGK"
     gene            complement(6451..7197)
                     /locus_tag="DBP20_RS29015"
                     /old_locus_tag="DBP20_29035"
     CDS             complement(6451..7197)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072530.1"
                     /locus_tag="DBP20_RS29015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_29035"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_176741787.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATLGALAFPVWSYADRSGTGEANLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSEATKAAGDHLVVGHTDLDRRAR
                     DVAAKLGVELPNQPTTQQQEWLRELTAASGDEYERKFANILRAAHGKVFGLIGQVRHTT
                     RNTLIRQLASDANQTVLDHITMLEATGLVDFDALAREAASGSTASPSGPSMPRDGQAPL
                     APVPATPTGDQSFTSRPVPPTVMPMP"
     gene            complement(7399..8127)
                     /locus_tag="DBP20_RS29020"
                     /old_locus_tag="DBP20_29040"
     CDS             complement(7399..8127)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072531.1"
                     /locus_tag="DBP20_RS29020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_29040"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_109180532.1"
                     /transl_table=11
                     /translation="MTSHDTPSPASGSPFRSGPTDRDQAPQFVLPLVLHLEKTDPPART
                     DALRAAARAVLTILSDERSLGDGEWAEAMRDWQDARIRKVVRRARGAEWRKAAALDGIT
                     VNGEDGAEVRVFPPIPLDGWPKELAKLQVSGTDLDDPEPPAAPDLSGTVLWLNPDVDMS
                     AGKTMAQAGHGAQLAWWELSETERKAWREAGFPLSVATPGAERWRELTASGLPVVRDAG
                     FTEIAPGSCTVIADHPALRR"
     gene            8199..8873
                     /locus_tag="DBP20_RS29025"
                     /old_locus_tag="DBP20_29045"
     CDS             8199..8873
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="DBP20_RS29025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_29045"
                     /product="AIM24 family protein"
                     /protein_id="WP_032777597.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHMAQPATAPGMTLQNAKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLALMAVRGQGEAWFAHEAANCFIVELDQGDSIT
                     INGRNVLCFDPTLSYEIKTVKGAGMVGGGLFNSVFTGYGKLGLMCEGTPIVIPVTAAQP
                     VYVDTDAVVGWSQQLTTSLNRSQSVGSMVRGGSGEAVQLMLQGEGFVIVRPSEARPEKT
                     SN"
     gene            8876..9781
                     /locus_tag="DBP20_RS29030"
                     /old_locus_tag="DBP20_29050"
     CDS             8876..9781
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523641.1"
                     /locus_tag="DBP20_RS29030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_29050"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_204163212.1"
                     /transl_table=11
                     /translation="MHLRGVGHRHNPAGRWVLRGVDLALPARALVRVEGGNGMGKSTLL
                     RLLAGIEAPAEGRITGRPARTAYVPERFPAALPLTATGYLVHLGRIHGLRTAEAKRRAG
                     EWLTRFGAAEHARTPLAELSKGTSQKVAVAQALLAAPDLLVLDEAWTGLDTAARTELDR
                     AVAERVAADATVVFVDHDPRRLAGAADLTLRTGDGGVHRATATPDTAPRTLIEAEGPPG
                     APLPQDLPGDPLRERGTATAPDLVRLTVPTPYSDALLTALLTARPPWRIRRVAPVDTPE
                     RPGPPEPIRPSEQPEVSETP"
     misc_feature    9008..9010
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9778..10446
                     /locus_tag="DBP20_RS29035"
                     /old_locus_tag="DBP20_29055"
     CDS             9778..10446
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452202.1"
                     /locus_tag="DBP20_RS29035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_29055"
                     /product="ABC transporter"
                     /protein_id="WP_109180533.1"
                     /transl_table=11
                     /translation="MTALLRYQAALLARSQRWLAPLLLYVAVLAVGVQSGQPVLGSLGF
                     AAAALLPVTAWAVHLCVGQDPPAARAVASAAGGPRRVHLAALLTATGCAAVLAGVTTAV
                     VLLVSSPATDNGTQAVPRPTAALAGVLAALCCVLLGAAVGALSTRPVLRRRGWSVAAAV
                     LGSLLALVTTGSPARYAVTALVTGSRTGTFDPPVLASLGAATLAGAAGFVVCRLAARRG
                     "
     gene            10455..10652
                     /locus_tag="DBP20_RS29040"
                     /old_locus_tag="DBP20_29060"
     CDS             10455..10652
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128422.1"
                     /locus_tag="DBP20_RS29040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBP20_29060"
                     /product="hypothetical protein"
                     /protein_id="WP_109180534.1"
                     /transl_table=11
                     /translation="MDVDDTYCEPPERTASARPAGTAEVPPYAECVLCREPTEYPESTK
                     GATLCPVCAWQEAGRTACSG"
ORIGIN
        1 gcgggtcacc ctcctggagg cgacggaccg gctcggcggc aagctcatga ccggcgaggt
       61 cgcgggcgtc caggtcgacc tgggggccga gtcgatgctc gcccggcgac ccgaggcggt
      121 cgcgctggcc gaggcggtgg gcctcggcga ccggctccag ccgcccgcca ccgccaccgc
      181 gtcgctctgg acgcgcgacg cgctgcgccc catgccgaag ggccacgtca tgggcgtccc
      241 cggcgacccg gcggtcctcg gcgaggtgct gtcccccgag ggcctggccc ggatcgccga
      301 ggagcgcgac ctcaccccga cgcccgtcgg cgacgacgtc gcggtcggcg cgtacgtcgc
      361 cgaccggctc ggccgcgagg tcgtggaccg gctggtggag cccctcctcg gcggggtcta
      421 cgcgggcgac gcctaccgga tctcgatgcg cgccgccgta ccccagctct tcgaggccgt
      481 gcaggagggc ggcccgctgc tggacggcgt ccgccgcatc caggagcggg ccgcagcccg
      541 ccagcagacc ggccccgtct tccagggcat cgcgggcggc atcggcaccc tcccggccgc
      601 cgtcggcgac gccgtacggt ccgggggcgg ggagatcctc accgagaccc ccgtcctcgg
      661 cctgacccgt tcggaatcgg gctgggccgt ccgcaccgac acccggacca tcgccgccga
      721 cggcatcgtc ctcgccaccc ccgcctggtc cgcgggcacc ctgctggccg ccgagtcccc
      781 ggcagcctcc gccgagctgt ccggcgtcga gtacgcctcg atggccctgg tcaccctggc
      841 cttccggcgc accgacatcg aggcgtacga agccctgcgc ggccgctccg gctttctcgt
      901 gccgccggtc gacgggcaca cgatcaaggc gtccacgttc tccagcaaca agtggcagtg
      961 ggtcgccgac gccgcccccg acctgttcgt gctccgcacc tccgtcggcc gctacggcga
     1021 ggaggaccac ctccaccgcg aggacgagga actcgtcgcc gtctccctgc gcgacctcgc
     1081 cgccgccacc ggcctcaccg cccggcccgt cgacaccgag gtcacccgct ggatcggcgg
     1141 cctgccgcag tacccggtcg gccatctgac ccgggtcgcc cgcatccgcg acgaggtcgc
     1201 caagctgtcc gccctgcggg tctgcggcgc ggtctacgac ggggtgggca tccccgcctg
     1261 catcgcgagc gcgcaccgcg cggccgacga gatcgcgagc gagatcatcg ccacgccgac
     1321 cctggttcgg ggcacgggga gcgaggcggg acaatagccg tatgagtgct cccgagactg
     1381 tgacatcaag caagagtccg aacgccggca agaaggccaa ggacctcaac gaggtcatcc
     1441 gctacaccct gtggtcggtc ttcaaactcc gcgacgtcct ccccctggac cgcgcgggat
     1501 acgccgacga ggtgcaggag ctgttcgacc agctcgcggc caaggacatc accgtccggg
     1561 gcacctacga cctgtccggg ctgcgggccg acgcggacat catgatctgg tggcacgccg
     1621 agacctcgga ccagctccag gacgcgtaca acctcttcag gcgcaccaag ctcggccgcg
     1681 cgctcgaccc ggtctggtcg aacatggcgc tgcaccgccc cgccgagttc aacaagtcgc
     1741 acatcccggc gttcctcgcc gacgagacgc cccgcgacta catcagcgtc taccccttcg
     1801 tccggtccta cgactggtac ctgctgcccg acgaggaccg ccgccgcatg ctcgcggacc
     1861 acggcaagat ggcccgaggc tacccggacg tccgcgccaa caccgtcgcc tcgttctcgc
     1921 tcggcgacta cgagtggatc ctcgccttcg aggcggacga gctgtaccgc atcgtcgacc
     1981 tgatgcgcca cctgcgcgcc tccgaggcac ggctccacgt gcgcgaggag gtcccgttct
     2041 acacgggccg caggaagagc gtcgcagacc tggtggccgg gctcgcatag ccagcgagtc
     2101 ccgcaccacc ccaacccgga agatcagaga gtcgggcacc gggcaccgat cgtgccgtcc
     2161 gcctctccag cgacaccggg ttcggcttcg ggggccgtcg caccatcacg gtgcggcggc
     2221 ccccgctccg tacgcccgcc ggagggtcag tccagcgaca ccgggttcgg ctccgggtgc
     2281 gcggcgcagt ccgcaccccg ccccggcacc tcaccggtca gcaggtaccg gcgcagatga
     2341 tcgtcgacgc aggtgttccc cgccccgccg atgccgtgcg tccccgcgtc ccgctcggtc
     2401 accagcgacg aaccgggcag ccgcttccgc agctccagcg cccccttgta cggggtcgcc
     2461 gcgtcccgtt cggccgccag gatcagcgtg ggcggcagct cgccccatcc cacgcggacg
     2521 tccagcggcc gctgccgggg agcgggccag aacgcgcacg gcagattcga gaaggcgttg
     2581 tcccaggcct cgaagggagc cacgcgcgcc agctccgtgt ggtcgcggtc ccagacctcg
     2641 aagtcctcgg gccagggcgc gtcgttgcac tcgacggccg tgtacaccgc ctgggcgttc
     2701 tcgttgtcct tcgccgccac cgcgcgcggg gacgcctgcg cgaccagcgg ctccgggttg
     2761 ccccggacgt actccgacag cgccgtcgca cgcatcgccc agtagtcgtc gtagtagccg
     2821 gcccccagga acgccgagtg cagctgcccc ggcccgacct tgccgccggc cggcttcacc
     2881 gccagctcgg cgcgcacctc gtcgtagcgc cgctgcaccg cttccggcgt ggtccccagg
     2941 tggtacacgt cgtcgtgctt ggcgacccag cggcggaagt cctcccagcg cgcttcaaag
     3001 gcaagcgact gacccatgtt ggagcggtac cagatctgct ccgggtccgg gtcgaccgcc
     3061 gagtcgaaga ccatgcgccg tacgtgggag gggaacagcg tcgcgtacag cgccccgaag
     3121 tacgtcccgt acgacgcccc catgaacgtc agccggctct cgccgagcgc ggcccgcagc
     3181 acgtccaggt cgcgggcgtt gttgagcgag gtgtagtggc gcagcgtctc gcccgcgtgc
     3241 tccgcgcagc cccgcgcgta cgccttggca cgggcgacgc gccgctcctt gtactcctgg
     3301 gtcggatgcg tcggcgcgtc ggtcgggccc ttcgtgtacg cggaagggtc ctggcaactc
     3361 agcggggcgg aggagccgtt cactccgcgc ggggcgtagc cgaccaggtc gtacgcctcg
     3421 gcgatctcct tccactccgg cagctccccg gccagcggga aggtgatgct ggaggcgccg
     3481 gggccgcccg ggttgtagac gaacgcgccc tgccgctcct tcgccggacc ggtggcgggc
     3541 gtccggctga cggtcagttc gagctgccgc ccgtccggat ccgcgtagtc gaggggcacc
     3601 ttcaccgtgc cgcacttcag ggagtccggg agcatctcct cctcggggca ggcggcgaag
     3661 tcgatcccgg cggccgtggc ccgggcggcg gcggtcgcgg tgccccgggc ctcggcggaa
     3721 ccggggcggt cccaggcccc cgccggagcg gcggcgaggg cggtcaggac cagggagccg
     3781 atcgatgtgt agagcgcagc agttctcacg cgcggggcct ctctgcggat gcggagaggg
     3841 atggtcgggc ggcggggcgg acatgtccag taccgccccg gaatgtcccc ggaatccacc
     3901 ctgatggacg ctcaagaacc gtacgtacgc gcccgattca ggtcacgcga gaccggttca
     3961 ggagccaccg cccccacagc tggatccgcc gccgccgccc ccacagctgg agccactgga
     4021 gctgccgcaa ctcgacccgc tgccgccgcc gcacccggac cccgaggagc acccggatcc
     4081 gccgccgcag ctcgacccgg agccgccccc ggtcgacccg ccgcaactgg agccgccggg
     4141 accggacgcg gcgcaccaga ccgtcgccac gccgacggcg gtgacacccg tcgccgtgga
     4201 cccggtgagg cgccgcccgg aggaccgccg acgcggcgcg cccagccgtg ccgcggccat
     4261 cagctgggcc tggaacaccg ggtccggcag ggcgcgcagg cccagggtgg cgacgaggtg
     4321 ggcgggggcc accacgtggg cgtaggcggc ccggaacgac tgggcggcgt ggcgccccga
     4381 cgccgtgagg cggcccgcgg aggagttggc gacgatcagc ccggtgaacg agccgatcac
     4441 gatcgcgggc agcatcttca tgatgaacgg gaccgagaag tccagggagt cgtccgtgac
     4501 gaactggacg aacgtcagga acaggctcag cggcagggcg aacaggcacg cgatcccctg
     4561 ggtcaggccc caccgccgca gcgggcggag ccggtggggc gccaccagca gcccgcgcgc
     4621 cgcgagcccg tcgccgacct cctggaccgc cgggtgccgc atcaccgctt cccgcaggac
     4681 gtgcagggcg ccgttcgggg cggcggccag cgcctcgaac accgcgcgct ccaccgggtc
     4741 gttcgcctgc gaccgctgta cggcgacgat gccgggcccg cccacggcca gccggccgtc
     4801 cgcgtgcagg gcggtcaggg cggtgtccac gaccctggcc ggaccgccgt tcaggaacgc
     4861 gacctcgtac aggtcgtgaa gggcgacgcc ggggcctggg gaggggcgtc gggaccgctc
     4921 ggccaccgct ctggcgacaa gcagcaccga cgagacgacg accgccaggg tcgccgacag
     4981 agcgagcgtg ttcacgacgg tcacttcccc acgaggacgg cacgggccgc ccggaccaga
     5041 cgggtggtac ggcgcggggg ccgggagccc gaccggtcct gccaccagta cgccagccgg
     5101 cgccgcgccg ccggatcggc gggaccgccc gcgatgagca catgctccgc gaagtccagg
     5161 gcgtcgcggc ggtagccccc ggacatggtg cggctcccgg cgtacgcgag gaacgcggca
     5221 cggtagccgt cgccgaggat ctccggcagc tcgggggcca ccttggcgac gacgtcggcg
     5281 cgcttggcgg ccagcgcccg gctctgcacc cgcagccgcc ggtggtcgaa gccctcgggg
     5341 gccggggtcc cggcgaccag ggcggagagc agtgcggtct gtgcgacggc ggtacggtca
     5401 cgggtggcgg cggacacggg ggcgggtgcg gccgtagggc cttcggcgcc cgcggtgccg
     5461 ccgggcgcgg gagcggtacc ccggtccggc cccgggcccg ccgagggcgc cgccctccgc
     5521 agcgtcgccc ggatcgcgtc cagttcgccc gccagctccg cgcccggcgg gaaggcgtcg
     5581 tcccgctcca gcagcacgcc cggcggatcg acccgggagc gcagctcggc cagtacgtcc
     5641 agtacgggtc cggacaccgg gtgggcgtgg gtgtcgtgcc agacgccgtt cctctcgacc
     5701 ccgcccgcca catggacgta cgcgatggcc tccaccggca gttcgtccag cgccttcgcc
     5761 gggtcctggc ccaggttgac gtggttggtg tgcagattgg ccacgtcgat cagcagccgc
     5821 accccggtcc gctcgaccag ctccgccagg aactgcccct cggtgagctc ctcgtcgggc
     5881 caggtgatca gcgcggcgat gttctccagg gcgagcggca ccggcagcga gtcctgcgcg
     5941 atccgcacgt tctcgcagag cacgtccagc gcgtcccagg tgcggggcac cggcagcagg
     6001 tgccccgcct ccagcctcgg cgacgcggtc agcggccccc cggcccgcac gaacgcgatg
     6061 tgctcggtca ccaggggcgc ccccagcagc tcggcgcgcg ccgcgagagc ggcgagccgg
     6121 tcgggggcgg gccggtccgc gccgccgagg ccgagcgcca ccccgtgcgg cacgacggtc
     6181 accccgcgct cgcgcagccg taccagcgag tccgggaggt ggtcggcgca gaggttctcc
     6241 gcgaccgcct ccacccagtc gatcccgctc agcgcctcca cctcggccgc gatctccggc
     6301 cgccagccga taccgatgcc cagttccatg gtgcccccgt ccccctgctc acctcgttct
     6361 gttcaggggt catggccccg tcgcgtggcg atgaacccga ccggtgggac gttcagagct
     6421 tcatttgagg ttccggaccc ggcgccgtcg tcacggcatc ggcatcaccg tgggcggcac
     6481 cgggcgtgag gtgaaggact ggtcaccggt cggtgtcgcc ggcaccggag ccagtggggc
     6541 ctggccgtcg cggggcatcg acggaccgga aggactggcc gtcgatccgc tcgccgcttc
     6601 gcgcgccagg gcgtcgaagt cgacgagccc ggtggcctcc agcatggtga tgtggtccag
     6661 cacggtctgg ttggcgtcgc tcgccagctg ccggatcagg gtgttccggg tggtgtggcg
     6721 gacctggccg atcaggccga agaccttgcc gtgggcggcg cgcaggatgt tggcgaactt
     6781 tcgctcgtac tcgtccccgc tcgccgccgt cagctcccgc agccactcct gctgctgggt
     6841 ggtcggctgg ttgggcagct ccaccccgag cttcgccgcg acgtcccggg cccgccggtc
     6901 caggtccgtg tgcccgacga cgagatggtc gccggcggcc ttcgtcgcct cgctgggggc
     6961 ccgttcgatc gcctgctgcc cggcgggcag ctcccacagc ccggcgagcc gcaccttgac
     7021 caggaagtcc cggtcggtgg cggagagcgg cccccactgc gtggcgacac tggaggcgtt
     7081 gaggttcgcc tcgccggtgc ccgaacggtc ggcgtaggac cagacgggga aggcgagcgc
     7141 gccgagtgtg gcgaccagtg ccgcgatgat gagggccgtg ccattgatgc gtcgcaacaa
     7201 ggtgtctccc gggccgaggc gtgacagcgt cgatcgaaca ggcgtcaatc aaccgccaat
     7261 cgactggctg tgcaggctac ttggcggtaa tgccgatagg gcagtaccgg gagatacgta
     7321 ggaggaacga gaagtgttca gcgcgcgggc gatggggtcg cggctccgcg acggggccca
     7381 ctgtggccgc cgtcccgttc agcggcgcag cgccgggtgg tcggcgatca ccgtgcagga
     7441 gcccggggcg atctcggtga accccgcgtc gcgcaccacg ggcagcccgc tcgccgtcag
     7501 ctcccgccag cgctccgccc cgggggtggc cacggagagc gggaagccgg cctcgcgcca
     7561 ggccttgcgc tcggtctcgg acagctccca ccaggcgagc tgagccccat gcccggcctg
     7621 ggccatcgtc ttgcccgccg acatgtcgac atcggggttc agccacagga ccgtgcccga
     7681 gagatcgggc gcggcgggcg gctcggggtc gtccaggtcc gtgcccgaca cctggagctt
     7741 ggccagctcc ttgggccagc cgtccagcgg gatcggcggg aacacccgta cctcggcacc
     7801 gtcctcgccg ttcaccgtga tgccgtcgag tgccgccgcc ttgcgccact ccgcaccgcg
     7861 cgcccggcgc acgaccttgc ggatccgggc gtcctgccag tcccgcatcg cctccgccca
     7921 ctcgccgtcg ccgagggacc gctcgtcgga gaggatcgtc agcaccgccc gggcggcggc
     7981 ccgcagcgcg tcggtgcggg cgggcgggtc cgtcttctcc agatggagca ccaggggcag
     8041 cacgaactgc ggtgcctggt cgcggtcggt cgggccgctg cggaacgggc tcccggaggc
     8101 gggggagggg gtgtcatggc tggtcaccgt cccagtctgc caggcggacg aggtcacctg
     8161 cttggcggaa cgaccgggtc cgggtgagga tgctccgcat gaagagcgat ctcttttcca
     8221 gtgagcacat ggcccagccg gccaccgccc ccggtatgac cctgcagaac gcgaaatcca
     8281 tcaagtacgc cgtcaacggg gagatgcacg cccggcaggg ctcgatgatc gccttccgcg
     8341 gcaacctcca gttcgagcgc aagggccagg gcatcggcgg catgctcaag cgcgccgtca
     8401 ccggcgaggg cctcgcgctg atggccgtcc ggggccaggg cgaggcctgg ttcgcgcacg
     8461 aggcggcgaa ctgtttcatc gtggagctgg accagggcga ctcgatcacc atcaacggcc
     8521 gcaacgtcct gtgcttcgac cccacgctct cctacgagat caagaccgtg aagggcgccg
     8581 gaatggtcgg cggcggcctc ttcaacagcg tcttcacggg ctacggcaag ctcggcctga
     8641 tgtgcgaggg caccccgatc gtgatccccg tgacggccgc ccagccggtg tacgtggaca
     8701 cggacgcggt cgtcggctgg agccagcagc tcaccaccag cctgaaccgc tcccagagcg
     8761 tcggctcgat ggtccgcggc ggctccggcg aggccgtcca gctgatgctc cagggcgagg
     8821 gcttcgtcat cgtccggccc agcgaggccc ggcccgagaa gacctccaac tgagcctgca
     8881 cctgcggggc gtgggccacc gccacaaccc cgccggccgc tgggtgctgc gcggggtcga
     8941 cctcgccctg cccgcacggg ccctcgtccg cgtcgagggc ggcaacggca tgggcaagtc
     9001 gaccctgtta cggctgctcg ccgggatcga ggcccccgcc gaggggcgga tcaccggccg
     9061 cccggcccgc acggcgtacg tccccgagcg cttcccggcc gcgctgcccc tcaccgccac
     9121 cggctacctc gtccacctcg gccggatcca cggactgcgg accgccgagg cgaagcggcg
     9181 ggcgggggag tggctgaccc ggttcggggc ggcggagcac gcccgtaccc cgctcgccga
     9241 actctccaag ggcaccagtc agaaggtcgc cgtcgcccag gcgctgctcg ccgcaccgga
     9301 cctgctcgtc ctggacgagg catggaccgg gctcgacacc gcagcccgca ccgaactcga
     9361 ccgtgccgtg gccgaacgcg tcgcggccga cgccaccgtc gtcttcgtcg accacgaccc
     9421 gcgacgcctc gccggggcgg cggacctcac cctgcggacg ggggacggcg gggtccaccg
     9481 ggcgacagct acgccggaca ccgccccccg caccctgatc gaggccgagg gcccgcccgg
     9541 tgcgccgctg ccccaggacc tcccgggcga cccgctccgc gaacgaggaa cggcgacggc
     9601 ccccgatctc gtacgcctca cggtcccgac gccgtactcc gacgccctgc tgacggccct
     9661 gctgacggcc cgtccgccgt ggcgcatccg gcgggtggcg cctgtcgaca cacctgagcg
     9721 gcctggtccg cccgagccga tacggccgtc cgaacagccc gaggtgtccg agacgccatg
     9781 accgcccttc tccgctatca ggccgccctg ctcgcccgct cccagcgctg gcttgcgccc
     9841 ctcctgctgt acgtggccgt cctcgccgtc ggcgtgcaga gcgggcagcc cgtgctgggc
     9901 tcgctcggtt tcgcggccgc cgccctgctg cccgtcaccg cgtgggccgt ccacctctgc
     9961 gtcggccagg atccgcccgc cgcccgggcg gtcgcctcgg ccgccggcgg accccgacgg
    10021 gtccacctgg ccgcgctgct caccgccacc ggctgcgcgg cggtgctcgc cggagtgacc
    10081 accgcggtgg tcctgctcgt cagcagcccg gccaccgaca acggcaccca ggccgtcccc
    10141 cggccgaccg ccgcgctcgc cggagtgctc gcggccctgt gctgcgtgct gctcggcgcg
    10201 gccgtggggg cgctcagcac ccgcccggtg ctgcgccgcc gtggctggtc ggtagcggcg
    10261 gccgtgctcg gctcgctcct cgcgctcgtg accaccggct ctccggcgcg gtacgcggtc
    10321 accgccctgg tcaccggatc gcggaccggg acgttcgacc cgcccgtgct ggcgtcgctc
    10381 ggcgcggcga cgctcgccgg ggcagcgggg ttcgtcgtct gccggctcgc cgcccggcgc
    10441 ggctgagtag gctcgtggac gtggacgaca cctactgcga gcccccggaa cggacagcat
    10501 ccgcccggcc cgccggaacc gccgaagtcc ctccgtacgc cgaatgcgtg ttgtgccgcg
    10561 agccgaccga gtacccggag tcgacgaagg gcgccacgct ctgcccggtc tgcgcctggc
    10621 aggaggcggg ccggacggcg tgttccggct agggctactg tccggccgat catgtgcctg
    10681 tgatggaaca tgcatgacgt cgcccaggtc ctggcatctg cgacgccatc acggccaggc
    10741 atctggagcg tgagggtctg gcagagtacg tgccaggaag tcggcgtcgg cttctttgac
    10801 ggctgacgtg atttcccggg ccgctcgtct gccgagagcg ccgatggctc gctctatcag
    10861 gaggcggtcg tcccagatgt cgtagccggg caggtgcggc cctggggtga tgctgcgtgg
    10921 gggcccgtgc ctccccagga cggccgtctg cctcagcgag gaacaggggc ggcgcgcggt
    10981 tcaagtcgca gtcctggcgt agagccaggc aaggtcgtgc agccggtatc ttccagactc
    11041 cagggggacg cagatcccgg cgttcgcaag atcctcgagg atcctctccg cttcggcggc
    11101 gctgctgttg agcagggtcc tcacgctctc cacagagacc accggggtgg aacgtgttcc
    11161 aagcgctctg aatacctggg cctgaacgaa ggtgagttgt cgatagctca ggtcgagggt
    11221 ggctctgacc gccaagtctc caacctgcaa ctcgtccaga cgttgccgct cgtcggcgag
    11281 cttgcctgcc aggtcagaga tggaccagtt acggcgggca gcgagtcgg
//
