LOCUS       NZ_BFCH01000024         6792 bp    DNA     linear   CON 22-DEC-2024
DEFINITION  Mycobacterium montefiorense strain BS sequence24, whole genome
            shotgun sequence.
ACCESSION   NZ_BFCH01000024
VERSION     NZ_BFCH01000024.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Mycobacterium montefiorense
  ORGANISM  Mycobacterium montefiorense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium; Mycobacterium simiae complex.
REFERENCE   1
  AUTHORS   Fukano,H., Yoshida,M., Shimizu,A., Iwao,H., Katayama,Y., Omatsu,T.,
            Mizutani,T., Kurata,O., Wada,S. and Hoshino,Y.
  TITLE     Draft Genome Sequence of Mycobacterium montefiorense Isolated from
            Japanese Black Salamander (Hynobius nigrescens)
  JOURNAL   Genome Announc 6 (21), e00448-18 (2018)
   PUBMED   29798927
  REMARK    DOI:10.1128/genomeA.00448-18 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Fukano,H., Yoshida,M. and Hoshino,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2018) Contact:Yoshihiko Hoshino National Institute
            of Infectious Diseases, Mycobacteriology; 4-2-1, Higashimurayama,
            Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Platanus v. 1.1.0
            Genome Coverage                   :: 129x
            Sequencing Technology             :: Illumina Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003112775.1-RS_2024_12_22
            Annotation Date                   :: 12/22/2024 07:39:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,429
            CDSs (total)                      :: 5,376
            Genes (coding)                    :: 5,180
            CDSs (with protein)               :: 5,180
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 91 of 196
            Pseudo Genes (incomplete)         :: 88 of 196
            Pseudo Genes (internal stop)      :: 32 of 196
            Pseudo Genes (multiple problems)  :: 14 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:34:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6792
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BFCH01000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..6792
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>6790](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..>6790)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..4753)
                     /locus_tag="DB708_RS21545"
     CDS             complement(<1..4753)
                     /NRPS_PKS="Domain: Condensation_LCL (5-298). E-value:
                     1.4e-66. Score: 216.5. Matches aSDomain:
                     nrpspksdomains_DB708_RS21545_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (459-846). E-value:
                     1.4e-100. Score: 328.7. Matches aSDomain:
                     nrpspksdomains_DB708_RS21545_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (955-1022). E-value: 4.7e-20. Score:
                     63.6. Matches aSDomain: nrpspksdomains_DB708_RS21545_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1041-1328). E-value:
                     7.8e-87. Score: 283.0. Matches aSDomain:
                     nrpspksdomains_DB708_RS21545_Condensation_LCL.2"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="DB708_RS21545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_165839887.1"
                     /sec_met_domain="AMP-binding (E-value: 5.2e-108, bitscore:
                     348.3, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.1e-18, bitscore:
                     54.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 5.4e-70, bitscore:
                     222.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATIDEYPLAPAQQRLWIIHRLNPLSTAYNAPVVRTLRGNVDPRR
                     LEKALLQLLKRHEQLRATFSLGGAEPTHVIHDVSSFELVYLENVAVKDVSKTITQFIGP
                     FALEHAPLLRAALIRVSDHAKLEPSYVLVMDIHHIVCDLRSEDILVRDLFALYHDLPLP
                     EIGSSYTAQLHRQVEPSAAAGQWRADEEFWLSKFAGELPVLQLLDDHRRPAVFDFRGTI
                     RHFPMGNERTKTLRAFATSHQVPLRDIALTAVFVLLAKYSFQEDLVIGMPDEARCCDDM
                     AEVVGMFMNTLAIRANTHPDKTVRTLMTEVSEDLCESRRHSSYDVRYLVEKLRPPRDPS
                     RNALYDVLFFHQCLQTDTYDGLEVAPRQFIHKKAEVDLTFGLLEIDDQLSFSIEFATSI
                     YRSPTITRMAKHYGAVLDALVSSPDALLADIDILSVDERNRVASFSPPASPYPWKTINA
                     LFEEQVARSPNHPAIRYRNETLDYRELDTRANRVANMLLAHGADTGDVIALWSHPSLDT
                     VAGSLGILKAGCVYLPISPDVPAARIAYMLRASKAKLVVAQDLPGEDLDDEIIELGLAA
                     RYPGDLRPQLERDPAAIACVLFTSGSTGNPKGTLMRHFNVVRTVVDTNHLAIRPSDRML
                     QLATPTFDVSMLEVFGALLNGATLVVADSDSRGDLSRIAALLDGERISVAFMTPALFNA
                     MVDEKLSALGGLRQILTGGDRASADHMRKALARLGRGKLQNGYGPTEATIFTTYYPVDD
                     VAADATNVPIGYPLSNARGAILNGRSPVPVGVVGELCIAGDGLVAGYLDEALTAQKFVI
                     IAGERFYRTGDIAKWHDDGAIEFVGRKDRQVKIRGMRIEPEEIEAVLQSHPWVQEAAVI
                     VRVDAQGDKSLQAFCTRVDDASFTPDELIADAKRHLPGYMVPNSLFVVEALPLDVNGKI
                     DRRRLESTVPEVVKEEAEHPPITEIERTVAAVWREILGLSVVEVHRSFFEYGGHSLTAA
                     ILSSRLEQALEVPVPLREIFEHPTIAALAAQLSGPSRAPHRRRTRVARNPKRAHYPLSF
                     AERMVYAHQHATKRNHGYHNVFPMLIEGPLDIEKLEQALREVVQRHEAFRSAFVLRDGL
                     PVKTVASRADFRLVCAQGTESDVPTVVSRLREAYDLASPPLFRACLLTLGHQRHVLVVA
                     NHHIVSDGVTETLFMHEIGQLYRDEPLDPRPLQYTDYTLWQQREWEEGRYRAQEQHWVE
                     RLRGPLPVLELPLDHPRPSETNFDGHTVTIRLDVEQTSRLRDLAKAHHTTLFTPLFAAY
                     TVLLHKYSGQEDLIVGIPVANRMHADVQSIAGMFVNMVPLRTRPAPGVAFADYLHSVKA
                     EALSAFEHQDYPFERMVHSFQGERDPSRNPLFDTIFAYQSTGPAVLEIEGLRIAPYSLP
                     NASAKVDLTVEVFECSDEIQLSFTYATALFRESTILRMGESFLRLVSDIVLDPGKPLRM
                     LAWLPAGAGFGVYELSVYPRDGSLFGLFEGCVGRFARRVAVRFRDEVLTYAQLHARAES
                     MAVRLLDLGVRPGEPVALMTARSVEMVVGIWAILRVGATYVPIDPGYPQKRVESILRDS
                     GAQVC"
     source          1..6792
                     /collection_date="2010"
                     /db_xref="taxon:154654"
                     /geo_loc_name="Japan:Niigata, Niigata city"
                     /host="Hynobius nigrescens"
                     /mol_type="genomic DNA"
                     /organism="Mycobacterium montefiorense"
                     /strain="BS"
                     /submitter_seqid="sequence24"
     region          1..6792
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..6792
                     /SMILES="NC(C(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(62..103)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0016"
                     /evalue="5.30E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="DB708_RS21545"
                     /protein_end="1564"
                     /protein_start="1550"
                     /score="13.5"
                     /tool="antismash"
                     /translation="WAILRVGATYVPID"
     CDS_motif       complement(611..727)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0015"
                     /evalue="3.30E-17"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="DB708_RS21545"
                     /protein_end="1381"
                     /protein_start="1342"
                     /score="54.8"
                     /tool="antismash"
                     /translation="AEALSAFEHQDYPFERMVHSFQGERDPSRNPLFDTIFAY"
     aSDomain        complement(770..1630)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DB708_RS21545_Condensation_LCL.2
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="7.80E-87"
                     /label="DB708_RS21545_Condensation_LCL.2"
                     /locus_tag="DB708_RS21545"
                     /protein_end="1328"
                     /protein_start="1041"
                     /score="283.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVSDG"
                     /tool="antismash"
                     /translation="AHYPLSFAERMVYAHQHATKRNHGYHNVFPMLIEGPLDIEKLEQA
                     LREVVQRHEAFRSAFVLRDGLPVKTVASRADFRLVCAQGTESDVPTVVSRLREAYDLAS
                     PPLFRACLLTLGHQRHVLVVANHHIVSDGVTETLFMHEIGQLYRDEPLDPRPLQYTDYT
                     LWQQREWEEGRYRAQEQHWVERLRGPLPVLELPLDHPRPSETNFDGHTVTIRLDVEQTS
                     RLRDLAKAHHTTLFTPLFAAYTVLLHKYSGQEDLIVGIPVANRMHADVQSIAGMFVNMV
                     PLRTRP"
     CDS_motif       complement(779..868)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0014"
                     /evalue="3.80E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="DB708_RS21545"
                     /protein_end="1325"
                     /protein_start="1295"
                     /score="41.8"
                     /tool="antismash"
                     /translation="DLIVGIPVANRMHADVQSIAGMFVNMVPLR"
     CDS_motif       complement(1127..1159)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0013"
                     /evalue="1.20E-02"
                     /label="C4_LCL_164-176"
                     /locus_tag="DB708_RS21545"
                     /protein_end="1209"
                     /protein_start="1198"
                     /score="8.6"
                     /tool="antismash"
                     /translation="QYTDYTLWQQR"
     CDS_motif       complement(1190..1252)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0012"
                     /evalue="1.20E-05"
                     /label="C3_LCL_132-143"
                     /locus_tag="DB708_RS21545"
                     /protein_end="1188"
                     /protein_start="1167"
                     /score="17.9"
                     /tool="antismash"
                     /translation="HHIVSDGVTETLFMHEIGQLY"
     CDS_motif       complement(1448..1558)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0011"
                     /evalue="2.60E-13"
                     /label="C2_DCL_024-062"
                     /locus_tag="DB708_RS21545"
                     /protein_end="1102"
                     /protein_start="1065"
                     /score="42.2"
                     /tool="antismash"
                     /translation="YHNVFPMLIEGPLDIEKLEQALREVVQRHEAFRSAFV"
     aSDomain        complement(1688..1888)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DB708_RS21545_PCP.1"
                     /evalue="4.70E-20"
                     /label="DB708_RS21545_PCP.1"
                     /locus_tag="DB708_RS21545"
                     /protein_end="1022"
                     /protein_start="955"
                     /score="63.6"
                     /tool="antismash"
                     /translation="ERTVAAVWREILGLSVVEVHRSFFEYGGHSLTAAILSSRLEQALE
                     VPVPLREIFEHPTIAALAAQLS"
     aSModule        complement(1688..4738)
                     /complete
                     /domains="nrpspksdomains_DB708_RS21545_Condensation_LCL.1"
                     /domains="nrpspksdomains_DB708_RS21545_AMP-binding.1"
                     /domains="nrpspksdomains_DB708_RS21545_PCP.1"
                     /locus_tags="DB708_RS21545"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1919..1981)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0010"
                     /evalue="3.10E+00"
                     /label="C2_starter_024-063"
                     /locus_tag="DB708_RS21545"
                     /protein_end="945"
                     /protein_start="924"
                     /score="0.6"
                     /tool="antismash"
                     /translation="DVNGKIDRRRLESTVPEVVKE"
     CDS_motif       complement(2186..2251)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0009"
                     /evalue="5.70E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="DB708_RS21545"
                     /protein_end="856"
                     /protein_start="834"
                     /score="34.4"
                     /tool="antismash"
                     /translation="FVGRKDRQVKIRGMRIEPEEIE"
     aSDomain        complement(2216..3376)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DB708_RS21545_AMP-binding.1"
                     /evalue="1.40E-100"
                     /label="DB708_RS21545_AMP-binding.1"
                     /locus_tag="DB708_RS21545"
                     /protein_end="846"
                     /protein_start="459"
                     /score="328.7"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQVARSPNHPAIRYRNETLDYRELDTRANRVANMLLAHGADTG
                     DVIALWSHPSLDTVAGSLGILKAGCVYLPISPDVPAARIAYMLRASKAKLVVAQDLPGE
                     DLDDEIIELGLAARYPGDLRPQLERDPAAIACVLFTSGSTGNPKGTLMRHFNVVRTVVD
                     TNHLAIRPSDRMLQLATPTFDVSMLEVFGALLNGATLVVADSDSRGDLSRIAALLDGER
                     ISVAFMTPALFNAMVDEKLSALGGLRQILTGGDRASADHMRKALARLGRGKLQNGYGPT
                     EATIFTTYYPVDDVAADATNVPIGYPLSNARGAILNGRSPVPVGVVGELCIAGDGLVAG
                     YLDEALTAQKFVIIAGERFYRTGDIAKWHDDGAIEFVGRKDRQVKIR"
     CDS_motif       complement(2324..2410)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0008"
                     /evalue="3.40E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="DB708_RS21545"
                     /protein_end="810"
                     /protein_start="781"
                     /score="38.6"
                     /tool="antismash"
                     /translation="PVGVVGELCIAGDGLVAGYLDEALTAQKF"
     CDS_motif       complement(2915..2974)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0007"
                     /evalue="3.10E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="DB708_RS21545"
                     /protein_end="613"
                     /protein_start="593"
                     /score="25.8"
                     /tool="antismash"
                     /translation="ACVLFTSGSTGNPKGTLMRH"
     CDS_motif       complement(3152..3190)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0006"
                     /evalue="4.10E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="DB708_RS21545"
                     /protein_end="534"
                     /protein_start="521"
                     /score="13.8"
                     /tool="antismash"
                     /translation="LGILKAGCVYLPI"
     CDS_motif       complement(3707..3769)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0005"
                     /evalue="7.70E-08"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="DB708_RS21545"
                     /protein_end="349"
                     /protein_start="328"
                     /score="24.6"
                     /tool="antismash"
                     /translation="LVEKLRPPRDPSRNALYDVLF"
     aSDomain        complement(3860..4738)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DB708_RS21545_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.40E-66"
                     /label="DB708_RS21545_Condensation_LCL.1"
                     /locus_tag="DB708_RS21545"
                     /protein_end="298"
                     /protein_start="5"
                     /score="216.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVCDL"
                     /tool="antismash"
                     /translation="EYPLAPAQQRLWIIHRLNPLSTAYNAPVVRTLRGNVDPRRLEKAL
                     LQLLKRHEQLRATFSLGGAEPTHVIHDVSSFELVYLENVAVKDVSKTITQFIGPFALEH
                     APLLRAALIRVSDHAKLEPSYVLVMDIHHIVCDLRSEDILVRDLFALYHDLPLPEIGSS
                     YTAQLHRQVEPSAAAGQWRADEEFWLSKFAGELPVLQLLDDHRRPAVFDFRGTIRHFPM
                     GNERTKTLRAFATSHQVPLRDIALTAVFVLLAKYSFQEDLVIGMPDEARCCDDMAEVVG
                     MFMNTLAIRANT"
     CDS_motif       complement(3869..3958)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0004"
                     /evalue="1.90E-04"
                     /label="C5_LCL_267-296"
                     /locus_tag="DB708_RS21545"
                     /protein_end="295"
                     /protein_start="265"
                     /score="13.9"
                     /tool="antismash"
                     /translation="DLVIGMPDEARCCDDMAEVVGMFMNTLAIR"
     CDS_motif       complement(4283..4348)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0003"
                     /evalue="3.60E-06"
                     /label="C3_LCL_132-143"
                     /locus_tag="DB708_RS21545"
                     /protein_end="157"
                     /protein_start="135"
                     /score="19.6"
                     /tool="antismash"
                     /translation="IHHIVCDLRSEDILVRDLFALY"
     CDS_motif       complement(4484..4537)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0002"
                     /evalue="4.40E+01"
                     /label="C3_dual_135-156"
                     /locus_tag="DB708_RS21545"
                     /protein_end="90"
                     /protein_start="72"
                     /score="-2.4"
                     /tool="antismash"
                     /translation="HVIHDVSSFELVYLENVA"
     CDS_motif       complement(4562..4675)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21545_0001"
                     /evalue="3.00E-12"
                     /label="C2_LCL_024-062"
                     /locus_tag="DB708_RS21545"
                     /protein_end="64"
                     /protein_start="26"
                     /score="38.9"
                     /tool="antismash"
                     /translation="TAYNAPVVRTLRGNVDPRRLEKALLQLLKRHEQLRATF"
     CDS             complement(4755..>6790)
                     /NRPS_PKS="Domain: PP-binding (156-216). E-value: 1.9e-15.
                     Score: 48.9. Matches aSDomain:
                     nrpspksdomains_DB708_RS21550_PP-binding.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="DB708_RS21550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_43280"
                     /product="AMP-binding enzyme"
                     /protein_id="WP_165839888.1"
                     /sec_met_domain="AMP-binding (E-value: 7.8e-30, bitscore:
                     90.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.8e-15, bitscore:
                     43.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.6e-15, bitscore:
                     44.0, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GYWGDPRLTCQRFVPDPWVAGAVMYKSGDLVKWQESGDLLYVGRV
                     DEQIKLRGFRIEPGEIEARLLEHSLVGQCAVVVSGDRLVAYYTSLEPVDDTALKAHLRD
                     TLPHYMVPDHLVFIEHLPLTRNGKIDRHQLTTHTPTPTPPQPLSRNGTEREEVVHQIFA
                     AVLRVPEVGPDDGFSDLGGNSITAMTLASKLRRAGYNVTARDILHCQTIDKLLKETSEP
                     TIVSAGPRDADLVLQPGMNVDGDVARVLNDSFAINNGIIMGAPVIKDYPMSAMQKLQIG
                     FGTPASLAIEPLERVLDLPALQKAYGAVIAQHGLLRSVPVESGGSHAWREYDYRADEVP
                     LIPVINISECAEPEFSMQALLESLTTRVYKGAKILHQLVIAVVDGNKYFLVWIMSHVIF
                     DRVTKEVLSRQLVRHYDALLQGQPLHTEAPSFEDYVHRLARGPQGVEARDIVRLFRLKE
                     FYEAKQKMKHLVTTTGSPSSTNFNIVLPLSTTWQLEHPWEIALAVHVKGLQRYLQMDQL
                     PLLFVTEGRQFEDQRYYDTVGELTDMIPLFVNARMPLPEITQSILDRLEFVKRHNVNFL
                     HLATEPAAQAQWSDIARLMDVGEDFENLDILMFNILGYTDESSVESVVETSWVHPQPLP
                     IQTLLNAITSVCSETLVCAFRTSCAVDVPRIRACFKDAASELS"
     gene            complement(4755..>6792)
                     /locus_tag="DB708_RS21550"
                     /old_locus_tag="MmonteBS_43280"
     CDS_motif       complement(5832..5903)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21550_0002"
                     /evalue="6.40E-05"
                     /label="C2_LCL_024-062"
                     /locus_tag="DB708_RS21550"
                     /protein_end="319"
                     /protein_start="295"
                     /score="15.4"
                     /tool="antismash"
                     /translation="VLDLPALQKAYGAVIAQHGLLRSV"
     aSDomain        complement(6141..6320)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DB708_RS21550_PP-binding.1"
                     /evalue="1.90E-15"
                     /label="DB708_RS21550_PP-binding.1"
                     /locus_tag="DB708_RS21550"
                     /protein_end="216"
                     /protein_start="156"
                     /score="48.9"
                     /tool="antismash"
                     /translation="VVHQIFAAVLRVPEVGPDDGFSDLGGNSITAMTLASKLRRAGYNV
                     TARDILHCQTIDKLL"
     CDS_motif       complement(6603..6668)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DB708_RS21550_0001"
                     /evalue="1.00E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="DB708_RS21550"
                     /protein_end="62"
                     /protein_start="40"
                     /score="36.7"
                     /tool="antismash"
                     /translation="YVGRVDEQIKLRGFRIEPGEIE"
ORIGIN
        1 aacacacctg cgccccacta tcacgcagaa tcgactcaac ccgcttctgc ggataccccg
       61 ggtcaatcgg aacatacgta gcgcccaccc gcagaatcgc ccaaataccc accaccatct
      121 ccaccgaacg cgccgtcatc aacgccaccg gttcccccgg cctcacaccc aaatccagca
      181 atcgcaccgc catcgactcc gcacgcgcat gcaactgcgc atacgtcaac acctcatccc
      241 gaaaccgcac cgccacccgc cgcgcaaacc tccccacaca cccctcaaac aacccaaaca
      301 acgaaccatc cctcggatac accgacaact cataaacacc aaagccggcg ccggcgggga
      361 gccatgcgag catccgaagc ggcttccccg gatcaagcac gatgtccgac acaaggcgca
      421 ggaacgactc ccccatgcgc aggatggtgc tctcgcgaaa cagcgcagtg gcgtaggtaa
      481 atgacagttg tatctcgtcg gagcactcga acacctcgac cgtcaagtcg accttggcag
      541 acgcgttcgg cagcgaatac ggcgcaatgc gaaggccttc gatttccagc acagcggggc
      601 ccgtcgattg atacgcaaag attgtgtcga acagaggatt tcgtgacgga tcccgttcgc
      661 cttggaacga atggaccatc cgctcgaatg ggtaatcctg atgctcgaag gctgataatg
      721 cttccgcctt cacggagtga aggtaatccg cgaaagccac gccgggcgca gggcgcgttc
      781 tcaaaggcac catgttgacg aacatcccag caatcgactg cacgtcagcg tgcatccggt
      841 tggcgaccgg aattccaact atcagatctt cttgccccga atacttgtgc agcagaacgg
      901 tgtacgccgc gaagagcggc gtgaatagcg tcgtatggtg tgccttcgcc aggtcacgaa
      961 gccgactcgt ctgttcgaca tccagccgaa tcgttaccgt gtgaccgtcg aagttcgtct
     1021 cggaagggcg aggatgatcg agcggcagct ccaacaccgg caacggcccg cgcagccgct
     1081 caacccagtg ttgctcttgc gctcgataac gcccctcctc ccattcgcgc tgctgccaca
     1141 gggtgtagtc cgtgtactgc aagggacgcg gatcgagcgg ctcgtctcga tagagctgac
     1201 ctatctcgtg catgaacagg gtttccgtga caccgtccga gacaatgtga tgattcgcca
     1261 ccacgagcac gtgccgctgg tgacccaggg tcaacaagca ggcgcggaag aggggtggcg
     1321 aggccaggtc gtaagcctcg cgcagtcgtg acacgacggt ggggacatcg gactccgtac
     1381 cttgcgcaca caccagacga aagtcggctc gactcgcaac ggtcttgacc ggaagaccgt
     1441 ctcggagcac gaaagcgctg cgaaacgctt cgtgtcgctg aacgacctcc cgaagggcct
     1501 gttcgagttt ctcgatgtcg agcggtcctt cgatgagcat tggaaaaacg ttgtggtatc
     1561 cgtgatttcg cttcgttgcg tgctggtgtg cgtacaccat gcgctcggca aagctcagcg
     1621 gatagtgcgc gcgtttcggg ttgcgcgcga cgcgggtgcg gcgccgatgc ggggctctag
     1681 acgggccgga tagctgcgcg gccagggcgg cgatcgtggg atgctcgaag atctcacgca
     1741 acgggaccgg tacctctaat gcctgctcta gccgactaga caggatcgcc gcggttaagg
     1801 aatgcccacc atattcgaag aagcttcggt gaacttccac gaccgagaga cccagtattt
     1861 cccgccaaac tgcagccacc gtcctctcga tctcggttat cggcggatgc tcggcttctt
     1921 ccttaacgac ttccgggaca gtcgattcca agcggcggcg atcgatcttg ccgttgacat
     1981 ctaagggaag cgcctccacc acgaataggg agttcggaac catgtagccg ggcaagtgcc
     2041 gcttggcgtc ggcaatgagc tcgtccggcg tgaatgaagc atcgtcgaca cgagtacaaa
     2101 acgcctgcaa gctcttatcg ccctgcgcat ccacccgaac gattactgcc gcctcctgca
     2161 cccacgggtg actttggagc acggcctcga tttcctctgg ctcgatccgc atgccgcgga
     2221 tcttcacttg ccgatccttg cggccgacga attcgatggc gccgtcgtcg tgccacttcg
     2281 cgatgtcgcc ggttcgatag aacctttcgc cggcgatgat tacgaatttc tgtgcggtca
     2341 gagcctcgtc caggtacccc gccacgaggc cgtctcccgc gatgcacagt tcacccacga
     2401 cacccacggg cacaggcgac cggccgttca agatggcgcc ccgagcattc gatagcgggt
     2461 atccgatggg aacgttcgtc gcgtccgctg ccacatcgtc aactgggtag tacgtcgtga
     2521 agatggttgc ttctgtgggg ccgtagccat tctgcaattt gccgcggccg agtcgagcta
     2581 gggctttccg catgtggtcg gcagatgcgc gatcgccgcc agtcagtatt tggcgtaaac
     2641 cacccagagc gctgagcttc tcgtcgacca tggcgttgaa caaggcgggc gtcatgaacg
     2701 cgacggatat gcgttcgccg tcaagcagcg ctgcgatgcg actcaagtcg ccgcgcgagt
     2761 cgctgtccgc taccaccaaa gtcgcgccgt tgagtaacgc gccgaacacc tccagcattg
     2821 atacgtcaaa ggtgggagtc gccagttgga gcatgcggtc cgacggacga attgcgaggt
     2881 ggttggtatc aacgacggtt ctaacgacgt tgaagtgccg catcagggtg ccctttggat
     2941 tgccggtaga accggacgtg aacagcacgc acgcgattgc agccgggtcg cgttcgagtt
     3001 gcggccgcag gtctccggga tatcgcgcgg caaggccgag ctcgatgatc tcgtcatcta
     3061 gatcttcgcc cggaagatcc tgtgcaacaa ctaatttagc cttacttgct cgcaacatat
     3121 acgcgatccg cgctgcgggt acatccggcg agataggcag atatacgcac ccggccttga
     3181 ggatgcccaa cgagccagcc accgtgtcga gtgacggatg cgaccagagc gcgatcacgt
     3241 cgccggtgtc tgcgccatgc gcgagtaaca tgttcgcgac ccggttggcg cgcgtgtcga
     3301 gttccctgta atcgagggtc tcattccgat agcgaatcgc tggatgattc ggtgaccgag
     3361 ccacttgctc ttcgaagaga gcgttgattg tcttccacgg gtatgggctg gccggcgggc
     3421 tgaaggaagc gacgcgattc cgctcatcga ccgagagaat gtcgatgtcc gccagcaggg
     3481 catctggtga actcacgagc gcatcgagca cggcgccata gtgtttcgcc attcgcgtga
     3541 tggtcgggga tcgataaatg gaggtggcga actcgatcga gaaactcagt tgatcgtcta
     3601 tctccagcag cccgaaggtg agatcgacct ctgccttttt atggatgaat tgcctgggcg
     3661 ctacctcgag cccgtcgtaa gtgtctgttt gtaggcattg atgaaagaac aggacatcgt
     3721 aaagcgcgtt gcggctcgga tcccgcggcg gtcgcagctt ctctacgagg taacgcacgt
     3781 cgtacgacga gtgccgtcgc gactcgcaca gatcttcgct gacctcggtc atcaaagtgc
     3841 gaacggtctt gtccggatga gtatttgctc gaatcgccaa ggtgttcatg aacatgccga
     3901 caacctctgc catgtcatcg caacaccggg cctcgtctgg catgccgata acgaggtcct
     3961 cttgaaagct gtattttgcc aagagaacaa atacggccgt cagagctatg tcacgcaatg
     4021 gcacttgatg tgatgtcgcg aaggcacgca acgtcttggt ccgttcgttg cccatcggaa
     4081 agtgccgaat cgttccgcga aagtcgaaga cagcgggccg tcggtgatcg tctagcagtt
     4141 gcaagactgg aagttcgccc gcaaacttcg agagccagaa ttcctcgtcc gcccgccatt
     4201 ggccggcggc cgccgagggc tccacctgcc tgtgtagctg cgcggtgtat gagctgccga
     4261 tctccggtag gggtagatcg tgatacagcg caaacagatc ccgaacgagg atgtcctccg
     4321 accgaaggtc gcagacgatg tgatggatgt ccatcacgag gacgtacgag ggttcgagct
     4381 ttgcgtgatc ggacacacgg ataagagccg ctctcagtaa gggtgcgtgc tccagcgcga
     4441 acggcccgat gaattgggtg attgtcttcg aaacgtcttt cactgctacg ttttcgaggt
     4501 agaccaactc gaacgacgaa acgtcgtgaa tcacgtgcgt cggctcggcg ccccccagcg
     4561 aaaaagtcgc acggagttgt tcgtgacgtt tcaacaactg caggagtgcc ttttctagcc
     4621 gccgggggtc gacattgccg cgcagagtcc gaacgacggg tgcgttgtag gcggtgctca
     4681 agggattcag tcggtggatg atccacaatc gctgttgcgc aggcgcgagg ggatactcgt
     4741 cgatcgtcgc cacgttaaga caactccgat gcggcgtcct tgaagcatgc tcgaattcgg
     4801 gggacgtcca ctgcacaact cgtacgaaat gcgcaaacca atgtctcgga gcacacgctg
     4861 gtgatggcat tcaggagggt ttgaataggc aggggctgcg ggtgcaccca gcttgtctcc
     4921 acgacggact ccacagagga ttcatccgtg taaccgagga tgttgaacat caatatgtcg
     4981 agattttcga aatcttcgcc gacatccatg agtcgtgcga tgtcggacca ttgggcctgt
     5041 gcggccggtt ccgttgccag gtgaagaaag ttcacgttgt gccgcttcac gaactcgagt
     5101 cgatcgagga ttgattgtgt gatctccggt aggggcattc gtgcattcac gaatagaggg
     5161 atcatgtcgg tgagttcgcc cacggtgtcg taatatcgct ggtcttcgaa ctggcgaccc
     5221 tccgtcacga acaacagcgg cagctggtcc atctgcagat agcgttgaag gcccttcacg
     5281 tgaacggcga gcgcgatctc ccaagggtgc tccagttgcc acgtagtgct caatggcagc
     5341 acgatgttga agttggtgga tgaaggcgat cccgttgtcg ttaccagatg cttcatcttt
     5401 tgtttcgctt cgtagaattc tttgagtcgg aacaaccgca cgatgtcgcg tgcttccacc
     5461 ccttgcggac cacgagccag gcggtgcaca tagtcctcga atgatggcgc ctcggtgtgt
     5521 aacggttgcc cttgtagcaa ggcatcgtaa tgccgaacca actgtcgcga caggacctcc
     5581 ttggtaaccc tgtcgaagat gacatggctc atgatccaga ccaggaagta tttgtttccg
     5641 tcgaccactg ctatgaccaa ttgatggagt attttcgcac ccttgtatac ccgcgtggtg
     5701 aggctctcga gcagcgcttg catcgagaat tcaggctcgg cgcactcgct gatgttgatc
     5761 accgggatca gcggcacctc gtccgcgcgg tagtcgtatt cgcgccacgc atgacttccg
     5821 cccgattcaa ccggcacgct ccgaagtaac ccgtgctgcg cgatcacagc tccgtaggcc
     5881 ttctggaggg cgggaagatc taatacgcgt tctagtggct ctatggctaa cgaggcggga
     5941 gtcccgaagc caatttgcaa tttttgcatt gcgctcattg ggtaatcttt aatcacgggt
     6001 gcgcccatga tgattccgtt attgatggcg aacgagtcgt tcagaacgcg cgcgacgtca
     6061 ccgtcgacat tcatcccggg ctgcaagacc aaatcggcgt ctcgtgggcc ggctgaaacg
     6121 atggttggct cggatgtctc tttcagcagc ttgtcgatgg tctgacagtg caggatgtcc
     6181 cgcgccgtca cgttgtaccc cgctcgccgc aatttcgaag ccaaggtcat cgcggtgatc
     6241 gagttgcccc ccaggtctga gaaaccgtcg tccggtccca cctcgggcac acggagtacc
     6301 gccgcgaaaa tctgatgcac cacctcctca cgctctgtgc catttctcga caaaggttgg
     6361 ggtggtgttg gtgtgggggt gtgtgtggtg agttggtggc ggtcgatttt tccgttgcga
     6421 gtcagtggta gatgctcgat gaagaccagg tgatcgggga ccatgtaatg cggcagcgtg
     6481 tcacgcaggt gagcctttag tgccgtgtca tcaacaggtt caagcgacgt gtaataagcg
     6541 accagacgat caccggacac caccacagcg cactgcccaa caagtgagtg ctcaagcagt
     6601 cgcgcttcga tctcaccggg ctcgatacga aatcccctga gcttgatctg ctcatcaaca
     6661 cgcccgacgt agagcaggtc cccgctttcc tgccacttca ccagatcccc ggacttgtac
     6721 atgacagcac cagcaaccca cgggtcgggc acaaaccgct gacaagtaag ccggggatcc
     6781 ccccaatacc cc
//
