LOCUS       NZ_BFCH01000011        11416 bp    DNA     linear   CON 22-DEC-2024
DEFINITION  Mycobacterium montefiorense strain BS sequence11, whole genome
            shotgun sequence.
ACCESSION   NZ_BFCH01000011
VERSION     NZ_BFCH01000011.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Mycobacterium montefiorense
  ORGANISM  Mycobacterium montefiorense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium; Mycobacterium simiae complex.
REFERENCE   1
  AUTHORS   Fukano,H., Yoshida,M., Shimizu,A., Iwao,H., Katayama,Y., Omatsu,T.,
            Mizutani,T., Kurata,O., Wada,S. and Hoshino,Y.
  TITLE     Draft Genome Sequence of Mycobacterium montefiorense Isolated from
            Japanese Black Salamander (Hynobius nigrescens)
  JOURNAL   Genome Announc 6 (21), e00448-18 (2018)
   PUBMED   29798927
  REMARK    DOI:10.1128/genomeA.00448-18 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325462)
  AUTHORS   Fukano,H., Yoshida,M. and Hoshino,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2018) Contact:Yoshihiko Hoshino National Institute
            of Infectious Diseases, Mycobacteriology; 4-2-1, Higashimurayama,
            Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Platanus v. 1.1.0
            Genome Coverage                   :: 129x
            Sequencing Technology             :: Illumina Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003112775.1-RS_2024_12_22
            Annotation Date                   :: 12/22/2024 07:39:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,429
            CDSs (total)                      :: 5,376
            Genes (coding)                    :: 5,180
            CDSs (with protein)               :: 5,180
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 91 of 196
            Pseudo Genes (incomplete)         :: 88 of 196
            Pseudo Genes (internal stop)      :: 32 of 196
            Pseudo Genes (multiple problems)  :: 14 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:34:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 44826
            Orig. end                         :: 56242
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BFCH01000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11416
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11416
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11416
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6416](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6416
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(750..1910)
                     /gene="ripC"
                     /locus_tag="DB708_RS07815"
                     /old_locus_tag="MmonteBS_15680"
     CDS             complement(750..1910)
                     /codon_start=1
                     /gene="ripC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729681.1"
                     /locus_tag="DB708_RS07815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_15680"
                     /product="peptidoglycan hydrolase RipC"
                     /protein_id="WP_108921389.1"
                     /transl_table=11
                     /translation="MSSGSAHSISRVITRSVVGTLACLTVLSGVCAANVMADPADDALA
                     KLTELSRQAEQTTEAMHSAQLDLNDKLAAQRAADKKHTDDHAAVDGAKVRLATFQIAVN
                     KLAAATYMGGRIDGMEAMLTAQSPQGLIDKIAVQRVMAAQMSTQMASFRVAGEQAAKAE
                     QASARSAADAKTAAEQAAAVRASLQSKQSQLQVQIAVVKSQYVSLSPQQRTALADPGQL
                     PAAAPAPGAPAPDALPPGAPPAPGAPPPGEAPPPGGMPGMPGMIPGGPGGDRANVVQAA
                     LTQVGSPYVWGGSAPGGFDCSGLVMWAFQQAGISLPHSSQALAHGGQPISLSDLQPGDV
                     LTFYSDASHAGIYVGDGMMIHSSTYGVPVRVVPMNSSGPIYDARRY"
     gene            complement(2097..2351)
                     /locus_tag="DB708_RS07820"
                     /old_locus_tag="MmonteBS_15690"
     CDS             complement(2097..2351)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394895.1"
                     /locus_tag="DB708_RS07820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_15690"
                     /product="hypothetical protein"
                     /protein_id="WP_108921390.1"
                     /transl_table=11
                     /translation="MAYSSGPDSKGVAASVPGAPVVIDCDDCAARGPGCRDCVISVLLG
                     VPDTLLHDERAALEVLAEVGLAPRLRLVPIHRNHGSGVA"
     gene            2466..4364
                     /locus_tag="DB708_RS07825"
                     /old_locus_tag="MmonteBS_15700"
     CDS             2466..4364
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1194: DNA polymerase
                     III subunit alpha"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787107.1"
                     /locus_tag="DB708_RS07825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_15700"
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="WP_108921606.1"
                     /transl_table=11
                     /translation="MLNVTPMGVTGGTQLNFADLGSSAESSWTPLEETPLRETTFVVVD
                     LETTGGRARGGDGTRADAITEIGAVKVRGGVVLGEFGTLVDPQRSIPPQIIQLTGITTA
                     MVCDAPTIDAVLPMFLEFAGLDCGAVLVAHNAGFDVGFLRAAAEHCEIRWPQARVLCTV
                     RLARRVLSREESPSVRLAALARLFAVATQPTHRALDDARATVEVLHTLVERVGNQGVHT
                     YADLRSYLPNVTPTQRRKRVLADQLPHRPGVYLFRGPSGEVLYVGTAVDLRRRVSQYFT
                     GADPRGRMKEMVTLAGAVDHVECAHALEAGVRELRMLAAHAPPYNRRSRFPQRWWWVAL
                     SDEAFPRLSVVRAPRHDRAIGPFRSSADAADTARLLARFTGIRTCTTRLGRSALHGPAC
                     PEVEVSPCPAARDVTAVQYAAATQRAAALIDGADNSALIAAVDQITVLAEHRHYESAAR
                     LRDRTATAVEMLWRGQRLRALAALPELIAAAPDGSGGYHLAVIRYGQLAAAGNARRGVP
                     PMPVVDAIHAGAQTILTASAPLGGALVEETALVARWLLTPGVRIVRVADGVGWASPVRS
                     AGAWAGWAAAARSARLARTQDPAPVPDSDLPAVHYTITQRSTGRPNAEIQCSPKGA"
     misc_feature    complement(4580..4589)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            5001..6416
                     /locus_tag="DB708_RS07830"
     CDS             5001..6416
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.5"
                     /locus_tag="DB708_RS07830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_108921391.1"
                     /sec_met_domain="YcaO (E-value: 4.8e-38, bitscore: 125.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDSVLQIRSSLSDFTYFQRDTGPRGNPSKKALQRKKAPLFGPLC
                     QPYERELSLSEAQHRIDLELAELGWRVEVGGALNDALSAATAVLTDRSGRKVQDGRGAG
                     KGMPLNSRIGAIFEALEHVYSGPILTGHLDTHTIDVSKLAAGPLAHDPAIIELAAGIAA
                     DTPIACLKYCFAHIHGGSAISVTIFLWAPWFTSVAADLWRQSIGDGVDYSRIKSYSVNT
                     GCAIGASEDEAVLHALNEWVERDALSVFLLTSIYDSTTPPRRISRQILSPSSVKLLTRA
                     EKTIGKAIDLFDITTDIEVPAVLATVSGESDLNSPHYGLGVSISADHALERAVTEVVQA
                     VLLAPLAAQETPPIDLSQIFRGHPQLLACARMQIPESVVDLPSTARRFSAREDPRAIKA
                     QRHQVTKRIAAAGHDILIRRLATLHHGTTIVQVQCPGLERFHLVTNGHVTIPGQRANRL
                     RKSFNCPGRRLRS"
     misc_feature    complement(6478..6494)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.32"
                     /tool="antismash"
     aSDomain        6847..7227
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DB708_RS07835_Abhydrolase_1.1"
                     /evalue="2.70E-24"
                     /label="DB708_RS07835_Abhydrolase_1.1"
                     /locus_tag="DB708_RS07835"
                     /protein_end="127"
                     /protein_start="0"
                     /score="78.0"
                     /tool="antismash"
                     /translation="MVIVHGGPGGRSGPRHRNLVDLTRWRVIQYDQRGCGNSTPRGQYS
                     HNTFDDLVDDLERLRLHLRVRSWVVLGFSWGTTIALAWASRYGSSGHALILSGVYLGRR
                     CDLAIGRPHPQVPFAAWKDVVAN"
     gene            6847..7674
                     /locus_tag="DB708_RS07835"
     CDS             6847..7674
                     /NRPS_PKS="Domain: Abhydrolase_1 (0-127). E-value: 2.7e-24.
                     Score: 78.0. Matches aSDomain:
                     nrpspksdomains_DB708_RS07835_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024109.5"
                     /locus_tag="DB708_RS07835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_165839761.1"
                     /sec_met_domain="PF00561 (E-value: 7e-24, bitscore: 79.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 5e-11, bitscore:
                     37.9, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVIVHGGPGGRSGPRHRNLVDLTRWRVIQYDQRGCGNSTPRGQYS
                     HNTFDDLVDDLERLRLHLRVRSWVVLGFSWGTTIALAWASRYGSSGHALILSGVYLGRR
                     CDLAIGRPHPQVPFAAWKDVVANIAENERNRVAAAYLSRFRDRTHPDHRGAIRRWIALD
                     GAVSGLPYAPDTIVVEPGMGESLSIEAHYVAHDFFLPVDGVIPLIKSIPTAMQIHVAQG
                     LNDSACRESAIALNMARTIKLTWVLAGHSVLNPKLTSAVFDSTEYVRLTMDCL"
     gene            7896..8612
                     /locus_tag="DB708_RS07840"
                     /old_locus_tag="MmonteBS_15720"
     CDS             7896..8612
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025033.5"
                     /locus_tag="DB708_RS07840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_15720"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_165839762.1"
                     /transl_table=11
                     /translation="MIAGIYLGSQVDNCGVTFHYRAAHKAERLNIFRGADPLATEIIFP
                     YPLLDNAGNEYVSPSGEWDENLTLINGLDADELHLREHGFNLAEKFLVPGSVIYDPACS
                     TGTYLGSLSRYFPNCNFIGSDRSFAMTQRAAEKIPRVFVADAGNAPIANGSIDLLILRF
                     LNAEVMPRTIAGDLFIQLSNLVSPRGRIFMFGHTPLLLDVSSAAHAANLQIESQIGVEP
                     RSAGIFQFYQLGRGAT"
     gene            8660..9874
                     /locus_tag="DB708_RS07845"
                     /old_locus_tag="MmonteBS_15730"
     CDS             8660..9874
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005076026.1"
                     /locus_tag="DB708_RS07845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_15730"
                     /product="MFS transporter"
                     /protein_id="WP_108921394.1"
                     /transl_table=11
                     /translation="MRHRDYLLLTVGLAVTLLGAGMSTVALFWQIIAMGGGPAQVSLVM
                     TVCGLGLLATLLPAGVAADRLPRLWVMRVSLVAQAILLLVLGWLTVTDRVQIWHFGLAG
                     LILGIAEGMYFPSYTALLPSLLPAEDLLSANGTGGVLRPGLQLAAGPALGAFVVKLWST
                     GSAFLTQGVLMVVAVLMLSLMRTGSSKPILDGENRGTAVADLLEGARYMVRTSWFFATL
                     LFANGYVLVVIGPIEVLVPFILRDLGGGPGTQAMVLAIFGIAGAVGAITVSSLFLARRY
                     LTVMLAMWGLGSLPLIVVGQTHSIWIIVASSVVVGATGQAAQVIWGTLLQRRMPPEMLG
                     RASSLNFFVGLVMMPASYALAVPAAQLMGVAGVFVIAAVTPAVLSVIVHVAARLGRDEI
                     AHSLQ"
     gene            complement(10052..11170)
                     /gene="trpD"
                     /locus_tag="DB708_RS07850"
                     /old_locus_tag="MmonteBS_15740"
     CDS             complement(10052..11170)
                     /EC_number="2.4.2.18"
                     /GO_function="GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216708.1"
                     /locus_tag="DB708_RS07850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_15740"
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="WP_108921395.1"
                     /transl_table=11
                     /translation="MALSSEVPSPRAFSATSPGAKAGIAPTWPRLLGRLTDGENLARGQ
                     AAWAMDQIMTGNARPAQIAAFAVAMKMKVPTAAEVSELADVMLSHARQIPGDAIGDDAV
                     DVVGTGGDGVNTVNLSTMAAIVVAAAGVPVVKHGNRAASSLSGGADTLEALGVRIDLGP
                     DQVARSLAEVGIGFCFAPVFHPSYRHASAVRREIGIPTVFNLLGPLTNPARPRAGLIGC
                     AFANLAEVMAGVFAARRASVLVVHGDDGLDELTTTTTSTIWRVQAGTVDKLTLDPAGFG
                     FARADLDELVGGDAAANAEEVRAVLGGAKGAVRDAVVLNAAGAIVAHAGLSSRAEWLPA
                     WEDGLERAGAAIDSGAAEQLLARWVRFGQQVA"
     misc_feature    complement(11402..11415)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.74"
                     /tool="antismash"
ORIGIN
        1 tgtcggcgac gaagcgggcg aaccaccagg cgcggtcgta ggctagcgaa cgctgcggcc
       61 ccggggtgtc cagctcgaga tccgacggca acgacgggtt cggcgagcga gcctgcggcg
      121 gcaccggggt attgggccgg gccacgaaat cggcgacgcc ttcggctagc catcgcggcg
      181 cgtcgagcgc ggtgtcggcg cgcgatgcgt aatgaaaaag ttcatgagtc aacacaattc
      241 gaaatgccga cgcactcatt cctgccgcgc ccggggcgaa tacgatccgc tgaccgacca
      301 ccacccgatg ggcgggatca atctgatcgg cgacggtcac cgcggcgatg tccgcccatt
      361 gctcgggtgc gccgccgccg gcggcggcgc gaaactggtc gtcggagccg gtggccacca
      421 ccgagatatc ccgcgaccag ccgacccccc agaacgcctc caccttgtcg accgcggtgc
      481 ccatgtccga cccgacccgg gtcagcagct ggtcggcggc cgggccgccg aggctgagca
      541 atcggatctc gcggtcgccg acgatcaggg gtgctcgcgc gccgatcggg tggctatggg
      601 cgggcgcaac cagctgcgcg cctcttagcc ggccattgcg tgcgtccaac ggcacggccg
      661 ctgccgcgac gaactcgaca acaaagaccc acgccaaaag aatcgggagc caccggcgat
      721 gccgacggaa atggccgggc gccgaacgat cagtaacggc gggcgtcgta aatcgggccg
      781 gaggagttca tcggcaccac ccgcaccggc acgccgtagg tggacgagtg gatcatcatg
      841 ccgtcaccga cgtatatgcc ggcgtgcgac gcgtccgaat agaaggtcag cacgtcgccg
      901 ggctgcagat ccgacaggga aatcggctga ccgccatgag cgagcgcctg gcttgagtgc
      961 ggcaacgaga tgccggcctg ctggaaggcc cacatcacca atccggagca gtcaaacccg
     1021 cccggtgccg agccgcccca cacgtaaggc gatccgacct gggtcaacgc cgcttgcacg
     1081 acgttggcac ggtcaccacc gggaccgcca ggaatcatcc ccggcatacc gggcattcca
     1141 cccggcggcg gagcctcacc cggcggtggc gcaccgggcg ccggcggggc gccgggcggc
     1201 aatgcgtccg gcgcgggtgc gcctggcgcc ggagccgccg cgggcagctg cccggggtcg
     1261 gccagcgcgg tgcgctgctg tggcgacagg gacacgtact gcgacttcac gacggcaatc
     1321 tgaacctgca gctggctctg cttggactgc aggctggccc gcaccgcggc ggcctgctcg
     1381 gccgcggtct tggcatcagc ggcggagcgg gccgaagcct gttcggcctt ggcggcctgc
     1441 tcaccggcaa cccggaaact tgccatttgc gtggacattt gggccgccat cacgcgctgg
     1501 acggcgatct tgtcgattag cccctgcggc gactgcgcgg tcagcatggc ttccatgccg
     1561 tcgatgcgtc cacccatgta ggtcgcggcc gcgagcttgt tgacggcgat ctggaaggtg
     1621 gccaggcgaa ccttcgcccc atccaccgcg gcgtggtcgt cggtgtgctt cttgtcggcg
     1681 gcccgctggg cggcgagttt gtcgttgagg tcgagctgcg cgctgtgcat ggcctcggtg
     1741 gtctgctcgg cctgccgaga tagctcggtg agctttgcca gtgcgtcgtc ggcgggatcg
     1801 gccatcacgt tggccgcaca gacaccggac aaaacggtga ggcatgccag cgttccgacg
     1861 actgaccgcg tgatgacacg cgagatcgaa tgcgcggagc cggaactcaa agtattgctt
     1921 ccttaaactg ccgtcgacgt tcgagatcga tctgggttta ggtctcaaat aggttacgaa
     1981 acgatatcgg cgtttgtcca aaccagatcg ttaagaaaat agatagattt ctgtcataag
     2041 tatgtgaacg acacggggta actgaggcgg tccaggggtt ttgacactca acccctctag
     2101 gcgacgccgg acccatgatt gcgatgaatg ggcaccaaac gcaagcgcgg cgccaatccc
     2161 acctccgcga gcacttcgag tgcggcacgt tcgtcgtgca acaaggtatc cggtaccccg
     2221 agtagcacgc tgatcacgca atcccggcac cccgggccgc gagccgcaca atcgtcgcag
     2281 tcgatcacca ccggggcgcc cggtactgac gccgcgacgc ctttgctgtc gggtccgctg
     2341 ctgtaggcca tcggaatcgg tcctctcatc cgcacttggt cgtgctgacc cgaacgctaa
     2401 ccgtgggcac cgacagtttt tggcgagcgc gaaccggcgt gggaacgccg ccggtgtcgg
     2461 tcgcgatgct taacgtcacg cccatgggtg tgactggtgg gactcaactg aatttcgccg
     2521 acctgggatc cagcgcggaa tcgagctgga cgccactcga agaaaccccg ctgcgcgaga
     2581 ccaccttcgt cgtggtggac ctggagacga ccggcgggcg cgcccgcggc ggcgacggga
     2641 cacgtgccga cgcgatcacc gagatcggcg cggtcaaggt tcgcggcggc gtcgtgctcg
     2701 gagaattcgg caccctggtg gacccgcagc gcagcatccc gcctcagatc atccagctca
     2761 ccggcatcac cacggccatg gtgtgtgacg ccccgacgat cgacgcggtg ctgccgatgt
     2821 ttctggagtt cgccggcttg gactgcgggg cggtgctcgt cgcccacaac gcggggttcg
     2881 acgtcgggtt tctgcgggcc gccgccgagc actgcgaaat tcgttggccc caggcacgag
     2941 tgctgtgcac cgtacgactg gcgcgccgcg tgctgagccg tgaggaatcc cccagtgtgc
     3001 ggctggcggc cctggcccgg ctgttcgccg tcgccaccca gcccacccac cgggccctcg
     3061 acgacgcccg cgccaccgtc gaggtgctgc acacactggt cgagcgcgtc ggcaatcagg
     3121 gcgtgcacac ctatgccgac ctgcgctcgt atctgcccaa cgtgacgccg acccagcgcc
     3181 gcaagcgtgt gctcgccgat cagctgccgc accgtcccgg ggtctacctg tttcgcggcc
     3241 cctcgggcga ggtgctctac gtcggcaccg ctgttgacct gcgccggagg gtgagccagt
     3301 acttcaccgg cgccgaccct cgcggccgga tgaaggagat ggtcacactg gccggcgcgg
     3361 tcgaccacgt cgagtgcgcg cacgcgctcg aggccggggt gcgcgagctg cggatgctgg
     3421 ccgcgcacgc gccgccgtac aaccgccggt cgaggttccc gcagcgctgg tggtgggtgg
     3481 cgctgagcga cgaggccttc ccccggctgt cggtggtgcg cgccccgcga cacgaccgcg
     3541 ccatcgggcc gtttcggtcc agcgccgatg cggccgacac cgcgaggctg ctggcacgtt
     3601 tcaccggaat ccgaacgtgc acaacacgac tcggacgttc ggcgctgcac ggaccggcct
     3661 gcccggaagt cgaagtgtcg ccctgtcccg ccgcccgcga cgtgaccgcg gtgcaatacg
     3721 ccgcggcgac acagcgggcc gcggcgctga tcgacggcgc ggacaacagc gcgttaatcg
     3781 cggccgtcga tcagatcacc gtgctggccg agcatcggca ctacgagagc gcagcacggc
     3841 tgcgcgaccg caccgccacc gccgtcgaga tgctgtggcg gggccagcgg ctgcgggcgc
     3901 tggccgcact gcccgagctg atcgccgcgg cccccgacgg cagtgggggc taccacctcg
     3961 ccgtgatccg ctacggccag ctcgccgcgg ccggcaacgc caggcgcggg gtgccgccga
     4021 tgccggtggt cgacgccatc catgctggcg cacaaacgat tttgacggcc agtgccccgc
     4081 tgggcggtgc gctggtcgaa gagaccgccc tggtcgcgcg ctggctgctg accccgggag
     4141 tgcgcatcgt gcgggtcgcc gacggcgtcg ggtgggcctc accggtgcga tccgccggcg
     4201 cctgggcggg gtgggcggcc gcggcgcgct cggcgcggct ggcccgcaca caggatccag
     4261 cgccggtccc ggactcggac ctgccggcag tgcactacac gattacgcaa cggagcaccg
     4321 ggcgccctaa cgctgagatc caatgctccc cgaagggagc ctgaccgata cccatccgct
     4381 tcggccgcgt actaccagcc gaagccgaac agatcgcgga tcaacctata ccgcaacaac
     4441 tttcattaat cgacgcaccc agatcagagt tcgtcaatcg ccgggccggg acaaacgaga
     4501 tgtttcctca atggacacag gtgtaaacga tatgtttcct cactagataa atgaaactct
     4561 aaatatagac aagtgaaact caaaaaatat agacaaatga gactcaaaat tacgagcggt
     4621 tgacggcata catgccgcct gactacccta cgttaggtat ccattcacat ggttacgtca
     4681 gagcgaatta tggtgctctt tggaaatttc cgattcatct cgcaagatca atcaggagga
     4741 tttacccatg ggatatttag ctgttttcaa cgccgcggat gtcgacgatt cggctatctc
     4801 tgcagagata gccgctacaa tcgaccgaat tgacaccgcc gctgaggatt ccgtctaatc
     4861 ctaaactact ggtcgccggg ccgtaatcaa ccacaattgt ggcccggcga ccgggtcaat
     4921 tcgcatcgga ccaacactgc agatttgaat accgggggct gcgttgatcc ctcacacctc
     4981 aagaacgctc catttagtag atgcctgata gcgttctaca gatacgatcg agtttgtcag
     5041 atttcacata ttttcagcgc gatactggtc ctcgcggaaa tccatcgaag aaggcactgc
     5101 aaagaaagaa ggcacctctg ttcggacctc tttgccagcc atacgagcgg gaattatccc
     5161 tgtccgaagc gcaacatcga atagatttgg agcttgcgga gctgggatgg agagttgagg
     5221 taggcggtgc gctaaacgat gcgctttctg ctgcaactgc agtgctcacc gatagatctg
     5281 gccgaaaggt acaagatggg cgcggcgcag gtaaaggaat gcctttgaat tcgagaatcg
     5341 gtgcgatatt cgaagcgctg gaacatgtat attctgggcc gattcttacg ggacatctag
     5401 acactcatac cattgacgtc tccaaattgg ctgctgggcc actcgctcat gacccggcta
     5461 tcattgaact ggcagcgggg atcgctgccg atactccgat cgcgtgcttg aaatactgct
     5521 tcgcccacat acacggtggc agtgcgatca gtgttacaat atttctttgg gcaccgtggt
     5581 tcacatcggt agcggccgat ctgtggcgtc aaagtattgg agacggggtc gattactcgc
     5641 gaatcaaatc atattcagtg aatactggtt gcgcgatcgg cgccagcgaa gatgaggcag
     5701 tactgcatgc actaaacgag tgggtcgagc gggacgcact ttcggtgttt ctacttacca
     5761 gtatttacga ttccactacc ccgccacggc gcattagccg tcagattctg agcccgtcaa
     5821 gtgtcaagtt gttgactcgt gccgagaaaa cgatcggaaa ggctatcgat ttatttgata
     5881 tcacaacaga tatagaggta cctgcagttc ttgcgacggt atctggagaa tctgatctca
     5941 acagtcctca ctacggactt ggggtgtcga tttcagccga tcatgcattg gaacgtgcgg
     6001 tcaccgaggt cgtacaggcg gttcttctag cgcctttagc agctcaagaa acaccaccga
     6061 ttgatcttag ccaaatcttt cgtggtcacc cccagttact ggcgtgcgca cgtatgcaaa
     6121 tccctgagag tgtcgtcgac ctgccgagca ctgcgcgccg attctcggcg cgagaagatc
     6181 cccgagccat aaaagcacag cgtcatcaag taaccaagcg catcgccgcc gccggtcacg
     6241 acatccttat tcggcgactg gcaacactgc atcatggcac caccattgtc caagtgcagt
     6301 gcccaggctt ggaaaggttc cacctggtaa ccaacggtca cgtcacaatt cctggtcaac
     6361 gagccaatcg gttaagaaag tcctttaatt gtccagggcg tcggttacgt tcgtgatttt
     6421 cggctttagc ctggagtcgg tttggtggac cgccagcacc cggcgagaat attcggattc
     6481 aagcgaaatt acagatgtgg agtgcccaag gggcgcggaa gcccctcaca cctcaagaac
     6541 gctccattta gcagatactg acagcgttct acacatgacg atcaaatttg tcagaccgta
     6601 ctaccgctat gaacatccct gcgatattgg taaatgtgta tccgtagcta atggtaatgt
     6661 ttaggtgtgg cgtgaacgcc gtcgtaacag caccaagatc gaagggttcg tacccgcgat
     6721 ttgatgggct aaaaaatgag taatctagat gtgttgtgcg ccgtcagtcg tagttccggt
     6781 tggctagatg tcggtaatca gcataggatt agatggtggg atagcggaat cggcgacgga
     6841 ccaactatgg taatcgtgca tggaggtccg ggagggcgta gtgggcctcg acatcgcaat
     6901 ctggtcgact taacgcgatg gcgtgtaatt caatacgacc agcgtggatg tggcaactcg
     6961 actcctcgcg gtcagtacag ccacaatact ttcgacgatc tggttgatga ccttgaacgg
     7021 cttcgcctac atctgcgggt acgaagttgg gttgtcttag gcttttcgtg ggggacaacg
     7081 attgccttgg catgggcatc tcgatacggt agttctggtc acgcgctaat actgagtgga
     7141 gtctatctcg gtcgtagatg cgacctagca atagggcgtc cgcatcccca ggtgcccttc
     7201 gccgcatgga aagatgtagt ggccaacatc gccgagaatg agcggaatcg ggtggcagct
     7261 gcttatttat ccagattccg ggaccgtact cacccagacc accgtggtgc catccgacgc
     7321 tggatcgcac ttgatggcgc ggtcagcgga ttgccctacg cacccgatac catcgttgta
     7381 gaaccaggaa tgggcgaatc gctgtcgatc gaagcacatt acgtggccca cgactttttt
     7441 ttaccagttg acggagttat tccgctcatt aaatcgatac ctactgctat gcagatccat
     7501 gtagcacagg ggttgaatga tagcgcgtgc cgcgagagtg ctatcgcact gaacatggcg
     7561 cggaccatca aattgacctg ggttttggcg ggacattcgg tcttaaaccc gaaactgact
     7621 tccgctgtat ttgacagcac tgaatatgtc cgtttaacaa tggactgttt atgatcgctc
     7681 attcgaattc gcgtcaaatc gaggtcgaca cgtataacca agtgcatact accgggttcg
     7741 gtgcggccgg taaggtatgt tcgtggcttg agtccacccc tgcgtgtcta aatcaatatt
     7801 tcctgcttcg tcatcgagat gaaacgtggt tgggaaggaa gatactcact cacgattcga
     7861 gaggctatag gatttctagc attctagctc cccatatgat cgctggaata taccttggaa
     7921 gtcaggttga caactgtggt gtcactttcc actatcgcgc agcccataaa gcggaacgct
     7981 tgaatatatt taggggcgca gatcctctcg cgaccgagat tatttttcct tatccgctcc
     8041 tcgacaatgc cggaaacgaa tacgttagcc cttcgggcga atgggacgaa aatcttactc
     8101 ttataaacgg tttagacgcc gatgaactgc atttgcgcga gcatggtttt aatctagccg
     8161 agaagttcct cgttccgggt tcggttattt atgatccggc ctgctcgaca ggtacctacc
     8221 tgggctcgct tagtcgatac ttccctaact gtaatttcat tgggtcagat cgatcgtttg
     8281 cgatgacgca gcgtgctgcg gagaaaattc cacgtgtatt cgttgccgat gctggcaatg
     8341 cccctatcgc gaacggtagt atcgatttgc ttattctgag atttcttaac gcggaagtaa
     8401 tgcctaggac gattgccggt gacctattta tccagctatc gaacctcgtg tctccgaggg
     8461 gccgtatatt tatgttcggc cacactccgc tgctgctgga cgtttcctcg gcggctcacg
     8521 cagctaattt gcagattgaa agccaaattg gtgtagaacc ccgctcagca ggaatttttc
     8581 aattttacca actgggtcgc ggtgctacgt gacaaccgtc gacaatcaag ggatacgtct
     8641 gcctagggta ctgcgcccat tgcggcaccg tgactatctg ctgctgactg tggggctcgc
     8701 ggttacactg ctgggcgccg ggatgtcgac ggtggcgctg ttctggcaga tcatcgccat
     8761 gggaggtggg ccggcgcagg tctccctcgt gatgaccgtg tgcggtcttg ggcttttggc
     8821 cacactgctg ccggccggtg tcgctgccga tcggctgccc cggctgtggg tgatgcgggt
     8881 ttcgcttgtg gcgcaagcga ttctactact ggtgctcggc tggctaacgg tgaccgatcg
     8941 ggtccagatc tggcactttg ggttggccgg tctgatcctt gggatcgccg aaggcatgta
     9001 cttcccttcg tacacagccc tgctgccatc gctgctccca gccgaggacc tgttatcggc
     9061 caatgggact gggggcgtat tacgaccggg gctacagctc gccgccgggc cagcgcttgg
     9121 tgctttcgta gtgaagctct ggtcgaccgg cagcgccttc ctgacacaag gcgtgttgat
     9181 ggtcgtcgcg gtgctgatgc tgtcgttgat gcgcaccggt tcctcgaagc cgattctcga
     9241 tggtgaaaat cgcggaacgg cggtggcgga cctgctggaa ggcgcgcgct acatggtgcg
     9301 caccagctgg ttcttcgcca ccctgctttt cgccaatggt tacgtgttgg tcgtgatcgg
     9361 acccattgag gtgctggtgc ccttcatact tcgcgacctg ggcggtggtc ctggcaccca
     9421 ggctatggtg ctggcgatct tcggcatcgc cggggcggtc ggggcgatca cggtgtcctc
     9481 gctgtttctc gcgcggcgct acctgacggt gatgctggcc atgtgggggt tgggttcgtt
     9541 accgctcatc gtggtggggc agacccactc gatctggatc attgtggcgt ccagtgtggt
     9601 ggtcggcgct accggtcagg ccgctcaggt gatctggggc acgctgttgc agcggcgaat
     9661 gccgccggaa atgctgggtc gggcatccag tctcaacttc ttcgtcggac tggtgatgat
     9721 gccggcctca tacgcgttag ccgttccggc ggcgcagctg atgggtgttg ccggggtgtt
     9781 cgtcatcgcg gcggtgacac ccgccgtcct ttcggtcatc gtgcatgttg cggctcggtt
     9841 gggccgagac gaaattgcgc actctctgca gtaagcagtg cgttcgcggc cagcgcaagc
     9901 gcccatcgag ttcgaggtag agccgcagca cacctcggac gatcagacgc tcagcgaccg
     9961 cctgattagg ttcattctgc tggtgatccc cgatggacgc tctcgccgag acggcttccg
    10021 aaacaccaag ttgcgcttga ctcccagatg cctaggcgac ctgctggccg aatcgcaccc
    10081 aacgcgcgag cagctgctcg gccgccccgg agtcgatggc ggcaccggcg cgctccagcc
    10141 cgtcctccca cgccggcagc cattcggcgc gactggatag gcccgcatgc gcgacgatcg
    10201 caccggcggc gttgagcacg acggcatcgc ggaccgcgcc cttggcaccg cccagcacgg
    10261 cccgcacctc ctcagcgtta gccgcagcgt caccgcccac cagttcgtcg agatcggcgc
    10321 gggcgaaccc gaatcccgca ggatcaaggg tcagcttgtc gaccgtgccc gcttgcaccc
    10381 gccagatcgt gctcgtggtg gtcgtggtca attcgtccag gccgtcgtcg ccgtgcacga
    10441 ccagcacgct ggcgcgccgc gccgcgaaca ccccggccat cacctcggcg aggttcgcga
    10501 acgcgcagcc gatcaacccg gctcgcggcc gagccgggtt cgtaagtggc ccaagcagat
    10561 tgaagaccgt cggtatcccg atctcgcggc gcaccgctga cgcgtgccgg taggacggat
    10621 ggaacaccgg cgcgaagcag aacccgatcc cgacttcggc gagactgcgc gcgacctggt
    10681 cgggtcccag atcgatgcgc acaccgaggg cctcgagcgt gtcggcgcca ccggacagcg
    10741 aggacgcggc ccggttgccg tgcttgacga ccggcacccc cgcggcggcc accacgatcg
    10801 cggccatcgt ggacaggttg acggtgttga cgccgtcgcc gccggtaccg acgacatcga
    10861 cggcgtcgtc gccgatcgcg tcaccaggga tctggcgcgc atggctcagc atcacgtcgg
    10921 cgagctcact gacctcggcc gcggtgggca ccttcatctt catcgcgacg gcgaacgcgg
    10981 cgatctgggc cgggcgcgcg ttaccggtca tgatctggtc catcgcccac gcggcctgcc
    11041 cccgcgccag gttctccccg tcggtcaaac ggcccaaaag ccgcggccac gtcggcgcga
    11101 tcccggcttt tgcgcccggg gacgtcgcgg aaaacgcccg gggcgaagga acctcagatg
    11161 acagagccac gcgccgatgg tcccacgagg accaaccgct gcccaaccgt cgtttccggt
    11221 cggtggaatg cgacgcgcgg aggcgaattt ccgccccggg tagtgttcaa caactacaaa
    11281 gcgtcatact tgcggatgtg acgagtgctg tagggacctc ggggaccgcg atcacgtcgc
    11341 gagttcattc gttgaatcgg cccaacatgg tcagtgttgg caccatagtg tggctgtcga
    11401 gcgagctcat gttctt
//
