LOCUS       NZ_BFCH01000002        10801 bp    DNA     linear   CON 22-DEC-2024
DEFINITION  Mycobacterium montefiorense strain BS sequence02, whole genome
            shotgun sequence.
ACCESSION   NZ_BFCH01000002
VERSION     NZ_BFCH01000002.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Mycobacterium montefiorense
  ORGANISM  Mycobacterium montefiorense
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium; Mycobacterium simiae complex.
REFERENCE   1
  AUTHORS   Fukano,H., Yoshida,M., Shimizu,A., Iwao,H., Katayama,Y., Omatsu,T.,
            Mizutani,T., Kurata,O., Wada,S. and Hoshino,Y.
  TITLE     Draft Genome Sequence of Mycobacterium montefiorense Isolated from
            Japanese Black Salamander (Hynobius nigrescens)
  JOURNAL   Genome Announc 6 (21), e00448-18 (2018)
   PUBMED   29798927
  REMARK    DOI:10.1128/genomeA.00448-18 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506249)
  AUTHORS   Fukano,H., Yoshida,M. and Hoshino,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2018) Contact:Yoshihiko Hoshino National Institute
            of Infectious Diseases, Mycobacteriology; 4-2-1, Higashimurayama,
            Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Platanus v. 1.1.0
            Genome Coverage                   :: 129x
            Sequencing Technology             :: Illumina Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003112775.1-RS_2024_12_22
            Annotation Date                   :: 12/22/2024 07:39:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,429
            CDSs (total)                      :: 5,376
            Genes (coding)                    :: 5,180
            CDSs (with protein)               :: 5,180
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 91 of 196
            Pseudo Genes (incomplete)         :: 88 of 196
            Pseudo Genes (internal stop)      :: 32 of 196
            Pseudo Genes (multiple problems)  :: 14 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:34:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 458257
            Orig. end                         :: 469058
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BFCH01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10801
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10801
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10801
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5801](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5801)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(31..702)
                     /locus_tag="DB708_RS03285"
                     /old_locus_tag="MmonteBS_06640"
     CDS             complement(31..702)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166359.1"
                     /locus_tag="DB708_RS03285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_06640"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_108920480.1"
                     /transl_table=11
                     /translation="MTEDGKPRFTRKGQATRTRIIDVAARLMFERGVANTSIDEVRASA
                     GVGGSQISHYFRDKRDLTREVIAARRDDVIEFHSQPRLAALDSIEALQAWADACVDDID
                     TVYRLGGCVYGSLAGELIEADNEVRDDLARGYDQWLELFHTGLIAMRQRGGLRPDADPR
                     HLAASLVIAHQGGAMITHATGDAEPLRVAVNAAVDYVRSFAVDPGTGKRGARPSAQRSP
                     R"
     gene            complement(699..1400)
                     /locus_tag="DB708_RS03290"
                     /old_locus_tag="MmonteBS_06650"
     CDS             complement(699..1400)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166358.1"
                     /locus_tag="DB708_RS03290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_06650"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_108920481.1"
                     /transl_table=11
                     /translation="MAHPDVRDEQRLTAKGRATRDRIVQAAAQLIVAEGLSASNMENVR
                     KAASVSGSQLAHYFADKGALIRAVIRSQIGVVLDFHRQPQLRELDSFDDFERWIDLNMR
                     YLRRIGTSGGTPTYHALTAQLAKSDDATRTTLAAGYWQWVDLLEAAIGRMKDNKVVTAS
                     TDPRRLALAIISAHQGGATLAFTYRAEWPHADATRFAVNYLRMFAADPDERTPRPPRRS
                     RGRRAKPRAAT"
     gene            complement(1477..2346)
                     /locus_tag="DB708_RS03295"
                     /old_locus_tag="MmonteBS_06660"
     CDS             complement(1477..2346)
                     /NRPS_PKS="Domain: Abhydrolase_1 (26-269). E-value:
                     3.5e-36. Score: 117.0. Matches aSDomain:
                     nrpspksdomains_DB708_RS03295_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262: haloalkane dehalogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379096.1"
                     /locus_tag="DB708_RS03295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_06660"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_108920482.1"
                     /sec_met_domain="PF00561 (E-value: 8.8e-28, bitscore: 92.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.5e-14, bitscore:
                     50.0, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAVHHRYATVDGHQLFFREAGDAAAPTLVLLHGFPTSSHMFRRL
                     IPELADRYHVIAPDHLGFGLSDAPAVTEFDYTFDALTDLTAGLLRSLGVDRYAMYVQDY
                     GAPIGWRLALRDPSAITAIISQNGNGYDAGFVEDFWKVVRAYQSEPTTETEAPVRQFLG
                     LDATRWQYLTGVPDETLVDPDCWHHDYALLSRPGNDLVQLKLFRDYATNAPMYPQVHEY
                     FRASRVPLLAVWGRGDEIFGPAGAEAFAADLPDAQIHLLDGGHFLLESALDEVTELIRN
                     FLKDRVPA"
     aSDomain        complement(1540..2268)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DB708_RS03295_Abhydrolase_1.1"
                     /evalue="3.50E-36"
                     /label="DB708_RS03295_Abhydrolase_1.1"
                     /locus_tag="DB708_RS03295"
                     /protein_end="269"
                     /protein_start="26"
                     /score="117.0"
                     /tool="antismash"
                     /translation="PTLVLLHGFPTSSHMFRRLIPELADRYHVIAPDHLGFGLSDAPAV
                     TEFDYTFDALTDLTAGLLRSLGVDRYAMYVQDYGAPIGWRLALRDPSAITAIISQNGNG
                     YDAGFVEDFWKVVRAYQSEPTTETEAPVRQFLGLDATRWQYLTGVPDETLVDPDCWHHD
                     YALLSRPGNDLVQLKLFRDYATNAPMYPQVHEYFRASRVPLLAVWGRGDEIFGPAGAEA
                     FAADLPDAQIHLLDGGHFLLE"
     gene            2538..3245
                     /locus_tag="DB708_RS03300"
                     /old_locus_tag="MmonteBS_06670"
     CDS             2538..3245
                     /NRPS_PKS="Domain: PKS_KR (5-155). E-value: 1.3e-09. Score:
                     30.1. Matches aSDomain:
                     nrpspksdomains_DB708_RS03300_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772629.1"
                     /locus_tag="DB708_RS03300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_06670"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_165839717.1"
                     /transl_table=11
                     /translation="MLQDKTVVVVGRGSGIARAVALLARSEGARVVVAGRDEGKLATAY
                     RDTDVTAEVVDLTDDASIAALADRLGAVDHVVSTASARARGNLADLERGNLALSFGTKV
                     IGPTMLAKYVAPHINPGGSFVLFSGVHAFKLNAGYLGVGITNGAVDFLTRWLAVELAPT
                     RVNAISPGVIDTGAWDSLGEDGKRDYFQHIAAGNPVRRIGTSQDVANAVLFAMTNTFMT
                     GMTLKIDGGEPLI"
     aSDomain        2553..3002
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DB708_RS03300_PKS_KR.1"
                     /evalue="1.30E-09"
                     /label="DB708_RS03300_PKS_KR.1"
                     /locus_tag="DB708_RS03300"
                     /protein_end="155"
                     /protein_start="5"
                     /score="30.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TVVVVGRGSGIARAVALLARSEGARVVVAGRDEGKLATAYRDTDV
                     TAEVVDLTDDASIAALADRLGAVDHVVSTASARARGNLADLERGNLALSFGTKVIGPTM
                     LAKYVAPHINPGGSFVLFSGVHAFKLNAGYLGVGITNGAVDFLTRW"
     gene            3274..4968
                     /locus_tag="DB708_RS03305"
                     /old_locus_tag="MmonteBS_06680"
     CDS             3274..4968
                     /EC_number="2.8.1.9"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1094: ferredoxin"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951923.1"
                     /locus_tag="DB708_RS03305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_06680"
                     /product="MOSC and FAD-binding oxidoreductase
                     domain-containing protein"
                     /protein_id="WP_108920484.1"
                     /transl_table=11
                     /translation="MGRLVSVNMGMPKNVRWRDKTVYTGIWKTPVEGPIMVRRLNIDGD
                     GQGDLGGHGGEQRAVMVYQTESYDFWKAYLNRDDLQPGHFGENFTIAGLPDHDVCIGDR
                     YRIGNAEFEVTQPRVTCFRVGVRLDEPEMPNLLVSQRRPGFYFRVITEGCVQAGDDIVR
                     TRRGRHELSVADVDALLYLPDRNAEQLRKVVDVPALSPGWQQSFRDMLASQDGPAPTVG
                     FEPGWNGFRPLRVSEIRRENPQVMSIGLQAEDGAALPPALPGQYLTVRIPGAGDPVPLR
                     SYSLSSAASDYRISVKREDHGLVSRWLHAHIEPGSIVEAAAPRGDFYLIDDERPVVMIS
                     AGIGVTPVLAMLHALAATRSSRDIWWLHTTRDRETQAFAAEVTTLVESLPQARELVFYT
                     QTQGRLGQPAIAALGLPTDAAAYLCGPTQFMTDMREALVAAGLDGAHIHSELFGALPPI
                     NPGIVDGAARKPPHVPPGPPGTGPSITFARSGLTVQWSSDYGSILDFAEACDVPTRFSC
                     RSGVCHVCETGVVAGVTSYVQPPLEAPGPGSVLICSAAPDTDLVLDL"
     gene            complement(5001..5801)
                     /locus_tag="DB708_RS03310"
                     /old_locus_tag="MmonteBS_06690"
     CDS             complement(5001..5801)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263893.1"
                     /locus_tag="DB708_RS03310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_06690"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_108920485.1"
                     /sec_met_domain="Linocin_M18 (E-value: 5.9e-93, bitscore:
                     305.5, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNNLYRDLAPITDLAWAEIELEATRTFKRHIAGRRVVDVSEPGG
                     PVTAGVSTGRLVDVQAPTDGVEAHLRTSKPLVRLRVPFTLSRDEIDDVERGSQDSDWDP
                     VKAAAKKLAFVEDRAIFEGYPAAEIDGIRNCSSNPALMLPDDPRAIPDIITQALSELRL
                     AGVDGPYSVLLSADIYTRVSETTEHGYPILEHINRLVNGDIIWAPAIDGAFVLTTRGGD
                     FDLQLGTDVSIGYTSHDADSVQLYLQETLTFLCYTAEASVALGA"
     gene            complement(5798..6859)
                     /locus_tag="DB708_RS03315"
                     /old_locus_tag="MmonteBS_06700"
     CDS             complement(5798..6859)
                     /EC_number="1.11.1.-"
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877197.1"
                     /locus_tag="DB708_RS03315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_06700"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_201263407.1"
                     /transl_table=11
                     /translation="MAVRDYTVNDRVAHVPPVQPQPILAPLTPAAIFLVVTIDDGGEAP
                     VHDALADISGLVRAIGFRDPTKHLSVITSIGSEAWDRLFGGPRPAELHPFIELSGPRHL
                     APATAGDLLFHVRAETMDVCFELAGRILKSMAGAVTVVDEVHGFKFFDNRDLLGFVDGT
                     ENPDGPIAVSATAIGDEDPDFAGSCYVHIQKYVHDMSSWDSLSVTEQERVIGRTKLEDI
                     ELDDDVKPADSHLALNVIEDDDGNELKIVRANMPFGELGAGEFGTYYIGYSRTPAVTER
                     MLRNMFIGDPPGNTDRILDFSTAVTGGLFFSPTVDFLDDPPPLPAPLETERPDPPTAHD
                     GALSIGSLKGTTQ"
     gene            6870..8126
                     /locus_tag="DB708_RS03320"
                     /old_locus_tag="MmonteBS_06710"
     CDS             6870..8126
                     /GO_function="GO:0004177 - aminopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215315.1"
                     /locus_tag="DB708_RS03320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_06710"
                     /product="M18 family aminopeptidase"
                     /protein_id="WP_165839710.1"
                     /transl_table=11
                     /translation="MSASAAGLCEFIDASPSPFHACATVAGRLGAAGYAELREADPWPG
                     QPGRYFTVRAGSLVAWNTTGPDGPFRIVGAHTDSPNLRVKQHPDRVVAGWQVVALEPYG
                     GAWLNSWLDRDLGISGRLSVRAGSGLDHRLIRIDEPILRIPQLAIHLAEDRKSLTLDPQ
                     RHVNAVWGAGPQARAFVDYVAERAGVAPADVLAADLMTHDLTPSTVIGADASLLSAPRL
                     DNQASCYAGLEALLAAEPRGNVAVLVMFDHEEVGSSSDHGAQSNLLSTVLERIVLAAGG
                     GRGDFLRRLPTSLLASADMAHATHPNYPERHEPGHLIEINAGPVLKVHPNLRYATDGRT
                     AAAFALACDQAGVALQRYEHRADLPCGSTIGPLASAQTGIPTVDVGAAQLAMHSARELM
                     GAHDVAAYSAAMRAFFSPE"
     gene            complement(8130..8717)
                     /locus_tag="DB708_RS03325"
                     /old_locus_tag="MmonteBS_06720"
     CDS             complement(8130..8717)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1224: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212205.1"
                     /locus_tag="DB708_RS03325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_06720"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_108920581.1"
                     /transl_table=11
                     /translation="MPNERVGRPRDARLNHAILAATRELLTTGSYAELTMESVASRARV
                     GKKTLYRRWSSKAPLVAEAVLEAYGGSGSFPVAETADVRADLQRWLREHADFLAGPSHT
                     ALVRALIAAAAARPADGEDLYQQLSAPQMDGLMTRLRSAVEAGQLRADADLEAVAEALI
                     GTLLFRALTGPGAGGRFDGLLDAILGGVARPG"
     gene            8802..9872
                     /locus_tag="DB708_RS03330"
                     /old_locus_tag="MmonteBS_06730"
     CDS             8802..9872
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244915.1"
                     /locus_tag="DB708_RS03330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MmonteBS_06730"
                     /product="phosphotransferase"
                     /protein_id="WP_108920487.1"
                     /sec_met_domain="APH (E-value: 2.7e-10, bitscore: 35.4,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSATSLSVPKTWDDITPEWMSAALAPDYPGAEVDTVSVEHRDDGT
                     NRRARLGVTYAAGAGPATVFVKAADPAHKALIRLTSGMFHEPRLFTCGVDLPLEHPAVY
                     TALIDEADYDFVLVMEDLRARGADPRDGTRPMTVDEVATGVRGLGRMHGQYWGERVLCA
                     PALGWLEPFLPWDGMQAAPLPTALERLGDDAPTEVTSLTIDTLIDSIWKPYIASLTTSP
                     QTLLHGDPHIGNTYLLPGGEVGFLDWQVARRGNWSLDLGYFLQGALTVEDRRRSERDLL
                     TEYRDSLGLAADELPSADEIWLRYRASVAHGLTLWLVTASAGEWQRTDVSVALAQRYSF
                     AYADLDAASALAEIVA"
     misc_feature    complement(9976..9990)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 ccactgctct aagcggggcg tgccgcggcg tcatcgaggc gatcgttgcg cagacggcct
       61 ggcgcccctt ttaccggtcc cgggatcgac cgcgaacgaa cgcacgtagt cgaccgcggc
      121 gttgacggcg acccgtaagg gctcggcgtc acccgtggcg tgcgtgatca tcgcaccacc
      181 ctgatgcgcg atgaccagcg aggccgccaa gtgccgcggg tcggcgtcgg ggcgcagacc
      241 gccgcgctgg cgcatcgcga taaggccggt gtggaacagc tccagccatt ggtcgtagcc
      301 gcgcgcgagg tcgtcgcgaa cttcgttgtc ggcttcgatc agttcgcccg ccagtgagcc
      361 gtagacgcag ccgcccagcc gatacacggt gtcgatgtcg tcgacgcaag cgtcggccca
      421 ggcttgcagc gcctcgatgc tgtcgagcgc agcgagccgc ggttggctgt gaaactcgat
      481 gacgtcgtcg cggcgcgcgg cgatgacctc gcgagtcagg tcgcgtttgt cgcggaagta
      541 gtgcgagatc tgcgatccgc ccaccccggc gctagcacga acctcgtcga tgctggtgtt
      601 ggccacgcca cgctcgaaca tcagccgggc cgcgacatcg atgatgcgag tgcgcgtcgc
      661 ctgtcctttg cgggtgaagc gcggcttgcc gtcttcagtc atgttgccgc ccttggcttc
      721 gcgcgcctac cccgagaacg ccgtggcggc cgtggcgtgc gttcatcggg atcggcggca
      781 aacattcgca ggtagttgac cgcaaaccgg gtggcgtcgg cgtgcggcca ttccgcccga
      841 taggtgaacg ccaacgtggc gccgccttga tgagcgctga tgatcgccag cgccagccgt
      901 cgtggatcgg tgctcgccgt cacgactttg ttgtccttca tccgcccgat ggccgcctcg
      961 agcaagtcca cccactgcca gtagccggcc gccagggtgg tgcgggtagc gtcgtcggac
     1021 ttggccaact gcgcggtcag cgcgtggtaa gtgggtgtgc caccggaggt tccgatgcgc
     1081 cggaggtagc gcatgttgag gtcgatccat cgctcgaagt cgtcgaacga gtccagctcc
     1141 cgtagctggg gctggcggtg gaagtccaga acgaccccga tctgactcct gatcactgcg
     1201 cgaatcaggg cgcccttgtc ggcgaaataa tgggcgagct gagagccgct gaccgacgcg
     1261 gccttacgca cgttctccat attcgaggcc gataggcctt cggcgacgat cagttgggcg
     1321 gcggcctgca caatcctgtc gcgggtggca cgccccttgg cggtcaaccg ttgttcgtcg
     1381 cgaacatcgg gatgggccat cgcgtcaggc taacccgcga gcaccgccga ttatgggatc
     1441 tatggcccat tgctggctgc gccgcccggg ccccggtcag gcgggcacgc ggtccttgag
     1501 gaagttgcgg atcagctcgg tgacctcatc gagtgcggac tccaacagga aatgtccgcc
     1561 gtcgagcaga tggatctgcg catcgggcag gtcggcggcg aaggcctcgg caccggcggg
     1621 cccgaagatc tcgtcaccgc gtccccatac cgcgagcagc gggaccctgc tggcgcgaaa
     1681 gtactcgtgc acttgggggt acatcggcgc gttggtggcg tagtcgcgga atagcttcag
     1741 ttggaccagg tcattaccgg gccgggacag caacgcgtag tcgtgatgcc agcagtcggg
     1801 gtcgaccaac gtctcgtcgg gtacgccggt gaggtattgc cagcgggtcg cgtccaggcc
     1861 gagaaattgg cgcaccggag cctcggtctc ggtggtcggc tcagactggt aggcccgcac
     1921 gaccttccag aaatcctcca cgaagccggc gtcgtagccg ttgccgttct ggctgatgat
     1981 cgccgtaatg gccgacgggt cgcgcagggc caaccgccat ccgatcgggg cgccgtaatc
     2041 ctgcacgtac atcgcgtacc gatcgactcc caggctgcgc agcaggcccg cggtcaggtc
     2101 ggtcagcgca tcgaaggtgt agtcgaactc cgtgacagcc ggagcatcgg agagtccgaa
     2161 gcccaggtga tccggcgcga tcacgtgata gcggtcggcg agctccggaa tgagacgccg
     2221 gaacatgtgc gagctggtcg gaaatccgtg cagcaacacc aacgtggggg ccgccgcgtc
     2281 gccggcttcc cggaagaaca gctgatggcc gtcgacggtg gcgtaacgat gatggacagc
     2341 ggacatgaga ttcctcctgc gccaggtgag tctggtcgtc ggccgactca ctcgcacttt
     2401 tgggatatac atcccagtat gcatcaaatc caccggctcg caatagcccc cgctggagtt
     2461 gaccgctcca ttagccggga atattctggg tcaactatcc caattctgtg ccacgccgag
     2521 gagcattcgt gggtaaaatg ttgcaggaca agaccgtcgt ggtggtagga cgcggcagcg
     2581 ggatcgcgcg agcggtcgct ttgttggcgc ggtcggaagg tgcgcgcgtc gtcgtggcag
     2641 ggcgcgacga gggcaaactt gcaaccgcct accgcgacac cgacgtcacc gccgaggtgg
     2701 tcgacctcac cgacgacgcg tcgattgccg ctctggccga tcggttgggt gcggtggatc
     2761 acgtggtatc gaccgcgtcg gcgcgtgccc gggggaacct ggccgatctt gagcgcggaa
     2821 accttgcgct atcgttcggc accaaagtca tcggtccgac gatgctagcc aaatatgttg
     2881 caccgcatat taacccgggc ggatcgttcg ttctattctc gggcgttcat gccttcaagc
     2941 tgaacgccgg ctacctcgga gtgggtatca ccaatggagc cgtggacttc cttacccgtt
     3001 ggctcgcggt cgaactcgca ccgacgcggg tcaacgcgat ctcgccggga gtcatcgata
     3061 ccggcgcctg ggattcgctg ggcgaggacg gcaaacgtga ttatttccag cacatcgctg
     3121 ccggaaaccc ggtgcggcgg atcgggacct cgcaggatgt ggccaacgcg gtgctgttcg
     3181 ccatgaccaa cacgttcatg accggtatga cgttgaagat cgacggcggc gagccgctga
     3241 tttaggcttc tggagcggcc aaaaggagaa gtgatgggca ggcttgtttc ggtgaatatg
     3301 ggcatgccca aaaacgtgcg atggcgcgac aagacggtct ataccgggat ctggaagacg
     3361 ccggtcgaag gcccgatcat ggtgcgacgt ctcaacatcg acggtgacgg tcaaggcgat
     3421 ctcggcggcc acggtggcga acaacgcgcg gtcatggttt accagacgga gtcctacgac
     3481 ttctggaagg cttatctgaa ccgcgacgac ctgcaacccg gccacttcgg cgaaaacttc
     3541 accatcgccg ggttgcccga tcacgatgtg tgcatcggcg atcgttaccg catcgggaac
     3601 gccgaattcg aggtgaccca gccgcgggtc acctgctttc gggtcggcgt gcggctggac
     3661 gaaccggaaa tgccgaacct gcttgtgtcg caacgacgtc caggcttcta tttccgcgtc
     3721 atcaccgagg ggtgcgtcca ggccggcgac gacatcgtgc ggacccggcg tggccgtcac
     3781 gaactcagtg tcgccgacgt cgacgcgctg ctgtacctgc cggaccgcaa tgccgaacaa
     3841 ctccgcaagg tcgtcgacgt tccggcgctg agcccaggct ggcagcagtc attccgcgac
     3901 atgctcgcct cgcaagacgg tccggcgccg accgtcgggt tcgagccggg gtggaacggc
     3961 tttcgcccgt tgcgggtcag cgagatacgc cgggagaacc cacaggtgat gtctattggt
     4021 ctgcaggccg aagacggggc cgcgctgcca ccggcactgc cgggccaata cctcacggtg
     4081 cggatcccgg gcgccggcga tccggtgccg ctgcgcagtt actccctatc ctcggcggcg
     4141 agcgattacc gcatcagcgt caaacgcgaa gaccacggac tggtgagccg gtggctgcac
     4201 gcgcacatcg aacccggttc gatcgtcgaa gccgcggctc cgcgtggcga tttctacctc
     4261 atcgatgacg aacggccggt ggtcatgatc tcggcgggca tcggtgtgac tccggttttg
     4321 gcgatgttgc atgcgctggc cgcgacccgc agtagccgcg acatatggtg gctgcacacc
     4381 acccgcgatc gcgaaaccca ggcttttgcg gccgaagtca cgactctggt cgagtcgttg
     4441 ccccaggctc gagagctcgt gttctacacc cagacccaag ggcgcttggg acagccggcc
     4501 atcgcggcac tcggactgcc gactgatgcc gcggcatacc tttgcggccc aacccaattc
     4561 atgacagaca tgcgcgaggc gctggtggcc gccgggctcg acggtgcgca catccacagc
     4621 gaactcttcg gtgcgctgcc accgataaac cccggcatcg tcgatggcgc ggcgcgcaag
     4681 ccaccacacg tgccgcccgg cccgccgggg accggcccgt cgatcacctt cgcccgcagc
     4741 gggcttaccg tccagtggtc ctcggactac ggcagcatcc tggatttcgc ggaagcctgc
     4801 gacgtgccga cccgtttctc ctgccgaagc ggtgtctgcc acgtgtgcga gaccggagtg
     4861 gtcgcgggtg tgaccagcta cgtgcagccg ccattggagg cgccgggccc gggcagcgtg
     4921 ctgatctgct cggcggcgcc ggacaccgac ctcgtgctgg atctgtgaat aagcgaaagc
     4981 cgttgcgcta gcccgatttc ctaagcgccc agcgcgaccg atgcctcggc ggtgtagcac
     5041 aggaacgtca gggtctcctg cagatatagc tgcactgagt cggcgtcatg gctggtgtaa
     5101 ccaatggaga cgtcggtgcc cagttgcaga tcgaagtcac cgccacgcgt ggtgagcacg
     5161 aacgcaccgt cgatggcggg cgcccagatg atgtcgccgt tcaccagccg gttgatgtgt
     5221 tcgaggatcg gatacccgtg ctcggtcgtc tcgctgaccc tcgtgtagat atcggcagac
     5281 agcagcaccg aataagggcc gtcgacaccg gccagccgca gctcggacag cgcctgcgtg
     5341 atgatgtcgg ggatcgcgcg aggatcgtcg ggcaacatta gtgcggggtt cgagctgcaa
     5401 ttgcggatcc cgtcgatctc cgcggcaggg tagccctcga agatggcgcg gtcttcgacg
     5461 aaggccagct tcttggcggc ggccttgacc ggatcccagt cggagtcctg cgagccgcgt
     5521 tcgacgtcgt ctatctcgtc gcgcgacagg gtaaatggaa cccgcaggcg gacaagcggt
     5581 ttgctggtgc gcaggtgcgc ttctacgccg tcggtgggtg cttgcacgtc taccaggcgc
     5641 cccgtgctga cccccgcggt gaccggaccg ccgggctcgc tgacgtcgac cacccggcgc
     5701 ccggcgatgt gtcgcttgaa cgtccgcgtc gcctccaatt cgatttcggc ccaagcgagg
     5761 tcggtgatcg gcgctagatc gcggtagagg ttgttcttca ttgagtggtt cctttcagac
     5821 tgccgatcga aagtgcgccg tcgtgtgcgg tgggcgggtc cggtcgctcc gtctcgagtg
     5881 gcgcggggag gggtggtgga tcgtcgagga agtcgacggt gggggagaag aacagtccgc
     5941 ccgtgacggc ggtggagaaa tccaggatac ggtcggtgtt gccgggcgga tcgccgatga
     6001 acatattgcg cagcatccgt tcggtgactg ccggtgtgcg cgaatagccg atgtagtacg
     6061 tgccgaattc gccggcgccg agttcgccga acggcatgtt ggcccgcacg atcttcagct
     6121 cgttgccgtc gtcgtcttcg atgacgttga gcgcgaggtg ggagtcggcc ggcttcacgt
     6181 cgtcgtcgag ttcgatgtct tccagtttgg tccggccgat cacccgttcc tgctcggtaa
     6241 ccgacagcga atcccacgac gacatgtcgt gtacgtattt ctggatgtgt acgtagcacg
     6301 atccggcgaa gtcgggatcc tcgtcgccga ttgcggtggc gctgacggcg attgggccat
     6361 cggggttttc ggtgccgtcg acaaagccga gcaggtctcg gttgtcgaag aacttgaacc
     6421 cgtgcacttc gtcgacgaca gtgaccgcgc cggccatcga cttgaggatg cgaccggcca
     6481 gttcgaagca cacgtccatc gtctcggccc ggacgtggaa caacaggtcg ccggcggtgg
     6541 ccggtgctaa gtgccgcgga ccgctcagct cgatgaacgg atgcagttcg gcgggtcgcg
     6601 ggccgccgaa caatcgatcc caagcttccg agccgatcga ggtgatcacc gaaaggtgct
     6661 tggtcgggtc acgaaagccg atggcgcgca ccagtcccga gatgtcggcc agcgcgtcat
     6721 gtaccggcgc ctcgccgccg tcgtcgatgg tgaccaccaa gaaaatcgcg gcgggcgtca
     6781 acggcgccag gatgggctgg ggctgaaccg gaggcacatg ggcgacccta tcgttaactg
     6841 tgtaatcgcg gacagccatg atgttgaaca tgtcggccag cgccgcaggc ctctgcgaat
     6901 tcatcgacgc ctccccgtcc ccgtttcatg cctgcgccac ggtcgccggc cgactgggcg
     6961 ccgccggcta cgcggaactc cgggaagccg acccctggcc cgggcaaccg gggcgctact
     7021 tcaccgtccg cgccggctca ctggtcgcct ggaacacaac cggtcccgac ggtccattcc
     7081 ggatcgttgg cgcgcacacc gacagtccca acctgcgggt caagcagcat ccggaccggg
     7141 tcgtcgccgg ctggcaggtg gtggcgctgg agccctacgg cggggcgtgg ctcaactcct
     7201 ggctcgatcg cgacctgggg atcagcggcc ggttgtcggt gcgcgccggc tcagggctgg
     7261 atcatcggct gatccgaatc gacgagccga ttctgcggat cccgcagctg gccatccatc
     7321 tggccgagga tcgcaagtcg ctgaccctgg atccgcagcg gcacgtcaat gcggtctggg
     7381 gcgctggacc ccaagcgcgg gcgttcgtgg actacgtcgc cgagcgggcc ggggtggctc
     7441 ctgccgacgt gctggccgcc gacctgatga cccacgacct gacgccgtcg acggtgatcg
     7501 gcgcggacgc cagcctgctc agcgcacccc ggctggataa ccaggccagt tgctatgcgg
     7561 gcctggaggc gttgcttgcg gccgagccgc gcggcaacgt ggcggtgctg gtgatgttcg
     7621 accacgagga ggtcgggtcg tcgtcggacc acggcgcgca gtccaacctg ctgagcacgg
     7681 tgctggaacg catcgtgctc gcggccggcg gtggccgggg agacttcctg cggcggctgc
     7741 ccacgtcgct actggcctcg gccgacatgg cgcatgccac ccaccccaac tacccggagc
     7801 gccacgagcc cggccacttg atcgagatca acgcggggcc ggtgctcaag gtgcacccga
     7861 atctgcggta cgcgaccgac gggcgtacgg cggcggcatt cgcgctggcc tgcgatcagg
     7921 ccggggtcgc gctgcagcgc tacgagcatc gcgccgacct gccgtgcggt tccacgatcg
     7981 ggccgctggc ctcggcgcaa accggtattc ccaccgtcga cgtcggtgcc gcgcagctcg
     8041 ccatgcactc cgcacgcgaa ttgatgggcg cacacgacgt ggccgcctac tcggcggcga
     8101 tgcgtgcgtt cttctccccg gagtgatcct cacccgggcc tcgccacgcc cccgagtatc
     8161 gcgtcgagca gcccatcgaa acgcccgccg gcgcccgggc cggtaagcgc gcgaaacagc
     8221 aaggtgccga tcaacgcctc ggcgacggct tcgaggtcgg catcggcgcg cagctggccg
     8281 gcctcgacgg cggaccgtag acgcgtcatc agcccgtcca tctggggcgc actcagctgc
     8341 tggtagagat cctcgccatc ggccgggcgg gcggcggccg cggcgatcag ggctcgaacc
     8401 aaagcggtgt gcgacggccc ggccaagaaa tcggcatgct cgcggagcca acgctgcagg
     8461 tcggcgcgca cgtctgcggt ctcggcgacg ggaaacgatc ccgatccgcc gtaggcctcc
     8521 agcacggctt cggcgaccaa cggcgccttc gatgaccagc gccggtagag ggttttcttc
     8581 ccgacgcgtg cgcggctggc aacgctttcc atcgtcagct cggcgtaact gccggtagtg
     8641 agaagttcgc gggtggccgc gaggatcgcg tgattcaacc gtgcgtcccg cggacgaccg
     8701 accctttcgt tgggcactgg cggttggggg cgcggttggg ctaacttcat ggtgcacagt
     8761 ttaggaaacg atacgtatcg tttcttcgaa aggactcgcc ggtgagtgcc acgtcgttgt
     8821 cggtgcccaa gacctgggac gacatcacgc ccgaatggat gtcggccgca ctggccccgg
     8881 actatcccgg cgccgaagtc gacacggtct ccgtcgaaca tcgtgacgac ggcactaacc
     8941 gacgtgcccg gttgggcgtc acctatgccg ccggtgcggg tccggcgacg gttttcgtca
     9001 aagccgccga tcccgcgcac aaagctctga tccgcttgac cagtggcatg ttccacgagc
     9061 cgcggctttt cacctgcggg gtcgatttgc cgctcgagca tcccgccgtc tacaccgcgc
     9121 tgatcgatga ggccgactac gacttcgtcc tggtgatgga agatctgcga gcccggggag
     9181 ccgatccccg cgacggcacc cggccgatga ccgtcgatga ggttgccacg ggcgtgcgtg
     9241 gcctgggccg aatgcacggt cagtattggg gtgaacgcgt gctgtgtgcc ccggcgctcg
     9301 gatggctcga acccttcctg ccctgggacg gcatgcaagc ggcaccgttg cccaccgccc
     9361 tcgagcgcct gggcgatgac gctcccaccg aggtgacgtc cttgaccatc gacaccctga
     9421 tcgactcgat ctggaagccc tacatcgcgt cgctgaccac gtcgccgcag accttgctgc
     9481 acggcgaccc ccacatcggg aacacctacc tgctacccgg cggcgaggtg ggattcctgg
     9541 attggcaggt ggcacgccgg ggcaattggt cgctggatct gggctatttc ctgcagggcg
     9601 ccctgacggt tgaggatcgc cgtcgcagcg aacgcgacct gctcaccgag taccgggact
     9661 cgctgggact agcggccgac gagttgccca gtgccgatga gatctggttg cgctaccggg
     9721 cctcggtggc ccacggactg accctgtggc tggtgaccgc tagcgccggt gagtggcaac
     9781 gcacggacgt ctcggtcgct ctggcacaac ggtattcgtt cgcctacgcc gatctcgatg
     9841 cggcgtcggc gctggccgag atcgtcgcgt gagtgtgctg tgcggctgac gctcaaagcg
     9901 cccgactgct gcgcggcgag tagctcttta gactgttctg cgtgactgtc tccggagcgg
     9961 gcgcccgtac tcacgtggcc gacaccgtcg aacacgcagc cgccagcccc gaccaaccgc
    10021 agccgttcgg ggaattgggc cttaaagacg acgagtacca gcggattcgc gagattctcg
    10081 gccgcaggcc caccgacacc gagctggcga tgtactcggt gatgtggagc gagcactgct
    10141 cgtacaagtc gtcaaaggtt catctgcgct acttcggcga gaccaccacc gacgcgatgc
    10201 gcgcggccat gctggccggc atcggcgaga acgcgggtgt cgtggacatc ggtgacggtt
    10261 gggccgtcac gttcaaggtc gagtcgcaca accacccgtc ctacgtcgag ccctaccagg
    10321 gcgcggccac cggcgtgggc ggcatcgtgc gcgacatcat ggccatgggc gcacgaccgg
    10381 tcgccgtgat ggatcagctc cggttcggcg ccgccgacgc gccggacacc cgccgggtgc
    10441 tcgacggcgt ggtccgcggg atcggcggct acggcaactc gctggggctg cccaacatcg
    10501 gtggcgaaac cgtcttcgac gcgtcgtacg cgggcaaccc gttggtcaac gcgctatgtg
    10561 tgggcgtgct gcgcaaggag gatctacacc tggccttcgc ctcgggaacc ggcaacaaga
    10621 tcatcctgtt cggcgcgcgc accggcctcg acggtatcgg tggagtgtcc gtgctggcgt
    10681 cggagacgtt cggtggcgac gagggcggcg gtcccggccg caagaagctg ccctcggtgc
    10741 aggtcggcga cccgttcacc gagaaggtgc tcatcgaatg ctgcctcgag ttgtacgcag
    10801 c
//
