GCF_003072605.2 - 9 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP026736.1 (Priestia megaterium (Bacillus megaterium))

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	terpene	162,065	182,883
Region 1.2	NI-siderophore	344,336	378,911	Low	schizokinen	NRPS:Type IV
Region 1.3	terpene-precursor	751,830	772,726
Region 1.4	terpene	2,205,728	2,226,576	Low	carotenoid	terpene
Region 1.5	T3PKS	3,003,121	3,044,206
Region 1.6	RRE-containing	3,309,352	3,330,458
Region 1.7	terpene	3,703,105	3,724,982
Region 1.8	lassopeptide	3,752,581	3,781,332	High	paeninodin	ribosomal:unmodified

NZ_CP026738.1 (Priestia megaterium (Bacillus megaterium))

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 3.1	T2PKS	1	67,944

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	terpene	162,065	182,883
Region 1.2	NI-siderophore	344,336	378,911	Low	schizokinen	NRPS:Type IV
Region 1.3	terpene-precursor	751,830	772,726
Region 1.4	terpene	2,205,728	2,226,576	Low	carotenoid	terpene
Region 1.5	T3PKS	3,003,121	3,044,206
Region 1.6	RRE-containing	3,309,352	3,330,458
Region 1.7	terpene	3,703,105	3,724,982
Region 1.8	lassopeptide	3,752,581	3,781,332	High	paeninodin	ribosomal:unmodified
Region 3.1	T2PKS	1	67,944

No secondary metabolite regions were found in these records:: NZ_CP026737.1 (Priestia megaterium (Bacillus megaterium)); NZ_CP026739.1 (Priestia megaterium (Bacillus megaterium)); NZ_CP026740.1 (Priestia megaterium (Bacillus megaterium))

Compact view

NZ_CP026736.1 - Region 1 - terpene

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Location: 162,065 - 182,883 nt. (total: 20,819 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

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MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

C2I28_RS01020: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Lyases: hydratase

C2I28_RS01010: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002084.4	0.56	ribosomal	biarylitide YYH	Planomonospora sp. ID82291
BGC0002099.3	0.56	ribosomal	biarylitide YFH	Planomonospora sp. ID107089
BGC0002631.2	0.53	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0001201.3	0.52	terpene	eremophilene	Sorangium cellulosum So ce56
BGC0002103.2	0.45	other (other)	pulcherriminic acid	Bacillus subtilis subsp. subtilis str. 168
BGC0000902.4	0.45	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0000285.5	0.43	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0001336.5	0.42	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0001485.4	0.42	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0000537.3	0.41	ribosomal	nisin U	Streptococcus uberis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002084.4	0.56	ribosomal	biarylitide YYH	Planomonospora sp. ID82291
BGC0002099.3	0.56	ribosomal	biarylitide YFH	Planomonospora sp. ID107089
BGC0002631.2	0.53	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0001201.3	0.52	terpene	eremophilene	Sorangium cellulosum So ce56
BGC0002103.2	0.45	other (other)	pulcherriminic acid	Bacillus subtilis subsp. subtilis str. 168
BGC0000902.4	0.45	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0000285.5	0.43	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0001336.5	0.42	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0001485.4	0.42	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0000537.3	0.41	ribosomal	nisin U	Streptococcus uberis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene		squalene	C30	acyclic	none
triterpene	C30 carotenoid	diapophytoene	C30	acyclic	none
triterpene		PSPP	C30	acyclic	PP
tetraterpene	C40 carotenoid	phytoene	C40	acyclic	none
triterpene		hydroxysqualene	C30	acyclic	OH

Genes with terpene domains:

C2I28_RS00975: PT_phytoene_like

Domain 1 (15 - 208): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → squalene; FPP → diapophytoene; FPP → PSPP; GGPP → phytoene; PSPP → hydroxysqualene

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.34 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.70 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.25 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.87 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 24.37 out of 27.78

Confidence: strong

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.95 out of 28.19

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 16.22 out of 18.16

Confidence: strong

Additional weak hits (2):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 22.43 out of 28.14

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 27.39 out of 36.54

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PP	Diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MogR	Repressor of flagellar motility genes
ZuR variant 1	Zinc-responsive repressor

NZ_CP026736.1 - Region 2 - NI-siderophore

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Location: 344,336 - 378,911 nt. (total: 34,576 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

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MIBiG comparison

KnownClusterBlast

SubClusterBlast

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

C2I28_RS01930: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

C2I28_RS01990: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Transferases: aminotransferase formyltransferase

C2I28_RS01915: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

C2I28_RS01950: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

C2I28_RS02000: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Lyases: decarboxylase

C2I28_RS01945: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Ligases: synthase

C2I28_RS01895: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002683.2	0.67	NRPS	schizokinen	Nostoc sp. PCC 7120 = FACHB-418
BGC0002633.2	0.65	other (other)	schizokinen	Leptolyngbya sp. PCC 7376
BGC0001478.5	0.61	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.60	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001572.3	0.59	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002692.2	0.59	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0002469.2	0.59	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0001531.5	0.59	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002305.2	0.57	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0002470.2	0.57	other (other)	synechobactin C9, synechobactin C11, synechobactin 13, synechobactin 14, synechobactin 16, synechobactin A, synechobactin B, synechobactin C	Synechococcus sp. PCC 7002

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002683.2	0.67	NRPS	schizokinen	Nostoc sp. PCC 7120 = FACHB-418
BGC0002633.2	0.65	other (other)	schizokinen	Leptolyngbya sp. PCC 7376
BGC0001478.5	0.61	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.60	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001572.3	0.59	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002692.2	0.59	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0002469.2	0.59	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0001531.5	0.59	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002305.2	0.57	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0002470.2	0.57	other (other)	synechobactin C9, synechobactin C11, synechobactin 13, synechobactin 14, synechobactin 16, synechobactin A, synechobactin B, synechobactin C	Synechococcus sp. PCC 7002

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 22.86 out of 26.12

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.36 out of 26.06

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 28.43 out of 29.38

Confidence: strong

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 27.77 out of 29.38

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 18.25 out of 18.46

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 24.08 out of 27.78

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.00 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.33 out of 26.06

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.91 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 26.32 out of 29.38

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.42 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.52 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.70 out of 26.06

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 24.73 out of 28.19

Confidence: strong

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.98 out of 28.19

Confidence: medium

Additional weak hits (6):

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.99 out of 20.01

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.27 out of 25.88

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.14 out of 32.74

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 24.04 out of 28.14

Confidence: weak

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 16.74 out of 20.47

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.54 out of 30.22

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
FuR variant 1	Ferric Uptake Regulator
LuxO	Repressor of luminescence
MogR	Repressor of flagellar motility genes
RpoN	Regulator of nitrogen assimilation and virulence
ZuR variant 1	Zinc-responsive repressor

NZ_CP026736.1 - Region 3 - terpene-precursor

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Location: 751,830 - 772,726 nt. (total: 20,897 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: carboxylase

C2I28_RS03935: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

Ligases: synthase synthetase

C2I28_RS03905: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

C2I28_RS03910: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002284.3	0.40	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0001594.3	0.38	other (other)	fischerindole L	Fischerella muscicola UTEX 1829
BGC0000665.5	0.38	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001075.5	0.38	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0000642.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC413

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002284.3	0.40	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0001594.3	0.38	other (other)	fischerindole L	Fischerella muscicola UTEX 1829
BGC0000665.5	0.38	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001075.5	0.38	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0000642.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC413

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

C2I28_RS03910: PT_FPPS_like

Domain 1 (35 - 297): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: IPP, DMAPP → FPP

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.45 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.15 out of 26.06

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 21.16 out of 25.88

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 17.14 out of 18.16

Confidence: strong

Additional weak hits (4):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.29 out of 25.75

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 27.09 out of 39.62

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 23.34 out of 31.73

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.65 out of 32.93

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
MogR	Repressor of flagellar motility genes
PsrA	Oleic acid responsive regulator

NZ_CP026736.1 - Region 4 - terpene

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Location: 2,205,728 - 2,226,576 nt. (total: 20,849 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

C2I28_RS11515: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

C2I28_RS11520: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

C2I28_RS11550: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Transferases: aminotransferase

C2I28_RS11555: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Hydrolases: glucosidase hydrolase

C2I28_RS11510: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

C2I28_RS11540: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.69	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000645.3	0.63	terpene	carotenoid	Halobacillus halophilus DSM 2266
BGC0000646.5	0.48	terpene	β-carotein	uncultured bacterium
BGC0000858.3	0.47	other (other)	ectoine	Methylobacter marinus
BGC0000640.3	0.46	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000650.3	0.45	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000919.5	0.44	other (other)	phaseolotoxin	Pseudomonas syringae
BGC0000885.3	0.44	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0000648.3	0.44	terpene	carotenoid	Myxococcus xanthus
BGC0001374.5	0.43	other (other)	thienodolin	Streptomyces albogriseolus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.69	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000645.3	0.63	terpene	carotenoid	Halobacillus halophilus DSM 2266
BGC0000646.5	0.48	terpene	β-carotein	uncultured bacterium
BGC0000858.3	0.47	other (other)	ectoine	Methylobacter marinus
BGC0000640.3	0.46	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000650.3	0.45	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000919.5	0.44	other (other)	phaseolotoxin	Pseudomonas syringae
BGC0000885.3	0.44	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0000648.3	0.44	terpene	carotenoid	Myxococcus xanthus
BGC0001374.5	0.43	other (other)	thienodolin	Streptomyces albogriseolus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	C30 carotenoid	diapophytoene	C30	acyclic	none

Genes with terpene domains:

C2I28_RS11525: PT_phytoene_like

Domain 1 (6 - 281): Prenyltransferase; Phytoene synthase like

Subtype: Diapophytoene synthase

Reactions:: FPP → diapophytoene

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 25.42 out of 26.06

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 26.38 out of 27.78

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 18.25 out of 18.46

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.89 out of 20.97

Confidence: medium

Additional weak hits (1):

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.99 out of 20.01

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MogR	Repressor of flagellar motility genes
RpoN	Regulator of nitrogen assimilation and virulence

NZ_CP026736.1 - Region 5 - T3PKS

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Location: 3,003,121 - 3,044,206 nt. (total: 41,086 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

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SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

C2I28_RS15400: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Transferases: acetyltransferase methyltransferase

C2I28_RS15405: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

C2I28_RS15415: isoprenylcysteine carboxyl methyltransferase

smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

Hydrolases: glucosidase

C2I28_RS15335: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

Ligases: synthase

C2I28_RS15410: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

Translocases: transporter

C2I28_RS15360: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.54	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001831.3	0.54	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0002285.2	0.53	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001310.5	0.53	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0001962.3	0.49	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0002332.2	0.44	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.
BGC0001810.3	0.41	PKS	trehangelin	Polymorphospora rubra
BGC0002656.2	0.41	PKS	oryzanaphthopyran A, oryzanaphthopyran B, oryzanaphthopyran C, oryzanthrone A, oryzanthrone B, chlororyzanthrone A, chlororyzanthrone B	Streptacidiphilus oryzae TH49
BGC0000495.5	0.40	ribosomal	actagardine	Actinoplanes garbadinensis
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.54	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001831.3	0.54	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0002285.2	0.53	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001310.5	0.53	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0001962.3	0.49	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0002332.2	0.44	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.
BGC0001810.3	0.41	PKS	trehangelin	Polymorphospora rubra
BGC0002656.2	0.41	PKS	oryzanaphthopyran A, oryzanaphthopyran B, oryzanaphthopyran C, oryzanthrone A, oryzanthrone B, chlororyzanthrone A, chlororyzanthrone B	Streptacidiphilus oryzae TH49
BGC0000495.5	0.40	ribosomal	actagardine	Actinoplanes garbadinensis
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.52 out of 27.55

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.70 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.74 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 25.15 out of 29.38

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 25.15 out of 27.78

Confidence: strong

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.98 out of 28.19

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.57 out of 25.31

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.74 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.72 out of 27.78

Confidence: medium

Additional weak hits (2):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.51 out of 32.74

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 26.26 out of 31.73

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CatR	H2O2-responsive repressor
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
FuR variant 1	Ferric Uptake Regulator
GnfM	Regulator of nitrogen-fixation genes
MogR	Repressor of flagellar motility genes
ZuR variant 1	Zinc-responsive repressor

NZ_CP026736.1 - Region 6 - RRE-containing

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Location: 3,309,352 - 3,330,458 nt. (total: 21,107 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

C2I28_RS16850: dehydrogenase

smcogs: SMCOG1181: dehydrogenase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000585.5	0.47	ribosomal	microcin C7	Escherichia coli
BGC0001879.4	0.37	other (other)	type R and F tailocins	Pseudomonas fluorescens
BGC0000925.5	0.31	other (other)	quinolobactin	Pseudomonas fluorescens
BGC0002051.2	0.30	NRPS	antipain	Streptomyces sp.
BGC0000316.5	0.20	NRPS	capreomycin IA, capreomycin IB, capreomycin IIA, capreomycin IIB	Saccharothrix mutabilis subsp. capreolus
BGC0000084.5	0.20	PKS	laidlomycin	Streptomyces sp. CS684
BGC0002327.3	0.20	NRPS	bolagladin A, bolagladin B	Burkholderia gladioli
BGC0002336.2	0.20	NRPS	bolagladin A, bolagladin B	Burkholderia gladioli
BGC0000100.5	0.19	PKS	monensin	Streptomyces cinnamonensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000585.5	0.47	ribosomal	microcin C7	Escherichia coli
BGC0001879.4	0.37	other (other)	type R and F tailocins	Pseudomonas fluorescens
BGC0000925.5	0.31	other (other)	quinolobactin	Pseudomonas fluorescens
BGC0002051.2	0.30	NRPS	antipain	Streptomyces sp.
BGC0000316.5	0.20	NRPS	capreomycin IA, capreomycin IB, capreomycin IIA, capreomycin IIB	Saccharothrix mutabilis subsp. capreolus
BGC0000084.5	0.20	PKS	laidlomycin	Streptomyces sp. CS684
BGC0002327.3	0.20	NRPS	bolagladin A, bolagladin B	Burkholderia gladioli
BGC0002336.2	0.20	NRPS	bolagladin A, bolagladin B	Burkholderia gladioli
BGC0000100.5	0.19	PKS	monensin	Streptomyces cinnamonensis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.97 out of 26.06

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.02 out of 23.79

Confidence: medium

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 24.05 out of 32.93

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 22.97 out of 24.78

Confidence: strong

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 24.83 out of 28.14

Confidence: medium

Additional weak hits (1):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.13 out of 26.85

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Pantocin_Microcin_RRE; Location: 0 - 87; Score: 59.1

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
LexA variant 2	Repressor of DNA damage response
LexA variant 3	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes
PerR	Peroxide regulator

NZ_CP026736.1 - Region 7 - terpene

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Location: 3,703,105 - 3,724,982 nt. (total: 21,878 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

C2I28_RS18880: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acetyltransferase

C2I28_RS18815: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

C2I28_RS18830: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002173.2	0.47	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0000527.5	0.40	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0002674.2	0.40	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000557.5	0.40	ribosomal	streptococcin A-FF22	Streptococcus pyogenes
BGC0000547.5	0.40	ribosomal	salivaricin 9	Streptococcus salivarius
BGC0001788.3	0.40	ribosomal	suicin 65	Streptococcus suis
BGC0000545.5	0.39	ribosomal	ruminococcin A	[Ruminococcus] gnavus E1
BGC0000550.3	0.39	ribosomal	salivaricin G32	Streptococcus salivarius

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002173.2	0.47	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0000527.5	0.40	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0002674.2	0.40	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000557.5	0.40	ribosomal	streptococcin A-FF22	Streptococcus pyogenes
BGC0000547.5	0.40	ribosomal	salivaricin 9	Streptococcus salivarius
BGC0001788.3	0.40	ribosomal	suicin 65	Streptococcus suis
BGC0000545.5	0.39	ribosomal	ruminococcin A	[Ruminococcus] gnavus E1
BGC0000550.3	0.39	ribosomal	salivaricin G32	Streptococcus salivarius

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	hopanoid		C30	hopanoid skeleton	unknown
triterpene	polypodane		C30	polypodane skeleton	unknown

Genes with terpene domains:

C2I28_RS18835: T2TS

Domain 1 (3 - 624): Type 2 terpene synthase

Subtype: Squalene substrate

Reactions:: squalene → hopanoid; squalene → polypodane

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 24.74 out of 26.33

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 17.80 out of 18.16

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.57 out of 18.57

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 20.33 out of 20.97

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.55 out of 19.26

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.10 out of 26.06

Confidence: medium

Additional weak hits (3):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 25.17 out of 33.73

Confidence: weak

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.13 out of 29.72

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 23.64 out of 33.14

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A
MogR	Repressor of flagellar motility genes
ToxT	Regulator of MSHA biosynthesis

NZ_CP026736.1 - Region 8 - lassopeptide

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Location: 3,752,581 - 3,781,332 nt. (total: 28,752 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

C2I28_RS19145: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: glycosyltransferase

C2I28_RS19055: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

Hydrolases: hydrolase

C2I28_RS19075: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

C2I28_RS19160: isochorismatase

smcogs: SMCOG1027: isochorismatase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001356.4	0.69	ribosomal	paeninodin	Paenibacillus dendritiformis C454
BGC0002150.2	0.55	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.55	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0002312.2	0.53	ribosomal	ubonodin	Burkholderia ubonensis
BGC0001634.3	0.52	ribosomal	keywimysin	Streptomyces sp. NRRL F-5702
BGC0001552.3	0.52	ribosomal	citrulassin F	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002289.2	0.52	ribosomal	Leepeptin	Streptomyces leeuwenhoekii
BGC0001550.3	0.51	ribosomal	citrulassin D	Streptomyces katrae
BGC0000571.3	0.51	ribosomal	burhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001496.3	0.47	ribosomal	actinokineosin	Actinokineospora spheciospongiae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001356.4	0.69	ribosomal	paeninodin	Paenibacillus dendritiformis C454
BGC0002150.2	0.55	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.55	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0002312.2	0.53	ribosomal	ubonodin	Burkholderia ubonensis
BGC0001634.3	0.52	ribosomal	keywimysin	Streptomyces sp. NRRL F-5702
BGC0001552.3	0.52	ribosomal	citrulassin F	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002289.2	0.52	ribosomal	Leepeptin	Streptomyces leeuwenhoekii
BGC0001550.3	0.51	ribosomal	citrulassin D	Streptomyces katrae
BGC0000571.3	0.51	ribosomal	burhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001496.3	0.47	ribosomal	actinokineosin	Actinokineospora spheciospongiae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptide(s) for C2I28_RS19080

C2I28_RS19080

Leader:

MKKEWQIPVLETLDITMTMASTVYGPYTDEAY

Full core:

VPGKDVDKDNPGTWKRFTS

Putative macrolactam:

VPGKDVDKD

antiSMASH-DB 4.0 matches for C2I28_RS19080

100.0%

NZ_CP026736.1and 3 others:

NZ_CP064270.1 Lassopeptides: IQ863_RS06070_lassopeptideNZ_CP064275.1 Lassopeptides: IQ966_RS20505_lassopeptideNZ_CP086208.1 Lassopeptides: LJY09_RS10770_lassopeptide

Lassopeptides: C2I28_RS19080_lassopeptide

1

VPGKDVDKDNPGTWKRFTS

19

VPGKDVDKDNPGTWKRFTS

1

VPGKDVDKDNPGTWKRFTS

19

89.5%

NZ_JACYOD010000011.1:

Lassopeptides: IGH99_RS07520_lassopeptide

1

VPGKDVDKDNPGTWKRFTS

19

VPGK+VDKDNPGTW+RFTS

1

VPGKEVDKDNPGTWRRFTS

19

73.7%

NZ_JADBDR010000009.1and 1 other:

NZ_JMLS01000021.1 Lassopeptides: BP97_RS40275_lassopeptide

Lassopeptides: GGC63_RS20275_lassopeptide

1

VPGKDVDKDNPGTWKRFTS

19

VPGK VD +NP TWKRF S

1

VPGKPVDPNNPSTWKRFNS

19

with 11 more sequence matches

Only the first 10 are displayed here.

68.4%

NZ_FNJU01000006.1:

Lassopeptides: BLQ56_RS23205_lassopeptide

1

VPGKDVDKDNPGTWKRFTS

19

VPG +VDK++PGTW RF S

1

VPGVEVDKNDPGTWNRFDS

19

63.2%

NZ_LTAO01000036.1:

Lassopeptides: AZF04_RS21035_lassopeptide

1

VPGKDVDKDNPGTWKRFTS

19

VPG+ VD ++ GTW RFTS

1

VPGEGVDGNDSGTWDRFTS

19

57.1%

NZ_CP095462.1:

Lassopeptides: MUG87_RS00750_lassopeptide

1

VPGKDVDKDNPGTWKRFTS

19

VPGK VD +NPGTWKRFTS

10

VPGKPVDPNNPGTWKRFTS

28

53.6%

NZ_CP033044.1:

Lassopeptides: D9829_RS05775_lassopeptide

1

VPGKDVDKDNPGTWKRFTS

19

VPGK+VD +NPGTW+RFTS

10

VPGKEVDPNNPGTWRRFTS

28

53.6%

NZ_CP085708.1:

Lassopeptides: LIT32_RS13255_lassopeptide

1

VPGKDVDKDNPGTWKRFTS

19

VPG++VD +NPGTWKRFTS

10

VPGEEVDPNNPGTWKRFTS

28

50.0%

NZ_PKLB01000002.1:

Lassopeptides: CYJ36_RS23375_lassopeptide

1

VPGKDVDKDNPGTWKRFTS

19

VPG VD +N GTWKRFTS

10

VPGTPVDPNNQGTWKRFTS

28

46.4%

NZ_JARFVC010000022.1:

Lassopeptides: PY093_RS13835_lassopeptide

1

VPGKDVDKDNPGTWKRFTS

19

VPG++ D +NP TWKRFTS

10

VPGEEEDPNNPQTWKRFTS

28

42.9%

NZ_FOLV01000014.1and 1 other:

NZ_KN050811.1 Lassopeptides: BR72_RS26555_lassopeptide

Lassopeptides: BM211_RS26025_lassopeptide

2

PGKDVDKDNPGTWKRFTS

19

PG VD NP TW RFTS

11

PGHPVDPKNPDTWNRFTS

28

42.9%

NZ_JAOTPO010000006.1:

Lassopeptides: N7Z68_RS10450_lassopeptide

1

VPGKDVDKDNPGTWKRFTS

19

VPG+ VD ++P TW RFTS

10

VPGESVDPNDPETWDRFTS

28

39.3%

NZ_FPAF01000001.1and 1 other:

NZ_JAQOTJ010000004.1 Lassopeptides: PLX19_RS07890_lassopeptide

Lassopeptides: BM350_RS25945_lassopeptide

2

PGKDVDKDNPGTWKRFTS

19

PG VD +P TW RFTS

11

PGHPVDPKDPNTWNRFTS

28

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.88 out of 27.78

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.57 out of 25.88

Confidence: medium

Additional weak hits (1):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.77 out of 32.74

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

C2I28_RS19080 - Class II: Cleavage pHMM score: -12.10; RODEO score: 15; Monoisotopic mass: 2128.1 Da; Molecular weight: 2129.3 Da; Number of bridges: 0

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 14 - 95; Score: 91.7; RRE type: Thurincin_rSAM_RRE; Location: 31 - 91; Score: 27.1; RRE type: Stand_Alone_Lasso_RRE; Location: 6 - 85; Score: 56.7; RRE type: PqqD_RRE; Location: 13 - 85; Score: 32.1

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
PsrA	Oleic acid responsive regulator

NZ_CP026738.1 - Region 1 - T2PKS

Download region GenBank file

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Location: 1 - 67,944 nt. (total: 67,944 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: methyltransferase

C2I28_RS26670: methyltransferase

smcogs: SMCOG1089: methyltransferase

C2I28_RS26715: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: dehalogenase

C2I28_RS26705: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

Lyases: cyclase

C2I28_RS26700: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Ligases: ligase synthase

C2I28_RS26655: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

C2I28_RS26660: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

C2I28_RS26680: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000272.3	0.59	PKS	spore pigment	Streptomyces collinus
BGC0000271.5	0.56	PKS	spore pigment	Streptomyces avermitilis
BGC0001723.5	0.54	PKS	oviedomycin	Streptomyces antibioticus
BGC0000234.5	0.54	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000275.4	0.54	PKS	tetracenomycin C	Streptomyces glaucescens
BGC0001513.3	0.54	PKS	anthrabenzoxocinone	Actinobacteria bacterium
BGC0002000.4	0.53	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000196.4	0.53	PKS	AQ-256, AQ-270a, AQ-284a	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001197.5	0.52	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0002514.3	0.52	PKS	julichrome Q6.6	Streptomyces sampsonii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000272.3	0.59	PKS	spore pigment	Streptomyces collinus
BGC0000271.5	0.56	PKS	spore pigment	Streptomyces avermitilis
BGC0001723.5	0.54	PKS	oviedomycin	Streptomyces antibioticus
BGC0000234.5	0.54	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000275.4	0.54	PKS	tetracenomycin C	Streptomyces glaucescens
BGC0001513.3	0.54	PKS	anthrabenzoxocinone	Actinobacteria bacterium
BGC0002000.4	0.53	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000196.4	0.53	PKS	AQ-256, AQ-270a, AQ-284a	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001197.5	0.52	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0002514.3	0.52	PKS	julichrome Q6.6	Streptomyces sampsonii

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 21.55

Confidence: medium

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 21.55

Confidence: medium

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 21.55

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.09 out of 26.06

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 23.44 out of 24.78

Confidence: strong

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 23.31 out of 24.78

Confidence: strong

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 21.27 out of 23.43

Confidence: medium

Additional weak hits (8):

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.09 out of 29.51

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 25.52 out of 33.14

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.71 out of 23.43

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 20.08 out of 30.22

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.85 out of 32.74

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 17.35 out of 30.12

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 16.52 out of 30.12

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 24.94 out of 33.14

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

TFBS Finder

NRPS/PKS substrate predictions

C2I28_RS26680: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (19..407): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: SSYMYGLG-NSMFGLSAGEYEGIGSTEMLHIYIC
Nearest Stachelhaus code(s):: Aib DLGFLAGVFK (29% 8Å match); Ala DLFVLAGCIK (26% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 67944
Starter unit(s):: acetyl-CoA (Score: 0.0; E-value: 0)

C2I28_RS26655: KS (Score: 410.5; E-value: 1.7e-125)
C2I28_RS26660: CLF (Score: 323.5; E-value: 4.1e-99)
C2I28_RS26695: CYC C5-C14/C3-C16 (Score: 290.5; E-value: 2.6e-89)
C2I28_RS26700: CYC C9-C14 (Score: 152.9; E-value: 1.2e-47)

TFBS definitions

Regulator	Description
ArcA	Repressor of carbon oxidation
BldD	Development and antibiotic global regulator
LexA variant 2	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes