LOCUS       NZ_KZ679082            11368 bp    DNA     linear   CON 17-JAN-2024
DEFINITION  Streptomyces sp. CS149 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ679082
VERSION     NZ_KZ679082.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS149
  ORGANISM  Streptomyces sp. CS149
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7754255)
  AUTHORS   Malmierca,M.G., Perez-Victoria,I., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Cooperative Involvement of Glycosyltransferases in the Transfer of
            Amino Sugars during the Biosynthesis of the Macrolactam Sipanmycin
            by Streptomyces sp. Strain CS149
  JOURNAL   Appl Environ Microbiol 84 (18), e01462-18 (2018)
   PUBMED   30006405
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754255)
  AUTHORS   Olano,C., Malmierca,M.G., Mendez,C. and Salas,J.A.
  TITLE     Characterization of the biosynthesis gene cluster for the
            macrolactam sipanmycins: involvement of four glycosyltransferases in
            the transfer of two aminosugars
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7754255)
  AUTHORS   Olano,C., Malmierca,M.G., Mendez,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2018) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 556.87x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/17/2024 11:43:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,915
            CDSs (total)                      :: 6,834
            Genes (coding)                    :: 6,691
            CDSs (with protein)               :: 6,691
            Genes (RNA)                       :: 81
            rRNAs                             :: 12, 7 (16S, 23S)
            complete rRNAs                    :: 12, 7 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 52 of 144
            Pseudo Genes (incomplete)         :: 108 of 144
            Pseudo Genes (internal stop)      :: 12 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:40:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7472498
            Orig. end                         :: 7483866
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ679082.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Professor JA Salas
            laboratory.
FEATURES             Location/Qualifiers
     region          1..11368
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="30"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11368
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11368
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6368](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6368
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            217..1284
                     /locus_tag="C6W96_RS33330"
                     /old_locus_tag="C6W96_33335"
     CDS             217..1284
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955794.1"
                     /locus_tag="C6W96_RS33330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_33335"
                     /product="iron ABC transporter permease"
                     /protein_id="WP_107414526.1"
                     /transl_table=11
                     /translation="MTFVDTAPGPARPARRTLRAPGVRIGGLALLVAVVACAGLFAGRG
                     VTPEGVRAVLLGAGDDPTAEHIVLRLRLPRVLVALVAGACLGVAGLVLQSALRNPLAGP
                     EVTGVTPGAVLGAVTATGLGLAGWESPTAVVVAACVGGFAGAGLLWLLAGRDRGDPEQT
                     AVYGVLVSAVLAGLTAVVLLVAPGELGSVVQWLIGSTEGRVWQHWNLLWPWAAFWGAAA
                     WLLAGPLTLLRCGDEQAAAAGLSTGRGRGAALVCAVALTAGAVSAVGALGFVGLLVPHL
                     ALAVFGADLRVTLPGAALLGAAVVCGADAAAQLLSRLLATALDADRLTLPVGALTTCVG
                     AGLLLVVARRRADER"
     misc_feature    1298..1309
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: weak, score: 17.94"
                     /tool="antismash"
     gene            1323..2168
                     /locus_tag="C6W96_RS33335"
                     /old_locus_tag="C6W96_33340"
     CDS             1323..2168
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059071.1"
                     /locus_tag="C6W96_RS33335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_33340"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_107414527.1"
                     /transl_table=11
                     /translation="MNSGVPKGPDVVRAEGLAFGYPGRPVLRGVDLRIRAGELVALIGL
                     NGCGKSTFLKLAAGLLTPSAGRVLLDGDDVARLPRRTAARRVALLHQSAPSVPGLTVRQ
                     LVRQGRYAARGPLGMLREGDDEVTSRALADVGVTAWADRPVDALSGGERQRVRLAMALA
                     QDAPVLFLDEPTTYLDLRHQLEVLQTVVRLRAERRLTVVMVLHDLNHAARFADRIVALR
                     EGRIAADGPPAEVVTRHLLAEVLGVDGRVGTDDVGGWPVCYPDHPLDPLQLLRNENHIH
                     "
     gene            2237..2371
                     /gene="amiA"
                     /locus_tag="C6W96_RS35660"
     CDS             2237..2371
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508296.1"
                     /locus_tag="C6W96_RS35660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="streptamidine family RiPP"
                     /protein_id="WP_006129278.1"
                     /transl_table=11
                     /translation="MEQNKVFSPIADQGQLAHLSATHSNALVENPFDDAVEADTAAEK"
     misc_feature    complement(2244..2258)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.28"
                     /tool="antismash"
     gene            2519..3451
                     /locus_tag="C6W96_RS33340"
                     /old_locus_tag="C6W96_33345"
     CDS             2519..3451
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059073.1"
                     /locus_tag="C6W96_RS33340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_33345"
                     /product="hypothetical protein"
                     /protein_id="WP_107414528.1"
                     /transl_table=11
                     /translation="MTRSRLAPGAGIVTVPGAAPVLRTSGGEFLRIDTGGVTAEALVRR
                     LAGEEAPDEDPQAAELARLVEAFEAGGHAVSAPPRAPLAGRTVNVLGDPLLAGPVARCA
                     GAEGAEVQPITPDRVAELAQAVPDHRAEGRVAVVWCLDSPVPEGLWDAADGLPDRRIAW
                     LRCHREGSHSWTEPLAAAPGDVTSRHVRLRRLAATPAHRELAAYWAGHRTPDTGPHPTG
                     ASASLIAALLTADLIAWAEAEATGGGGGVGGTGRQSGGLPARRRLRRIDLRDLTFTEHP
                     VLPVPDVAPLPAPAPAGTRSRSDTAGARP"
     gene            3448..5004
                     /locus_tag="C6W96_RS33345"
                     /old_locus_tag="C6W96_33350"
     CDS             3448..5004
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377868.1"
                     /locus_tag="C6W96_RS33345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_33350"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_107414529.1"
                     /transl_table=11
                     /translation="MNTRTVSVLTPDGTPLATDVCLPDGDRPRPAVLIRTPYGRDAHRA
                     ELRGWAAHGFAALAQDVRGRHGSPGEWHPYRDHERTDGAATVAWVRAQTWSNGEVVAVG
                     ASYAAYCALVTALDAPGAAPGPVHREGVPDAVIAAVPALGLTETAREPGGPERLWARAG
                     WWAAHGDRSDSDPDALERALADDPRLLEHLPVARLAERLATGLGRELPSWPRLWADRQR
                     GRLVALGSAARLPLLAVGGTRDPFAEDTVALWHGWGGPARLLLGPWGHHLTADRSAPAR
                     ARVNLGALYVRWARAALAGRLEPTRHGVIALGDSGHWHSTHRADSPPETERRWPFGSPT
                     GLRLLHGAEFSADPERPVRSDDLAVPVDGGPADRCLLLSPPLPRPLDLAGAAVARINAT
                     ADTPSADWAVRLTALDPSGRADPLAFGIVRRTGPPGEAADITVPLGTLGRRLPAGTRLR
                     AEIAGHHFPAHARNPHTGDDPVTATRLAPSRRAVNPRGSALHLPVVARRRYVEPVPEIC
                     R"
     gene            5001..6368
                     /locus_tag="C6W96_RS33350"
                     /old_locus_tag="C6W96_33355"
     CDS             5001..6368
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059075.1"
                     /locus_tag="C6W96_RS33350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_33355"
                     /product="YcaO-like family protein"
                     /protein_id="WP_107415154.1"
                     /sec_met_domain="YcaO (E-value: 4.8e-45, bitscore: 153.3,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSVLPLEALVDPVSGIVRAVAPVEHPAGAPPRYTAMTADVADAR
                     RLGAWPADRVSLGTTFGDPRGAWIAAVAEAVERYCGNRLPPPGHPQEPRRATAAELTAE
                     GARLYGPGTLPAYAPWQYGRPGFPYAELTPDTPALWTRAVENGEPCWAPVALTHLNWRQ
                     GELRSLPRTHHLNYAGIATGQGLDDAVERGLLEVVERDALELWWHLDGPTRGIDPASVP
                     GLTEDLAGCGLDVHIVEMPSEFAPCVAALVHDPGRGMYAAGFACRYDPAEAARKAVLEA
                     VHTWVFTHGTVDAGGWVYRAIEAGMLARGLYLDHRADRRYLDDCGPEFAAVRDLGAHVQ
                     VWLDDRMAPLARRFTEPALGVVPVTAIAPGSRTALDAALAAGGHRVITADLTTEDVAET
                     MLRVARVLVSGLIPNAPAAFGYFGCPRFAEAALARGWRAQRPTRPEDFTLAPPPHM"
     gene            6368..7849
                     /locus_tag="C6W96_RS33355"
                     /old_locus_tag="C6W96_33360"
     CDS             6368..7849
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129282.1"
                     /locus_tag="C6W96_RS33355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_33360"
                     /product="nitroreductase"
                     /protein_id="WP_107414530.1"
                     /transl_table=11
                     /translation="MERASMPDTPDRPPVPARGSGAVPPAASDLLRAALADARSPRVPE
                     LAPYAPERPFPWNAPVTANAGEPGAVIDLDRVLRLSLAAPVGTPGRLRPVPSAGALHPV
                     RAHLLLGQGCSLPPGRYAHDPRTHRAHLRGPAPDGVPPGALVVLTVVASRTVAHYGHRA
                     WPLLLLDTGHAAAALALAGATTTDVLVSLDADGSLLSAAAGLPDAPDWQDIWPGTEPEL
                     PLAAVLLTPPGGPSGIDPPLRAWAALPRGSAPTPRPGADTAPPRELAAARHLLHHIAEA
                     PGRSGGTWHPASRPGQVTDEALETRRSAPAEDLNRPPGKELLSRILATAENVRPDGLAW
                     TVAVGNAAPALYAAAPGGPGLDVRAVGDARPALAHWAAGQQWIATSGAVLLAHGCPDDA
                     SHATVRGSHLVAGYAAGAAQAHATALGLRSRPIGSWQQADLGAAFGDTPGRDWIVHGLA
                     LGAPPTAADSPVTTEATPATPPAAPAAPSGEEERP"
     gene            7846..9585
                     /locus_tag="C6W96_RS33360"
                     /old_locus_tag="C6W96_33365"
     CDS             7846..9585
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964397.1"
                     /locus_tag="C6W96_RS33360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_33365"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_107414531.1"
                     /transl_table=11
                     /translation="MMLHPELLRAARTVRRPLGLATALLAAVTATHLAQAVLLALVLSR
                     IAQGETGALAPLLAAVIAVVLVRAALGRAQRLTAVTAGATVRVRLRDTLMERLGALGPT
                     AITGARAGAVRATLVDGVEGVDAYLARYLPQLLITAAVPPLLLVAVSLVEPYAALALAP
                     ALLLALFGPRAWDRLLARRGKEHWDTYEALAADYLEALQGMPTLRNAGAVGRVRERLER
                     RSTALHRSTVAKLRVSLVDTGLTDLAVQGGTVAAVLVACSSAATGRTAATGTYLLLLLA
                     SECFRPVRDLSREWHAGYLGVSAADGIAALRTAEAAVPDRGTKTAAWENAPEIRFQDVH
                     FTHPGGARPALGGVSFTAPAGRTTAVVGPSGAGKSTVLSLLLRQRDPDGGRVTVSGTDT
                     AAYTLASLRRGIAVVSQETYLFHATIAENLRIARPAATDEDLRTAARTAGIDEEITRLP
                     DGYATLVGERGATLSGGQRQRLALARALLADAPVLILDEATSAVDERGQARIVRALATA
                     GRGRTCVVVAHRLDAVRHADHIVVLDAGRVAAAGDHTTLLAGGGVYASLVAAGHTAEEE
                     RAA"
     misc_feature    11273..11287
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 17.84"
                     /tool="antismash"
ORIGIN
        1 cgcgatcgtc gtgctctgcg ccgaccagcc ggcccggctc gcgctcgccc cggtcgagac
       61 gcccgtcggc gcctggaccg ccctggtcgg cgtgcccgtc ggggtcgtgc tgctgaaacg
      121 ggggcggcgg cccgcccggg accgggggcc cgtgcccctg cccgcgccgg cgactccgtc
      181 ggacgccccc gcacccgtac tgcggaaggc ggaacgatga ccttcgtcga caccgcgccc
      241 ggaccggccc gccccgcccg ccggaccctg cgcgcgccgg gcgtccggat cggcgggctg
      301 gcgctgctcg tcgccgtcgt cgcctgcgcc ggactcttcg ccggccgggg cgtcaccccc
      361 gaaggcgtgc gggccgtgct gctcggggcc ggcgacgacc ccaccgccga gcacatcgtg
      421 ctccggctcc ggttgccccg cgttctggtg gccctggtcg ccggggcctg cctcggagtg
      481 gccggactcg tgctccagtc ggccctgcgc aacccgctgg ccggacccga ggtcaccggt
      541 gtgacacccg gcgcggtact cggcgcggtg accgcgaccg gcctcggcct cgcgggctgg
      601 gagtccccca cggccgtggt cgtcgcggcg tgcgtcggcg gcttcgccgg agcgggactg
      661 ctgtggcttc tcgccgggcg ggaccggggc gacccggaac agaccgccgt ctacggggtc
      721 ctcgtctcgg cggtgctcgc cggactcacc gcggtcgtcc tcctggtcgc ccccggtgaa
      781 ctcggcagcg tcgtccagtg gttgatcggc tccaccgagg gccgggtctg gcagcactgg
      841 aatctgctct ggccctgggc cgccttctgg ggcgcggcgg cctggctgct cgccgggccg
      901 ctgaccctgc tgcgctgcgg cgacgaacag gccgccgccg cgggcctgtc caccggccgg
      961 ggcaggggag cggcgctcgt gtgcgcggtg gcgctgacgg ccggcgccgt gtcggcggtg
     1021 ggcgccctcg ggttcgtcgg gctgctggtg ccccatctgg ctctggccgt cttcggcgcc
     1081 gatctgcgcg tcacgcttcc gggggccgca ctgctcgggg cggcggtggt gtgcggggcg
     1141 gacgccgcgg cccaactgct gtcccggctg ctggccaccg cgctcgacgc ggaccggctg
     1201 accctcccgg tcggtgccct gaccacctgt gtgggggcgg ggctgctgct ggtcgtggca
     1261 cggcgacggg cggacgagag atgaggagga gaacagtgga aacgagcgcg acggagagcg
     1321 cgatgaacag tggggtgccg aaaggcccgg acgtcgtccg ggcggagggg ctggccttcg
     1381 gctaccccgg acgcccggtg ctgcgcgggg tggacctgcg catccgggcc ggtgaactgg
     1441 tcgccctgat cgggctgaac ggctgcggga agagcacctt cctcaagctc gccgccggac
     1501 tgctcacccc gagcgccggg cgcgtcctgc tcgacggcga cgacgtggcg cggctgcccc
     1561 gccgcaccgc cgcccggcgc gtggcactgc tccaccagtc ggcgccctcc gtgccgggac
     1621 tgaccgtacg tcaactcgta cgtcaggggc gctatgccgc ccgggggccg ctcgggatgc
     1681 tgcgggaggg cgacgacgag gtgacgtccc gggcgctcgc ggacgtcggt gtcaccgcct
     1741 gggccgaccg gcccgtggac gcgctgtccg gaggcgaacg ccagcgtgtc cggctggcca
     1801 tggcgctggc ccaggacgcg ccagtgctgt tcctcgacga gccgaccacc tatctcgatc
     1861 tgcggcacca gctggaggtg ctccagacgg tcgtacggct gcgcgcggag cgccggctga
     1921 cagtggtgat ggtgctgcac gacctcaacc acgccgcccg gttcgccgat cggatcgtcg
     1981 cgctccggga aggccgcatc gccgccgacg ggcccccggc cgaggtggtc acccggcatc
     2041 tgctggccga agtcctcggc gtggacggcc gggtggggac cgacgacgtg ggcggctggc
     2101 ccgtctgtta cccagatcac cctctcgacc cgctccagct attgcggaat gaaaatcata
     2161 ttcattagtg tctgtgctgc ggcacgcacc aacgccgcgg accacactca accgatgtga
     2221 atggaggctt caactcatgg agcagaacaa ggtgttcagc ccgatcgccg accagggtca
     2281 gctcgctcac ctctccgcca cccactcgaa cgccctcgtc gagaacccct tcgacgacgc
     2341 ggtcgaggcc gacaccgcgg ccgagaagta agcgcctcct gccgctgaag gcaggtctcc
     2401 cgcgtggcgc gggggcgggc ccgcccggtg acaacccggg cgggcccgcc ccgttcggcc
     2461 cgagcatccc gtccctgcct tccctgcctt ctccccacct tccgacccgg aggattccgt
     2521 gacccgcagt cgactcgctc ccggtgccgg gatcgtcacc gttcccggcg ccgcccccgt
     2581 gctccggacc tccggagggg agttcctgcg catcgacacc ggcggcgtca ccgcggaagc
     2641 gttggtgcgc cggctggcgg gagaggaagc gccggacgaa gaccctcagg ccgccgaact
     2701 ggcgcggctc gtcgaggcgt tcgaggccgg tgggcacgct gtgtcagccc cgccccgggc
     2761 cccgctcgcc ggccgcaccg tcaacgtcct cggcgacccc ctgctcgccg ggcctgtggc
     2821 gcgctgtgcc ggggcggaag gggccgaggt ccagccgatc accccggatc gggtcgccga
     2881 gctcgcccag gcggttccgg accaccgcgc cgaaggtcgc gtcgccgtgg tgtggtgcct
     2941 ggactcgccc gtgcccgaag ggctctggga cgcggcggac gggctgccgg atcgacgtat
     3001 cgcctggctg cgctgtcacc gggagggctc ccacagctgg accgagcccc ttgccgcggc
     3061 cccgggagac gtcacctccc ggcatgtccg cctccgcagg ctcgcggcca cccccgccca
     3121 tcgcgaactc gccgcgtact gggccggaca ccgcaccccc gacaccggac cgcaccccac
     3181 cggggcatcc gccagcctga tcgccgcgct gctcaccgcc gatctgatcg cctgggccga
     3241 agccgaagca acaggcgggg gcgggggcgt gggcggtacg gggcggcagt cgggcgggct
     3301 tccggcccgc cgccggctgc gccggatcga tctgcgcgat ctcaccttca ccgaacaccc
     3361 cgtgctcccc gttccggacg tcgcgccgct gcccgcgccg gcaccggcgg ggacgcgttc
     3421 acggtccgac accgctgggg cccgcccgtg aacacccgta ccgtgtccgt cctcacgccg
     3481 gacggcaccc cgctcgccac cgacgtatgc ctcccggacg gggaccgccc ccgccccgcc
     3541 gtcctgatcc gcaccccgta cggtcgcgac gcccaccgcg ccgagctgcg cggctgggcg
     3601 gcgcacggat tcgccgccct cgcccaggac gtacgcggcc gccacggctc accgggggag
     3661 tggcacccgt accgggacca cgagaggacg gacggcgcgg ccacggtcgc ctgggtgcgc
     3721 gcacagacgt ggagcaacgg agaggtcgtc gccgtcggcg cctcctacgc cgcgtactgc
     3781 gccctcgtca ccgcactcga cgcccccggc gctgctcccg gtcccgtaca ccgcgaagga
     3841 gtccccgacg ccgtcatcgc cgccgttccc gccctcgggc tcaccgagac cgcgcgcgag
     3901 cccggggggc cggagcggtt gtgggcccgg gccggctggt gggcggccca cggcgaccgg
     3961 agcgactccg acccggacgc gctggagcgc gcgctggccg atgacccccg gctgctcgag
     4021 cacctcccgg tcgcccgact ggcggagcgg ctggccacgg gtctcgggcg tgaactgccg
     4081 tcctggcccc ggctctgggc cgaccggcaa cgcggccgcc tcgtcgcgct cggctccgcc
     4141 gcccgcctac cgctgctcgc ggtcggcggc acccgcgacc ccttcgccga ggacaccgtg
     4201 gccctctggc acggctgggg tggaccggcg cgtctgctgc tcggcccctg ggggcaccac
     4261 ctcaccgccg accggtccgc acccgcccgc gcgcgtgtga acctcggtgc cctgtacgta
     4321 cggtgggccc gcgccgcgct cgccgggcgc ctggagccga cccgccacgg ggtcatcgcc
     4381 ctcggcgaca gcggccattg gcacagcacc caccgggcgg attcccctcc cgagacagag
     4441 cgccgctggc ccttcggttc acccaccggt ctacggcttc tccacggagc cgagttcagc
     4501 gccgacccgg agcgccccgt gcgctccgac gacctcgccg tcccggtcga cggcggcccg
     4561 gccgaccgct gtctgctgct ctcgccgccg ctgccccgcc ccctcgacct ggcgggggcc
     4621 gccgtcgccc ggatcaacgc caccgccgac acaccgtcgg ccgactgggc cgtacgcctg
     4681 accgcgctcg acccgtccgg ccgggccgac ccgctcgcct tcggcatcgt ccggcgcacc
     4741 ggcccgcccg gcgaagccgc ggacatcacc gtgccgctcg gcaccctcgg gcgcaggctg
     4801 cccgccggga ccaggctgcg ggccgagatc gccggccacc acttccccgc ccacgcccgc
     4861 aacccgcaca cgggcgacga cccggtcacg gccacccggc tcgccccgtc ccgccgcgcc
     4921 gtgaaccccc ggggcagcgc cctccacctg cccgtcgtcg cccgacgccg ctacgtcgag
     4981 cccgtaccgg agatatgccg ttgaccagcg tcctgcccct cgaagccctc gtcgaccccg
     5041 tcagcggaat cgtccgcgcc gtcgcccccg tcgagcaccc cgccggtgcg ccgccccgct
     5101 acaccgcgat gaccgccgac gtcgccgacg cccgccgtct cggcgcctgg cccgccgacc
     5161 gggtctcgct cggcaccacg ttcggcgacc cccggggcgc ctggatcgcc gcggtggccg
     5221 aggccgtgga gcgctactgc ggcaaccgcc tcccgccgcc cggccacccc caggagcccc
     5281 ggcgggccac cgcggccgag ctgaccgccg agggcgcccg cctctacggt cccggcacgc
     5341 tgccggcgta cgcaccgtgg cagtacggcc gcccgggctt cccgtacgcc gaactcaccc
     5401 ccgacacacc ggcgctgtgg acccgagccg tcgagaacgg cgaaccctgc tgggcccccg
     5461 tcgccctgac ccacctcaac tggcgccagg gagagctgcg ttcactaccg cgcacccacc
     5521 acctcaacta cgcgggcatc gccaccggac agggcctcga cgacgccgtc gagcgcgggc
     5581 tcctcgaagt cgtcgagcgc gacgcgctgg agctgtggtg gcacctggac ggacccaccc
     5641 gaggcatcga cccggcgagc gtccccgggc tcaccgagga cctggccggc tgcgggctgg
     5701 acgtgcacat cgtggagatg ccctccgagt tcgccccctg tgtcgcggcc ctcgtgcacg
     5761 accccggacg cgggatgtac gcggcggggt tcgcctgccg ttacgacccg gcggaagcgg
     5821 cccgcaaggc cgtcctggag gccgtgcaca cctgggtgtt cacacacggc acggtggacg
     5881 cgggcgggtg ggtgtaccgg gcgatcgagg ccgggatgct cgcccgcggc ctctacctcg
     5941 accaccgggc cgaccggcgc tatctcgacg actgcgggcc cgagttcgcc gccgtccgcg
     6001 acctcggcgc ccatgtccag gtctggctgg acgaccggat ggccccgctc gcccgccgct
     6061 tcaccgaacc cgccctgggc gtcgtcccgg tcaccgcgat cgcacccggc agccgcaccg
     6121 cgctcgacgc ggcactcgcc gccgggggcc accgggtcat caccgcggac ctcacgacgg
     6181 aggacgtcgc cgagaccatg ctgcgagtcg cccgggtcct cgtctccggg ctgatcccca
     6241 acgccccggc agccttcggc tacttcggct gcccgcgctt cgcggaagcg gcgctcgccc
     6301 ggggctggcg cgcccaacgg ccgacccgcc ccgaggactt caccctcgcc ccgcccccgc
     6361 acatgtgatg gagcgcgcca gcatgcccga cacgccggac cgtccccccg tgcccgctcg
     6421 cgggtcgggc gccgtcccgc ccgccgcttc ggacctcctg cgcgcggcac tcgcggacgc
     6481 ccggtcgcca cgcgttccgg agctcgcccc gtacgcgccg gagcgaccgt ttccctggaa
     6541 cgcgcccgtg accgcgaacg ccggcgaacc gggggccgtg atcgacctgg accgtgtgct
     6601 gcggctctcc ctggccgcgc ccgtcggcac accgggccgg ctgcgacccg ttccctcggc
     6661 gggcgccctg cacccggtgc gtgcccacct cctgctcggg cagggctgtt cgctgccgcc
     6721 cgggcgctac gcccacgacc cgcgcaccca ccgcgcccac ctgcgcggcc cggcccccga
     6781 cggcgtaccc ccgggcgccc tcgtcgtcct caccgtcgtc gcgtcccgga ccgtcgccca
     6841 ctacggtcac cgggcctggc cgctgctgct gctcgacacg gggcatgccg ccgccgccct
     6901 cgcgctggcc ggggccacga ccaccgatgt gctggtctcc ctcgacgcgg acggctccct
     6961 cctgtccgcc gccgccggac tgcccgacgc cccggactgg caggacatct ggcccggcac
     7021 cgagccggaa ctacccctcg cggccgtcct gttgacgcct ccgggcggcc cctccggcat
     7081 cgatccgccg ctgagagcct gggccgcgct gccgcgcggc tcggccccga ctccgcgacc
     7141 aggagccgac accgcaccac cccgtgaact ggccgcggca cggcacctgt tgcaccacat
     7201 cgccgaagcg cccgggcggt ccggcggcac ctggcacccg gcgtcgcgcc ccggacaggt
     7261 gacagacgag gccctggaga cgcgccgcag cgcccctgcc gaagatctga accgcccgcc
     7321 cggcaaggag ctgctgtccc ggatcctggc caccgccgag aacgtgcggc cggacgggct
     7381 cgcgtggacc gtggccgtgg gcaatgcggc cccagccctg tacgcagcgg ctcccggcgg
     7441 gccgggcctc gacgtccgcg ccgtgggcga cgcgcgcccc gccctcgccc actgggccgc
     7501 gggccagcag tggatcgcga cctcgggcgc ggtgctcctg gcccacggct gccccgatga
     7561 cgcctcgcac gccaccgtcc gtggcagcca cctcgtcgcc ggatacgcgg ccggcgcggc
     7621 tcaggcgcac gccaccgcac tgggcctgcg ctcacgcccc atcggctcct ggcaacaggc
     7681 ggacctgggc gcggcgttcg gcgacacccc cggacgcgac tggatcgtgc acgggcttgc
     7741 cctcggcgca cccccaacgg cagcggactc cccggtcacg accgaagcca ctccagccac
     7801 cccacccgcc gccccggcgg ccccgtccgg cgaggaagaa cgcccatgat gctccacccc
     7861 gaactcctgc gcgccgcgcg caccgtgcgg cgcccgctcg gcctcgccac cgcgctcctc
     7921 gcggcggtca ccgcaaccca cctcgcccag gccgtcctcc tcgcgctcgt gctctcccgg
     7981 atcgcgcagg gagagacggg cgccctcgcc cccctgctcg cggccgtgat cgccgtcgtc
     8041 ctggtccgcg ccgccctggg ccgcgcccag cgcctgaccg ccgtcacggc cggcgccacc
     8101 gtacgggtga ggctgcgcga caccctgatg gagcggctcg gcgcgctcgg ccccaccgcg
     8161 atcaccggtg cgcgggccgg cgccgtacgg gccaccctgg tggacggggt cgagggcgtc
     8221 gacgcgtacc tcgcgcgcta tctgccccaa ctcctcatca ccgccgccgt accgccgctg
     8281 ctgctcgtcg ccgtctctct cgtcgagccg tacgcggccc tcgccctcgc gcccgccctg
     8341 ctgctcgccc tcttcgggcc gcgcgcgtgg gaccggctgc tggcgcgacg cggcaaggaa
     8401 cactgggaca cctacgaagc cctcgccgcc gactatctgg aagccctcca ggggatgccg
     8461 acgctgcgga acgccggtgc cgtggggcgc gtacgggaac gcctggagcg gcggtcgacc
     8521 gccctgcacc ggagcaccgt cgccaaactg cgggtctcgc tcgtcgacac cggcctcacc
     8581 gacctggccg tccagggcgg cacggtcgcc gccgtcctcg tggcgtgctc ctcggccgcc
     8641 accggccgca ccgccgccac cggcacctac ctcctgctgc tgctcgcctc cgaatgcttc
     8701 cgccccgtcc gcgacctctc ccgcgaatgg cacgcgggct acctcggggt gtcggcggcc
     8761 gacggcatcg ccgccctgcg gaccgccgag gccgccgtcc cggaccgggg caccaagacc
     8821 gccgcctggg agaacgcgcc cgaaatccgt ttccaggacg tgcacttcac ccacccgggg
     8881 ggtgcccggc ccgccctcgg cggcgtgtcc ttcacggccc ccgccgggcg caccaccgcc
     8941 gtcgtcggcc cgtccggcgc gggcaagtcg accgtgctga gccttctgct gcggcagcgg
     9001 gatccggacg ggggccgcgt caccgtgtcc ggcacggaca ccgccgcgta caccctcgcc
     9061 tcgctgcgcc ggggcatcgc cgtggtctca caggagacct acctcttcca cgccacgatc
     9121 gcggagaacc tccgcatcgc ccgcccggcc gccacggacg aagacttgcg caccgccgcc
     9181 cgcaccgccg ggatcgacga ggagatcacc cggctgccgg acggctacgc caccctcgtc
     9241 ggcgaacgcg gcgccacgct ctccggtgga cagcgccaac ggctcgccct ggcacgggcg
     9301 ttgctcgccg acgccccggt cctgatcctc gacgaggcca ccagcgccgt cgacgaacgc
     9361 ggccaggccc ggatcgtccg ggcgctggcg acggccggcc gcggccgcac ctgcgtcgtc
     9421 gtcgcccacc ggctcgacgc cgtccgccac gccgaccaca tcgtcgtcct cgacgcgggc
     9481 cgggtggccg cggcgggcga ccacaccacc ctgctcgcgg gcggcggcgt ctacgcctcg
     9541 ctcgtcgcgg ccggacacac cgcagaggaa gagcgcgccg catgaccccg atcccggccc
     9601 cgaccggcgc cctgcgctcc ctcctcccgg tcctcggcgc gcaccgcgcc accgtgctcc
     9661 gcacctgcct tgccgccctc gtcgaccagg ccgccctggt ggccctggtc acgctcgccg
     9721 cgtacaccgt gggcaccgcc gtcatcgagc accgcccgcc cgtccccggg accgtcgccg
     9781 tcctgatcgg gctcgtcctc ctccgggcac tggccacctg gcgcgagatg gacctctcgc
     9841 acgacctcgc ctaccgggtc ctggccgaac tgcgcgtgag ggtcttcgac ggtctggccc
     9901 gcagcgcccc cgcccggatc gcgggccggc gcagcggcga cctcgcggcc accgcgctcg
     9961 gggacgtcga ggcgctggag ttcttctacg cccacgccat cgcccaactc ctcgcctccg
    10021 gcgtggtctt cacggtgtcg gcggccctgc tcgcggccct cgggccctgg ctgctgctcg
    10081 ccgtcgtacc cgccgcgctg ctcctgatct ggtcgccgct gatcgaggcg cgcggacggg
    10141 ccgaacgcgg acaccgcacc cggagcgcgc tcgccgagct gtcgtcggag acggtggaga
    10201 gcgtcgacgg actgcgcgag ctcctgatga cggggtcgct caaccgcagg cgggcccggc
    10261 tccggtccgc cgggcggcgg ctcgcccgcg cccagcgcgc cgaacagtcg tgggagaccg
    10321 gtgcgggcgc cgcccgcgac ctgctggtcg tcgccgccgt catcggggtc gtggcggccg
    10381 ccgccgacgc cgcgtccgcc ggacgcctcg acggggcatg ggccccggcc gccatggcgc
    10441 tcgccctggg cgccctggcc ccggtcgcgg aatcgacggc cgcgctcggc agggcgggtg
    10501 gcctgcgcgc cgccgcggcc cgggtccggg ccgccgtgca cgccccggcg ggagcgcccg
    10561 ccgccaccga cccccgcccc gtaccgccgg gcccgctcgg tctgcggctg cgcggcgtac
    10621 ggttcggcta cggcggagcg gccgtgctgg acggcgtcga cctcaccgtc cggccggggg
    10681 agaccgtggc cctcgtcgga gcgtccgggg ccggcaagtc gacctgcgcc cacctcctgg
    10741 cccgctactg ggacccggcc gaaggcgccg tggaactcgt gccccccggc ccggcccacc
    10801 ccgtcgacct gcgggacctc gccgaggacg acctgcgggc cgccgtgtcc gtcgtggggc
    10861 aggaggcccc gctcttccac ggcacgctcg ccgagaacct ccgcctcggc gtccccgacg
    10921 cgacggcgga cgagctggac gcggtggcgc ggatctgcgg cgtcgacacc gtcgccgaca
    10981 ccctgcccga cggtctcgac accgccgtcg gcgagcgcgg cgcgaccctc tccggcggcc
    11041 agcgcgcccg ggtggccctg gcccggggtc tgctgagcgg cccgcgcgtc ctcgtcctgg
    11101 acgagacgac cgcccatctg gaccaccggg gcgacgcgga actggcccgg gcactcgcgg
    11161 ccgactccgc cacccgggcg accctggtga tcgcccaccg cccggccacc gtccgcaggg
    11221 ccgaccggat cgccgtcctg gacggcgggc gcatcgtcga ggaaggcacc tgggacgagc
    11281 tgatccgggc cgacggtccc ctgacccgcc tcttcgcccg cgaccggtcg ccggacgcgg
    11341 gggtcaggga atcggctgct ccgcccag
//
