LOCUS       NZ_KZ679082            11335 bp    DNA     linear   CON 17-JAN-2024
DEFINITION  Streptomyces sp. CS149 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ679082
VERSION     NZ_KZ679082.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS149
  ORGANISM  Streptomyces sp. CS149
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7754255)
  AUTHORS   Malmierca,M.G., Perez-Victoria,I., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Cooperative Involvement of Glycosyltransferases in the Transfer of
            Amino Sugars during the Biosynthesis of the Macrolactam Sipanmycin
            by Streptomyces sp. Strain CS149
  JOURNAL   Appl Environ Microbiol 84 (18), e01462-18 (2018)
   PUBMED   30006405
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754255)
  AUTHORS   Olano,C., Malmierca,M.G., Mendez,C. and Salas,J.A.
  TITLE     Characterization of the biosynthesis gene cluster for the
            macrolactam sipanmycins: involvement of four glycosyltransferases in
            the transfer of two aminosugars
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7754255)
  AUTHORS   Olano,C., Malmierca,M.G., Mendez,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2018) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 556.87x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/17/2024 11:43:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,915
            CDSs (total)                      :: 6,834
            Genes (coding)                    :: 6,691
            CDSs (with protein)               :: 6,691
            Genes (RNA)                       :: 81
            rRNAs                             :: 12, 7 (16S, 23S)
            complete rRNAs                    :: 12, 7 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 52 of 144
            Pseudo Genes (incomplete)         :: 108 of 144
            Pseudo Genes (internal stop)      :: 12 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:40:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6500195
            Orig. end                         :: 6511530
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ679082.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Professor JA Salas
            laboratory.
FEATURES             Location/Qualifiers
     region          1..11335
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="24"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11335
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11335
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6335](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6335)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(887..903)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.3"
                     /tool="antismash"
     gene            1314..2042
                     /gene="hemQ"
                     /locus_tag="C6W96_RS29165"
                     /old_locus_tag="C6W96_29160"
     CDS             1314..2042
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="C6W96_RS29165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_29160"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_107413895.1"
                     /transl_table=11
                     /translation="MSAPETVTSSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRA
                     GYADEVQELFDQLAAKDITVRGTYDLSGLRADADIMIWWHAETSDQLQDAYNLFRRTKL
                     GRALDPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARLHVRE
                     EVPFYTGRRKSVADLVVGLA"
     gene            complement(2202..3764)
                     /locus_tag="C6W96_RS29170"
                     /old_locus_tag="C6W96_29165"
     CDS             complement(2202..3764)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (101-476). E-value:
                     1.9e-19. Score: 62.2. Matches aSDomain:
                     nrpspksdomains_C6W96_RS29170_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="C6W96_RS29170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_29165"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_107413896.1"
                     /sec_met_domain="PF00561 (E-value: 4e-25, bitscore: 87.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAALYTSIGSLVLSALAAAPAGAWDRPGSAEARGTATAAARAT
                     AAGIDFVACPEEEMLPDSLKCGTVKVPLDYADPDGRQLELTVSRTPATGPAKERQGAFV
                     YNPGGPGASSITFPLAGELPEWKEIAEAYDLVGYAPRGVNGSSAPLSCQDPSAYTKGPT
                     DAPTHPTEADKERRVARAKAYARGCAEHAGETLRHYTSLNNARDLDVLRAALGESRLTF
                     MGASYGTYFGALYATLFPSHVRRMVFDSAVDPDPEQIWYRSNMGQSLAFEARWEDFRRW
                     VAKHDDVYHLGTTPEAVQERYDEVRAELAVEPAGGKVGPGQLHSAFLGAGYYDDYWAMR
                     ATALSEYVRGNPEPLIAQASPRAVAAKDNENAQAVYTAVECNDAPWPEDFEVWDRDHTE
                     LARVAPFETWDNAFSNLPCAFWPAPQQRPLDVRVGWGELPPVLILAAERDAATPYKGAL
                     ELRKRLPGSSLVTERDAGTHGIGGAGNACVDDHLRRYLLTGEVPGRGANCAAHPEPNPV
                     SLD"
     aSDomain        complement(2337..3461)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C6W96_RS29170_Abhydrolase_1.1"
                     /evalue="1.90E-19"
                     /label="C6W96_RS29170_Abhydrolase_1.1"
                     /locus_tag="C6W96_RS29170"
                     /protein_end="476"
                     /protein_start="101"
                     /score="62.2"
                     /tool="antismash"
                     /translation="AFVYNPGGPGASSITFPLAGELPEWKEIAEAYDLVGYAPRGVNGS
                     SAPLSCQDPSAYTKGPTDAPTHPTEADKERRVARAKAYARGCAEHAGETLRHYTSLNNA
                     RDLDVLRAALGESRLTFMGASYGTYFGALYATLFPSHVRRMVFDSAVDPDPEQIWYRSN
                     MGQSLAFEARWEDFRRWVAKHDDVYHLGTTPEAVQERYDEVRAELAVEPAGGKVGPGQL
                     HSAFLGAGYYDDYWAMRATALSEYVRGNPEPLIAQASPRAVAAKDNENAQAVYTAVECN
                     DAPWPEDFEVWDRDHTELARVAPFETWDNAFSNLPCAFWPAPQQRPLDVRVGWGELPPV
                     LILAAERDAATPYKGALELRKRLPGSSLVTERDAG"
     gene            complement(3940..4995)
                     /locus_tag="C6W96_RS29175"
                     /old_locus_tag="C6W96_29170"
     CDS             complement(3940..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072528.1"
                     /locus_tag="C6W96_RS29175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_29170"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_107415099.1"
                     /transl_table=11
                     /translation="MNTLALTATLAVVVSSVLLVAKAVAERSRRPSPGPGVALHDLYEV
                     AFLNGGPARVVDTALTALHADGRLAVGGPGIVAVQRAQANDPVERAVFEALAAAPNGAL
                     HILREAVMRHPAVQEVGDGLAARGLLVPPHRLRPLRRWGLTQGTVCLFALPLSLLLTFV
                     QFVTDDSLDFSVPFIMKMLPAIVIGSFTGLIVANSSAGRLTASGRHAAQSFRAAYAHVV
                     TPAHLVATLGLRALPDPVFQAQLMAAARLSAPRRRSSGRRLAGSTATGVTAVGVATVWC
                     AASGPGGSSCGGSTGGGSGSSCGGGAGCSSGSGCGGSSGSSCGGGSGCGSSSGSSCGGG
                     GGGSSCGGGGS"
     gene            complement(5001..6335)
                     /locus_tag="C6W96_RS29180"
                     /old_locus_tag="C6W96_29175"
     CDS             complement(5001..6335)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072529.1"
                     /locus_tag="C6W96_RS29180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_29175"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_107413897.1"
                     /sec_met_domain="DUF692 (E-value: 2.4e-92, bitscore: 307.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELGIGIGWRPEIAAEVEALSGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVALGLGGADRPDPDRLTALAARAELLGAPLVTEHIAFVRAGGPLTASP
                     RLEAGHLLPVPRTWDALDVLCENVRIAQDSLPVPLALENIAALITWPDEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNLGQDPAKALDELPVEAIAYVHVAGGVEKNGVWHDTHA
                     HPVSEPVLDVLAELRSRVDPPGVLLERDDAFPPGAELAGELDAIRATLRKAAPSAGPGP
                     DRGTAPAPDGTAGTEARTAAPAPAPVSAATRDRTAVAQTALLSALVAGTPAPEGFDHRR
                     LRVQSRALAAKRADVVAKVAPELPEILGDGYRAAFLAYAGSRPMSGGYRRDALDFAEHV
                     LIAGGPADPAARRRLTYWWQDRSGSRPPRRATRLVRAARAVLVGK"
     gene            complement(6457..7203)
                     /locus_tag="C6W96_RS29185"
                     /old_locus_tag="C6W96_29180"
     CDS             complement(6457..7203)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072530.1"
                     /locus_tag="C6W96_RS29185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_29180"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_187452026.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATLGALAFPVWSYADRSGTGEANLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSEATKAAGDHLVVGHTDLDRRAR
                     DVAAKLGVELPNQPTAQQQEWLRELTAASGDEYERKFANILRAAHGKVFGLIGQVRHTT
                     RNTLIRQLASDANQTVLDHITMLEATGLVDFDALAREAASGSTASPSGPSMPRDGQAPL
                     APVPATPTGDQSFTSRPVPPTVMPMP"
     gene            complement(7406..8134)
                     /locus_tag="C6W96_RS29190"
                     /old_locus_tag="C6W96_29185"
     CDS             complement(7406..8134)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072531.1"
                     /locus_tag="C6W96_RS29190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_29185"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_107413899.1"
                     /transl_table=11
                     /translation="MTSHDTPSPASGSPFHSGPTDRDQAPQFVLPLVLHLEKTDPPART
                     DALRAAARAVLTILSDERSLGDGEWAAAMRDWQDARIRKVVRRARGAEWRKAAALDGIT
                     VTGEDGAEVRVFPPIPLDGWPKELAKLQVSGTDLDDPEPPAAPDLSGPVLWLNPDVDMS
                     AGKAMAQAGHGAQLAWWELSGTERKAWREAGFPLSVATPGAERWRELTASGLPVVRDAG
                     FTEIAPGSCTVIADHPALRR"
     gene            8206..8880
                     /locus_tag="C6W96_RS29195"
                     /old_locus_tag="C6W96_29190"
     CDS             8206..8880
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="C6W96_RS29195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_29190"
                     /product="AIM24 family protein"
                     /protein_id="WP_093813298.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHMAQPATAPGMTLQNAKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLALMAVRGQGEAWFAHEAANCFIVELDQGDAIT
                     INGRNVLCFDPTLSYEIKTVKGAGMVGGGLFNSVFTGYGKLGLMCEGTPIVIPVTAAQP
                     VYVDTDAVVGWSQQLTTSLNRSQSVGSMVRGGSGEAVQLMLQGEGFVIVRPSEARPEKT
                     SN"
     gene            8883..9797
                     /locus_tag="C6W96_RS29200"
                     /old_locus_tag="C6W96_29195"
     CDS             8883..9797
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536943.1"
                     /locus_tag="C6W96_RS29200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_29195"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_199854560.1"
                     /transl_table=11
                     /translation="MHLRGVGHRHNPAGRWVLRGIDLALPARALVRVEGGNGAGKSTLL
                     RLLAGIEAPAEGRITGRPARTAYVPERFPAALPLTATGYLVHLGRIHGLRTAEAKRRAG
                     EWLTRFGAAEHARTPLAELSKGTSQKVAVAQALLAAPDLLILDEAWTGLDTAARAELDR
                     AVTERVAADATVVFVDHDPRRLAGAVDLSLRVRDGGVHRATASPATGPRTLIEAEGPPG
                     APLPQDLPGDPLRERGTGTAPDLVRLTVPTPYSDALLTALLTARPPWHIRQVAPVDAPE
                     RPGPPGPPEPIRPSEQPEVSEAP"
     misc_feature    9015..9017
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9794..10462
                     /locus_tag="C6W96_RS29205"
                     /old_locus_tag="C6W96_29200"
     CDS             9794..10462
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452202.1"
                     /locus_tag="C6W96_RS29205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_29200"
                     /product="ABC transporter"
                     /protein_id="WP_107413900.1"
                     /transl_table=11
                     /translation="MTALLRYQAALLARSQRWLAPLLLYVAVLAVGVQSGQPVLGSLGF
                     AAAALLPVTAWAVHLCVGQEPPAARAVASAAAGPRRVHLAALLTATGGAAVLAGVTTAV
                     VLLVSSPATDNGTQAVPRPAAALAGLLTALCCVLLGAVVGALSTRPVLRRRGWSVAAAV
                     LGSLLALVTTGSPARYAITALVTGSRTGTFHPPVLASLGAAALAGTAAFVVCRLAARRG
                     "
     gene            10471..10668
                     /locus_tag="C6W96_RS29210"
                     /old_locus_tag="C6W96_29205"
     CDS             10471..10668
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128422.1"
                     /locus_tag="C6W96_RS29210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_29205"
                     /product="hypothetical protein"
                     /protein_id="WP_199854467.1"
                     /transl_table=11
                     /translation="MDVDDTYCEPPGRIEPAPPAGTAEGPPYAECVLCREPTEYPESTK
                     GAMLCPVCAWQEAGRTACSG"
     misc_feature    10901..10914
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.73"
                     /tool="antismash"
     misc_feature    10985..10999
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.29"
                     /tool="antismash"
ORIGIN
        1 gaccggcgag atcgcgggcg tccaggtcga cctgggggcc gagtcgatgc tcgcccggcg
       61 tcccgaggcg gtcgcgctgg ccgaggcggt gggcctcggc gaccggctcc agccgcccgc
      121 caccgccacc gcgtcgctct ggacgcgcga cgcgctgcgc ccgatgccca agggccacgt
      181 catgggcgtc cccggcgacc cggcggtcct cggcgaggtg ctgtcccccg agggactggc
      241 ccggatcgcc gaggagcgcg acctcacccc gacccccgtc ggcgacgacg tggcggtcgg
      301 cgcgtacgtg tccgaccggc tcggccgcga ggtcgtcgac cggctggtgg agcccctcct
      361 cggcggggtc tacgcgggcg acgcctaccg gatctcgatg cgcgccgccg taccccagct
      421 cttcgaggcc gtgaaggagg gcggcccgct gctcgacggc gtccgccgca tccaggagcg
      481 ggccgccgcc cggcagcaga ccggccccgt cttccagggc atcgcgggcg gcatcggcgc
      541 cctcccggcc gccgtcggcg acgccgtacg ggcccagggc ggcgagatcc tcaccgagac
      601 ccccgtcctc ggcctgaccc gtacggaaac gggctgggcc gtccgcaccg acacccggac
      661 catcaccgcc gacggcatcg tcctggccac ccccgcctgg tcggccggca ccctgctggc
      721 cgccgagtcc ccggccgtct ccgccgagct gtccggcgtc gagtacgcgt cgatggccct
      781 ggtcaccctg gccttccggc gcaccgacat cgaggcgtac gaggccctgc acggccgctc
      841 cggcttcctg gtgccgccgg tcgacgggca cacgatcaag gcgtccacgt tctccagcaa
      901 caagtggcag tgggtcgccg acgccgcccc cgacctgttc gtgctccgca cctccgtcgg
      961 ccgctacggc gaggaggacc acctccaccg cgaggacgag gagctcgtcg ccgtctccct
     1021 gcgcgacctc gccgccgcca ccggcctcac cgcccggccc gttgacaccg aggtcacccg
     1081 ctggatcggc ggcctgccgc agtacccggt cggccacctc acccgggtcg cccgcatccg
     1141 cgacgaggtc gccaagctgc ccgccctgcg ggtctgcggc gcggtctacg acggggtggg
     1201 catccccgcc tgcatcgcga gcgcgcaccg cgcggccgac gagatcgcga gcgagatcat
     1261 cgccacgccg accctggttc ggggcacggg gagcgaggcg ggacaatagc cgtatgagtg
     1321 ctcctgagac tgtgacatca agcaagagtc cgaacgccgg caagaaggcc aaggacctca
     1381 acgaggtcat ccgctacacc ctgtggtcgg tcttcaaact ccgcgacgtc ctccccctgg
     1441 accgcgccgg atacgccgac gaggtgcagg agctgttcga ccagctcgcg gccaaggaca
     1501 tcaccgtccg gggcacctac gacctgtccg ggctccgggc cgacgcggac atcatgatct
     1561 ggtggcacgc cgagacctcg gaccagctcc aggacgcgta caacctcttc aggcgcacca
     1621 agctcggccg cgcgctcgac ccggtctggt cgaacatggc gctgcaccgc cccgccgagt
     1681 tcaacaagtc gcacatcccg gcgttcctcg ccgacgagac gccccgcgac tacatcagcg
     1741 tctacccctt cgtccggtcc tacgactggt acctgctgcc cgacgaggac cgccgccgca
     1801 tgctcgcgga ccacggcaag atggcccggg gctacccgga cgtccgcgcc aacaccgtcg
     1861 cctcgttctc gctcggcgac tacgagtgga tcctcgcctt cgaggcggac gagctgtacc
     1921 gcatcgtcga cctgatgcgt cacctgcgcg cctccgaggc acggctccac gtgcgcgagg
     1981 aggtcccgtt ctacacgggc cgcaggaaga gcgtcgcaga cctggtggtc gggctcgcat
     2041 aaccagcgag tcccgcacca ccccaacccg gaagatcaga gagtcgggca ccgggcaccg
     2101 gtcgtgccgt ccgcctctcc agcgacaccg ggttcggctt cgggggccgt cgcaccatca
     2161 cggtgcggcg gcccccgctc cgtacgctgc cgaccgccgg atcagtccag cgacaccggg
     2221 ttgggctccg gatgcgcggc gcagtttgca ccccgccccg gcacctcacc ggtcagcaga
     2281 taccggcgca gatgatcgtc gacgcaggcg ttccccgccc cgccgatgcc atgcgtcccc
     2341 gcgtcccgct cggtcaccag cgacgaaccg ggcagccgct tccgcagctc cagcgccccc
     2401 ttgtacgggg tcgccgcgtc ccgctccgcc gccaggatca gcaccggcgg cagctcgccc
     2461 caccccacgc ggacgtccag cggccgctgc tggggagcgg gccagaaggc gcacggcagg
     2521 ttcgagaagg cgttgtccca ggtctcgaag ggggccacgc gcgccagctc cgtgtggtcg
     2581 cggtcccaga cctcgaagtc ctcgggccag ggcgcgtcgt tgcactcgac ggccgtgtac
     2641 accgcctggg cgttctcgtt gtccttcgcc gccaccgcgc gcggggacgc ctgcgcgatc
     2701 aacggctccg ggttgccccg gacgtactcc gacagcgccg tcgcccgcat cgcccagtag
     2761 tcgtcgtagt aacccgcccc caggaacgcc gagtgcagct gccccggccc gaccttgccg
     2821 cccgcgggct ccaccgccag ctcggcgcgc acctcgtcgt agcgctcctg caccgcttcc
     2881 ggcgtggtcc ccaggtggta cacgtcgtcg tgcttggcga cccagcgccg gaagtcctcc
     2941 caccgcgctt caaaggccag cgactgaccc atgttggagc ggtaccagat ctgctccggg
     3001 tccgggtcga ccgccgagtc gaagaccatg cgccgtacgt gggaggggaa cagcgtcgcg
     3061 tacagcgccc cgaagtacgt cccgtacgac gcccccatga acgtcagccg gctctcgccg
     3121 agcgcggccc gcagcacgtc caggtcgcgg gcgttgttga gcgaggtgta gtggcgcagc
     3181 gtctcgcccg cgtgctccgc gcagccccgc gcgtacgcct tcgcccgggc gacgcgccgt
     3241 tccttgtcgg cctcggtcgg atgcgtcggt gcgtcggtcg ggcccttcgt gtacgcggaa
     3301 gggtcctggc aactcagcgg ggcggaggag ccgttcaccc cacgcggggc gtagccgacc
     3361 aggtcgtacg cctcggcgat ctccttccac tccggcagct ccccggccag cgggaaggtg
     3421 atgctggacg cgccggggcc gcccgggttg tagacgaacg cgccctgccg ctccttcgcc
     3481 ggaccggtgg cgggcgtgcg gctgacggtc agttcgagct gccgcccgtc cggatccgcg
     3541 tagtcgaggg gcaccttcac cgtgccgcac ttcagggagt ccgggagcat ctcctcctcg
     3601 gggcacgcga cgaagtcgat cccggcggcc gtggcccggg cggcggcggt cgcggtgccc
     3661 cgggcctcgg cggaaccggg gcggtcccag gcccccgccg gagcggcggc gagggcggac
     3721 aggaccaggg agccgatcga tgtgtagagc gcagcagttc tcacgcgcgg gggcctctct
     3781 gcggatgcgg agagggatgg tcgggcggcg gggcggacat gtccagtacc gccccggaat
     3841 gtcaccgtaa tccaccctga tggacgctca agaaccgtac gtacgcgccc gattcaggtc
     3901 gcagaagacc gattcagaag accgattcag gagaccggtt caggagcccc cgcccccaca
     3961 gctggatccg ccgcccccgc ccccacagct ggagccactg gagctgccgc aaccggagcc
     4021 gcctccgcag ctcgacccgc tgctaccacc gcacccggat ccggaggagc acccggcccc
     4081 gccgccgcag ctcgatccgg agccgccccc ggtcgacccg ccgcaactgg agccgccggg
     4141 gccggacgcg gcgcaccaga ccgtcgccac gccgacggcg gtgacacccg tcgccgtgga
     4201 cccggcgagg cgccgcccgg aggaccggcg gcgcggcgcg ctcagccgtg ccgcggccat
     4261 caactgggcc tggaacaccg ggtccggcag ggcgcgcagg cccagggtgg cgacgagatg
     4321 ggcgggggtc accacgtggg cgtaggcggc ccggaacgac tgggcggcgt ggcgccccga
     4381 cgccgtgagg cggcccgcgg aggagttggc gacgatcagc ccggtgaacg agccgatcac
     4441 gatcgcgggc agcatcttca tgatgaacgg gaccgagaag tccagggagt cgtccgtgac
     4501 gaactggacg aacgtcagca gcaggctcag cggcagggcg aacaggcaca cggtcccctg
     4561 ggtcaggccc caccgccgca gcgggcggag ccggtggggc ggcaccagca gcccgcgcgc
     4621 ggcgagcccg tcgccgacct cctggaccgc cgggtgccgc atcaccgctt cccgcaggat
     4681 gtgcagggcg ccgttcgggg cggcggccag cgcctcgaac accgcgcgct ccaccgggtc
     4741 gttcgcctgc gcccgctgta cggcgacgat gccgggcccg cccacggcca gccggccgtc
     4801 cgcgtgcagg gcggtcaggg cggtgtccac gaccctggcc ggaccaccgt tcaggaacgc
     4861 gacctcgtac aggtcgtgaa gggcgacgcc gggacctggg gaggggcggc gggaccgctc
     4921 ggccaccgct ttggcgacga gcagcaccga cgagacgacg accgcgaggg tggccgtcag
     4981 agcgagcgtg ttcacggtgg tcacttcccc acgaggacgg cacgggccgc ccggaccaga
     5041 cgggtggcac ggcgcggggg ccgggagccc gaccggtcct gccaccagta cgtcagccgg
     5101 cgccgcgccg ccggatcggc gggaccgccc gcgatgagca cgtgctcggc gaagtccagg
     5161 gcgtcgcgac ggtagccgcc ggacatgggg cggctcccgg cgtatgcgag gaacgcggcg
     5221 cggtagccgt cgccgaggat ctccggcagc tcgggggcca ccttggcgac gacgtcggcg
     5281 cgcttcgcgg ccagcgcccg gctctgcacc cgcagccgcc ggtggtcgaa gccctcgggg
     5341 gccggggttc cggcgaccag ggcggagagc agtgcggtct gtgcgacggc ggtacggtca
     5401 cgggtggcgg cggacacggg tgcgggtgcg ggtgcggccg tacgggcttc ggtgcctgcg
     5461 gtgccgtcgg gcgcgggagc ggtaccccgg tccggccccg ggcccgccga gggtgccgcc
     5521 ttccgcagcg tcgcccggat cgcgtccagt tcgcccgcca gctccgcgcc cggcgggaag
     5581 gcgtcgtccc gctccagcag cacgcccggc ggatcgaccc gggagcgcag ctcggccagc
     5641 acgtccagta cgggttcgga caccgggtgg gcgtgggtgt cgtgccagac gccgttcttc
     5701 tcgaccccgc ccgccacatg gacgtacgcg atggcctcca ccggcagttc gtccagcgcc
     5761 ttcgccgggt cctggccgag gttgacgtgg ttggtgtgca ggttggccac gtcgatcagc
     5821 agtcgcaccc cggtccgctc gaccagctcc gccaggaact gcccctcggt gagctcctcg
     5881 tcgggccagg tgatcagcgc ggcgatgttc tccagggcga gcggcaccgg cagcgagtcc
     5941 tgcgcgatcc gcacgttctc gcagagcacg tccagcgcgt cccaggtgcg gggcaccggc
     6001 agcaggtgcc ccgcctccag cctcggcgag gcggtcagcg gccccccggc ccgtacgaac
     6061 gcgatgtgct ccgtcaccag gggcgccccc agcagttcgg cgcgcgccgc gagagcggtg
     6121 agccggtcgg ggtcgggccg gtccgcgccg ccgaggccga gcgccacccc gtgcggcacg
     6181 acggtcaccc cgcgttcccg gagccgtacg agggagtcgg ggaggtggtc ggcgcagagg
     6241 ttctccgcga ccgcctccac ccagtcgatc ccgctcagcg cctccacctc ggccgcgatc
     6301 tccggccgcc agccgatacc gatgcccagt tccatggtgc ccccgtcccc ctgctcacct
     6361 cgttctgttc aggggtcatg gccccgtcgc gcggcgatga acccgaccgg tgggacgttc
     6421 agagcttcat ttgaggttcc ggacccgccg ccgtcgtcac ggcatcggca tcaccgtggg
     6481 cggcaccggg cgtgaggtga aggactggtc tccggtcggt gtcgccggca ccggggccag
     6541 cggggcctgg ccgtcgcggg gcatcgacgg accggaagga ctggccgtcg atccgctcgc
     6601 cgcttcgcgc gccagggcgt cgaagtcgac gagcccggtg gcctccagca tggtgatgtg
     6661 gtccagcacg gtctggttgg cgtcgctcgc cagctgccgg atcagcgtgt tccgggtggt
     6721 gtggcggacc tggccgatca ggccgaagac cttgccgtgg gcggcgcgca ggatattggc
     6781 gaactttcgc tcgtactcgt ccccgctcgc cgccgtcagc tcccgcagcc actcctgctg
     6841 ctgggcggtc ggctggttgg gcagctccac cccgagcttc gccgcgacgt cccgggcccg
     6901 ccggtccagg tccgtgtgcc cgacgacgag gtggtcgccg gcggccttcg tcgcctcgct
     6961 gggcgcccgt tcgatcgcct gctgcccggc gggcagctcc cacagcccgg cgagccgcac
     7021 cttgaccagg aagtcccggt cggtggcgga gagcggcccc cactgcgtgg cgacactgga
     7081 ggcgttgagg ttcgcctcgc cggtgcccga acggtcggcg taggaccaga cggggaaggc
     7141 gagtgcgccg agtgtggcga ccagtgccgc gatgatgagg gccgtgccat tgatgcgtcg
     7201 caacaaggtg tctcccgggc cgaggtgtga cagcgtcgat cgaacaggcg tcaatcaacc
     7261 gccaatcgac tggctgtgca ggctacttgg cggtaatgcc gatagggctg taccgggaga
     7321 tacgtaagag gaacgagaaa cgttcagcgc gcgggcgatg gggtcgcggc tccgcgacga
     7381 gggctcgctg tggccgccgt cccgttcagc ggcgcagcgc cgggtggtcg gcgatcacgg
     7441 tgcaggagcc cggggcgatc tcggtgaacc ccgcgtcgcg caccaccggc agcccgctcg
     7501 ccgtcagctc ccgccagcgc tccgccccgg gggtggccac ggagagcggg aacccggcct
     7561 cgcgccaggc cttgcgctcg gtgccggaca gctcccacca ggcgagctga gccccgtgcc
     7621 cggcctgggc catcgccttg cccgccgaca tgtcgacatc ggggttcagc cacaggaccg
     7681 ggcccgagag gtcgggcgcg gcgggcggct cggggtcgtc caggtccgtg cccgacacct
     7741 ggagcttggc cagctccttg ggccagccgt ccagcgggat cggcgggaac acccgcacct
     7801 cggcgccgtc ctcgccggtc accgtgatgc cgtcgagcgc cgccgccttg cgccactccg
     7861 caccgcgcgc ccggcgcacg accttccgga tccgggcgtc ctgccagtcc cgcatggccg
     7921 ccgcccactc gccgtcgccg agggaccgct cgtcggagag gatcgtcagc accgcccggg
     7981 cggcggcccg cagtgcgtcg gtgcgggcgg gcgggtccgt cttctccagg tggagcacca
     8041 ggggcagcac gaactgcggt gcctggtcgc ggtcggtcgg gccgctgtgg aacgggctcc
     8101 cggaggcggg ggagggtgtg tcatggctgg tcaccgtccc agtctgccag gcggacgagg
     8161 tcacctgctt ggcggaacga ccgggtccgg gtgaggatgc tccgcatgaa gagcgatctc
     8221 ttttccagcg agcacatggc ccagccggcc accgccccgg gtatgaccct gcagaacgcg
     8281 aaatccatca agtacgccgt caacggggag atgcacgccc ggcagggctc gatgatcgcc
     8341 ttccgcggca acctccagtt cgagcgcaag ggccagggca tcggcggcat gctcaagcgc
     8401 gccgtcaccg gcgagggcct cgcactgatg gccgtccggg gccagggcga ggcctggttc
     8461 gcgcacgagg cggcgaactg tttcatcgtg gagctggacc agggcgacgc catcaccatc
     8521 aacggccgca acgtcctctg cttcgacccc acgctctcct acgagatcaa gaccgtgaag
     8581 ggcgccggaa tggtcggcgg cggcctcttc aacagcgtct tcacgggcta tggcaagctc
     8641 ggcctgatgt gcgagggcac cccgatcgtg atccccgtga cggccgccca gccggtgtac
     8701 gtggacacgg acgcggtcgt cggctggagc cagcagctca cgaccagcct gaaccgctcc
     8761 cagagcgtcg gctcgatggt ccgcggcggc tccggcgagg ccgtccagct gatgctccag
     8821 ggcgagggct tcgtcatcgt ccgcccgagc gaggccaggc ccgagaagac ctccaactga
     8881 gcctgcacct gaggggcgtg ggccaccgcc acaaccccgc cggccgctgg gtgctgcgcg
     8941 ggatcgacct cgccctgccc gcacgggccc tcgtccgcgt cgagggcggc aacggcgcgg
     9001 gcaagtcgac cctgttacgg ctgctcgccg ggatcgaggc ccccgccgag gggcggatca
     9061 ccggccgccc ggcccgtacg gcgtacgtcc ccgagcgctt cccggccgcc ctgcccctga
     9121 ccgccaccgg ctacctcgtc cacctcggcc ggatccacgg actgcggacc gccgaggcga
     9181 agcggcgggc gggggagtgg ctgacccggt tcggggcggc ggagcacgcc cgtaccccgc
     9241 tcgccgagct ctccaagggc accagccaga aggtcgccgt agctcaggcg ttgctggcag
     9301 ccccggacct gctgatcctg gacgaggcct ggacggggct cgacacggcg gcccgagccg
     9361 aactcgaccg tgccgtgacc gaacgcgtcg cggccgacgc caccgtggtc ttcgtcgacc
     9421 acgacccgcg acggctcgcc ggggcggtgg acctctccct gcgggttagg gacggtgggg
     9481 tccaccgggc gacagcttcg cctgcgaccg gcccccgcac cctgatcgag gccgagggcc
     9541 cgcccggcgc gccgctgccg caggacctcc cgggcgaccc actccgcgaa cgagggacgg
     9601 gaacggcccc cgacctcgta cgcctcaccg tcccgacgcc gtactccgac gccctgctga
     9661 cggccttgct gacggcccgt ccgccgtggc acatccggca ggtggcgcct gtcgacgcac
     9721 ccgagcggcc tggtccgcct ggtccacctg agccgatacg gccgtccgaa cagcccgagg
     9781 tgtccgaagc gccatgaccg ccctcctccg ctaccaggcc gccctgctcg cccgctccca
     9841 gcgctggctg gcgcccctcc tgctgtacgt ggccgtcctc gccgtcggcg tgcagagcgg
     9901 gcagcccgtg ctgggttcgc tcggtttcgc ggccgccgcc ctgctgcccg tcaccgcgtg
     9961 ggccgtccac ctctgcgtcg gccaggagcc gcccgccgca cgggcggtcg cctcggccgc
    10021 cgccggaccc cgacgggtcc acctggccgc gctgctcacc gcgaccggcg gtgcggcggt
    10081 gctcgccgga gtgaccaccg cggtcgtcct gctggtcagc agcccggcca ccgacaacgg
    10141 cacccaggcc gtcccccgcc cggccgccgc gctcgccgga ctgctcacgg ccctgtgctg
    10201 cgtgctgctg ggcgcggtcg tgggggcgct cagcacccgc ccggtgctgc gccgccgggg
    10261 ctggtccgtc gcggcggccg tgctcggctc gctcctcgcg ctcgtgacca ccggctcccc
    10321 ggcgcggtac gcgatcaccg ccctggtcac cggatcgcgg accgggacgt tccacccgcc
    10381 cgtcctggcg tcgctcgggg cggcggcgct cgccgggaca gcggcgttcg tcgtctgccg
    10441 gctcgccgcc cggcgcggct gagtaggctc gtggacgtgg acgacaccta ctgcgagccc
    10501 cctgggcgga tcgagcctgc cccgcccgcc ggaaccgccg aaggccctcc gtacgccgaa
    10561 tgcgtgctgt gccgcgagcc gaccgaatac ccggagtcga cgaagggcgc catgctctgc
    10621 ccggtctgtg cctggcagga ggcgggccgg acggcctgtt ccggctaggt actgtccggc
    10681 cgatcatgtg cctgtggtgg aacacgcatg atgtcgccca ggtcctggca tctgcgacgc
    10741 caccacggcc aggcactggc tgaacagggc gtaacggtgc tgttcaaggc cgacgctgag
    10801 cggatggcag ggaggggcaa gccttggacc ttcgtcgccg gtggagcgcc ggtgcgcggg
    10861 aacgtcctcg tgcgaaccga tgccacgtct gtggggcaaa gccactcagc gtgcttgccc
    10921 cgcctgcgtg agctgggcct ttcgttccct gagtgagact tcaggtgtga gcgaaccgcc
    10981 gccctggatg tcagtgccag ctgcttgcat gagctcatgg acaccgtgca cacgactgcc
    11041 gccctcctcc cggaccccgc cgggctccgc acgtacctgc gtgcgctggc cgtgctggac
    11101 caggcgatct gccgcgaccc gcggttctct cgctactcct tctccaccac ctgggggcct
    11161 gggacggagg cggccctgat ggacaacggc tcgggtgacg acttctccgt cctcttcacc
    11221 ccggccggcg tgctcgtccg cggcttcgac cacgagtcgg agatgagccc gtacggcacg
    11281 gatgacgaac aggtctggcc tggtgtcatc gacgaggtgc ccgccgcgct gcgcc
//
