LOCUS       NZ_KZ679082            10888 bp    DNA     linear   CON 17-JAN-2024
DEFINITION  Streptomyces sp. CS149 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ679082
VERSION     NZ_KZ679082.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS149
  ORGANISM  Streptomyces sp. CS149
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7754255)
  AUTHORS   Malmierca,M.G., Perez-Victoria,I., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Cooperative Involvement of Glycosyltransferases in the Transfer of
            Amino Sugars during the Biosynthesis of the Macrolactam Sipanmycin
            by Streptomyces sp. Strain CS149
  JOURNAL   Appl Environ Microbiol 84 (18), e01462-18 (2018)
   PUBMED   30006405
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754255)
  AUTHORS   Olano,C., Malmierca,M.G., Mendez,C. and Salas,J.A.
  TITLE     Characterization of the biosynthesis gene cluster for the
            macrolactam sipanmycins: involvement of four glycosyltransferases in
            the transfer of two aminosugars
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7754255)
  AUTHORS   Olano,C., Malmierca,M.G., Mendez,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2018) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 556.87x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/17/2024 11:43:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,915
            CDSs (total)                      :: 6,834
            Genes (coding)                    :: 6,691
            CDSs (with protein)               :: 6,691
            Genes (RNA)                       :: 81
            rRNAs                             :: 12, 7 (16S, 23S)
            complete rRNAs                    :: 12, 7 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 52 of 144
            Pseudo Genes (incomplete)         :: 108 of 144
            Pseudo Genes (internal stop)      :: 12 of 144
            Pseudo Genes (multiple problems)  :: 27 of 144
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:40:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 42395
            Orig. end                         :: 53283
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ679082.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Professor JA Salas
            laboratory.
FEATURES             Location/Qualifiers
     region          1..10888
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10888
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10888
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5888](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5888)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    187..202
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.58"
                     /tool="antismash"
     misc_feature    complement(1009..1018)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(1131..2171)
                     /locus_tag="C6W96_RS00190"
                     /old_locus_tag="C6W96_00185"
     CDS             complement(1131..2171)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-269). E-value: 4.5e-13.
                     Score: 40.9. Matches aSDomain:
                     nrpspksdomains_C6W96_RS00190_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="C6W96_RS00190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_00185"
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_107409698.1"
                     /sec_met_domain="ADH_N (E-value: 6.5e-28, bitscore: 95.4,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.8e-21, bitscore:
                     75.2, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLERTTIERRPVGEFDILIDIKFAGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVAETGSGVTRFKVGDRVGVGCMVDSCGQCDACLMGR
                     EQHCAEGNTQTYNALDRSGEPTYGGYSTHLVVTEKFAVTIPEGIALDEAAPLLCAGITT
                     YSPLKRWGAGPGKKVAVVGLGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLGLGADHY
                     YATSDPKTFEELAGTFDVILSTVSAPLDFGAYLGLLRTEGTLVNVGAPEEPISLNLFSL
                     ILGNRSIAGSAIGGIKETQEMLDFCAVHGLGAEIEVIDASQVNEAYERVLASDVRYRFV
                     IDTATI"
     aSDomain        complement(1365..2114)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C6W96_RS00190_PKS_ER.1"
                     /evalue="4.50E-13"
                     /label="C6W96_RS00190_PKS_ER.1"
                     /locus_tag="C6W96_RS00190"
                     /protein_end="269"
                     /protein_start="19"
                     /score="40.9"
                     /tool="antismash"
                     /translation="TIERRPVGEFDILIDIKFAGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVAETGSGVTRFKVGDRVGVGCMVDSCGQCDACLMGREQHCAEGNTQTYNALDRSG
                     EPTYGGYSTHLVVTEKFAVTIPEGIALDEAAPLLCAGITTYSPLKRWGAGPGKKVAVVG
                     LGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLGLGADHYYATSDPKTFEELAGTFDVI
                     LSTVSAPLDFGAYLGLLRTEGTLVNVGA"
     gene            2334..3263
                     /locus_tag="C6W96_RS00195"
                     /old_locus_tag="C6W96_00190"
     CDS             2334..3263
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956390.1"
                     /locus_tag="C6W96_RS00195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_00190"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_107409699.1"
                     /transl_table=11
                     /translation="MDDQPEAAAPEPLDRRAELSEFLRTRRAKLQPQDVGLPEFGRARR
                     VPGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLTDAEHAHLNHLARP
                     ARHKKKRRPAKVQRVRTGLLYLLDSMEGIPAYVAGARSDILAWNPMAAAVFGNWAALPA
                     AERNWARLVFLSPAYRDLFLNWDSKASDMVSYLRLYAGRHPDDPQLSALVGELSLKSEE
                     FRRLWATHNVKEKGHGIKLIRHPLVGDLTLSYETLNLPDDEDQQLVTYHAEPGSESAQA
                     LRLLASWGADAGRAHVISGAGRSPDDER"
     gene            complement(3303..4004)
                     /locus_tag="C6W96_RS00200"
                     /old_locus_tag="C6W96_00195"
     CDS             complement(3303..4004)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606639.1"
                     /locus_tag="C6W96_RS00200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_00195"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_107409700.1"
                     /transl_table=11
                     /translation="MTKQERATRTRQALIRSAAVVFEQHGYAQARLTLISSGAGVSTGA
                     LHFHFENKAAVAEAVVAEASRGLRDMSAAIRRGTDTALQALVDTSHALVELLRGDPVSR
                     AGFRLSCDGERAAAPDPRMEWHHRVRELLDEAAAAGTLADDIAREDAAAATVAVTAGFE
                     VLGRHDTEWLSPYRLTGFWQLLLPRLAAAKTLPLLDPAGTGAVASARAPALAASAVGEA
                     EQTAALTAEPT"
     gene            complement(4139..4987)
                     /locus_tag="C6W96_RS00205"
                     /old_locus_tag="C6W96_00200"
     CDS             complement(4139..4987)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060637.1"
                     /locus_tag="C6W96_RS00205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_00200"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_107409701.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGREVAALLAATGPVRILARRPDRVTVRGDGVEVV
                     EGAYGDRPALDRALRGVNSLFLVTNSPTEPDDEHVAEAARAAGVRHLVKLSMMAVEEPG
                     ADDFITRRQRENEEAIRESGIAWTFVRPRTFMSNTLSWAPGIRSAGVVRALYGDAPVAC
                     VDPRDVAAVAVAVLTGTGHEGRAYAVSGPEAITAREQTAQLSRVLGRPLRFEELGLDAA
                     RAALLAKYPRPVAEAFLESAERQRAGAKAAVVPTVQELTGRGARTYRTWSADHADAFAA
                     G"
     gene            complement(5001..5888)
                     /locus_tag="C6W96_RS00210"
                     /old_locus_tag="C6W96_00205"
     CDS             complement(5001..5888)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060639.1"
                     /locus_tag="C6W96_RS00210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_00205"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_316248003.1"
                     /sec_met_domain="AfsA (E-value: 1e-25, bitscore: 88.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVHPVGIDMVHRTRPEDAFPRNWVRLAHDRFSVAAVLPHDHPFFA
                     PVGGDRHDPLLVAEAMRQAAMLAFHAGYGVPLGYHFLMADLDYVCHQDHLGVGGAPTEI
                     DVEVFCSDLKWRSGLPVQGRVGWAVHRGGELAATGMGATRFTSPKVYRRMRGDAPAEGI
                     SIPEAAPVDAARAGRSRTEDVVLSDTGREGVWELRVDTRHPTLFQRPNDHVPGMLLLEA
                     ARQAACLAAGPGGIVPAEASTRFHRYAEFGSPCWISAVIQPEEAEDTVTVRVTGHQDDE
                     PVFSTLLSGPRADG"
     gene            6106..6744
                     /locus_tag="C6W96_RS00215"
                     /old_locus_tag="C6W96_00210"
     CDS             6106..6744
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060641.1"
                     /locus_tag="C6W96_RS00215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_00210"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_107409703.1"
                     /transl_table=11
                     /translation="MAKQDRAIRTRQAILMAAAQAFEKYGYQAATITEILKSADVTKGA
                     LYFHFPSKEDLALGVLHAQEPPQAVPEQSLKLQELIDLGMLFSHRLQTSLLARAGVRLS
                     MDQQAHGLDRSGPFLRWQEATHHLLAQAKHNGELLPHVNPTETADLYVAAFTGTQAVSQ
                     TLTNYQDLQRRHITLQQHILPSIAAPSILTALDLTPARAERLSRLAPED"
     gene            complement(6769..7734)
                     /locus_tag="C6W96_RS00220"
                     /old_locus_tag="C6W96_00215"
     CDS             complement(6769..7734)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060643.1"
                     /locus_tag="C6W96_RS00220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_00215"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_107409704.1"
                     /transl_table=11
                     /translation="MGNVHRVAVLALDGVYPFELGIPNRVLGAADGRYSVVTCTVDGRP
                     VRTNADFSITVEHGPEVLATADTLVIPPFDTSLLTREAPADALAALAAVRPGTRIVSIC
                     TGAFLLASAGLLDGRRATTHWALAELFRSWYPQVRLDPGVLFIDDGGVLTSAGAASGVD
                     VCLHLVRADHGSEIANRVARLCVVPPWRDGGQAQYIEQPVPAPAANGTAATRDWALRNL
                     QEPLSLASLAEHARMSRRTFVRRFNEEIGMSPGRWIVQQRVDRARHLLESTDLAVDEIA
                     GQVGFATGASLRQHLHAAIGVTPLAYRRTFRGAVFAAGRP"
     gene            7804..8754
                     /locus_tag="C6W96_RS00225"
                     /old_locus_tag="C6W96_00220"
     CDS             7804..8754
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (26-313). E-value: 3.2e-53.
                     Score: 172.8. Matches aSDomain:
                     nrpspksdomains_C6W96_RS00225_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060646.1"
                     /locus_tag="C6W96_RS00225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_00220"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_199854314.1"
                     /sec_met_domain="ADH_N (E-value: 2.8e-08, bitscore: 32.2,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 4.1e-16, bitscore:
                     57.8, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKNESMRAISQSVYGGPGVLEEVRLPRPVAGPSQIVVAVRAAGV
                     NPTDWKHRSQGMFLDRLPLVLGWDVSGVVESVGFGVTLFKPGDEVFGMLPYPFGVGSHA
                     EFTVGPARAFAHKPEGVDHVQAAAVPLAALTARQALVDTADVRPGQRVLIHAAAGGVGH
                     LAVQIAKSLGAYVIGTASAAKHDFVRGLGADEVIDYRTVDFGDAVSDVDMVLDPISGET
                     RARSLDVLRPGGTLVSLLPGGDPAEAAKAAAREIRVENLLVEADHAGMTAIADLIRAGE
                     LRAHVEASFPLAEAAKAHALGETGRTTGKLVLVVS"
     aSDomain        7882..8742
                     /ASF="ER configuration inconclusive"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C6W96_RS00225_PKS_ER.1"
                     /evalue="3.20E-53"
                     /label="C6W96_RS00225_PKS_ER.1"
                     /locus_tag="C6W96_RS00225"
                     /protein_end="313"
                     /protein_start="26"
                     /score="172.8"
                     /tool="antismash"
                     /translation="PRPVAGPSQIVVAVRAAGVNPTDWKHRSQGMFLDRLPLVLGWDVS
                     GVVESVGFGVTLFKPGDEVFGMLPYPFGVGSHAEFTVGPARAFAHKPEGVDHVQAAAVP
                     LAALTARQALVDTADVRPGQRVLIHAAAGGVGHLAVQIAKSLGAYVIGTASAAKHDFVR
                     GLGADEVIDYRTVDFGDAVSDVDMVLDPISGETRARSLDVLRPGGTLVSLLPGGDPAEA
                     AKAAAREIRVENLLVEADHAGMTAIADLIRAGELRAHVEASFPLAEAAKAHALGETGRT
                     TGKLVL"
     CDS_motif       8248..8310
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6W96_RS00225_0001"
                     /evalue="1.90E-08"
                     /label="PKSI-ER_m5"
                     /locus_tag="C6W96_RS00225"
                     /protein_end="169"
                     /protein_start="148"
                     /score="26.6"
                     /tool="antismash"
                     /translation="GQRVLIHAAAGGVGHLAVQIA"
     gene            complement(8805..9416)
                     /locus_tag="C6W96_RS00230"
                     /old_locus_tag="C6W96_00225"
     CDS             complement(8805..9416)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="C6W96_RS00230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_00225"
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_107409705.1"
                     /transl_table=11
                     /translation="MTNALTIAAAGFGTGLSLIVAIGAQNALVLRQGVRRDGVLAVVGI
                     CALSDMVLITLGVAGVGAVVVAWPSALTVVGLLGGLFLIAYGLLAARRVLRPSALHAAD
                     APAPSRRGVVLTCLAMTWLNPHVYLDTVFLLGSVAADQGPSRWTFGLGAILASLCWFVT
                     LGFGARLLSGVLARPSAWRVLDGLVAVMMIGLGTKLVLGA"
     misc_feature    9454..9467
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.34"
                     /tool="antismash"
     gene            9484..10425
                     /locus_tag="C6W96_RS00235"
                     /old_locus_tag="C6W96_00230"
     CDS             9484..10425
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060648.1"
                     /locus_tag="C6W96_RS00235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6W96_00230"
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_107409706.1"
                     /transl_table=11
                     /translation="MMSELPLDQVRTLLAVVDEGTFDAAANVLRLTPSAVSQRVKALEQ
                     RTGRVLLMRTKPVRPTESGEVVVRFARQLSRLERDARTALGMSGPGEPTPLSIAVNSDS
                     LATWFLPALRRVPQELDLAYELRREDQDHTAALLREGLVMAAVTSSPEAVTGCSVRRLG
                     RMRYLPVAEPAFADRWLGRRAGADVRELIEDAPVVGFDRRDDLQDSFVRRLVPGARASS
                     RRHLVPSSEGFASAVAAGMGWGMVPDVQAEPLLREGRLVLLTPDHTVDVPLHWQQWKLD
                     SPALATVAEAVAAEAAETLAEGRPIAPRPLRG"
ORIGIN
        1 ggcggcccgg aggccagtcg cacccggtcg gcctggaagg cccgcatcgc ttcctccagc
       61 gcgccggcgg gcggcgggcg gccgccggtg cgcagaaacc gggcgcaggc ggtgcacagc
      121 tccgcgaccc ggaccaggag cgcggcggtc tcggggtcgg ccgggacggg cggggcttcg
      181 gcgagggtgg ccaactggtc gaggagtctg cgggcggagc ggccggcctg ttccagtgcg
      241 cggtcggtgc gcccggcgcc ggcgggccgt tccgccaggg gtacgtccgc gaggcgcagg
      301 gtcgatccgg cgtcccgcag ccgttgcggt ggtgcgcctg cgggtgcggc cccgcgcgcc
      361 gcctcgtcga gggcggcggc cagccgttcc cggtaggagg gtgcggccgg atcgggcagc
      421 aggatctcgc acgcggcgag gagcagtgcg ccggccgtga gccccgccag ccgctccacg
      481 agcgtgtcgg gggcgtacgg cgggaagcag gcgaggatgt agaagagctg gaggcctggg
      541 gcgatgccgg ccgggcgggg tccggccacg gccgtgaacg ccagcaggaa tccgatgacg
      601 agcatgccgc cgacggcggc ccaggtgtcg acggcgagca gggttcccag ggtcgccagg
      661 atcagtgccg ggggcagcgc ccggagcatg acggaggacc gttgagggcc ggagccgggg
      721 accacggaga gcagccccat ggcgatgggt gcgaagagcg cgtacagggc ggcgaccggg
      781 agcccggctc cgtacagcag ggggtagaag cccacgctcg ccgcgacggt gacccggacg
      841 gcccggcgga ggaccgtcgc ccgctgcggc cgggcgatca ccttgccgcc ccctccgggc
      901 gcggctcggg gcgggtgctc cgcgaggtcc cggccggggg gagccgctgt gcgctcatcc
      961 ggcacgctcc tctcgcgggg ccgggccctc cggtgcggcg gcccgctcgg ccgccttccg
     1021 ccattgtcgg ccggaccggg ccgcccgcgc gcgacgaagg cgtccgggga gcatccgagg
     1081 tgctcgggcc ggacgccttc ggtggagggg ccgctcgggg gcggcgcgcg tcagatggtc
     1141 gcggtgtcga tcacgaagcg gtagcggacg tcgctcgcga gaacccgctc gtacgcctcg
     1201 ttgacctggc tcgcgtcgat cacctcgatc tcggcgccga gcccgtgcac ggcgcagaag
     1261 tccagcatct cctgggtctc cttgatcccg ccgatcgccg aacccgcgat cgaccggttg
     1321 cccaggatca gggagaagag gttcagcgag atcggctcct cgggggcgcc cacgttgacg
     1381 agggtgccct cggtgcgcag caggccgagg taggcgccga agtccagcgg ggccgagacc
     1441 gtggagagga tgacgtcgaa ggttccggcc agttcctcga aggtcttggg gtcgctggtc
     1501 gcgtagtagt ggtcggcgcc cagccccagc ccgtcgtcct tcttccgcag cgactgggag
     1561 aggacggtga cctcggcgcc gagcgcgtgc gcgatcttga cggccatgtg gccgagaccg
     1621 ccgaggccga cgacggcgac cttcttgccg gggcccgcgc cccaccgctt gagcggggag
     1681 taggtggtga tgcccgcgca caggagcggc gcggcctcgt cgagggcgat gccctcgggg
     1741 atggtgacgg cgaacttctc ggtcacgacg aggtgcgtcg agtagccgcc gtaggtgggc
     1801 tcgccgctgc ggtcgagggc gttgtacgtc tgggtgttgc cctcggcgca gtgctgctcg
     1861 cggcccatca ggcaggcgtc gcactgtccg caggagtcga ccatgcagcc gacgccgacg
     1921 cggtcgccga ccttgaaccg ggtgacgccg gagccggtct cggcgacgat cccggcgatc
     1981 tcgtggccgg gcaccatcgg gaagatgccc tcgccccagc cgtcgcgggc ctggtggatg
     2041 tcggagtggc agatgccggc gaacttgatg tcgatcagga tgtcgaactc gcccaccgga
     2101 cgacgctcga tcgtggtgcg ctccagcggg gccttggcac ggggagcggc gtaggcggca
     2161 acggtggtca tgccggaggt tctcctctgg gtgatgcggt aggaacgtgt cccagcctca
     2221 ctccgtgtcc ggtgtttacc caggccaccg ctttgcctac gtccagtggt cctactactg
     2281 acggggacag gttcggccgc ctacgaccac gcggggcagg aataatggaa cccatggacg
     2341 atcagccgga ggccgccgca cccgagccgc tggaccggcg tgcggagctc agcgagttcc
     2401 tgcgcacccg cagggccaag cttcagccgc aggacgtggg actgccggag ttcggccggg
     2461 cccgtcgggt gccggggctt cgccgggagg agctggcgca gctggccggg gtgtcggtgg
     2521 cgtactacac gcgtctggag cagggcaacg ggcgcaatgt gtcggccgag gtgctggacg
     2581 cgatcgcgcg tgcgctgcgg ctcaccgacg ccgagcacgc gcatctgaac catctggcga
     2641 ggccggcccg gcacaagaag aagcgccggc cggcgaaggt gcagcgggtg cggaccgggc
     2701 tgctgtatct cctcgacagc atggagggca ttcccgcgta cgtggccggg gcgcggtccg
     2761 acatcctcgc ctggaatccg atggcggcgg cggtgttcgg caactgggcc gcgctgccgg
     2821 cggccgagcg gaactgggct cgcctggtgt tcctctcccc cgcctaccgg gacctgttcc
     2881 tcaactggga ctccaaggca tcggacatgg tcagctatct ccggctgtac gcggggcgcc
     2941 accccgacga tccgcagttg tcggcgctgg tgggcgaact ctcgctcaag agcgaggagt
     3001 tccggcggct gtgggccacg cacaacgtca aggagaaggg ccacggcatc aagctgatcc
     3061 ggcatccgct cgtcggcgac ctgactctgt cgtacgagac gctgaatctg ccggacgacg
     3121 aggaccagca gttggtgacc taccacgcgg agccggggtc ggaatcggcc caggccctgc
     3181 gcctgctggc gagctggggc gccgacgcgg gccgggcaca tgtgatcagc ggcgcgggcc
     3241 ggagtccgga cgacgagcgg tgaggacgaa ctcccggacg ctacggtcct gctgacgact
     3301 ggtcaggtcg gttccgccgt cagcgctgcc gtctgctccg cctcgccgac ggcggacgcc
     3361 gcgagtgcgg gggcccgggc cgacgccacc gcgcccgtcc cggccgggtc caggagcggc
     3421 agggtcttcg cggcggcgag ccggggcagc agcaactgcc agaaaccggt gagccggtac
     3481 ggggacagcc actcggtgtc gtgacggccg aggacctcga atccggccgt cacggcgacg
     3541 gtcgcggctg ccgcgtcctc gcgggcgatg tcgtcggcaa gggttccggc ggcggccgcc
     3601 tcgtccagca gctcccgcac gcggtggtgc cactccatcc gcgggtccgg ggcggcggcc
     3661 cgctcgccgt cgcagctcag ccggaatccg gcacggctca ccgggtcccc gcgcagcagt
     3721 tccacaaggg cgtgcgaggt gtcgacgagt gcttgcaggg cggtgtccgt cccgcgccgg
     3781 atcgcggcgg acatgtcgcg cagcccccgg gacgcctccg ccaccacggc ttccgcgacc
     3841 gcggccttgt tctcgaagtg aaagtgcagc gcccccgtgc tgacgcccgc gccggagctg
     3901 atcagcgtca gcctggcctg ggcgtaaccg tgctgttcga aaacgacggc ggcggagcgg
     3961 atcagcgcct ggcgggtccg ggtggcgcgc tcctgtttgg tcatggagtt atcaaaccaa
     4021 cttaccggtt tcatttccag ggaacgagac aatggcccat tccctgaccg gtcgggcggt
     4081 tcgacagtcg ggcgttcggc gcgctttcgc caagccgggc ggccgctcct atccggtcct
     4141 atccggctgc gaacgcgtcc gcgtggtcgg ccgaccaggt gcggtacgtc ctggccccgc
     4201 ggcccgtcag ctcctggacc gtgggcacca cggccgcctt cgcgcccgcc cgctgtcgct
     4261 ccgcgctctc caggaaggct tccgcgacgg gccgcgggta cttcgccagc agggccgcac
     4321 gtgcggcgtc caggcccagt tcctcgaagc gcagcggccg ccccagcacc cgggagagct
     4381 gagcggtctg ttcccgcgcg gtgatcgcct ccgggcccga gacggcgtac gcccgtccct
     4441 cgtggccggt gccggtgagt acggcgacgg ccaccgcggc gacgtcgcgg gggtcgacgc
     4501 acgcgaccgg ggcgtcgccg tacagggcgc gcaccacccc ggccgagcgg atgccgggcg
     4561 cccaggacag cgtgttggac atgaacgtcc tggggcggac gaaggtccag gcgatccccg
     4621 actccctgat cgcctcttcg ttctcccgct gacgccgggt gatgaagtcg tcggcgcccg
     4681 gttcctccac cgccatcatg gagagcttga cgaggtggcg gacgcccgcc gctcgggccg
     4741 cctcggcgac gtgctcgtcg tcgggctccg tggggctgtt cgtcaccagg aacagcgagt
     4801 tgacgccccg cagggcccgg tcgagggcgg gacggtcccc gtacgccccc tcgacgacct
     4861 cgaccccgtc cccgcgtacc gtcacgcggt ccgggcggcg ggcgagaatc cgtacgggac
     4921 cggtggccgc cagcagtgcg gcgacctcgc ggcccaccgc tccggtcgca cccgtcacga
     4981 gaatcactgg gaaagacctc tcagccgtcg gcccggggac cggacaggag tgtggagaag
     5041 accggttcgt cgtcctgatg acctgtcacc cggaccgtca ccgtgtcctc ggcctcttcc
     5101 ggctggatca ccgcgctgat ccagcacggg ctgccgaact cggcgtaccg gtggaaccgg
     5161 gtgctcgcct ccgccggaac gatgccgccg ggccccgccg cgaggcaggc cgcctgacgg
     5221 gccgcctcca gcagcagcat gcccggcaca tggtcgttgg ggcgctggaa cagcgtcggg
     5281 tgccgggtgt ccacgcgcag ctcccagacg ccttcgcggc cggtgtccga gagcaccacg
     5341 tcctcggtcc tgctccggcc cgcgcgggcg gcgtcgaccg gcgccgcctc cggtatggat
     5401 atcccctcgg cgggggcgtc gccgcgcatc cgccggtaca ccttggggct cgtgaagcgg
     5461 gtcgcgccca tccccgtggc cgccagctcc ccgccccggt gcaccgccca gcccacgcgc
     5521 ccctgcacgg gcagcccgct ccgccacttc aggtcggagc agaacacctc cacgtctatc
     5581 tcggtggggg ctccgccgac gcccaagtgg tcctggtggc atacgtagtc caggtccgcc
     5641 atcaggaagt ggtagccgag cggaacgccg tacccggcgt ggaaggcgag catggccgcc
     5701 tgccgcatcg cttcggcgac cagcagcgga tcgtgccggt cgccgcccac cggggcgaag
     5761 aacgggtgat cgtgcggcag gaccgccgcc accgagaacc gatcgtgtgc cagtcggacc
     5821 cagttgcggg gaaacgcgtc ctccggcctg gtccggtgca ccatgtcgat ccccacggga
     5881 tgcaccatgt cggcctccga atccattgcc ggcaccgagt cgatcagggc tgctgcttcg
     5941 ggcataactc cccccagggg tcatgtgatg gcgagccgtt cctgcgatgc acgtgcgctg
     6001 ataagataca gactcagcgg ttttttttct atccccggca gggtcgcccc tccccacacc
     6061 gtcccggccg tttcccgggc catccggccc agcaaaggag acaggatggc caagcaggac
     6121 cgggcgatcc gcacccgcca ggcaatcctg atggcagctg cgcaggcttt cgagaaatac
     6181 ggttatcaag ccgcgacgat caccgaaata ctcaaaagcg ccgacgtgac gaaaggcgct
     6241 ctctattttc attttccgtc caaggaagat ctcgcgctcg gtgttctcca tgcccaggag
     6301 ccgccgcagg ccgttccgga gcagtcactc aaactccagg aactcatcga tctggggatg
     6361 ctgttcagcc accgcctgca gaccagtctc ctcgcccgcg cgggcgtgcg cctctccatg
     6421 gaccagcagg cccatggcct cgaccgcagc ggccctttcc tccgctggca ggaagccacc
     6481 caccacctcc tcgcgcaggc caaacacaac ggtgagctgc tcccccacgt caaccccacc
     6541 gagacggccg acctgtacgt ggccgccttc acgggcaccc aggccgtatc ccagactctc
     6601 accaactacc aggacctgca gcgccgccac atcacgctcc agcagcacat cctccccagc
     6661 atcgccgcgc cctcgatcct caccgccctc gatctgacgc ccgcgcgcgc cgaacgtctc
     6721 agccgcctgg ccccggagga ctgaaccggt cggggcgccc gtgcgccctc aaggccgccc
     6781 cgccgcgaaa accgcgccac gaaacgtacg ccggtaggcc agtggagtga cgccgatggc
     6841 ggcgtgcaga tgctggcgca gtgacgcgcc ggtggcgaac ccgacctggc ccgcgatctc
     6901 gtccaccgcc aggtccgtgg actccaggag atgccgtgcc cggtcgaccc gctgctggac
     6961 gatccaccgg cccgggctca tgccgatctc ctcgttgaag cgtcggacga aggtgcgccg
     7021 gctcatccgg gcgtgctcgg cgaggctcgc cagactcagt ggttcctgga ggttgcgcag
     7081 cgcccagtcg cgggtcgcgg cggtgccgtt cgccgccgga gcgggcaccg gctgctcgat
     7141 gtactgggcc tgccctccgt cgcgccaggg cggtacgacg cagagccggg cgacccggtt
     7201 ggcgatctcg ctgccgtggt ccgcgcggac gaggtgcagg cagacgtcga cgccggaggc
     7261 cgccccggcc gaggtcagga cgcccccgtc gtcgatgaag aggacgcccg ggtcgagccg
     7321 cacctggggg taccaggacc ggaagagctc cgcgagcgcc cagtgggtgg tggcccgtcg
     7381 gccgtccagc agtccggccg aggcgaggag gaaggcgccg gtgcagatgg agacgatgcg
     7441 ggtgccgggg cgtacggccg cgagcgcggc gagggcgtcc gcaggcgcct cccgggtgag
     7501 gagagaggtg tcgaagggcg ggatgacgag ggtgtccgcc gtcgccagca cctcgggacc
     7561 gtgctcgacg gtgatcgaga agtcggcgtt ggtgcgcacg gggcggccgt cgaccgtgca
     7621 ggtcacgacg gagtagcggc cgtcggccgc gccgaggacc cggttgggga tgccgagctc
     7681 gaagggatac accccgtcga gggcgagtac ggcgacgcgg tggacgtttc ccatggcacg
     7741 atcctgtcga aagatggcaa tcatgccatg gtacgccggg cgtacaggcg tgaggctgga
     7801 cgcatgacga agaacgagag catgcgcgcc atcagccaga gcgtctacgg aggtcccggg
     7861 gtactggagg aggtccgtct tccccggccc gtcgcgggac cgagtcagat cgtggtcgcc
     7921 gtccgggcgg ccggagtgaa ccccacggac tggaagcacc ggagtcaggg gatgttcctg
     7981 gaccggctgc cgctggtcct gggctgggac gtgtccgggg tcgtggagtc ggtgggcttc
     8041 ggggtgaccc tcttcaagcc gggcgacgag gtgttcggca tgctgcccta cccgttcggt
     8101 gtcggctccc acgccgagtt cacggtcggc ccggcccgcg ccttcgccca caagccggag
     8161 ggcgtcgacc acgtccaggc ggccgccgtc ccgctcgccg cgctcaccgc ccgtcaggcg
     8221 ctcgtcgaca cggcggacgt gcgccccggg cagcgggtgc tgatccacgc cgccgccggc
     8281 ggggtcggcc acctcgccgt acagatcgcc aagtcactgg gggcgtacgt catcggcacc
     8341 gcgagcgccg ccaagcacga tttcgtgcgc gggctcgggg cggacgaggt catcgactac
     8401 cgcaccgtgg acttcgggga cgcggtgagc gatgtcgaca tggtgctcga cccgatctcg
     8461 ggcgagaccc gggcgcgttc gctcgatgtc ctgcgcccgg gaggcacgct cgtgtcgctg
     8521 ctgccgggcg gcgacccggc cgaggcggcg aaggccgccg cgcgggagat ccgggtggag
     8581 aacctgctgg tcgaggcgga tcacgcgggc atgacggcca tcgccgatct gatccgggcc
     8641 ggtgagctgc gcgcccatgt cgaggcgtcc ttcccgttgg cggaggctgc gaaggcgcac
     8701 gcgctgggcg agacgggccg caccaccggc aagctcgtcc tcgtggtgtc gtagcccgcg
     8761 gggcgcccgg cgcctcctcc cgctggggaa gaggcgccgg gcgttcaggc gcccagcacc
     8821 agtttcgtac cgaggccgat catcatcacg gcgaccaggc cgtccagcac ccgccatgcg
     8881 gagggccggg cgaggactcc gctcagcagc cgggcgccga acccgagggt cacgaaccag
     8941 cacaggctgg cgaggatcgc gccgaggccg aaggtccagc gcgaggggcc ctggtcggcg
     9001 gcgacggacc cgagcaggaa caccgtgtcg aggtacacgt gggggttgag ccaggtcatc
     9061 gccaggcagg tcagcacgac gccccggcgc gacggggccg gggcgtccgc cgcgtgcagg
     9121 gcggacgggc gcagcactcg gcgggcggcg agcagtccgt aggcgatcag gaacaggccg
     9181 ccgagcaggc ccacgacggt gagcgcggag ggccaggcga cgacgaccgc gccgacgccg
     9241 gcgacgccca gcgtgatgag caccatgtcg gacagggcgc agatgccgac caccgcgagc
     9301 acgccgtccc ggcggacgcc ctggcgcagc accagggcgt tctgggcgcc gatggcgacg
     9361 atgagggaga ggccggtacc gaatccggcg gccgcgatgg tcagggcgtt cgtcatgcca
     9421 ccgacgctac ggagcgcgac aacgcggcgt ccagctaaat attcttacgt atcattagca
     9481 accatgatgt ccgagcttcc tctcgatcag gtacgcacgc tgctggccgt ggtcgatgaa
     9541 ggcactttcg acgcggcggc gaacgtgctg cggctgacac cgtcggcggt cagccagcgg
     9601 gtgaaggcgc tggagcagcg cacgggccgg gtgctgctga tgcgtacgaa gccggtgcgg
     9661 cccaccgagt ccggtgaggt ggtggtgcgc ttcgcccgcc agctgtcccg gctggagcgc
     9721 gatgcgcgga cggcgctcgg gatgtccggg ccgggcgagc cgacgcccct gtcgatcgcg
     9781 gtgaactcgg actccctggc gacctggttc ctgcccgccc tgcggcgtgt accgcaggag
     9841 ctggacctcg cctacgaact gcgcagggag gaccaggacc atacggccgc cctgctgcgg
     9901 gaggggctgg tgatggccgc ggtgacgtcg tcgccggagg cggtgacggg gtgctcggtg
     9961 cgcaggctcg gccggatgcg gtacctgccg gtcgccgaac ccgctttcgc cgaccggtgg
    10021 ctggggcggc gggcgggcgc cgatgtgcgc gagctgatcg aggacgcccc ggtggtcggc
    10081 ttcgaccggc gcgacgatct gcaggactcc ttcgtgcgcc ggctcgtccc cggggcccgg
    10141 gcgagttccc ggcgtcatct ggtgcccagc tcggaggggt tcgcgagcgc cgtggcggcg
    10201 ggcatgggct ggggcatggt gcccgacgtc caggcggagc cgttgctgcg cgagggccgg
    10261 ctggtgctgc tcactccgga ccacaccgtg gacgtaccgc tgcactggca gcagtggaag
    10321 ctggactcgc ccgccctggc gacggtggcc gaggcggtgg cggcggaggc cgccgagacg
    10381 ctcgcggaag gacggccgat tgcccctcga ccccttcgag gttaggttta cctaactcaa
    10441 cagggaggtg cggaacatga cggatggtca gggtcaggag ccccgcaaca acgaggacgc
    10501 gccggtggat tggcaggcgc gcggggcctt gtcacggctc gtgctcggtc agatggccac
    10561 gcaggcgctg ggcgcggcgg tgcggttcga cgtcttcgac cggatcgggc cgggcacgct
    10621 gagcgccgac gagctcgccg aatccctcgg cacccatccg caggccacgc tgcgtctgct
    10681 gcgcgccctg gccggactcc aactgctgtc cgaaccggaa ccgggcctct tccggaccac
    10741 cgcggcgggc gacttcctgc gcacgggcac ggccggctcg atggccgcga tggcccggat
    10801 gttcaccgat ccggtcatgc tgcggggctg ggacctgctc gacgagagcg tgcgcacggg
    10861 ccggaccacg ttcgacacgg tgttcggg
//
