LOCUS       NZ_PVWC01000017         1355 bp    DNA     linear   CON 05-SEP-2024
DEFINITION  Bacillus halotolerans strain DGL6 NODE_18_length_1355_cov_37.7345,
            whole genome shotgun sequence.
ACCESSION   NZ_PVWC01000017
VERSION     NZ_PVWC01000017.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus halotolerans
  ORGANISM  Bacillus halotolerans
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., Wu,H. and Gao,X.
  TITLE     Genotypic and phenotypic analysis of antagonistic Bacillus spp.
            isolated from rhizosphere soil of plants in Tibet
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Borriss,R., Lasch,P., Wu,L., Wu,H. and Gao,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2018) UG, NordReet, Marienstr. 27a, Greifswald
            17489, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. A5-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38.3x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003003575.1-RS_2024_09_05
            Annotation Date                   :: 09/05/2024 04:49:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,703
            CDSs (with protein)               :: 3,703
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 9, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 8, 1 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 90 of 137
            Pseudo Genes (incomplete)         :: 71 of 137
            Pseudo Genes (internal stop)      :: 22 of 137
            Pseudo Genes (multiple problems)  :: 40 of 137
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:16:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1355
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PVWC01000017.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..1351)
                     /NRPS_PKS="Domain: AMP-binding (5-226). E-value: 9.5e-62.
                     Score: 200.8. Matches aSDomain:
                     nrpspksdomains_C6372_RS19580_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (328-396). E-value: 2.5e-26. Score:
                     83.6. Matches aSDomain: nrpspksdomains_C6372_RS19580_PCP.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_174754419.1"
                     /locus_tag="C6372_RS19580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C6372_19575"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_142396308.1"
                     /sec_met_domain="AMP-binding (E-value: 2.4e-79, bitscore:
                     252.8, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4e-19, bitscore:
                     55.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 7.4e-13, bitscore:
                     34.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEEDDIIMMKTSFSFDASVWQLFWWTLSGASAYLLPSGWEKDPAL
                     MVKAIREEGVTTAHFIPAMLNSFLDQAEIEAPRSLKRVFAGGEPLAPHTAARFASLLPE
                     TSLVHGYGPTEATVDAAFYACDPELDKNRLRLPIGKPVPGARLYVLDPHLAVQPVGVAG
                     ELYIAGAGVARGYLNRPELTEERFLDDPFYPGERMYKTGDLARWLPDGQVEFLGRLDDQ
                     VKIRGYRIEPGEIEAALRSIEGVREAAVTVRTESGEAELCAYAEGLERNEVRKQLETLL
                     PGYMIPAHIIEMEQWPVTPSGKLDRKALPAPDGAADRETYTAPRNLTEMKLSQLWEEVL
                     KSGPIGIHDNFFDRGGHSLKATALVSRIAKEFGVQVPLKDVFAHPTVEGLASVISEGME
                     SPYEAIKPAEKRETYPVSSAQKRMYVLQQLEDGGTGYNMPAVLELEGKLDLT"
     source          1..1355
                     /collection_date="2005"
                     /db_xref="taxon:260554"
                     /geo_loc_name="China: Qinghai, Qinghai lake"
                     /isolation_source="salt lake"
                     /mol_type="genomic DNA"
                     /organism="Bacillus halotolerans"
                     /strain="DGL6"
                     /submitter_seqid="NODE_18_length_1355_cov_37.7345"
     gene            complement(<1..>1355)
                     /locus_tag="C6372_RS19580"
                     /old_locus_tag="C6372_19575"
     region          1..1355
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1355
                     /SMILES="NC(CCC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1355
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1351](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1351)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     aSDomain        complement(162..365)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C6372_RS19580_PCP.1"
                     /evalue="2.50E-26"
                     /label="C6372_RS19580_PCP.1"
                     /locus_tag="C6372_RS19580"
                     /protein_end="396"
                     /protein_start="328"
                     /score="83.6"
                     /tool="antismash"
                     /translation="EMKLSQLWEEVLKSGPIGIHDNFFDRGGHSLKATALVSRIAKEFG
                     VQVPLKDVFAHPTVEGLASVISE"
     aSModule        complement(162..1334)
                     /complete
                     /domains="nrpspksdomains_C6372_RS19580_AMP-binding.1"
                     /domains="nrpspksdomains_C6372_RS19580_PCP.1"
                     /locus_tags="C6372_RS19580"
                     /monomer_pairings="Glu -> Glu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(642..707)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6372_RS19580_0002"
                     /evalue="2.00E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="C6372_RS19580"
                     /protein_end="236"
                     /protein_start="214"
                     /score="42.0"
                     /tool="antismash"
                     /translation="FLGRLDDQVKIRGYRIEPGEIE"
     aSDomain        complement(672..1334)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C6372_RS19580_AMP-binding.1"
                     /evalue="9.50E-62"
                     /label="C6372_RS19580_AMP-binding.1"
                     /locus_tag="C6372_RS19580"
                     /protein_end="226"
                     /protein_start="5"
                     /score="200.8"
                     /specificity="substrate consensus: Glu"
                     /tool="antismash"
                     /translation="IIMMKTSFSFDASVWQLFWWTLSGASAYLLPSGWEKDPALMVKAI
                     REEGVTTAHFIPAMLNSFLDQAEIEAPRSLKRVFAGGEPLAPHTAARFASLLPETSLVH
                     GYGPTEATVDAAFYACDPELDKNRLRLPIGKPVPGARLYVLDPHLAVQPVGVAGELYIA
                     GAGVARGYLNRPELTEERFLDDPFYPGERMYKTGDLARWLPDGQVEFLGRLDDQVKIR"
     CDS_motif       complement(789..878)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C6372_RS19580_0001"
                     /evalue="1.60E-19"
                     /label="NRPS-A_a6"
                     /locus_tag="C6372_RS19580"
                     /protein_end="187"
                     /protein_start="157"
                     /score="61.9"
                     /tool="antismash"
                     /translation="PVGVAGELYIAGAGVARGYLNRPELTEERF"
ORIGIN
        1 tgtaagatcc agctttcctt ccagctccaa gactgccggc atattgtagc ctgtgcctcc
       61 atcctccagc tgctggagca catacatccg cttttgggcg gaggaaaccg ggtaggtctc
      121 gcgtttttca gccggcttaa tggcttcata cggactttcc attccttcgc tgatgacaga
      181 cgccaaacct tctaccgtcg gatgggcgaa cacatccttc agcggcactt ggacgccgaa
      241 ttccttggcg atccgggaaa caagcgcggt ggcttttaag gaatggccgc cccggtcaaa
      301 aaagttgtcg tgaatgccga tagggccgct tttcagcact tcctcccaca gctgggaaag
      361 cttcatctct gtcaggtttc tcggcgctgt gtaggtttcc cgatctgccg ctccgtcagg
      421 agcaggcagc gcttttcggt cgagctttcc gcttggggta accggccatt gttccatctc
      481 aatgatatga gcagggatca tgtagcccgg cagcaaagtc tcaagctgct tgcgcacttc
      541 atttctttcg agtccttccg catacgcaca taattccgcc tcgccgcttt ctgtacgaac
      601 ggtcacagcc gcttcccgca caccttcaat gcttctgagc gccgcttcaa tttctcccgg
      661 ctcaatccgg tagccgcgga ttttcacctg gtcatccaga cggccgagga attcaacctg
      721 tccgtctggg agccagcgcg ccaaatcacc cgttttgtac atgcgttcac ccggataaaa
      781 tggatcatca agaaaacgtt cttctgttaa ttccggacga ttcaagtagc ctctggcaac
      841 cccggctccg gcgatataca attctcccgc tacgccgaca ggctgtacgg ctaaatgagg
      901 atctaataca tacagacgcg cgccaggcac gggttttccg atcggaaggc gtaagcgatt
      961 cttgtccagc tcaggatcac acgcgtaaaa tgcggcatca acggttgctt ctgtcggccc
     1021 gtagccgtga accagcgatg tctccggcag caaagaagcg aaacgggctg ccgtatgggg
     1081 agcaagcggt tcacctccgg cgaatacgcg cttcaggctt ctcggtgcct ctatttccgc
     1141 ttgatccaaa aagctgttca gcatggccgg aataaaatgg gccgttgtaa cgccttcctc
     1201 ccgaatggct tttaccatca atgccgggtc tttctcccag ccggacggaa gcagataggc
     1261 cgaagcacct gaaagcgtcc accaaaacag ctgccagaca gatgcatcaa aggaaaaaga
     1321 cgttttcatc ataatgatgt cgtcttcctc taaag
//
