LOCUS       NZ_PVTF01000025        11452 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Umezawaea tangerina strain DSM 44720 Ga0180964_125, whole genome
            shotgun sequence.
ACCESSION   NZ_PVTF01000025
VERSION     NZ_PVTF01000025.1
KEYWORDS    WGS; RefSeq.
SOURCE      Umezawaea tangerina
  ORGANISM  Umezawaea tangerina
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Umezawaea.
REFERENCE   1  (bases 1 to 98899)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase
            II (KMG-II): from individual species to whole genera
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 98899)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2018) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.9.0
            Expected Final Version            :: No
            Genome Coverage                   :: 86.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003002815.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:47:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,483
            CDSs (total)                      :: 9,418
            Genes (coding)                    :: 9,322
            CDSs (with protein)               :: 9,322
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 20 of 97
            Pseudo Genes (incomplete)         :: 83 of 97
            Pseudo Genes (internal stop)      :: 2 of 97
            Pseudo Genes (multiple problems)  :: 8 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:41:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 87447
            Orig. end                         :: 98899
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            PVTF01000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11452
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..11452
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11452
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[10000:11113](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11113)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    128..142
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.34"
                     /tool="antismash"
     gene            491..1948
                     /gene="sufB"
                     /locus_tag="CLV43_RS46030"
                     /old_locus_tag="CLV43_12578"
     CDS             491..1948
                     /GO_function="GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /GO_process="GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sufB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236603.1"
                     /locus_tag="CLV43_RS46030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV43_12578"
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="WP_106196890.1"
                     /transl_table=11
                     /translation="MTAAAEQRTPTTVQSEPLSQEETLASIGNYEFGWSDSDAAGTSAR
                     RGLDEDVVRDISAKKSEPEWMLEHRLKGLRLFEKKPMPTWGSDLSGINFDSIKYFVRST
                     EKQATSWEDLPEDIKNTYDRLGIPEAEKQRLVAGVAAQYESEVVYHQIREDLEAQGVIF
                     KDTDTALKEHPELFQEYFGSVIPSGDNKFSALNSAVWSGGSFIYVPKGVKVDIPLQAYF
                     RINTENMGQFERTLIIVDEGAYVHYVEGCTAPIYSSDSLHSAVVEIIVKKGGRCRYTTI
                     QNWSNNVYNLVTKRAKAEEGATMEWIDGNIGSKVTMKYPAVFLMGEHAHGEVLSIAFAG
                     EGQHQDAGAKMVHMAPHTSSTIVSKSVARGGGRTSYRGLVQINKRAHHSKSTVKCDALL
                     VDNISRSDTYPYVDVREDDVQMGHEATVSKVSAEQLFYLMSRGLTEDEAMAMIVRGFVE
                     PIARELPMEYALELNRLIELQMEGAVG"
     gene            1950..3128
                     /gene="sufD"
                     /locus_tag="CLV43_RS46035"
                     /old_locus_tag="CLV43_12579"
     CDS             1950..3128
                     /GO_function="GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /GO_process="GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sufD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699742.1"
                     /locus_tag="CLV43_RS46035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV43_12579"
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="WP_106196891.1"
                     /transl_table=11
                     /translation="MAVSTEAQHGLTDHTHGVGGTPVSSRADHFASFDVDAFEVPGGRE
                     EIWRFTPMKRLKNLHNGAPATGSATVEVKAAAGITVETVRRGDARLGVAGTPSDRIAAQ
                     AWSSFTDGTLLTVGKNEEVTEPTLVTVTGAGEGEVAYGHLQIRSELFSKAVVVVDHRGS
                     GAYADNVEFVVGDGAHLTVISVQDWADDAVHVSSHHASLGRDATFRHTVITLGGDVVRL
                     TPTVTYTGKGGDAELNGLYFADHGQHLEHRTFIDHKVSNCRSNVVYKGALQGEGAHTVW
                     IGDVLIRAAAEGTETYELNRNLVLTDGARADSVPNLEIETGEIVSAGHASATGRFDDEQ
                     LFYLQARGIPEDQARRLVVRGFFHEILLKITVPEVRERLEAAIEAELEAVGA"
     gene            3163..3471
                     /locus_tag="CLV43_RS46040"
                     /old_locus_tag="CLV43_12580"
     CDS             3163..3471
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699743.1"
                     /locus_tag="CLV43_RS46040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV43_12580"
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="WP_106196892.1"
                     /transl_table=11
                     /translation="MIKVCAFAELDDRKPFAADVDGNPVVVVRDGERVHALQDQCSHAA
                     VSLSEGEVTKQGIECWLHGSRFDLTTGEPSSPPASEPVDVYAVEVRDGDVYVAVPQD"
     misc_feature    complement(3489..3509)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.82"
                     /tool="antismash"
     gene            3498..4268
                     /gene="sufC"
                     /locus_tag="CLV43_RS46045"
                     /old_locus_tag="CLV43_12581"
     CDS             3498..4268
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sufC"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333168.1"
                     /locus_tag="CLV43_RS46045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV43_12581"
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="WP_106196893.1"
                     /transl_table=11
                     /translation="MATLEIKDLHVTVLTDEAPKEILKGVDLTIKAGETHAIMGPNGSG
                     KSTLSYAVAGHPKYTVTSGSVTLDGENVLDMSVDERARAGLFLAMQYPVEVPGVSMSNF
                     LRTAATAVRGEAPNLRHWVKEVKGAMSELDIDPAFAERSVNEGFSGGEKKRHEILQLGL
                     LQPKIAILDETDSGLDVDALRVVSEGVNRYKASGEVGVMLITHYTRILKYISPDFVHVF
                     ANGRIVESGGPELADVLENEGYVKYTGSAEAAKV"
     gene            4315..5610
                     /locus_tag="CLV43_RS46050"
                     /old_locus_tag="CLV43_12582"
     CDS             4315..5610
                     /EC_number="2.8.1.7"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_5 (30-411). E-value: 4e-138.
                     Score: 452.3. Matches aSDomain:
                     nrpspksdomains_CLV43_RS46050_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421257.1"
                     /locus_tag="CLV43_RS46050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV43_12582"
                     /product="cysteine desulfurase"
                     /protein_id="WP_106196894.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.3e-140, bitscore:
                     458.5, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 4.6e-12,
                     bitscore: 38.3, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTATPLDVVALREDFPILARTVREGKKLVYLDSGATSQRPTQV
                     LDAERRFLETSNAAVHRGAHQLAEEATDAYESARARIAEFVGASLDELVFTKNATEGIN
                     LVAYAMGNAATSGPEAQRFLLGPGDEILITEMEHHANLVPWQQLCLRTGATLRWIDVTA
                     EGRLDLSNLDELVTPRTKVVAFTHQSNVLGTINPVAELVAAARKVGALTVLDACQSVPH
                     SKVDFRALGVDFAAFSGHKMLAPSGIGVLYGRRELLEALPPFLTGGSMIEMVHMDRSTF
                     APPPQRFEAGVPMTSQAIGLGAAVDYLTAVGMDRVAAHEHLLTEAALAGLGEIPGVRII
                     GPRSTVDRGGAVSFGVEGVHAHDAGQVLDSLGIAVRVGHHCAWPLHRKLGVQASIRASF
                     YLYNDLDEVAALLDGVREAQRFFGVGPVPGQG"
     aSDomain        4405..5547
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CLV43_RS46050_Aminotran_5.1"
                     /evalue="4.00E-138"
                     /label="CLV43_RS46050_Aminotran_5.1"
                     /locus_tag="CLV43_RS46050"
                     /protein_end="411"
                     /protein_start="30"
                     /score="452.3"
                     /tool="antismash"
                     /translation="VYLDSGATSQRPTQVLDAERRFLETSNAAVHRGAHQLAEEATDAY
                     ESARARIAEFVGASLDELVFTKNATEGINLVAYAMGNAATSGPEAQRFLLGPGDEILIT
                     EMEHHANLVPWQQLCLRTGATLRWIDVTAEGRLDLSNLDELVTPRTKVVAFTHQSNVLG
                     TINPVAELVAAARKVGALTVLDACQSVPHSKVDFRALGVDFAAFSGHKMLAPSGIGVLY
                     GRRELLEALPPFLTGGSMIEMVHMDRSTFAPPPQRFEAGVPMTSQAIGLGAAVDYLTAV
                     GMDRVAAHEHLLTEAALAGLGEIPGVRIIGPRSTVDRGGAVSFGVEGVHAHDAGQVLDS
                     LGIAVRVGHHCAWPLHRKLGVQASIRASFYLYNDLDEVAAL"
     gene            5613..6056
                     /gene="sufU"
                     /locus_tag="CLV43_RS46055"
                     /old_locus_tag="CLV43_12583"
     CDS             5613..6056
                     /GO_function="GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /GO_process="GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sufU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071387.1"
                     /locus_tag="CLV43_RS46055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV43_12583"
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="WP_106196895.1"
                     /transl_table=11
                     /translation="MQLQQMYQEIILDHYKNPHGRGLRDPYDAESHQVNPTCGDEVTLR
                     VKLDGETVQDVSYDGQGCSISQASTSVLTDLVIGRSVGEAFKKFDAFVELMQSRGQLEP
                     DEDVLEDGIAFAGVSKYPARVKCALLGWMAFKDAVARSEGAKA"
     gene            6053..6448
                     /locus_tag="CLV43_RS46060"
                     /old_locus_tag="CLV43_12584"
     CDS             6053..6448
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421256.1"
                     /locus_tag="CLV43_RS46060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV43_12584"
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="WP_106196896.1"
                     /transl_table=11
                     /translation="MSEQDTEVVRGVEGLPVPPAPKADVAAIDDIEEAMRDVVDPELGI
                     NVVDLGLVYDLRVEDGNVAIIDMTLTSAACPLTDVIEDQTRQALTGGPGGGLVDDFRIN
                     WVWMPPWGPEKITDDGREQLRALGFTV"
     gene            complement(6887..8020)
                     /locus_tag="CLV43_RS46065"
                     /old_locus_tag="CLV43_12585"
     CDS             complement(6887..8020)
                     /EC_number="6.3.2.2"
                     /GO_function="GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1165: carboxylate-amine ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103584.1"
                     /locus_tag="CLV43_RS46065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV43_12585"
                     /product="glutamate--cysteine ligase"
                     /protein_id="WP_106196897.1"
                     /transl_table=11
                     /translation="MSKADTSQLPRPRTIFDQSLSVGVEEEFLLVDATTREVAPLAPEV
                     LATKSDGRFDLQAELTDFQVEIATPVCRSMAEVRSELVAARGALGAMAAEHGTRLVATG
                     TAVLGEVHPAPLTDDPRYRRMAVEYGRLIHDLTICGCHVHVGIPDEDAGVHISNHLRPW
                     LPVLLALSANSPFASGRDTGYASWRYLTWSPWPSAGAPPRFASAEEYHANTQLLQSSGA
                     AMDAATIYWDIRLSANHPTVELRVCDVAATVDEAVLIAALTRAIAVMALSSRVPALDVA
                     PHLLRAALWRAARDGIEGTNVNLATGELVQASTQLHALLTWARPALEASGDFDLATAAV
                     DRMLLDGSGAERQRRAFRQRGELSDVVDLLIRQTASK"
     gene            complement(8204..9919)
                     /locus_tag="CLV43_RS46070"
                     /old_locus_tag="CLV43_12586"
     CDS             complement(8204..9919)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419521.1"
                     /locus_tag="CLV43_RS46070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV43_12586"
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_106196898.1"
                     /transl_table=11
                     /translation="MPALLDDVRFAFQPLFNLHTGGVVAVEALARPHDGGVQDLLRAAH
                     GAGYLTNTDVALACRAVGSAADHELALPLHLNLMAMTLADRPEFLAPLFGALRDAGRSP
                     SSVVVEVGIPYSRARRGALLRGLELLRGNGFRIALDGVGEGDVPLSLLAGARPDLLKLD
                     REVVAALPDDPGQCAVVQGLLHIAEHTGAQLVAEGVETEAELASLRRLGVWLAQGNLLA
                     AASRRPRVDATIAAVLSEANDPEAVTRTGPIRRATGPRVTDFLHPATTLPEDATSERVR
                     ELLAGHPSLSGVVLVDPDGRPRWSVDRNRFLLAVTGPYGHALHARRDAARLADRPHLIG
                     TGTSAVELLDIVANTDRERTNDDLVVVDADDRCLGVVRVADVVRGIAELKVEQAAALNP
                     LTRLPGSDAIAREVDRRIMAGEIFAVGWLDVDGFKRVNDGVGFAAGDELIRGIGRTLSE
                     AAVPWPTAQVGHVGGDDFLVVAGLDEIVPLASRVLDTEFEAEGLAVTLSLATLVCAAGS
                     VPSYREVSRLLAPLKARAKSLRGTSWVLGRPGNDRVDLLRGTPVGESGSPRPAA"
     gene            complement(10001..11113)
                     /locus_tag="CLV43_RS46075"
                     /old_locus_tag="CLV43_12587"
     CDS             complement(10001..11113)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: Lycopene_cycl"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310672.1"
                     /locus_tag="CLV43_RS46075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV43_12587"
                     /product="lycopene cyclase family protein"
                     /protein_id="WP_211304888.1"
                     /sec_met_domain="Lycopene_cycl (E-value: 9.9e-53, bitscore:
                     172.0, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVIVLGGGPAGRALAGACATSGLETTLVDPRPERPWTATYGAWA
                     DELPSGTPVAAVAPVTRAIGVEAHVLDRAYAVIDNARLQVPHPDVLVETGKGVRTVVGP
                     RGTTVLLADGRTLTAALVVDATGAPPSRGRVEQTAAGVVVAATDAKALVAEGEALIMDW
                     RQPPTTGVDWPTFLYAVPLDGDRVLLEETSLARRPGLPVAELRTRLAARLAQHGVDLSD
                     VEEQVRFPVDLNRPAKLAFGAAAALVHPATGYSVATTLTLAPLVAAAIADALPRGPRAA
                     RAAARQVVWSPAAKVVHTMRRHGLRVLLAMPPEHVPEFFDLFFGLPEELRRAYLSDRED
                     VAGATKAMAELFRGAPWRLRRTMVLAPQDR"
ORIGIN
        1 gcggccaacg aggccgcgga cgtccggcga agctcttcct gctgaccgag acggggcgtt
       61 cccggttcgg gcacgcctac gacgacctcg ccgtcgccgc gttgcggttc ctcgccgaac
      121 agggcgggga agaggcggta cgggccttcg cgcaacgccg gatggccgcc atggtcgacc
      181 agcaccgcgc cgcgatcacg gccctgcccg acccggtcga cagggccacg gccctggcga
      241 gcgcgttgag cagggagggt tacgctgcct cgacgcgcga cgtgggcgcg ggagcgcagc
      301 tgtgccagca ccactgcccg gtcgcccacg tggcggccga gttcccgcag ctgtgcgaga
      361 ccgagacgga gaccttcgcc gagctcctcg gttcccacgt ccagcggctg gcgacgatcg
      421 cacgcggcga cgccgtgtgc accacgcaca tcccgaacac cgttaagacc ccagatggag
      481 gggagcccgc atgactgccg ctgccgagca gcgcacgccc accacggtgc agtctgagcc
      541 gctcagccag gaggagaccc tggcgagtat cggcaactac gagttcggct ggtccgactc
      601 cgacgccgcc ggtacgagcg cacgccgagg cctggacgag gatgtcgtca gggacatctc
      661 ggcgaagaag agcgagcccg agtggatgct ggagcaccgc ctcaaggggc tgcggctgtt
      721 cgagaagaag ccgatgccga cgtggggttc cgacctctcc ggcatcaact tcgactcgat
      781 caagtacttc gtgcgttcga ccgagaagca ggccacctcg tgggaggacc tgcccgagga
      841 catcaagaac acctacgacc ggctgggcat ccccgaggcg gagaagcagc gcctcgtggc
      901 cggtgtcgcc gcgcagtacg agtccgaggt ggtctaccac cagatccgcg aggacctgga
      961 agcgcagggc gtcatcttca aggacaccga caccgcgctc aaggagcacc cggagctgtt
     1021 ccaggagtac ttcggctccg tcatcccctc gggtgacaac aagttctcgg cgctgaactc
     1081 cgcggtgtgg tcgggcggct cgttcatcta cgtccccaag ggcgtcaagg tggacatccc
     1141 gctgcaggcc tacttccgga tcaacaccga gaacatgggc cagttcgagc gcacgctgat
     1201 catcgtcgac gagggtgcct acgtgcacta cgtcgagggc tgcacggcgc cgatctactc
     1261 ctcggactcg ctgcactcgg ccgtggtcga gatcatcgtg aagaagggcg gccgctgccg
     1321 ctacacgacg atccagaact ggtcgaacaa cgtctacaac ctggtcacca agcgcgccaa
     1381 ggccgaagag ggcgcgacca tggagtggat cgacggcaac atcggctcca aggtgaccat
     1441 gaagtacccg gcggtcttcc tcatgggcga gcacgcccac ggcgaggtcc tctcgatcgc
     1501 gttcgcgggc gagggccagc accaggacgc gggcgccaag atggtccaca tggcaccgca
     1561 cacctcctcg acgatcgtgt cgaagtcggt ggcgcgcggt ggtgggcgca cgtcctaccg
     1621 cggcctggtg cagatcaaca agcgggcgca ccactcgaag tccacggtga agtgcgacgc
     1681 gctgctggtg gacaacatca gccgctcgga cacctacccc tacgtcgacg tccgcgagga
     1741 cgacgtgcag atgggccacg aggccacggt ctcgaaggtg tccgccgagc agctgttcta
     1801 cctgatgtcg cgcggcctca ccgaggacga ggccatggcg atgatcgtgc gcgggttcgt
     1861 cgagcccatc gcgcgcgagc tgcccatgga gtacgcgctc gagctgaacc gcctgatcga
     1921 actgcagatg gaaggggccg tcggctgaca tggccgtttc aaccgaggcc cagcacggcc
     1981 tgacggacca cacgcacggc gtcgggggca ccccggtgtc gtcgcgtgcg gaccacttcg
     2041 cctcgttcga cgtcgacgcc ttcgaggtgc ccggcggccg cgaggagatc tggcgcttca
     2101 cccccatgaa gcggctgaag aacctgcaca acggcgcccc cgcgacgggg tcggccaccg
     2161 tcgaggtcaa ggcggcggcg ggcatcaccg tcgagaccgt ccggcgcggc gacgcgcggc
     2221 tcggtgtcgc gggcaccccc agcgaccgga tcgccgcgca ggcgtggtcg tcgttcaccg
     2281 acggcacgct gctgacggtg ggcaagaacg aggaggtcac cgagccgacg ctggtcaccg
     2341 tgaccggcgc cggcgagggc gaggtcgcct acgggcacct gcagatccgc tcggagctgt
     2401 tctccaaggc cgtcgtggtg gtcgaccacc gaggttccgg cgcgtacgcg gacaacgtcg
     2461 agttcgtggt cggcgacggc gcgcacctca ccgtcatctc ggtccaggac tgggccgacg
     2521 acgcggtgca cgtctcctcc caccacgcct cgctcggccg cgacgcgacc ttccggcaca
     2581 cggtcatcac gctgggcggc gacgtcgtcc ggctcacccc gacggtgacc tacacgggca
     2641 agggcggcga cgcggagctc aacgggctct acttcgccga ccacggccag cacctggaac
     2701 accgcacgtt catcgaccac aaggtgtcga actgccgcag caacgtcgtc tacaagggcg
     2761 cgttgcaggg cgagggcgcg cacaccgtgt ggatcggcga cgtgctgatc cgggcggcgg
     2821 ccgagggcac cgagacctac gagctgaacc ggaacctggt gctcaccgac ggcgcccgcg
     2881 ccgactcggt gccgaacctg gagatcgaga ccggcgagat cgtcagcgcc gggcacgcca
     2941 gcgccaccgg ccggttcgac gacgagcagc tgttctacct gcaggccagg ggaatcccgg
     3001 aggaccaggc ccgccgcctg gtcgtgcgcg gcttcttcca cgagatcctg ctgaagatca
     3061 cggtccccga ggtgcgcgag cgcctcgagg ccgcgatcga ggccgagttg gaagcagtag
     3121 gcgcgtgacg tgccgacgtg ctcgtcccgg cgggggcgca cgatgatcaa ggtgtgcgcc
     3181 ttcgccgagc tggacgaccg caagccgttc gcggccgacg tcgacgggaa ccccgtcgtc
     3241 gtcgtccgcg acggcgagcg ggtgcacgca ctgcaggacc agtgctcgca cgccgcggtg
     3301 tcgttgagcg agggcgaggt gacgaagcag gggatcgagt gctggctgca cggttcccgc
     3361 ttcgacctca ccaccggtga gccgtcctcg ccgcccgcct ccgagcccgt cgacgtctac
     3421 gccgtcgaag tacgcgatgg cgacgtgtac gtcgccgtcc cccaggactg accgcagtta
     3481 caggagaaca cagagccatg gccacgcttg agatcaagga cctgcacgtc acggtcctca
     3541 ccgacgaagc ccccaaggag atcctgaagg gcgtcgacct gaccatcaag gcgggcgaga
     3601 cgcacgccat catggggccc aacggctccg gcaagtccac gctgtcctac gccgtcgcgg
     3661 gccacccgaa gtacacggtg acctccggtt cggtgacgct ggacggcgag aacgtgctcg
     3721 acatgagcgt cgacgagcgc gcccgcgcgg gtctcttcct cgcgatgcag tacccggtcg
     3781 aggtgcccgg cgtctcgatg tcgaacttcc tgcgcaccgc cgccaccgcc gtccgcggcg
     3841 aggccccgaa cctgcgccac tgggtcaagg aggtcaaggg cgcgatgtcc gaactggaca
     3901 tcgacccggc cttcgccgag cgcagcgtca acgagggctt ctccggcggc gagaagaagc
     3961 gccacgagat cctccagctg ggcctgctcc agccgaagat cgcgatcctg gacgagaccg
     4021 actccggtct ggacgtcgac gcgctgcgcg tcgtgtccga gggcgtcaac cgctacaagg
     4081 cctccggtga ggtcggcgtc atgctgatca cgcactacac ccggatcctc aagtacatct
     4141 cgcccgactt cgtgcacgtg ttcgcgaacg gccggatcgt cgagtccggc ggccccgagc
     4201 tcgccgacgt cctcgagaac gagggttacg tgaagtacac cggcagcgcg gaagcagcga
     4261 aggtctgagt ttgttgcagt ggtcgaaccc gtactgagag gaggcgtcgg accagtgacc
     4321 accacagcca ctccgctgga cgtcgtcgca ttgcgcgagg acttcccgat cctggcgcgc
     4381 acggtccgcg agggcaaaaa gctggtgtac ctcgactccg gcgccacctc gcagcggccc
     4441 acgcaggtgc tcgacgcgga acggcgtttc ctggagacgt cgaacgccgc cgtgcaccgc
     4501 ggcgcgcacc agctcgccga agaggcgacg gacgcgtacg agagcgcgcg ggcgcggatc
     4561 gccgagttcg tcggcgcctc gctcgacgag ctcgtgttca ccaagaacgc caccgagggc
     4621 atcaacctcg tcgcttacgc catgggcaac gcggcgacct ccggcccgga ggcccagcgg
     4681 ttcctgctcg gccccggcga cgagatcctg atcaccgaga tggagcacca cgcgaacctg
     4741 gtgccgtggc agcagctctg cctgcgcacc ggcgcgacgc tccgctggat cgacgtgacc
     4801 gccgagggca ggctcgacct gtcgaacctc gacgagctgg tgacgccccg gaccaaggtc
     4861 gtggcgttca cccaccagtc gaacgtgctc ggcacgatca accccgtggc cgaactggtg
     4921 gccgcggccc gcaaggtcgg cgcgctgacc gtgctggacg cctgccagtc ggtgccgcac
     4981 tcgaaggtgg acttccgggc gctgggcgtc gacttcgcgg cgttcagcgg ccacaagatg
     5041 ctggccccgt ccgggatcgg cgtgctctac ggcaggcgtg aactgctgga ggcgctgccg
     5101 ccgttcctca ccggcggatc gatgatcgag atggtccaca tggaccggtc gacgttcgcg
     5161 ccgccaccgc agcggttcga ggcgggcgtt cccatgacgt cgcaggcgat cgggctcggc
     5221 gcggccgtgg actacctgac cgccgtcggc atggaccggg tcgccgcgca cgagcacctg
     5281 ctgaccgagg ccgcgctggc gggcctcggc gagatccccg gcgtgcggat catcggaccg
     5341 aggtccacag tggaccgcgg tggcgcggtg tcgttcggcg tcgagggcgt gcacgcccac
     5401 gacgccggac aggtgctgga cagcctcggc atcgccgtcc gagtcggcca ccactgcgcg
     5461 tggccgctgc accgcaagct cggcgtgcag gccagcatcc gggcttcctt ctacctctac
     5521 aacgacctcg acgaggtggc cgcgctcctg gacggcgtcc gcgaagcgca gcggttcttc
     5581 ggtgtaggcc cggttcctgg gcagggatga cgatgcagct gcagcagatg taccaggaga
     5641 tcatcctgga ccactacaag aacccgcacg gtcgcggtct gcgcgacccg tacgacgccg
     5701 agtcccacca ggtcaacccg acctgcggtg acgaggtcac gctgcgggtc aagctcgacg
     5761 gcgagactgt ccaggacgtc tcctacgacg gccagggctg ctccatcagc caggcctcca
     5821 cgtcggtgct gaccgacctg gtgatcggca ggtccgtcgg cgaggcgttc aagaagttcg
     5881 acgccttcgt ggagctcatg cagagccgcg gccaactgga acccgacgag gacgtcctgg
     5941 aggacggcat cgccttcgcg ggcgtttcga agtacccggc gcgcgtcaag tgcgcgctgc
     6001 tcgggtggat ggcattcaag gacgcggtcg cccgcagcga aggagcaaag gcatgagcga
     6061 gcaggacacc gaggtcgtgc gcggcgtcga agggctgccc gtgccacccg cacccaaagc
     6121 cgacgtcgcc gccatcgacg acatcgagga agccatgcgc gacgtggtgg accccgagct
     6181 gggcatcaac gtggtcgacc tcggcctcgt gtacgacctc cgcgtcgagg acggcaacgt
     6241 cgcgatcatc gacatgacgc tgacgtccgc ggcgtgcccg ctgacggacg tgatcgagga
     6301 ccagacgcgt caggcgttga ccggtgggcc cggtgggggg ttggtggacg acttccggat
     6361 caactgggtg tggatgccgc cgtgggggcc ggagaagatc acggatgatg ggcgggagca
     6421 gcttcgggcg ttggggttca cggtgtaggt gttgtttggt gttgggtgct ttgggcccgc
     6481 ttggcttcgg ctgggcgggt cttttgcgtt ggtgggtggg gtgtggggga agcccggccc
     6541 ccggcgcgcg attgtctcaa tgcttgatct tgcttgtcaa gacgataggg ctgtcttgac
     6601 aagcaagatc aagcaggagg gcgctgtgga tcgggggcag agggggagtc tggcttcgcc
     6661 agcatcgagc tgcgctcgac agggcatcct cggcttcgcc gtcggacaag gcatccttgg
     6721 cttcgccgtt ggacggggca tccgcgcttc gcggcggaca gaacatcctt gctgcgctgc
     6781 ggacaaggcc ccaggcgctt cgcgccggat gcgcgctggc gggctgcgcc cggcggggtg
     6841 gggttggtgc ttgctgtgcg ttggacaggc aagcggcctt gccgtcctat ttcgaagcgg
     6901 tctggcgtat cagcaggtct actacgtcgc tcagttcgcc ccgctgtcgg aatgctcgtc
     6961 gctgtcgttc tgcccctgag ccgtccagca gcattcggtc gacggctgct gttgccaggt
     7021 cgaagtcgcc ggaagcttcc agggcggggc gggcccaggt gagtagggcg tggagttgtg
     7081 tggaggcttg gaccagctcg ccggttgcga ggttcacgtt cgtgccttcg atgccgtcgc
     7141 gggcggcgcg ccagagggct gctcggagca ggtggggggc gacgtcgagg gcggggacgc
     7201 gggagctgag ggccatgact gcgatcgcgc gggtcaaggc ggcgatgagg actgcctcgt
     7261 ccacggtggc cgcgacgtcg cagacgcgca gttcgacggt cgggtggttg gccgaaagcc
     7321 tgatgtccca gtagatcgtg gccgcgtcca tggccgcgcc gctgctttgc aggagctggg
     7381 tgttcgcgtg gtactcctcg gccgaggcga atcgtggtgg ggcgcctgcg gatggccatg
     7441 gtgaccacgt caggtagcgc cagctggcgt agccggtgtc ccggccggag gcgaacgggg
     7501 agttggcgct cagtgcgagc agcaccggca gccacggtcg gaggtggttg ctgatgtgga
     7561 cgcccgcgtc ctcgtccggg atccccacgt gcacgtggca gccgcagatg gtcaggtcgt
     7621 ggatcaggcg tccgtactcg accgccatgc gtcggtagcg ggggtcgtcg gtcagcggag
     7681 cggggtgcac ctcgccgagc accgccgtgc ccgtcgcgac caagcgggtg ccgtgttcgg
     7741 cggccatggc gcccagggcg ccccgtgcgg cgacgagctc ggagcgcacc tcggccatgg
     7801 agcggcagac gggggtggcg atctcgacct ggaagtcggt cagctcggcc tggaggtcga
     7861 agcggccgtc cgacttggtc gccagcacct ccggcgcgag cggggcgacc tcgcgggtgg
     7921 tggcgtcgac cagcaggaac tcctcctcga cacccacgga gagggactgg tcgaagatgg
     7981 tccgcggtcg gggcaactgg ctggtgtcgg ccttgctcac gggatacgtc caatgctgtg
     8041 ggtagtcctc acaatgctat gagtgaccaa gccacaggca catgcggcgt attggcgaac
     8101 tctacgcgcg gatggcgtca gtttcggaca tccccaggcc ggcggccggt tccgagcgct
     8161 cgttgacgcc tgtcccagcg cgctgtcggt cgtgatccgc tggtcacgcc gcaggtcggg
     8221 gggacccgct ctcaccaacc ggtgtgccgc gcagcaggtc gacccggtcg ttgcccggcc
     8281 gaccgagcac ccagctggtg ccccgcaacg acttcgcccg cgccttcaac ggcgccagca
     8341 gccgcgacac ctcgcggtag gacggcaccg acccggcggc gcacaccagc gtcgccagcg
     8401 acagcgtgac ggccaggccc tcggcttcga actcggtgtc cagcacccgc gaggccagcg
     8461 gcacgatctc gtccaggccc gccacgacca ggaagtcgtc cccgccgacg tgccccacct
     8521 gcgcggtcgg ccacgggacc gccgcctcgg acagcgtccg tccgatcccg cggatcagct
     8581 cgtcacccgc ggcgaagccg accccgtcgt tgacccgctt gaacccgtcc acgtccagcc
     8641 agccgacggc gaagatctca cccgccatga tccgccggtc gacctcccgc gcgatcgcgt
     8701 cgctgccggg cagcctggtc agcgggttca gcgcggcggc ctgctccacc ttcagctcgg
     8761 cgatgccccg cacgacgtcc gcgacccgca cgaccccgag gcagcggtcg tcggcgtcga
     8821 cgacgaccag gtcgtcgttg gtgcgctcgc ggtcggtgtt ggcgacgatg tccagcagct
     8881 ccacggcgct ggtgccggtg ccgatcaggt gcggccggtc ggccagcctg gccgcgtcgc
     8941 ggcgggcgtg cagcgcgtgg ccgtaggggc cggtcacggc gaggaggaac cggttgcggt
     9001 ccaccgacca gcgcggacgg ccgtcggggt cgaccagcac gacaccgctg aggctcgggt
     9061 ggccagccag cagctcgcgc acccgctcgg acgtcgcgtc ctccggcaac gtggtcgcgg
     9121 ggtgcaggaa gtcggtcacc cgaggtccgg tggcgcgccg gatcgggccg gtgcgggtca
     9181 ccgcctccgg gtcgttggcc tcgctgagca cggccgcgat ggtggcgtcg accctcggtc
     9241 ttcgcgacgc cgccgccagc aggttgccct gcgccaacca caccccgagc ctgcgcaacg
     9301 acgccagctc ggcctcggtc tccacgccct cggcgaccag ctgcgccccg gtgtgctcgg
     9361 cgatgtgcag caacccctgg accaccgcgc actgaccggg gtcgtccggc agcgccgcca
     9421 cgacctcgcg gtccagcttc agcaggtccg gccgcgcccc ggcgagcagg ctcaacggca
     9481 cgtcgccctc acccaccccg tccagtgcga tccggaaccc gttcccgcgc aacagctcca
     9541 gcccgcgcag cagcgcgccc cgccgcgcgc gggagtaggg gatgccgacc tcgacgacca
     9601 cgctcgacgg cgaccggccc gcgtcgcgca gggcaccgaa cagcggggcg aggaactccg
     9661 gccggtcggc cagcgtcatc gccatcaggt tgaggtgcag cggcagcgcg agctcgtggt
     9721 cggcggcgga cccgaccgca cggcaggcga gcgccacgtc ggtgttcgtc aggtaccccg
     9781 cgccgtgggc cgcgcgcagc aggtcctgca cgccgccgtc atgaggccgg gccagggctt
     9841 cgaccgcgac gacacccccg gtgtggaggt tgaacagcgg ctggaaggcg aatcggacgt
     9901 catccagcaa cgcgggcacc cgtcgatcgt gcctggtcga tcatggtttg ccgaggtcgt
     9961 tcaccaggtg ttcacgacct cttcgggtgc ggtcgccccc tcagcggtcc tggggagcca
    10021 gcaccatcgt ccggcgcaac cgccacgggg caccgcggaa cagctcggcc atcgccttcg
    10081 tcgcccccgc gacgtcctcc cggtccgaca ggtaggcgcg ccgcagctcc tcggggagcc
    10141 cgaagaacag gtcgaagaac tccggcacgt gctcgggcgg catcgccagc agcacgcgca
    10201 gcccgtggcg gcgcatcgtg tgcacgacct tcgctgcggg cgaccagacg acctgccgcg
    10261 ccgcggcccg cgcggcacgg ggaccgcggg gcagcgcgtc ggcgatcgcg gcggcgacca
    10321 ggggagcgag cgtcaacgtc gtggcgacgc tgtacccggt ggccgggtgc accagcgcgg
    10381 ccgccgcgcc gaacgcgagc ttcgccgggc ggttgaggtc caccgggaag cggacctgct
    10441 cctccacgtc ggacaggtcg acgccgtgct gggccagccg cgcggccagc cgggtgcgca
    10501 gctcggctac cgggaggccg gggcgacggg ccagcgacgt ctcctccagc agcacgcggt
    10561 cgccgtccag ggggacggcg tagaggaagg tgggccagtc gacgccggtg gtcggtggct
    10621 gccgccagtc catgatcagc gcctcgccct cggcgaccaa ggccttcgcg tccgtggcgg
    10681 cgacgaccac cccggcggcg gtctgctcca ccctgccccg gctcggcggg gcaccggtgg
    10741 catcgaccac cagcgccgcg gtcagcgttc ggccgtcggc cagcagcacg gtcgtccccc
    10801 tgggaccgac aacggtgcgc acgcccttgc cggtctccac caggacgtcg gggtgcggca
    10861 cctggagccg cgcgttgtcg atgacggcgt acgcgcgatc cagcacgtgg gcctcgacgc
    10921 caatggcgcg ggtaaccggg gcgacagcgg ccaccggggt gcccgacggc aattcatcgg
    10981 cccacgcccc gtaggtcgcg gtccacggcc gttccggacg cgggtcgacc agcgtcgtct
    11041 ccagtcccga agtcgcacag gcacccgcca gcgcccggcc cgcgggtcca ccacccagca
    11101 cgatcacgtc cacactccga cgttatccct ccccgtgcgc gctccgccgc gcccgatcgg
    11161 gggtgatcgc gcacgtcaac gggccgtcga cccgaaaggg gacccccgat ttggagcccg
    11221 aaccacaccg gtgtaagttt ctccctgcca gcgcggaacg ggccggaaac ggaacggagc
    11281 ggggcaaaac cccagaacta catgaacacc agcacccctt gctgggccgg tgaagtcggt
    11341 ggttatctcc actgcaagcc gatttgacaa gggaaatgcg gttcggatag cgtctggaac
    11401 atagaaaact gaatgcgaag cgagtttctg ctgagcgaaa cggcccgaac cg
//
